MC01g1330 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1330 vs. ExPASy Swiss-Prot
Match: Q8W4K1 (tRNA wybutosine-synthesizing protein 2/3/4 OS=Arabidopsis thaliana OX=3702 GN=At4g04670 PE=2 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 611/1083 (56.42%), Postives = 755/1083 (69.71%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            M+FEKRKAAT+AS+ S+ TDKSPKG LD PIIPL+ T+N HPSYFTTSSCSGRISILSQP
Sbjct: 1    MDFEKRKAATLASIRSSVTDKSPKGFLDEPIIPLLETINHHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTN----GELSEIVFRFEP 120
               ++      KKKA GGSWL +TH+ AD D V+ LLF S S       + SE+VFRFEP
Sbjct: 61   KPKSND---STKKKARGGSWLYITHDPADSDLVISLLFPSKSNQIDPIDQPSELVFRFEP 120

Query: 121  LIIAVECKDLGSAQALVSSAISCGFRESGITSA--SKRVIIAIRCSIRMEVPLGTTEKIM 180
            LIIAVECKDLGSAQ LV+ AIS GFRESGITS    KRVIIAIRCSIRMEVP+G TEK+M
Sbjct: 121  LIIAVECKDLGSAQFLVALAISAGFRESGITSCGDGKRVIIAIRCSIRMEVPIGDTEKLM 180

Query: 181  VSPEYVRYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDS 240
            VSPEYV++LVD+ANEKM+AN+KRTDGF      SV++ SN  +            D ND 
Sbjct: 181  VSPEYVKFLVDIANEKMDANRKRTDGF------SVALASNGFKN----------PDEND- 240

Query: 241  VDSKGHVCADEEDDGAALEGGDRNATGSSE----ACSHSLSVEQMDIVGEPMAKLFIWGH 300
                     DE+D+   L     ++  +           + +E++ IVGEP+ KL +WGH
Sbjct: 241  --------VDEDDNYENLAANHDSSINNGNLYPGVQKELIPLEKLSIVGEPVEKLHLWGH 300

Query: 301  SATTI----HNKVIVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSL 360
            SA TI      +VIVFGGFGG GRHARRN+ L L+ S  +L+ I V +SPS RLGHT+S+
Sbjct: 301  SACTIDESDRKEVIVFGGFGGFGRHARRNESLLLNPSCGTLKLIAVNESPSARLGHTASM 360

Query: 361  VGDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFG 420
            VGDF++VIGGR DP  ILNDVW  + +  +W+   C GS F PRHRHAAA+VG+K+Y+FG
Sbjct: 361  VGDFMFVIGGRADPLNILNDVWRLDISTGEWSSQRCVGSEFPPRHRHAAASVGTKVYIFG 420

Query: 421  GLENDRISSSLIVLDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGD 480
            GL ND+I SS+ +LDT   QWKE++  G+ PC RHSH+MV+ GS  +MFGGY+GE  L D
Sbjct: 421  GLYNDKIVSSMHILDTKDLQWKEVEQQGQWPCARHSHAMVAYGSQSFMFGGYNGENVLND 480

Query: 481  LYTFDADACFWKKENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHS 540
            LY+FD  +C WK E I+G+ P+ARFSH+MFVYK+ IGIIGGCPV+Q CQ+L+LLDL    
Sbjct: 481  LYSFDVQSCSWKLEVISGKWPHARFSHSMFVYKHTIGIIGGCPVSQNCQELTLLDLKHRL 540

Query: 541  WTHVSLKCIGKGLFVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVIL 600
            W  V L+ + K LFVRSTAS++GDD+++IGGGA+CYAFGT FSEP+KI L          
Sbjct: 541  WRSVRLEFMNKELFVRSTASILGDDLIVIGGGAACYAFGTKFSEPVKINL---------- 600

Query: 601  GHPETIEKVANHLIEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALY 660
               +++    NHL  + E  + +       NA    E S       +P            
Sbjct: 601  --VQSVTMSENHLPPQPEDVSLESN---KNNADLKTETS-----LSQP------------ 660

Query: 661  WILQIEKKYAKLGKDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGD 720
            W++Q+E+KYAK GKDILK+FGWLDL RKV S    G  ICFPV   F ++F EKQ     
Sbjct: 661  WVIQLERKYAKFGKDILKSFGWLDLERKVYSNEK-GLCICFPVTENFSELFHEKQ----- 720

Query: 721  QIYQKNDFHVSELVS-CEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKM 780
                  DF  SE  +  +G      S   ALN+LK+  A KL++   E K   KSP ++M
Sbjct: 721  --LLGKDFERSEENNLTKGLSLKDISCSAALNLLKEHGAKKLINVAFEAKKVAKSPLQRM 780

Query: 781  SEAVYSLIKHNGLSEELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFK 840
             E + S++K  GL EELL+ELP                     +WERLGDIVV+P TSFK
Sbjct: 781  REDITSILKQKGLPEELLDELPQ--------------------KWERLGDIVVVPATSFK 840

Query: 841  DPIWDTVGEELWPMIAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYS 900
            DP W ++ +E+W  ++KSL  +RLARQGRV   GTRDS LEILVGDNGWV+HRENGI YS
Sbjct: 841  DPTWSSINDEVWCAVSKSLSANRLARQGRVEPNGTRDSTLEILVGDNGWVNHRENGILYS 900

Query: 901  FDATKCMFSWGNLSEKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPH 960
            FDATKCMFSWGNLSEKLRM ++ C+ E +VDLFAGIGYFVLPFLV AKAKLVYACEWNPH
Sbjct: 901  FDATKCMFSWGNLSEKLRMGNMACENEVVVDLFAGIGYFVLPFLVRAKAKLVYACEWNPH 960

Query: 961  AIEALKRNLQANCVSNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEG 1020
            AIEAL+RN++AN VS RCIILEGDNR TAPKGVADRV LGL+P+SEGSWVTA++ LR EG
Sbjct: 961  AIEALRRNVEANSVSERCIILEGDNRITAPKGVADRVNLGLIPSSEGSWVTAIQALRPEG 995

Query: 1021 GMLHVHDNVKDSEEELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADV 1069
            G+LHVH NVKDS+E  W  H+ +++++IAR+EG  W+V++EH+E+VKWYAP IRHLVADV
Sbjct: 1021 GILHVHGNVKDSDESSWGEHVTKTLSDIARAEGRSWEVTVEHIEKVKWYAPRIRHLVADV 995

BLAST of MC01g1330 vs. ExPASy Swiss-Prot
Match: Q8H4D4 (tRNA wybutosine-synthesizing protein 2/3/4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0515000 PE=2 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 4.8e-303
Identity = 539/1087 (49.59%), Postives = 725/1087 (66.70%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQ- 60
            MEF++RKAA +A+L S   DKSPKG +D PI PL++ LN+HP  FTTSSCSGR+S+L+Q 
Sbjct: 1    MEFDRRKAAALAALASPAPDKSPKGGVDAPIAPLLDALNSHPDLFTTSSCSGRVSVLAQP 60

Query: 61   -PITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFR-SPSTNGELSEIVFRFEPL 120
             P   A     K KKKA GG W+ ++H+ ADP+++V++LF       G   E+VFRFEP+
Sbjct: 61   PPPQQADPGGAKTKKKARGGGWVYISHDPADPEALVEVLFGVKEGGGGGDDELVFRFEPM 120

Query: 121  IIAVECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSP 180
            I+AVEC+D  +A ALV++A+  GFRESGITS  KRV++A+RCSIRMEVPLG T++++VSP
Sbjct: 121  IVAVECRDAAAAAALVAAAVGAGFRESGITSLQKRVMVALRCSIRMEVPLGQTKELVVSP 180

Query: 181  EYVRYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDS 240
            +Y+RYLV +AN KMEANKKR  GF   +Q+ +S+ ++  ++   +L     G  +   ++
Sbjct: 181  DYIRYLVRIANSKMEANKKRMGGFLDLLQAKISLEASYLESQDPVLQN---GAKHGFGNA 240

Query: 241  KGHVC-------ADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGH 300
            K HV        A     G  L   +   T S    +H LS   ++I GEP+ KLF+WG 
Sbjct: 241  KRHVLISLSFYPAFISPHGVILTQEEALPTLSGNT-THCLSTAALEITGEPIEKLFLWGQ 300

Query: 301  SATTI----HNKVIVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSL 360
            SA  +     + ++ FGGFGG GRHARRN  L ++  S  L  + V  SPSPR+GHT ++
Sbjct: 301  SACALTVGREHHILTFGGFGGPGRHARRNYSLLVNPGSGLLTELKVTGSPSPRMGHTITV 360

Query: 361  VGDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFG 420
            VG+ IYV+GGR+ P+ ILND+WV   +  +W+ ++C+G  F PRHRHAAAAV  K+YVFG
Sbjct: 361  VGNDIYVVGGRSGPSEILNDIWVLERSNNRWSKVDCSGDFFRPRHRHAAAAVDRKVYVFG 420

Query: 421  GLENDRISSSLIVLDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGD 480
            GL +D + S + ++DT S QW  I    + PC RHSHS+VS GS +++FGG+DG++ L D
Sbjct: 421  GLSDDGLCSCMNIMDTASIQWNVISPDDKWPCARHSHSLVSYGSKLFLFGGHDGQRALND 480

Query: 481  LYTFDADACFWKKENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHS 540
             Y+FD     W KEN  G+ P+ RFSH MF+YK+Y+GI+GGCP+ ++ Q+++LL+L    
Sbjct: 481  FYSFDTTTLKWNKENTNGKAPSPRFSHCMFIYKDYLGILGGCPIRESSQEIALLNLKHKI 540

Query: 541  WTHVSLKCIGKGLFVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVIL 600
            W +VS+  + + L VRS++ ++ DD+V++GGGASCYAFGT FS+P+KI LH L S   + 
Sbjct: 541  WFYVSIPSLSQCLCVRSSSVIIDDDLVIVGGGASCYAFGTRFSQPIKIDLHLLESIFKLA 600

Query: 601  GHPETIEKVANHLIEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALY 660
             + E    V +  +  ++        LL G+ ++ N +    +  +   L      G+  
Sbjct: 601  YNKEKEMSVQHGSVSNVD--------LLEGHEENCNPSDNVKVVIDTATL------GSSP 660

Query: 661  WILQIEKKYAKLGKDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGD 720
             +LQ+EKKYAKL KDILK FGWLDL RKV   S    H+ FPV+  F  +  +K      
Sbjct: 661  LVLQLEKKYAKLAKDILKKFGWLDLTRKVRV-SQDNIHVLFPVSKTFHALITDKHL---- 720

Query: 721  QIYQKNDFHVSELVSCEGFRPNK-----CSYLEALNMLKKCDATKLVDEVVEIKTATKSP 780
            ++   +     EL+    F  NK      S  +AL +L  C  + L DEV   + A+K+P
Sbjct: 721  KVQPDDSCVFEELLP---FSENKLFGASISLQKALEILLLCRGSILKDEVAISRKASKTP 780

Query: 781  YKKMSEAVYSLIKHNGLSEELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPV 840
               M E V  L+   GL  +LLE+LPT                    RWE LGDI+VLP 
Sbjct: 781  QTIMRELVSVLLDKKGLPSQLLEQLPT--------------------RWETLGDIIVLPK 840

Query: 841  TSFKDPIWDTVGEELWPMIAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENG 900
            T FKDP+W++V ++LWP++AKSL   RLARQG++   GTRDS LE+LVG++GW+ H ENG
Sbjct: 841  TCFKDPLWESVRDDLWPLVAKSLGAQRLARQGKITPNGTRDSTLELLVGNDGWLTHHENG 900

Query: 901  IAYSFDATKCMFSWGNLSEKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACE 960
            I YS DATKCMFS GN SEKLRM  LDC++E +VDLFAGIGYFVLPFLV A AKLVYACE
Sbjct: 901  ICYSLDATKCMFSSGNRSEKLRMGKLDCRDEVVVDLFAGIGYFVLPFLVKANAKLVYACE 960

Query: 961  WNPHAIEALKRNLQANCVSNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTL 1020
            WNPHA+EAL+RN+  N V++RCIILEGDNR TAPKG+ADRVCLGLLP+SE SW TAVR L
Sbjct: 961  WNPHALEALQRNVMDNHVADRCIILEGDNRLTAPKGIADRVCLGLLPSSECSWDTAVRAL 1020

Query: 1021 RSEGGMLHVHDNVKDSEEELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHL 1069
            R+EGGMLH+H NV DS+E LW  ++++SI  IA++ G  W+V++EHVERVKWY PHIRHL
Sbjct: 1021 RAEGGMLHIHGNVNDSDESLWLDNVVKSITNIAKTHGLSWNVTVEHVERVKWYGPHIRHL 1041

BLAST of MC01g1330 vs. ExPASy Swiss-Prot
Match: Q4R3U8 (tRNA wybutosine-synthesizing protein 2 homolog OS=Macaca fascicularis OX=9541 GN=TRMT12 PE=2 SV=2)

HSP 1 Score: 253.8 bits (647), Expect = 8.5e-66
Identity = 145/378 (38.36%), Postives = 203/378 (53.70%), Query Frame = 0

Query: 742  CDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGL--SEELLEELPTRLDMILLRYHL 801
            C  T+L D V   +    SP +K+   V   ++  G+  S EL  +LP            
Sbjct: 84   CMLTRLPDPVPSKRAQGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPR----------- 143

Query: 802  LKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPMIAKSLCTSRLARQGRVASTG 861
                      W+R G++++L    F+   W  +G ELW  +A +L   RLA++GRV+  G
Sbjct: 144  ---------SWQRHGNLLLLSEDCFQANQWKNLGPELWETVASALGVQRLAKRGRVSPDG 203

Query: 862  TRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSEKLRMAHLDCKEETIVDLFA 921
            TR   + +L+GD+GWV+H +NGI Y FD T+CMFS+GN++EKLR+A L C  E +VDL+A
Sbjct: 204  TRTPAVTLLLGDHGWVEHVDNGILYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYA 263

Query: 922  GIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVSNRCIILEGDNRETAPKGVA 981
            GIGYF LPFLV A A  V+ACEWNPHA+ AL+ NL+ N V++RC I  GDNR+     +A
Sbjct: 264  GIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIA 323

Query: 982  DRVCLGLLPTSEGSWVTAVRTLRSE-GGMLHVHDNVKD-------------SEEELW--- 1041
            DRV LGL+P+SE  W  A + LR + GG+LH+H NV+              +E+E W   
Sbjct: 324  DRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQPLEVSKTEKEHWLYP 383

Query: 1042 ------------------------TRHLLQSIAEIARS---------EGHHWDVSIEHVE 1068
                                    T+   Q  AE A +          G  W   I H++
Sbjct: 384  QQITTNQWKNGATRDTRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQ 441

BLAST of MC01g1330 vs. ExPASy Swiss-Prot
Match: Q4V8B8 (tRNA wybutosine-synthesizing protein 2 homolog OS=Rattus norvegicus OX=10116 GN=Trmt12 PE=2 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 1.9e-65
Identity = 145/378 (38.36%), Postives = 203/378 (53.70%), Query Frame = 0

Query: 742  CDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGL--SEELLEELPTRLDMILLRYHL 801
            C  T+L+D +   K    SP +++   V   ++  G+  S EL  +LP            
Sbjct: 74   CKLTQLLDPLPSKKARVCSPAQRLCLEVRRWVEDRGVTWSTELEADLPR----------- 133

Query: 802  LKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPMIAKSLCTSRLARQGRVASTG 861
                      W+R GD+++L    F+   W  +  ELW  +A +L   RLA++GRV   G
Sbjct: 134  ---------SWQRHGDLMLLSEDCFQATQWKRLEPELWETVASALGVQRLAKRGRVLPDG 193

Query: 862  TRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSEKLRMAHLDCKEETIVDLFA 921
            TR   + +L+GD+GWV+H +NGI Y FD T+CMFS+GN++EKLR+A L C  E +VDL+A
Sbjct: 194  TRTPTVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYA 253

Query: 922  GIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVSNRCIILEGDNRETAPKGVA 981
            GIGYF LPFLV A A  V+ACEWNPHA+ AL++NL+ N V++RC I  GDNR+      A
Sbjct: 254  GIGYFTLPFLVHAGAAFVHACEWNPHAVVALRKNLEINGVADRCQIHFGDNRKLKLSNTA 313

Query: 982  DRVCLGLLPTSEGSWVTAVRTLRSE-GGMLHVHDNVK-------------DSEEELW--- 1041
            DRV LGL+P+SE  W  A + LR + GG+LH+H NV+             + E++ W   
Sbjct: 314  DRVNLGLIPSSEEGWPIACQVLRKDVGGILHIHQNVESFSGKNPQPPGSSNMEKKHWPHP 373

Query: 1042 -----------------------TRHLLQSIAEIARSE---------GHHWDVSIEHVER 1069
                                   ++   Q  AE A S+         G  W   I HV  
Sbjct: 374  QKITTDKQGNRTTGSCMGEMSSASKPEWQRWAESAESQIASLLHQVHGKPWRTRILHVHP 431

BLAST of MC01g1330 vs. ExPASy Swiss-Prot
Match: Q53H54 (tRNA wybutosine-synthesizing protein 2 homolog OS=Homo sapiens OX=9606 GN=TRMT12 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 3.2e-65
Identity = 145/378 (38.36%), Postives = 202/378 (53.44%), Query Frame = 0

Query: 742  CDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGL--SEELLEELPTRLDMILLRYHL 801
            C  T+L D V   +    SP +K+   V   ++  G+  S EL  +LP            
Sbjct: 84   CMLTQLPDPVPSKRAQGCSPAQKLCLEVSRWVEGRGVKWSAELEADLPR----------- 143

Query: 802  LKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPMIAKSLCTSRLARQGRVASTG 861
                      W+R G++++L    F+   W  +G ELW  +A +L   RLA++GRV+  G
Sbjct: 144  ---------SWQRHGNLLLLSEDCFQAKQWKNLGPELWETVALALGVQRLAKRGRVSPDG 203

Query: 862  TRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSEKLRMAHLDCKEETIVDLFA 921
            TR   + +L+GD+GWV+H +NGI Y FD T+CMFS+GN++EKLR+A L C  E +VDL+A
Sbjct: 204  TRTPAVTLLLGDHGWVEHVDNGIRYKFDVTQCMFSFGNITEKLRVASLSCAGEVLVDLYA 263

Query: 922  GIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVSNRCIILEGDNRETAPKGVA 981
            GIGYF LPFLV A A  V+ACEWNPHA+ AL+ NL+ N V++RC I  GDNR+     +A
Sbjct: 264  GIGYFTLPFLVHAGAAFVHACEWNPHAVVALRNNLEINGVADRCQIHFGDNRKLKLSNIA 323

Query: 982  DRVCLGLLPTSEGSWVTAVRTLRSE-GGMLHVHDNVKD-------------SEEELW--- 1041
            DRV LGL+P+SE  W  A + LR + GG+LH+H NV+               E+E W   
Sbjct: 324  DRVILGLIPSSEEGWPIACQVLRQDAGGILHIHQNVESFPGKNLQALGVSKVEKEHWLYP 383

Query: 1042 ------------------------TRHLLQSIAEIARS---------EGHHWDVSIEHVE 1068
                                    T+   Q  AE A +          G  W   I H++
Sbjct: 384  QQITTNQWKNGATRDSRGKMLSPATKPEWQRWAESAETRIATLLQQVHGKPWKTQILHIQ 441

BLAST of MC01g1330 vs. NCBI nr
Match: XP_022142199.1 (tRNA wybutosine-synthesizing protein 2/3/4 [Momordica charantia])

HSP 1 Score: 2131 bits (5522), Expect = 0.0
Identity = 1053/1073 (98.14%), Postives = 1053/1073 (98.14%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA
Sbjct: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH
Sbjct: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240

Query: 241  VCADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKVI 300
            VCADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKVI
Sbjct: 241  VCADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKVI 300

Query: 301  VFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTDP 360
            VFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTDP
Sbjct: 301  VFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTDP 360

Query: 361  TCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVL 420
            TCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVL
Sbjct: 361  TCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVL 420

Query: 421  DTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKKE 480
            DTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKKE
Sbjct: 421  DTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKKE 480

Query: 481  NIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGLF 540
            NIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGLF
Sbjct: 481  NIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGLF 540

Query: 541  VRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHLI 600
            VRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHLI
Sbjct: 541  VRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHLI 600

Query: 601  EELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLGK 660
            EELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLGK
Sbjct: 601  EELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLGK 660

Query: 661  DILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSELV 720
            DILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSELV
Sbjct: 661  DILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSELV 720

Query: 721  SCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLSE 780
            SCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLSE
Sbjct: 721  SCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLSE 780

Query: 781  ELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPMI 840
            ELLEELPTR                    WERLGDIVVLPVTSFKDPIWDTVGEELWPMI
Sbjct: 781  ELLEELPTR--------------------WERLGDIVVLPVTSFKDPIWDTVGEELWPMI 840

Query: 841  AKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSE 900
            AKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSE
Sbjct: 841  AKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSE 900

Query: 901  KLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVS 960
            KLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVS
Sbjct: 901  KLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVS 960

Query: 961  NRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEEE 1020
            NRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEEE
Sbjct: 961  NRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEEE 1020

Query: 1021 LWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQN 1073
            LWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQN
Sbjct: 1021 LWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQN 1053

BLAST of MC01g1330 vs. NCBI nr
Match: XP_023517513.1 (tRNA wybutosine-synthesizing protein 2/3/4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1793 bits (4645), Expect = 0.0
Identity = 885/1075 (82.33%), Postives = 956/1075 (88.93%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAA MASLGSTETDKSPKGSLDTPIIPLINTLN HPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAAAMASLGSTETDKSPKGSLDTPIIPLINTLNNHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            + S SA A KP+KKALGGSWL V+HEFADP+SV+DLLFRSPSTNGELSE+VFRFEPLIIA
Sbjct: 61   VCSTSAAAPKPRKKALGGSWLFVSHEFADPNSVIDLLFRSPSTNGELSELVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            +ECKDLGSAQ LVS+AIS GFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  IECKDLGSAQDLVSTAISSGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKM ANKKRTDGF K +QS V+ V+NVSQTC GI  REAM + ND +DSK H
Sbjct: 181  RYLVDVANEKMVANKKRTDGFLKALQSRVAAVTNVSQTCQGISLREAMEEANDLLDSKEH 240

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CADEED GA LEGG+RNA +GSSEAC++SLSVEQM I GEPMAKLFIWGHSATT+HNKV
Sbjct: 241  SCADEEDCGA-LEGGNRNANSGSSEACNYSLSVEQMGISGEPMAKLFIWGHSATTVHNKV 300

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLLQLDM SC+LQTINVEDSPSPRLGHTSSLVGD +YVIGGRTD
Sbjct: 301  IAFGGFGGMGRHARRNDLLQLDMLSCTLQTINVEDSPSPRLGHTSSLVGDCLYVIGGRTD 360

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PT ILNDVWVF+ T EKWTLL+CTGS FSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV
Sbjct: 361  PTYILNDVWVFDITQEKWTLLKCTGSSFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            L+TD+HQWKEIQAGG+ PCGRHSHSMVS GSH+YMFGGYDGEK  GDLY+F+ + CFWKK
Sbjct: 421  LNTDTHQWKEIQAGGDQPCGRHSHSMVSYGSHLYMFGGYDGEKAFGDLYSFNTENCFWKK 480

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            ENIAGRTPNARFSHAMFVY+NYIGIIGGCPV+Q+ Q+ +LLDL    W HVSL C GK L
Sbjct: 481  ENIAGRTPNARFSHAMFVYENYIGIIGGCPVSQSYQEFALLDLQHRFWRHVSLNCTGKEL 540

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVR TA+VVGDD+VL+GGGASCYAFGT FSEP KIRL PL+SSEVILGHP   E +  H 
Sbjct: 541  FVRCTANVVGDDLVLVGGGASCYAFGTNFSEPAKIRLRPLISSEVILGHPRNTENLVTHP 600

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
            IEELE+++RDPECLLNGNAQ+FNEASGF I+ EK N HE  ++GALYW+L+IE+ YAKLG
Sbjct: 601  IEELEKESRDPECLLNGNAQTFNEASGFNIDSEKSNSHE--KEGALYWVLKIERNYAKLG 660

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK FGWLDL RKVSSR SG THI FPVN KFCD+FDEK+SWWGDQ+  +NDFH+SEL
Sbjct: 661  KDILKRFGWLDLARKVSSRGSG-THIFFPVNGKFCDMFDEKKSWWGDQLEPENDFHISEL 720

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG   NKCSYL+ALN+L+KC ATKLVDEVV+IKTA KSPYKKMSEAV SL+KHNG S
Sbjct: 721  ESWEGGLTNKCSYLKALNILRKCSATKLVDEVVDIKTAAKSPYKKMSEAVSSLLKHNGSS 780

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            E LLEELPTR                    WERLGDIVVLPVTSFKDP WDT+GEELWP+
Sbjct: 781  EGLLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGEELWPL 840

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL T RLARQGRVASTGTRDSNLEILVGDNGWVDHRENGI YSFDATKCMFSWGNLS
Sbjct: 841  VAKSLGTCRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGITYSFDATKCMFSWGNLS 900

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHLDCK+ET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV
Sbjct: 901  EKLRMAHLDCKDETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRCI+LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LR EGG LHVH NVKDSEE
Sbjct: 961  SNRCIVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRIEGGTLHVHGNVKDSEE 1020

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            ELWT+HL  SI EIA+SEGH WDVS+EH+ERVKWYAPHIRHLV DV+C+RIQQNS
Sbjct: 1021 ELWTQHLRHSITEIAKSEGHCWDVSVEHIERVKWYAPHIRHLVVDVRCKRIQQNS 1051

BLAST of MC01g1330 vs. NCBI nr
Match: KAG6594662.1 (tRNA wybutosine-synthesizing protein 2/3/4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1792 bits (4641), Expect = 0.0
Identity = 885/1075 (82.33%), Postives = 956/1075 (88.93%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN HPSYFTTSSCSGRISILSQP
Sbjct: 113  MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNNHPSYFTTSSCSGRISILSQP 172

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            + S SA A KP+KKALGGSWL V+HEFADP+SV+DLLFRSPSTNGELSE+VFRFEPLIIA
Sbjct: 173  VCSTSAAAPKPRKKALGGSWLFVSHEFADPNSVIDLLFRSPSTNGELSELVFRFEPLIIA 232

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            +ECKDLGSAQ LVS+AIS GFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 233  IECKDLGSAQDLVSTAISSGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 292

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKM ANKKRTDGF K +QS VS V+NVSQTC GI  REAM + ND +DSK H
Sbjct: 293  RYLVDVANEKMVANKKRTDGFLKALQSRVSAVTNVSQTCQGISLREAMEEANDLLDSKEH 352

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CADEED GA LEGG+RN  +GSSEAC++SLSVEQM I GEPMAKLFIWGHSATT+HNKV
Sbjct: 353  SCADEEDCGA-LEGGNRNPNSGSSEACNYSLSVEQMGISGEPMAKLFIWGHSATTVHNKV 412

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRH RRNDLLQLDM SC+LQTINVEDSPSPRLGHTSSLVGD +YVIGGRTD
Sbjct: 413  IAFGGFGGMGRHGRRNDLLQLDMLSCTLQTINVEDSPSPRLGHTSSLVGDCLYVIGGRTD 472

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PT ILNDVWVF+ T EKWTLL+CTGS FSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV
Sbjct: 473  PTYILNDVWVFDITQEKWTLLKCTGSSFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 532

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            L+TDSHQWKEIQAGG+ PCGRHSHSMVS GSH+YMFGGYDGEK  GDLY+F+ + C+WKK
Sbjct: 533  LNTDSHQWKEIQAGGDQPCGRHSHSMVSYGSHLYMFGGYDGEKAFGDLYSFNTENCYWKK 592

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            EN+AGRTPNARFSHAMFVY+NYIGIIGGCPV+Q+ Q+ +LLDL    W HVSL CIGK L
Sbjct: 593  ENMAGRTPNARFSHAMFVYENYIGIIGGCPVSQSYQEFALLDLQHRFWRHVSLNCIGKEL 652

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVR TA+VVGDD+VL+GGGASCYAFGT FSEP+KIRL PL+SSEVILGHP   E +    
Sbjct: 653  FVRCTANVVGDDLVLVGGGASCYAFGTNFSEPVKIRLRPLISSEVILGHPRNTENLVTRP 712

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
            IEEL++++RDPECLLN NAQ+FNEASGF I+ EK N HE  ++GALYW+L+IE+ YAKLG
Sbjct: 713  IEELQKESRDPECLLNSNAQTFNEASGFNIDSEKSNSHE--KEGALYWVLKIERNYAKLG 772

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK +GWLDL RKVSSR SG THI FPVN KFCDIFDEK+SWWGDQ+  +NDFHVSEL
Sbjct: 773  KDILKRYGWLDLARKVSSRGSG-THIFFPVNGKFCDIFDEKKSWWGDQLEPENDFHVSEL 832

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG   NKCSYL+ALN+L+KC ATKLVDEVV+IKTA KSPYKKMSEAV SL+KHNGLS
Sbjct: 833  ESWEGGLTNKCSYLKALNILRKCGATKLVDEVVDIKTAAKSPYKKMSEAVSSLLKHNGLS 892

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            E  LEELPTR                    WERLGDIVVLPVTSFKDP WDT+GEELWPM
Sbjct: 893  EGFLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGEELWPM 952

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL TSRLARQGRVASTGTRDSNLEIL+GDNGWVDHRENGI YSFDATKCMFSWGNLS
Sbjct: 953  VAKSLGTSRLARQGRVASTGTRDSNLEILMGDNGWVDHRENGITYSFDATKCMFSWGNLS 1012

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHLDCK+ET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV
Sbjct: 1013 EKLRMAHLDCKDETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 1072

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRCI+LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LR EGG LHVH NVKDSEE
Sbjct: 1073 SNRCIVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRIEGGTLHVHGNVKDSEE 1132

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            ELWT+HLL SI EIA+SEGH WDVS+EH ERVKWYAPHIRHLV DV+C+RIQQNS
Sbjct: 1133 ELWTQHLLHSITEIAKSEGHCWDVSVEHTERVKWYAPHIRHLVVDVRCKRIQQNS 1163

BLAST of MC01g1330 vs. NCBI nr
Match: XP_022926928.1 (tRNA wybutosine-synthesizing protein 2/3/4 [Cucurbita moschata])

HSP 1 Score: 1786 bits (4627), Expect = 0.0
Identity = 883/1075 (82.14%), Postives = 956/1075 (88.93%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN HPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNNHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            + S SA A KP+KKALGGSWL V+HEFADP+SV+DLLFRSPSTNGELSE+VFRFEPLIIA
Sbjct: 61   VCSTSAAAPKPRKKALGGSWLFVSHEFADPNSVIDLLFRSPSTNGELSELVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            +ECKDLGSAQ LVS+AIS GFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  IECKDLGSAQDLVSTAISSGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKM ANKKRTDGF K +QS VS V+NVSQTC GI  REAM + ND +DSK H
Sbjct: 181  RYLVDVANEKMVANKKRTDGFLKALQSRVSAVTNVSQTCQGISLREAMEEANDLLDSKEH 240

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CADEED GA LEGG+RNA +GSSEAC++SLSVEQM I GEPMAKLFIWGHSATT+HNKV
Sbjct: 241  SCADEEDCGA-LEGGNRNANSGSSEACNYSLSVEQMGISGEPMAKLFIWGHSATTVHNKV 300

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLLQLDM SC+LQTI+VEDSPSPRLGHTSSLVGD +YVIGGRTD
Sbjct: 301  IAFGGFGGMGRHARRNDLLQLDMLSCTLQTISVEDSPSPRLGHTSSLVGDCLYVIGGRTD 360

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PT ILNDVWVF+ T  KWTLL+CTGS FSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV
Sbjct: 361  PTYILNDVWVFDITQAKWTLLKCTGSSFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            L+TDSHQWKEIQAGG+ PCGRHSHSMVS GSH+YMFGGYDGEK  GDLY+F+ + C+WKK
Sbjct: 421  LNTDSHQWKEIQAGGDQPCGRHSHSMVSYGSHLYMFGGYDGEKAFGDLYSFNTENCYWKK 480

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            EN+AGRTPNARFSHAMFVY+NYIGIIGGCPV+Q+ Q+ +LLDL    W HVSL C GK L
Sbjct: 481  ENMAGRTPNARFSHAMFVYENYIGIIGGCPVSQSYQEFALLDLQHRFWRHVSLNCTGKEL 540

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVR TA+VVGDD+VL+GGGASCYAFGT FSEP+KIRL PL+SSEVILGHP   E +    
Sbjct: 541  FVRCTANVVGDDLVLVGGGASCYAFGTNFSEPVKIRLRPLISSEVILGHPRNTENLVTRP 600

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
            IEELE+++RDPECLLNGNAQ+FNEA GF I+ EK N HE  ++GALYW+L+IE+ YAKLG
Sbjct: 601  IEELEKESRDPECLLNGNAQTFNEAFGFNIDSEKSNSHE--KEGALYWVLKIERNYAKLG 660

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK +GWLD+ RKVSSR SG THI FPVN KFCDIFDEK+SWWGDQ+  +NDFHVSEL
Sbjct: 661  KDILKRYGWLDMVRKVSSRGSG-THIFFPVNGKFCDIFDEKKSWWGDQLEPENDFHVSEL 720

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG    KCSYL+ALN+L+KC ATKLVDEVV+IKTA KSPYKKMSEA+ SL+KHNGLS
Sbjct: 721  ESWEGGLTKKCSYLKALNILRKCGATKLVDEVVDIKTAAKSPYKKMSEALSSLLKHNGLS 780

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            E LLEELPTR                    WERLGDIVVLPVTSFKDP WDT+GEELWPM
Sbjct: 781  EGLLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGEELWPM 840

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL TSRLARQGRVASTGTRDSNLEIL+GDNGWVDHRENGI YSFDATKCMFSWGNLS
Sbjct: 841  VAKSLGTSRLARQGRVASTGTRDSNLEILMGDNGWVDHRENGITYSFDATKCMFSWGNLS 900

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHLDCK+ET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV
Sbjct: 901  EKLRMAHLDCKDETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRCI+LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LR EGG LHVH NVKDSEE
Sbjct: 961  SNRCIVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRIEGGTLHVHGNVKDSEE 1020

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            ELWT+HLL SI EIA+SEGH WDVS+EH ERVKWYAPHIRHLV DV+C+RIQQNS
Sbjct: 1021 ELWTQHLLHSITEIAKSEGHCWDVSVEHTERVKWYAPHIRHLVVDVRCKRIQQNS 1051

BLAST of MC01g1330 vs. NCBI nr
Match: XP_023002927.1 (tRNA wybutosine-synthesizing protein 2/3/4 [Cucurbita maxima])

HSP 1 Score: 1784 bits (4620), Expect = 0.0
Identity = 884/1075 (82.23%), Postives = 954/1075 (88.74%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN HPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNNHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            + S SA A KP+KKALGGSWL V+HEFADP+SV+DLLFRSPSTNGELSE+VFRFEPLIIA
Sbjct: 61   VCSTSAAASKPRKKALGGSWLFVSHEFADPNSVIDLLFRSPSTNGELSELVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            +ECKDLGSAQ LVS+AIS GFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  IECKDLGSAQDLVSTAISSGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKM ANKKRTDGF K +QS VS V+ VSQTC GI  REAM + ND +DSK H
Sbjct: 181  RYLVDVANEKMVANKKRTDGFLKALQSRVSAVTYVSQTCQGISLREAMEEANDLLDSKEH 240

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CADEED GA LEGG+RNA +GSSEAC++SLSVEQM I GEPMAKLFIWGHSATT+H KV
Sbjct: 241  SCADEEDCGA-LEGGNRNANSGSSEACNYSLSVEQMGISGEPMAKLFIWGHSATTVHKKV 300

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLLQLDM SC+LQTINVEDSPSPRLGHTSSLVGD +YVIGGRTD
Sbjct: 301  ISFGGFGGMGRHARRNDLLQLDMLSCTLQTINVEDSPSPRLGHTSSLVGDCLYVIGGRTD 360

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PT ILNDVWVF+ T EKWTLL+CTGS FSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV
Sbjct: 361  PTYILNDVWVFDITQEKWTLLKCTGSSFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            L+T+SHQWKEIQAGG+ PCGRHSHSMVS GSH+YMFGGYDGEK  GDLY+F+ + CFWKK
Sbjct: 421  LNTNSHQWKEIQAGGDQPCGRHSHSMVSYGSHLYMFGGYDGEKAFGDLYSFNTENCFWKK 480

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            ENIAGRTPNARFSHAMFVY+NYIGIIGGCPV+Q+ Q+L+LLDL    W HVSL C GK L
Sbjct: 481  ENIAGRTPNARFSHAMFVYENYIGIIGGCPVSQSYQELALLDLQHRFWRHVSLNCTGKEL 540

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVR TA+VVGDD+VL+GGGASCYAFGT FSEP+KIRL PL+SSEVILGHP   E +    
Sbjct: 541  FVRCTANVVGDDLVLVGGGASCYAFGTNFSEPVKIRLRPLISSEVILGHPRNTENLVTRP 600

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
            IEELE+++RDPECLLNGNAQ+FNEASGF I+  K N HE  ++GALYW+L+IE+ YAKLG
Sbjct: 601  IEELEKESRDPECLLNGNAQTFNEASGFNIDSGKSNSHE--KEGALYWVLKIERNYAKLG 660

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK +GWLDL RKVSSR S  THICFPV  KFCDIFDEK+SWW DQ+  +NDFHVSEL
Sbjct: 661  KDILKRYGWLDLARKVSSRGSR-THICFPVIGKFCDIFDEKKSWWADQLEPENDFHVSEL 720

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG   NKCSYL+ALN+L+KC ATKLVDEVV+IKTA KSPYKKMSEAV SL+KHNGLS
Sbjct: 721  ESWEGGLTNKCSYLKALNILRKCGATKLVDEVVDIKTAAKSPYKKMSEAVSSLLKHNGLS 780

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            EELLEELPTR                    WERLGDIVVLPVTSFKDP WDT+G ELWPM
Sbjct: 781  EELLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGVELWPM 840

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL  SRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGI YSFDATKCMFSWGNLS
Sbjct: 841  VAKSLGISRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGITYSFDATKCMFSWGNLS 900

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHLDCK+ET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHA+EAL RNLQANCV
Sbjct: 901  EKLRMAHLDCKDETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHALEALTRNLQANCV 960

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRCI+LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LR EGG LHVH NVKDSEE
Sbjct: 961  SNRCIVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRLEGGTLHVHGNVKDSEE 1020

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            ELWT+HLL SI EIA+SEGH WDVS+EH+ERVKWYAPHIRHLV DV+C+RIQQNS
Sbjct: 1021 ELWTQHLLHSITEIAKSEGHCWDVSVEHIERVKWYAPHIRHLVVDVRCKRIQQNS 1051

BLAST of MC01g1330 vs. ExPASy TrEMBL
Match: A0A6J1CKW3 (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase OS=Momordica charantia OX=3673 GN=LOC111012371 PE=4 SV=1)

HSP 1 Score: 2131 bits (5522), Expect = 0.0
Identity = 1053/1073 (98.14%), Postives = 1053/1073 (98.14%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA
Sbjct: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH
Sbjct: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240

Query: 241  VCADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKVI 300
            VCADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKVI
Sbjct: 241  VCADEEDDGAALEGGDRNATGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKVI 300

Query: 301  VFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTDP 360
            VFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTDP
Sbjct: 301  VFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTDP 360

Query: 361  TCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVL 420
            TCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVL
Sbjct: 361  TCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVL 420

Query: 421  DTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKKE 480
            DTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKKE
Sbjct: 421  DTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKKE 480

Query: 481  NIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGLF 540
            NIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGLF
Sbjct: 481  NIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGLF 540

Query: 541  VRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHLI 600
            VRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHLI
Sbjct: 541  VRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHLI 600

Query: 601  EELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLGK 660
            EELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLGK
Sbjct: 601  EELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLGK 660

Query: 661  DILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSELV 720
            DILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSELV
Sbjct: 661  DILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSELV 720

Query: 721  SCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLSE 780
            SCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLSE
Sbjct: 721  SCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLSE 780

Query: 781  ELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPMI 840
            ELLEELPTR                    WERLGDIVVLPVTSFKDPIWDTVGEELWPMI
Sbjct: 781  ELLEELPTR--------------------WERLGDIVVLPVTSFKDPIWDTVGEELWPMI 840

Query: 841  AKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSE 900
            AKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSE
Sbjct: 841  AKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLSE 900

Query: 901  KLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVS 960
            KLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVS
Sbjct: 901  KLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCVS 960

Query: 961  NRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEEE 1020
            NRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEEE
Sbjct: 961  NRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEEE 1020

Query: 1021 LWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQN 1073
            LWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQN
Sbjct: 1021 LWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQN 1053

BLAST of MC01g1330 vs. ExPASy TrEMBL
Match: A0A6J1EJK3 (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111433898 PE=4 SV=1)

HSP 1 Score: 1786 bits (4627), Expect = 0.0
Identity = 883/1075 (82.14%), Postives = 956/1075 (88.93%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN HPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNNHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            + S SA A KP+KKALGGSWL V+HEFADP+SV+DLLFRSPSTNGELSE+VFRFEPLIIA
Sbjct: 61   VCSTSAAAPKPRKKALGGSWLFVSHEFADPNSVIDLLFRSPSTNGELSELVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            +ECKDLGSAQ LVS+AIS GFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  IECKDLGSAQDLVSTAISSGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKM ANKKRTDGF K +QS VS V+NVSQTC GI  REAM + ND +DSK H
Sbjct: 181  RYLVDVANEKMVANKKRTDGFLKALQSRVSAVTNVSQTCQGISLREAMEEANDLLDSKEH 240

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CADEED GA LEGG+RNA +GSSEAC++SLSVEQM I GEPMAKLFIWGHSATT+HNKV
Sbjct: 241  SCADEEDCGA-LEGGNRNANSGSSEACNYSLSVEQMGISGEPMAKLFIWGHSATTVHNKV 300

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLLQLDM SC+LQTI+VEDSPSPRLGHTSSLVGD +YVIGGRTD
Sbjct: 301  IAFGGFGGMGRHARRNDLLQLDMLSCTLQTISVEDSPSPRLGHTSSLVGDCLYVIGGRTD 360

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PT ILNDVWVF+ T  KWTLL+CTGS FSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV
Sbjct: 361  PTYILNDVWVFDITQAKWTLLKCTGSSFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            L+TDSHQWKEIQAGG+ PCGRHSHSMVS GSH+YMFGGYDGEK  GDLY+F+ + C+WKK
Sbjct: 421  LNTDSHQWKEIQAGGDQPCGRHSHSMVSYGSHLYMFGGYDGEKAFGDLYSFNTENCYWKK 480

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            EN+AGRTPNARFSHAMFVY+NYIGIIGGCPV+Q+ Q+ +LLDL    W HVSL C GK L
Sbjct: 481  ENMAGRTPNARFSHAMFVYENYIGIIGGCPVSQSYQEFALLDLQHRFWRHVSLNCTGKEL 540

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVR TA+VVGDD+VL+GGGASCYAFGT FSEP+KIRL PL+SSEVILGHP   E +    
Sbjct: 541  FVRCTANVVGDDLVLVGGGASCYAFGTNFSEPVKIRLRPLISSEVILGHPRNTENLVTRP 600

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
            IEELE+++RDPECLLNGNAQ+FNEA GF I+ EK N HE  ++GALYW+L+IE+ YAKLG
Sbjct: 601  IEELEKESRDPECLLNGNAQTFNEAFGFNIDSEKSNSHE--KEGALYWVLKIERNYAKLG 660

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK +GWLD+ RKVSSR SG THI FPVN KFCDIFDEK+SWWGDQ+  +NDFHVSEL
Sbjct: 661  KDILKRYGWLDMVRKVSSRGSG-THIFFPVNGKFCDIFDEKKSWWGDQLEPENDFHVSEL 720

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG    KCSYL+ALN+L+KC ATKLVDEVV+IKTA KSPYKKMSEA+ SL+KHNGLS
Sbjct: 721  ESWEGGLTKKCSYLKALNILRKCGATKLVDEVVDIKTAAKSPYKKMSEALSSLLKHNGLS 780

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            E LLEELPTR                    WERLGDIVVLPVTSFKDP WDT+GEELWPM
Sbjct: 781  EGLLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGEELWPM 840

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL TSRLARQGRVASTGTRDSNLEIL+GDNGWVDHRENGI YSFDATKCMFSWGNLS
Sbjct: 841  VAKSLGTSRLARQGRVASTGTRDSNLEILMGDNGWVDHRENGITYSFDATKCMFSWGNLS 900

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHLDCK+ET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV
Sbjct: 901  EKLRMAHLDCKDETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRCI+LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LR EGG LHVH NVKDSEE
Sbjct: 961  SNRCIVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRIEGGTLHVHGNVKDSEE 1020

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            ELWT+HLL SI EIA+SEGH WDVS+EH ERVKWYAPHIRHLV DV+C+RIQQNS
Sbjct: 1021 ELWTQHLLHSITEIAKSEGHCWDVSVEHTERVKWYAPHIRHLVVDVRCKRIQQNS 1051

BLAST of MC01g1330 vs. ExPASy TrEMBL
Match: A0A6J1KKZ3 (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111496687 PE=4 SV=1)

HSP 1 Score: 1784 bits (4620), Expect = 0.0
Identity = 884/1075 (82.23%), Postives = 954/1075 (88.74%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN HPSYFTTSSCSGRISILSQP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNNHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            + S SA A KP+KKALGGSWL V+HEFADP+SV+DLLFRSPSTNGELSE+VFRFEPLIIA
Sbjct: 61   VCSTSAAASKPRKKALGGSWLFVSHEFADPNSVIDLLFRSPSTNGELSELVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            +ECKDLGSAQ LVS+AIS GFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV
Sbjct: 121  IECKDLGSAQDLVSTAISSGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            RYLVDVANEKM ANKKRTDGF K +QS VS V+ VSQTC GI  REAM + ND +DSK H
Sbjct: 181  RYLVDVANEKMVANKKRTDGFLKALQSRVSAVTYVSQTCQGISLREAMEEANDLLDSKEH 240

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CADEED GA LEGG+RNA +GSSEAC++SLSVEQM I GEPMAKLFIWGHSATT+H KV
Sbjct: 241  SCADEEDCGA-LEGGNRNANSGSSEACNYSLSVEQMGISGEPMAKLFIWGHSATTVHKKV 300

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLLQLDM SC+LQTINVEDSPSPRLGHTSSLVGD +YVIGGRTD
Sbjct: 301  ISFGGFGGMGRHARRNDLLQLDMLSCTLQTINVEDSPSPRLGHTSSLVGDCLYVIGGRTD 360

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PT ILNDVWVF+ T EKWTLL+CTGS FSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV
Sbjct: 361  PTYILNDVWVFDITQEKWTLLKCTGSSFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            L+T+SHQWKEIQAGG+ PCGRHSHSMVS GSH+YMFGGYDGEK  GDLY+F+ + CFWKK
Sbjct: 421  LNTNSHQWKEIQAGGDQPCGRHSHSMVSYGSHLYMFGGYDGEKAFGDLYSFNTENCFWKK 480

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            ENIAGRTPNARFSHAMFVY+NYIGIIGGCPV+Q+ Q+L+LLDL    W HVSL C GK L
Sbjct: 481  ENIAGRTPNARFSHAMFVYENYIGIIGGCPVSQSYQELALLDLQHRFWRHVSLNCTGKEL 540

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVR TA+VVGDD+VL+GGGASCYAFGT FSEP+KIRL PL+SSEVILGHP   E +    
Sbjct: 541  FVRCTANVVGDDLVLVGGGASCYAFGTNFSEPVKIRLRPLISSEVILGHPRNTENLVTRP 600

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
            IEELE+++RDPECLLNGNAQ+FNEASGF I+  K N HE  ++GALYW+L+IE+ YAKLG
Sbjct: 601  IEELEKESRDPECLLNGNAQTFNEASGFNIDSGKSNSHE--KEGALYWVLKIERNYAKLG 660

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK +GWLDL RKVSSR S  THICFPV  KFCDIFDEK+SWW DQ+  +NDFHVSEL
Sbjct: 661  KDILKRYGWLDLARKVSSRGSR-THICFPVIGKFCDIFDEKKSWWADQLEPENDFHVSEL 720

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG   NKCSYL+ALN+L+KC ATKLVDEVV+IKTA KSPYKKMSEAV SL+KHNGLS
Sbjct: 721  ESWEGGLTNKCSYLKALNILRKCGATKLVDEVVDIKTAAKSPYKKMSEAVSSLLKHNGLS 780

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            EELLEELPTR                    WERLGDIVVLPVTSFKDP WDT+G ELWPM
Sbjct: 781  EELLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGVELWPM 840

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL  SRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGI YSFDATKCMFSWGNLS
Sbjct: 841  VAKSLGISRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGITYSFDATKCMFSWGNLS 900

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHLDCK+ET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHA+EAL RNLQANCV
Sbjct: 901  EKLRMAHLDCKDETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHALEALTRNLQANCV 960

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRCI+LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LR EGG LHVH NVKDSEE
Sbjct: 961  SNRCIVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRLEGGTLHVHGNVKDSEE 1020

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            ELWT+HLL SI EIA+SEGH WDVS+EH+ERVKWYAPHIRHLV DV+C+RIQQNS
Sbjct: 1021 ELWTQHLLHSITEIAKSEGHCWDVSVEHIERVKWYAPHIRHLVVDVRCKRIQQNS 1051

BLAST of MC01g1330 vs. ExPASy TrEMBL
Match: A0A0A0KHU3 (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G513770 PE=4 SV=1)

HSP 1 Score: 1763 bits (4566), Expect = 0.0
Identity = 877/1075 (81.58%), Postives = 955/1075 (88.84%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN+HPSYFTTSSCSGRISIL+QP
Sbjct: 69   MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNSHPSYFTTSSCSGRISILAQP 128

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            I++ SA A KPKKKALGGSWL V+HEFA+P+SV+DLLFRSPSTN ELSE+VFRFEPLIIA
Sbjct: 129  ISTTSAAAPKPKKKALGGSWLFVSHEFAEPNSVIDLLFRSPSTNRELSELVFRFEPLIIA 188

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            VECKDLGSAQALVS+AISCGFRESGITSASKRVIIAIRCSIRMEVPLGT+EKIMV+PEYV
Sbjct: 189  VECKDLGSAQALVSTAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTSEKIMVTPEYV 248

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            +YLV+VANEKM ANKKRTDGF K +QSS+S   + S+TCHGI SREA  +VNDS+DSKGH
Sbjct: 249  QYLVNVANEKMVANKKRTDGFLKGLQSSIS---DASRTCHGIPSREATENVNDSLDSKGH 308

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CAD  DDGAALEGG RNA +GSSEACS+SLSVEQ+DI GEP+ KLFIWGHSATTIH+KV
Sbjct: 309  DCADG-DDGAALEGGVRNANSGSSEACSYSLSVEQIDIAGEPIEKLFIWGHSATTIHDKV 368

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLL LDM S +LQTINVEDSPSPRLGHTSSLVGD +YV+GGRTD
Sbjct: 369  IAFGGFGGMGRHARRNDLLLLDMLSYTLQTINVEDSPSPRLGHTSSLVGDRLYVVGGRTD 428

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PTCILNDVW+FN T EKWTLLECTGSPFSPRHRHAAAA+GSKIYVFGGLENDRISSS I 
Sbjct: 429  PTCILNDVWLFNITQEKWTLLECTGSPFSPRHRHAAAALGSKIYVFGGLENDRISSSFIF 488

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            LD+DSHQWKEIQAGGE PCGRHSHSMVS GSHIYMFGGYDGEKTLGDLY+FD +AC+WKK
Sbjct: 489  LDSDSHQWKEIQAGGEQPCGRHSHSMVSYGSHIYMFGGYDGEKTLGDLYSFDTNACYWKK 548

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            ENIAG TPNARFSHAMFVYKNYIGIIGGCPVTQT Q+L+LLDL L  W HVSL C G+ L
Sbjct: 549  ENIAGTTPNARFSHAMFVYKNYIGIIGGCPVTQTYQELALLDLQLRCWRHVSLNCTGREL 608

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVRST SVVG+D++L+GGGASCYAFGT FSEPMKIRLHPL+SSEV+LGH    EK     
Sbjct: 609  FVRSTVSVVGNDLILVGGGASCYAFGTTFSEPMKIRLHPLISSEVVLGHSGNTEK----- 668

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
               LE+ NRDP+C+ NGNAQSFNEA GF I+ EK N HEQKQ GALYW+LQIE+KYAKL 
Sbjct: 669  ---LEKANRDPKCMPNGNAQSFNEAFGFNIDFEKSNSHEQKQ-GALYWVLQIERKYAKLV 728

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK FGWLD+GR VSSR SG THICFPVN KFCD FDEKQSWW DQ+ Q+NDF +S  
Sbjct: 729  KDILKKFGWLDMGRNVSSRGSG-THICFPVNVKFCDTFDEKQSWWADQLEQENDFRISGP 788

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG      S L+ALN+LKKC ATKLVDEVV+IKTA K+P+KKMSEA+ SL+KHNGLS
Sbjct: 789  ESWEGCLT---SNLKALNVLKKCGATKLVDEVVDIKTAAKTPFKKMSEAMSSLLKHNGLS 848

Query: 781  EELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFKDPIWDTVGEELWPM 840
            EELLEELPTR                    WERLGDIVVLPVTSFKDP WDT+GEELWP+
Sbjct: 849  EELLEELPTR--------------------WERLGDIVVLPVTSFKDPTWDTIGEELWPL 908

Query: 841  IAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFSWGNLS 900
            +AKSL T RLARQGRVASTGTRDSNLEIL+GDNGWV+HRENGI YSFDATKCMFSWGNLS
Sbjct: 909  VAKSLGTYRLARQGRVASTGTRDSNLEILLGDNGWVEHRENGITYSFDATKCMFSWGNLS 968

Query: 901  EKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 960
            EKLRMAHL+CKEET+VDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV
Sbjct: 969  EKLRMAHLNCKEETVVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNLQANCV 1028

Query: 961  SNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNVKDSEE 1020
            SNRC++LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LRSEGG LHVHDNVKDSEE
Sbjct: 1029 SNRCVVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRSEGGTLHVHDNVKDSEE 1088

Query: 1021 ELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            E WT+ L+ SI EIA+SEGH WD++IEH+ERVKWYAPHIRHLVADV+C+RIQ+NS
Sbjct: 1089 EQWTQRLVHSITEIAKSEGHCWDITIEHIERVKWYAPHIRHLVADVQCKRIQRNS 1106

BLAST of MC01g1330 vs. ExPASy TrEMBL
Match: A0A5D3CPE7 (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006500 PE=4 SV=1)

HSP 1 Score: 1754 bits (4542), Expect = 0.0
Identity = 879/1080 (81.39%), Postives = 953/1080 (88.24%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLN+HPSYFTTSSCSGRISIL+QP
Sbjct: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNSHPSYFTTSSCSGRISILAQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTNGELSEIVFRFEPLIIA 120
            I++ SA A KPKKKALGGSWL V+HEFA+P+SV+DLLFRSPSTN ELSE+VFRFEPLIIA
Sbjct: 61   ISTTSAAAPKPKKKALGGSWLFVSHEFAEPNSVIDLLFRSPSTNRELSELVFRFEPLIIA 120

Query: 121  VECKDLGSAQALVSSAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTTEKIMVSPEYV 180
            VECKDLGSAQALVS+AISCGFRESGITSASKRVIIAIRCSIRMEVPLGT+EKIMV+PEYV
Sbjct: 121  VECKDLGSAQALVSTAISCGFRESGITSASKRVIIAIRCSIRMEVPLGTSEKIMVTPEYV 180

Query: 181  RYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDSVDSKGH 240
            +Y+V+VANEKM ANKKRTDGF K +QSS+S   +  +TCHGI SREA  DVNDS+D KGH
Sbjct: 181  QYIVNVANEKMVANKKRTDGFLKALQSSIS---DALRTCHGIPSREATEDVNDSLDRKGH 240

Query: 241  VCADEEDDGAALEGGDRNA-TGSSEACSHSLSVEQMDIVGEPMAKLFIWGHSATTIHNKV 300
             CAD+ +DGAALEGG  NA +GSSEACS+SLSVE +DI GEP+ KLFIWGHSATTIHNKV
Sbjct: 241  GCADD-NDGAALEGGVWNANSGSSEACSYSLSVEPIDIAGEPIEKLFIWGHSATTIHNKV 300

Query: 301  IVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSLVGDFIYVIGGRTD 360
            I FGGFGGMGRHARRNDLLQLDM S +LQTINVED P PRLGHTSSLVGD +YV+GGRTD
Sbjct: 301  IAFGGFGGMGRHARRNDLLQLDMLSYTLQTINVEDRPFPRLGHTSSLVGDRLYVVGGRTD 360

Query: 361  PTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIV 420
            PTCIL DVW+FN T EKWTLLECTGSPFSPRHRHAAA +GSKIYVFGGLENDRISSS I 
Sbjct: 361  PTCILKDVWLFNITQEKWTLLECTGSPFSPRHRHAAAVLGSKIYVFGGLENDRISSSFIF 420

Query: 421  LDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGDLYTFDADACFWKK 480
            LD+DSH WKEIQAGGE PCGRHSHSMVS GSHIYMFGGYDGEKTLGDLY+FD +AC+WKK
Sbjct: 421  LDSDSHHWKEIQAGGEQPCGRHSHSMVSYGSHIYMFGGYDGEKTLGDLYSFDTNACYWKK 480

Query: 481  ENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHSWTHVSLKCIGKGL 540
            ENIAG TPNARFSHAMFVYKNYIGIIGGCPVTQT Q+L LLDL L  W HVSL CIG+ L
Sbjct: 481  ENIAGITPNARFSHAMFVYKNYIGIIGGCPVTQTYQELVLLDLQLRCWRHVSLNCIGREL 540

Query: 541  FVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVILGHPETIEKVANHL 600
            FVRST SVVGDD++L+GGGASCYAFGT FSEPMKI LHPL+SSEVILGH E  EK     
Sbjct: 541  FVRSTVSVVGDDLILVGGGASCYAFGTTFSEPMKISLHPLISSEVILGHSENTEK----- 600

Query: 601  IEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALYWILQIEKKYAKLG 660
               LE++NRDPEC+ NGNAQSFNEA  F I+ EK N HEQKQ GALYW+LQIE+KYAKL 
Sbjct: 601  ---LEKENRDPECIPNGNAQSFNEAFSFNIDIEKSNSHEQKQ-GALYWVLQIERKYAKLV 660

Query: 661  KDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGDQIYQKNDFHVSEL 720
            KDILK FGWLD+GR VSSR SG THICFPVN KFCD FDEKQSWW DQ  Q+NDF +S  
Sbjct: 661  KDILKKFGWLDMGRNVSSRGSG-THICFPVNGKFCDTFDEKQSWWADQFEQENDFSISGP 720

Query: 721  VSCEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKMSEAVYSLIKHNGLS 780
             S EG      S L+ALN+LKKC ATKLVDEVV+IKTA K+P+KKMSEAV SL+KH GLS
Sbjct: 721  ESWEGCLT---SNLKALNVLKKCGATKLVDEVVDIKTAAKTPFKKMSEAVSSLLKHYGLS 780

Query: 781  EELLEELPTR-LDMILLRYHLLKVRSIG----FLRWERLGDIVVLPVTSFKDPIWDTVGE 840
            E LLEELPTR  DM +L     K  SI      LRWERLGD+VVLP+TSFKDP WDT+GE
Sbjct: 781  EVLLEELPTRWFDMAILIVEKGKNLSIPNIMLALRWERLGDLVVLPLTSFKDPTWDTIGE 840

Query: 841  ELWPMIAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYSFDATKCMFS 900
            ELWP++AKSL T RLARQGRVASTGTRDSNLEILVGDNGWV+HRENGI YSFDATKCMFS
Sbjct: 841  ELWPLVAKSLGTYRLARQGRVASTGTRDSNLEILVGDNGWVEHRENGITYSFDATKCMFS 900

Query: 901  WGNLSEKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNL 960
            WGNLSEKLRMAHL+CKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNL
Sbjct: 901  WGNLSEKLRMAHLNCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPHAIEALKRNL 960

Query: 961  QANCVSNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEGGMLHVHDNV 1020
            QANCVSNRC++LEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVR LRSEGG LHVH NV
Sbjct: 961  QANCVSNRCVVLEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRALRSEGGTLHVHGNV 1020

Query: 1021 KDSEEELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADVKCRRIQQNS 1074
            KDSEEE WTR L+ SI EIA+SEGH WD+S+EHV+RVKWYAPHIRHLVADV+C+RIQQN+
Sbjct: 1021 KDSEEEQWTRRLIHSITEIAKSEGHCWDISVEHVQRVKWYAPHIRHLVADVQCKRIQQNN 1063

BLAST of MC01g1330 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 611/1083 (56.42%), Postives = 755/1083 (69.71%), Query Frame = 0

Query: 1    MEFEKRKAATMASLGSTETDKSPKGSLDTPIIPLINTLNTHPSYFTTSSCSGRISILSQP 60
            M+FEKRKAAT+AS+ S+ TDKSPKG LD PIIPL+ T+N HPSYFTTSSCSGRISILSQP
Sbjct: 1    MDFEKRKAATLASIRSSVTDKSPKGFLDEPIIPLLETINHHPSYFTTSSCSGRISILSQP 60

Query: 61   ITSASAVAVKPKKKALGGSWLLVTHEFADPDSVVDLLFRSPSTN----GELSEIVFRFEP 120
               ++      KKKA GGSWL +TH+ AD D V+ LLF S S       + SE+VFRFEP
Sbjct: 61   KPKSND---STKKKARGGSWLYITHDPADSDLVISLLFPSKSNQIDPIDQPSELVFRFEP 120

Query: 121  LIIAVECKDLGSAQALVSSAISCGFRESGITSA--SKRVIIAIRCSIRMEVPLGTTEKIM 180
            LIIAVECKDLGSAQ LV+ AIS GFRESGITS    KRVIIAIRCSIRMEVP+G TEK+M
Sbjct: 121  LIIAVECKDLGSAQFLVALAISAGFRESGITSCGDGKRVIIAIRCSIRMEVPIGDTEKLM 180

Query: 181  VSPEYVRYLVDVANEKMEANKKRTDGFFKTMQSSVSVVSNVSQTCHGILSREAMGDVNDS 240
            VSPEYV++LVD+ANEKM+AN+KRTDGF      SV++ SN  +            D ND 
Sbjct: 181  VSPEYVKFLVDIANEKMDANRKRTDGF------SVALASNGFKN----------PDEND- 240

Query: 241  VDSKGHVCADEEDDGAALEGGDRNATGSSE----ACSHSLSVEQMDIVGEPMAKLFIWGH 300
                     DE+D+   L     ++  +           + +E++ IVGEP+ KL +WGH
Sbjct: 241  --------VDEDDNYENLAANHDSSINNGNLYPGVQKELIPLEKLSIVGEPVEKLHLWGH 300

Query: 301  SATTI----HNKVIVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVEDSPSPRLGHTSSL 360
            SA TI      +VIVFGGFGG GRHARRN+ L L+ S  +L+ I V +SPS RLGHT+S+
Sbjct: 301  SACTIDESDRKEVIVFGGFGGFGRHARRNESLLLNPSCGTLKLIAVNESPSARLGHTASM 360

Query: 361  VGDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRHAAAAVGSKIYVFG 420
            VGDF++VIGGR DP  ILNDVW  + +  +W+   C GS F PRHRHAAA+VG+K+Y+FG
Sbjct: 361  VGDFMFVIGGRADPLNILNDVWRLDISTGEWSSQRCVGSEFPPRHRHAAASVGTKVYIFG 420

Query: 421  GLENDRISSSLIVLDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIYMFGGYDGEKTLGD 480
            GL ND+I SS+ +LDT   QWKE++  G+ PC RHSH+MV+ GS  +MFGGY+GE  L D
Sbjct: 421  GLYNDKIVSSMHILDTKDLQWKEVEQQGQWPCARHSHAMVAYGSQSFMFGGYNGENVLND 480

Query: 481  LYTFDADACFWKKENIAGRTPNARFSHAMFVYKNYIGIIGGCPVTQTCQQLSLLDLHLHS 540
            LY+FD  +C WK E I+G+ P+ARFSH+MFVYK+ IGIIGGCPV+Q CQ+L+LLDL    
Sbjct: 481  LYSFDVQSCSWKLEVISGKWPHARFSHSMFVYKHTIGIIGGCPVSQNCQELTLLDLKHRL 540

Query: 541  WTHVSLKCIGKGLFVRSTASVVGDDVVLIGGGASCYAFGTMFSEPMKIRLHPLMSSEVIL 600
            W  V L+ + K LFVRSTAS++GDD+++IGGGA+CYAFGT FSEP+KI L          
Sbjct: 541  WRSVRLEFMNKELFVRSTASILGDDLIVIGGGAACYAFGTKFSEPVKINL---------- 600

Query: 601  GHPETIEKVANHLIEELEQKNRDPECLLNGNAQSFNEASGFTIEREKPNLHEQKQQGALY 660
               +++    NHL  + E  + +       NA    E S       +P            
Sbjct: 601  --VQSVTMSENHLPPQPEDVSLESN---KNNADLKTETS-----LSQP------------ 660

Query: 661  WILQIEKKYAKLGKDILKTFGWLDLGRKVSSRSSGGTHICFPVNSKFCDIFDEKQSWWGD 720
            W++Q+E+KYAK GKDILK+FGWLDL RKV S    G  ICFPV   F ++F EKQ     
Sbjct: 661  WVIQLERKYAKFGKDILKSFGWLDLERKVYSNEK-GLCICFPVTENFSELFHEKQ----- 720

Query: 721  QIYQKNDFHVSELVS-CEGFRPNKCSYLEALNMLKKCDATKLVDEVVEIKTATKSPYKKM 780
                  DF  SE  +  +G      S   ALN+LK+  A KL++   E K   KSP ++M
Sbjct: 721  --LLGKDFERSEENNLTKGLSLKDISCSAALNLLKEHGAKKLINVAFEAKKVAKSPLQRM 780

Query: 781  SEAVYSLIKHNGLSEELLEELPTRLDMILLRYHLLKVRSIGFLRWERLGDIVVLPVTSFK 840
             E + S++K  GL EELL+ELP                     +WERLGDIVV+P TSFK
Sbjct: 781  REDITSILKQKGLPEELLDELPQ--------------------KWERLGDIVVVPATSFK 840

Query: 841  DPIWDTVGEELWPMIAKSLCTSRLARQGRVASTGTRDSNLEILVGDNGWVDHRENGIAYS 900
            DP W ++ +E+W  ++KSL  +RLARQGRV   GTRDS LEILVGDNGWV+HRENGI YS
Sbjct: 841  DPTWSSINDEVWCAVSKSLSANRLARQGRVEPNGTRDSTLEILVGDNGWVNHRENGILYS 900

Query: 901  FDATKCMFSWGNLSEKLRMAHLDCKEETIVDLFAGIGYFVLPFLVGAKAKLVYACEWNPH 960
            FDATKCMFSWGNLSEKLRM ++ C+ E +VDLFAGIGYFVLPFLV AKAKLVYACEWNPH
Sbjct: 901  FDATKCMFSWGNLSEKLRMGNMACENEVVVDLFAGIGYFVLPFLVRAKAKLVYACEWNPH 960

Query: 961  AIEALKRNLQANCVSNRCIILEGDNRETAPKGVADRVCLGLLPTSEGSWVTAVRTLRSEG 1020
            AIEAL+RN++AN VS RCIILEGDNR TAPKGVADRV LGL+P+SEGSWVTA++ LR EG
Sbjct: 961  AIEALRRNVEANSVSERCIILEGDNRITAPKGVADRVNLGLIPSSEGSWVTAIQALRPEG 995

Query: 1021 GMLHVHDNVKDSEEELWTRHLLQSIAEIARSEGHHWDVSIEHVERVKWYAPHIRHLVADV 1069
            G+LHVH NVKDS+E  W  H+ +++++IAR+EG  W+V++EH+E+VKWYAP IRHLVADV
Sbjct: 1021 GILHVHGNVKDSDESSWGEHVTKTLSDIARAEGRSWEVTVEHIEKVKWYAPRIRHLVADV 995

BLAST of MC01g1330 vs. TAIR 10
Match: AT1G18610.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 123.6 bits (309), Expect = 9.4e-28
Identity = 64/180 (35.56%), Postives = 88/180 (48.89%), Query Frame = 0

Query: 336 PSPRLGHTSSLV--GDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLLECTGSPFSPRHRH 395
           P  R GHT + +  G F+YV GG     C  N V VF+   + WT     G+P  PR  H
Sbjct: 22  PGKRWGHTCNAIKGGSFLYVFGGYGRDNCQTNQVHVFDAAKQIWTQPMINGTPPPPRDSH 81

Query: 396 AAAAVGSKIYVFGGLENDRISSSLIVLDTDSHQWKEIQAGGEHPCGRHSHSMVSCGSHIY 455
           +   VG  ++VFGG +       L +LDT SH WK     GE P  R  HS    G  ++
Sbjct: 82  SCTTVGDNLFVFGGTDGVNPLKDLYILDTSSHTWKCPSVRGEGPEAREGHSATLVGKRLF 141

Query: 456 MFG------GYDGEKTLGDLYTFDADACFWKKENIAGRTPNARFSHAMFVYKNYIGIIGG 508
           +FG      G + E    D+Y F+ +   WK+    G  P+AR SH+   +KN + +IGG
Sbjct: 142 VFGGCGKSSGINEEIYYNDVYIFNTETFVWKRAVTIGNPPSARDSHSCSSWKNKLVVIGG 201

BLAST of MC01g1330 vs. TAIR 10
Match: AT1G74150.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 1.2e-27
Identity = 68/210 (32.38%), Postives = 101/210 (48.10%), Query Frame = 0

Query: 327 LQTINVEDS----PSPRLGHTSSLV--GDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLL 386
           +Q + + DS    P  R GHT + +  G F+YV GG     C+ N V VF+   + W   
Sbjct: 6   VQQVGLGDSSSFGPGKRWGHTCNAIKGGRFLYVFGGFGRDNCLTNQVHVFDAETQIWIRP 65

Query: 387 ECTGSPFSPRHRHAAAAVGSKIYVFGGLENDRISSSLIVLDTDSHQWKEIQAGGEHPCGR 446
           E  G P  PR  H+   VG  ++VFGG +  +  + + +LDT SH W      GE P  R
Sbjct: 66  EINGVPPCPRDSHSCTTVGDNLFVFGGTDGTKYLNDVHILDTYSHTWIRPDIRGEGPRVR 125

Query: 447 HSHSMVSCGSHIYMFGG------YDGEKTLGDLYTFDADACFWKKENIAGRTPNARFSHA 506
            +HS       +++FGG       D E    DLY  + +   WK+   +G+ P+AR SH 
Sbjct: 126 EAHSAALVDKRLFIFGGCGKSSDSDDEVFYNDLYILNTETYMWKRAVTSGKPPSARDSHT 185

Query: 507 MFVYKNYIGIIGGCPVTQTCQQLSLLDLHL 525
              +KN I ++GG    +      L D+H+
Sbjct: 186 CSAWKNKIIVVGG----EDLDDYYLSDVHI 211

BLAST of MC01g1330 vs. TAIR 10
Match: AT5G18590.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 2.4e-23
Identity = 71/227 (31.28%), Postives = 103/227 (45.37%), Query Frame = 0

Query: 275 MDIVGEPMAKLFIWGHSATTIHNKVIVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVED 334
           + + GE  A  F   H+A TI NK+IV GG  G G     +D+  L+  SC+  T + + 
Sbjct: 69  LSVNGEKPAPRF--NHAAATIGNKMIVVGGESGSG---LLDDVQVLNFDSCTWSTASSKV 128

Query: 335 SPSPR---------LGHTSSLVGDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLLECTGS 394
             SP           GH     G  + ++GG+TDP+     VW F+T  E W+L++  G 
Sbjct: 129 YLSPSSLPLMIPAWKGHCLVSWGKKVLLVGGKTDPSSDRVSVWAFDTDSECWSLMDAKGD 188

Query: 395 PFSPRHRHAAAAVGSKIYVFGGLEN-DRISSSLIVLDTDSHQWKEIQAGGEHPCGRHSHS 454
               R  H      S + +FGG ++  R  + L + D  S  W  +   G  PC R  H 
Sbjct: 189 LPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSHHV 248

Query: 455 MVSCGSHI-YMFGGYDGEKTLGDLYTFDADACFWKKENIAGRTPNAR 491
                  I ++FGG    KTL DLY+ D +   W +  I G  P+ R
Sbjct: 249 ATLFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGFHPSPR 290

BLAST of MC01g1330 vs. TAIR 10
Match: AT5G18590.2 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 2.4e-23
Identity = 71/227 (31.28%), Postives = 103/227 (45.37%), Query Frame = 0

Query: 275 MDIVGEPMAKLFIWGHSATTIHNKVIVFGGFGGMGRHARRNDLLQLDMSSCSLQTINVED 334
           + + GE  A  F   H+A TI NK+IV GG  G G     +D+  L+  SC+  T + + 
Sbjct: 69  LSVNGEKPAPRF--NHAAATIGNKMIVVGGESGSG---LLDDVQVLNFDSCTWSTASSKV 128

Query: 335 SPSPR---------LGHTSSLVGDFIYVIGGRTDPTCILNDVWVFNTTLEKWTLLECTGS 394
             SP           GH     G  + ++GG+TDP+     VW F+T  E W+L++  G 
Sbjct: 129 YLSPSSLPLMIPAWKGHCLVSWGKKVLLVGGKTDPSSDRVSVWAFDTDSECWSLMDAKGD 188

Query: 395 PFSPRHRHAAAAVGSKIYVFGGLEN-DRISSSLIVLDTDSHQWKEIQAGGEHPCGRHSHS 454
               R  H      S + +FGG ++  R  + L + D  S  W  +   G  PC R  H 
Sbjct: 189 LPVSRSGHTVVRASSVLILFGGEDSKKRKLNDLHMFDLKSSTWLPLNCTGTRPCARSHHV 248

Query: 455 MVSCGSHI-YMFGGYDGEKTLGDLYTFDADACFWKKENIAGRTPNAR 491
                  I ++FGG    KTL DLY+ D +   W +  I G  P+ R
Sbjct: 249 ATLFDDKILFVFGGSGKNKTLNDLYSLDFETMVWSRIKIRGFHPSPR 290