MC01g1066 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g1066
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	protein NEN4-like
Location	MC01: 16098671 .. 16099889 (-)
RNA-Seq Expression	MC01g1066
Synteny	MC01g1066

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCTTCAAGTTCGTGCAAGGATGGTGCAGCCGAGATCGTCTTCTTCGACTTGGAAACCACGGTGCCGAATAGAGTGGGGCAGCGGTTTCGAGTATTGGAATTTGGTGCCATTGTTGTCTGCCCCCTTAAGCTCGTGGAGCTTGAGAGCTTCACCACCCTCATTAGACCAACGGATTTGTCAGCCGTCACTTCGAGGTCCAGCCGACGTGACGGGATAACAAGAGAAACCGTTGCAACTGCACCATTGTTTGAGGAAGTTGCGGACAAGATATTCAGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCAAAGATTTGACTGTGTTCGTATCAGGGAGGCGTTTGCAGAGATTGGCAGGCCTGCTCCTACGCCAGTCGGGATGGTTGATTCTTTAGGAGTCTTAACTGCAAAGTTTGGCAAGAGAGCTGGAAATATGAAGGTAAGAAAACAACATTAGAATTAGAATCATGAAAAAGAAAATCATAACATCCAGAATAGAATCCTCATATTTTGAACTGTTTCATGTTTAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGTACATAATATTAGATCAACTCCCTCCCTCTGCCTTTTTGTTATAATATCACTTTGGTTCACAAAAGTTCCTTTTGCAGGAGCCTCGACGATGTTCGTATGAATCTAGAGGTCCTCAAGCATTGTGCTACTGTTCTGTTTCTGGTATGTGCACAAAAGTTTCGACCATTTATGCAGCTCTTCTTGCATACCTGAGTTATGTAAATGGAATTTCTCTTTGTGGGTCACCTCTGATTTCGCTAATTTCTCTACCAGGAATCGACCCTCCCAAGCATATTACACGGAAAGTGGAAAACTTCATCCACCACAACAACTCGAAACAGAGCCAACGGGAAGTTGCATTGCAGAGAAGAAAGTAGTCGAAAATCTCCTCCAACGTCACCAGGATATCAACTGAGATCAGTTCCTTATGCAAGAGAGAGATTGGGAATTGGAAAGGTTCAGTTACAGGTCTTTCTTTAAATGTCAATGATATTTCATATATACATATATGGTGAAATTTTTTTCCAATCAACTGATGAAAACCTGCTTATTTGAAACAGATGACAGAAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGAAAACAGAATTTTGACATCTTACTCGAACATTCTCGTTCGCTGCTACGA

mRNA sequence

ATGGAGGCTTCAAGTTCGTGCAAGGATGGTGCAGCCGAGATCGTCTTCTTCGACTTGGAAACCACGGTGCCGAATAGAGTGGGGCAGCGGTTTCGAGTATTGGAATTTGGTGCCATTGTTGTCTGCCCCCTTAAGCTCGTGGAGCTTGAGAGCTTCACCACCCTCATTAGACCAACGGATTTGTCAGCCGTCACTTCGAGGTCCAGCCGACGTGACGGGATAACAAGAGAAACCGTTGCAACTGCACCATTGTTTGAGGAAGTTGCGGACAAGATATTCAGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCAAAGATTTGACTGTGTTCGTATCAGGGAGGCGTTTGCAGAGATTGGCAGGCCTGCTCCTACGCCAGTCGGGATGGTTGATTCTTTAGGAGTCTTAACTGCAAAGTTTGGCAAGAGAGCTGGAAATATGAAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGAGCCTCGACGATGTTCGTATGAATCTAGAGGTCCTCAAGCATTGTGCTACTGTTCTGTTTCTGGAATCGACCCTCCCAAGCATATTACACGGAAAGTGGAAAACTTCATCCACCACAACAACTCGAAACAGAGCCAACGGGAAGTTGCATTGCAGAGAAGAAAGTAGTCGAAAATCTCCTCCAACGTCACCAGGATATCAACTGAGATCAGTTCCTTATGCAAGAGAGAGATTGGGAATTGGAAAGATGACAGAAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGAAAACAGAATTTTGACATCTTACTCGAACATTCTCGTTCGCTGCTACGA

Coding sequence (CDS)

ATGGAGGCTTCAAGTTCGTGCAAGGATGGTGCAGCCGAGATCGTCTTCTTCGACTTGGAAACCACGGTGCCGAATAGAGTGGGGCAGCGGTTTCGAGTATTGGAATTTGGTGCCATTGTTGTCTGCCCCCTTAAGCTCGTGGAGCTTGAGAGCTTCACCACCCTCATTAGACCAACGGATTTGTCAGCCGTCACTTCGAGGTCCAGCCGACGTGACGGGATAACAAGAGAAACCGTTGCAACTGCACCATTGTTTGAGGAAGTTGCGGACAAGATATTCAGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCAAAGATTTGACTGTGTTCGTATCAGGGAGGCGTTTGCAGAGATTGGCAGGCCTGCTCCTACGCCAGTCGGGATGGTTGATTCTTTAGGAGTCTTAACTGCAAAGTTTGGCAAGAGAGCTGGAAATATGAAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGAGCCTCGACGATGTTCGTATGAATCTAGAGGTCCTCAAGCATTGTGCTACTGTTCTGTTTCTGGAATCGACCCTCCCAAGCATATTACACGGAAAGTGGAAAACTTCATCCACCACAACAACTCGAAACAGAGCCAACGGGAAGTTGCATTGCAGAGAAGAAAGTAGTCGAAAATCTCCTCCAACGTCACCAGGATATCAACTGAGATCAGTTCCTTATGCAAGAGAGAGATTGGGAATTGGAAAGATGACAGAAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGAAAACAGAATTTTGACATCTTACTCGAACATTCTCGTTCGCTGCTACGA

Protein sequence

MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYARERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR

Homology

[Close]

BLAST of MC01g1066 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.3e-90
Identity = 176/268 (65.67%), Postives = 203/268 (75.75%), Query Frame = 0


Query: 13  EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRRD 72
           EIVFFDLET VPN+ GQ F +LEFGAI+VCP KL ELESFTTLI+P DLS V+ RSSR D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 73  GITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGMV 132
           GITR  V  AP FE+VA+KI  +LNGRIWAGHNI+RFDCVRI+EAFAEIG+ AP P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 133 DSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 192
           DSLG+L+ KFGKRAGNMKMASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 193 SILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQL--RSVPYARERLGIGKMT 252
           + L GKW TSS   TR+R N                   YQ+  R++PY++  L   KMT
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

Query: 253 EKVKNVLCRAQGKQNFDILLEHSRSLLR 279
           + VKN+L +AQG Q    L+ HS SLLR
Sbjct: 249 QNVKNLLSKAQGNQTLQSLINHSHSLLR 255

[Close]

BLAST of MC01g1066 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-70
Identity = 145/271 (53.51%), Postives = 180/271 (66.42%), Query Frame = 0


Query: 3   ASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLS 62
           ASS   D  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 63  AVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIG 122
            +++ + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 123 RPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 182
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 183 TVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCR-----EESSRKSPPTSPGYQLRSV 242
           TVLFLES++P IL      S   + R R+NGKL          SS  SP T P       
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDA 241

Query: 243 PYARERLGIGKMTEKVKNVLCRAQGKQNFDI 269
              +    +  +TE      C      ++DI
Sbjct: 242 TIVKNHPIVSLLTE------CSESDASSYDI 266

[Close]

BLAST of MC01g1066 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.0e-67
Identity = 129/199 (64.82%), Postives = 156/199 (78.39%), Query Frame = 0


Query: 12  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRR 71
           +EI FFD+ETTVP R GQRF +LEFG+I+VCP KL EL S+TTL++P DLS ++S S R 
Sbjct: 7   SEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLSVRC 66

Query: 72  DGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 131
           +GI R+ V  APLF ++AD ++ IL+GRIWAGHNI RFDC RIREAFAEIGR  P P G 
Sbjct: 67  NGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKGA 126

Query: 132 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 191
           +DSLG+LT KFG+RAG+MKMA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 127 IDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLESSL 186

Query: 192 PSILHGKWKTSSTTTTRNR 211
           P        +  T ++R R
Sbjct: 187 PYAHVDNSVSPETISSRRR 204

[Close]

BLAST of MC01g1066 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.9e-65
Identity = 127/219 (57.99%), Postives = 165/219 (75.34%), Query Frame = 0


Query: 12  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRR 71
           +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+ +T RS + 
Sbjct: 14  SEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRSVKC 73

Query: 72  DGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 131
           +GI RE V +A  F ++AD ++ IL+GRIWAGHNI +FDC RIREAFAEIGR  P P G 
Sbjct: 74  NGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKGT 133

Query: 132 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 191
           +DSL +LT +FG+RAG+MKMA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLES+L
Sbjct: 134 IDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLESSL 193

Query: 192 PSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSP 231
           P  L     T++T  T +R       R  + +KSP  SP
Sbjct: 194 PDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of MC01g1066 vs. NCBI nr
Match: XP_022133010.1 (protein NEN4 isoform X2 [Momordica charantia])

HSP 1 Score: 544 bits (1402), Expect = 5.01e-195
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278
           RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR
Sbjct: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278

[Close]

BLAST of MC01g1066 vs. NCBI nr
Match: XP_022133009.1 (protein NEN4 isoform X1 [Momordica charantia])

HSP 1 Score: 538 bits (1387), Expect = 1.12e-192
Identity = 278/282 (98.58%), Postives = 278/282 (98.58%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIGK----MTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278
           RERLGIGK    MTEKVKNVLCRAQGKQNFDILLEHSRSLLR
Sbjct: 241 RERLGIGKVQLQMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 282

[Close]

BLAST of MC01g1066 vs. NCBI nr
Match: XP_023518830.1 (protein NEN4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494 bits (1272), Expect = 3.26e-175
Identity = 258/278 (92.81%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSS K+GA EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSFKEGA-EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAV +RSSRRDGITRE VATAPLFEEV+DKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVSDKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLL 277
            RERLGI KMT KVKNV+CRAQGKQ F+ILL+HSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MC01g1066 vs. NCBI nr
Match: XP_022962945.1 (protein NEN4-like [Cucurbita moschata])

HSP 1 Score: 494 bits (1272), Expect = 3.26e-175
Identity = 258/278 (92.81%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSS K+GA EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRPTD
Sbjct: 1   MEASSSFKEGA-EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAV +RSSRRDGITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLL 277
            RERLGI KMT KVKNV+CRAQGKQ F+ILL+HSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MC01g1066 vs. NCBI nr
Match: KAG7026830.1 (Protein NEN4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 493 bits (1268), Expect = 1.33e-174
Identity = 257/278 (92.45%), Postives = 264/278 (94.96%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSS K+GA EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRPTD
Sbjct: 1   MEASSSFKEGA-EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAV +RSSRRDGITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAF E
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFVE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILEGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLL 277
            RERLGI KMT KVKNV+CRAQGKQ F+ILL+HSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MC01g1066 vs. ExPASy TrEMBL
Match: A0A6J1BU43 (protein NEN4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1)

HSP 1 Score: 544 bits (1402), Expect = 2.42e-195
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278
           RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR
Sbjct: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278

[Close]

BLAST of MC01g1066 vs. ExPASy TrEMBL
Match: A0A6J1BUQ8 (protein NEN4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1)

HSP 1 Score: 538 bits (1387), Expect = 5.44e-193
Identity = 278/282 (98.58%), Postives = 278/282 (98.58%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 241 RERLGIGK----MTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278
           RERLGIGK    MTEKVKNVLCRAQGKQNFDILLEHSRSLLR
Sbjct: 241 RERLGIGKVQLQMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 282

[Close]

BLAST of MC01g1066 vs. ExPASy TrEMBL
Match: A0A6J1HGK3 (protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111463299 PE=4 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 1.58e-175
Identity = 258/278 (92.81%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSS K+GA EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRPTD
Sbjct: 1   MEASSSFKEGA-EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAV +RSSRRDGITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 241 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLL 277
            RERLGI KMT KVKNV+CRAQGKQ F+ILL+HSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MC01g1066 vs. ExPASy TrEMBL
Match: A0A6J1KYA7 (protein NEN4 OS=Cucurbita maxima OX=3661 GN=LOC111497460 PE=4 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 3.05e-173
Identity = 255/278 (91.73%), Postives = 262/278 (94.24%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEASSS K+GA EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSFKEGA-EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAV +RSSR DGITRE VATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRHDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IG+PAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGKPAPNPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQ RSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQARSVPYA 240

Query: 241 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLL 277
            RERLGI KMT KVKNV+C AQGKQ F+ILL+HSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCSAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MC01g1066 vs. ExPASy TrEMBL
Match: A0A5D3CL58 (Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002650 PE=4 SV=1)

HSP 1 Score: 484 bits (1246), Expect = 1.67e-171
Identity = 248/278 (89.21%), Postives = 260/278 (93.53%), Query Frame = 0


Query: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60
           MEAS S K+GA EIVFFDLETTVP RVGQRFRVLEFGAIVVCP+KLVELESF TLI+PTD
Sbjct: 5   MEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLIKPTD 64

Query: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120
           LSAV  RSSR DGITRE VATAPLFE+VADKIFSILNGRIWAGHNI+RFDCVRI+EAFAE
Sbjct: 65  LSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAE 124

Query: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180
           IGRPAPTPVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 125 IGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184

Query: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240
           CATVLFLESTLPSIL  KWKTS T TTRNRANGKLHCREESSRKSPP+SPGYQLRSVPY+
Sbjct: 185 CATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRSVPYS 244

Query: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278
           RERLGIGKMT KVKNVL  AQGKQ+ ++LL+HSRSLLR
Sbjct: 245 RERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 282

[Close]

BLAST of MC01g1066 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 9.1e-92
Identity = 176/268 (65.67%), Postives = 203/268 (75.75%), Query Frame = 0


Query: 13  EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRRD 72
           EIVFFDLET VPN+ GQ F +LEFGAI+VCP KL ELESFTTLI+P DLS V+ RSSR D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 73  GITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGMV 132
           GITR  V  AP FE+VA+KI  +LNGRIWAGHNI+RFDCVRI+EAFAEIG+ AP P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 133 DSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 192
           DSLG+L+ KFGKRAGNMKMASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 193 SILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQL--RSVPYARERLGIGKMT 252
           + L GKW TSS   TR+R N                   YQ+  R++PY++  L   KMT
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

Query: 253 EKVKNVLCRAQGKQNFDILLEHSRSLLR 279
           + VKN+L +AQG Q    L+ HS SLLR
Sbjct: 249 QNVKNLLSKAQGNQTLQSLINHSHSLLR 255

[Close]

BLAST of MC01g1066 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 1.8e-71
Identity = 145/271 (53.51%), Postives = 180/271 (66.42%), Query Frame = 0


Query: 3   ASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLS 62
           ASS   D  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 63  AVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIG 122
            +++ + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 123 RPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 182
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 183 TVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCR-----EESSRKSPPTSPGYQLRSV 242
           TVLFLES++P IL      S   + R R+NGKL          SS  SP T P       
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDA 241

Query: 243 PYARERLGIGKMTEKVKNVLCRAQGKQNFDI 269
              +    +  +TE      C      ++DI
Sbjct: 242 TIVKNHPIVSLLTE------CSESDASSYDI 266

[Close]

BLAST of MC01g1066 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.4e-68
Identity = 129/199 (64.82%), Postives = 156/199 (78.39%), Query Frame = 0


Query: 12  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRR 71
           +EI FFD+ETTVP R GQRF +LEFG+I+VCP KL EL S+TTL++P DLS ++S S R 
Sbjct: 7   SEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLSVRC 66

Query: 72  DGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 131
           +GI R+ V  APLF ++AD ++ IL+GRIWAGHNI RFDC RIREAFAEIGR  P P G 
Sbjct: 67  NGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKGA 126

Query: 132 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 191
           +DSLG+LT KFG+RAG+MKMA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 127 IDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLESSL 186

Query: 192 PSILHGKWKTSSTTTTRNR 211
           P        +  T ++R R
Sbjct: 187 PYAHVDNSVSPETISSRRR 204

[Close]

BLAST of MC01g1066 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.3e-66
Identity = 127/219 (57.99%), Postives = 165/219 (75.34%), Query Frame = 0


Query: 12  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVTSRSSRR 71
           +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+ +T RS + 
Sbjct: 14  SEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRSVKC 73

Query: 72  DGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 131
           +GI RE V +A  F ++AD ++ IL+GRIWAGHNI +FDC RIREAFAEIGR  P P G 
Sbjct: 74  NGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKGT 133

Query: 132 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 191
           +DSL +LT +FG+RAG+MKMA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLES+L
Sbjct: 134 IDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLESSL 193

Query: 192 PSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSP 231
           P  L     T++T  T +R       R  + +KSP  SP
Sbjct: 194 PDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of MC01g1066 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 240.0 bits (611), Expect = 2.3e-63
Identity = 136/271 (50.18%), Postives = 169/271 (62.36%), Query Frame = 0


Query: 3   ASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLS 62
           ASS   D  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 63  AVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAEIG 122
            +++ + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 123 RPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 182
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLE      
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE------ 181

Query: 183 TVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCR-----EESSRKSPPTSPGYQLRSV 242
                ES++P IL      S   + R R+NGKL          SS  SP T P       
Sbjct: 182 -----ESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDA 241

Query: 243 PYARERLGIGKMTEKVKNVLCRAQGKQNFDI 269
              +    +  +TE      C      ++DI
Sbjct: 242 TIVKNHPIVSLLTE------CSESDASSYDI 255

The following BLAST results are available for this feature:

BLAST of MC01g1066 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJ23	1.3e-90	65.67	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]
Q9CA74	2.5e-70	53.51	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
Q9FLR0	2.0e-67	64.82	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q0V842	1.9e-65	57.99	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]

BLAST of MC01g1066 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133010.1	5.01e-195	100.00	protein NEN4 isoform X2 [Momordica charantia]	[more]
XP_022133009.1	1.12e-192	98.58	protein NEN4 isoform X1 [Momordica charantia]	[more]
XP_023518830.1	3.26e-175	92.81	protein NEN4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022962945.1	3.26e-175	92.81	protein NEN4-like [Cucurbita moschata]	[more]
KAG7026830.1	1.33e-174	92.45	Protein NEN4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MC01g1066 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BU43	2.42e-195	100.00	protein NEN4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1	[more]
A0A6J1BUQ8	5.44e-193	98.58	protein NEN4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1	[more]
A0A6J1HGK3	1.58e-175	92.81	protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111463299 PE=4 SV=1	[more]
A0A6J1KYA7	3.05e-173	91.73	protein NEN4 OS=Cucurbita maxima OX=3661 GN=LOC111497460 PE=4 SV=1	[more]
A0A5D3CL58	1.67e-171	89.21	Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of MC01g1066 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39810.1	9.1e-92	65.67	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.1	1.8e-71	53.51	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G07710.1	1.4e-68	64.82	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61390.1	1.3e-66	57.99	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.2	2.3e-63	50.18	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013520	Exonuclease, RNase T/DNA polymerase III	SMART	SM00479	exoiiiendus	coord: 13..187 e-value: 8.5E-32 score: 121.6
IPR013520	Exonuclease, RNase T/DNA polymerase III	PFAM	PF00929	RNase_T	coord: 15..178 e-value: 2.9E-29 score: 102.7
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 8..185 e-value: 3.8E-31 score: 110.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 199..234
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 5..278
None	No IPR available	PANTHER	PTHR30231:SF26	PROTEIN NEN4	coord: 5..278
None	No IPR available	CDD	cd06127	DEDDh	coord: 15..179 e-value: 3.58132E-26 score: 98.912
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 14..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g1066.1	MC01g1066.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding