MC01g1502 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1502 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 9.3e-253
Identity = 415/559 (74.24%), Postives = 482/559 (86.23%), Query Frame = 0

Query: 17  STSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQLDDAHTRDLVLTDGVM 76
           S  S +ALT TL N+IQALGRGFDVT+D+RLLYCKG PGSRL+++++   RDL L+ G +
Sbjct: 10  SVPSSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFL 69

Query: 77  VPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVD 136
           +PNVP DI CS  N  T+ + VC+FH+MAE FN +SG+ GNIPLG F+AMFN++GSWQVD
Sbjct: 70  LPNVPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVD 129

Query: 137 AAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSA 196
           AA+TKSLA+VGY IPL+ V+L    LVL  +I+RAVP +WDP SLASFIEN+GTHI+TS 
Sbjct: 130 AASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSV 189

Query: 197 TIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRR 256
           TIGGRDVVYIRQHQSSPL  SEIE+YV ++ + RF +++SQS  GPL YKDKD+TVIFRR
Sbjct: 190 TIGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRR 249

Query: 257 RGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIED 316
           RGGDDL QSHA+WA+TV  APD+INMTFTPIVS+LEGVPG++HL RAI+LYLEYKPPIED
Sbjct: 250 RGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIED 309

Query: 317 LQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNL 376
           LQYFLD+QI RAWAPEQ+NLQRKEP+C SLQFSLMGPKL+IS DQ+TVGRKPVTGLRL+L
Sbjct: 310 LQYFLDYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSL 369

Query: 377 EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVS 436
           EG KQNRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVS
Sbjct: 370 EGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVS 429

Query: 437 TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPS 496
           T+PIE+TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P 
Sbjct: 430 TSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPV 489

Query: 497 IPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAK 556
             +  L   G S+SS T       E+ S  +GKLAKIVD +EM KGPQD+PGHWLVTGAK
Sbjct: 490 ASSGRLEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAK 549

Query: 557 LGVDKGRIVLRIKYSLLNY 576
           LGV+KG+IVLR+KYSLLNY
Sbjct: 550 LGVEKGKIVLRVKYSLLNY 561

BLAST of MC01g1502 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.8e-123
Identity = 248/590 (42.03%), Postives = 352/590 (59.66%), Query Frame = 0

Query: 31  SIQALGRGFDVTADIRLLYCK-GTPGSRLIQLDDAHTR-DLVLTDGVMVPNVPDDIQCSS 90
           +I ++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G+ +PNV   I+C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 91  DNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAATKSLAMVGY 150
             R      +  F +MAE FNQ+  ++G IP G F+AMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 151 LIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 210
            I L+ V L  S ++LRE +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 211 HQSSPLSESEIEHYVKEVGEQRFLDSK--------------------------SQSTVGP 270
             SS L   +++  +KEV ++RF+++                             S++G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 271 LSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLAR 330
            + K+  V +  RR G D+    H +W  TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 331 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL----QRKEPLCPSLQFSLMGPKLYIS 390
           AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C SLQFS  GPKLY++
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 391 PDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEE 450
              + VG++P+TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQES 433

Query: 451 QDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLF 510
            D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L F
Sbjct: 434 HDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLCF 493

Query: 511 SKVPGCT-IRRSVWDHS------PSIPATPLRSDGASSSSKTRTSEDKKEDSS------- 570
           S+V G T ++ S WD +        + +T +     ++        D   +S+       
Sbjct: 494 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 553

Query: 571 --SNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSL 573
             + A KL K VD +EM++GPQ+ PG+W+V+GA+L V+KG+I L++KYSL
Sbjct: 554 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of MC01g1502 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.6e-114
Identity = 239/609 (39.24%), Postives = 342/609 (56.16%), Query Frame = 0

Query: 21  MDALTATLSNSIQALGRGFDVTADIRLLYCKGTP-GSRLIQLDDAHTRDLVLTDGVMVPN 80
           +DA +A    ++  +G G+D+ +D+R   CK TP GSRL+++D    RDL+   G++V N
Sbjct: 9   LDAHSAA-EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNN 68

Query: 81  VPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAA 140
           V   I+C    R      + +F++M+E FNQ   +SG IP G F+ MF FS  W  DA++
Sbjct: 69  VSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASS 128

Query: 141 TKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 200
            K+LA  G+ I L+ VE+    L LR+++KR VP +WD  +LA FIE +GTH++   T+G
Sbjct: 129 VKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMG 188

Query: 201 GRDVVYIRQHQSSPLSESEIEHYVKEVGEQRF----LDSKSQSTVGP------------- 260
           G+DV++++Q + S     EI+  +K  G++RF    ++SKS ++V               
Sbjct: 189 GKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGL 248

Query: 261 ------------LSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSM 320
                       +  K++++  +  RRGG DL QSH +W  TV  AP+VI+M F PI S+
Sbjct: 249 QPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSL 308

Query: 321 LEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPE----QTNLQRKEPLCPSL 380
           L G+PG   L+ A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +   PSL
Sbjct: 309 LSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSL 368

Query: 381 QFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSH 440
           QFSLMGPKLY++  ++  G +PVTGLR  LEG K N LAIHLQHL + P  L   H D++
Sbjct: 369 QFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTY 428

Query: 441 VAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWD 500
             I  P   G       ++ P+KW  FSHV T P++Y  A   D +   IVT A L V  
Sbjct: 429 EPIEEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKG 488

Query: 501 FGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTR----------- 560
            G + VL+L+L FS       R+S WD+     +T  R  G  S   TR           
Sbjct: 489 MGMRKVLFLRLGFSLDASAVTRKSCWDNL----STNSRKSGVFSMISTRLSTGLSPNPAT 548

Query: 561 TSEDKKEDSSSNA----------GKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRI 574
           T    K D +S             KL  +VD  E+ +GP++ PG+W+VTGAKL V+ G+I
Sbjct: 549 TKPQSKIDINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKI 603

BLAST of MC01g1502 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 4.6e-111
Identity = 243/619 (39.26%), Postives = 341/619 (55.09%), Query Frame = 0

Query: 28  LSNSIQALGRGFDVTADIRLLYCKGTPGS----RLIQLDDAHTRDLVLTDGVMVPNVPDD 87
           +  ++++LG+GFD+TAD RL YCK   GS    RL+ LD    R+L +    +  NV  D
Sbjct: 9   IETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSAD 68

Query: 88  IQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNF-SGSWQVDAAATKS 147
           I C    R      +  F+KM+EYFNQ+S ++G IP G+F+A F F SGSW  DAA  KS
Sbjct: 69  INCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKS 128

Query: 148 LAMVGYLIPLFKVELKNSN-LVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGR 207
           L +   ++ LF + + N N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++GG+
Sbjct: 129 LGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQ 188

Query: 208 DVVYIRQHQSSPLSESEIEHYVKEVGEQRF------------------------------ 267
           DVV +RQ +SS L    + H++ ++G+Q F                              
Sbjct: 189 DVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNV 248

Query: 268 LDSKSQSTVGPLSYKDKD-VTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSM 327
            D K        S   ++ +TVI  +RGGD   +SH++W  TV   PD IN  F PI S+
Sbjct: 249 FDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSL 308

Query: 328 LEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL-----QRKEPLCPS 387
           L+ VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L            P+
Sbjct: 309 LKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPA 368

Query: 388 LQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHWDS 447
           L  + MGPKLY++   +T  + PVTG+R  LEG K NRLAIHLQHL +    + +   D 
Sbjct: 369 LHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDE 428

Query: 448 HVAIGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV-HI 507
           H+      W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V  I
Sbjct: 429 HI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFI 488

Query: 508 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRT 567
           VTGAQL V   G+K+VL+L+L ++KV    + ++ W H P    T  +S   SS S   T
Sbjct: 489 VTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPI--GTSQKSGIFSSMSMPLT 548

Query: 568 S---------EDKKE---DSSSNAG--------KLAKIVDLTEMSKGPQDIPGHWLVTGA 575
           S         +DK E   DS    G        K+ K VDL+++ +GPQ  PGHWLVTG 
Sbjct: 549 SGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGV 608

BLAST of MC01g1502 vs. NCBI nr
Match: XP_022142258.1 (MACPF domain-containing protein CAD1 [Momordica charantia])

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ
Sbjct: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
           GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575

BLAST of MC01g1502 vs. NCBI nr
Match: XP_008439705.1 (PREDICTED: MACPF domain-containing protein CAD1 [Cucumis melo] >TYK13232.1 MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 1088 bits (2814), Expect = 0.0
Identity = 540/575 (93.91%), Postives = 556/575 (96.70%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP A+  TAA TATS  +MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPRATYQTAAATATS--NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQ+DAAATKSLAM+GY IP FKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS  
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAA 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSS+TRTSEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of MC01g1502 vs. NCBI nr
Match: XP_038883129.1 (MACPF domain-containing protein CAD1 [Benincasa hispida])

HSP 1 Score: 1083 bits (2800), Expect = 0.0
Identity = 539/575 (93.74%), Postives = 553/575 (96.17%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP  +QT AA    +TS+MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+ 
Sbjct: 1   MKDPRTTQTAAA---IATSNMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVH 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDA TRDLVL+DGV+VPNVPDD+QCSSD+RATENVPVC+FHKMAEYFNQKS ISGNIPL
Sbjct: 61  LDDARTRDLVLSDGVVVPNVPDDVQCSSDSRATENVPVCSFHKMAEYFNQKSDISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAM+GY IPLFKV LKNSNLVL EDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKNSNLVLCEDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQST 
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSELEIEHYVKEVGEQRFLDSKSQSTA 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 SPLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSSKTR SEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSKTRISEDKKEDSSTNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 572

BLAST of MC01g1502 vs. NCBI nr
Match: XP_011658263.1 (MACPF domain-containing protein CAD1 [Cucumis sativus] >KAE8647726.1 hypothetical protein Csa_003370 [Cucumis sativus])

HSP 1 Score: 1077 bits (2784), Expect = 0.0
Identity = 535/575 (93.04%), Postives = 552/575 (96.00%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP A+  T+A  AT TS+MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPRATYQTSA--ATPTSNMDALTTTLRNSIAALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAH RDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FHKMAEYFNQKS ISGNIPL
Sbjct: 61  LDDAHNRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSDISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAM+GY IPLFKV LK+SNLVL EDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQVDAAATKSLAMIGYYIPLFKVALKSSNLVLCEDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYV+EVGEQRF DSKS+ST 
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVEEVGEQRFFDSKSRSTA 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
            RAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPD
Sbjct: 301 VRAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSS+TRTSEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573

BLAST of MC01g1502 vs. NCBI nr
Match: KAA0052593.1 (MACPF domain-containing protein CAD1 [Cucumis melo var. makuwa])

HSP 1 Score: 1073 bits (2776), Expect = 0.0
Identity = 540/602 (89.70%), Postives = 556/602 (92.36%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP A+  TAA TATS  +MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPRATYQTAAATATS--NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQ+DAAATKSLAM+GY IP FKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS  
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAA 240

Query: 241 GPLSYKDK---------------------------DVTVIFRRRGGDDLVQSHAKWADTV 300
            PLSYKDK                           DVTVIFRRRGGDDLVQSHA+WA TV
Sbjct: 241 APLSYKDKVSRQQASSLFSWTVFSEHFTHDPSFLQDVTVIFRRRGGDDLVQSHAQWAKTV 300

Query: 301 KTAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQ 360
           K+APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQ
Sbjct: 301 KSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQ 360

Query: 361 TNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVS 420
           TNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVS
Sbjct: 361 TNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVS 420

Query: 421 LPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGV 480
           LPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGV
Sbjct: 421 LPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGV 480

Query: 481 HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSIPATPLRSDGASSSSKT 540
           HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPS PATPLRSDGASSSS+T
Sbjct: 481 HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSRT 540

Query: 541 RTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLL 575
           RTSEDKKEDSS+NAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLL
Sbjct: 541 RTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLL 600

BLAST of MC01g1502 vs. ExPASy TrEMBL
Match: A0A6J1CMV2 (MACPF domain-containing protein CAD1 OS=Momordica charantia OX=3673 GN=LOC111012420 PE=4 SV=1)

HSP 1 Score: 1154 bits (2985), Expect = 0.0
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ
Sbjct: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
           GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575

BLAST of MC01g1502 vs. ExPASy TrEMBL
Match: A0A5D3CPE6 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008470 PE=4 SV=1)

HSP 1 Score: 1088 bits (2814), Expect = 0.0
Identity = 540/575 (93.91%), Postives = 556/575 (96.70%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP A+  TAA TATS  +MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPRATYQTAAATATS--NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQ+DAAATKSLAM+GY IP FKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS  
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAA 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSS+TRTSEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of MC01g1502 vs. ExPASy TrEMBL
Match: A0A1S3AZC7 (MACPF domain-containing protein CAD1 OS=Cucumis melo OX=3656 GN=LOC103484421 PE=4 SV=1)

HSP 1 Score: 1088 bits (2814), Expect = 0.0
Identity = 540/575 (93.91%), Postives = 556/575 (96.70%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP A+  TAA TATS  +MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPRATYQTAAATATS--NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQ+DAAATKSLAM+GY IP FKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS  
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAA 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
            PLSYKDKDVTVIFRRRGGDDLVQSHA+WA TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 APLSYKDKDVTVIFRRRGGDDLVQSHAQWAKTVKSAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCTIRRSVWDHSPS PATPLRSDGASSSS+TRTSEDKKEDSS+NAGKLAKIVDLTEMS
Sbjct: 481 VPGCTIRRSVWDHSPSTPATPLRSDGASSSSRTRTSEDKKEDSSTNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLLNY 573

BLAST of MC01g1502 vs. ExPASy TrEMBL
Match: A0A5A7U9L9 (MACPF domain-containing protein CAD1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G002000 PE=4 SV=1)

HSP 1 Score: 1073 bits (2776), Expect = 0.0
Identity = 540/602 (89.70%), Postives = 556/602 (92.36%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP A+  TAA TATS  +MDALT TL NSI ALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPRATYQTAAATATS--NMDALTTTLCNSIGALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDAHTRDLVL+DGV+VPNVPDD+QCSSD RATENVPVC+FHKMAEYFNQKSGISGNIPL
Sbjct: 61  LDDAHTRDLVLSDGVVVPNVPDDVQCSSDTRATENVPVCSFHKMAEYFNQKSGISGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQ+DAAATKSLAM+GY IP FKV LK+SNLVLREDIKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQLDAAATKSLAMIGYYIPHFKVALKSSNLVLREDIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSE EIEHYVKEVGEQRFLDSKSQS  
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSEFEIEHYVKEVGEQRFLDSKSQSAA 240

Query: 241 GPLSYKDK---------------------------DVTVIFRRRGGDDLVQSHAKWADTV 300
            PLSYKDK                           DVTVIFRRRGGDDLVQSHA+WA TV
Sbjct: 241 APLSYKDKVSRQQASSLFSWTVFSEHFTHDPSFLQDVTVIFRRRGGDDLVQSHAQWAKTV 300

Query: 301 KTAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQ 360
           K+APDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQ
Sbjct: 301 KSAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQ 360

Query: 361 TNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVS 420
           TNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVS
Sbjct: 361 TNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVS 420

Query: 421 LPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGV 480
           LPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGV
Sbjct: 421 LPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGV 480

Query: 481 HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSIPATPLRSDGASSSSKT 540
           HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPS PATPLRSDGASSSS+T
Sbjct: 481 HIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSTPATPLRSDGASSSSRT 540

Query: 541 RTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLL 575
           RTSEDKKEDSS+NAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKG+IVLRIKYSLL
Sbjct: 541 RTSEDKKEDSSTNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGKIVLRIKYSLL 600

BLAST of MC01g1502 vs. ExPASy TrEMBL
Match: A0A6J1EGA5 (MACPF domain-containing protein CAD1 OS=Cucurbita moschata OX=3662 GN=LOC111433834 PE=4 SV=1)

HSP 1 Score: 1073 bits (2775), Expect = 0.0
Identity = 535/575 (93.04%), Postives = 549/575 (95.48%), Query Frame = 0

Query: 1   MKDPFASQTTAAGTATSTSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQ 60
           MKDP    T AA   T TS MDALTATL NSIQALGRGFDVTADIRLLYCKGTPGSRL+Q
Sbjct: 1   MKDPCT--TYAAAATTPTSEMDALTATLCNSIQALGRGFDVTADIRLLYCKGTPGSRLVQ 60

Query: 61  LDDAHTRDLVLTDGVMVPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPL 120
           LDDA TRDL L+DGV+VPNVPD + CSSD RATENVPVC+FHKMAEYFNQKS +SGNIPL
Sbjct: 61  LDDASTRDLHLSDGVVVPNVPDHVHCSSDTRATENVPVCSFHKMAEYFNQKSNLSGNIPL 120

Query: 121 GSFDAMFNFSGSWQVDAAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVS 180
           GSF+AMFNFSGSWQVDAAATKSLAM+GY IPLFKVELKNS LVL E+IKRAVPYTWDPVS
Sbjct: 121 GSFNAMFNFSGSWQVDAAATKSLAMIGYFIPLFKVELKNSTLVLHEEIKRAVPYTWDPVS 180

Query: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTV 240
           LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIE+YVKEVGEQRFLDSKSQSTV
Sbjct: 181 LASFIENFGTHIITSATIGGRDVVYIRQHQSSPLSESEIENYVKEVGEQRFLDSKSQSTV 240

Query: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHL 300
           GPLSYKDKDVTVIFRRRGGDDLVQSHAKWA+TVK+APDVINMTFTPIVSMLEGVPGVKHL
Sbjct: 241 GPLSYKDKDVTVIFRRRGGDDLVQSHAKWAETVKSAPDVINMTFTPIVSMLEGVPGVKHL 300

Query: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPD 360
           ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLC SLQFSLMGPKLYISPD
Sbjct: 301 ARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNLQRKEPLCSSLQFSLMGPKLYISPD 360

Query: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQD 420
           QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGA KWHGPEEQD
Sbjct: 361 QITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGALKWHGPEEQD 420

Query: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480
           SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK
Sbjct: 421 SRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSK 480

Query: 481 VPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMS 540
           VPGCT+RRSVWDHSPS  ATP RSDGASSSSKT+T EDKKEDSSSNAGKLAKIVDLTEMS
Sbjct: 481 VPGCTVRRSVWDHSPSTSATPSRSDGASSSSKTKTGEDKKEDSSSNAGKLAKIVDLTEMS 540

Query: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 575
           KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY
Sbjct: 541 KGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSLLNY 573

BLAST of MC01g1502 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 874.0 bits (2257), Expect = 6.6e-254
Identity = 415/559 (74.24%), Postives = 482/559 (86.23%), Query Frame = 0

Query: 17  STSSMDALTATLSNSIQALGRGFDVTADIRLLYCKGTPGSRLIQLDDAHTRDLVLTDGVM 76
           S  S +ALT TL N+IQALGRGFDVT+D+RLLYCKG PGSRL+++++   RDL L+ G +
Sbjct: 10  SVPSSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFL 69

Query: 77  VPNVPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVD 136
           +PNVP DI CS  N  T+ + VC+FH+MAE FN +SG+ GNIPLG F+AMFN++GSWQVD
Sbjct: 70  LPNVPADIDCSRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVD 129

Query: 137 AAATKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSA 196
           AA+TKSLA+VGY IPL+ V+L    LVL  +I+RAVP +WDP SLASFIEN+GTHI+TS 
Sbjct: 130 AASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSV 189

Query: 197 TIGGRDVVYIRQHQSSPLSESEIEHYVKEVGEQRFLDSKSQSTVGPLSYKDKDVTVIFRR 256
           TIGGRDVVYIRQHQSSPL  SEIE+YV ++ + RF +++SQS  GPL YKDKD+TVIFRR
Sbjct: 190 TIGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDKDITVIFRR 249

Query: 257 RGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLARAIDLYLEYKPPIED 316
           RGGDDL QSHA+WA+TV  APD+INMTFTPIVS+LEGVPG++HL RAI+LYLEYKPPIED
Sbjct: 250 RGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIED 309

Query: 317 LQYFLDFQIGRAWAPEQTNLQRKEPLCPSLQFSLMGPKLYISPDQITVGRKPVTGLRLNL 376
           LQYFLD+QI RAWAPEQ+NLQRKEP+C SLQFSLMGPKL+IS DQ+TVGRKPVTGLRL+L
Sbjct: 310 LQYFLDYQIARAWAPEQSNLQRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSL 369

Query: 377 EGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEEQDSRWFEPIKWKNFSHVS 436
           EG KQNRL+IHLQHLVSLPKILQPHWDSHV IGAPKW GPEEQDSRWFEPIKWKNFSHVS
Sbjct: 370 EGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVS 429

Query: 437 TAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPS 496
           T+PIE+TE +IGDLSGVHIVTGAQLGVWDFG+KNVL+LKLLFSKVPGCTIRRSVWDH+P 
Sbjct: 430 TSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPV 489

Query: 497 IPATPLRSDGASSSSKTRTSEDKKEDSSSNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAK 556
             +  L   G S+SS T       E+ S  +GKLAKIVD +EM KGPQD+PGHWLVTGAK
Sbjct: 490 ASSGRLEPGGPSTSSST-------EEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAK 549

Query: 557 LGVDKGRIVLRIKYSLLNY 576
           LGV+KG+IVLR+KYSLLNY
Sbjct: 550 LGVEKGKIVLRVKYSLLNY 561

BLAST of MC01g1502 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-124
Identity = 248/590 (42.03%), Postives = 352/590 (59.66%), Query Frame = 0

Query: 31  SIQALGRGFDVTADIRLLYCK-GTPGSRLIQLDDAHTR-DLVLTDGVMVPNVPDDIQCSS 90
           +I ++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G+ +PNV   I+C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 91  DNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAATKSLAMVGY 150
             R      +  F +MAE FNQ+  ++G IP G F+AMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 151 LIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 210
            I L+ V L  S ++LRE +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 211 HQSSPLSESEIEHYVKEVGEQRFLDSK--------------------------SQSTVGP 270
             SS L   +++  +KEV ++RF+++                             S++G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 271 LSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLAR 330
            + K+  V +  RR G D+    H +W  TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313

Query: 331 AIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL----QRKEPLCPSLQFSLMGPKLYIS 390
           AI+LYL YKPPIE+L  FL+FQ+ R WAP  + L    QRK+  C SLQFS  GPKLY++
Sbjct: 314 AINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVN 373

Query: 391 PDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQPHWDSHVAIGAPKWHGPEE 450
              + VG++P+TG+RL LEG + NRLAIHLQHL SLPKI Q   D + +I        E 
Sbjct: 374 TTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI------RQES 433

Query: 451 QDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWDFGAKNVLYLKLLF 510
            D R++E + WKN+SHV T P+E  +    DLS   +VTGAQL V   G KNVL+L+L F
Sbjct: 434 HDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFKNVLFLRLCF 493

Query: 511 SKVPGCT-IRRSVWDHS------PSIPATPLRSDGASSSSKTRTSEDKKEDSS------- 570
           S+V G T ++ S WD +        + +T +     ++        D   +S+       
Sbjct: 494 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 553

Query: 571 --SNAGKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRIVLRIKYSL 573
             + A KL K VD +EM++GPQ+ PG+W+V+GA+L V+KG+I L++KYSL
Sbjct: 554 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of MC01g1502 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.9e-115
Identity = 239/609 (39.24%), Postives = 342/609 (56.16%), Query Frame = 0

Query: 21  MDALTATLSNSIQALGRGFDVTADIRLLYCKGTP-GSRLIQLDDAHTRDLVLTDGVMVPN 80
           +DA +A    ++  +G G+D+ +D+R   CK TP GSRL+++D    RDL+   G++V N
Sbjct: 9   LDAHSAA-EKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNN 68

Query: 81  VPDDIQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAA 140
           V   I+C    R      + +F++M+E FNQ   +SG IP G F+ MF FS  W  DA++
Sbjct: 69  VSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASS 128

Query: 141 TKSLAMVGYLIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIG 200
            K+LA  G+ I L+ VE+    L LR+++KR VP +WD  +LA FIE +GTH++   T+G
Sbjct: 129 VKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMG 188

Query: 201 GRDVVYIRQHQSSPLSESEIEHYVKEVGEQRF----LDSKSQSTVGP------------- 260
           G+DV++++Q + S     EI+  +K  G++RF    ++SKS ++V               
Sbjct: 189 GKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGL 248

Query: 261 ------------LSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSM 320
                       +  K++++  +  RRGG DL QSH +W  TV  AP+VI+M F PI S+
Sbjct: 249 QPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSL 308

Query: 321 LEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPE----QTNLQRKEPLCPSL 380
           L G+PG   L+ A++LYL YKPPIE+L  FL+FQ+ R WAP        L+R +   PSL
Sbjct: 309 LSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSL 368

Query: 381 QFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSLPKILQ-PHWDSH 440
           QFSLMGPKLY++  ++  G +PVTGLR  LEG K N LAIHLQHL + P  L   H D++
Sbjct: 369 QFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTY 428

Query: 441 VAIGAPKWHGPEEQDSRWFEPIKWKNFSHVSTAPIEYTEANIGDLSGVHIVTGAQLGVWD 500
             I  P   G       ++ P+KW  FSHV T P++Y  A   D +   IVT A L V  
Sbjct: 429 EPIEEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKG 488

Query: 501 FGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTR----------- 560
            G + VL+L+L FS       R+S WD+     +T  R  G  S   TR           
Sbjct: 489 MGMRKVLFLRLGFSLDASAVTRKSCWDNL----STNSRKSGVFSMISTRLSTGLSPNPAT 548

Query: 561 TSEDKKEDSSSNA----------GKLAKIVDLTEMSKGPQDIPGHWLVTGAKLGVDKGRI 574
           T    K D +S             KL  +VD  E+ +GP++ PG+W+VTGAKL V+ G+I
Sbjct: 549 TKPQSKIDINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKI 603

BLAST of MC01g1502 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 403.3 bits (1035), Expect = 3.3e-112
Identity = 243/619 (39.26%), Postives = 341/619 (55.09%), Query Frame = 0

Query: 28  LSNSIQALGRGFDVTADIRLLYCKGTPGS----RLIQLDDAHTRDLVLTDGVMVPNVPDD 87
           +  ++++LG+GFD+TAD RL YCK   GS    RL+ LD    R+L +    +  NV  D
Sbjct: 9   IETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSAD 68

Query: 88  IQCSSDNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNF-SGSWQVDAAATKS 147
           I C    R      +  F+KM+EYFNQ+S ++G IP G+F+A F F SGSW  DAA  KS
Sbjct: 69  INCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKS 128

Query: 148 LAMVGYLIPLFKVELKNSN-LVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGR 207
           L +   ++ LF + + N N L L + ++ AVP +WDP  LA FIE +GTH+IT  ++GG+
Sbjct: 129 LGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQ 188

Query: 208 DVVYIRQHQSSPLSESEIEHYVKEVGEQRF------------------------------ 267
           DVV +RQ +SS L    + H++ ++G+Q F                              
Sbjct: 189 DVVVVRQDKSSDLDNDLLRHHLYDLGDQLFTGSCLLSTRRLNKAYHHSHSQPKFPEAFNV 248

Query: 268 LDSKSQSTVGPLSYKDKD-VTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSM 327
            D K        S   ++ +TVI  +RGGD   +SH++W  TV   PD IN  F PI S+
Sbjct: 249 FDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPITSL 308

Query: 328 LEGVPGVKHLARAIDLYLEYKPPIEDLQYFLDFQIGRAWAPEQTNL-----QRKEPLCPS 387
           L+ VPG   L+ A+ LYL YKPP+ DLQYFLDF   RAWAP   +L            P+
Sbjct: 309 LKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPA 368

Query: 388 LQFSLMGPKLYISPDQITVGRKPVTGLRLNLEGCKQNRLAIHLQHLVSL-PKILQPHWDS 447
           L  + MGPKLY++   +T  + PVTG+R  LEG K NRLAIHLQHL +    + +   D 
Sbjct: 369 LHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDE 428

Query: 448 HVAIGAPKWHGPEE--QDSRWFEPIKWKNFSHVSTAPIEY------TEANIGDLSGV-HI 507
           H+      W G ++   + R+FEP+  K FSHV T P++Y      T +N    + V  I
Sbjct: 429 HI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFI 488

Query: 508 VTGAQLGVWDFGAKNVLYLKLLFSKVPGCTIRRSVWDHSPSIPATPLRSDGASSSSKTRT 567
           VTGAQL V   G+K+VL+L+L ++KV    + ++ W H P    T  +S   SS S   T
Sbjct: 489 VTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPI--GTSQKSGIFSSMSMPLT 548

Query: 568 S---------EDKKE---DSSSNAG--------KLAKIVDLTEMSKGPQDIPGHWLVTGA 575
           S         +DK E   DS    G        K+ K VDL+++ +GPQ  PGHWLVTG 
Sbjct: 549 SGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCRGPQHSPGHWLVTGV 608

BLAST of MC01g1502 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 234.6 bits (597), Expect = 2.0e-61
Identity = 124/310 (40.00%), Postives = 180/310 (58.06%), Query Frame = 0

Query: 31  SIQALGRGFDVTADIRLLYCK-GTPGSRLIQLDDAHTR-DLVLTDGVMVPNVPDDIQCSS 90
           +I ++G G+D+  D+RL YCK G+  SRL+ + +     ++VL  G+ +PNV   I+C  
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 91  DNRATENVPVCTFHKMAEYFNQKSGISGNIPLGSFDAMFNFSGSWQVDAAATKSLAMVGY 150
             R      +  F +MAE FNQ+  ++G IP G F+AMF FS  WQ DAA TK+LA  G 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 151 LIPLFKVELKNSNLVLREDIKRAVPYTWDPVSLASFIENFGTHIITSATIGGRDVVYIRQ 210
            I L+ V L  S ++LRE +K+AVP TWDP +LA FI+ +GTHII S  +GG+DV+Y +Q
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 211 HQSSPLSESEIEHYVKEVGEQRFLDSK--------------------------SQSTVGP 270
             SS L   +++  +KEV ++RF+++                             S++G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 271 LSYKDKDVTVIFRRRGGDDLVQSHAKWADTVKTAPDVINMTFTPIVSMLEGVPGVKHLAR 313
            + K+  V +  RR G D+    H +W  TV+  PDVI+M+F PI S+L GVPG   L+ 
Sbjct: 254 YANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSH 313