MC01g1251 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g1251 vs. ExPASy Swiss-Prot
Match: Q0WQX9 (Probable E3 ubiquitin-protein ligase WAVH2 OS=Arabidopsis thaliana OX=3702 GN=WAVH2 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.0e-27
Identity = 123/469 (26.23%), Postives = 211/469 (44.99%), Query Frame = 0

Query: 74  NLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSVTCPICRAQW 133
           N  + + CAICL  +N  +  S    AIFTA+CSH+FH +C++         CP C A W
Sbjct: 133 NKSNSSRCAICLQRVN--SNQSNSTAAIFTAECSHSFHLSCVNG---LEDKRCPFCSAAW 192

Query: 134 TQLPRNSVPPSSQ---SDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDHTPTHHS 193
              P+++ P  +    SDPI R     I T +  R        Y+DD+P+   ++P   +
Sbjct: 193 NHAPKSNYPAVNNNFGSDPIRRPEIREIKTGKSLR-------VYNDDEPLA--YSPVSLA 252

Query: 194 RLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQTP 253
           +++ ++H S  +      V      PG+   +              S S  L+   L   
Sbjct: 253 QIN-TIHESDEN----DDVEDDDDFPGFFRDSSITSD------MVPSISGNLEVKLLPES 312

Query: 254 RISGNPRNRARAYLSVKL--------------AHQRATDLVLV--VCSNGPHLRLLKQAM 313
            +    + +    + +KL              A + + DLV V  + + G +L+ +K AM
Sbjct: 313 AVVETGKKKETHVVIMKLKASPSPSSITDAIKARRPSIDLVTVLDLSNGGANLQTVKHAM 372

Query: 314 ALVVSSLRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAV----- 373
             V+S LR +DRL+IV +S+ + R+  LRRMT+ G+R+A +++D L  M     V     
Sbjct: 373 RSVISLLREMDRLSIVVFSTGSKRLMPLRRMTAKGRRSARRMVDALGGMETTGGVGMSVN 432

Query: 374 EGLKKAIKILEDSTHKNPNSSILHLSDTRTQPYHLID----------------FESPAIP 433
           + LKKA+K++ED   KNP++SI  LSD + QP  ++                 F  P IP
Sbjct: 433 DALKKAVKVVEDRREKNPSTSIFVLSDGQDQPEAVLKAKLNATRIPFVVSTTRFSRPEIP 492

Query: 434 IHWFHVGMGLSPSTGCVMQEFEEFLVTKVLRGIIRDIQLRIG------------------ 479
           +H  ++    +     +   F E  +  +L   + +++L +                   
Sbjct: 493 VHSVYIASPGALLHAPLRDAFTE-RIASLLNVTLHNVKLNLSLVNGSHLTEISSVYSLTG 552

BLAST of MC01g1251 vs. ExPASy Swiss-Prot
Match: F4JSV3 (Probable E3 ubiquitin-protein ligase EDA40 OS=Arabidopsis thaliana OX=3702 GN=EDA40 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 8.3e-25
Identity = 114/444 (25.68%), Postives = 199/444 (44.82%), Query Frame = 0

Query: 77  SKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNV--RHGSVTCPICRAQWT 136
           S + C ICL     ++  +  G AIFTA+CSH FHF C++S    R+    CP+C A W 
Sbjct: 165 SSSKCGICL-----QSAKAGRGTAIFTAECSHTFHFPCVASRAGDRNLLSDCPVCGASWR 224

Query: 137 QLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDHTPTHHSRLSF 196
           +    S+ P S S  +     +S +  R ++ +      Y+DD+P+      +  SR  F
Sbjct: 225 E---TSLLPLSLSSSLHESGSESDSKIRESKNNNKSLRVYNDDEPL----ISSPISRTGF 284

Query: 197 SLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQTPRISG 256
             ++ P S     +           Y N  +     +  +      +    S +   ++ 
Sbjct: 285 --NTIPESNEDEEEEDNDDGEFKGFYVNTPSPLTTKKMLTDSVTGHVDVKLSSEAAIVAV 344

Query: 257 NPRNRARAYL------SVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRSIDRLA 316
              N   + L      S+  A +   DLV V+  +G ++ ++K+AM  V+SSLR  DRL+
Sbjct: 345 GRGNETYSVLMKIKSPSLPTARRSPVDLVTVIDVSGGNIEMVKRAMRQVISSLRETDRLS 404

Query: 317 IVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAV-EGLKKAIKILEDSTHKNP 376
           +V++SS++ R+  LRRMT+ G+R A +++D +   G   +V + +KKA K++ED   KN 
Sbjct: 405 MVSFSSSSKRLTPLRRMTANGRRLARRIVDDISGDGDGMSVNDAVKKAAKVIEDRRQKNL 464

Query: 377 NSSILHLSDTRTQPYHLIDFESP-----------AIPIHWFHVGMGLSPSTGCVMQEFEE 436
            ++I  L+D      H      P            IP H   +G         + ++   
Sbjct: 465 FTTIFVLTDRNRNSAHQAQLAQPDFVTSTRFSHLEIPTHTIWLG----ACNHALPEDVFA 524

Query: 437 FLVTKVLRGIIRDIQLRIGEESNR-------------------SSIIRIGELRGDEEKRI 479
             +  +L   ++D+ L +G  S                     S +IR+G++ GDEE+ +
Sbjct: 525 KRIKSLLSLSVQDLTLNLGLVSGSGQGKVTSVYSLSGRPVWLGSGLIRLGDMYGDEEREV 584

BLAST of MC01g1251 vs. ExPASy Swiss-Prot
Match: Q9LTA6 (E3 ubiquitin-protein ligase WAV3 OS=Arabidopsis thaliana OX=3702 GN=WAV3 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.1e-24
Identity = 125/457 (27.35%), Postives = 198/457 (43.33%), Query Frame = 0

Query: 73  NNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR-HGSVTCPICRA 132
           N+   ++ C ICL+ +      +  G A +TA+CSHAFHF CI+  VR  G + CP+C +
Sbjct: 114 NSFKFRSTCGICLNSVK-----TGQGTAKYTAECSHAFHFPCIADYVRKQGKLVCPVCNS 173

Query: 133 QWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGR-------------YDDDD 192
            W          ++   P    LDDS++  +  R     S R             YDDD+
Sbjct: 174 IWKDASLLVPHKNATESP----LDDSVSVIQEKRVVVTSSPRAKPRPKQSDYSRFYDDDE 233

Query: 193 PVEPDH---TPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSC 252
           P+        P              P F       G    P   ++    + P + +   
Sbjct: 234 PLLSPRFVTIPEADENCGGEEEDDVPQF------KGFVVDPNPSFAVKTNEIPVNGRDFG 293

Query: 253 RSPSSLLQSPSLQTP-----------RISGNPRNRARAYLSVKL--AHQRA-TDLVLVV- 312
               SLL   ++ +            R+   P   AR  +  +L    QRA  DLV+VV 
Sbjct: 294 NVQVSLLPEAAVVSVGCGYETRAVALRVKAPPPLTARGGVGRRLLDPSQRAPVDLVVVVD 353

Query: 313 ---CSNGPHLRLLKQAMALVVSSLRSIDRLAIV-TYSSAATRVFSLRRMTSYGKRAALQV 372
                NG  L+++K+AM LV+SSL S DRL+IV    +   R+  L+RMT +GKR+A  V
Sbjct: 354 VGGTMNGAKLQMVKRAMRLVISSLGSADRLSIVAVVMTVPKRLLPLKRMTEHGKRSAGAV 413

Query: 373 IDRLFYMGQADAVEGLKKAIKILEDSTHKNPNSSILHLSDTRTQPYHLIDFESPAI---- 432
           +D L     ++  E LKKA ++LED   +NP +SI+ L+D + Q   +   +   I    
Sbjct: 414 VDGLLCGQGSNTSEALKKASRVLEDRRERNPVASIVLLTDGQGQLSKVHTNQRSTITNVG 473

Query: 433 PIHWFHVGM-----GLSPSTGCVMQEFEEFL---VTKVLRGIIRD--IQLRIGE------ 461
              + H+ +     G   S GC     EE     +  +L  +++D  IQ+R+G       
Sbjct: 474 STRFAHIEIPVTEHGFGESGGCSNAPAEEAFAKCIGGLLSVVVQDLRIQIRVGSGSGPCE 533

BLAST of MC01g1251 vs. ExPASy Swiss-Prot
Match: Q9ZQ46 (E3 ubiquitin-protein ligase WAVH1 OS=Arabidopsis thaliana OX=3702 GN=WAVH1 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.6e-23
Identity = 116/472 (24.58%), Postives = 208/472 (44.07%), Query Frame = 0

Query: 77  SKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNV---RHGSVTCPICRAQW 136
           S N C ICL  +      S  G AIFTA+CSH FHF C++S      +   +CP+C +  
Sbjct: 126 SSNKCGICLQSVK-----SGQGTAIFTAECSHTFHFPCVTSRAAANHNRLASCPVCGSSL 185

Query: 137 TQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDHTPTHHSRLS 196
               RN   P SQ  P   I + S+  +             DD+  +    +P     + 
Sbjct: 186 LPEIRNYAKPESQIKP--EIKNKSLRVYN------------DDEALISSPISPAGFHTIL 245

Query: 197 FSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQTPRIS 256
            S  +     +    V+  +     L ++         + +  +     ++ S+   ++ 
Sbjct: 246 ESDENEDCEEFTGFSVNTPSPLTAKLLTDRNVDVKLSPESAIVASGKGYETYSV-VMKVK 305

Query: 257 GNPRNRARAYLSVKLAHQRATDLVLVV----CSNGPHLRLLKQAMALVVSSLRSIDRLAI 316
             P   AR +     A +   DLV V+     ++G  L +LKQ M +V+S+LR +DRL+I
Sbjct: 306 SPPFPTARGF-----ARRVPVDLVAVLDVSGRNSGGKLEMLKQTMRIVLSNLREMDRLSI 365

Query: 317 VTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAV------------EGLKKAIK 376
           + +SS++ R+  LRRMT+ G+R+A +++D +   G    V            + LKKA+K
Sbjct: 366 IAFSSSSKRLSPLRRMTANGRRSARRIVDIITVPGSVSGVGIDFSGEGMSVNDALKKAVK 425

Query: 377 ILEDSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPS------TGCVM 436
           +L+D   KNP +++  L+D   Q + +       IPIH   +   +          G + 
Sbjct: 426 VLDDRRQKNPFTAVFVLTD--RQAHQVAQLAHSRIPIHTIWLSHAIPEDAFARTINGYLS 485

Query: 437 QEFEEF-----LVTKVLRGIIRDIQLRIGEES-NRSSIIRIGELRGDEEKRIIID----- 496
              ++      +V+ + +G I  +    G  +   +  IR+G++  +EE+ ++++     
Sbjct: 486 LSVQDLGLQLGIVSGLGQGEITSVYSLSGRPAWLGTGSIRLGDMYAEEERALLVEIKSPV 545

Query: 497 -SQLSDSEGH--VRVRYSYVDGAV----NDRECTKTGETLLSLGNKSNINMT 506
            + L+ S  H  + VR  YVD       N  +      T L++ + SN N++
Sbjct: 546 NNSLTGSRSHKIMTVRSRYVDPTTQELRNPEDRALLIPTPLTVRSSSNPNIS 570

BLAST of MC01g1251 vs. NCBI nr
Match: XP_022132381.1 (uncharacterized protein LOC111005253 [Momordica charantia])

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MAGGGGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGE 60
           MAGGGGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGE
Sbjct: 1   MAGGGGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGE 60

Query: 61  AEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR 120
           AEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR
Sbjct: 61  AEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR 120

Query: 121 HGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPV 180
           HGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPV
Sbjct: 121 HGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPV 180

Query: 181 EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS 240
           EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS
Sbjct: 181 EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS 240

Query: 241 LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSS 300
           LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSS
Sbjct: 241 LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSS 300

Query: 301 LRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILE 360
           LRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILE
Sbjct: 301 LRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILE 360

Query: 361 DSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVT 420
           DSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVT
Sbjct: 361 DSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVT 420

Query: 421 KVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGA 480
           KVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGA
Sbjct: 421 KVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGA 480

Query: 481 VNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARR 540
           VNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARR
Sbjct: 481 VNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARR 540

Query: 541 WAKHLHSYRL 550
           WAKHLHSYRL
Sbjct: 541 WAKHLHSYRL 550

BLAST of MC01g1251 vs. NCBI nr
Match: XP_038883712.1 (probable E3 ubiquitin-protein ligase WAVH2 [Benincasa hispida])

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 458/546 (83.88%), Postives = 495/546 (90.66%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGEAEIA 64
           GGGGG G SILKKAARKI+VAAYACRSFSPRK RSVS+L S+S EKP+HA+EEVGE EIA
Sbjct: 3   GGGGGRGASILKKAARKIVVAAYACRSFSPRKARSVSNLSSLSAEKPRHAVEEVGEGEIA 62

Query: 65  EQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSV 124
           E+T S T NNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGSV
Sbjct: 63  EETESITPNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGSV 122

Query: 125 TCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDH 184
           TCPICRAQWTQLPRNS P  +QSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDH
Sbjct: 123 TCPICRAQWTQLPRNSAPLCTQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDH 182

Query: 185 TPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQS 244
           TP H SRLSF +H++P SF PPIQVSGCA  PGY+YSNH  +PP  QQF CRS S LLQS
Sbjct: 183 TPAHDSRLSFYVHTTPSSFCPPIQVSGCA--PGYVYSNHHTRPPLRQQFPCRSLSPLLQS 242

Query: 245 PSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRSI 304
           P+ QT RI+GNPRN +R YLSVKLAHQ+ATDLVLVVC+NGPHLRLLKQAMALVVSSLRSI
Sbjct: 243 PARQTSRITGNPRNSSRYYLSVKLAHQQATDLVLVVCANGPHLRLLKQAMALVVSSLRSI 302

Query: 305 DRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDSTH 364
           DRLAIVTYSSAATRVF L+RMTSYGKRAALQVIDRLFYMGQ DAVEGLKKA+KILEDS H
Sbjct: 303 DRLAIVTYSSAATRVFPLKRMTSYGKRAALQVIDRLFYMGQPDAVEGLKKAVKILEDSIH 362

Query: 365 KNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVLR 424
           KNPNSSILHLSD+RTQP HLI+ +SPA+PIHWFHVG+G S STG VMQEFEEFL+TKVLR
Sbjct: 363 KNPNSSILHLSDSRTQPCHLINLDSPAVPIHWFHVGLGFSISTGFVMQEFEEFLMTKVLR 422

Query: 425 GIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVNDR 484
           GIIRDIQLRIGEE++RS+II I ELRGDEEKRI+ID+  SD+EGHVRVRYSYVDG VND 
Sbjct: 423 GIIRDIQLRIGEENSRSTIIGIAELRGDEEKRIVIDNLFSDNEGHVRVRYSYVDGEVNDG 482

Query: 485 ECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAKH 544
           EC K GETLLS+GNKS+  +TFDEAGRE+REED S GGRISS ERW+YHDPFMARRWAKH
Sbjct: 483 ECMKVGETLLSIGNKSSNIVTFDEAGRENREEDASMGGRISSVERWEYHDPFMARRWAKH 542

Query: 545 LHSYRL 550
           LHSYRL
Sbjct: 543 LHSYRL 546

BLAST of MC01g1251 vs. NCBI nr
Match: KAG6603849.1 (E3 ubiquitin-protein ligase WAVH1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034029.1 hypothetical protein SDJN02_03755 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 458/547 (83.73%), Postives = 486/547 (88.85%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSV-SVEKPKHAMEEVGEAEI 64
           GGGGG G SILKKAARKI   AYACRSFSPRK RS S   +  S EKP++ +EEVG AEI
Sbjct: 3   GGGGGRGTSILKKAARKI---AYACRSFSPRKARSASSFSAAPSAEKPRYVVEEVGVAEI 62

Query: 65  AEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 124
           AEQT SRTTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGS
Sbjct: 63  AEQTESRTTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 122

Query: 125 VTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 184
           VTCPICRAQWTQLPRNS P  +QSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD
Sbjct: 123 VTCPICRAQWTQLPRNSAPLCTQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 182

Query: 185 HTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQ 244
           HTPTH SRLSFS+HS+P SF PPIQVSGCA  PGY+ SNH A+PP  Q F CRSPS LLQ
Sbjct: 183 HTPTHDSRLSFSVHSAPSSFCPPIQVSGCA--PGYVCSNHHARPPLRQHFPCRSPSPLLQ 242

Query: 245 SPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRS 304
           SP+ QTPR +GN RNR RA+LSVKLAHQ ATDLVLV CSNGPHLRLLKQAMALVVSSLRS
Sbjct: 243 SPARQTPRTTGNLRNRMRAFLSVKLAHQPATDLVLVACSNGPHLRLLKQAMALVVSSLRS 302

Query: 305 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 364
           IDRLAIVTYSSAATRVF L+RMTSYGKRAALQVIDRLFYMGQADAVEGLKKA+KILEDS 
Sbjct: 303 IDRLAIVTYSSAATRVFPLKRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAVKILEDSI 362

Query: 365 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVL 424
           HKNPNSSILHLSD+RTQPYHLI+ ESPA+PIHWFHVGMG S S G VMQEFEEFL+TKVL
Sbjct: 363 HKNPNSSILHLSDSRTQPYHLINLESPAVPIHWFHVGMGFSISNGFVMQEFEEFLMTKVL 422

Query: 425 RGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVND 484
           RGIIRDIQLR+GEES RS+II I ELRGDEEKRI+ID+   D+EGHVRVRYSYVDG V+D
Sbjct: 423 RGIIRDIQLRVGEESGRSTIISIAELRGDEEKRIVIDNLCGDNEGHVRVRYSYVDGEVDD 482

Query: 485 RECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAK 544
           +EC K GE  LSLGNKSN N TF+EAGRE+ EED S GGRISS ERWDYHDPFMARRWAK
Sbjct: 483 QECMKAGEIFLSLGNKSNNNSTFNEAGRENSEEDPSLGGRISSIERWDYHDPFMARRWAK 542

Query: 545 HLHSYRL 550
           HLHSYRL
Sbjct: 543 HLHSYRL 544

BLAST of MC01g1251 vs. NCBI nr
Match: XP_022950608.1 (uncharacterized protein LOC111453655 [Cucurbita moschata])

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 456/547 (83.36%), Postives = 487/547 (89.03%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSV-SVEKPKHAMEEVGEAEI 64
           GGGGG G SILKKAARKI   AYACRSFSPRK RS S+  +  S EKP++ +EEVGEAEI
Sbjct: 3   GGGGGRGTSILKKAARKI---AYACRSFSPRKARSTSNFSAAPSAEKPRYVVEEVGEAEI 62

Query: 65  AEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 124
           AEQT SRTTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGS
Sbjct: 63  AEQTESRTTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 122

Query: 125 VTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 184
           VTCPICRAQWTQLPRNS P  +QSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD
Sbjct: 123 VTCPICRAQWTQLPRNSAPLCTQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 182

Query: 185 HTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQ 244
           HTP+H SRLSFS+HS+P +F PPIQVSGCA  PGY+ SNH A+PP  Q F CRSPS LLQ
Sbjct: 183 HTPSHDSRLSFSVHSAPSNFCPPIQVSGCA--PGYVCSNHHARPPLRQHFPCRSPSPLLQ 242

Query: 245 SPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRS 304
           SP+ QTPR +GN RNR RA+LSVKLAHQ ATDLVLV CSNGPHLRLLKQAMALVVSSLRS
Sbjct: 243 SPARQTPRTTGNLRNRMRAFLSVKLAHQPATDLVLVACSNGPHLRLLKQAMALVVSSLRS 302

Query: 305 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 364
           IDRLAIVTYSSAATRVF L+RMTSYGKRAALQVIDRLFYMGQADAVEGLKKA+KILEDS 
Sbjct: 303 IDRLAIVTYSSAATRVFPLKRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAVKILEDSI 362

Query: 365 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVL 424
           HKNPNSSILHLSD+RTQPYHLI+ ESPA+PIHWFHVGMG S S G VMQEFEEFL+TKVL
Sbjct: 363 HKNPNSSILHLSDSRTQPYHLINLESPAVPIHWFHVGMGFSISNGFVMQEFEEFLMTKVL 422

Query: 425 RGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVND 484
           RGIIRDIQLR+GEES RS+II I ELRGDEEKRI+ID+   D+EGHVRVRYSYVDG V+D
Sbjct: 423 RGIIRDIQLRVGEESGRSTIISIAELRGDEEKRIVIDNLCGDNEGHVRVRYSYVDGEVDD 482

Query: 485 RECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAK 544
           +EC K GE  LSLGNKSN N  F+EAGRE+ EED S GGRISS ERWDYHDPFMARRWAK
Sbjct: 483 QECMKAGEIFLSLGNKSNNNSNFNEAGRENSEEDPSLGGRISSIERWDYHDPFMARRWAK 542

Query: 545 HLHSYRL 550
           HLHSYRL
Sbjct: 543 HLHSYRL 544

BLAST of MC01g1251 vs. NCBI nr
Match: XP_023543048.1 (uncharacterized protein LOC111803031 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 454/547 (83.00%), Postives = 486/547 (88.85%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSV-SVEKPKHAMEEVGEAEI 64
           GGGGG G SILKKAARKI   AYACRSFSPRK RS S+  +  S EKP++ + EVGEAEI
Sbjct: 3   GGGGGRGTSILKKAARKI---AYACRSFSPRKARSASNFSAAPSAEKPRYVVGEVGEAEI 62

Query: 65  AEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 124
           AEQT SRTTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGS
Sbjct: 63  AEQTESRTTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 122

Query: 125 VTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 184
           VTCPICRAQWTQLPRNS P  +QSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD
Sbjct: 123 VTCPICRAQWTQLPRNSAPLCTQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 182

Query: 185 HTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQ 244
           HTPTH SRLSFS+HS+P SF PPIQVSGCA  PGY+ SNH A+PP  Q F CRSPS LLQ
Sbjct: 183 HTPTHDSRLSFSVHSAPSSFCPPIQVSGCA--PGYVCSNHHARPPLRQHFPCRSPSPLLQ 242

Query: 245 SPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRS 304
           SP+ QTPR +GN RNR RA+LSVKLAHQ ATDLVLV CSNGPHLRLLKQAMALVVSSLRS
Sbjct: 243 SPARQTPRTTGNLRNRMRAFLSVKLAHQPATDLVLVACSNGPHLRLLKQAMALVVSSLRS 302

Query: 305 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 364
           IDRLAIVTYSSAATRVF L+RMTSYGKRAALQVIDRLFYMGQADAVEGLKKA+KILEDS 
Sbjct: 303 IDRLAIVTYSSAATRVFPLKRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAVKILEDSI 362

Query: 365 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVL 424
           HKNPNSSILH+SD+RTQPYHLI+ ESPA+PIHWFHVGMG S S G VMQEFEEFL+TKVL
Sbjct: 363 HKNPNSSILHVSDSRTQPYHLINLESPAVPIHWFHVGMGFSISNGFVMQEFEEFLMTKVL 422

Query: 425 RGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVND 484
           RGIIRDIQLR+GEES RS+II I ELRGDEEKR++ID+   D EGHVRVRY+YVDG V+D
Sbjct: 423 RGIIRDIQLRVGEESGRSTIISIAELRGDEEKRVLIDNLCGDHEGHVRVRYTYVDGEVDD 482

Query: 485 RECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAK 544
           +EC K GE  LSLGNKSN N +F+EAGRE+ EED S GGRISS ERWDYHDPFMARRWAK
Sbjct: 483 QECMKAGEIFLSLGNKSNNNSSFNEAGRENSEEDPSLGGRISSIERWDYHDPFMARRWAK 542

Query: 545 HLHSYRL 550
           HLHSYRL
Sbjct: 543 HLHSYRL 544

BLAST of MC01g1251 vs. ExPASy TrEMBL
Match: A0A6J1BS39 (uncharacterized protein LOC111005253 OS=Momordica charantia OX=3673 GN=LOC111005253 PE=4 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 1   MAGGGGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGE 60
           MAGGGGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGE
Sbjct: 1   MAGGGGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGE 60

Query: 61  AEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR 120
           AEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR
Sbjct: 61  AEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVR 120

Query: 121 HGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPV 180
           HGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPV
Sbjct: 121 HGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPV 180

Query: 181 EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS 240
           EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS
Sbjct: 181 EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS 240

Query: 241 LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSS 300
           LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSS
Sbjct: 241 LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSS 300

Query: 301 LRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILE 360
           LRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILE
Sbjct: 301 LRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILE 360

Query: 361 DSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVT 420
           DSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVT
Sbjct: 361 DSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVT 420

Query: 421 KVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGA 480
           KVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGA
Sbjct: 421 KVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGA 480

Query: 481 VNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARR 540
           VNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARR
Sbjct: 481 VNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARR 540

Query: 541 WAKHLHSYRL 550
           WAKHLHSYRL
Sbjct: 541 WAKHLHSYRL 550

BLAST of MC01g1251 vs. ExPASy TrEMBL
Match: A0A6J1GFC7 (uncharacterized protein LOC111453655 OS=Cucurbita moschata OX=3662 GN=LOC111453655 PE=4 SV=1)

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 456/547 (83.36%), Postives = 487/547 (89.03%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSV-SVEKPKHAMEEVGEAEI 64
           GGGGG G SILKKAARKI   AYACRSFSPRK RS S+  +  S EKP++ +EEVGEAEI
Sbjct: 3   GGGGGRGTSILKKAARKI---AYACRSFSPRKARSTSNFSAAPSAEKPRYVVEEVGEAEI 62

Query: 65  AEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 124
           AEQT SRTTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGS
Sbjct: 63  AEQTESRTTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 122

Query: 125 VTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 184
           VTCPICRAQWTQLPRNS P  +QSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD
Sbjct: 123 VTCPICRAQWTQLPRNSAPLCTQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 182

Query: 185 HTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQ 244
           HTP+H SRLSFS+HS+P +F PPIQVSGCA  PGY+ SNH A+PP  Q F CRSPS LLQ
Sbjct: 183 HTPSHDSRLSFSVHSAPSNFCPPIQVSGCA--PGYVCSNHHARPPLRQHFPCRSPSPLLQ 242

Query: 245 SPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRS 304
           SP+ QTPR +GN RNR RA+LSVKLAHQ ATDLVLV CSNGPHLRLLKQAMALVVSSLRS
Sbjct: 243 SPARQTPRTTGNLRNRMRAFLSVKLAHQPATDLVLVACSNGPHLRLLKQAMALVVSSLRS 302

Query: 305 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 364
           IDRLAIVTYSSAATRVF L+RMTSYGKRAALQVIDRLFYMGQADAVEGLKKA+KILEDS 
Sbjct: 303 IDRLAIVTYSSAATRVFPLKRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAVKILEDSI 362

Query: 365 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVL 424
           HKNPNSSILHLSD+RTQPYHLI+ ESPA+PIHWFHVGMG S S G VMQEFEEFL+TKVL
Sbjct: 363 HKNPNSSILHLSDSRTQPYHLINLESPAVPIHWFHVGMGFSISNGFVMQEFEEFLMTKVL 422

Query: 425 RGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVND 484
           RGIIRDIQLR+GEES RS+II I ELRGDEEKRI+ID+   D+EGHVRVRYSYVDG V+D
Sbjct: 423 RGIIRDIQLRVGEESGRSTIISIAELRGDEEKRIVIDNLCGDNEGHVRVRYSYVDGEVDD 482

Query: 485 RECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAK 544
           +EC K GE  LSLGNKSN N  F+EAGRE+ EED S GGRISS ERWDYHDPFMARRWAK
Sbjct: 483 QECMKAGEIFLSLGNKSNNNSNFNEAGRENSEEDPSLGGRISSIERWDYHDPFMARRWAK 542

Query: 545 HLHSYRL 550
           HLHSYRL
Sbjct: 543 HLHSYRL 544

BLAST of MC01g1251 vs. ExPASy TrEMBL
Match: A0A6J1IUI3 (uncharacterized protein LOC111478697 OS=Cucurbita maxima OX=3661 GN=LOC111478697 PE=4 SV=1)

HSP 1 Score: 886 bits (2289), Expect = 0.0
Identity = 454/547 (83.00%), Postives = 485/547 (88.67%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSV-SVEKPKHAMEEVGEAEI 64
           GGGGG G SILKKAARKI   AYACRSFSPRK RS S+  +  S EKP++ +EEVGE+EI
Sbjct: 3   GGGGGRGTSILKKAARKI---AYACRSFSPRKARSASNFSAAPSAEKPRYVVEEVGESEI 62

Query: 65  AEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 124
           AEQT SRTTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGS
Sbjct: 63  AEQTESRTTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGS 122

Query: 125 VTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPD 184
           VTCPICRAQWTQLPRNS P  +QSDPILRILDDSIATFRINRRSFLLS RYDDDDPVEPD
Sbjct: 123 VTCPICRAQWTQLPRNSAPLCTQSDPILRILDDSIATFRINRRSFLLSARYDDDDPVEPD 182

Query: 185 HTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQ 244
           HTPTH SRLSFS+HS+P SF PP QVSGCA  PGY+ SNH A+PP  Q F CRSPS LLQ
Sbjct: 183 HTPTHDSRLSFSVHSTPSSFCPPTQVSGCA--PGYVCSNHHARPPLRQHFPCRSPSPLLQ 242

Query: 245 SPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRS 304
           SP+ QTPR +G  RNR RA+LSVKLAHQRATDLVLV CSNGPHLRLLKQAMALVVSSLRS
Sbjct: 243 SPAWQTPRTTGYLRNRMRAFLSVKLAHQRATDLVLVACSNGPHLRLLKQAMALVVSSLRS 302

Query: 305 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 364
           IDRLAIVTYSSAATRVF L+RMTSYGKRAALQVIDRLFYMGQADAVEGL+KA+KILEDS 
Sbjct: 303 IDRLAIVTYSSAATRVFPLKRMTSYGKRAALQVIDRLFYMGQADAVEGLRKAVKILEDSI 362

Query: 365 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVL 424
           HKNPNSSILHLSD+RTQPYHLI+ ESPA+PIHWFHVGMG S S G VMQEFEEFL+TKVL
Sbjct: 363 HKNPNSSILHLSDSRTQPYHLINLESPAVPIHWFHVGMGFSISNGFVMQEFEEFLMTKVL 422

Query: 425 RGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVND 484
           RGIIRDIQLR+GEES RS+II I ELRGDEEKRI+ID+  SD+EG VRVRYSYVDG V+D
Sbjct: 423 RGIIRDIQLRVGEESGRSTIISIAELRGDEEKRIVIDNLCSDNEGRVRVRYSYVDGEVDD 482

Query: 485 RECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAK 544
           +EC K GE  LSLGNKSN N T +EAGRE+ EED S GGRISS ERWDYHDPFMARRWAK
Sbjct: 483 QECMKAGEIFLSLGNKSNNNSTLNEAGRENSEEDPSLGGRISSIERWDYHDPFMARRWAK 542

Query: 545 HLHSYRL 550
           HLHSYRL
Sbjct: 543 HLHSYRL 544

BLAST of MC01g1251 vs. ExPASy TrEMBL
Match: A0A1S3B321 (uncharacterized protein LOC103485210 OS=Cucumis melo OX=3656 GN=LOC103485210 PE=4 SV=1)

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 445/546 (81.50%), Postives = 485/546 (88.83%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVSDLLSVSVEKPKHAMEEVGEAEIA 64
           GGG  G  SILKKAARKI+VAAYACRSFSPRK RSVS+L  VS EK +H +E+VGE EIA
Sbjct: 3   GGGRRGASSILKKAARKIVVAAYACRSFSPRKARSVSNLSVVSAEKSRHVVEDVGEGEIA 62

Query: 65  EQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSV 124
           +QT S TTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSHAFHFTCISSNVRHGSV
Sbjct: 63  DQTESITTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSHAFHFTCISSNVRHGSV 122

Query: 125 TCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDH 184
           TCPICRAQWTQLPRNS P   QSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVE DH
Sbjct: 123 TCPICRAQWTQLPRNSAPLCPQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVELDH 182

Query: 185 TPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQS 244
           TPTH SRLSF +H++P SF PPIQV+GC   PGY+ SNH A+PP  QQF CRSPS  LQS
Sbjct: 183 TPTHDSRLSFYVHTTPSSFCPPIQVNGCT--PGYVCSNHHARPPLRQQFLCRSPSPSLQS 242

Query: 245 PSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRSI 304
           P+ QTPR++GNPRN +R YLSVKLAHQ+ATDLVL+ C+NGPHLRLLKQAM LVVSSLRSI
Sbjct: 243 PARQTPRMTGNPRNSSRYYLSVKLAHQQATDLVLIACANGPHLRLLKQAMGLVVSSLRSI 302

Query: 305 DRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDSTH 364
           DRLAIVTYSS+ATRVF L+RMTSYGKRAALQVIDRLFYMGQ D VEGLKKA+KILEDS H
Sbjct: 303 DRLAIVTYSSSATRVFPLKRMTSYGKRAALQVIDRLFYMGQPDTVEGLKKAVKILEDSIH 362

Query: 365 KNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTGCVMQEFEEFLVTKVLR 424
           KNPNSSILHLSD+RTQPYHLI+ ESP +P+HWFHVG+G S STG VMQEFEEFL+TKVLR
Sbjct: 363 KNPNSSILHLSDSRTQPYHLINLESPVVPVHWFHVGLGFSISTGFVMQEFEEFLMTKVLR 422

Query: 425 GIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEGHVRVRYSYVDGAVNDR 484
           GIIR+IQLRIGEES+RS+II I ELRGDEEK+I+ID+  SD+EGHVRVRYSYVDG VND 
Sbjct: 423 GIIRNIQLRIGEESSRSTIIGIAELRGDEEKKIVIDNLFSDNEGHVRVRYSYVDGEVNDE 482

Query: 485 ECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDERWDYHDPFMARRWAKH 544
           EC K GET LS+GNKSN  +T DEAGRE+REED S GGRISS ERW+YHDPFMARRWAKH
Sbjct: 483 ECIKVGETQLSIGNKSNNIVTLDEAGRENREEDASMGGRISSVERWEYHDPFMARRWAKH 542

Query: 545 LHSYRL 550
           LHSYRL
Sbjct: 543 LHSYRL 546

BLAST of MC01g1251 vs. ExPASy TrEMBL
Match: A0A5D3CM12 (Zinc finger, C3HC4 type (RING finger) protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00470 PE=4 SV=1)

HSP 1 Score: 874 bits (2259), Expect = 0.0
Identity = 445/562 (79.18%), Postives = 485/562 (86.30%), Query Frame = 0

Query: 5   GGGGGGGGSILKKAARKIMVAAYACRSFSPRKVRSVS----------------DLLSVSV 64
           GGG  G  SILKKAARKI+VAAYACRSFSPRK RSVS                +L  VS 
Sbjct: 3   GGGRRGASSILKKAARKIVVAAYACRSFSPRKARSVSFFFFFFFFFFFLVYVSNLSVVSA 62

Query: 65  EKPKHAMEEVGEAEIAEQTHSRTTNNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSH 124
           EK +H +E+VGE EIA+QT S TTNNLPSKNLCAICLDPLNYKT+GSRPGQAIFTAQCSH
Sbjct: 63  EKSRHVVEDVGEGEIADQTESITTNNLPSKNLCAICLDPLNYKTKGSRPGQAIFTAQCSH 122

Query: 125 AFHFTCISSNVRHGSVTCPICRAQWTQLPRNSVPPSSQSDPILRILDDSIATFRINRRSF 184
           AFHFTCISSNVRHGSVTCPICRAQWTQLPRNS P   QSDPILRILDDSIATFRINRRSF
Sbjct: 123 AFHFTCISSNVRHGSVTCPICRAQWTQLPRNSAPLCPQSDPILRILDDSIATFRINRRSF 182

Query: 185 LLSGRYDDDDPVEPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPP 244
           LLSGRYDDDDPVE DHTPTH SRLSF +H++P SF PPIQV+GC   PGY+ SNH A+PP
Sbjct: 183 LLSGRYDDDDPVELDHTPTHDSRLSFYVHTTPSSFCPPIQVNGCT--PGYVCSNHHARPP 242

Query: 245 PHQQFSCRSPSSLLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVVCSNGPHLR 304
             QQF CRSPS  LQSP+ QTPR++GNPRN +R YLSVKLAHQ+ATDLVL+ C+NGPHLR
Sbjct: 243 LRQQFLCRSPSPSLQSPARQTPRMTGNPRNSSRYYLSVKLAHQQATDLVLIACANGPHLR 302

Query: 305 LLKQAMALVVSSLRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADA 364
           LLKQAM LVVSSLRSIDRLAIVTYSS+ATRVF L+RMTSYGKRAALQVIDRLFYMGQ D 
Sbjct: 303 LLKQAMGLVVSSLRSIDRLAIVTYSSSATRVFPLKRMTSYGKRAALQVIDRLFYMGQPDT 362

Query: 365 VEGLKKAIKILEDSTHKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFHVGMGLSPSTG 424
           VEGLKKA+KILEDS HKNPNSSILHLSD+RTQPYHLI+ ESP +P+HWFHVG+G S STG
Sbjct: 363 VEGLKKAVKILEDSIHKNPNSSILHLSDSRTQPYHLINLESPVVPVHWFHVGLGFSISTG 422

Query: 425 CVMQEFEEFLVTKVLRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLSDSEG 484
            VMQEFEEFL+TKVLRGIIR+IQLRIGEES+RS+II I ELRGDEEK+I+ID+  SD+EG
Sbjct: 423 FVMQEFEEFLMTKVLRGIIRNIQLRIGEESSRSTIIGIAELRGDEEKKIVIDNLFSDNEG 482

Query: 485 HVRVRYSYVDGAVNDRECTKTGETLLSLGNKSNINMTFDEAGREDREEDVSSGGRISSDE 544
           HVRVRYSYVDG VND EC K GET LS+GNKSN  +T DEAGRE+REED S GGRISS E
Sbjct: 483 HVRVRYSYVDGEVNDEECIKVGETQLSIGNKSNNIVTLDEAGRENREEDASMGGRISSVE 542

Query: 545 RWDYHDPFMARRWAKHLHSYRL 550
           RW+YHDPFMARRWAKHLHSYRL
Sbjct: 543 RWEYHDPFMARRWAKHLHSYRL 562

BLAST of MC01g1251 vs. TAIR 10
Match: AT5G60710.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 142.5 bits (358), Expect = 1.0e-33
Identity = 121/397 (30.48%), Postives = 177/397 (44.58%), Query Frame = 0

Query: 31  SFSPRKVRSVSDLLSVSVEKPKHAMEEVGEAEIAEQTHSRTTNNLPSKNLCAICLDPLNY 90
           S  PR+      L  V V++P           +      R+ +   SK  CAICL  +  
Sbjct: 27  SSPPRRSDDAVSLSPVIVQRPSTPTPSSSGLRL-----PRSMSKSSSKKTCAICLTAMK- 86

Query: 91  KTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSVTCPICRAQWTQLPRNS--VPPSSQSD 150
               +  G AIFTA+CSH+FHF CI++NV+HG+  CP+CRA+W ++P  S    P S   
Sbjct: 87  ----AGQGHAIFTAECSHSFHFQCITTNVKHGNQICPVCRAKWNEIPIQSPNAKPKSGVK 146

Query: 151 PILRILDDSIATFRINRRS----------FLLSGRYDDDDPVEPDHTPTHHSRLSFSLHS 210
           PI R  DD+  +    R S            +S  ++ +  V  D     H   S     
Sbjct: 147 PIGRPRDDAWMSIPPRRSSPIQYTSRPDCLRVSSIFNTEPAVFNDDEALEHQDRSAESGL 206

Query: 211 SPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQTPRISGNPRN 270
             P       V+G      Y   +   +    + F+      L+   +  + + S NP +
Sbjct: 207 DKPG------VTGTLEVKTYPEISEVVRSVSFKDFAV-----LINLKAPTSSKSSSNPSS 266

Query: 271 RARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRSIDRLAIVTYSSAATR 330
            +RA + +       T L +     G  L LLK+AM  V+ +L   DRL+++++SS A R
Sbjct: 267 SSRAPVDL------VTVLDVSGSMAGTKLALLKRAMGFVIQNLGPFDRLSVISFSSTARR 326

Query: 331 VFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDSTHKNPNSSILHLSD-- 390
            F LR MT  GK+ ALQ ++ L   G  +  EGLKK  ++L D   KNP SSI+ LSD  
Sbjct: 327 NFPLRLMTETGKQEALQAVNSLVSNGGTNIAEGLKKGARVLIDRRFKNPVSSIVLLSDGQ 386

Query: 391 ---TRTQP-------YHLI---DFESPAIPIHWFHVG 401
              T T P       Y  +   +     IP+H F  G
Sbjct: 387 DTYTMTSPNGSRGTDYKALLPKEINGNRIPVHAFGFG 396

BLAST of MC01g1251 vs. TAIR 10
Match: AT1G08050.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-30
Identity = 104/336 (30.95%), Postives = 153/336 (45.54%), Query Frame = 0

Query: 77  SKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSVTCPICRAQWTQL 136
           SK  CAICL  +         G+AIFTA+CSH+FHF CI+SNV+HG+  CP+CR QW Q+
Sbjct: 65  SKGRCAICLYEIR-----KEDGKAIFTAECSHSFHFDCITSNVKHGNRICPLCRTQWKQV 124

Query: 137 PRNSVPPSSQSDPILRILDDSIATFRINRRSFLLSGRYDDDDPVEPDHTPTHHSRLSFSL 196
           P   V              DS+ TF   R        ++DD+P+    T  H        
Sbjct: 125 PLCDV--------------DSVPTFVAQR-------GFEDDEPLPQGDTQIHSDGHRSDH 184

Query: 197 HSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQTPRISGNP 256
            +     +P +                 A+P     F+            L+   +S + 
Sbjct: 185 QALEIKLFPEVSA--------------LAKPVSRADFAV--------LVHLKAEGVSDDA 244

Query: 257 RNRARAYLSVKLAHQRATDLVLVVCSNGPHLRLLKQAMALVVSSLRSIDRLAIVTYSSAA 316
           R RARA L +       T L +    +G  + L+K AM+ V+ +L   DRL+++++SS A
Sbjct: 245 R-RARAPLDL------ITVLDVSGSMDGVKMELMKNAMSFVIQNLGETDRLSVISFSSMA 304

Query: 317 TRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDSTHKNPNSSILHLSD 376
            R+F LR M+  GK+AA+Q ++ L   G  +  EGLK   +++E    KNP S ++ LSD
Sbjct: 305 RRLFPLRLMSETGKQAAMQAVNSLVADGGTNIAEGLKIGARVIEGRRWKNPVSGMMLLSD 345

Query: 377 TRTQ------------PYHLIDFESPAIPIHWFHVG 401
            +               Y  +   S  IPIH F  G
Sbjct: 365 GQDNFTFSHAGVRLRTDYESLLPSSCRIPIHTFGFG 345

BLAST of MC01g1251 vs. TAIR 10
Match: AT2G38970.1 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-30
Identity = 103/322 (31.99%), Postives = 160/322 (49.69%), Query Frame = 0

Query: 73  NNLPSKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSVTCPICRAQ 132
           N+  S   C+IC   LN   EG   G A+FTA+CSH+FHF CI+SNV+HG+  CP+CRA+
Sbjct: 64  NSSKSSKTCSIC---LNKMKEGG--GHALFTAECSHSFHFHCIASNVKHGNQVCPVCRAK 123

Query: 133 WTQLPRNSVPPSSQSDPILRILDDSIATFRINR----RSFLLSGR--------YDDDDPV 192
           W ++P    P         R  ++  A   ++R    R  +  GR        +DDD+ +
Sbjct: 124 WKEIPMQH-PSFDLPYLFARSYNNDAAISLVHRLPRSRGVMNQGRGLAPEPSMFDDDERL 183

Query: 193 EPDHTPTHHSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSS 252
           E     +  S  S +L ++ P     +++    SA     S  +     H     R+ + 
Sbjct: 184 EQQLVFSGKS-YSDALENNHPVRMMDLKIYPEVSAVPRADSREKFDVLVH----LRAAAM 243

Query: 253 LLQSPSLQTPRISGNPRNRARAYLSVKLAHQRATDLVLVV----CSNGPHLRLLKQAMAL 312
           +  + +    +IS  PR                 DLV V+       G  L LLK+AM  
Sbjct: 244 VTGNANSLNNQISRYPR--------------APVDLVTVLDISGSMAGTKLALLKRAMGF 303

Query: 313 VVSSLRSIDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAI 372
           V+ +L S DRL+++ +SS A R+F L +M+  G++ ALQ ++ +   G  +  EGL+K +
Sbjct: 304 VIQNLGSNDRLSVIAFSSTARRLFPLTKMSDAGRQRALQAVNSVVANGGTNIAEGLRKGV 360

Query: 373 KILEDSTHKNPNSSILHLSDTR 379
           K++ED   KNP +SI+ LSD R
Sbjct: 364 KVMEDRRDKNPVASIILLSDGR 360

BLAST of MC01g1251 vs. TAIR 10
Match: AT3G54780.3 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.8e-30
Identity = 121/420 (28.81%), Postives = 196/420 (46.67%), Query Frame = 0

Query: 77  SKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSVTCPICRAQWTQL 136
           SK  C+IC   LN   EG   G AIFTA+CSH FHF CI+SNV+HG+  CP+CRA+W ++
Sbjct: 71  SKQTCSIC---LNKMKEGC--GHAIFTAECSHMFHFHCIASNVKHGNQVCPVCRAKWKEI 130

Query: 137 PRNSVPPSSQSDPILRILDD-SIATFRI---NRRSFLLS----GRYDDDDPVEPDHTPTH 196
           P           P  R  +D +I+ FR    ++R+          +DDD+ +E       
Sbjct: 131 PIQKPSLDLPYYPFDRCNNDAAISLFRCLPPSQRAITQGHPEPATFDDDERLEEQIVFDG 190

Query: 197 HSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQ 256
            + +   L      +   + +         +Y    A P   Q  SC +   L+   ++ 
Sbjct: 191 ETEV---LKKENRDYVRMMDMK--------VYPEVSAVP---QSKSCENFDVLVHLKAVT 250

Query: 257 TPRISGNPRNRARAYLSVKLAHQRA-TDLVLVV----CSNGPHLRLLKQAMALVVSSLRS 316
             +IS                ++RA  DLV V+       G  L LLK+AM  V+ +L S
Sbjct: 251 GDQIS---------------QYRRAPIDLVTVLDISGSMGGTKLALLKRAMGFVIQNLGS 310

Query: 317 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 376
            DRL+++ +SS A R+F L RM+  G++ ALQ ++ L   G  + V+GL+K  K++ED  
Sbjct: 311 SDRLSVIAFSSTARRLFPLTRMSDAGRQLALQAVNSLVANGGTNIVDGLRKGAKVMEDRL 370

Query: 377 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFH-VGMGLSPSTGCVMQEFEEFLVTKV 436
            +N  +SI+ LSD R    +  +   P+        +G  LS +   +  E E       
Sbjct: 371 ERNSVASIILLSDGRDT--YTTNHPDPSYKDALAQCIGGLLSVAVQELRVEIEGVSPNVR 430

Query: 437 LRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLS-DSEGH---VRVRYSYVD 479
           L  I       +      S ++ +G+L  DEE+  ++   +  + +GH   +++R  Y++
Sbjct: 431 LSSIKAGSYSSLVTGDGHSGLVDLGDLYADEERDFLVSINIPVEEDGHTPLLKLRCLYIN 454

BLAST of MC01g1251 vs. TAIR 10
Match: AT3G54780.2 (Zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 129.8 bits (325), Expect = 6.7e-30
Identity = 120/420 (28.57%), Postives = 195/420 (46.43%), Query Frame = 0

Query: 77  SKNLCAICLDPLNYKTEGSRPGQAIFTAQCSHAFHFTCISSNVRHGSVTCPICRAQWTQL 136
           S   C+IC   LN   EG   G AIFTA+CSH FHF CI+SNV+HG+  CP+CRA+W ++
Sbjct: 70  SSKTCSIC---LNKMKEGC--GHAIFTAECSHMFHFHCIASNVKHGNQVCPVCRAKWKEI 129

Query: 137 PRNSVPPSSQSDPILRILDD-SIATFRI---NRRSFLLS----GRYDDDDPVEPDHTPTH 196
           P           P  R  +D +I+ FR    ++R+          +DDD+ +E       
Sbjct: 130 PIQKPSLDLPYYPFDRCNNDAAISLFRCLPPSQRAITQGHPEPATFDDDERLEEQIVFDG 189

Query: 197 HSRLSFSLHSSPPSFYPPIQVSGCASAPGYLYSNHQAQPPPHQQFSCRSPSSLLQSPSLQ 256
            + +   L      +   + +         +Y    A P   Q  SC +   L+   ++ 
Sbjct: 190 ETEV---LKKENRDYVRMMDMK--------VYPEVSAVP---QSKSCENFDVLVHLKAVT 249

Query: 257 TPRISGNPRNRARAYLSVKLAHQRA-TDLVLVV----CSNGPHLRLLKQAMALVVSSLRS 316
             +IS                ++RA  DLV V+       G  L LLK+AM  V+ +L S
Sbjct: 250 GDQIS---------------QYRRAPIDLVTVLDISGSMGGTKLALLKRAMGFVIQNLGS 309

Query: 317 IDRLAIVTYSSAATRVFSLRRMTSYGKRAALQVIDRLFYMGQADAVEGLKKAIKILEDST 376
            DRL+++ +SS A R+F L RM+  G++ ALQ ++ L   G  + V+GL+K  K++ED  
Sbjct: 310 SDRLSVIAFSSTARRLFPLTRMSDAGRQLALQAVNSLVANGGTNIVDGLRKGAKVMEDRL 369

Query: 377 HKNPNSSILHLSDTRTQPYHLIDFESPAIPIHWFH-VGMGLSPSTGCVMQEFEEFLVTKV 436
            +N  +SI+ LSD R    +  +   P+        +G  LS +   +  E E       
Sbjct: 370 ERNSVASIILLSDGRDT--YTTNHPDPSYKDALAQCIGGLLSVAVQELRVEIEGVSPNVR 429

Query: 437 LRGIIRDIQLRIGEESNRSSIIRIGELRGDEEKRIIIDSQLS-DSEGH---VRVRYSYVD 479
           L  I       +      S ++ +G+L  DEE+  ++   +  + +GH   +++R  Y++
Sbjct: 430 LSSIKAGSYSSLVTGDGHSGLVDLGDLYADEERDFLVSINIPVEEDGHTPLLKLRCLYIN 453