MC01g1345 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g1345
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMagnesium transporter protein 1
LocationMC01: 18118627 .. 18119670 (-)
RNA-Seq ExpressionMC01g1345
SyntenyMC01g1345
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACTTTTTTTAAGTTCCCTCATACTCCCCTCATCTTAGCTCTCCTTCTCACTCTCTTCGCCCAAAATTCCCTCTCCTCCTCCGACTCCGACTCCGACCAAGTTTCCGAGCTCCTCGACCTCCAATCCCGATCCCCATCTGGCGTAATCCATCTCAACGACCAGTCTGTTTCTCGTTTTCTCACGTCCCCCAAAAGCCCTAGACCCTATTCGCTCCTCATCTTCTTCGATGCTCTACAGCTTCACGACAAGTCGGAGCTCCACCTCAAAGAACTCCGCCGTGAATTTGGCCTCGTCTCCTCCTCCTTCATCGCCAACAACCCCGATCCATCTTCTCCTTCTCGCTCCAAACTATTCTTCTGCGAGATTGAATTCAAAGAGTCCCAGCCCAGTTTCTCTCTTTTCGGCGTCAATGCCCTCCCCCATGTCCGCCTTATTGGCACCAGCCAAACGCCCAAGCACTCCGATCAAATGGACCAGGGTGACTTTTCCAGATTGGCCGAATCCATGGCGGAGTTTGTAGAGTCCAAGACTTCGCTTGTCATTGGTCCGATCCATCGACCACCATTGTTTTCCAAGAATCAATTGGCTATTCTTATCATTGCGATGTTAATTTCCGCGCCATTTGCTTTGAGAAAGGTCATTGCGGGTGAGACGTTGCTCAACGACCGGAAGGTCTGGCTCGTTGGGGCTATCTTCATCTACTTTTTCAGTGTTGCGGGTACAATGCATAACATAATTCGGAAAATGCCCATGTTTTTGGCGGACCGCAACGATCCCTCCAAGCTGATATTCTTCTATCAGGGCTCCGGAATGCAGCTCGGTGCGGAGGGATTCGCTGTGGGATTCTTATATACGATTGTAGGGCTGCTTTTGGCTTTCATTACTCACGTGCTTGTCAAGGTCAGGAATGCGAATACTCAGCGGGTCGTTATGATGGTTGCCCTCTTTGTATCATTTTGGGCAGTGAAGAAAGTCGTATATTTGGATAACTGGAAGACAGGTTACGGAATTCATGGATATTGGCCATCAAGTTGGAAT

mRNA sequence

ATGGCGACTTTTTTTAAGTTCCCTCATACTCCCCTCATCTTAGCTCTCCTTCTCACTCTCTTCGCCCAAAATTCCCTCTCCTCCTCCGACTCCGACTCCGACCAAGTTTCCGAGCTCCTCGACCTCCAATCCCGATCCCCATCTGGCGTAATCCATCTCAACGACCAGTCTGTTTCTCGTTTTCTCACGTCCCCCAAAAGCCCTAGACCCTATTCGCTCCTCATCTTCTTCGATGCTCTACAGCTTCACGACAAGTCGGAGCTCCACCTCAAAGAACTCCGCCGTGAATTTGGCCTCGTCTCCTCCTCCTTCATCGCCAACAACCCCGATCCATCTTCTCCTTCTCGCTCCAAACTATTCTTCTGCGAGATTGAATTCAAAGAGTCCCAGCCCAGTTTCTCTCTTTTCGGCGTCAATGCCCTCCCCCATGTCCGCCTTATTGGCACCAGCCAAACGCCCAAGCACTCCGATCAAATGGACCAGGGTGACTTTTCCAGATTGGCCGAATCCATGGCGGAGTTTGTAGAGTCCAAGACTTCGCTTGTCATTGGTCCGATCCATCGACCACCATTGTTTTCCAAGAATCAATTGGCTATTCTTATCATTGCGATGTTAATTTCCGCGCCATTTGCTTTGAGAAAGGTCATTGCGGGTGAGACGTTGCTCAACGACCGGAAGGTCTGGCTCGTTGGGGCTATCTTCATCTACTTTTTCAGTGTTGCGGGTACAATGCATAACATAATTCGGAAAATGCCCATGTTTTTGGCGGACCGCAACGATCCCTCCAAGCTGATATTCTTCTATCAGGGCTCCGGAATGCAGCTCGGTGCGGAGGGATTCGCTGTGGGATTCTTATATACGATTGTAGGGCTGCTTTTGGCTTTCATTACTCACGTGCTTGTCAAGGTCAGGAATGCGAATACTCAGCGGGTCGTTATGATGGTTGCCCTCTTTGTATCATTTTGGGCAGTGAAGAAAGTCGTATATTTGGATAACTGGAAGACAGGTTACGGAATTCATGGATATTGGCCATCAAGTTGGAAT

Coding sequence (CDS)

ATGGCGACTTTTTTTAAGTTCCCTCATACTCCCCTCATCTTAGCTCTCCTTCTCACTCTCTTCGCCCAAAATTCCCTCTCCTCCTCCGACTCCGACTCCGACCAAGTTTCCGAGCTCCTCGACCTCCAATCCCGATCCCCATCTGGCGTAATCCATCTCAACGACCAGTCTGTTTCTCGTTTTCTCACGTCCCCCAAAAGCCCTAGACCCTATTCGCTCCTCATCTTCTTCGATGCTCTACAGCTTCACGACAAGTCGGAGCTCCACCTCAAAGAACTCCGCCGTGAATTTGGCCTCGTCTCCTCCTCCTTCATCGCCAACAACCCCGATCCATCTTCTCCTTCTCGCTCCAAACTATTCTTCTGCGAGATTGAATTCAAAGAGTCCCAGCCCAGTTTCTCTCTTTTCGGCGTCAATGCCCTCCCCCATGTCCGCCTTATTGGCACCAGCCAAACGCCCAAGCACTCCGATCAAATGGACCAGGGTGACTTTTCCAGATTGGCCGAATCCATGGCGGAGTTTGTAGAGTCCAAGACTTCGCTTGTCATTGGTCCGATCCATCGACCACCATTGTTTTCCAAGAATCAATTGGCTATTCTTATCATTGCGATGTTAATTTCCGCGCCATTTGCTTTGAGAAAGGTCATTGCGGGTGAGACGTTGCTCAACGACCGGAAGGTCTGGCTCGTTGGGGCTATCTTCATCTACTTTTTCAGTGTTGCGGGTACAATGCATAACATAATTCGGAAAATGCCCATGTTTTTGGCGGACCGCAACGATCCCTCCAAGCTGATATTCTTCTATCAGGGCTCCGGAATGCAGCTCGGTGCGGAGGGATTCGCTGTGGGATTCTTATATACGATTGTAGGGCTGCTTTTGGCTTTCATTACTCACGTGCTTGTCAAGGTCAGGAATGCGAATACTCAGCGGGTCGTTATGATGGTTGCCCTCTTTGTATCATTTTGGGCAGTGAAGAAAGTCGTATATTTGGATAACTGGAAGACAGGTTACGGAATTCATGGATATTGGCCATCAAGTTGGAAT

Protein sequence

MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIEFKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Homology
BLAST of MC01g1345 vs. ExPASy Swiss-Prot
Match: Q9SYB5 (Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B OS=Arabidopsis thaliana OX=3702 GN=OST3B PE=2 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 3.3e-128
Identity = 231/349 (66.19%), Postives = 282/349 (80.80%), Query Frame = 0

Query: 1   MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSR 60
           MA   K      ++A L + FA  S S SDSDSD ++EL+ L+S S SGVIHL+D  +S+
Sbjct: 1   MALKSKLVSLLFLIATLSSTFAA-SFSDSDSDSDLLNELVSLRSTSESGVIHLDDHGISK 60

Query: 61  FLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLF 120
           FLTS  +PRPYSLL+FFDA QLH K+EL L+ELRREFG+VS+SF+ANN   +    +KLF
Sbjct: 61  FLTSASTPRPYSLLVFFDATQLHSKNELRLQELRREFGIVSASFLANN---NGSEGTKLF 120

Query: 121 FCEIEFKESQPSFSLFGVNALPHVRLI--GTSQTPKHSDQMDQGDFSRLAESMAEFVESK 180
           FCEIEF +SQ SF LFGVNALPH+RL+    S     S QMDQ D+SRLAESMAEFVE +
Sbjct: 121 FCEIEFSKSQSSFQLFGVNALPHIRLVSPSISNLRDESGQMDQSDYSRLAESMAEFVEQR 180

Query: 181 TSLVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFF 240
           T L +GPI RPPL SK Q+ I++  ++I+ PF +++V+ GET+L+D ++WL GAIFIYFF
Sbjct: 181 TKLKVGPIQRPPLLSKPQIGIIVALIVIATPFIIKRVLKGETILHDTRLWLSGAIFIYFF 240

Query: 241 SVAGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITH 300
           SVAGTMHNIIRKMPMFL DRNDP+KL+FFYQGSGMQLGAEGFAVGFLYT+VGLLLAF+T+
Sbjct: 241 SVAGTMHNIIRKMPMFLQDRNDPNKLVFFYQGSGMQLGAEGFAVGFLYTVVGLLLAFVTN 300

Query: 301 VLVKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSW 348
           VLV+V+N   QR++M++ALF+SFWAVKKVVYLDNWKTGYGIH YWPSSW
Sbjct: 301 VLVRVKNITAQRLIMLLALFISFWAVKKVVYLDNWKTGYGIHPYWPSSW 345

BLAST of MC01g1345 vs. ExPASy Swiss-Prot
Match: Q94I55 (Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 OS=Oryza sativa subsp. japonica OX=39947 GN=OST3 PE=2 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 2.5e-112
Identity = 202/338 (59.76%), Postives = 264/338 (78.11%), Query Frame = 0

Query: 11  PLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSPKSPRP 70
           P  L LLLTL      +S+ +D D V+EL  L+SRSPSGVIHL D S++RFL++P +PRP
Sbjct: 5   PHPLLLLLTLLVAAGAASAGAD-DLVAELQSLRSRSPSGVIHLTDTSITRFLSAP-APRP 64

Query: 71  YSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIEFKESQ 130
           YS+L+FFDA  LH K++LHL +LRREF L+S+SF+A+N     P+ + LFF +IEF ESQ
Sbjct: 65  YSVLVFFDAASLHSKTDLHLPQLRREFALLSASFLAHN-----PASADLFFADIEFSESQ 124

Query: 131 PSFSLFGVNALPHVRLIGTSQTP-KHSDQMDQGDFSRLAESMAEFVESKTSLVIGPIHRP 190
            SF+ FGVN+LPHVRL+    T    S+QMDQ  FSRLA+SMAEFVES+T L +GPI RP
Sbjct: 125 HSFAQFGVNSLPHVRLVRPEHTRLAGSEQMDQSHFSRLADSMAEFVESRTGLEVGPIVRP 184

Query: 191 PLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMHNIIR 250
           PL S+NQ+ +L+I  L+S PF +++++ GETL +DR+VW+ GA+FIYFFSV+G M+ IIR
Sbjct: 185 PLVSRNQMILLVILFLVSIPFLIKRIMDGETLFHDRRVWMAGALFIYFFSVSGGMYGIIR 244

Query: 251 KMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRNANTQ 310
             PMF+ DR+DP+KL+FFYQGSGMQLGAEGFAVGFLYT+VGL++A +TH+LV+V +   Q
Sbjct: 245 HTPMFITDRSDPNKLVFFYQGSGMQLGAEGFAVGFLYTLVGLMIAMVTHLLVRVESLQIQ 304

Query: 311 RVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSW 348
           R  M+  + + +WAVKKV+ LDNWKTGY IH +WPSSW
Sbjct: 305 RFTMLAVMIIGWWAVKKVILLDNWKTGYSIHTFWPSSW 335

BLAST of MC01g1345 vs. ExPASy Swiss-Prot
Match: F4I8X8 (Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3A OS=Arabidopsis thaliana OX=3702 GN=OST3A PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.5e-104
Identity = 191/333 (57.36%), Postives = 254/333 (76.28%), Query Frame = 0

Query: 16  LLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSPKSPRPYSLLI 75
           L++ LF   +L++  SDSD  +EL+ L+S + SGVI  N+  VS+F+TS  +PRPYSL+I
Sbjct: 14  LIVFLF---TLANPKSDSDLKNELVSLRSTAESGVISFNNDDVSKFITSVSTPRPYSLII 73

Query: 76  FFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIEFKESQPSFSL 135
           FFDA+ LH  S+L L E RREF LVS++FI NN + S  + +KLFFCEIE   S+ SF  
Sbjct: 74  FFDAVHLHGNSQLRLPEFRREFRLVSATFITNNNNES--NGTKLFFCEIESTHSEASFRR 133

Query: 136 FGVNALPHVRLIG-TSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGPIHRPPLFSK 195
           F V +LPH+ L+  T++    SDQMD GDF+ LAESMAEFVE +T L +  I RPPL SK
Sbjct: 134 FAVESLPHISLVSPTTENLTESDQMDGGDFTGLAESMAEFVERQTKLTVCSIQRPPLISK 193

Query: 196 NQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMHNIIRKMPMF 255
            Q+ I++  ++IS P  ++K++ GETLL+D ++WLVGA+F+YFFSV+GTMHNIIR+MPM+
Sbjct: 194 TQIGIIVAIIIISTPILIKKILKGETLLHDHRIWLVGAVFVYFFSVSGTMHNIIREMPMY 253

Query: 256 LADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRNANTQRVVMM 315
           + D  D SK +FF + S MQLGAEGF VGFLYT+VGLLLAF+T+V+V+V+  + QR+ M+
Sbjct: 254 IKDYEDSSKFVFFIEESEMQLGAEGFFVGFLYTVVGLLLAFVTNVVVRVKKLDEQRMAML 313

Query: 316 VALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSW 348
           +AL +SFWAV+KVVYLDNWKTGY I+ YWPSSW
Sbjct: 314 LALSISFWAVRKVVYLDNWKTGYEIYPYWPSSW 341

BLAST of MC01g1345 vs. ExPASy Swiss-Prot
Match: Q13454 (Tumor suppressor candidate 3 OS=Homo sapiens OX=9606 GN=TUSC3 PE=1 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-11
Identity = 69/307 (22.48%), Postives = 134/307 (43.65%), Query Frame = 0

Query: 12  LILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSPKSPRPY 71
           L+L LL            +  +++V +L++  SR    +  +N     +F+ +P  PR Y
Sbjct: 29  LLLLLLCIQLGGGQKKKENLLAEKVEQLMEWSSR--RSIFRMNGDKFRKFIKAP--PRNY 88

Query: 72  SLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIEFKESQP 131
           S+++ F ALQ   +  +  ++   E+       +AN+   SS   +KLFF  +++ E   
Sbjct: 89  SMIVMFTALQPQRQCSV-CRQANEEY-----QILANSWRYSSAFCNKLFFSMVDYDEGTD 148

Query: 132 SFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGPIHRPPL 191
            F    +N+ P          PK +D  D       AE +A+++  +T + I  + RPP 
Sbjct: 149 VFQQLNMNSAPTFMHFPPKGRPKRADTFDLQRIGFAAEQLAKWIADRTDVHI-RVFRPPN 208

Query: 192 FSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMHNIIRKM 251
           +S   +A+ ++  L+     LR+       + ++  W + ++ I F   +G M N IR  
Sbjct: 209 YS-GTIALALLVSLVGGLLYLRR--NNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGP 268

Query: 252 PMFLADRNDPSKLIFFYQGSGM-QLGAEGFAVGFLYTIVGLLLAFITHVLVKVRNANTQR 311
           P   A +N  +  + +  GS   Q  AE   +  L   + + +  +        +   +R
Sbjct: 269 P--YAHKNPHNGQVSYIHGSSQAQFVAESHIILVLNAAITMGMVLLNEAATSKGDVGKRR 319

Query: 312 VVMMVAL 318
           ++ +V L
Sbjct: 329 IICLVGL 319

BLAST of MC01g1345 vs. ExPASy Swiss-Prot
Match: Q8BTV1 (Tumor suppressor candidate 3 OS=Mus musculus OX=10090 GN=Tusc3 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.3e-11
Identity = 69/307 (22.48%), Postives = 135/307 (43.97%), Query Frame = 0

Query: 12  LILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSPKSPRPY 71
           L+L LL            +  +++V +L++  SR    +  +N     +F+ +P  PR Y
Sbjct: 29  LLLLLLCIQLGGGQKKKENLLAEKVEQLMEWSSR--RSIFRMNGDKFRKFVKAP--PRNY 88

Query: 72  SLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIEFKESQP 131
           S+++ F ALQ   +  +  ++   E+       +AN+   SS   +KLFF  +++ E   
Sbjct: 89  SMIVMFTALQPQRQCSV-CRQANEEY-----QILANSWRYSSAFCNKLFFGMVDYDEGTD 148

Query: 132 SFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGPIHRPPL 191
            F    +N+ P      +   PK +D  D       AE +A+++  +T + I  + RPP 
Sbjct: 149 VFQQLNMNSAPTFMHFPSKGRPKRADTFDLQRIGFAAEQLAKWIADRTDVHI-RVFRPPN 208

Query: 192 FSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMHNIIRKM 251
           +S   +A+ ++  L+     LR+       + ++  W + ++ I F   +G M N IR  
Sbjct: 209 YS-GTIALALLVSLVGGLLYLRR--NNLEFIYNKTGWAMVSLCIVFAMTSGQMWNHIRGP 268

Query: 252 PMFLADRNDPSKLIFFYQGSGM-QLGAEGFAVGFLYTIVGLLLAFITHVLVKVRNANTQR 311
           P   A +N  +  + +  GS   Q  AE   +  L   + + +  +        +   +R
Sbjct: 269 P--YAHKNPHNGQVSYIHGSSQAQFVAESHIILVLNAAITMGMVLLNEAATSKGDVGKRR 319

Query: 312 VVMMVAL 318
           ++ +V L
Sbjct: 329 IICLVGL 319

BLAST of MC01g1345 vs. NCBI nr
Match: XP_038881671.1 (probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [Benincasa hispida])

HSP 1 Score: 614 bits (1583), Expect = 2.60e-220
Identity = 315/348 (90.52%), Postives = 330/348 (94.83%), Query Frame = 0

Query: 1   MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSR 60
           MAT FKF H+ L+L LLLTLF Q SLSS  SDSD+VSELL LQSRSPSGVIHL+DQSVSR
Sbjct: 1   MATLFKFLHS-LLLTLLLTLFVQYSLSS--SDSDRVSELLALQSRSPSGVIHLDDQSVSR 60

Query: 61  FLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLF 120
           FLT+PKSPRPYSLLIFFDA QLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLF
Sbjct: 61  FLTTPKSPRPYSLLIFFDAQQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLF 120

Query: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTS 180
           FCEIEFKESQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTS
Sbjct: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTS 180

Query: 181 LVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSV 240
           LVIGPIHRPP+FSKNQL +L+IAMLISAPFAL+K+IAGETLLNDRKVWL+GAIF+YFFSV
Sbjct: 181 LVIGPIHRPPMFSKNQLVVLVIAMLISAPFALKKIIAGETLLNDRKVWLMGAIFVYFFSV 240

Query: 241 AGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300
           AGTMHNIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL
Sbjct: 241 AGTMHNIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300

Query: 301 VKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
           V VRN NTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 301 VNVRNVNTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 345

BLAST of MC01g1345 vs. NCBI nr
Match: TYK13063.1 (putative dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [Cucumis melo var. makuwa])

HSP 1 Score: 608 bits (1567), Expect = 6.86e-218
Identity = 310/343 (90.38%), Postives = 325/343 (94.75%), Query Frame = 0

Query: 6   KFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSP 65
           KF H PL+L LLL LF Q SLSS  SDSD+V+ELLDLQSRSPSGVIHL+DQSVSRFLT+P
Sbjct: 5   KFSH-PLLLTLLLALFVQYSLSS--SDSDRVAELLDLQSRSPSGVIHLDDQSVSRFLTTP 64

Query: 66  KSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIE 125
           KSPRPYSLLIFFDALQLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLFFCEIE
Sbjct: 65  KSPRPYSLLIFFDALQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLFFCEIE 124

Query: 126 FKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGP 185
           FKESQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTSLV+GP
Sbjct: 125 FKESQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTSLVVGP 184

Query: 186 IHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMH 245
           I RPP+FSKNQL +L IAMLISAPFAL+KVIAGETLLNDRKVWL+GAIFIYFFSVAGTMH
Sbjct: 185 IQRPPMFSKNQLVVLFIAMLISAPFALKKVIAGETLLNDRKVWLMGAIFIYFFSVAGTMH 244

Query: 246 NIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRN 305
           NIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAF+THVLVKVRN
Sbjct: 245 NIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFMTHVLVKVRN 304

Query: 306 ANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
            NTQRV MM+ALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 305 VNTQRVAMMIALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 344

BLAST of MC01g1345 vs. NCBI nr
Match: XP_008439963.2 (PREDICTED: probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [Cucumis melo])

HSP 1 Score: 608 bits (1569), Expect = 1.43e-217
Identity = 310/343 (90.38%), Postives = 325/343 (94.75%), Query Frame = 0

Query: 6   KFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSP 65
           KF H PL+L LLL LF Q SLSS  SDSD+V+ELLDLQSRSPSGVIHL+DQSVSRFLT+P
Sbjct: 44  KFSH-PLLLTLLLALFVQYSLSS--SDSDRVAELLDLQSRSPSGVIHLDDQSVSRFLTTP 103

Query: 66  KSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIE 125
           KSPRPYSLLIFFDALQLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLFFCEIE
Sbjct: 104 KSPRPYSLLIFFDALQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLFFCEIE 163

Query: 126 FKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGP 185
           FKESQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTSLV+GP
Sbjct: 164 FKESQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTSLVVGP 223

Query: 186 IHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMH 245
           I RPP+FSKNQL +L IAMLISAPFAL+KVIAGETLLNDRKVWL+GAIFIYFFSVAGTMH
Sbjct: 224 IQRPPMFSKNQLVVLFIAMLISAPFALKKVIAGETLLNDRKVWLMGAIFIYFFSVAGTMH 283

Query: 246 NIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRN 305
           NIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAF+THVLVKVRN
Sbjct: 284 NIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFVTHVLVKVRN 343

Query: 306 ANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
            NTQRV MM+ALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 344 VNTQRVAMMIALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 383

BLAST of MC01g1345 vs. NCBI nr
Match: XP_022978593.1 (probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [Cucurbita maxima])

HSP 1 Score: 603 bits (1555), Expect = 4.79e-216
Identity = 309/348 (88.79%), Postives = 327/348 (93.97%), Query Frame = 0

Query: 1   MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSR 60
           MATF  FPH+ L+LALLLTL AQNS+SS  S  D V+EL+ LQSRS SGVIHL+DQSVSR
Sbjct: 1   MATFSSFPHS-LLLALLLTLVAQNSVSS--SGPDLVAELIALQSRSDSGVIHLDDQSVSR 60

Query: 61  FLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLF 120
           FL+SPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFI NNPDPSS SRSKLF
Sbjct: 61  FLSSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFITNNPDPSSSSRSKLF 120

Query: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTS 180
           FCEIEFKESQPSFSLFGVNALPHVRLIG +QTP  SDQMDQ D+SRLAESMAEFVESKTS
Sbjct: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGPNQTPNKSDQMDQSDYSRLAESMAEFVESKTS 180

Query: 181 LVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSV 240
           LV+GPIHRPP+FSKNQ+  L+IAMLISAPFAL+K+IAGETLLNDRKVWL+GAIFIYFFSV
Sbjct: 181 LVVGPIHRPPMFSKNQITFLVIAMLISAPFALKKIIAGETLLNDRKVWLIGAIFIYFFSV 240

Query: 241 AGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300
           AGTMHNIIRKMPMF+ DRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL
Sbjct: 241 AGTMHNIIRKMPMFMTDRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300

Query: 301 VKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
           VKVRN NTQRVVM+VALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 301 VKVRNVNTQRVVMLVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 345

BLAST of MC01g1345 vs. NCBI nr
Match: XP_004134751.1 (probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [Cucumis sativus] >KGN49129.1 hypothetical protein Csa_003136 [Cucumis sativus])

HSP 1 Score: 602 bits (1552), Expect = 1.32e-215
Identity = 307/343 (89.50%), Postives = 323/343 (94.17%), Query Frame = 0

Query: 6   KFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSP 65
            F H PL+L LLLTLF Q SLSS  SDSD+V+ELLDLQSRSPSGVIHL+DQSVSRFLT+P
Sbjct: 5   NFSH-PLLLTLLLTLFVQYSLSS--SDSDRVAELLDLQSRSPSGVIHLDDQSVSRFLTTP 64

Query: 66  KSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIE 125
           KSPRPYSLLIFFDALQLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLFFCEIE
Sbjct: 65  KSPRPYSLLIFFDALQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLFFCEIE 124

Query: 126 FKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGP 185
           FK+SQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTSLV+GP
Sbjct: 125 FKQSQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTSLVVGP 184

Query: 186 IHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMH 245
           I RPP+FSKNQL +L IAMLISAPFAL+KVIAGETLLNDRKVWL GAIFIYFFSVAGTMH
Sbjct: 185 IQRPPMFSKNQLVVLFIAMLISAPFALKKVIAGETLLNDRKVWLTGAIFIYFFSVAGTMH 244

Query: 246 NIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRN 305
           NIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAF+THVLVKVRN
Sbjct: 245 NIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFMTHVLVKVRN 304

Query: 306 ANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
            N QRV M++ALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 305 VNIQRVFMIIALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 344

BLAST of MC01g1345 vs. ExPASy TrEMBL
Match: A0A5D3CMB2 (Putative dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006650 PE=3 SV=1)

HSP 1 Score: 608 bits (1567), Expect = 3.32e-218
Identity = 310/343 (90.38%), Postives = 325/343 (94.75%), Query Frame = 0

Query: 6   KFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSP 65
           KF H PL+L LLL LF Q SLSS  SDSD+V+ELLDLQSRSPSGVIHL+DQSVSRFLT+P
Sbjct: 5   KFSH-PLLLTLLLALFVQYSLSS--SDSDRVAELLDLQSRSPSGVIHLDDQSVSRFLTTP 64

Query: 66  KSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIE 125
           KSPRPYSLLIFFDALQLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLFFCEIE
Sbjct: 65  KSPRPYSLLIFFDALQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLFFCEIE 124

Query: 126 FKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGP 185
           FKESQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTSLV+GP
Sbjct: 125 FKESQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTSLVVGP 184

Query: 186 IHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMH 245
           I RPP+FSKNQL +L IAMLISAPFAL+KVIAGETLLNDRKVWL+GAIFIYFFSVAGTMH
Sbjct: 185 IQRPPMFSKNQLVVLFIAMLISAPFALKKVIAGETLLNDRKVWLMGAIFIYFFSVAGTMH 244

Query: 246 NIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRN 305
           NIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAF+THVLVKVRN
Sbjct: 245 NIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFMTHVLVKVRN 304

Query: 306 ANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
            NTQRV MM+ALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 305 VNTQRVAMMIALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 344

BLAST of MC01g1345 vs. ExPASy TrEMBL
Match: A0A1S3B0R6 (probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B OS=Cucumis melo OX=3656 GN=LOC103484594 PE=3 SV=1)

HSP 1 Score: 608 bits (1569), Expect = 6.95e-218
Identity = 310/343 (90.38%), Postives = 325/343 (94.75%), Query Frame = 0

Query: 6   KFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSP 65
           KF H PL+L LLL LF Q SLSS  SDSD+V+ELLDLQSRSPSGVIHL+DQSVSRFLT+P
Sbjct: 44  KFSH-PLLLTLLLALFVQYSLSS--SDSDRVAELLDLQSRSPSGVIHLDDQSVSRFLTTP 103

Query: 66  KSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIE 125
           KSPRPYSLLIFFDALQLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLFFCEIE
Sbjct: 104 KSPRPYSLLIFFDALQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLFFCEIE 163

Query: 126 FKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGP 185
           FKESQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTSLV+GP
Sbjct: 164 FKESQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTSLVVGP 223

Query: 186 IHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMH 245
           I RPP+FSKNQL +L IAMLISAPFAL+KVIAGETLLNDRKVWL+GAIFIYFFSVAGTMH
Sbjct: 224 IQRPPMFSKNQLVVLFIAMLISAPFALKKVIAGETLLNDRKVWLMGAIFIYFFSVAGTMH 283

Query: 246 NIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRN 305
           NIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAF+THVLVKVRN
Sbjct: 284 NIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFVTHVLVKVRN 343

Query: 306 ANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
            NTQRV MM+ALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 344 VNTQRVAMMIALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 383

BLAST of MC01g1345 vs. ExPASy TrEMBL
Match: A0A6J1ITN1 (probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B OS=Cucurbita maxima OX=3661 GN=LOC111478529 PE=3 SV=1)

HSP 1 Score: 603 bits (1555), Expect = 2.32e-216
Identity = 309/348 (88.79%), Postives = 327/348 (93.97%), Query Frame = 0

Query: 1   MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSR 60
           MATF  FPH+ L+LALLLTL AQNS+SS  S  D V+EL+ LQSRS SGVIHL+DQSVSR
Sbjct: 1   MATFSSFPHS-LLLALLLTLVAQNSVSS--SGPDLVAELIALQSRSDSGVIHLDDQSVSR 60

Query: 61  FLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLF 120
           FL+SPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFI NNPDPSS SRSKLF
Sbjct: 61  FLSSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFITNNPDPSSSSRSKLF 120

Query: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTS 180
           FCEIEFKESQPSFSLFGVNALPHVRLIG +QTP  SDQMDQ D+SRLAESMAEFVESKTS
Sbjct: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGPNQTPNKSDQMDQSDYSRLAESMAEFVESKTS 180

Query: 181 LVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSV 240
           LV+GPIHRPP+FSKNQ+  L+IAMLISAPFAL+K+IAGETLLNDRKVWL+GAIFIYFFSV
Sbjct: 181 LVVGPIHRPPMFSKNQITFLVIAMLISAPFALKKIIAGETLLNDRKVWLIGAIFIYFFSV 240

Query: 241 AGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300
           AGTMHNIIRKMPMF+ DRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL
Sbjct: 241 AGTMHNIIRKMPMFMTDRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300

Query: 301 VKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
           VKVRN NTQRVVM+VALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 301 VKVRNVNTQRVVMLVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 345

BLAST of MC01g1345 vs. ExPASy TrEMBL
Match: A0A0A0KJY0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G514900 PE=3 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 6.40e-216
Identity = 307/343 (89.50%), Postives = 323/343 (94.17%), Query Frame = 0

Query: 6   KFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSP 65
            F H PL+L LLLTLF Q SLSS  SDSD+V+ELLDLQSRSPSGVIHL+DQSVSRFLT+P
Sbjct: 5   NFSH-PLLLTLLLTLFVQYSLSS--SDSDRVAELLDLQSRSPSGVIHLDDQSVSRFLTTP 64

Query: 66  KSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIE 125
           KSPRPYSLLIFFDALQLHDKSELHLKELR EFGLVSSSFIANNPDPSSPSRSKLFFCEIE
Sbjct: 65  KSPRPYSLLIFFDALQLHDKSELHLKELRHEFGLVSSSFIANNPDPSSPSRSKLFFCEIE 124

Query: 126 FKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGP 185
           FK+SQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTSLV+GP
Sbjct: 125 FKQSQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTSLVVGP 184

Query: 186 IHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMH 245
           I RPP+FSKNQL +L IAMLISAPFAL+KVIAGETLLNDRKVWL GAIFIYFFSVAGTMH
Sbjct: 185 IQRPPMFSKNQLVVLFIAMLISAPFALKKVIAGETLLNDRKVWLTGAIFIYFFSVAGTMH 244

Query: 246 NIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRN 305
           NIIRKMPMFL DRNDPSKL+FFYQGSGMQLGAEGFAVGFLYTIVGLLLAF+THVLVKVRN
Sbjct: 245 NIIRKMPMFLTDRNDPSKLVFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFMTHVLVKVRN 304

Query: 306 ANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
            N QRV M++ALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 305 VNIQRVFMIIALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 344

BLAST of MC01g1345 vs. ExPASy TrEMBL
Match: A0A6J1GE54 (probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B OS=Cucurbita moschata OX=3662 GN=LOC111453366 PE=3 SV=1)

HSP 1 Score: 601 bits (1550), Expect = 1.34e-215
Identity = 308/348 (88.51%), Postives = 327/348 (93.97%), Query Frame = 0

Query: 1   MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSR 60
           MATF  FPH+ L+LALLLT+ AQNS++S  S  D V+ELL LQSRS SGVIHL+DQSVSR
Sbjct: 1   MATFSSFPHS-LLLALLLTVVAQNSVAS--SGPDLVAELLALQSRSDSGVIHLDDQSVSR 60

Query: 61  FLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLF 120
           FL+SPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFI NNPDPSS SRSKLF
Sbjct: 61  FLSSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFITNNPDPSSSSRSKLF 120

Query: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGTSQTPKHSDQMDQGDFSRLAESMAEFVESKTS 180
           FCEIEFKESQPSFSLFGVNALPHVRLIG +QTPK SDQMDQ D+SRLAESMAEFVESKTS
Sbjct: 121 FCEIEFKESQPSFSLFGVNALPHVRLIGPNQTPKKSDQMDQSDYSRLAESMAEFVESKTS 180

Query: 181 LVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSV 240
           LV+GPIHRPP+FSKNQ+  L+IAMLI APFAL+K+IAGETLLNDRKVWL+GAIFIYFFSV
Sbjct: 181 LVVGPIHRPPMFSKNQITFLVIAMLILAPFALKKIIAGETLLNDRKVWLIGAIFIYFFSV 240

Query: 241 AGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300
           AGTMHNIIRKMPMF+ DRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL
Sbjct: 241 AGTMHNIIRKMPMFMTDRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVL 300

Query: 301 VKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 348
           VKVRN NTQRVVM+VALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN
Sbjct: 301 VKVRNVNTQRVVMLVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSWN 345

BLAST of MC01g1345 vs. TAIR 10
Match: AT1G61790.1 (Oligosaccharyltransferase complex/magnesium transporter family protein )

HSP 1 Score: 459.5 bits (1181), Expect = 2.3e-129
Identity = 231/349 (66.19%), Postives = 282/349 (80.80%), Query Frame = 0

Query: 1   MATFFKFPHTPLILALLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSR 60
           MA   K      ++A L + FA  S S SDSDSD ++EL+ L+S S SGVIHL+D  +S+
Sbjct: 1   MALKSKLVSLLFLIATLSSTFAA-SFSDSDSDSDLLNELVSLRSTSESGVIHLDDHGISK 60

Query: 61  FLTSPKSPRPYSLLIFFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLF 120
           FLTS  +PRPYSLL+FFDA QLH K+EL L+ELRREFG+VS+SF+ANN   +    +KLF
Sbjct: 61  FLTSASTPRPYSLLVFFDATQLHSKNELRLQELRREFGIVSASFLANN---NGSEGTKLF 120

Query: 121 FCEIEFKESQPSFSLFGVNALPHVRLI--GTSQTPKHSDQMDQGDFSRLAESMAEFVESK 180
           FCEIEF +SQ SF LFGVNALPH+RL+    S     S QMDQ D+SRLAESMAEFVE +
Sbjct: 121 FCEIEFSKSQSSFQLFGVNALPHIRLVSPSISNLRDESGQMDQSDYSRLAESMAEFVEQR 180

Query: 181 TSLVIGPIHRPPLFSKNQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFF 240
           T L +GPI RPPL SK Q+ I++  ++I+ PF +++V+ GET+L+D ++WL GAIFIYFF
Sbjct: 181 TKLKVGPIQRPPLLSKPQIGIIVALIVIATPFIIKRVLKGETILHDTRLWLSGAIFIYFF 240

Query: 241 SVAGTMHNIIRKMPMFLADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITH 300
           SVAGTMHNIIRKMPMFL DRNDP+KL+FFYQGSGMQLGAEGFAVGFLYT+VGLLLAF+T+
Sbjct: 241 SVAGTMHNIIRKMPMFLQDRNDPNKLVFFYQGSGMQLGAEGFAVGFLYTVVGLLLAFVTN 300

Query: 301 VLVKVRNANTQRVVMMVALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSW 348
           VLV+V+N   QR++M++ALF+SFWAVKKVVYLDNWKTGYGIH YWPSSW
Sbjct: 301 VLVRVKNITAQRLIMLLALFISFWAVKKVVYLDNWKTGYGIHPYWPSSW 345

BLAST of MC01g1345 vs. TAIR 10
Match: AT1G11560.1 (Oligosaccharyltransferase complex/magnesium transporter family protein )

HSP 1 Score: 380.2 bits (975), Expect = 1.8e-105
Identity = 191/333 (57.36%), Postives = 254/333 (76.28%), Query Frame = 0

Query: 16  LLLTLFAQNSLSSSDSDSDQVSELLDLQSRSPSGVIHLNDQSVSRFLTSPKSPRPYSLLI 75
           L++ LF   +L++  SDSD  +EL+ L+S + SGVI  N+  VS+F+TS  +PRPYSL+I
Sbjct: 14  LIVFLF---TLANPKSDSDLKNELVSLRSTAESGVISFNNDDVSKFITSVSTPRPYSLII 73

Query: 76  FFDALQLHDKSELHLKELRREFGLVSSSFIANNPDPSSPSRSKLFFCEIEFKESQPSFSL 135
           FFDA+ LH  S+L L E RREF LVS++FI NN + S  + +KLFFCEIE   S+ SF  
Sbjct: 74  FFDAVHLHGNSQLRLPEFRREFRLVSATFITNNNNES--NGTKLFFCEIESTHSEASFRR 133

Query: 136 FGVNALPHVRLIG-TSQTPKHSDQMDQGDFSRLAESMAEFVESKTSLVIGPIHRPPLFSK 195
           F V +LPH+ L+  T++    SDQMD GDF+ LAESMAEFVE +T L +  I RPPL SK
Sbjct: 134 FAVESLPHISLVSPTTENLTESDQMDGGDFTGLAESMAEFVERQTKLTVCSIQRPPLISK 193

Query: 196 NQLAILIIAMLISAPFALRKVIAGETLLNDRKVWLVGAIFIYFFSVAGTMHNIIRKMPMF 255
            Q+ I++  ++IS P  ++K++ GETLL+D ++WLVGA+F+YFFSV+GTMHNIIR+MPM+
Sbjct: 194 TQIGIIVAIIIISTPILIKKILKGETLLHDHRIWLVGAVFVYFFSVSGTMHNIIREMPMY 253

Query: 256 LADRNDPSKLIFFYQGSGMQLGAEGFAVGFLYTIVGLLLAFITHVLVKVRNANTQRVVMM 315
           + D  D SK +FF + S MQLGAEGF VGFLYT+VGLLLAF+T+V+V+V+  + QR+ M+
Sbjct: 254 IKDYEDSSKFVFFIEESEMQLGAEGFFVGFLYTVVGLLLAFVTNVVVRVKKLDEQRMAML 313

Query: 316 VALFVSFWAVKKVVYLDNWKTGYGIHGYWPSSW 348
           +AL +SFWAV+KVVYLDNWKTGY I+ YWPSSW
Sbjct: 314 LALSISFWAVRKVVYLDNWKTGYEIYPYWPSSW 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYB53.3e-12866.19Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
Q94I552.5e-11259.76Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
F4I8X82.5e-10457.36Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
Q134542.5e-1122.48Tumor suppressor candidate 3 OS=Homo sapiens OX=9606 GN=TUSC3 PE=1 SV=1[more]
Q8BTV13.3e-1122.48Tumor suppressor candidate 3 OS=Mus musculus OX=10090 GN=Tusc3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038881671.12.60e-22090.52probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
TYK13063.16.86e-21890.38putative dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
XP_008439963.21.43e-21790.38PREDICTED: probable dolichyl-diphosphooligosaccharide--protein glycosyltransfera... [more]
XP_022978593.14.79e-21688.79probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
XP_004134751.11.32e-21589.50probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
Match NameE-valueIdentityDescription
A0A5D3CMB23.32e-21890.38Putative dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
A0A1S3B0R66.95e-21890.38probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
A0A6J1ITN12.32e-21688.79probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
A0A0A0KJY06.40e-21689.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G514900 PE=3 SV=1[more]
A0A6J1GE541.34e-21588.51probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ... [more]
Match NameE-valueIdentityDescription
AT1G61790.12.3e-12966.19Oligosaccharyltransferase complex/magnesium transporter family protein [more]
AT1G11560.11.8e-10557.36Oligosaccharyltransferase complex/magnesium transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 30..195
e-value: 5.7E-17
score: 63.9
NoneNo IPR availablePANTHERPTHR12692:SF5DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 3B-RELATEDcoord: 11..347
IPR021149Oligosaccharyl transferase complex, subunit OST3/OST6PFAMPF04756OST3_OST6coord: 38..337
e-value: 3.3E-77
score: 259.7
IPR021149Oligosaccharyl transferase complex, subunit OST3/OST6PANTHERPTHR12692DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATEDcoord: 11..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g1345.1MC01g1345.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018279 protein N-linked glycosylation via asparagine
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0008250 oligosaccharyltransferase complex
molecular_function GO:0016740 transferase activity