MC01g1485 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1485 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.8e-91
Identity = 177/384 (46.09%), Postives = 245/384 (63.80%), Query Frame = 0

Query: 7   SSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPHKSCFAP 66
           +S+ SL+IF  +       +       + SI +FGDS++DTGNL       +   + F P
Sbjct: 2   ASLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFPP 61

Query: 67  YGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNFAVAGAT 126
           YGETFF  PTGR  DGR+I+DFIAE +GLP V PY G + +     +F+KG+NFAVAGAT
Sbjct: 62  YGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNR-----NFDKGVNFAVAGAT 121

Query: 127 ALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVVVGEIGG 186
           AL +SFL++    + HTN SL VQL+ F+ +  ++C S S   CR+++ ++L+++GEIGG
Sbjct: 122 ALKSSFLKKR-GIQPHTNVSLGVQLKSFKKSLPNLCGSPSD--CRDMIGNALILMGEIGG 181

Query: 187 NDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNPTYLKLY 246
           NDYN PFF R+  ++++ LVP V+  I+STI ELI +G +T +VPG  PIGC+  YL LY
Sbjct: 182 NDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLY 241

Query: 247 GTSVKNS---ENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRFFNA 306
            TS K+      GCL+WLN+F EYH+E LK EL ++R  YPHV++IYADY+NS +R F  
Sbjct: 242 KTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKE 301

Query: 307 PQDFGF-TNTLQSCLVL----EDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEA 366
           P  FGF      +C  +        ++K  + G+        C DPSKYV WDG+H+TEA
Sbjct: 302 PAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGV------KSCKDPSKYVGWDGVHMTEA 361

Query: 367 AYRAIAIGLLQGPYTIPQITTSCI 383
           AY+ IA G+L GPY  P    SC+
Sbjct: 362 AYKWIADGILNGPYANPPFDRSCL 371

BLAST of MC01g1485 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.7e-91
Identity = 178/357 (49.86%), Postives = 236/357 (66.11%), Query Frame = 0

Query: 34  YSSIFNFGDSLSDTGNLFDECSSGEPHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESL 93
           + SI +FGDS++DTGNL          K  F PYGETFF  PTGR S+GRLI+DFIAE L
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 94  GLPLVQPYLGVRRKGITAEDFEKGLNFAVAGATALDASFLRENMSCKVHTNYSLAVQLEW 153
           G PLV P+ G +       +FEKG+NFAV GATAL+ SFL E      +TN SLAVQL  
Sbjct: 92  GFPLVPPFYGSQN-----ANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSS 151

Query: 154 FRDAYSSVCRSSSTARCREILKSSLVVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQI 213
           F+++  ++C S S   CR+++++SL+++GEIGGNDYN  FF  ++ E+IK LVPLV++ I
Sbjct: 152 FKESLPNLCVSPSD--CRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETI 211

Query: 214 ASTIIELIKLGAETLMVPGNLPIGCNPTYLKLYGTSVKNSE-----NGCLEWLNEFSEYH 273
           +S I ELI +G +T +VPG  P+GC+  YL LY TS  N E      GCL+WLN+FSEYH
Sbjct: 212 SSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTS--NIEEYDPLTGCLKWLNKFSEYH 271

Query: 274 NELLKEELKQIRANYPHVHLIYADYHNSAMRFFNAPQDFGF-TNTLQSCLVLEDQTS-KK 333
           +E L+ EL +++  YPHV++IYADY+N+ +R    P  FGF +  L +C  L    +   
Sbjct: 272 DEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPLPACCALGGPFNFTL 331

Query: 334 HTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAYRAIAIGLLQGPYTIPQITTSCIS 384
               G   P+    C DPSKYV WDG+H+TEAAYR +A G+L+GPY IP    SC+S
Sbjct: 332 GRKRGTQVPE---CCDDPSKYVSWDGVHMTEAAYRLMAEGILKGPYAIPPFDWSCLS 376

BLAST of MC01g1485 vs. ExPASy Swiss-Prot
Match: Q9ZQI3 (GDSL esterase/lipase At2g27360 OS=Arabidopsis thaliana OX=3702 GN=At2g27360 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 6.2e-91
Identity = 173/361 (47.92%), Postives = 234/361 (64.82%), Query Frame = 0

Query: 34  YSSIFNFGDSLSDTGNLFDECSSGEPHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESL 93
           + SI +FGDS++DTGNL    S  +  +S F PYGETFF  P+GR SDGRLI+DFIAE L
Sbjct: 31  FKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETFFHHPSGRFSDGRLIIDFIAEFL 90

Query: 94  GLPLVQPYLGVRRKGITAEDFEKGLNFAVAGATALDASFLRENMSCKVHTNYSLAVQLEW 153
           G+P V P+ G +       +FEKG+NFAV GATAL+ S L E  +    +N SL  QL+ 
Sbjct: 91  GIPHVPPFYGSKN-----GNFEKGVNFAVGGATALECSVLEEKGTHCSQSNISLGNQLKS 150

Query: 154 FRDAYSSVCRSSSTARCREILKSSLVVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQI 213
           F+++   +C SSS   CR++++++ +++GEIGGNDYN P F+R++ E++K LVPLV+  I
Sbjct: 151 FKESLPYLCGSSS-PDCRDMIENAFILIGEIGGNDYNFPLFDRKNIEEVKELVPLVITTI 210

Query: 214 ASTIIELIKLGAETLMVPGNLPIGCNPTYLKLYGTSVKNSEN---GCLEWLNEFSEYHNE 273
           +S I EL+ +GA T +VPGN P+GC+  YL LY T  K   N   GCL WLN+FS YHNE
Sbjct: 211 SSAISELVDMGARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVYHNE 270

Query: 274 LLKEELKQIRANYPHVHLIYADYHNSAMRFFNAPQDFGFTN-TLQSCLVLEDQTSKKHTN 333
            L+ ELK++R  YPHV++IY DY+N+ +R    P  FG  +  L +C  L       +  
Sbjct: 271 QLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQEPSKFGLMDRPLPACCGL---GGPYNFT 330

Query: 334 YGI-LPPKFKTVCADPSKYVCWDGIHLTEAAYRAIAIGLLQGPYTIPQITTSCISQNATT 390
           + I    K    C+DPSKYV WDGIH+TEAAY+ I+ G+L GPY IP    SC+      
Sbjct: 331 FSIKCGSKGVEYCSDPSKYVNWDGIHMTEAAYKWISEGVLTGPYAIPPFNWSCLDSKIKN 382

BLAST of MC01g1485 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 331.3 bits (848), Expect = 1.5e-89
Identity = 179/391 (45.78%), Postives = 252/391 (64.45%), Query Frame = 0

Query: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH 60
           +DS  +  ++  I+ ++ V+S  +   +     + SI +FGDS++DTGNL       +  
Sbjct: 4   LDSLPAMKLVRFILSTLLVTSVNS---QTQCRNFKSIISFGDSIADTGNLLGLSDPNDLP 63

Query: 61  KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF 120
            S F PYGETFF  PTGR SDGRLI+DFIAE LG PLV P+ G +       +F+KG+NF
Sbjct: 64  ASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQN-----ANFKKGVNF 123

Query: 121 AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVV 180
           AVAGATAL+ SFL E       TN SL+VQL  F ++  ++C S S   CR++++++L++
Sbjct: 124 AVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGSPSD--CRDMIENALIL 183

Query: 181 VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP 240
           +GEIGGNDYN   F+R+  ++++ LVP V+  I+S I EL+ +G  T +VPGN PIG + 
Sbjct: 184 MGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSA 243

Query: 241 TYLKLYGTSVKNSEN---GCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSA 300
           +YL LY TS K   +   GCL+WLN+FSEY+N+ L+EEL  +R  YPHV++IYADY+N+ 
Sbjct: 244 SYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNAL 303

Query: 301 MRFFNAPQDFGFTN-TLQSCLVL----EDQTSKKHTNYGILPPKFKTVCADPSKYVCWDG 360
           +R F  P  FGF N  L +C  +        S++  + G+        C DPS+YV +DG
Sbjct: 304 LRLFQEPAKFGFMNRPLPACCGVGGSYNFNFSRRCGSVGV------EYCDDPSQYVNYDG 363

Query: 361 IHLTEAAYRAIAIGLLQGPYTIPQITTSCIS 384
           IH+TEAAYR I+ GLL+GPY IP    SC+S
Sbjct: 364 IHMTEAAYRLISEGLLKGPYAIPPFKWSCLS 378

BLAST of MC01g1485 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.7e-88
Identity = 180/385 (46.75%), Postives = 241/385 (62.60%), Query Frame = 0

Query: 6   SSSIISLIIFSIAVSSAFAFAPRASGC-GYSSIFNFGDSLSDTGNLFDECSSGEPHKSCF 65
           +S I+S ++  I   +    A   S C  Y SI +FGDS++DTGN     +     ++ F
Sbjct: 8   ASLIVSFLL--ILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAF 67

Query: 66  APYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNFAVAG 125
            PYGE+FF  P+GR SDGRL++DFIAE LGLP V PY G +        F +G+NFAV G
Sbjct: 68  LPYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPYFGSQN-----VSFNQGINFAVYG 127

Query: 126 ATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVVVGEI 185
           ATALD +FL +       TN SL+VQL  F+    ++C +SST  CRE+L  SL+++GEI
Sbjct: 128 ATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPNLC-ASSTRDCREMLGDSLILMGEI 187

Query: 186 GGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNPTYLK 245
           GGNDYN PFFE +S  +IK LVPL++K I+S I++LI LG +T +VPGN PIGC+  YL 
Sbjct: 188 GGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLT 247

Query: 246 LYGTSVKNSE--NGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRFFN 305
           L+ T+    +   GC+ WLN+F E+HNE LK ELKQ++  YPHV++IYADY+NS    F 
Sbjct: 248 LFQTATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQ 307

Query: 306 APQDFGFTN-TLQSCLVLEDQ----TSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTE 365
            P  +GF N  L +C  +  Q      K+    G+      + C +PS+YV WDG HLTE
Sbjct: 308 EPAKYGFKNRPLAACCGVGGQYNFTIGKECGENGV------SYCQNPSEYVNWDGYHLTE 367

Query: 366 AAYRAIAIGLLQGPYTIPQITTSCI 383
           A Y+ +A GLL G YT P    SC+
Sbjct: 368 ATYQKMAQGLLNGRYTTPAFDWSCL 378

BLAST of MC01g1485 vs. NCBI nr
Match: XP_022142303.1 (GDSL esterase/lipase At1g28600 [Momordica charantia])

HSP 1 Score: 791 bits (2042), Expect = 1.16e-288
Identity = 393/394 (99.75%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH 60
           MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH
Sbjct: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH 60

Query: 61  KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF 120
           KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF
Sbjct: 61  KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF 120

Query: 121 AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVV 180
           AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLV+
Sbjct: 121 AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVL 180

Query: 181 VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP 240
           VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP
Sbjct: 181 VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP 240

Query: 241 TYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRF 300
           TYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRF
Sbjct: 241 TYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRF 300

Query: 301 FNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAY 360
           FNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAY
Sbjct: 301 FNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAY 360

Query: 361 RAIAIGLLQGPYTIPQITTSCISQNATTNLPQLL 394
           RAIAIGLLQGPYTIPQITTSCISQNATTNLPQLL
Sbjct: 361 RAIAIGLLQGPYTIPQITTSCISQNATTNLPQLL 394

BLAST of MC01g1485 vs. NCBI nr
Match: XP_038882470.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At1g28570-like [Benincasa hispida])

HSP 1 Score: 523 bits (1347), Expect = 5.91e-183
Identity = 274/401 (68.33%), Postives = 318/401 (79.30%), Query Frame = 0

Query: 1   MDSSNSSSIISL----IIFSIAVSSAFAFAPR--ASGCGYSSIFNFGDSLSDTGNLFDEC 60
           MDSSNSSS + L    IIFS+A+    A +P    + C ++SIFNFGDSLSDTGN+F +C
Sbjct: 1   MDSSNSSSFLWLNRWLIIFSVAI---IALSPPNILANC-FTSIFNFGDSLSDTGNVFYKC 60

Query: 61  SSGEPHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYL--GVRRKGITAE 120
            S +P +SCF PYG+TFFRRPTGR SDGRLI+DFIA+SLGLPL+QPYL  GV R+ ++ E
Sbjct: 61  QSNKPPRSCFLPYGDTFFRRPTGRYSDGRLIIDFIAQSLGLPLLQPYLDVGVERQRMSVE 120

Query: 121 DFEKGLNFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCRE 180
           +FEKGLNFAV GAT LDAS+LRE +  +V TNYSL+VQLEWFR+AYSSVC S+ST RCR+
Sbjct: 121 EFEKGLNFAVVGATTLDASYLREKLMVEVPTNYSLSVQLEWFRNAYSSVCASTSTTRCRK 180

Query: 181 ILKSSLVVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPG 240
           ILKSSL +VGEIGGNDYN PFF+R +FE+IKSLVPLVVK I STI ELI+LGA+ LMVPG
Sbjct: 181 ILKSSLFLVGEIGGNDYNYPFFDRHNFEEIKSLVPLVVKAIGSTITELIQLGAQILMVPG 240

Query: 241 NLPIGCNPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYAD 300
           NLPIGCNPTYLK Y TS+++S+NGCL+WLN+FSEYHNE L+EEL +I A YP V +IYAD
Sbjct: 241 NLPIGCNPTYLKTYSTSLQDSKNGCLDWLNQFSEYHNEQLQEELNRIXACYPDVQIIYAD 300

Query: 301 YHNSAMRFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDG 360
           Y+NSAMRFFN P+ FG TNTLQ CLV   +T            K KTVC DPSKYV WDG
Sbjct: 301 YYNSAMRFFNQPEKFGLTNTLQPCLVDTHET------------KTKTVCDDPSKYVSWDG 360

Query: 361 IHLTEAAYRAIAIGLLQGPYTIPQITTSCISQNATTNLPQL 393
           IHLTEAAY  IA  LLQGPYT PQ TTSCIS N T NL QL
Sbjct: 361 IHLTEAAYSIIANALLQGPYTYPQFTTSCISHNLTMNLLQL 385

BLAST of MC01g1485 vs. NCBI nr
Match: XP_022925815.1 (GDSL esterase/lipase At1g28590-like [Cucurbita moschata])

HSP 1 Score: 512 bits (1318), Expect = 1.94e-178
Identity = 268/397 (67.51%), Postives = 312/397 (78.59%), Query Frame = 0

Query: 1   MDSSNSSSIISLI-IFSIAVSSAFAFAPR-ASGCGYSSIFNFGDSLSDTGNLFDECSSGE 60
           M SSNSS + SL  +F + V S   FAP    GC ++SIFNFGDSLSDTGNLF  C S E
Sbjct: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGC-FTSIFNFGDSLSDTGNLFGNCVSEE 60

Query: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120
           P  SCF PYG+ FF  PTGR S+GRLI+DFIA+SLGLPL+ PYL V R+ I+ +DFEKGL
Sbjct: 61  PPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFEKGL 120

Query: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTA--RCREILKS 180
           NFAVAGATALD S LRE     + TNYSL VQ+EWF++ YSSVC+SSSTA  +CR IL+ 
Sbjct: 121 NFAVAGATALDESVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSSTATAKCRAILER 180

Query: 181 SLVVVGEIGGNDYNDPFF-ERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLP 240
           SL+VVGEIGGNDYN  F  ER++ ++IKSLV  VVK I S I ELIKLGA+TLMVPGNLP
Sbjct: 181 SLIVVGEIGGNDYNYAFIDERQNIDEIKSLVTPVVKAIGSAITELIKLGAQTLMVPGNLP 240

Query: 241 IGCNPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHN 300
           IGCNPTYLK+YGTSV+NSENGC+ WLNEFSEYHNE L +ELKQIRA YPH+ +IYADY+N
Sbjct: 241 IGCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQIRALYPHIRIIYADYYN 300

Query: 301 SAMRFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHL 360
           SAM F++APQDFG TNTLQ CLV E +TS  H  YG     + ++C +PS+YV WDG+HL
Sbjct: 301 SAMLFYDAPQDFGLTNTLQLCLVNEKETSNTHRKYG-----YSSLCDNPSEYVSWDGMHL 360

Query: 361 TEAAYRAIAIGLLQGPYTIPQITTSCISQNATTNLPQ 392
           TEAAY+ IAI LL+GPYT PQI TSCISQNATT L Q
Sbjct: 361 TEAAYKVIAIALLEGPYTTPQIKTSCISQNATTTLLQ 391

BLAST of MC01g1485 vs. NCBI nr
Match: XP_023544566.1 (GDSL esterase/lipase At1g28590-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511 bits (1317), Expect = 2.76e-178
Identity = 268/397 (67.51%), Postives = 310/397 (78.09%), Query Frame = 0

Query: 1   MDSSNSSSIISLI-IFSIAVSSAFAFAP-RASGCGYSSIFNFGDSLSDTGNLFDECSSGE 60
           M SSNSS ++SL  +F I V     FAP    GC ++SIFNFGDSLSDTGNLF  C S E
Sbjct: 1   MGSSNSSPVLSLNRLFIILVLPLLTFAPPTVFGC-FTSIFNFGDSLSDTGNLFGNCVSEE 60

Query: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120
           P  SCF PYG+ FF  PTGR S+GRLI+DFIA+SLGLPL+QPYLGV R+ I+ +DFEKGL
Sbjct: 61  PPHSCFPPYGDAFFHHPTGRFSNGRLIIDFIAQSLGLPLLQPYLGVGRRRISIQDFEKGL 120

Query: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTA--RCREILKS 180
           NFAVAGATALD + LRE     + TNYSL VQLEWF++ YS VC+SSSTA  +CR ILKS
Sbjct: 121 NFAVAGATALDEAVLREKGITNLPTNYSLGVQLEWFKNTYSFVCKSSSTATEKCRAILKS 180

Query: 181 SLVVVGEIGGNDYNDPFF-ERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLP 240
           SL+VVGEIGGNDYN PF  ER++ E+IKSLV  VVK I S I ELIKLGA+TLMVPGNLP
Sbjct: 181 SLIVVGEIGGNDYNYPFIDERQNIEEIKSLVAPVVKAIGSAITELIKLGAQTLMVPGNLP 240

Query: 241 IGCNPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHN 300
           IGCNPTYLK+YGTSV+NSENGC+ WLNEFSEYHNE L +ELKQIRA YP + +IYADY+N
Sbjct: 241 IGCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQIRALYPRIRIIYADYYN 300

Query: 301 SAMRFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHL 360
           SAM F++APQDFG TNTLQ CLV + +    H  YG     + ++C +PS+YV WDG+HL
Sbjct: 301 SAMLFYDAPQDFGLTNTLQICLVNDKEALNTHRKYG-----YSSLCDNPSEYVSWDGMHL 360

Query: 361 TEAAYRAIAIGLLQGPYTIPQITTSCISQNATTNLPQ 392
           TEAAY  IAI LL+GPYT PQI TSCIS NATT L Q
Sbjct: 361 TEAAYEVIAIALLEGPYTTPQIKTSCISHNATTTLLQ 391

BLAST of MC01g1485 vs. NCBI nr
Match: XP_008439763.1 (PREDICTED: GDSL esterase/lipase At1g28570-like [Cucumis melo])

HSP 1 Score: 507 bits (1306), Expect = 1.55e-176
Identity = 259/401 (64.59%), Postives = 316/401 (78.80%), Query Frame = 0

Query: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCG--YSSIFNFGDSLSDTGNLFDECSSGE 60
           MDSSN S    LIIF +A     A +P  +  G  ++SIFNFGDSLSDTGNLF+ C+S +
Sbjct: 1   MDSSNLSLNRWLIIFFVAT----ALSPSTNVLGNCFNSIFNFGDSLSDTGNLFNNCNSNK 60

Query: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120
           P KSCF PYG+TFFR PTGR SDGRLI+DFIA+SLGLPL+QPYLGV  + ++  +FEKGL
Sbjct: 61  PPKSCFTPYGDTFFRHPTGRFSDGRLIIDFIAQSLGLPLLQPYLGVETQRMSVAEFEKGL 120

Query: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSL 180
           NFAVAGATALDAS+LRE    +V TNYSL+VQLEWF+ AYS  C SSS+ RC +ILK SL
Sbjct: 121 NFAVAGATALDASYLREKAFVEVPTNYSLSVQLEWFKKAYSLACPSSSSTRCTKILKKSL 180

Query: 181 VVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGC 240
            VVGEIGGNDYN PFF++ SFE+IKSLVP+VVK I S I ELI+LGA++L+VPGNLPIGC
Sbjct: 181 FVVGEIGGNDYNYPFFKQHSFEEIKSLVPVVVKSIGSAITELIQLGAQSLIVPGNLPIGC 240

Query: 241 NPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAM 300
            P YLK+Y TS+++S+NGCL+WLN+FSEYHN+ L+EEL +IR+ +P+V +IYADYHNSAM
Sbjct: 241 CPKYLKIYSTSIQDSKNGCLDWLNQFSEYHNKHLQEELNRIRSQHPNVQIIYADYHNSAM 300

Query: 301 RFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGI-----LPPKFKTVCADPSKYVCWDGI 360
           +F+N P++FG  NTL++CLV +++T KK  NYG+        K K  C DPSKYV WDG+
Sbjct: 301 QFYNHPENFGLANTLEACLVDKNETLKKDGNYGLRSKAKTKTKTKKECDDPSKYVSWDGV 360

Query: 361 HLTEAAYRAIAIGLLQGPYTIPQITTSCI-SQNATTNLPQL 393
           HLTEAAYR IA  +LQGPYT P+ TTSCI S N  TNL QL
Sbjct: 361 HLTEAAYRLIANAILQGPYTHPKFTTSCIMSHNLPTNLLQL 397

BLAST of MC01g1485 vs. ExPASy TrEMBL
Match: A0A6J1CMY9 (GDSL esterase/lipase At1g28600 OS=Momordica charantia OX=3673 GN=LOC111012455 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 5.60e-289
Identity = 393/394 (99.75%), Postives = 394/394 (100.00%), Query Frame = 0

Query: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH 60
           MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH
Sbjct: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH 60

Query: 61  KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF 120
           KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF
Sbjct: 61  KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF 120

Query: 121 AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVV 180
           AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLV+
Sbjct: 121 AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVL 180

Query: 181 VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP 240
           VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP
Sbjct: 181 VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP 240

Query: 241 TYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRF 300
           TYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRF
Sbjct: 241 TYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRF 300

Query: 301 FNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAY 360
           FNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAY
Sbjct: 301 FNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAY 360

Query: 361 RAIAIGLLQGPYTIPQITTSCISQNATTNLPQLL 394
           RAIAIGLLQGPYTIPQITTSCISQNATTNLPQLL
Sbjct: 361 RAIAIGLLQGPYTIPQITTSCISQNATTNLPQLL 394

BLAST of MC01g1485 vs. ExPASy TrEMBL
Match: A0A6J1EJ98 (GDSL esterase/lipase At1g28590-like OS=Cucurbita moschata OX=3662 GN=LOC111433109 PE=3 SV=1)

HSP 1 Score: 512 bits (1318), Expect = 9.42e-179
Identity = 268/397 (67.51%), Postives = 312/397 (78.59%), Query Frame = 0

Query: 1   MDSSNSSSIISLI-IFSIAVSSAFAFAPR-ASGCGYSSIFNFGDSLSDTGNLFDECSSGE 60
           M SSNSS + SL  +F + V S   FAP    GC ++SIFNFGDSLSDTGNLF  C S E
Sbjct: 1   MGSSNSSPVQSLNRLFIVLVLSLLTFAPPPVLGC-FTSIFNFGDSLSDTGNLFGNCVSEE 60

Query: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120
           P  SCF PYG+ FF  PTGR S+GRLI+DFIA+SLGLPL+ PYL V R+ I+ +DFEKGL
Sbjct: 61  PPHSCFPPYGDAFFHHPTGRFSNGRLIVDFIAQSLGLPLLPPYLSVGRRRISIQDFEKGL 120

Query: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTA--RCREILKS 180
           NFAVAGATALD S LRE     + TNYSL VQ+EWF++ YSSVC+SSSTA  +CR IL+ 
Sbjct: 121 NFAVAGATALDESVLREKGITNLPTNYSLGVQMEWFKNTYSSVCKSSSTATAKCRAILER 180

Query: 181 SLVVVGEIGGNDYNDPFF-ERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLP 240
           SL+VVGEIGGNDYN  F  ER++ ++IKSLV  VVK I S I ELIKLGA+TLMVPGNLP
Sbjct: 181 SLIVVGEIGGNDYNYAFIDERQNIDEIKSLVTPVVKAIGSAITELIKLGAQTLMVPGNLP 240

Query: 241 IGCNPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHN 300
           IGCNPTYLK+YGTSV+NSENGC+ WLNEFSEYHNE L +ELKQIRA YPH+ +IYADY+N
Sbjct: 241 IGCNPTYLKVYGTSVQNSENGCVNWLNEFSEYHNEQLLQELKQIRALYPHIRIIYADYYN 300

Query: 301 SAMRFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHL 360
           SAM F++APQDFG TNTLQ CLV E +TS  H  YG     + ++C +PS+YV WDG+HL
Sbjct: 301 SAMLFYDAPQDFGLTNTLQLCLVNEKETSNTHRKYG-----YSSLCDNPSEYVSWDGMHL 360

Query: 361 TEAAYRAIAIGLLQGPYTIPQITTSCISQNATTNLPQ 392
           TEAAY+ IAI LL+GPYT PQI TSCISQNATT L Q
Sbjct: 361 TEAAYKVIAIALLEGPYTTPQIKTSCISQNATTTLLQ 391

BLAST of MC01g1485 vs. ExPASy TrEMBL
Match: A0A1S3AZH8 (GDSL esterase/lipase At1g28570-like OS=Cucumis melo OX=3656 GN=LOC103484464 PE=3 SV=1)

HSP 1 Score: 507 bits (1306), Expect = 7.50e-177
Identity = 259/401 (64.59%), Postives = 316/401 (78.80%), Query Frame = 0

Query: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCG--YSSIFNFGDSLSDTGNLFDECSSGE 60
           MDSSN S    LIIF +A     A +P  +  G  ++SIFNFGDSLSDTGNLF+ C+S +
Sbjct: 1   MDSSNLSLNRWLIIFFVAT----ALSPSTNVLGNCFNSIFNFGDSLSDTGNLFNNCNSNK 60

Query: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120
           P KSCF PYG+TFFR PTGR SDGRLI+DFIA+SLGLPL+QPYLGV  + ++  +FEKGL
Sbjct: 61  PPKSCFTPYGDTFFRHPTGRFSDGRLIIDFIAQSLGLPLLQPYLGVETQRMSVAEFEKGL 120

Query: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSL 180
           NFAVAGATALDAS+LRE    +V TNYSL+VQLEWF+ AYS  C SSS+ RC +ILK SL
Sbjct: 121 NFAVAGATALDASYLREKAFVEVPTNYSLSVQLEWFKKAYSLACPSSSSTRCTKILKKSL 180

Query: 181 VVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGC 240
            VVGEIGGNDYN PFF++ SFE+IKSLVP+VVK I S I ELI+LGA++L+VPGNLPIGC
Sbjct: 181 FVVGEIGGNDYNYPFFKQHSFEEIKSLVPVVVKSIGSAITELIQLGAQSLIVPGNLPIGC 240

Query: 241 NPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAM 300
            P YLK+Y TS+++S+NGCL+WLN+FSEYHN+ L+EEL +IR+ +P+V +IYADYHNSAM
Sbjct: 241 CPKYLKIYSTSIQDSKNGCLDWLNQFSEYHNKHLQEELNRIRSQHPNVQIIYADYHNSAM 300

Query: 301 RFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGI-----LPPKFKTVCADPSKYVCWDGI 360
           +F+N P++FG  NTL++CLV +++T KK  NYG+        K K  C DPSKYV WDG+
Sbjct: 301 QFYNHPENFGLANTLEACLVDKNETLKKDGNYGLRSKAKTKTKTKKECDDPSKYVSWDGV 360

Query: 361 HLTEAAYRAIAIGLLQGPYTIPQITTSCI-SQNATTNLPQL 393
           HLTEAAYR IA  +LQGPYT P+ TTSCI S N  TNL QL
Sbjct: 361 HLTEAAYRLIANAILQGPYTHPKFTTSCIMSHNLPTNLLQL 397

BLAST of MC01g1485 vs. ExPASy TrEMBL
Match: A0A6J1IR01 (GDSL esterase/lipase At1g28570-like OS=Cucurbita maxima OX=3661 GN=LOC111478589 PE=3 SV=1)

HSP 1 Score: 506 bits (1304), Expect = 1.26e-176
Identity = 265/397 (66.75%), Postives = 309/397 (77.83%), Query Frame = 0

Query: 1   MDSSNSSSIISLI-IFSIAVSSAFAFAPR-ASGCGYSSIFNFGDSLSDTGNLFDECSSGE 60
           M SSNSS ++SL  +F I V     FAP    GC ++SIFNFGDSLSDTGNLF +C S E
Sbjct: 1   MGSSNSSPVLSLNRLFIILVLPLLTFAPPPVLGC-FTSIFNFGDSLSDTGNLFGKCVSEE 60

Query: 61  PHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGL 120
           P  SCF PYG+ FF  PTGR S+GRLI+DFIA+SLGLPL+ PYL V R+ I  EDFEKGL
Sbjct: 61  PPHSCFPPYGDAFFHHPTGRFSNGRLIIDFIAQSLGLPLLPPYLSVGRRRINIEDFEKGL 120

Query: 121 NFAVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTA--RCREILKS 180
           NFAVAGATALD + LRE     + TNYSL VQLEWF++ YSSVC+SSSTA  +CR ILKS
Sbjct: 121 NFAVAGATALDEAVLREKGITNLSTNYSLGVQLEWFKNTYSSVCKSSSTATAKCRAILKS 180

Query: 181 SLVVVGEIGGNDYNDPFF-ERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLP 240
           SL+VVGEIGGNDYN  F  ER++ E+IKSLVP VVK I S I ELIKLGA+TLMVPGNLP
Sbjct: 181 SLIVVGEIGGNDYNYAFIDERQNIEEIKSLVPPVVKAIGSAITELIKLGAQTLMVPGNLP 240

Query: 241 IGCNPTYLKLYGTSVKNSENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHN 300
           IGCNPTYLK+YGTSV+N ENGC+ WLNEFS+YHNE L EELKQIRA YPH+ +IYADY+N
Sbjct: 241 IGCNPTYLKVYGTSVQNPENGCVNWLNEFSQYHNEQLLEELKQIRALYPHIRIIYADYYN 300

Query: 301 SAMRFFNAPQDFGFTNTLQSCLVLEDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHL 360
           SAM F++AP+DFG TNTLQ CLV E++T      YG     + ++C +PS+YV WDG+HL
Sbjct: 301 SAMLFYDAPRDFGLTNTLQICLVNEEETLNTDRKYG-----YSSLCDNPSEYVSWDGMHL 360

Query: 361 TEAAYRAIAIGLLQGPYTIPQITTSCISQNATTNLPQ 392
           TEAAY+ IAI  L+GPYT P+I TSCISQN TT   Q
Sbjct: 361 TEAAYKVIAIAWLEGPYTTPRIKTSCISQNVTTTFIQ 391

BLAST of MC01g1485 vs. ExPASy TrEMBL
Match: A0A0A0KHU0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G519430 PE=3 SV=1)

HSP 1 Score: 414 bits (1064), Expect = 3.37e-141
Identity = 205/312 (65.71%), Postives = 252/312 (80.77%), Query Frame = 0

Query: 86  LDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNFAVAGATALDASFLRENMSCKVHTNY 145
           L F A+SLG+PL+QPYLGV  + ++ ++FEKGLNFAV GATAL+AS+LRE +  +V TNY
Sbjct: 14  LLFTAQSLGIPLLQPYLGVETQRMSIDEFEKGLNFAVGGATALNASYLREKVFVEVPTNY 73

Query: 146 SLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVVVGEIGGNDYNDPFFERRSFEDIKSL 205
           SL+VQLEWFR AYS  C SSS+ RC EILK SL VVGEIGGNDYN PFF++ SFE+IKSL
Sbjct: 74  SLSVQLEWFRKAYSLACPSSSSTRCTEILKKSLFVVGEIGGNDYNYPFFKQHSFEEIKSL 133

Query: 206 VPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNPTYLKLYGTSVKNSENGCLEWLNEFS 265
           VPLVVK I STI ELI LGA++L+VPGNLPIGC+  YL++Y TS+++S+NGCL+WLN+FS
Sbjct: 134 VPLVVKSIGSTITELIHLGAQSLLVPGNLPIGCSSKYLQIYSTSIQDSKNGCLDWLNQFS 193

Query: 266 EYHNELLKEELKQIRANYPHVHLIYADYHNSAMRFFNAPQDFGFTNTLQSCLVLEDQTSK 325
           EYHN+ L+EEL +IR+ +P+V +IYADYHNSAM+F+N P++FG  NTL++CLV  ++T K
Sbjct: 194 EYHNKYLQEELNRIRSRHPNVQIIYADYHNSAMQFYNHPENFGLKNTLEACLVDRNETLK 253

Query: 326 KHTNYGI---LPPKFKTVCADPSKYVCWDGIHLTEAAYRAIAIGLLQGPYTIPQITTSCI 385
           K   YG+      K K  C DPSKYV WDG+HLTEAAYR IA+GLLQGPYT PQ TTSCI
Sbjct: 254 KDGKYGLGGKTKTKTKIECDDPSKYVSWDGVHLTEAAYRLIAMGLLQGPYTHPQFTTSCI 313

Query: 386 -SQNATTNLPQL 393
            S N  TNL QL
Sbjct: 314 ISHNLPTNLFQL 325

BLAST of MC01g1485 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 2.0e-92
Identity = 177/384 (46.09%), Postives = 245/384 (63.80%), Query Frame = 0

Query: 7   SSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPHKSCFAP 66
           +S+ SL+IF  +       +       + SI +FGDS++DTGNL       +   + F P
Sbjct: 2   ASLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFPP 61

Query: 67  YGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNFAVAGAT 126
           YGETFF  PTGR  DGR+I+DFIAE +GLP V PY G + +     +F+KG+NFAVAGAT
Sbjct: 62  YGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNR-----NFDKGVNFAVAGAT 121

Query: 127 ALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVVVGEIGG 186
           AL +SFL++    + HTN SL VQL+ F+ +  ++C S S   CR+++ ++L+++GEIGG
Sbjct: 122 ALKSSFLKKR-GIQPHTNVSLGVQLKSFKKSLPNLCGSPSD--CRDMIGNALILMGEIGG 181

Query: 187 NDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNPTYLKLY 246
           NDYN PFF R+  ++++ LVP V+  I+STI ELI +G +T +VPG  PIGC+  YL LY
Sbjct: 182 NDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLY 241

Query: 247 GTSVKNS---ENGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRFFNA 306
            TS K+      GCL+WLN+F EYH+E LK EL ++R  YPHV++IYADY+NS +R F  
Sbjct: 242 KTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKE 301

Query: 307 PQDFGF-TNTLQSCLVL----EDQTSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTEA 366
           P  FGF      +C  +        ++K  + G+        C DPSKYV WDG+H+TEA
Sbjct: 302 PAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGV------KSCKDPSKYVGWDGVHMTEA 361

Query: 367 AYRAIAIGLLQGPYTIPQITTSCI 383
           AY+ IA G+L GPY  P    SC+
Sbjct: 362 AYKWIADGILNGPYANPPFDRSCL 371

BLAST of MC01g1485 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.6e-92
Identity = 178/357 (49.86%), Postives = 236/357 (66.11%), Query Frame = 0

Query: 34  YSSIFNFGDSLSDTGNLFDECSSGEPHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESL 93
           + SI +FGDS++DTGNL          K  F PYGETFF  PTGR S+GRLI+DFIAE L
Sbjct: 32  FKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGETFFHHPTGRFSNGRLIIDFIAEFL 91

Query: 94  GLPLVQPYLGVRRKGITAEDFEKGLNFAVAGATALDASFLRENMSCKVHTNYSLAVQLEW 153
           G PLV P+ G +       +FEKG+NFAV GATAL+ SFL E      +TN SLAVQL  
Sbjct: 92  GFPLVPPFYGSQN-----ANFEKGVNFAVGGATALERSFLEERGIHFPYTNVSLAVQLSS 151

Query: 154 FRDAYSSVCRSSSTARCREILKSSLVVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQI 213
           F+++  ++C S S   CR+++++SL+++GEIGGNDYN  FF  ++ E+IK LVPLV++ I
Sbjct: 152 FKESLPNLCVSPSD--CRDMIENSLILMGEIGGNDYNYAFFVGKNIEEIKELVPLVIETI 211

Query: 214 ASTIIELIKLGAETLMVPGNLPIGCNPTYLKLYGTSVKNSE-----NGCLEWLNEFSEYH 273
           +S I ELI +G +T +VPG  P+GC+  YL LY TS  N E      GCL+WLN+FSEYH
Sbjct: 212 SSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTS--NIEEYDPLTGCLKWLNKFSEYH 271

Query: 274 NELLKEELKQIRANYPHVHLIYADYHNSAMRFFNAPQDFGF-TNTLQSCLVLEDQTS-KK 333
           +E L+ EL +++  YPHV++IYADY+N+ +R    P  FGF +  L +C  L    +   
Sbjct: 272 DEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRPLPACCALGGPFNFTL 331

Query: 334 HTNYGILPPKFKTVCADPSKYVCWDGIHLTEAAYRAIAIGLLQGPYTIPQITTSCIS 384
               G   P+    C DPSKYV WDG+H+TEAAYR +A G+L+GPY IP    SC+S
Sbjct: 332 GRKRGTQVPE---CCDDPSKYVSWDGVHMTEAAYRLMAEGILKGPYAIPPFDWSCLS 376

BLAST of MC01g1485 vs. TAIR 10
Match: AT2G27360.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-92
Identity = 173/361 (47.92%), Postives = 234/361 (64.82%), Query Frame = 0

Query: 34  YSSIFNFGDSLSDTGNLFDECSSGEPHKSCFAPYGETFFRRPTGRCSDGRLILDFIAESL 93
           + SI +FGDS++DTGNL    S  +  +S F PYGETFF  P+GR SDGRLI+DFIAE L
Sbjct: 31  FKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETFFHHPSGRFSDGRLIIDFIAEFL 90

Query: 94  GLPLVQPYLGVRRKGITAEDFEKGLNFAVAGATALDASFLRENMSCKVHTNYSLAVQLEW 153
           G+P V P+ G +       +FEKG+NFAV GATAL+ S L E  +    +N SL  QL+ 
Sbjct: 91  GIPHVPPFYGSKN-----GNFEKGVNFAVGGATALECSVLEEKGTHCSQSNISLGNQLKS 150

Query: 154 FRDAYSSVCRSSSTARCREILKSSLVVVGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQI 213
           F+++   +C SSS   CR++++++ +++GEIGGNDYN P F+R++ E++K LVPLV+  I
Sbjct: 151 FKESLPYLCGSSS-PDCRDMIENAFILIGEIGGNDYNFPLFDRKNIEEVKELVPLVITTI 210

Query: 214 ASTIIELIKLGAETLMVPGNLPIGCNPTYLKLYGTSVKNSEN---GCLEWLNEFSEYHNE 273
           +S I EL+ +GA T +VPGN P+GC+  YL LY T  K   N   GCL WLN+FS YHNE
Sbjct: 211 SSAISELVDMGARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLTWLNDFSVYHNE 270

Query: 274 LLKEELKQIRANYPHVHLIYADYHNSAMRFFNAPQDFGFTN-TLQSCLVLEDQTSKKHTN 333
            L+ ELK++R  YPHV++IY DY+N+ +R    P  FG  +  L +C  L       +  
Sbjct: 271 QLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQEPSKFGLMDRPLPACCGL---GGPYNFT 330

Query: 334 YGI-LPPKFKTVCADPSKYVCWDGIHLTEAAYRAIAIGLLQGPYTIPQITTSCISQNATT 390
           + I    K    C+DPSKYV WDGIH+TEAAY+ I+ G+L GPY IP    SC+      
Sbjct: 331 FSIKCGSKGVEYCSDPSKYVNWDGIHMTEAAYKWISEGVLTGPYAIPPFNWSCLDSKIKN 382

BLAST of MC01g1485 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-90
Identity = 179/391 (45.78%), Postives = 252/391 (64.45%), Query Frame = 0

Query: 1   MDSSNSSSIISLIIFSIAVSSAFAFAPRASGCGYSSIFNFGDSLSDTGNLFDECSSGEPH 60
           +DS  +  ++  I+ ++ V+S  +   +     + SI +FGDS++DTGNL       +  
Sbjct: 4   LDSLPAMKLVRFILSTLLVTSVNS---QTQCRNFKSIISFGDSIADTGNLLGLSDPNDLP 63

Query: 61  KSCFAPYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNF 120
            S F PYGETFF  PTGR SDGRLI+DFIAE LG PLV P+ G +       +F+KG+NF
Sbjct: 64  ASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQN-----ANFKKGVNF 123

Query: 121 AVAGATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVV 180
           AVAGATAL+ SFL E       TN SL+VQL  F ++  ++C S S   CR++++++L++
Sbjct: 124 AVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGSPSD--CRDMIENALIL 183

Query: 181 VGEIGGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNP 240
           +GEIGGNDYN   F+R+  ++++ LVP V+  I+S I EL+ +G  T +VPGN PIG + 
Sbjct: 184 MGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSA 243

Query: 241 TYLKLYGTSVKNSEN---GCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSA 300
           +YL LY TS K   +   GCL+WLN+FSEY+N+ L+EEL  +R  YPHV++IYADY+N+ 
Sbjct: 244 SYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNAL 303

Query: 301 MRFFNAPQDFGFTN-TLQSCLVL----EDQTSKKHTNYGILPPKFKTVCADPSKYVCWDG 360
           +R F  P  FGF N  L +C  +        S++  + G+        C DPS+YV +DG
Sbjct: 304 LRLFQEPAKFGFMNRPLPACCGVGGSYNFNFSRRCGSVGV------EYCDDPSQYVNYDG 363

Query: 361 IHLTEAAYRAIAIGLLQGPYTIPQITTSCIS 384
           IH+TEAAYR I+ GLL+GPY IP    SC+S
Sbjct: 364 IHMTEAAYRLISEGLLKGPYAIPPFKWSCLS 378

BLAST of MC01g1485 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.2e-89
Identity = 180/385 (46.75%), Postives = 241/385 (62.60%), Query Frame = 0

Query: 6   SSSIISLIIFSIAVSSAFAFAPRASGC-GYSSIFNFGDSLSDTGNLFDECSSGEPHKSCF 65
           +S I+S ++  I   +    A   S C  Y SI +FGDS++DTGN     +     ++ F
Sbjct: 8   ASLIVSFLL--ILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAF 67

Query: 66  APYGETFFRRPTGRCSDGRLILDFIAESLGLPLVQPYLGVRRKGITAEDFEKGLNFAVAG 125
            PYGE+FF  P+GR SDGRL++DFIAE LGLP V PY G +        F +G+NFAV G
Sbjct: 68  LPYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPYFGSQN-----VSFNQGINFAVYG 127

Query: 126 ATALDASFLRENMSCKVHTNYSLAVQLEWFRDAYSSVCRSSSTARCREILKSSLVVVGEI 185
           ATALD +FL +       TN SL+VQL  F+    ++C +SST  CRE+L  SL+++GEI
Sbjct: 128 ATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPNLC-ASSTRDCREMLGDSLILMGEI 187

Query: 186 GGNDYNDPFFERRSFEDIKSLVPLVVKQIASTIIELIKLGAETLMVPGNLPIGCNPTYLK 245
           GGNDYN PFFE +S  +IK LVPL++K I+S I++LI LG +T +VPGN PIGC+  YL 
Sbjct: 188 GGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLT 247

Query: 246 LYGTSVKNSE--NGCLEWLNEFSEYHNELLKEELKQIRANYPHVHLIYADYHNSAMRFFN 305
           L+ T+    +   GC+ WLN+F E+HNE LK ELKQ++  YPHV++IYADY+NS    F 
Sbjct: 248 LFQTATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQ 307

Query: 306 APQDFGFTN-TLQSCLVLEDQ----TSKKHTNYGILPPKFKTVCADPSKYVCWDGIHLTE 365
            P  +GF N  L +C  +  Q      K+    G+      + C +PS+YV WDG HLTE
Sbjct: 308 EPAKYGFKNRPLAACCGVGGQYNFTIGKECGENGV------SYCQNPSEYVNWDGYHLTE 367

Query: 366 AAYRAIAIGLLQGPYTIPQITTSCI 383
           A Y+ +A GLL G YT P    SC+
Sbjct: 368 ATYQKMAQGLLNGRYTTPAFDWSCL 378