MC01g0750 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0750
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	MC01: 13669018 .. 13670307 (+)
RNA-Seq Expression	MC01g0750
Synteny	MC01g0750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCGTTTCTGCGGAAGGCACACTCGGAGTGAACTGGGGAACGGTAGCGACGAACCCATTGGACGGGAGCATCGTGGTGCAGTTGCTGAAGGATAATGGAATAAAAAGAGTGAAGCTTTTCGATTTGGATTCCGCGGCTAATCTATCTGTCTTGGCTGGTACCGGAATTAAGACCATCGTCGGCATCCGTAATGAGCTCCTTGAGACATTTGCAACCGATTACAACCTTGTCAAAGATTGGGTTAAGCAAAATATTTCCACTTACATGGGTGAAGGGGGTGTTGATATAATATACATCGCCGTAGGAAACGAACCATTCTTGGGGAATTACCAAGGGAAGTACGTCAATACCACATTCCCGGCCATGCAAAATGTACAAAAGGCTCTGGAAGCTGCCGGACATAGCGACAAAATCAAAGTCACAACGCCATTGAACGGCGACATTTACGAAACCCCAACTAACCTCCCTTCCGACGGCAGGTTCCGAAGCAACATTTACGAGCCCGTGAAGCAAATAGTCCAATTCCTCGACCAACACAAATCTCCATTCATGGTCAACATCTACCCTTTCATCAACCTCTATCAAAACCCTGGCTTTCCATTCAATTACGCCTTCTTCGACAACGACGGCAAGCTCGACAAGAACAGCTTCGTCAACGACAAGGGCACCATTTACTACAACATGTTGGACGCACATTACGACACGCTGACGTGGTCGCTCAAGGACGTGGGCGTAACCGACATGAAAATCATCATCGGAGAAGTGGGGTGGCCGACGGACGGGAACATATATGCGAACACGACGGTAGCGAAGAGATTCTACAGCGGGCTGTTAAAGAGAATGGCGTCCAACAAAGGGACTCCAATGAGGCCGAATGAGGATTATGAGGTGTATATGTTTGGCCTTTTGGACGAGAACAACAAGAGCGTTGACCCGGGGAATTTCGAGCGCCATTGGGGGATTTTCCGGTACGACGGGACCCCCAAGTTCGCGATGGACCTTTCCTTGCAGGGGGTGGAGAAGATGCCGGTGGCGGCGAAAGGGGTTCGGTATATGAAGGATCAGTGGTGTGTTATTGCGGATAATGTGACTGATTTGGGAGCGAGTAATATACAAAAAGATGTGTCGTGGGCGTGCCAGAATTCGGATTGCACGAGCATGGGTTATGGGTGCTCCTGCAACAACTTGGGTGCGCGAGGGAATGTTTCGTATGTTTTCAACATGTATTATCAGACGCAGAATCAGAATCCGGAGGCGTGCGATTTTGGAGGGGCGGCCAAGATTGTGACC

mRNA sequence

GGCGTTTCTGCGGAAGGCACACTCGGAGTGAACTGGGGAACGGTAGCGACGAACCCATTGGACGGGAGCATCGTGGTGCAGTTGCTGAAGGATAATGGAATAAAAAGAGTGAAGCTTTTCGATTTGGATTCCGCGGCTAATCTATCTGTCTTGGCTGGTACCGGAATTAAGACCATCGTCGGCATCCGTAATGAGCTCCTTGAGACATTTGCAACCGATTACAACCTTGTCAAAGATTGGGTTAAGCAAAATATTTCCACTTACATGGGTGAAGGGGGTGTTGATATAATATACATCGCCGTAGGAAACGAACCATTCTTGGGGAATTACCAAGGGAAGTACGTCAATACCACATTCCCGGCCATGCAAAATGTACAAAAGGCTCTGGAAGCTGCCGGACATAGCGACAAAATCAAAGTCACAACGCCATTGAACGGCGACATTTACGAAACCCCAACTAACCTCCCTTCCGACGGCAGGTTCCGAAGCAACATTTACGAGCCCGTGAAGCAAATAGTCCAATTCCTCGACCAACACAAATCTCCATTCATGGTCAACATCTACCCTTTCATCAACCTCTATCAAAACCCTGGCTTTCCATTCAATTACGCCTTCTTCGACAACGACGGCAAGCTCGACAAGAACAGCTTCGTCAACGACAAGGGCACCATTTACTACAACATGTTGGACGCACATTACGACACGCTGACGTGGTCGCTCAAGGACGTGGGCGTAACCGACATGAAAATCATCATCGGAGAAGTGGGGTGGCCGACGGACGGGAACATATATGCGAACACGACGGTAGCGAAGAGATTCTACAGCGGGCTGTTAAAGAGAATGGCGTCCAACAAAGGGACTCCAATGAGGCCGAATGAGGATTATGAGGTGTATATGTTTGGCCTTTTGGACGAGAACAACAAGAGCGTTGACCCGGGGAATTTCGAGCGCCATTGGGGGATTTTCCGGTACGACGGGACCCCCAAGTTCGCGATGGACCTTTCCTTGCAGGGGGTGGAGAAGATGCCGGTGGCGGCGAAAGGGGTTCGGTATATGAAGGATCAGTGGTGTGTTATTGCGGATAATGTGACTGATTTGGGAGCGAGTAATATACAAAAAGATGTGTCGTGGGCGTGCCAGAATTCGGATTGCACGAGCATGGGTTATGGGTGCTCCTGCAACAACTTGGGTGCGCGAGGGAATGTTTCGTATGTTTTCAACATGTATTATCAGACGCAGAATCAGAATCCGGAGGCGTGCGATTTTGGAGGGGCGGCCAAGATTGTGACC

Coding sequence (CDS)

GGCGTTTCTGCGGAAGGCACACTCGGAGTGAACTGGGGAACGGTAGCGACGAACCCATTGGACGGGAGCATCGTGGTGCAGTTGCTGAAGGATAATGGAATAAAAAGAGTGAAGCTTTTCGATTTGGATTCCGCGGCTAATCTATCTGTCTTGGCTGGTACCGGAATTAAGACCATCGTCGGCATCCGTAATGAGCTCCTTGAGACATTTGCAACCGATTACAACCTTGTCAAAGATTGGGTTAAGCAAAATATTTCCACTTACATGGGTGAAGGGGGTGTTGATATAATATACATCGCCGTAGGAAACGAACCATTCTTGGGGAATTACCAAGGGAAGTACGTCAATACCACATTCCCGGCCATGCAAAATGTACAAAAGGCTCTGGAAGCTGCCGGACATAGCGACAAAATCAAAGTCACAACGCCATTGAACGGCGACATTTACGAAACCCCAACTAACCTCCCTTCCGACGGCAGGTTCCGAAGCAACATTTACGAGCCCGTGAAGCAAATAGTCCAATTCCTCGACCAACACAAATCTCCATTCATGGTCAACATCTACCCTTTCATCAACCTCTATCAAAACCCTGGCTTTCCATTCAATTACGCCTTCTTCGACAACGACGGCAAGCTCGACAAGAACAGCTTCGTCAACGACAAGGGCACCATTTACTACAACATGTTGGACGCACATTACGACACGCTGACGTGGTCGCTCAAGGACGTGGGCGTAACCGACATGAAAATCATCATCGGAGAAGTGGGGTGGCCGACGGACGGGAACATATATGCGAACACGACGGTAGCGAAGAGATTCTACAGCGGGCTGTTAAAGAGAATGGCGTCCAACAAAGGGACTCCAATGAGGCCGAATGAGGATTATGAGGTGTATATGTTTGGCCTTTTGGACGAGAACAACAAGAGCGTTGACCCGGGGAATTTCGAGCGCCATTGGGGGATTTTCCGGTACGACGGGACCCCCAAGTTCGCGATGGACCTTTCCTTGCAGGGGGTGGAGAAGATGCCGGTGGCGGCGAAAGGGGTTCGGTATATGAAGGATCAGTGGTGTGTTATTGCGGATAATGTGACTGATTTGGGAGCGAGTAATATACAAAAAGATGTGTCGTGGGCGTGCCAGAATTCGGATTGCACGAGCATGGGTTATGGGTGCTCCTGCAACAACTTGGGTGCGCGAGGGAATGTTTCGTATGTTTTCAACATGTATTATCAGACGCAGAATCAGAATCCGGAGGCGTGCGATTTTGGAGGGGCGGCCAAGATTGTGACC

Protein sequence

GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVGIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPAMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKSPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLKDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIADNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACDFGGAAKIVT

Homology

[Close]

BLAST of MC01g0750 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 4.7e-124
Identity = 218/432 (50.46%), Postives = 293/432 (67.82%), Query Frame = 0


Query: 1   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIV 60
           G+   G LGVNWGT+AT+ L    VVQ+LKDN I +VKLFD D    +  LAG+G++ +V
Sbjct: 19  GILVNG-LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADE-TTMGALAGSGLEVMV 78

Query: 61  GIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFP 120
            I N+ L+   T Y+  KDWV++N++ Y  +GGV+I ++AVGNEPFL +Y G ++N TFP
Sbjct: 79  AIPNDQLKVM-TSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFP 138

Query: 121 AMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTN--LPSDGRFRSNIYEPVKQIVQFLDQ 180
           A+ N+Q AL  AG  + +K T PLN D+Y++P +  +PS GRFR +I   + QIV FL +
Sbjct: 139 ALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGK 198

Query: 181 HKSPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTW 240
           + +P  +NIYPF++LY N  FP NYAFFD          +ND G  Y N+ DA++DTL  
Sbjct: 199 NNAPITINIYPFLSLYGNDDFPLNYAFFDGAEP------INDNGIDYTNVFDANFDTLVS 258

Query: 241 SLKDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVY 300
           SLK VG  DM II+GEVGWPT+G+ +AN   A RFY+GLL R+ +NKGTP+RP    EVY
Sbjct: 259 SLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTY-IEVY 318

Query: 301 MFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCV 360
           +FGLLDE+ KS+ PG FERHWGIF++DG PKF +DLS QG  K  + A+ V Y+ ++WC 
Sbjct: 319 LFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCT 378

Query: 361 IADNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPE 420
                 DL  + +  ++ +AC  SDCT++GYG SCN L A GN SY FNM++Q +NQ+  
Sbjct: 379 FNPEAKDL--TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDES 438

Query: 421 ACDFGGAAKIVT 431
           AC F G A I T
Sbjct: 439 ACYFQGLATITT 438

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BLAST of MC01g0750 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.9e-118
Identity = 210/429 (48.95%), Postives = 284/429 (66.20%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           +  EG +GVNWG+ A +PL  + VV+LL++NGI++VKLF+ DSA  L  L+ TGI+ +VG
Sbjct: 22  IEVEG-IGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAI-LKALSRTGIQVMVG 81

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           I N+LL   A      + WV QN+S ++   GVDI Y+AVGNEPFL  + G +   T PA
Sbjct: 82  IPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTFEGITLPA 141

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           +QN+Q A+  AG + ++KVT PLN D+Y++ +NLPSDG FR  I + +  IV+FL  + +
Sbjct: 142 LQNIQSAIIKAGLATQVKVTVPLNADVYQSASNLPSDGDFRPEIRDLMLNIVKFLSDNGA 201

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PF +NIYPFI+LY +P FP  +AFFD  G     + +ND G IY N+LDA+YDTL WSL+
Sbjct: 202 PFTINIYPFISLYNDPNFPVEFAFFDGTG-----TPINDNGRIYDNVLDANYDTLVWSLQ 261

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
             G  ++ II+GEVGWPTDG+  AN   A+R+  G + R  +NKGTPMRP    + Y+FG
Sbjct: 262 KNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGA-MDAYLFG 321

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
           L+DE+ KS+ PGNFERHWGIF  DG PK+ + L   G     + AK V Y+  +WC++A 
Sbjct: 322 LIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSL---GSGNGLIPAKDVHYLAKKWCILAP 381

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           N  +L    +   VS+AC ++DCTS+GYG SC NL    NVSY FN YYQ  NQ   AC 
Sbjct: 382 N-ANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSACK 438

Query: 422 FGGAAKIVT 431
           F G + + T
Sbjct: 442 FPGLSIVST 438

[Close]

BLAST of MC01g0750 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 4.7e-116
Identity = 203/422 (48.10%), Postives = 283/422 (67.06%), Query Frame = 0


Query: 7   TLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVGIRNEL 66
           ++G NWGT A++PL   IVV++L++NGI++VKLFD +    L  L  +GI+ +VGI NE+
Sbjct: 21  SIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAE-YDTLRALGKSGIEVMVGIPNEM 80

Query: 67  LETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPAMQNVQ 126
           L T A+     + WV +N+ST++    V+I Y+AVGNEPFL  Y G Y++TTFPA++N+Q
Sbjct: 81  LATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSYLSTTFPALRNIQ 140

Query: 127 KALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKSPFMVN 186
            A+  AG  +++KVT PLN D+Y++ T  PS G FR+NI + +  IV+FL ++  PF VN
Sbjct: 141 IAIIKAGLQNQVKVTCPLNADVYDSSTTFPSGGDFRANIRDLMITIVKFLSENGGPFTVN 200

Query: 187 IYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLKDVGVT 246
           IYP+I+LY NP FP +YAFFD + +      +ND GT YYNM DA+YDTL  +L+  G  
Sbjct: 201 IYPYISLYTNPDFPVDYAFFDGNAQP-----LNDGGTFYYNMFDANYDTLVHALEKNGFG 260

Query: 247 DMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFGLLDEN 306
           +M IIIGE+GWPTDG+  AN   AK+F  G +  ++  KGTP RP    + Y+F L+DE+
Sbjct: 261 NMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPG-PIDAYLFSLIDED 320

Query: 307 NKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIADNVTDL 366
            KSV PG FERHWGIF +DG PK+A++L       + + AKGVRY++ +WCV+  NV  L
Sbjct: 321 AKSVQPGYFERHWGIFTFDGLPKYALNLGTTNTGAL-IQAKGVRYLERKWCVMKPNVR-L 380

Query: 367 GASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACDFGGAA 426
               +   VS+AC   DCTS+G G SC NL  + N+SY FN YYQ Q+Q   AC F   +
Sbjct: 381 DDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTACKFPNIS 433

Query: 427 KI 429
           ++
Sbjct: 441 EV 433

[Close]

BLAST of MC01g0750 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.1e-80
Identity = 163/435 (37.47%), Postives = 245/435 (56.32%), Query Frame = 0


Query: 1   GVSAEGT----LGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGI 60
           G+S  GT    +G+NWGT A++PL  S VV+LLK NGI +VKLFD D    L  L+G+ I
Sbjct: 15  GLSLTGTTVRAVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKV-LRALSGSNI 74

Query: 61  KTIVGIRNELLETFATDYNLVKDWVKQNISTYMGEGG-VDIIYIAVGNEPFLGNYQGKYV 120
              +GI+N +L++      + + WV  N++ Y   G  V I Y+AVG EPFL +Y  +Y 
Sbjct: 75  GVTIGIQNSMLKSLNASVKVAESWVHDNVTRYFNGGNRVRIEYVAVGEEPFLQSYGNQYK 134

Query: 121 NTTFPAMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQF 180
                A  N+Q AL  A  ++++KV  P + D + + +  PS G FR+++ + + +++ F
Sbjct: 135 PFVIGAAMNIQNALVKANLANEVKVVVPSSFDSFLSESGRPSSGHFRADLNKTMIELLSF 194

Query: 181 LDQHKSPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDT 240
           L +H SPF V I PF++ +QN     +++ F    K  K     D    Y N  D  YDT
Sbjct: 195 LTKHHSPFFVTISPFLSFHQNKNISLDFSLFKETAKAHK-----DGRKTYRNSFDLSYDT 254

Query: 241 LTWSLKDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDY 300
           L  +L  +G +++ I++ ++GWPTDG   A +  A+ F+ GL+  +   K T   P    
Sbjct: 255 LVSALFTIGFSEVDIVVSKIGWPTDGAENATSLTAEAFFKGLIVHL--EKKTASLPRPPV 314

Query: 301 EVYMFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQ 360
           E Y+  LLDE+ +++  GNFERHWG+F +DG  K+    +     K  V A+ V+Y+  +
Sbjct: 315 ETYIESLLDEDQRNLSAGNFERHWGVFTFDGQAKYNFSFN----HKNQVNAQNVQYLPPK 374

Query: 361 WCVIADNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQ 420
           WCV+ +N  DL  SN       AC  +DCTS+  G SC+ +   GNVSY FN  YQ  + 
Sbjct: 375 WCVVNNN-KDL--SNASARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDH 434

Query: 421 NPEACDFGGAAKIVT 431
           + E+C+FGG   I T
Sbjct: 435 SAESCNFGGLGLITT 434

[Close]

BLAST of MC01g0750 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.2e-48
Identity = 132/434 (30.41%), Postives = 222/434 (51.15%), Query Frame = 0


Query: 6   GTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVGIRNE 65
           G +GV +G  A +    S VVQL++ + IK V+++D +S   L     T I+ ++G+ N 
Sbjct: 24  GKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQV-LKAFGNTSIELMIGVPNS 83

Query: 66  LLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTT---FPAM 125
            L  F+   + V  W+K ++  Y       I YI VG E    +    ++N +    PAM
Sbjct: 84  DLNAFSQSQSNVDTWLKNSVLPYYPT--TKITYITVGAE----STDDPHINASSFVVPAM 143

Query: 126 QNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKSP 185
           QNV  AL   G S +IKV+T L+  I  + +  PS G F S+    ++ +++FL ++KSP
Sbjct: 144 QNVLTALRKVGLSRRIKVSTTLSLGIL-SRSFPPSAGAFNSSYAYFLRPMLEFLAENKSP 203

Query: 186 FMVNIYPFINLYQNP-GFPFNYAFFDNDGK-LDKNSFVNDKGTIYYNMLDAHYDTLTWSL 245
           FM+++YP+     +P     +Y  F++  + +D N+     G +Y NM DA  D L ++L
Sbjct: 204 FMIDLYPYYAYRDSPNNVSLDYVLFESSSEVIDPNT-----GLLYKNMFDAQVDALYYAL 263

Query: 246 KDVGVTDMKIIIGEVGWPTDGN----IYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYE 305
             +    +KI++ E GWPT G+      A++  A+ + S +++ + +N+GTP +P E   
Sbjct: 264 TALNFRTIKIMVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMN 323

Query: 306 VYMFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLS--LQGVEKMPVAAKGVRYMKD 365
           VY+F L +EN K+    + ER+WG+F  D T  + +D +    G      +        +
Sbjct: 324 VYIFSLFNENRKA--GLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNS-SGTNSSGSSN 383

Query: 366 QWCVIADNVTDLGASNIQKDVSWAC--QNSDCTSMGYGCSCNNLGAR-GNVSYVFNMYYQ 425
            WC+ +   ++    +++  + WAC   N DCT++     C        + S+VFN Y+Q
Sbjct: 384 SWCIASSKASE---RDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQ 438

[Close]

BLAST of MC01g0750 vs. NCBI nr
Match: XP_022133125.1 (glucan endo-1,3-beta-glucosidase 8-like, partial [Momordica charantia])

HSP 1 Score: 776 bits (2003), Expect = 1.92e-280
Identity = 371/430 (86.28%), Postives = 404/430 (93.95%), Query Frame = 0


Query: 1   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIV 60
           GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDS ANLS LAGTGIKTIV
Sbjct: 3   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSVANLSALAGTGIKTIV 62

Query: 61  GIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFP 120
           G+ N LL+TFATDYNLVKDWVKQNIS YMG+GGVDIIYIAVGNEPFLG Y+G Y NTTFP
Sbjct: 63  GVPNYLLQTFATDYNLVKDWVKQNISAYMGDGGVDIIYIAVGNEPFLGAYKGMYTNTTFP 122

Query: 121 AMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHK 180
           AMQNVQKALEAAGHSDKIKVTTPLNGD+Y++ +NLPSDGRFR++IY+P+KQIVQFLD++K
Sbjct: 123 AMQNVQKALEAAGHSDKIKVTTPLNGDVYQSFSNLPSDGRFRTDIYDPMKQIVQFLDKNK 182

Query: 181 SPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSL 240
           SPFM+NIYPFINLYQNPGFPFNYAFFDNDGK+ K+S ++DKGTIYYN+LDAHYDTLT+SL
Sbjct: 183 SPFMINIYPFINLYQNPGFPFNYAFFDNDGKV-KDSVIDDKGTIYYNVLDAHYDTLTYSL 242

Query: 241 KDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMF 300
           K++GV DMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRP E+YEVYMF
Sbjct: 243 KEMGVNDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPKENYEVYMF 302

Query: 301 GLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIA 360
            LLDEN KS+DPGNFERHWGIFRYDGTPKFAMD S  G +KMPV AKGV YMK+QWCVIA
Sbjct: 303 SLLDENIKSIDPGNFERHWGIFRYDGTPKFAMDFSGNGADKMPVGAKGVEYMKNQWCVIA 362

Query: 361 DNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEAC 420
           DNVT+LGAS IQKDVSWAC++S+CTS+GYGCSCNNLG RGNVSYVFNMYYQ Q+QNP AC
Sbjct: 363 DNVTELGAS-IQKDVSWACEHSECTSLGYGCSCNNLGVRGNVSYVFNMYYQMQDQNPAAC 422

Query: 421 DFGGAAKIVT 430
            FGGAAKIVT
Sbjct: 423 GFGGAAKIVT 430

[Close]

BLAST of MC01g0750 vs. NCBI nr
Match: XP_022132538.1 (glucan endo-1,3-beta-glucosidase 5-like isoform X1 [Momordica charantia])

HSP 1 Score: 719 bits (1856), Expect = 8.59e-258
Identity = 337/429 (78.55%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V AEG +GVNWGT+A++PL+ S+VV++LKDNGIK+VKLFDLDS A+LS L+GTGI+TIVG
Sbjct: 24  VGAEGEIGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVG 83

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           + N  L+ FAT+Y+ VKDWVKQNIS Y+ EGGVDI YIAVGNEPFL  Y+G YVN TFPA
Sbjct: 84  VPNYRLQKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPA 143

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL+AAGH DK+KVTTPLNGD+Y++ +NLPSDG+FR++IY+ +KQIV+FLD+HKS
Sbjct: 144 MQNVQKALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKS 203

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPFINLYQNPGFPFNYAF DNDGK+ K+S V+DKGTIYYN+LDAHYDTLTWSLK
Sbjct: 204 PFMVNIYPFINLYQNPGFPFNYAFLDNDGKV-KDSVVDDKGTIYYNVLDAHYDTLTWSLK 263

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
           D+GV DMKIIIGEVGWPTDGNIYAN T+AKRFY+GL KR+ASNKGTPMRPNE YE+Y+F 
Sbjct: 264 DMGVNDMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFS 323

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
            LDE+ KSVDPGNFERHWGIFRYDGTPKFAMD S +GVEKMPVAAKGV YM+ +WCV+AD
Sbjct: 324 FLDEDVKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVAD 383

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           NVTDLG  +IQKDVSWAC++SDCTS+GYGCSCN L ARGNVSYVFNMYYQ Q+QNPEAC 
Sbjct: 384 NVTDLGG-DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACG 443

Query: 422 FGGAAKIVT 430
           FGGAA+IVT
Sbjct: 444 FGGAAEIVT 450

[Close]

BLAST of MC01g0750 vs. NCBI nr
Match: XP_022132539.1 (glucan endo-1,3-beta-glucosidase 5-like isoform X2 [Momordica charantia])

HSP 1 Score: 642 bits (1657), Expect = 6.34e-229
Identity = 301/387 (77.78%), Postives = 351/387 (90.70%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V AEG +GVNWGT+A++PL+ S+VV++LKDNGIK+VKLFDLDS A+LS L+GTGI+TIVG
Sbjct: 24  VGAEGEIGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVG 83

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           + N  L+ FAT+Y+ VKDWVKQNIS Y+ EGGVDI YIAVGNEPFL  Y+G YVN TFPA
Sbjct: 84  VPNYRLQKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPA 143

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL+AAGH DK+KVTTPLNGD+Y++ +NLPSDG+FR++IY+ +KQIV+FLD+HKS
Sbjct: 144 MQNVQKALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKS 203

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPFINLYQNPGFPFNYAF DNDGK+ K+S V+DKGTIYYN+LDAHYDTLTWSLK
Sbjct: 204 PFMVNIYPFINLYQNPGFPFNYAFLDNDGKV-KDSVVDDKGTIYYNVLDAHYDTLTWSLK 263

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
           D+GV DMKIIIGEVGWPTDGNIYAN T+AKRFY+GL KR+ASNKGTPMRPNE YE+Y+F 
Sbjct: 264 DMGVNDMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFS 323

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
            LDE+ KSVDPGNFERHWGIFRYDGTPKFAMD S +GVEKMPVAAKGV YM+ +WCV+AD
Sbjct: 324 FLDEDVKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVAD 383

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMG 388
           NVTDLG  +IQKDVSWAC++SDCTS+G
Sbjct: 384 NVTDLGG-DIQKDVSWACEHSDCTSLG 408

[Close]

BLAST of MC01g0750 vs. NCBI nr
Match: KAG7011052.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 556 bits (1432), Expect = 9.22e-194
Identity = 264/429 (61.54%), Postives = 334/429 (77.86%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V A   +GVNWGT+A++P++ SIVV LLKDNGIK+VKLFD DS   +  LAG+ I+T+V 
Sbjct: 23  VEANLDVGVNWGTIASHPINPSIVVNLLKDNGIKKVKLFDSDSWI-MGALAGSKIETVVA 82

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           I N+ LE FA DY+L KDWVK+N++TY+ +GGVDI Y+AVGNEPFL  + G Y+  TFPA
Sbjct: 83  IPNDQLEKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAVGNEPFLTGFNGSYIKLTFPA 142

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL  AGH DKIKVTTPLN D+YE+P+N PSDGRFRS+I++ +K IV FL  + +
Sbjct: 143 MQNVQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRFRSDIHDTMKNIVHFLSSNNA 202

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPF++LYQNP FP ++AF D+ GK      +NDKG  Y N+ DA+YDTL WSLK
Sbjct: 203 PFMVNIYPFLSLYQNPDFPIDFAFLDSSGKS-----INDKGKTYTNVFDANYDTLVWSLK 262

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
            +G  D+KI++GEVGWPTD N YAN  +AKRFY GL K +AS KGTPMRP+E  E+Y+F 
Sbjct: 263 KIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKGTPMRPDEKLEIYLFS 322

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
           L DE+ KSV PG FERHWGIFRYDG PKF MDL+  G +KMPVAAKGV+Y++ +WCVI +
Sbjct: 323 LFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAAKGVQYLEKKWCVIKN 382

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           ++ DL +   Q D  +AC  SDCT++ +G SCN+L ARGN S+ +NMY+Q Q+Q+ EACD
Sbjct: 383 SIEDLTSEVSQID--YACSLSDCTTLVFGSSCNDLNARGNFSFAYNMYFQMQDQSVEACD 442

Query: 422 FGGAAKIVT 430
           FGG+A+IVT
Sbjct: 443 FGGSAEIVT 443

[Close]

BLAST of MC01g0750 vs. NCBI nr
Match: XP_022927955.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata])

HSP 1 Score: 556 bits (1432), Expect = 9.22e-194
Identity = 264/429 (61.54%), Postives = 334/429 (77.86%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V A   +GVNWGT+A++P++ SIVV LLKDNGIK+VKLFD DS   +  LAG+ I+T+V 
Sbjct: 23  VEANLDVGVNWGTIASHPINPSIVVNLLKDNGIKKVKLFDSDSWI-MGALAGSKIETVVA 82

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           I N+ LE FA DY+L KDWVK+N++TY+ +GGVDI Y+AVGNEPFL  + G Y+  TFPA
Sbjct: 83  IPNDQLEKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAVGNEPFLTGFNGSYIKLTFPA 142

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL  AGH DKIKVTTPLN D+YE+P+N PSDGRFRS+I++ +K IV FL  + +
Sbjct: 143 MQNVQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRFRSDIHDTMKNIVHFLSSNNA 202

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPF++LYQNP FP ++AF D+ GK      +NDKG  Y N+ DA+YDTL WSLK
Sbjct: 203 PFMVNIYPFLSLYQNPDFPIDFAFLDSSGKS-----INDKGKTYTNVFDANYDTLVWSLK 262

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
            +G  D+KI++GEVGWPTD N YAN  +AKRFY GL K +AS KGTPMRP+E  E+Y+F 
Sbjct: 263 KIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKGTPMRPDEKLEIYLFS 322

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
           L DE+ KSV PG FERHWGIFRYDG PKF MDL+  G +KMPVAAKGV+Y++ +WCVI +
Sbjct: 323 LFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAAKGVQYLEKKWCVIKN 382

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           ++ DL +   Q D  +AC  SDCT++ +G SCN+L ARGN S+ +NMY+Q Q+Q+ EACD
Sbjct: 383 SIEDLTSEVSQID--YACSLSDCTTLVFGSSCNDLNARGNFSFAYNMYFQMQDQSVEACD 442

Query: 422 FGGAAKIVT 430
           FGG+A+IVT
Sbjct: 443 FGGSAEIVT 443

[Close]

BLAST of MC01g0750 vs. ExPASy TrEMBL
Match: A0A6J1BUF0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005798 PE=3 SV=1)

HSP 1 Score: 776 bits (2003), Expect = 9.28e-281
Identity = 371/430 (86.28%), Postives = 404/430 (93.95%), Query Frame = 0


Query: 1   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIV 60
           GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDS ANLS LAGTGIKTIV
Sbjct: 3   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSVANLSALAGTGIKTIV 62

Query: 61  GIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFP 120
           G+ N LL+TFATDYNLVKDWVKQNIS YMG+GGVDIIYIAVGNEPFLG Y+G Y NTTFP
Sbjct: 63  GVPNYLLQTFATDYNLVKDWVKQNISAYMGDGGVDIIYIAVGNEPFLGAYKGMYTNTTFP 122

Query: 121 AMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHK 180
           AMQNVQKALEAAGHSDKIKVTTPLNGD+Y++ +NLPSDGRFR++IY+P+KQIVQFLD++K
Sbjct: 123 AMQNVQKALEAAGHSDKIKVTTPLNGDVYQSFSNLPSDGRFRTDIYDPMKQIVQFLDKNK 182

Query: 181 SPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSL 240
           SPFM+NIYPFINLYQNPGFPFNYAFFDNDGK+ K+S ++DKGTIYYN+LDAHYDTLT+SL
Sbjct: 183 SPFMINIYPFINLYQNPGFPFNYAFFDNDGKV-KDSVIDDKGTIYYNVLDAHYDTLTYSL 242

Query: 241 KDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMF 300
           K++GV DMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRP E+YEVYMF
Sbjct: 243 KEMGVNDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPKENYEVYMF 302

Query: 301 GLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIA 360
            LLDEN KS+DPGNFERHWGIFRYDGTPKFAMD S  G +KMPV AKGV YMK+QWCVIA
Sbjct: 303 SLLDENIKSIDPGNFERHWGIFRYDGTPKFAMDFSGNGADKMPVGAKGVEYMKNQWCVIA 362

Query: 361 DNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEAC 420
           DNVT+LGAS IQKDVSWAC++S+CTS+GYGCSCNNLG RGNVSYVFNMYYQ Q+QNP AC
Sbjct: 363 DNVTELGAS-IQKDVSWACEHSECTSLGYGCSCNNLGVRGNVSYVFNMYYQMQDQNPAAC 422

Query: 421 DFGGAAKIVT 430
            FGGAAKIVT
Sbjct: 423 GFGGAAKIVT 430

[Close]

BLAST of MC01g0750 vs. ExPASy TrEMBL
Match: A0A6J1BWJ0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 PE=3 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 4.16e-258
Identity = 337/429 (78.55%), Postives = 389/429 (90.68%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V AEG +GVNWGT+A++PL+ S+VV++LKDNGIK+VKLFDLDS A+LS L+GTGI+TIVG
Sbjct: 24  VGAEGEIGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVG 83

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           + N  L+ FAT+Y+ VKDWVKQNIS Y+ EGGVDI YIAVGNEPFL  Y+G YVN TFPA
Sbjct: 84  VPNYRLQKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPA 143

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL+AAGH DK+KVTTPLNGD+Y++ +NLPSDG+FR++IY+ +KQIV+FLD+HKS
Sbjct: 144 MQNVQKALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKS 203

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPFINLYQNPGFPFNYAF DNDGK+ K+S V+DKGTIYYN+LDAHYDTLTWSLK
Sbjct: 204 PFMVNIYPFINLYQNPGFPFNYAFLDNDGKV-KDSVVDDKGTIYYNVLDAHYDTLTWSLK 263

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
           D+GV DMKIIIGEVGWPTDGNIYAN T+AKRFY+GL KR+ASNKGTPMRPNE YE+Y+F 
Sbjct: 264 DMGVNDMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFS 323

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
            LDE+ KSVDPGNFERHWGIFRYDGTPKFAMD S +GVEKMPVAAKGV YM+ +WCV+AD
Sbjct: 324 FLDEDVKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVAD 383

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           NVTDLG  +IQKDVSWAC++SDCTS+GYGCSCN L ARGNVSYVFNMYYQ Q+QNPEAC 
Sbjct: 384 NVTDLGG-DIQKDVSWACEHSDCTSLGYGCSCNKLDARGNVSYVFNMYYQMQSQNPEACG 443

Query: 422 FGGAAKIVT 430
           FGGAA+IVT
Sbjct: 444 FGGAAEIVT 450

[Close]

BLAST of MC01g0750 vs. ExPASy TrEMBL
Match: A0A6J1BTC0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 PE=3 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 3.07e-229
Identity = 301/387 (77.78%), Postives = 351/387 (90.70%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V AEG +GVNWGT+A++PL+ S+VV++LKDNGIK+VKLFDLDS A+LS L+GTGI+TIVG
Sbjct: 24  VGAEGEIGVNWGTIASHPLEASVVVKMLKDNGIKKVKLFDLDSVAHLSALSGTGIETIVG 83

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           + N  L+ FAT+Y+ VKDWVKQNIS Y+ EGGVDI YIAVGNEPFL  Y+G YVN TFPA
Sbjct: 84  VPNYRLQKFATNYDYVKDWVKQNISAYLHEGGVDIKYIAVGNEPFLAAYKGDYVNITFPA 143

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL+AAGH DK+KVTTPLNGD+Y++ +NLPSDG+FR++IY+ +KQIV+FLD+HKS
Sbjct: 144 MQNVQKALDAAGHGDKVKVTTPLNGDVYQSFSNLPSDGKFRADIYDSMKQIVKFLDEHKS 203

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPFINLYQNPGFPFNYAF DNDGK+ K+S V+DKGTIYYN+LDAHYDTLTWSLK
Sbjct: 204 PFMVNIYPFINLYQNPGFPFNYAFLDNDGKV-KDSVVDDKGTIYYNVLDAHYDTLTWSLK 263

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
           D+GV DMKIIIGEVGWPTDGNIYAN T+AKRFY+GL KR+ASNKGTPMRPNE YE+Y+F 
Sbjct: 264 DMGVNDMKIIIGEVGWPTDGNIYANVTLAKRFYNGLFKRVASNKGTPMRPNEKYEIYLFS 323

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
            LDE+ KSVDPGNFERHWGIFRYDGTPKFAMD S +GVEKMPVAAKGV YM+ +WCV+AD
Sbjct: 324 FLDEDVKSVDPGNFERHWGIFRYDGTPKFAMDFSGEGVEKMPVAAKGVEYMERKWCVVAD 383

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMG 388
           NVTDLG  +IQKDVSWAC++SDCTS+G
Sbjct: 384 NVTDLGG-DIQKDVSWACEHSDCTSLG 408

[Close]

BLAST of MC01g0750 vs. ExPASy TrEMBL
Match: A0A6J1EQB3 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434805 PE=3 SV=1)

HSP 1 Score: 556 bits (1432), Expect = 4.46e-194
Identity = 264/429 (61.54%), Postives = 334/429 (77.86%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V A   +GVNWGT+A++P++ SIVV LLKDNGIK+VKLFD DS   +  LAG+ I+T+V 
Sbjct: 23  VEANLDVGVNWGTIASHPINPSIVVNLLKDNGIKKVKLFDSDSWI-MGALAGSKIETVVA 82

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           I N+ LE FA DY+L KDWVK+N++TY+ +GGVDI Y+AVGNEPFL  + G Y+  TFPA
Sbjct: 83  IPNDQLEKFANDYDLAKDWVKENVTTYIYDGGVDIRYVAVGNEPFLTGFNGSYIKLTFPA 142

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQNVQKAL  AGH DKIKVTTPLN D+YE+P+N PSDGRFRS+I++ +K IV FL  + +
Sbjct: 143 MQNVQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRFRSDIHDTMKNIVHFLSSNNA 202

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPF++LYQNP FP ++AF D+ GK      +NDKG  Y N+ DA+YDTL WSLK
Sbjct: 203 PFMVNIYPFLSLYQNPDFPIDFAFLDSSGKS-----INDKGKTYTNVFDANYDTLVWSLK 262

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
            +G  D+KI++GEVGWPTD N YAN  +AKRFY GL K +AS KGTPMRP+E  E+Y+F 
Sbjct: 263 KIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKGTPMRPDEKLEIYLFS 322

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
           L DE+ KSV PG FERHWGIFRYDG PKF MDL+  G +KMPVAAKGV+Y++ +WCVI +
Sbjct: 323 LFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAAKGVQYLEKKWCVIKN 382

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           ++ DL +   Q D  +AC  SDCT++ +G SCN+L ARGN S+ +NMY+Q Q+Q+ EACD
Sbjct: 383 SIEDLTSEVSQID--YACSLSDCTTLVFGSSCNDLNARGNFSFAYNMYFQMQDQSVEACD 442

Query: 422 FGGAAKIVT 430
           FGG+A+IVT
Sbjct: 443 FGGSAEIVT 443

[Close]

BLAST of MC01g0750 vs. ExPASy TrEMBL
Match: A0A6J1IB77 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111473519 PE=3 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 5.34e-193
Identity = 262/429 (61.07%), Postives = 333/429 (77.62%), Query Frame = 0


Query: 2   VSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVG 61
           V A   +GVNWGT+A++P++ SIVV LLKDNGI +VKLFD DS   +  LAG+ I+T+V 
Sbjct: 23  VEANLDVGVNWGTIASHPINPSIVVNLLKDNGINKVKLFDSDSWI-MGSLAGSKIETVVA 82

Query: 62  IRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPA 121
           I N+ LE FA DY+L KDWVK+N++TY+  GGVDI Y+AVGNEPFL ++ G Y+  TFPA
Sbjct: 83  IPNDQLEKFANDYDLAKDWVKENVTTYIYHGGVDIRYVAVGNEPFLTDFNGSYIKLTFPA 142

Query: 122 MQNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKS 181
           MQN+QKAL  AGH DKIKVTTPLN D+YE+P+N PSDGRFRS+I++ +K IV FL  + +
Sbjct: 143 MQNIQKALNDAGHGDKIKVTTPLNADVYESPSNQPSDGRFRSDIHDTMKNIVHFLSSNNA 202

Query: 182 PFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLK 241
           PFMVNIYPF++LYQNP FP ++AF D+ GK      +NDKG  Y N+ DA+YDTL WSLK
Sbjct: 203 PFMVNIYPFLSLYQNPHFPMDFAFLDSSGKS-----INDKGKTYTNVFDANYDTLVWSLK 262

Query: 242 DVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFG 301
            +G  D+KI++GEVGWPTD N YAN  +AKRFY GL K +AS KGTPMRP+E  E+Y+F 
Sbjct: 263 KIGEGDLKIVVGEVGWPTDANKYANLELAKRFYDGLFKNLASKKGTPMRPDEKLEIYLFS 322

Query: 302 LLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIAD 361
           L DE+ KSV PG FERHWGIFRYDG PKF MDL+  G +KMPVAAKGV+Y++ +WCVI +
Sbjct: 323 LFDEDMKSVLPGFFERHWGIFRYDGKPKFPMDLAGNGNDKMPVAAKGVQYLEKKWCVIKN 382

Query: 362 NVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACD 421
           ++ DL +   Q D  +AC  SDCT++ +G SCN+L ARGN S+ +NMY+Q Q+Q+ EACD
Sbjct: 383 SIEDLTSEVSQID--YACSLSDCTTLAFGSSCNDLNARGNFSFAYNMYFQMQDQSVEACD 442

Query: 422 FGGAAKIVT 430
           FGG+A+IVT
Sbjct: 443 FGGSAEIVT 443

[Close]

BLAST of MC01g0750 vs. TAIR 10
Match: AT5G64790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 474.2 bits (1219), Expect = 1.1e-133
Identity = 223/426 (52.35%), Postives = 308/426 (72.30%), Query Frame = 0


Query: 3   SAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVGI 62
           S  G  GVNWG +A++PL+ +IVVQ+LKDN I +VKLFD DS   ++ LAGTG++ +VGI
Sbjct: 23  SLVGGFGVNWGNIASHPLNPNIVVQMLKDNKINKVKLFDADS-WTMNALAGTGMEVMVGI 82

Query: 63  RNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPAM 122
            N LLE+ A DY+  KDWVK+N++ Y+ +GGVDI Y+AVGNEPFL  Y G ++ TTFPA+
Sbjct: 83  PNNLLESLADDYDNAKDWVKENVTQYIRKGGVDIKYVAVGNEPFLSAYNGSFLKTTFPAL 142

Query: 123 QNVQKALEAAGHSDKIKVTTPLNGDIYETPTNLPSDGRFRSNIYEPVKQIVQFLDQHKSP 182
           +N+ KAL+ AGH++ +K T P N ++Y++  + PS+G FR ++ + +  IV F  ++  P
Sbjct: 143 KNIHKALKEAGHTNIMKATIPQNAEVYQSANDKPSEGDFRKDVKQTMLDIVNFFHENDLP 202

Query: 183 FMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLKD 242
           F VNIYPF++LY N  FP  +AF D DG+      + DKG  Y N+ DA+YDTL ++LK 
Sbjct: 203 FTVNIYPFLSLYLNEHFPVEFAFLDGDGQT-----MTDKGKNYDNVFDANYDTLVYALKK 262

Query: 243 VGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFGL 302
            G+ DMKII+GEVGWPTDG+ YA+  +A++FY+GL+KR+A + GTP RP E  EVY+FG 
Sbjct: 263 AGIHDMKIIVGEVGWPTDGHKYASPKLAEKFYAGLMKRLAKDGGTPTRP-ERLEVYLFGF 322

Query: 303 LDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIADN 362
           LDE+ KS+ PG FERHWGIFRYDGTPKF +D + QG + +PVAAKGV+Y++ QWCV+  +
Sbjct: 323 LDEDMKSILPGPFERHWGIFRYDGTPKFMLDFTGQGRQMVPVAAKGVQYLEKQWCVVNKD 382

Query: 363 VTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACDF 422
             +L    +  D+ +AC + DCT+M  G +C+ L    N+SY FNMY+Q Q+Q+  ACDF
Sbjct: 383 TVNL--DEVGPDLDYACYHGDCTAMEAGSTCSKLTKVQNISYAFNMYFQIQDQDVRACDF 439

Query: 423 GGAAKI 429
            GAA I
Sbjct: 443 KGAAMI 439

[Close]

BLAST of MC01g0750 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 446.0 bits (1146), Expect = 3.3e-125
Identity = 218/432 (50.46%), Postives = 293/432 (67.82%), Query Frame = 0


Query: 1   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIV 60
           G+   G LGVNWGT+AT+ L    VVQ+LKDN I +VKLFD D    +  LAG+G++ +V
Sbjct: 19  GILVNG-LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADE-TTMGALAGSGLEVMV 78

Query: 61  GIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFP 120
            I N+ L+   T Y+  KDWV++N++ Y  +GGV+I ++AVGNEPFL +Y G ++N TFP
Sbjct: 79  AIPNDQLKVM-TSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFP 138

Query: 121 AMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTN--LPSDGRFRSNIYEPVKQIVQFLDQ 180
           A+ N+Q AL  AG  + +K T PLN D+Y++P +  +PS GRFR +I   + QIV FL +
Sbjct: 139 ALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGK 198

Query: 181 HKSPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTW 240
           + +P  +NIYPF++LY N  FP NYAFFD          +ND G  Y N+ DA++DTL  
Sbjct: 199 NNAPITINIYPFLSLYGNDDFPLNYAFFDGAEP------INDNGIDYTNVFDANFDTLVS 258

Query: 241 SLKDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVY 300
           SLK VG  DM II+GEVGWPT+G+ +AN   A RFY+GLL R+ +NKGTP+RP    EVY
Sbjct: 259 SLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTY-IEVY 318

Query: 301 MFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCV 360
           +FGLLDE+ KS+ PG FERHWGIF++DG PKF +DLS QG  K  + A+ V Y+ ++WC 
Sbjct: 319 LFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCT 378

Query: 361 IADNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPE 420
                 DL  + +  ++ +AC  SDCT++GYG SCN L A GN SY FNM++Q +NQ+  
Sbjct: 379 FNPEAKDL--TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDES 438

Query: 421 ACDFGGAAKIVT 431
           AC F G A I T
Sbjct: 439 ACYFQGLATITT 438

[Close]

BLAST of MC01g0750 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 446.0 bits (1146), Expect = 3.3e-125
Identity = 218/432 (50.46%), Postives = 293/432 (67.82%), Query Frame = 0


Query: 1   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIV 60
           G+   G LGVNWGT+AT+ L    VVQ+LKDN I +VKLFD D    +  LAG+G++ +V
Sbjct: 19  GILVNG-LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADE-TTMGALAGSGLEVMV 78

Query: 61  GIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFP 120
            I N+ L+   T Y+  KDWV++N++ Y  +GGV+I ++AVGNEPFL +Y G ++N TFP
Sbjct: 79  AIPNDQLKVM-TSYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTFP 138

Query: 121 AMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTN--LPSDGRFRSNIYEPVKQIVQFLDQ 180
           A+ N+Q AL  AG  + +K T PLN D+Y++P +  +PS GRFR +I   + QIV FL +
Sbjct: 139 ALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLGK 198

Query: 181 HKSPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTW 240
           + +P  +NIYPF++LY N  FP NYAFFD          +ND G  Y N+ DA++DTL  
Sbjct: 199 NNAPITINIYPFLSLYGNDDFPLNYAFFDGAEP------INDNGIDYTNVFDANFDTLVS 258

Query: 241 SLKDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVY 300
           SLK VG  DM II+GEVGWPT+G+ +AN   A RFY+GLL R+ +NKGTP+RP    EVY
Sbjct: 259 SLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTY-IEVY 318

Query: 301 MFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCV 360
           +FGLLDE+ KS+ PG FERHWGIF++DG PKF +DLS QG  K  + A+ V Y+ ++WC 
Sbjct: 319 LFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCT 378

Query: 361 IADNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPE 420
                 DL  + +  ++ +AC  SDCT++GYG SCN L A GN SY FNM++Q +NQ+  
Sbjct: 379 FNPEAKDL--TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDES 438

Query: 421 ACDFGGAAKIVT 431
           AC F G A I T
Sbjct: 439 ACYFQGLATITT 438

[Close]

BLAST of MC01g0750 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 445.3 bits (1144), Expect = 5.7e-125
Identity = 216/432 (50.00%), Postives = 292/432 (67.59%), Query Frame = 0


Query: 1   GVSAEGTLGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIV 60
           G+     LGVNWGT+AT+ L    VVQ+LKDN I +VKLFD D    +S L+G+G++ +V
Sbjct: 14  GIVMVDGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADE-TTMSALSGSGLEVMV 73

Query: 61  GIRNELLETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFP 120
            I N+ L+   + Y+  KDWV +N++ Y   GGV+I ++AVGNEPFL +Y G ++N TFP
Sbjct: 74  AIPNDQLKVMGS-YDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFP 133

Query: 121 AMQNVQKALEAAGHSDKIKVTTPLNGDIYETPTN--LPSDGRFRSNIYEPVKQIVQFLDQ 180
           A+QN+Q AL  AG    +K T PLN D+Y++P++  +PS GRFR +I   + QIV FL  
Sbjct: 134 ALQNIQNALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGN 193

Query: 181 HKSPFMVNIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTW 240
           + +P  +NIYPF++LY N  FP NYAFFD    +D      D G  Y N+ DA++DTL  
Sbjct: 194 NSAPITINIYPFLSLYGNDDFPLNYAFFDGAKPVD------DNGIAYTNVFDANFDTLVS 253

Query: 241 SLKDVGVTDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVY 300
           +LK VG  DM II+GEVGWPT+G+ +AN+  A RFY+GLL R+  N+GTP+RP    EVY
Sbjct: 254 ALKAVGHGDMPIIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTY-IEVY 313

Query: 301 MFGLLDENNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCV 360
           +FGLLDE+ KS+ PG FERHWGIF++DG PKF +DLS QG  K+ + A+ V Y   +WC+
Sbjct: 314 LFGLLDEDAKSIAPGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCM 373

Query: 361 IADNVTDLGASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPE 420
                 DL  + +  ++ +AC  SDCT++GYG SCN L A GN SY FNMY+Q +NQ+ +
Sbjct: 374 FNTEAKDL--TKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDED 433

Query: 421 ACDFGGAAKIVT 431
           AC F G A I T
Sbjct: 434 ACIFQGLATITT 434

[Close]

BLAST of MC01g0750 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 445.3 bits (1144), Expect = 5.7e-125
Identity = 221/427 (51.76%), Postives = 295/427 (69.09%), Query Frame = 0


Query: 8   LGVNWGTVATNPLDGSIVVQLLKDNGIKRVKLFDLDSAANLSVLAGTGIKTIVGIRNELL 67
           LGVNWGT++T+ L    VVQ+LKDN +K+VKLFD D+   +  LAG+GI+ +V I N+ L
Sbjct: 30  LGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADT-NTMVALAGSGIEVMVAIPNDQL 89

Query: 68  ETFATDYNLVKDWVKQNISTYMGEGGVDIIYIAVGNEPFLGNYQGKYVNTTFPAMQNVQK 127
           +   + YN  KDWV++NI+ +  +  V I Y+AVGNEPFL  Y G ++N T+PA+ N+QK
Sbjct: 90  KAMGS-YNRAKDWVRRNITRFNDD--VKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQK 149

Query: 128 ALEAAGHSDKIKVTTPLNGDIYETPTN--LPSDGRFRSNIYEPVKQIVQFLDQHKSPFMV 187
           AL  AG  D IK T PLN D+Y +P    +PS G FR +I+E +K IV FL  +K+PF V
Sbjct: 150 ALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEMKLIVNFLAHNKAPFTV 209

Query: 188 NIYPFINLYQNPGFPFNYAFFDNDGKLDKNSFVNDKGTIYYNMLDAHYDTLTWSLKDVGV 247
           NIYPF++LY +  FPF+YAFF+        + V+D G IY N+ DA++DTL  SLK +G 
Sbjct: 210 NIYPFLSLYLSSDFPFDYAFFNG------QNTVSDNGVIYTNVFDANFDTLLASLKALGH 269

Query: 248 TDMKIIIGEVGWPTDGNIYANTTVAKRFYSGLLKRMASNKGTPMRPNEDYEVYMFGLLDE 307
            DM +I+GEVGWPTDG+  AN   A+RFY+GLL ++A+N+GTPMRP    EVY+FG +DE
Sbjct: 270 GDMTVIVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPGY-IEVYLFGFIDE 329

Query: 308 NNKSVDPGNFERHWGIFRYDGTPKFAMDLSLQGVEKMPVAAKGVRYMKDQWCVIADNVTD 367
           + KS+ PGNFERHWGIF+YDG PKF  DLS  G +K+   A+ V+Y+++QWC+   N   
Sbjct: 330 DAKSIAPGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQWCMFNPNALT 389

Query: 368 L--GASNIQKDVSWACQNSDCTSMGYGCSCNNLGARGNVSYVFNMYYQTQNQNPEACDFG 427
                + +  +V++AC  SDCT++GYG SC NL   GN SY FNMY+Q QNQ  EACDF 
Sbjct: 390 FSNNTNQLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQVQNQKAEACDFE 445

Query: 428 GAAKIVT 431
           G A I T
Sbjct: 450 GLAIITT 445

The following BLAST results are available for this feature:

BLAST of MC01g0750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKW9	4.7e-124	50.46	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ...	[more]
Q9M088	2.9e-118	48.95	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ...	[more]
Q93Z08	4.7e-116	48.10	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ...	[more]
Q9FGH4	1.1e-80	37.47	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ...	[more]
Q9FJU9	3.2e-48	30.41	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]

BLAST of MC01g0750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133125.1	1.92e-280	86.28	glucan endo-1,3-beta-glucosidase 8-like, partial [Momordica charantia]	[more]
XP_022132538.1	8.59e-258	78.55	glucan endo-1,3-beta-glucosidase 5-like isoform X1 [Momordica charantia]	[more]
XP_022132539.1	6.34e-229	77.78	glucan endo-1,3-beta-glucosidase 5-like isoform X2 [Momordica charantia]	[more]
KAG7011052.1	9.22e-194	61.54	Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022927955.1	9.22e-194	61.54	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata]	[more]

BLAST of MC01g0750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BUF0	9.28e-281	86.28	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005798 ...	[more]
A0A6J1BWJ0	4.16e-258	78.55	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 ...	[more]
A0A6J1BTC0	3.07e-229	77.78	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111005372 ...	[more]
A0A6J1EQB3	4.46e-194	61.54	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111434805 P...	[more]
A0A6J1IB77	5.34e-193	61.07	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111473519 PE=...	[more]

BLAST of MC01g0750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64790.1	1.1e-133	52.35	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.1	3.3e-125	50.46	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.2	3.3e-125	50.46	O-Glycosyl hydrolases family 17 protein	[more]
AT2G19440.1	5.7e-125	50.00	O-Glycosyl hydrolases family 17 protein	[more]
AT5G18220.1	5.7e-125	51.76	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 355..430 e-value: 1.4E-18 score: 77.8
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 355..426 e-value: 4.7E-16 score: 59.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 342..430 e-value: 3.6E-6 score: 29.1
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 7..341 e-value: 1.7E-105 score: 354.5
None	No IPR available	PANTHER	PTHR32227:SF290	BETA-1,3-GLUCANASE	coord: 6..430
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 8..333 e-value: 6.6E-61 score: 206.6
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 248..261
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 6..430
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 8..333

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0750.1	MC01g0750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0008033	tRNA processing
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds