MC01g0705 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g0705 vs. ExPASy Swiss-Prot
Match: F4KF14 (Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FTSHI4 PE=1 SV=1)

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 662/860 (76.98%), Postives = 753/860 (87.56%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           MTF  S++   T  S P+ PS T        S  S       + AK+ ++  +F  ++  
Sbjct: 1   MTFYISSSLTPTHFSKPLNPSNTLFPSQFRGSLSSFVRRRKPTEAKLSSKFNLFPSRR-- 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
                 T  S+S  ++  +S + EED ES +LFEKL+  ER+R++ +EEL+RKAN+QLER
Sbjct: 61  --NGLITCCSTSSFESTESSVSQEEDAESNRLFEKLRETERERLSNMEELERKANVQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMAS WSR LLTMRGKLKGTEWDPE SH+INFSDF  LL+S++VQ++EYSNYGQT+SV
Sbjct: 121 QLVMASDWSRTLLTMRGKLKGTEWDPETSHRINFSDFMKLLDSNSVQYMEYSNYGQTISV 180

Query: 181 ILPYYKD----GTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPA 240
           ILPYYKD    G E +++ EIIFRRH++DRMPID WNDVWKKLHQQIVNV+V NVD VPA
Sbjct: 181 ILPYYKDGEPLGEEEDSKKEIIFRRHIVDRMPIDGWNDVWKKLHQQIVNVEVFNVDVVPA 240

Query: 241 EVYSSVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGS 300
           EVY++VAT VVWSMRLAL V+LY+WID++ RPIYAKLIPCDLG P K  R PLKR+ALGS
Sbjct: 241 EVYTTVATFVVWSMRLALFVSLYVWIDSITRPIYAKLIPCDLGTPTKKIRQPLKRQALGS 300

Query: 301 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHG 360
           LGKSRAKFISAEE TGVTF DFAGQEYIKRELQEIVRILKNDEEFQ+KGIYCPKGVLLHG
Sbjct: 301 LGKSRAKFISAEEKTGVTFDDFAGQEYIKRELQEIVRILKNDEEFQNKGIYCPKGVLLHG 360

Query: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 420
           PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFAS+RSYAPSIIFIDE
Sbjct: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASSRSYAPSIIFIDE 420

Query: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 480
           IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKV+T+QVLVIGATNRLDILDPALLRK
Sbjct: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVTTSQVLVIGATNRLDILDPALLRK 480

Query: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILN 540
           GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSE+ KE LLQE+AE TEDFTGAELQN+LN
Sbjct: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEDEKEELLQEVAENTEDFTGAELQNVLN 540

Query: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 600
           EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTE+PEELKLRLAYREAAVA+LAC
Sbjct: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEVPEELKLRLAYREAAVAVLAC 600

Query: 601 YFPDTHRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVD 660
           Y PD +RP  ETDI SIRSQPNM Y+ET GRVFARK DYVNSI+RACAPRV+EEEMFG++
Sbjct: 601 YLPDQYRPISETDINSIRSQPNMRYSETSGRVFARKSDYVNSIIRACAPRVVEEEMFGIE 660

Query: 661 NLCWISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAV 720
           NLCWIS+K+TLEAS+ AEFLILQTGMTAFGKAYYRN  DLVPNL  KLEALR+EY+R+AV
Sbjct: 661 NLCWISAKSTLEASQRAEFLILQTGMTAFGKAYYRNQRDLVPNLVPKLEALRDEYMRFAV 720

Query: 721 EKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAG 780
           EKCSSIL+EY SA+E ITD+LLEKGEI+A+EIW+IY  APR PQ  VRPVDE+GAL+YAG
Sbjct: 721 EKCSSILQEYQSALEEITDVLLEKGEIKADEIWNIYNTAPRIPQKPVRPVDEYGALIYAG 780

Query: 781 RWGIYGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAE 840
           RWGI+GV+LPGRVTF+PGN GFATFGAPRPMETQ+++D+TWKL+D+IW+K+V+EI+  AE
Sbjct: 781 RWGIHGVSLPGRVTFSPGNIGFATFGAPRPMETQIISDDTWKLVDEIWDKKVEEIK--AE 840

Query: 841 ASAEVEEDKEKPQLLMASHF 857
           A  ++EE+K+KPQ+LMA+HF
Sbjct: 841 AVIQIEEEKKKPQILMATHF 854

BLAST of MC01g0705 vs. ExPASy Swiss-Prot
Match: O19922 (ATP-dependent zinc metalloprotease FtsH OS=Cyanidium caldarium OX=2771 GN=ftsH PE=3 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 6.7e-82
Identity = 191/473 (40.38%), Postives = 271/473 (57.29%), Query Frame = 0

Query: 296 SLGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLH 355
           + GK++A+F   E  TG+ F D AG E  K ELQEIV  LK+  +F + G   PKGVLL 
Sbjct: 152 NFGKAKARF-HMEAKTGIVFEDVAGIEEAKEELQEIVAFLKDSRKFTNVGATIPKGVLLV 211

Query: 356 GPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFID 415
           GPPGTGKTLLAKAIAGEA  PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP I+FID
Sbjct: 212 GPPGTGKTLLAKAIAGEASAPFFSISGSEFVEMFVGVGASRVRDLFKKAKEKAPCIVFID 271

Query: 416 EIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLR 475
           EIDA+G +R G  IGGG  EREQ L Q+LTEMDGF   T  V+V+ ATNR+D+LD ALLR
Sbjct: 272 EIDAVGRQR-GVGIGGGNDEREQTLNQLLTEMDGFSGDTG-VIVVAATNRIDVLDSALLR 331

Query: 476 KGRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNIL 535
            GRFD+ I V LP+ +GRLAILKVH++ K       K+ LL+ IA  T  F+GA+L N+L
Sbjct: 332 PGRFDRQIMVSLPNINGRLAILKVHSKKKKIH----KDVLLEVIARRTPGFSGADLANLL 391

Query: 536 NEAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILA 595
           NEA ILT R+    I  +E+ +++ +       G E S      +K  +AY EA  A+ A
Sbjct: 392 NEAAILTVRRGKVEITMKEIEDSIDK----IIAGLEGSPLADSRIKRLIAYHEAGHAVAA 451

Query: 596 CYFPDTHRPFIETDIKSIRSQPNM-HYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFG 655
            + P  H P  +  +   R    +  +     +    K   ++ I+ A A R +EE +FG
Sbjct: 452 TFLPH-HDPVQKVTLIPRRQAKGLTWFLPNDDQFLVSKSQILSKIIAALAGRAMEEIVFG 511

Query: 656 VDNLCWISSKATLEASKLAEFLILQTGMTAFGKAYYRNHG-------------DLVPNLA 715
           +  +   ++    + + +A  ++ + GM+  G     N               +L   + 
Sbjct: 512 LPEVTIGAANDIKQVTFMARQMVTKFGMSKVGPICLENSSSEVFIGRDLMGRHELSEEMV 571

Query: 716 SKLEALREEYLRYAVEKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEK 755
           +K++      L+    +  +IL +    ++ + + L+EK  I A+E   I E+
Sbjct: 572 AKVDLEVRSILKDCYIQARTILSQNRKLIDRVVNELVEKETIEAKEFMRIVEE 612

BLAST of MC01g0705 vs. ExPASy Swiss-Prot
Match: P51327 (ATP-dependent zinc metalloprotease FtsH OS=Porphyra purpurea OX=2787 GN=ftsH PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 1.1e-79
Identity = 192/479 (40.08%), Postives = 269/479 (56.16%), Query Frame = 0

Query: 296 SLGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLH 355
           S GKS+A F   E  TGV F+D AG E  K E QE+V  LK  E F   G   PKGVLL 
Sbjct: 154 SFGKSKALF-QMEAKTGVVFNDVAGVEEAKEEFQEVVTFLKQPESFTAVGAKIPKGVLLV 213

Query: 356 GPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFID 415
           GPPGTGKTLLAKAIAGEAG+PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP I+FID
Sbjct: 214 GPPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKDNAPCIVFID 273

Query: 416 EIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLR 475
           EIDA+G +R G  +GGG  EREQ L Q+LTEMDGF+ +T  V+VI ATNR DILD ALLR
Sbjct: 274 EIDAVGRQR-GTGVGGGNDEREQTLNQLLTEMDGFEGNTG-VIVIAATNRADILDSALLR 333

Query: 476 KGRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNIL 535
            GRFD+ + V +P   GRLAIL+VHA+NK   S    +  L+ IA  T  F+GA+L N+L
Sbjct: 334 PGRFDRQVSVDVPDFRGRLAILEVHAKNKKMES----KVSLETIARRTPGFSGADLANLL 393

Query: 536 NEAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILA 595
           NEA ILTAR+    +   E+  ++ R       G E +  I  + K  +AY E   AI+ 
Sbjct: 394 NEAAILTARRRKSAMTMSEIDTSIDR----VVAGLEGTPLIDSKSKRLIAYHEVGHAIIG 453

Query: 596 CYFPDTHRPFIE-TDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFG 655
               + H P  + T I   +++    +  +  +    +   +  IV A   R  EE +FG
Sbjct: 454 SLL-EHHDPVQKVTLIPRGQARGLTWFTPSDDQSLISRSQILARIVGALGGRAAEEIIFG 513

Query: 656 VDNLCWISSKATLEASKLAEFLILQTGMTAFGKAYYRNHG-------------DLVPNLA 715
              +   +S    + + +A  ++ + GM+  G     + G             +    +A
Sbjct: 514 DAEVTTGASNDLQQVTSMARQMVTRFGMSKIGPLSLESQGSDPFLGRGMGGGSEYSDEVA 573

Query: 716 SKLEALREEYLRYAVEKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQ 761
           + ++    E +    ++   I+++    ++ + D+L+EK  I   E  HI ++    P+
Sbjct: 574 TNIDKQVREIVSECYKEAKKIVKDNRVVMDRLVDLLIEKETIEGNEFRHIVKEYTAIPE 620

BLAST of MC01g0705 vs. ExPASy Swiss-Prot
Match: Q55700 (ATP-dependent zinc metalloprotease FtsH 2 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=ftsH2 PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 3.1e-79
Identity = 199/513 (38.79%), Postives = 279/513 (54.39%), Query Frame = 0

Query: 261 WIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKSRAKFISAEETTGVTFSDFAG 320
           ++ NL  P+   LI     + R+ + +P       + GKS+A+F   +  TGV F D AG
Sbjct: 120 FVGNLIFPVL--LIASLFFLFRRSSNMPGGPGQAMNFGKSKARF-QMDAKTGVMFDDVAG 179

Query: 321 QEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGTGKTLLAKAIAGEAGLPFFAA 380
            +  K ELQE+V  LK  E F   G   PKGVLL GPPGTGKTLLAKAIAGEAG+PFF+ 
Sbjct: 180 IDEAKEELQEVVTFLKQPERFTAVGAKIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSI 239

Query: 381 NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAIGSKRGGPDIGGGGAEREQGL 440
           +G++FVEMFVGV ASRV+DLF  A+  AP +IFIDEIDA+G +RG   IGGG  EREQ L
Sbjct: 240 SGSEFVEMFVGVGASRVRDLFKKAKENAPCLIFIDEIDAVGRQRGA-GIGGGNDEREQTL 299

Query: 441 LQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFDKIIRVGLPSKDGRLAILKVH 500
            Q+LTEMDGF+ +T  +++I ATNR D+LD AL+R GRFD+ + V  P   GR  IL+VH
Sbjct: 300 NQLLTEMDGFEGNTG-IIIIAATNRPDVLDSALMRPGRFDRQVMVDAPDYSGRKEILEVH 359

Query: 501 ARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGILTARKDLDYIGREELLEALK 560
           ARNK    +   E  +  IA  T  F+GA+L N+LNEA ILTAR+    I   E+ +A+ 
Sbjct: 360 ARNK----KLAPEVSIDSIARRTPGFSGADLANLLNEAAILTARRRKSAITLLEIDDAVD 419

Query: 561 RQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPDTHRPFIE-TDIKSIRSQPNM 620
           R       G E +  +  + K  +AY E   AI+     D H P  + T I   ++Q   
Sbjct: 420 R----VVAGMEGTPLVDSKSKRLIAYHEVGHAIVGTLLKD-HDPVQKVTLIPRGQAQGLT 479

Query: 621 HYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCWISSKATLEASKLAEFLILQ 680
            +     +    K   +  I  A   R  EEE+FG D +   +     + +++A  ++ +
Sbjct: 480 WFTPNEEQGLTTKAQLMARIAGAMGGRAAEEEVFGDDEVTTGAGGDLQQVTEMARQMVTR 539

Query: 681 TGMTAFGKAYYRNHGDLV-------------PNLASKLEALREEYLRYAVEKCSSILREY 740
            GM+  G     + G  V               +A++++A   +      +    I++E 
Sbjct: 540 FGMSNLGPISLESSGGEVFLGGGLMNRSEYSEEVATRIDAQVRQLAEQGHQMARKIVQEQ 599

Query: 741 HSAVETITDILLEKGEIRAEEIWHIYEKAPRFP 760
              V+ + D+L+EK  I  EE   I  +    P
Sbjct: 600 REVVDRLVDLLIEKETIDGEEFRQIVAEYAEVP 618

BLAST of MC01g0705 vs. ExPASy Swiss-Prot
Match: B9KXV3 (ATP-dependent zinc metalloprotease FtsH 1 OS=Thermomicrobium roseum (strain ATCC 27502 / DSM 5159 / P-2) OX=309801 GN=ftsH1 PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 2.0e-78
Identity = 203/497 (40.85%), Postives = 277/497 (55.73%), Query Frame = 0

Query: 296 SLGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLH 355
           S GKSRA+  ++   T VTF D AG +  K ELQEIV  LK  E+F   G   P+GVLL 
Sbjct: 144 SFGKSRARVFTSNRPT-VTFDDVAGVDEAKEELQEIVEFLKYPEKFAALGARIPRGVLLV 203

Query: 356 GPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFID 415
           GPPGTGKTLL++A+AGEAG+PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP I+FID
Sbjct: 204 GPPGTGKTLLSRAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNAPCIVFID 263

Query: 416 EIDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLR 475
           EIDA+G +RG   +GG   EREQ L QIL EMDGF  ST  V+VI ATNR D+LDPALLR
Sbjct: 264 EIDAVGRQRGA-GLGGSHDEREQTLNQILVEMDGFDSST-NVIVIAATNRPDVLDPALLR 323

Query: 476 KGRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNIL 535
            GRFD+ + +  P   GRLAILKVH R K   S    +  L+++A  T  F+GA+L+N++
Sbjct: 324 PGRFDRQVVLDRPDLHGRLAILKVHTRGKPLES----DVDLEDLARQTPGFSGADLENLV 383

Query: 536 NEAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILA 595
           NEA IL AR++   IGR EL EA+ R        +  S  I E  KL  AY EA  A++A
Sbjct: 384 NEAAILAARRNKKTIGRRELYEAIDR---VVAGPERKSRRISEREKLMTAYHEAGHALVA 443

Query: 596 CYFPDTHRPFIETDIKSIRSQPNMHYAETP--GRVFARKIDYVNSIVRACAPRVIEEEMF 655
              P    P  +  I + R     +    P   R F  K  +   +    A  V EE +F
Sbjct: 444 RMLPHAD-PVHKVSIVA-RGMMGGYTRVLPEEDRFFWTKKQFEAQLAVFMAGLVAEELVF 503

Query: 656 GVDNLCWISSKATLEASKLAEFLILQTGMT------AFGK-----------AYYRNHGDL 715
               +   ++     A+ LA  ++ + GM+      AFG+           A  RN+ D 
Sbjct: 504 --QEVSTGAANDIERATTLARRMVTEFGMSERLGPLAFGRKEELVFLGREIAEQRNYSDQ 563

Query: 716 VPNLASKLEALREEYLRYAVEKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEKAP 774
           V   A +++      +  A +    IL ++   +E I  +L+EK  +   EI  ++++  
Sbjct: 564 V---AYEIDQEVRRLIDQAYQTAKQILLDHMDKLEKIATLLVEKETLDGHEIEALFDEPR 623

BLAST of MC01g0705 vs. NCBI nr
Match: XP_022132617.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Momordica charantia])

HSP 1 Score: 1657 bits (4290), Expect = 0.0
Identity = 857/857 (100.00%), Postives = 857/857 (100.00%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR
Sbjct: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER
Sbjct: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW
Sbjct: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS
Sbjct: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI
Sbjct: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEEDKEKPQLLMASHFL
Sbjct: 841 VEEDKEKPQLLMASHFL 857

BLAST of MC01g0705 vs. NCBI nr
Match: XP_038881869.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Benincasa hispida] >XP_038881871.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Benincasa hispida] >XP_038881872.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 1553 bits (4022), Expect = 0.0
Identity = 800/857 (93.35%), Postives = 827/857 (96.50%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA NF Q SHP+LPSTTS+GRIQLV SCSSS SNH SA K R+R+R F+F K R
Sbjct: 1   MKFMASNALNFMQFSHPILPSTTSLGRIQLVPSCSSSCSNHSSATKTRSRDRNFTFHKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +AS+SDTD VVAS NVEEDVESAQLFEKLK AERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASNSDTDLVVASPNVEEDVESAQLFEKLKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD T G+A+NEIIFRRHVIDRMPIDCWNDVWKKLHQQ+VNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDETSGSAKNEIIFRRHVIDRMPIDCWNDVWKKLHQQVVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAV+WSMRLALSVALYLWIDNL RP YAKLIPCDLGVP+KITRLPLKRRALGSLGKS
Sbjct: 241 SVATAVIWSMRLALSVALYLWIDNLTRPTYAKLIPCDLGVPKKITRLPLKRRALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDE+FQD+GIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEDFQDRGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFF+SEE KEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFQSEENKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQ+GTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQRGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIET+IKSIRSQPNM YAETPGRVF+RK+DYVNSIVRACAPRVIEEEMFGVDNLCW
Sbjct: 601 THRPFIETNIKSIRSQPNMRYAETPGRVFSRKLDYVNSIVRACAPRVIEEEMFGVDNLCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATLEASKLAEFLILQTGMTAFGKAYYRN GDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNPGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           S+LREYHSAVETITDILLE+GEI+AEEIW I+EK PRFPQP+VRP+DEHGALLYAGRWGI
Sbjct: 721 SVLREYHSAVETITDILLEEGEIQAEEIWDIFEKRPRFPQPSVRPIDEHGALLYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEI+AEA  SAE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIKAEA--SAE 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEEDKEKPQLLMASHFL
Sbjct: 841 VEEDKEKPQLLMASHFL 855

BLAST of MC01g0705 vs. NCBI nr
Match: XP_022977393.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima] >XP_022977394.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima] >XP_022977395.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima] >XP_022977396.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1544 bits (3997), Expect = 0.0
Identity = 795/857 (92.77%), Postives = 822/857 (95.92%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA N  QLSHP LPSTTS+GRIQLVSSCSSS SNHGS  K R+R+RIF+F K R
Sbjct: 1   MMFMASNALNLMQLSHPALPSTTSLGRIQLVSSCSSSCSNHGSPTKTRSRDRIFTFPKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAV+ASTNVEEDVESAQLFEKLK  ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNS+NVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSNNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGT GNA+NE+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFI+TDIKSIRSQPNMHYAE PGRVF+RKIDYVNSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIKTDIKSIRSQPNMHYAEIPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATL+ASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           S+LREYHSAVETITD+LLEKGEIRAEEI  IYEKAPRFPQPAVRP+DEHGAL+YAGRWGI
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPAVRPIDEHGALMYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGN GF  FGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEA  SA+
Sbjct: 781 YGVTLPGRVTFAPGNVGFVNFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEA--SAD 840

Query: 841 VEEDKEKPQLLMASHFL 857
           V ED+EKPQLLMASHFL
Sbjct: 841 VVEDEEKPQLLMASHFL 855

BLAST of MC01g0705 vs. NCBI nr
Match: XP_022950306.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita moschata] >XP_022950307.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita moschata] >XP_022950308.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita moschata] >XP_022950309.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1539 bits (3985), Expect = 0.0
Identity = 793/857 (92.53%), Postives = 821/857 (95.80%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA N  QLSHPVLPSTTS+GRIQLVSSCSSS +NHGS  K R+R+RIF+F K R
Sbjct: 1   MMFMASNALNLMQLSHPVLPSTTSLGRIQLVSSCSSSCANHGSPTKTRSRDRIFTFPKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAV+ASTNVEEDVESAQLFEKLK  ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDF TLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFLTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGT GNA+NE+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAV+WSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVIWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLD+IGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDHIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETDIKSIRSQPNM YAE PGRVF+RKIDY+NSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIETDIKSIRSQPNMRYAEIPGRVFSRKIDYINSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATL+ASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           S+LREYHSAVETITD+LLEKGEIRAEEI  IYEKAPRFPQP VRP+DEHGAL+YAGRWGI
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPTVRPIDEHGALMYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGN GF TFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAE   SA+
Sbjct: 781 YGVTLPGRVTFAPGNVGFVTFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEV--SAD 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEEDKEKPQLLMASHFL
Sbjct: 841 VEEDKEKPQLLMASHFL 855

BLAST of MC01g0705 vs. NCBI nr
Match: XP_023545057.1 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023545058.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1533 bits (3970), Expect = 0.0
Identity = 791/857 (92.30%), Postives = 819/857 (95.57%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA N  QLSHPVLPSTTS+GRIQLVSSCSSS SNHGS  K R+R+RIF+F K R
Sbjct: 1   MMFMASNALNLMQLSHPVLPSTTSLGRIQLVSSCSSSCSNHGSPTKTRSRDRIFTFPKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSD DAV+ASTNVEEDVESAQLFEKLK  ERQRIN LEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDIDAVIASTNVEEDVESAQLFEKLKDDERQRINNLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDF TLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFLTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGT GNA+NE+IFRRHV+DRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVVDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAV+WSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVIWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYC KGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCQKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLD+IGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDHIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETDIKSIRSQPNM YAE PGRVF+RKIDY+NSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIETDIKSIRSQPNMRYAEIPGRVFSRKIDYINSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATL+ASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           S+LREYHSAVETITD+LLEKGEIRAEEI  IYEKAPRFPQPAVRP+DEHGALLYAGRWGI
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPAVRPIDEHGALLYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGN GF TFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAE   SA+
Sbjct: 781 YGVTLPGRVTFAPGNVGFVTFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEV--SAD 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEED+EKPQLLMASHFL
Sbjct: 841 VEEDEEKPQLLMASHFL 855

BLAST of MC01g0705 vs. ExPASy TrEMBL
Match: A0A6J1BUB8 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111005436 PE=4 SV=1)

HSP 1 Score: 1657 bits (4290), Expect = 0.0
Identity = 857/857 (100.00%), Postives = 857/857 (100.00%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR
Sbjct: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER
Sbjct: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW
Sbjct: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS
Sbjct: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI
Sbjct: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE
Sbjct: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEEDKEKPQLLMASHFL
Sbjct: 841 VEEDKEKPQLLMASHFL 857

BLAST of MC01g0705 vs. ExPASy TrEMBL
Match: A0A6J1IPV0 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111477742 PE=4 SV=1)

HSP 1 Score: 1544 bits (3997), Expect = 0.0
Identity = 795/857 (92.77%), Postives = 822/857 (95.92%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA N  QLSHP LPSTTS+GRIQLVSSCSSS SNHGS  K R+R+RIF+F K R
Sbjct: 1   MMFMASNALNLMQLSHPALPSTTSLGRIQLVSSCSSSCSNHGSPTKTRSRDRIFTFPKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAV+ASTNVEEDVESAQLFEKLK  ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDFWTLLNS+NVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFWTLLNSNNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGT GNA+NE+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFI+TDIKSIRSQPNMHYAE PGRVF+RKIDYVNSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIKTDIKSIRSQPNMHYAEIPGRVFSRKIDYVNSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATL+ASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           S+LREYHSAVETITD+LLEKGEIRAEEI  IYEKAPRFPQPAVRP+DEHGAL+YAGRWGI
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPAVRPIDEHGALMYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGN GF  FGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEA  SA+
Sbjct: 781 YGVTLPGRVTFAPGNVGFVNFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEA--SAD 840

Query: 841 VEEDKEKPQLLMASHFL 857
           V ED+EKPQLLMASHFL
Sbjct: 841 VVEDEEKPQLLMASHFL 855

BLAST of MC01g0705 vs. ExPASy TrEMBL
Match: A0A6J1GEJ5 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111453429 PE=4 SV=1)

HSP 1 Score: 1539 bits (3985), Expect = 0.0
Identity = 793/857 (92.53%), Postives = 821/857 (95.80%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA N  QLSHPVLPSTTS+GRIQLVSSCSSS +NHGS  K R+R+RIF+F K R
Sbjct: 1   MMFMASNALNLMQLSHPVLPSTTSLGRIQLVSSCSSSCANHGSPTKTRSRDRIFTFPKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAV+ASTNVEEDVESAQLFEKLK  ERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVIASTNVEEDVESAQLFEKLKDDERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMASSWSRALLTMRGKLKGTEWDPENSH+INFSDF TLLNSSNVQFIEYSNYGQTMSV
Sbjct: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHRINFSDFLTLLNSSNVQFIEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKDGT GNA+NE+IFRRHVIDRMPIDCWNDVWK+LHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDGTGGNAKNEVIFRRHVIDRMPIDCWNDVWKRLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAV+WSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP K TR PL+R+ LGSLGKS
Sbjct: 241 SVATAVIWSMRLALSVALYLWIDNLMRPIYAKLIPCDLGVPTKTTRPPLQRQTLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTG+TFSDFAGQEYIKRELQEIVRILKNDE FQ+KGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGITFSDFAGQEYIKRELQEIVRILKNDETFQEKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFA NGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAVNGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKDLD+IGREELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILA YFPD
Sbjct: 541 LTARKDLDHIGREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILASYFPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THRPFIETDIKSIRSQPNM YAE PGRVF+RKIDY+NSIVRACAPRVIEEEMFGVDN+CW
Sbjct: 601 THRPFIETDIKSIRSQPNMRYAEIPGRVFSRKIDYINSIVRACAPRVIEEEMFGVDNMCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKATL+ASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATLDASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           S+LREYHSAVETITD+LLEKGEIRAEEI  IYEKAPRFPQP VRP+DEHGAL+YAGRWGI
Sbjct: 721 SVLREYHSAVETITDVLLEKGEIRAEEIRDIYEKAPRFPQPTVRPIDEHGALMYAGRWGI 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGN GF TFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAE   SA+
Sbjct: 781 YGVTLPGRVTFAPGNVGFVTFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEV--SAD 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEEDKEKPQLLMASHFL
Sbjct: 841 VEEDKEKPQLLMASHFL 855

BLAST of MC01g0705 vs. ExPASy TrEMBL
Match: A0A1S4DU96 (probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485274 PE=4 SV=1)

HSP 1 Score: 1503 bits (3892), Expect = 0.0
Identity = 776/857 (90.55%), Postives = 812/857 (94.75%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           M FMASNA N  QLSHP+LPSTTS+GRIQL+ SCSSS SNHGSA K R+R+R  +F K R
Sbjct: 1   MKFMASNALNSIQLSHPILPSTTSLGRIQLLPSCSSSCSNHGSATKTRSRHRSITFPKFR 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           + RV T +ASSSDTDAVVAS NVEED ESAQLFEKLK AERQRINKLEELQRKANLQLER
Sbjct: 61  QHRVFTPKASSSDTDAVVASANVEEDDESAQLFEKLKDAERQRINKLEELQRKANLQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLV+ASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQF+EYSNYGQTMSV
Sbjct: 121 QLVLASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFVEYSNYGQTMSV 180

Query: 181 ILPYYKDGTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240
           ILPYYKD T G+A+NEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS
Sbjct: 181 ILPYYKDETGGSAKNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPAEVYS 240

Query: 241 SVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGSLGKS 300
           SVATAVVWSMRLALSVALYLWIDNL RPIYAKLIPCDLGVP+ +  LP+KR  LGSLGKS
Sbjct: 241 SVATAVVWSMRLALSVALYLWIDNLTRPIYAKLIPCDLGVPKTLPNLPIKRSLLGSLGKS 300

Query: 301 RAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGPPGT 360
           RAKFISAEETTGV+FSDFAGQEYIKRELQEIV+ILKNDE+FQDKGIYCPKGVLLHGPPGT
Sbjct: 301 RAKFISAEETTGVSFSDFAGQEYIKRELQEIVQILKNDEDFQDKGIYCPKGVLLHGPPGT 360

Query: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420
           GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI
Sbjct: 361 GKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEIDAI 420

Query: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480
           GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD
Sbjct: 421 GSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKGRFD 480

Query: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNEAGI 540
           KIIRVGLPSKDGRLAILKVHARNKFFRSEE KEALL+EIAELTEDFTGAELQNILNEAGI
Sbjct: 481 KIIRVGLPSKDGRLAILKVHARNKFFRSEENKEALLEEIAELTEDFTGAELQNILNEAGI 540

Query: 541 LTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACYFPD 600
           LTARKD DYI REELLEALKRQKGTFETGQEDSTEIPE+LKLRLAYREAAVAILACY PD
Sbjct: 541 LTARKDQDYIEREELLEALKRQKGTFETGQEDSTEIPEDLKLRLAYREAAVAILACYLPD 600

Query: 601 THRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVDNLCW 660
           THR FIET+IKSIRS+PNMHY ETPGRVF+RKIDYVNSIVRACAPRVIEEEMFG+DNLCW
Sbjct: 601 THRSFIETNIKSIRSRPNMHYDETPGRVFSRKIDYVNSIVRACAPRVIEEEMFGIDNLCW 660

Query: 661 ISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAVEKCS 720
           ISSKAT EA+KLA+ LILQTGMTAFGKAYYR+ GDLVPNLA KLEALREEYLRYAVEKC 
Sbjct: 661 ISSKATTEAAKLADLLILQTGMTAFGKAYYRHPGDLVPNLAPKLEALREEYLRYAVEKCF 720

Query: 721 SILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAGRWGI 780
           SILREYHSAVETITDILLEK  I+A EIW I+EKAPR PQP+VRP+DEHGALLYAGRWG+
Sbjct: 721 SILREYHSAVETITDILLEKEVIKAAEIWDIFEKAPRLPQPSVRPIDEHGALLYAGRWGV 780

Query: 781 YGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAEASAE 840
           YGVTLPGRVTFAPGNAG+ATFGAPRPMETQVVNDETWKLID+IW+KRVQEIRA+A  S E
Sbjct: 781 YGVTLPGRVTFAPGNAGYATFGAPRPMETQVVNDETWKLIDNIWDKRVQEIRAKA--SEE 840

Query: 841 VEEDKEKPQLLMASHFL 857
           VEEDKEKPQLLMASHFL
Sbjct: 841 VEEDKEKPQLLMASHFL 855

BLAST of MC01g0705 vs. ExPASy TrEMBL
Match: A0A5B7BKE7 (AAA domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_037084 PE=4 SV=1)

HSP 1 Score: 1372 bits (3552), Expect = 0.0
Identity = 704/861 (81.77%), Postives = 776/861 (90.13%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           MT   SN F+F +   P  PS T  G   L       SS H   + +R +     F K  
Sbjct: 1   MTSNVSNTFDFCKFPKPFSPSKTLDGPFLL-------SSRHVFRSNLRLKTNTALFVKPN 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
           +L +   RASSS++++VV ST  EED ESAQLFEKLK AE++RINKLEEL+RKAN+QLER
Sbjct: 61  KLNIRGCRASSSNSNSVVPSTVQEEDAESAQLFEKLKDAEKERINKLEELERKANVQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMAS WSRALLTMRGKLKGTEW+PENSH+I++S+F  LL+S+NVQF+EYSNYGQT+SV
Sbjct: 121 QLVMASDWSRALLTMRGKLKGTEWEPENSHRIDYSEFRRLLDSNNVQFMEYSNYGQTVSV 180

Query: 181 ILPYYKDG----TEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPA 240
           ILPYYKDG    +EG+ + EI+FRRHV+DRMPIDCWNDVW+KLHQQ++NVDV+NV+ VPA
Sbjct: 181 ILPYYKDGKIEASEGDLKKEIVFRRHVVDRMPIDCWNDVWRKLHQQLINVDVLNVNTVPA 240

Query: 241 EVYSSVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGS 300
           EVYS+VATAVVWSMRLALS+ LYLWIDN+ RPIYAKLIPCDLG P + TR PLKRRALGS
Sbjct: 241 EVYSTVATAVVWSMRLALSIVLYLWIDNMMRPIYAKLIPCDLGTPPEKTRQPLKRRALGS 300

Query: 301 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHG 360
           LGKSRAKFISAEETTGVTF+DFAGQEYIKRELQEIVRILKNDEEFQ+KGIYCPKGVLLHG
Sbjct: 301 LGKSRAKFISAEETTGVTFNDFAGQEYIKRELQEIVRILKNDEEFQEKGIYCPKGVLLHG 360

Query: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 420
           PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARS+APSIIFIDE
Sbjct: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSFAPSIIFIDE 420

Query: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 480
           IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVST+QVLVIGATNRLDILDPALLRK
Sbjct: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTSQVLVIGATNRLDILDPALLRK 480

Query: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILN 540
           GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEE KE LLQEIAELTEDFTGAELQNILN
Sbjct: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEEKETLLQEIAELTEDFTGAELQNILN 540

Query: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 600
           EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVA+LAC
Sbjct: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAVLAC 600

Query: 601 YFPDTHRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVD 660
           YFPD +RPF ETDI +I SQPNM Y ETPGRVF RK DYVNSIV ACAPRVIEEEMFGVD
Sbjct: 601 YFPDPYRPFTETDINAIHSQPNMRYVETPGRVFKRKADYVNSIVCACAPRVIEEEMFGVD 660

Query: 661 NLCWISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAV 720
           NLCWIS+KATL+AS+LAE LILQTGMTAFGKAYYRN  DLVPNLA+KLEALR+EY+++AV
Sbjct: 661 NLCWISAKATLDASRLAEILILQTGMTAFGKAYYRNLNDLVPNLAAKLEALRDEYMQFAV 720

Query: 721 EKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAG 780
           EKCSS+LREYHSAVETITDILLEKGEI+A+EIW +Y +APR PQPAV PVDE+GAL+YAG
Sbjct: 721 EKCSSVLREYHSAVETITDILLEKGEIKADEIWDVYTRAPRIPQPAVSPVDEYGALMYAG 780

Query: 781 RWGIYGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAE 840
           RWGI+G++LPGRVTF+PGN GF+TFGAPRPMETQ+++DETWKL+D IW+KRVQEI+AEA 
Sbjct: 781 RWGIHGISLPGRVTFSPGNVGFSTFGAPRPMETQIISDETWKLVDGIWDKRVQEIKAEA- 840

Query: 841 ASAEVEEDKEKPQLLMASHFL 857
            S E+EEDKEKPQLLMASHFL
Sbjct: 841 -SMEIEEDKEKPQLLMASHFL 852

BLAST of MC01g0705 vs. TAIR 10
Match: AT5G64580.1 (AAA-type ATPase family protein )

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 662/860 (76.98%), Postives = 753/860 (87.56%), Query Frame = 0

Query: 1   MTFMASNAFNFTQLSHPVLPSTTSIGRIQLVSSCSSSSSNHGSAAKIRTRNRIFSFQKLR 60
           MTF  S++   T  S P+ PS T        S  S       + AK+ ++  +F  ++  
Sbjct: 1   MTFYISSSLTPTHFSKPLNPSNTLFPSQFRGSLSSFVRRRKPTEAKLSSKFNLFPSRR-- 60

Query: 61  ELRVSTTRASSSDTDAVVASTNVEEDVESAQLFEKLKGAERQRINKLEELQRKANLQLER 120
                 T  S+S  ++  +S + EED ES +LFEKL+  ER+R++ +EEL+RKAN+QLER
Sbjct: 61  --NGLITCCSTSSFESTESSVSQEEDAESNRLFEKLRETERERLSNMEELERKANVQLER 120

Query: 121 QLVMASSWSRALLTMRGKLKGTEWDPENSHKINFSDFWTLLNSSNVQFIEYSNYGQTMSV 180
           QLVMAS WSR LLTMRGKLKGTEWDPE SH+INFSDF  LL+S++VQ++EYSNYGQT+SV
Sbjct: 121 QLVMASDWSRTLLTMRGKLKGTEWDPETSHRINFSDFMKLLDSNSVQYMEYSNYGQTISV 180

Query: 181 ILPYYKD----GTEGNARNEIIFRRHVIDRMPIDCWNDVWKKLHQQIVNVDVINVDAVPA 240
           ILPYYKD    G E +++ EIIFRRH++DRMPID WNDVWKKLHQQIVNV+V NVD VPA
Sbjct: 181 ILPYYKDGEPLGEEEDSKKEIIFRRHIVDRMPIDGWNDVWKKLHQQIVNVEVFNVDVVPA 240

Query: 241 EVYSSVATAVVWSMRLALSVALYLWIDNLARPIYAKLIPCDLGVPRKITRLPLKRRALGS 300
           EVY++VAT VVWSMRLAL V+LY+WID++ RPIYAKLIPCDLG P K  R PLKR+ALGS
Sbjct: 241 EVYTTVATFVVWSMRLALFVSLYVWIDSITRPIYAKLIPCDLGTPTKKIRQPLKRQALGS 300

Query: 301 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHG 360
           LGKSRAKFISAEE TGVTF DFAGQEYIKRELQEIVRILKNDEEFQ+KGIYCPKGVLLHG
Sbjct: 301 LGKSRAKFISAEEKTGVTFDDFAGQEYIKRELQEIVRILKNDEEFQNKGIYCPKGVLLHG 360

Query: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 420
           PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFAS+RSYAPSIIFIDE
Sbjct: 361 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASSRSYAPSIIFIDE 420

Query: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 480
           IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKV+T+QVLVIGATNRLDILDPALLRK
Sbjct: 421 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVTTSQVLVIGATNRLDILDPALLRK 480

Query: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILN 540
           GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSE+ KE LLQE+AE TEDFTGAELQN+LN
Sbjct: 481 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEDEKEELLQEVAENTEDFTGAELQNVLN 540

Query: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 600
           EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTE+PEELKLRLAYREAAVA+LAC
Sbjct: 541 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEVPEELKLRLAYREAAVAVLAC 600

Query: 601 YFPDTHRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVD 660
           Y PD +RP  ETDI SIRSQPNM Y+ET GRVFARK DYVNSI+RACAPRV+EEEMFG++
Sbjct: 601 YLPDQYRPISETDINSIRSQPNMRYSETSGRVFARKSDYVNSIIRACAPRVVEEEMFGIE 660

Query: 661 NLCWISSKATLEASKLAEFLILQTGMTAFGKAYYRNHGDLVPNLASKLEALREEYLRYAV 720
           NLCWIS+K+TLEAS+ AEFLILQTGMTAFGKAYYRN  DLVPNL  KLEALR+EY+R+AV
Sbjct: 661 NLCWISAKSTLEASQRAEFLILQTGMTAFGKAYYRNQRDLVPNLVPKLEALRDEYMRFAV 720

Query: 721 EKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFPQPAVRPVDEHGALLYAG 780
           EKCSSIL+EY SA+E ITD+LLEKGEI+A+EIW+IY  APR PQ  VRPVDE+GAL+YAG
Sbjct: 721 EKCSSILQEYQSALEEITDVLLEKGEIKADEIWNIYNTAPRIPQKPVRPVDEYGALIYAG 780

Query: 781 RWGIYGVTLPGRVTFAPGNAGFATFGAPRPMETQVVNDETWKLIDDIWEKRVQEIRAEAE 840
           RWGI+GV+LPGRVTF+PGN GFATFGAPRPMETQ+++D+TWKL+D+IW+K+V+EI+  AE
Sbjct: 781 RWGIHGVSLPGRVTFSPGNIGFATFGAPRPMETQIISDDTWKLVDEIWDKKVEEIK--AE 840

Query: 841 ASAEVEEDKEKPQLLMASHF 857
           A  ++EE+K+KPQ+LMA+HF
Sbjct: 841 AVIQIEEEKKKPQILMATHF 854

BLAST of MC01g0705 vs. TAIR 10
Match: AT5G42270.1 (FtsH extracellular protease family )

HSP 1 Score: 287.0 bits (733), Expect = 5.1e-77
Identity = 186/475 (39.16%), Postives = 271/475 (57.05%), Query Frame = 0

Query: 298 GKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGP 357
           G+S++KF    E TGVTF D AG +  K ELQE+V  LKN +++   G   PKG LL GP
Sbjct: 233 GRSKSKFQEVPE-TGVTFGDVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGP 292

Query: 358 PGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEI 417
           PGTGKTLLA+A+AGEAG+PFF+   ++FVE+FVGV ASRV+DLF  A+S AP I+FIDEI
Sbjct: 293 PGTGKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEI 352

Query: 418 DAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKG 477
           DA+G +RG   +GGG  EREQ + Q+LTEMDGF    + V+V+ ATNR D+LD ALLR G
Sbjct: 353 DAVGRQRGA-GMGGGNDEREQTINQLLTEMDGFS-GNSGVIVLAATNRPDVLDSALLRPG 412

Query: 478 RFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNE 537
           RFD+ + V  P   GR+ ILKVH+R K       K+   +++A  T  FTGA+LQN++NE
Sbjct: 413 RFDRQVTVDRPDVAGRVQILKVHSRGKAIG----KDVDYEKVARRTPGFTGADLQNLMNE 472

Query: 538 AGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACY 597
           A IL AR++L  I ++E+ +AL+R        ++ +  + EE K  +AY EA  A++   
Sbjct: 473 AAILAARRELKEISKDEISDALER---IIAGPEKKNAVVSEEKKRLVAYHEAGHALVGAL 532

Query: 598 FPDTHRPFIETDIKSIRSQPN--MHYAETPGR----VFARKIDYVNSIVRACAPRVIEEE 657
            P+ + P  +  I   R Q      +A +  R    +++R     N +  A   RV EE 
Sbjct: 533 MPE-YDPVAKISIIP-RGQAGGLTFFAPSEERLESGLYSRSY-LENQMAVALGGRVAEEV 592

Query: 658 MFGVDNLCWISSKATLEASKLAEFLI------LQTGMTAFGKA--------YYRNHGDLV 717
           +FG +N+   +S   ++ S++A  ++       + G  A G A           +  D  
Sbjct: 593 IFGDENVTTGASNDFMQVSRVARQMVERFGFSKKIGQVAVGGAGGNPFLGQSMSSQKDYS 652

Query: 718 PNLASKLEALREEYLRYAVEKCSSILREYHSAVETITDILLEKGEIRAEEIWHIY 753
              A  ++A   E +  A  +   I+      +  +  +L+EK  +  EE   ++
Sbjct: 653 MATADVVDAEVRELVEKAYVRAKEIITTQIDILHKLAQLLIEKETVDGEEFMSLF 694

BLAST of MC01g0705 vs. TAIR 10
Match: AT1G50250.1 (FTSH protease 1 )

HSP 1 Score: 286.2 bits (731), Expect = 8.8e-77
Identity = 183/475 (38.53%), Postives = 273/475 (57.47%), Query Frame = 0

Query: 298 GKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHGP 357
           G+S++KF    E TGV+F+D AG +  K ELQE+V  LKN +++   G   PKG LL GP
Sbjct: 245 GRSKSKFQEVPE-TGVSFADVAGADQAKLELQEVVDFLKNPDKYTALGAKIPKGCLLVGP 304

Query: 358 PGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDEI 417
           PGTGKTLLA+A+AGEAG+PFF+   ++FVE+FVGV ASRV+DLF  A+S AP I+FIDEI
Sbjct: 305 PGTGKTLLARAVAGEAGVPFFSCAASEFVELFVGVGASRVRDLFEKAKSKAPCIVFIDEI 364

Query: 418 DAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRKG 477
           DA+G +RG   +GGG  EREQ + Q+LTEMDGF    + V+V+ ATNR D+LD ALLR G
Sbjct: 365 DAVGRQRGA-GMGGGNDEREQTINQLLTEMDGFS-GNSGVIVLAATNRPDVLDSALLRPG 424

Query: 478 RFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILNE 537
           RFD+ + V  P   GR+ IL+VH+R K       K+    ++A  T  FTGA+LQN++NE
Sbjct: 425 RFDRQVTVDRPDVAGRVKILQVHSRGKALG----KDVDFDKVARRTPGFTGADLQNLMNE 484

Query: 538 AGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILACY 597
           A IL AR++L  I ++E+ +AL+R        ++ +  + EE K  +AY EA  A++   
Sbjct: 485 AAILAARRELKEISKDEISDALER---IIAGPEKKNAVVSEEKKRLVAYHEAGHALVGAL 544

Query: 598 FPDTHRPFIETDIKSIRSQPN--MHYAETPGR----VFARKIDYVNSIVRACAPRVIEEE 657
            P+ + P  +  I   R Q      +A +  R    +++R     N +  A   RV EE 
Sbjct: 545 MPE-YDPVAKISIIP-RGQAGGLTFFAPSEERLESGLYSRSY-LENQMAVALGGRVAEEV 604

Query: 658 MFGVDNLCWISSKATLEASKLAEFLILQTGMT--------------AFGKAYYRNHGDLV 717
           +FG +N+   +S   ++ S++A  +I + G +               F      +  D  
Sbjct: 605 IFGDENVTTGASNDFMQVSRVARQMIERFGFSKKIGQVAVGGPGGNPFMGQQMSSQKDYS 664

Query: 718 PNLASKLEALREEYLRYAVEKCSSILREYHSAVETITDILLEKGEIRAEEIWHIY 753
              A  ++A   E +  A ++ + I+  +   +  +  +L+EK  +  EE   ++
Sbjct: 665 MATADIVDAEVRELVEKAYKRATEIITTHIDILHKLAQLLIEKETVDGEEFMSLF 706

BLAST of MC01g0705 vs. TAIR 10
Match: AT5G15250.1 (FTSH protease 6 )

HSP 1 Score: 283.5 bits (724), Expect = 5.7e-76
Identity = 206/555 (37.12%), Postives = 291/555 (52.43%), Query Frame = 0

Query: 209 IDCWNDVWKKLHQQIVNVDVINVDAVPAEVYSSVATAVVWSMRLALSVALYLWIDNLARP 268
           ++  N V  K+ +  VN+  + VD V  E+           M +     L  ++ NL  P
Sbjct: 122 VEISNPVVGKIQRVRVNLPGLPVDLV-REMKEKNVDFAAHPMNVNWGAFLLNFLGNLGFP 181

Query: 269 IYAKLIPCDLGVPRKITRLPLKRRALGSLGKSRAKFISAEETTGVTFSDFAGQEYIKREL 328
           +   ++   L +     R P        LG+S+AKF   E  TG+TF D AG +  K++ 
Sbjct: 182 L---ILLVSLLLTSSSRRNPAGPNLPFGLGRSKAKF-QMEPNTGITFEDVAGVDEAKQDF 241

Query: 329 QEIVRILKNDEEFQDKGIYCPKGVLLHGPPGTGKTLLAKAIAGEAGLPFFAANGTDFVEM 388
           +EIV  LK  E+F   G   PKGVLL GPPGTGKTLLAKAIAGEAG+PFF+ +G++F+EM
Sbjct: 242 EEIVEFLKTPEKFSALGAKIPKGVLLTGPPGTGKTLLAKAIAGEAGVPFFSLSGSEFIEM 301

Query: 389 FVGVAASRVKDLFASARSYAPSIIFIDEIDAIGSKRGGPDIGGGGAEREQGLLQILTEMD 448
           FVGV ASR +DLF  A++ +P I+FIDEIDA+G  R G  IGGG  EREQ L QILTEMD
Sbjct: 302 FVGVGASRARDLFNKAKANSPCIVFIDEIDAVGRMR-GTGIGGGNDEREQTLNQILTEMD 361

Query: 449 GFKVSTAQVLVIGATNRLDILDPALLRKGRFDKIIRVGLPSKDGRLAILKVHARNKFFRS 508
           GF  +T  V+VI ATNR +ILD ALLR GRFD+ + VGLP   GR  ILKVH+R+K    
Sbjct: 362 GFAGNTG-VIVIAATNRPEILDSALLRPGRFDRQVSVGLPDIRGREEILKVHSRSK---- 421

Query: 509 EEIKEALLQEIAELTEDFTGAELQNILNEAGILTARKDLDYIGREELLEALKRQKGTFET 568
           +  K+  L  IA  T  F+GA+L N++NEA IL  R+  D I   E+ +++ R       
Sbjct: 422 KLDKDVSLSVIAMRTPGFSGADLANLMNEAAILAGRRGKDKITLTEIDDSIDR----IVA 481

Query: 569 GQEDSTEIPEELKLRLAYREAAVAILACYFPDTHRPFIETDIKSIRSQPNMHYAETPGR- 628
           G E +  I  + K  +AY E   AI A    + H P  +  +   R Q        PG  
Sbjct: 482 GMEGTKMIDGKSKAIVAYHEVGHAICAT-LTEGHDPVQKVTLVP-RGQARGLTWFLPGED 541

Query: 629 -VFARKIDYVNSIVRACAPRVIEEEMFGVDNLCWISSKATLEASKLAEFLILQTGMTAFG 688
                K      IV     R  E+ +FG   +   ++    + +++A  ++   GM+  G
Sbjct: 542 PTLVSKQQLFARIVGGLGGRAAEDVIFGEPEITTGAAGDLQQVTEIARQMVTMFGMSEIG 601

Query: 689 ------KAYYRN--------HGDLVPNLASKLEALREEYLRYAVEKCSSILREYHSAVET 748
                  A  +N           +   LA  +++  ++ +  A E     +R    A++ 
Sbjct: 602 PWALTDPAVKQNDVVLRMLARNSMSEKLAEDIDSCVKKIIGDAYEVAKKHVRNNREAIDK 659

BLAST of MC01g0705 vs. TAIR 10
Match: AT1G06430.1 (FTSH protease 8 )

HSP 1 Score: 282.3 bits (721), Expect = 1.3e-75
Identity = 189/478 (39.54%), Postives = 256/478 (53.56%), Query Frame = 0

Query: 297 LGKSRAKFISAEETTGVTFSDFAGQEYIKRELQEIVRILKNDEEFQDKGIYCPKGVLLHG 356
           +G+S+AKF   E  TGVTF D AG +  K++  E+V  LK  E F   G   PKGVLL G
Sbjct: 202 IGQSKAKF-QMEPNTGVTFDDVAGVDEAKQDFMEVVEFLKKPERFTAVGARIPKGVLLVG 261

Query: 357 PPGTGKTLLAKAIAGEAGLPFFAANGTDFVEMFVGVAASRVKDLFASARSYAPSIIFIDE 416
           PPGTGKTLLAKAIAGEAG+PFF+ +G++FVEMFVGV ASRV+DLF  A+  AP I+F+DE
Sbjct: 262 PPGTGKTLLAKAIAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFKKAKENAPCIVFVDE 321

Query: 417 IDAIGSKRGGPDIGGGGAEREQGLLQILTEMDGFKVSTAQVLVIGATNRLDILDPALLRK 476
           IDA+G +R G  IGGG  EREQ L Q+LTEMDGF+ +T  V+V+ ATNR DILD ALLR 
Sbjct: 322 IDAVGRQR-GTGIGGGNDEREQTLNQLLTEMDGFEGNTG-VIVVAATNRADILDSALLRP 381

Query: 477 GRFDKIIRVGLPSKDGRLAILKVHARNKFFRSEEIKEALLQEIAELTEDFTGAELQNILN 536
           GRFD+ + V +P   GR  ILKVH+ NK F S       L+ IA  T  F+GA+L N+LN
Sbjct: 382 GRFDRQVSVDVPDVKGRTDILKVHSGNKKFES----GVSLEVIAMRTPGFSGADLANLLN 441

Query: 537 EAGILTARKDLDYIGREELLEALKRQKGTFETGQEDSTEIPEELKLRLAYREAAVAILAC 596
           EA IL  R+    I  +E+ +++ R       G E +     + K  +AY E   AI   
Sbjct: 442 EAAILAGRRGKTAISSKEIDDSIDR----IVAGMEGTVMTDGKSKSLVAYHEVGHAICGT 501

Query: 597 YFPDTHRPFIETDIKSIRSQPNMHYAETPGRVFARKIDYVNSIVRACAPRVIEEEMFGVD 656
             P        T I   +++    +  +       K      IV     R  EE +FG  
Sbjct: 502 LTPGHDAVQKVTLIPRGQARGLTWFIPSDDPTLISKQQLFARIVGGLGGRAAEEVIFGES 561

Query: 657 NLCWISSKATLEASKLAEFLILQTGMTAFG---------------KAYYRNHGDLVPNLA 716
            +   +     + + LA+ ++   GM+  G               +   RN   +   LA
Sbjct: 562 EVTTGAVSDLQQITGLAKQMVTTFGMSEIGPWSLMDSSEQSDVIMRMMARN--SMSEKLA 621

Query: 717 SKLEALREEYLRYAVEKCSSILREYHSAVETITDILLEKGEIRAEEIWHIYEKAPRFP 760
           + ++   +     A E   S +R    A++ I +ILLEK  +  +E   I  +    P
Sbjct: 622 NDIDTAVKTLSDKAYEIALSQIRNNREAMDKIVEILLEKETMSGDEFRAILSEFTEIP 666