MC01g0696 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0696
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial
Location	MC01: 13103485 .. 13107625 (+)
RNA-Seq Expression	MC01g0696
Synteny	MC01g0696

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAGACGTGAAGAACATGAACACCTTGAACAGATATAAACGCCAACGTTGCAGACTAGATAAGGTAAAAGATGGATTATATTATTTGTTTAATTGAATTGTAGATTGAAGAAAAAAACTGGAAGCCCCGTTATCTCTTCCCTTTTGTTTCTATTGCAATCTCCATTGGAAATCATCAGTCCGAACATCCTCTCGGTCCGGCCATGGCTTTTCAAGCGAGAAAAGCAACGAAGATCATCCCTCACTTTGGAAACAGGATGATGAATTTCTTGGGTTCGATTGAAATGGGTTTCGCGAGCTCTTATTATGCAACCCAGCTTCACAAAACTCCATTTTCGGTTCAAAAGATCGGGAATGGAGTCGTGGGTTTCGAAGATATGCCTACCCATTTATCTGCTCGTCGTTTCAAATCGATTAAAGTGGAAAAACGATTGGATTCTGTCAACGATGATCGTGACAATCAGGTAGACTACATTCTGGTGCTGTCAACTTTGATTCCATCTTCCAATTTTATAATCCCAAATCATTTTTAGATTGTTGTTGCTCCTGTCATGCGTTGAAATGGCTCGTAATTTTTTAAACGTTATTCGAAAACTGTGTTCTCGGAATCGGCATCTAAAAAGTTCCTTTCCCCTAAAAGAAAAGTCATACGTTGACAAGTAACTCTAGACTTTGAACTGTACTTTTTATTTTCCATTCTGCTTGTCATCCCCGCTCCCCATTATTCTATCACAGAACAGATCCAGTGGGGACGGGGTGGGACCTGTAGCTCAGAGGATTAGAACATGTGGCTACGAACCAGAATGTCGGGGGTTTGATTCTTGATCAGAAAGCGGTATGCTTGGGAAGACCATTTCTGGTCGAGACGTGTTAATGAAATCAATCCGTGAAATAAGGTCTCGTTCATTCTTGGGGTGAATAAATAGAGACTACTTGGAAATTCTTTTTCTTTTCTTTTCCTCTTTATCTTGCCGAGATGAATCTGAATGGTTGGTGATCGTGAAGAATGTTGCTAGTCAGAGAAGAAAATGAGCTTGAGAGCAAAAAGGAGACCTTATGTAACTTGTAATTTGATTGGTTTGATGGGTATTTTAGCTTCAATAGAGGCCATTTTTGCTTAAAATTTTCAGGTCAGGTTATTCCTTAATCTTTGTTTATTAAGGTGCCCTAAGTCACAAGTCAATATGGTATTTCCATTTCCGTATTCTGGAAAAGTTGTAATTTAATTTTTTTAATCATCTAGTATAATATATGTTCAAGTTCAATAGTCAAAGTTCTGGGGTCAAAGTGCCCATTCATATAGAGATTTCTTCCAAAGGCTCATCGTTTGTGTACAAGTTGATGATATTTGATGTCAAGTGCTGAAAGTGAAACTTGCATTTATGGTACAAGGTTTTGGAGTTTCCAGGAGGAAAGGTTAGATTTACTCCTCACATGAGTTTCATCCCTGATCGCGACAAGGAGCGGATACCCTGCTTTCGAGTTCTCGATGACAACGGTCAGGCTAGTATATTCAGTAATTTTACACAGGTAATTCTATGCTGAACATGTCCTGTGATGTCTCTCTCTCTCTTTCTTGTCCCAACTTCAAGTGCAAAGGAAGGAACTTACGAACTTGATACTACTTTTCATTTAGGTGAGCAAGGAGGTTGCTGTAAAGATGTACAATGACATGGTTACTCTCCAAACAATGGACACTATCTTATATGAAGCGCAAAGGCAGGGAAGAATTTCCTTTTATCTGACCTCCATTGGAGAAGAGGCAATCAACATTGCTTCTGCAGCAGCACTGTCATTGGATGACCTTATTGTACCACAGGTTACAATTGCATATCTCCCCTTCATCATCCTCCTTAGTAATATGAAGTTAATTCTTATCCTCAAAGACAAATGTGAACAAAGACAAAGGTGCAACATGCTCCCGGGGGCAACCTCGGATGCATAAGATGTTTACGATATGTGAAGTGGTGGCTTGCGACCTGATACCAGGGATATTAACAGGAAAAAATAGACAAATGCGCAATACTGTTCCCTTGAATTAACATCTTACCTAATAGTTGACATGAATGAGATTGATTTGTAAGTCTAATTCTCCCATGTCATGCAGTACAGGGAAGCAGGAGTACTTATATGGCGTGGTTTCACTTTGAAAGAATTTGCAAGCCAGTGCTTCGGAAACAAAGATGATCATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCCAAAAGATACAATTACTTTACTGTAGCATCAACAATCGCGTAATATTCGAGCTCAATATGTCAAAACTTTTTAGTAGATCTTCTAACACTTCCAATTTTATTCCTTATATTTTACTTTTACTTATAGTTCTCAAATCCCTCATGCCGTTGGTGCTGCATATTCAATGAAGATGGAAAAGAAAGATGCTTGTGCAGTCACTTATTTTGGTGATGGAGGAACAAGTGAGGTAAATAATCCTAAAAAAATTTTAGTGTTAGAACTGTTCCATCTGCTGTTACCTCAGAGAGAAGACTTTTGAGGGGATGTGCTATTCAAGTTGAAAATCAGCCACACTCACGACCTTCCTTGAATAGGATATGTTCTATAGGTTGTACTACTGTATTATTAAGTTGTAAATCAGTCCATCAAATTTGGTTGATACTTATGACTGCAGAGTCTTCCGAGTTTTCGGGTTGATTTTTCTATTGTCTATCTGATATATCATGTTATATTAGGGAGATTTCCACGCGGCTTTAAATTTTGCAGCGGTTTTGGAGGCGCCTGTTATTTTTATTTGTCGGAACAATGGATGGGCAATCAGTACTCCTATTTCAGACCAGTTTCGGAGTATCTCATTCTTCTCTGAATCTGCAGTTGTAAAGAATGTACCATGAAATCTAGAGTTTATTGTTTTGTCATTTTGCAGGTGATGGTGTGATTGTGAAGGGTCGGGCTTATGGAGTTCGTAGTATCCGTGTCGATGGTAATGATGCACTTGCTGTATACAATGCAGTTCGAGCTGCTCGAGAAATGGCAATTCAAGAACAAAGGCCAATATTAATTGAGGTAATAACAATTTCACTATTGCACTCTTGAAATGCACTTTGAAGATCTGATGCCTTTTTGTCAGCAGGCTCTCACATACAGAGTTGGACACCACACGACATCAGACGACTCAACCAGGTATCGTCCCGCTGAGGAAATCGAATGGTGGAGGGTAGCAAAGGACCCTGTATCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATGAGGCAGAATCTGAGCTTAGAAGTAGCCTAAGAAAACAGGTAAAGTTTATTTTCAGAATAAGATATTTCCAGAGAACAAGATAGTTTTCAACTCAAATTTATAATTTGCCACATCAAATGAATTGGGTTAAAAATAACACATTAAACTAGGTAAACAAGAACAAGATAGTTTTCAACTCAAATTTATAATTTGCTGCTCTATTAGTCTTCTTCACCAGAAACCCCAACCTTTATATATTTAGCAGATCCAAAATTGAATACTACAAAATGATTGGACATTTGGACAGAATCAGGTGAAAGTGAAACTTTTTTACTTTTTCAAAAAATTCTTTTGGATTTTTAGTCAACTTTAAAAAGTAAAAACATATTCTTGAAAGTTACTTCTCTAAAATGCTCTTACCGGGTAGATTACAAAACGAAGAAACATATTTCCAGAAACCAAAAATAAGATGGTTATCAAAACTATTAACTACTGTGTACAGTTCCTAATTCTTCATGTTTTTGGTTCCTACAGCTATTAGAAGAAATTCAGTTGGCAGAGAGGCGTGAGAAGCCCCCAGTGGCAGAGGCTTTCACAGATGTGTATGATGTTCCTCCCTCCAACCTCAAAGAGCAAGAGAGTTGGTTAAGAAAAACAATCAAAACACACCCGCAAGATTACCCATCTAATTTTCCTCTGTAACCTGACTAGGTTTTGGAACTCTGCCTTAAAAATTAGGTTTGCTTTCCCCAACCTGTGGTTAAAGCAGTTAGAGAGAGGTGAGAATGGTGGCAGTGTTTGCAAATAAAGGACAAATAAGGCATCCTGTCTAAATAAAGTTATCCTTCTGCGAAGAATGTCATATTCAATATACAAACTGATAAAAGAAACCACCA

mRNA sequence

CCAGACGTGAAGAACATGAACACCTTGAACAGATATAAACGCCAACGTTGCAGACTAGATAAGTCCGAACATCCTCTCGGTCCGGCCATGGCTTTTCAAGCGAGAAAAGCAACGAAGATCATCCCTCACTTTGGAAACAGGATGATGAATTTCTTGGGTTCGATTGAAATGGGTTTCGCGAGCTCTTATTATGCAACCCAGCTTCACAAAACTCCATTTTCGGTTCAAAAGATCGGGAATGGAGTCGTGGGTTTCGAAGATATGCCTACCCATTTATCTGCTCGTCGTTTCAAATCGATTAAAGTGGAAAAACGATTGGATTCTGTCAACGATGATCGTGACAATCAGGTTTTGGAGTTTCCAGGAGGAAAGGTTAGATTTACTCCTCACATGAGTTTCATCCCTGATCGCGACAAGGAGCGGATACCCTGCTTTCGAGTTCTCGATGACAACGGTCAGGCTAGTATATTCAGTAATTTTACACAGGTGAGCAAGGAGGTTGCTGTAAAGATGTACAATGACATGGTTACTCTCCAAACAATGGACACTATCTTATATGAAGCGCAAAGGCAGGGAAGAATTTCCTTTTATCTGACCTCCATTGGAGAAGAGGCAATCAACATTGCTTCTGCAGCAGCACTGTCATTGGATGACCTTATTGTACCACAGTACAGGGAAGCAGGAGTACTTATATGGCGTGGTTTCACTTTGAAAGAATTTGCAAGCCAGTGCTTCGGAAACAAAGATGATCATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCCAAAAGATACAATTACTTTACTGTAGCATCAACAATCGCTTCTCAAATCCCTCATGCCGTTGGTGCTGCATATTCAATGAAGATGGAAAAGAAAGATGCTTGTGCAGTCACTTATTTTGGTGATGGAGGAACAAGTGAGGGAGATTTCCACGCGGCTTTAAATTTTGCAGCGGTTTTGGAGGCGCCTGTTATTTTTATTTGTCGGAACAATGGATGGGCAATCAGTACTCCTATTTCAGACCAGTTTCGGAGTGATGGTGTGATTGTGAAGGGTCGGGCTTATGGAGTTCGTAGTATCCGTGTCGATGGTAATGATGCACTTGCTGTATACAATGCAGTTCGAGCTGCTCGAGAAATGGCAATTCAAGAACAAAGGCCAATATTAATTGAGGCTCTCACATACAGAGTTGGACACCACACGACATCAGACGACTCAACCAGGTATCGTCCCGCTGAGGAAATCGAATGGTGGAGGGTAGCAAAGGACCCTGTATCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATGAGGCAGAATCTGAGCTTAGAAGTAGCCTAAGAAAACAGCTATTAGAAGAAATTCAGTTGGCAGAGAGGCGTGAGAAGCCCCCAGTGGCAGAGGCTTTCACAGATGTGTATGATGTTCCTCCCTCCAACCTCAAAGAGCAAGAGAGTTGGTTAAGAAAAACAATCAAAACACACCCGCAAGATTACCCATCTAATTTTCCTCTGTAACCTGACTAGGTTTTGGAACTCTGCCTTAAAAATTAGGTTTGCTTTCCCCAACCTGTGGTTAAAGCAGTTAGAGAGAGGTGAGAATGGTGGCAGTGTTTGCAAATAAAGGACAAATAAGGCATCCTGTCTAAATAAAGTTATCCTTCTGCGAAGAATGTCATATTCAATATACAAACTGATAAAAGAAACCACCA

Coding sequence (CDS)

ATGAACACCTTGAACAGATATAAACGCCAACGTTGCAGACTAGATAAGTCCGAACATCCTCTCGGTCCGGCCATGGCTTTTCAAGCGAGAAAAGCAACGAAGATCATCCCTCACTTTGGAAACAGGATGATGAATTTCTTGGGTTCGATTGAAATGGGTTTCGCGAGCTCTTATTATGCAACCCAGCTTCACAAAACTCCATTTTCGGTTCAAAAGATCGGGAATGGAGTCGTGGGTTTCGAAGATATGCCTACCCATTTATCTGCTCGTCGTTTCAAATCGATTAAAGTGGAAAAACGATTGGATTCTGTCAACGATGATCGTGACAATCAGGTTTTGGAGTTTCCAGGAGGAAAGGTTAGATTTACTCCTCACATGAGTTTCATCCCTGATCGCGACAAGGAGCGGATACCCTGCTTTCGAGTTCTCGATGACAACGGTCAGGCTAGTATATTCAGTAATTTTACACAGGTGAGCAAGGAGGTTGCTGTAAAGATGTACAATGACATGGTTACTCTCCAAACAATGGACACTATCTTATATGAAGCGCAAAGGCAGGGAAGAATTTCCTTTTATCTGACCTCCATTGGAGAAGAGGCAATCAACATTGCTTCTGCAGCAGCACTGTCATTGGATGACCTTATTGTACCACAGTACAGGGAAGCAGGAGTACTTATATGGCGTGGTTTCACTTTGAAAGAATTTGCAAGCCAGTGCTTCGGAAACAAAGATGATCATTGTAAAGGGAGGCAGATGCCTGTCCATTATGGGTCCAAAAGATACAATTACTTTACTGTAGCATCAACAATCGCTTCTCAAATCCCTCATGCCGTTGGTGCTGCATATTCAATGAAGATGGAAAAGAAAGATGCTTGTGCAGTCACTTATTTTGGTGATGGAGGAACAAGTGAGGGAGATTTCCACGCGGCTTTAAATTTTGCAGCGGTTTTGGAGGCGCCTGTTATTTTTATTTGTCGGAACAATGGATGGGCAATCAGTACTCCTATTTCAGACCAGTTTCGGAGTGATGGTGTGATTGTGAAGGGTCGGGCTTATGGAGTTCGTAGTATCCGTGTCGATGGTAATGATGCACTTGCTGTATACAATGCAGTTCGAGCTGCTCGAGAAATGGCAATTCAAGAACAAAGGCCAATATTAATTGAGGCTCTCACATACAGAGTTGGACACCACACGACATCAGACGACTCAACCAGGTATCGTCCCGCTGAGGAAATCGAATGGTGGAGGGTAGCAAAGGACCCTGTATCTAGATTTAGGAAATGGATTGAAAGCAATGGTTGGTGGAGTAATGAGGCAGAATCTGAGCTTAGAAGTAGCCTAAGAAAACAGCTATTAGAAGAAATTCAGTTGGCAGAGAGGCGTGAGAAGCCCCCAGTGGCAGAGGCTTTCACAGATGTGTATGATGTTCCTCCCTCCAACCTCAAAGAGCAAGAGAGTTGGTTAAGAAAAACAATCAAAACACACCCGCAAGATTACCCATCTAATTTTCCTCTGTAA

Protein sequence

MNTLNRYKRQRCRLDKSEHPLGPAMAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMPTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFPL

Homology

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BLAST of MC01g0696 vs. ExPASy Swiss-Prot
Match: Q84JL2 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g09300 PE=1 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 1.1e-180
Identity = 297/397 (74.81%), Postives = 353/397 (88.92%), Query Frame = 0


Query: 109 DNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNGQASIFSNFTQVSKEVAVKMYN 168
           ++QV++FPGGKV FTP + FI + DKER+PC+RVLDDNGQ    S F QVS+EVAVK+Y+
Sbjct: 76  NHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 135

Query: 169 DMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAAALSLDDLIVPQYREAGVLIWR 228
           DMVTLQ MD I YEAQRQGR+SFY T+IGEEAINIASAAAL+  D+I PQYRE GVL+WR
Sbjct: 136 DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 195

Query: 229 GFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVASTIASQIPHAVGAAYSMKMEK 288
           GFTL+EFA+QCFGNK D+ KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KM+K
Sbjct: 196 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 255

Query: 289 KDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVIVK 348
           KDACAVTYFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGV+VK
Sbjct: 256 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 315

Query: 349 GRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRVGHHTTSDDSTRYRP 408
           GRAYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYRVGHH+TSDDSTRYR 
Sbjct: 316 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 375

Query: 409 AEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLRKQLLEEIQLAERREKPPVAE 468
           A EIEWW  A++P+SRFR WIESNGWWS++ ES+LRS ++K++LE +++AE+ EKP +  
Sbjct: 376 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 435

Query: 469 AFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFPL 506
            F+DVYDVPPSNL+EQE  +R+TI +HPQDYPS+ PL
Sbjct: 436 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of MC01g0696 vs. ExPASy Swiss-Prot
Match: Q9LPL5 (2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g21400 PE=2 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 3.6e-168
Identity = 279/416 (67.07%), Postives = 345/416 (82.93%), Query Frame = 0


Query: 91  RFKSIKVEKRLDSV---NDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNG 150
           R +S  VE + D +    D+ D Q L+FPGGKV +T  M FIP+    RIPC+RVLD++G
Sbjct: 55  RHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDG 114

Query: 151 QASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAA 210
           +    S+F  VS+++AV+MY  M TLQ MD I YEAQRQGRISFYLTS+GEEAINIASAA
Sbjct: 115 RIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAA 174

Query: 211 ALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVA 270
           ALS DD+++PQYRE GVL+WRGFTL+EFA+QCFGNK D+ KGRQMP+HYGS R NYFT++
Sbjct: 175 ALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTIS 234

Query: 271 STIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRN 330
           S IA+Q+P A G  YS+KM+KK+AC VT+ GDGGTSEGDFHA LNFAAV+EAPV+FICRN
Sbjct: 235 SPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRN 294

Query: 331 NGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILI 390
           NGWAIST IS+QFRSDG++VKG+AYG+RSIRVDGNDALAVY+AVR+AREMA+ EQRP+LI
Sbjct: 295 NGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLI 354

Query: 391 EALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSL 450
           E +TYRVGHH+TSDDST+YR A+EI++W+++++PV+RFRKW+E NGWWS E ES+LRS+ 
Sbjct: 355 EMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNA 414

Query: 451 RKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RKQLL+ IQ AE+ EK P+ E F DVYDV P NL+EQE  L++ +K  PQDYP  F
Sbjct: 415 RKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGF 470

[Close]

BLAST of MC01g0696 vs. ExPASy Swiss-Prot
Match: Q54M22 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=bkdA PE=3 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.7e-113
Identity = 191/370 (51.62%), Postives = 258/370 (69.73%), Query Frame = 0


Query: 134 KERIPCFRVLDDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYL 193
           K  IPC+ ++D  G  S        SKE  +KMY  M+TL  MD+ILY+ QRQGRISFY+
Sbjct: 61  KHYIPCYTIMDQEGVVSKPDQDPNFSKEEVIKMYTTMLTLNVMDSILYDVQRQGRISFYM 120

Query: 194 TSIGEEAINIASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMP 253
           TS GEEAI+I SAAAL + D I  QYRE GV +WRGFT+ +  +QC  N+ D  KGRQMP
Sbjct: 121 TSFGEEAIHIGSAAALEMSDTIFAQYRETGVFMWRGFTINDIINQCCTNEHDLGKGRQMP 180

Query: 254 VHYGSKRYNYFTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNF 313
           +H+GS++ N  T++S + +Q+P AVG++Y+ K+  +  C + YFG+G  SEGDFHAA+NF
Sbjct: 181 MHFGSRKINLQTISSPLTTQLPQAVGSSYAQKLAGEKNCTIVYFGEGAASEGDFHAAMNF 240

Query: 314 AAVLEAPVIFICRNNGWAISTPISDQFRSDGVIVKG-RAYGVRSIRVDGNDALAVYNAVR 373
           AA L  P IF CRNN WAISTP  +Q++ DG+  +G   YG+++IRVDGND  AVYN  +
Sbjct: 241 AAALSTPTIFFCRNNKWAISTPSKEQYKGDGIAGRGPNGYGMKTIRVDGNDIWAVYNVTK 300

Query: 374 AAREMAIQEQRPILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESN 433
            AR++A++EQ P+LIEA+TYRVGHH+TSDDS+RYR  EEI  W+  K+P+SR R ++   
Sbjct: 301 LARKIAVEEQVPVLIEAMTYRVGHHSTSDDSSRYRTVEEINAWKEGKNPISRLRNYMNHK 360

Query: 434 GWWSNEAESELRSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTI 493
           GWWS+  E E  ++ R  + E +  AE++ KP + E FTDVYD P  NL EQ+  L + +
Sbjct: 361 GWWSDAQEKETIANARTTVRESLVNAEKQYKPSINEIFTDVYDKPTPNLIEQQKELIEHL 420

Query: 494 KTHPQDYPSN 503
           K +P +YP N
Sbjct: 421 KLYPDEYPLN 430

[Close]

BLAST of MC01g0696 vs. ExPASy Swiss-Prot
Match: P50136 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=10090 GN=Bckdha PE=1 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 9.1e-111
Identity = 194/412 (47.09%), Postives = 268/412 (65.05%), Query Frame = 0


Query: 89  ARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNGQ 148
           AR   S + +++  S++D       +FPG    F   + FI       IP +RV+D  GQ
Sbjct: 30  ARSHPSRQQQQQFPSLDDKP-----QFPGASAEFVDKLEFIQPNVISGIPIYRVMDRQGQ 89

Query: 149 ASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAAA 208
               S    + +E  +K Y  M  L TMD ILYE+QR+GRISFY+T+ GEE  ++ SAAA
Sbjct: 90  IINPSEDPHLPQEEVLKFYRSMTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAA 149

Query: 209 LSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVAS 268
           L   DL+  QYREAGVL++R + L+ F SQC+GN +D  KGRQMPVHYG K  ++ T++S
Sbjct: 150 LERTDLVFGQYREAGVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISS 209

Query: 269 TIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNN 328
            +A+QIP AVGAAY+ K    +   + YFG+G  SEGD HA  NFAA LE P+IF CRNN
Sbjct: 210 PLATQIPQAVGAAYAAKRANANRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNN 269

Query: 329 GWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIE 388
           G+AISTP S+Q+R DG+  +G  YG++SIRVDGND  AVYNA + AR  A+ E +P LIE
Sbjct: 270 GYAISTPTSEQYRGDGIAARGPGYGIKSIRVDGNDVFAVYNATKEARRRAVAENQPFLIE 329

Query: 389 ALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLR 448
           A+TYR+GHH+TSDDS+ YR  +E+ +W     P+SR R+++ + GWW  E E   R   R
Sbjct: 330 AMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRQYLLNQGWWDEEQEKAWRKQSR 389

Query: 449 KQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYP 501
           K+++E  + AER+ KP  +  F+DVY   P+ L+ Q+  L + ++T+ + YP
Sbjct: 390 KKVMEAFEQAERKLKPNPSLLFSDVYQEMPAQLRRQQESLARHLQTYGEHYP 436

[Close]

BLAST of MC01g0696 vs. ExPASy Swiss-Prot
Match: A5A6H9 (2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes OX=9598 GN=BCKDHA PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 1.5e-110
Identity = 192/387 (49.61%), Postives = 255/387 (65.89%), Query Frame = 0


Query: 114 EFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNGQASIFSNFTQVSKEVAVKMYNDMVTL 173
           +FPG    F   + FI       IP +RV+D  GQ    S    + KE  +K+Y  M  L
Sbjct: 53  QFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLL 112

Query: 174 QTMDTILYEAQRQGRISFYLTSIGEEAINIASAAALSLDDLIVPQYREAGVLIWRGFTLK 233
            TMD ILYE+QRQGRISFY+T+ GEE  ++ SAAAL   DL+  QYREAGVL++R + L+
Sbjct: 113 NTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLE 172

Query: 234 EFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVASTIASQIPHAVGAAYSMKMEKKDACA 293
            F +QC+GN  D  KGRQMPVHYG K  ++ T++S +A+QIP AVGAAY+ K    +   
Sbjct: 173 LFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVV 232

Query: 294 VTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVIVKGRAYG 353
           + YFG+G  SEGD HA  NFAA LE P+IF CRNNG+AISTP S+Q+R DG+  +G  YG
Sbjct: 233 ICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYG 292

Query: 354 VRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRVGHHTTSDDSTRYRPAEEIE 413
           + SIRVDGND  AVYNA + AR  A+ E +P LIEA+TYR+GHH+TSDDS+ YR  +E+ 
Sbjct: 293 IMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVN 352

Query: 414 WWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLRKQLLEEIQLAERREKPPVAEAFTDV 473
           +W     PVSR R ++ S GWW  E E   R   RK+++E  + AER+ KP     F+DV
Sbjct: 353 YWDKQDHPVSRLRHYLLSQGWWDEEQEKAWRKQSRKKVMEAFEQAERKPKPNPNLLFSDV 412

Query: 474 YDVPPSNLKEQESWLRKTIKTHPQDYP 501
           Y   P+ L++Q+  L + ++T+ + YP
Sbjct: 413 YQEMPAQLRKQQESLARHLQTYGEHYP 439

[Close]

BLAST of MC01g0696 vs. NCBI nr
Match: XP_022132554.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Momordica charantia])

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 25  MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 84
           MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP
Sbjct: 1   MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 60

Query: 85  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 144
           THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD
Sbjct: 61  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 120

Query: 145 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 204
           DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA
Sbjct: 121 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 180

Query: 205 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 264
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 240

Query: 265 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 324
           TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 325 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 384
           CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 385 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 444
           ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 420

Query: 445 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 504
           SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP
Sbjct: 421 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 480

[Close]

BLAST of MC01g0696 vs. NCBI nr
Match: XP_008441060.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucumis melo] >KAA0025521.1 2-oxoisovalerate dehydrogenase subunit alpha 2 [Cucumis melo var. makuwa] >TYK25677.1 2-oxoisovalerate dehydrogenase subunit alpha 2 [Cucumis melo var. makuwa])

HSP 1 Score: 855 bits (2208), Expect = 1.34e-310
Identity = 420/482 (87.14%), Postives = 451/482 (93.57%), Query Frame = 0


Query: 25  MAFQARKAT-KIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 84
           MAFQ RKAT KIIPHF N M  FLG +EMGF +SY +T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSY-STHLRNTSFSAHKIGTRILGSENM 60

Query: 85  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 144
           PTHL +RRF+SIKV K+LD VNDDRDNQVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120

Query: 145 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 204
           DDNGQ S++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180

Query: 205 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 264
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240

Query: 265 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 324
           FTVASTIASQIPHAVGAAYSMKM+KKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 325 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 384
           ICRNNGWAISTP+SDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 385 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 444
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETEL 420

Query: 445 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RSSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480

[Close]

BLAST of MC01g0696 vs. NCBI nr
Match: XP_023543630.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846 bits (2185), Expect = 2.62e-307
Identity = 415/481 (86.28%), Postives = 445/481 (92.52%), Query Frame = 0


Query: 25  MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 84
           MAF  RKATKIIPHF N M  FLGS             L +TPF+V KIGN V+GFE+M 
Sbjct: 1   MAFHVRKATKIIPHFRNGM-RFLGS-------------LRETPFAVHKIGNKVLGFENML 60

Query: 85  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 144
            HLSA RFKS KVEK++DSVNDDRDNQVL FPGGKV+FTP +SF+P+R++ER+PCFRVLD
Sbjct: 61  NHLSAHRFKSTKVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLD 120

Query: 145 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 204
           DNGQ S+FSNFTQVSKE+A+KMYNDMVTLQTMDTI YEAQRQGRISFY+TS+GEEAINIA
Sbjct: 121 DNGQPSMFSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIA 180

Query: 205 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 264
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 265 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 324
           TVASTIASQIPHAVGAAYSMKMEK+DACAVTYFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300

Query: 325 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 384
           CRNNGWAISTPISDQFRSDGV+VKGRAYGVRSIRVDGNDALAVYN VRAAR+MAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQEQRP 360

Query: 385 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 444
           ILIEALTYRVGHHTTSDDSTRYRP EE+EWWRVAKDPVSRFRKWIESNGWWS+EAE+ELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420

Query: 445 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 504
           SSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPS+FP
Sbjct: 421 SSLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFP 467

[Close]

BLAST of MC01g0696 vs. NCBI nr
Match: XP_004148662.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial isoform X1 [Cucumis sativus] >KGN48345.1 hypothetical protein Csa_003053 [Cucumis sativus])

HSP 1 Score: 846 bits (2186), Expect = 3.01e-307
Identity = 416/482 (86.31%), Postives = 450/482 (93.36%), Query Frame = 0


Query: 25  MAFQARKAT-KIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 84
           MAFQ RKAT KIIPHF N M  FLG +EMGF +SY +T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSY-STHLRNTSFSAHKIGTRILGSENM 60

Query: 85  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 144
           PTHL +RRF+SIKV K+L+ VNDDRD+QVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120

Query: 145 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 204
           DDNG+  ++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180

Query: 205 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 264
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240

Query: 265 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 324
           FTVASTIASQIPHAVGAAYSMKM+KKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 325 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 384
           ICRNNGWAISTPISDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 385 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 444
           PILIEALTYR GHHTTSDDSTRYRPA+E+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRAGHHTTSDDSTRYRPAQEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAEL 420

Query: 445 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RS LRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480

[Close]

BLAST of MC01g0696 vs. NCBI nr
Match: XP_038883947.1 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 844 bits (2181), Expect = 1.67e-306
Identity = 417/482 (86.51%), Postives = 447/482 (92.74%), Query Frame = 0


Query: 25  MAFQARKAT-KIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 84
           M F  RKAT KIIPHF N M +FLGS+EMGF +SY +T L  T F+  KIG G +  EDM
Sbjct: 1   MVFHVRKATTKIIPHFRNGM-SFLGSLEMGFFNSY-STHLRNTSFAAYKIGTGALDSEDM 60

Query: 85  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 144
           PTHLSARRF+SIK  K+LDSVNDD DNQVLEFPGGKV+FT  +SFIP R++ERIPCFRVL
Sbjct: 61  PTHLSARRFESIKAGKQLDSVNDDCDNQVLEFPGGKVKFTSQLSFIPYREEERIPCFRVL 120

Query: 145 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 204
           DDNGQ S++SNFTQVSKEVA+KMY+DMVTLQTMDTI YEAQRQGRISFYLTS+GEEAINI
Sbjct: 121 DDNGQPSMYSNFTQVSKEVAMKMYSDMVTLQTMDTIFYEAQRQGRISFYLTSLGEEAINI 180

Query: 205 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 264
           ASAAALS DDLIVPQYREAGVLIWRGFTLKEFASQCF NK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSFDDLIVPQYREAGVLIWRGFTLKEFASQCFSNKYDYCKGRQMPVHYGSKRHNY 240

Query: 265 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 324
           FTVASTIASQIPHAVGAAYSMKM+K+DACAVTYFGDGGTSEGDFH ALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMDKRDACAVTYFGDGGTSEGDFHGALNFAAVLEAPVIF 300

Query: 325 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 384
           ICRNNGWAISTPISDQFRSDGV+VKGRAYGVRSIR+DGNDALAVYNAVRAAR+MAIQE +
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRIDGNDALAVYNAVRAARKMAIQEHK 360

Query: 385 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 444
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVAKDPVSRFRKWIESNGWW+NEAE+EL
Sbjct: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAKDPVSRFRKWIESNGWWNNEAEAEL 420

Query: 445 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RSSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSSLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480

[Close]

BLAST of MC01g0696 vs. ExPASy TrEMBL
Match: A0A6J1BTD3 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111005384 PE=4 SV=1)

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0


Query: 25  MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 84
           MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP
Sbjct: 1   MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 60

Query: 85  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 144
           THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD
Sbjct: 61  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 120

Query: 145 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 204
           DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA
Sbjct: 121 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 180

Query: 205 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 264
           SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 240

Query: 265 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 324
           TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 300

Query: 325 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 384
           CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 360

Query: 385 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 444
           ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 420

Query: 445 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 504
           SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP
Sbjct: 421 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 480

[Close]

BLAST of MC01g0696 vs. ExPASy TrEMBL
Match: A0A5A7SMQ8 (2-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00070 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 6.50e-311
Identity = 420/482 (87.14%), Postives = 451/482 (93.57%), Query Frame = 0


Query: 25  MAFQARKAT-KIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 84
           MAFQ RKAT KIIPHF N M  FLG +EMGF +SY +T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSY-STHLRNTSFSAHKIGTRILGSENM 60

Query: 85  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 144
           PTHL +RRF+SIKV K+LD VNDDRDNQVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120

Query: 145 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 204
           DDNGQ S++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180

Query: 205 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 264
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240

Query: 265 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 324
           FTVASTIASQIPHAVGAAYSMKM+KKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 325 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 384
           ICRNNGWAISTP+SDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 385 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 444
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETEL 420

Query: 445 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RSSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480

[Close]

BLAST of MC01g0696 vs. ExPASy TrEMBL
Match: A0A1S3B380 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103485287 PE=4 SV=1)

HSP 1 Score: 855 bits (2208), Expect = 6.50e-311
Identity = 420/482 (87.14%), Postives = 451/482 (93.57%), Query Frame = 0


Query: 25  MAFQARKAT-KIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 84
           MAFQ RKAT KIIPHF N M  FLG +EMGF +SY +T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQVRKATTKIIPHFRNGMAFFLGPLEMGFFNSY-STHLRNTSFSAHKIGTRILGSENM 60

Query: 85  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 144
           PTHL +RRF+SIKV K+LD VNDDRDNQVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLFSRRFESIKVGKQLDCVNDDRDNQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120

Query: 145 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 204
           DDNGQ S++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGQPSMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180

Query: 205 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 264
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240

Query: 265 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 324
           FTVASTIASQIPHAVGAAYSMKM+KKDACAV YFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVAYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 325 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 384
           ICRNNGWAISTP+SDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPVSDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 385 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 444
           PILIEALTYR GHHTTSDDSTRYRPAEE+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRAGHHTTSDDSTRYRPAEEMEWWRVAMDPVSRFRKWIESNGWWSNEAETEL 420

Query: 445 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RSSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSSLRKQLLEEIQLAERYEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480

[Close]

BLAST of MC01g0696 vs. ExPASy TrEMBL
Match: A0A0A0KF52 (E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483270 PE=4 SV=1)

HSP 1 Score: 846 bits (2186), Expect = 1.46e-307
Identity = 416/482 (86.31%), Postives = 450/482 (93.36%), Query Frame = 0


Query: 25  MAFQARKAT-KIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDM 84
           MAFQ RKAT KIIPHF N M  FLG +EMGF +SY +T L  T FS  KIG  ++G E+M
Sbjct: 1   MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSY-STHLRNTSFSAHKIGTRILGSENM 60

Query: 85  PTHLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVL 144
           PTHL +RRF+SIKV K+L+ VNDDRD+QVLEFPGGKV+FT  +SFIPDR++ERIPCFRVL
Sbjct: 61  PTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEERIPCFRVL 120

Query: 145 DDNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINI 204
           DDNG+  ++SNFTQVSKEVA+K+Y+DMVTLQTMDTI YEAQRQGRISFY+TS+GEEAIN+
Sbjct: 121 DDNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINV 180

Query: 205 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNY 264
           ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NY
Sbjct: 181 ASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNY 240

Query: 265 FTVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 324
           FTVASTIASQIPHAVGAAYSMKM+KKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF
Sbjct: 241 FTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIF 300

Query: 325 ICRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 384
           ICRNNGWAISTPISDQFRSDGV+VKG+AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR
Sbjct: 301 ICRNNGWAISTPISDQFRSDGVVVKGQAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQR 360

Query: 385 PILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESEL 444
           PILIEALTYR GHHTTSDDSTRYRPA+E+EWWRVA DPVSRFRKWIESNGWWSNEAE+EL
Sbjct: 361 PILIEALTYRAGHHTTSDDSTRYRPAQEMEWWRVAMDPVSRFRKWIESNGWWSNEAEAEL 420

Query: 445 RSSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RS LRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPSNF
Sbjct: 421 RSRLRKQLLEEIQLAERSEKPPVADAFTDVYDVPPSNLQEQEKWLRKTIKTHPQDYPSNF 480

[Close]

BLAST of MC01g0696 vs. ExPASy TrEMBL
Match: A0A6J1GE31 (2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111453112 PE=4 SV=1)

HSP 1 Score: 838 bits (2166), Expect = 9.92e-305
Identity = 411/481 (85.45%), Postives = 443/481 (92.10%), Query Frame = 0


Query: 25  MAFQARKATKIIPHFGNRMMNFLGSIEMGFASSYYATQLHKTPFSVQKIGNGVVGFEDMP 84
           MAF  RKATKIIPHF N M  FLGS             L +TPF+V KIGN V+GFE+M 
Sbjct: 1   MAFHVRKATKIIPHFRNGM-RFLGS-------------LRETPFAVHKIGNRVLGFENML 60

Query: 85  THLSARRFKSIKVEKRLDSVNDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLD 144
            HLS  RFKS KVEK++DSVNDDRDNQVL FPGGKV+FTP +SF+P+R++ER+PCFRVLD
Sbjct: 61  NHLSTHRFKSTKVEKQIDSVNDDRDNQVLNFPGGKVKFTPQLSFVPEREEERVPCFRVLD 120

Query: 145 DNGQASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIA 204
           DNGQ S++SNFTQVSKE+A+KMYNDMVTLQTMDTI YEAQRQGRISFY+TS+GEEAINIA
Sbjct: 121 DNGQPSMYSNFTQVSKEIAMKMYNDMVTLQTMDTIFYEAQRQGRISFYVTSLGEEAINIA 180

Query: 205 SAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYF 264
           SAAALSLDDLIVPQYREAGVL+WRGFTLKEFASQCFGNK D+CKGRQMPVHYGSKR+NYF
Sbjct: 181 SAAALSLDDLIVPQYREAGVLVWRGFTLKEFASQCFGNKYDYCKGRQMPVHYGSKRHNYF 240

Query: 265 TVASTIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFI 324
           TVASTIASQIPHAVGAAYSMKMEK+DACAVTYFGDGGTSEGDFHAALNFAAVLE PVIFI
Sbjct: 241 TVASTIASQIPHAVGAAYSMKMEKRDACAVTYFGDGGTSEGDFHAALNFAAVLEVPVIFI 300

Query: 325 CRNNGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRP 384
           CRNNGWAISTPISDQFRSDGV+VKGRAYGVRSIRVDGNDALAVYN VRAAR+MAIQE+RP
Sbjct: 301 CRNNGWAISTPISDQFRSDGVVVKGRAYGVRSIRVDGNDALAVYNTVRAARKMAIQERRP 360

Query: 385 ILIEALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELR 444
           ILIEALTYRVGHHTTSDDSTRYRP EE+EWWRVAKDPVSRFRKWIESNGWWS+EAE+ELR
Sbjct: 361 ILIEALTYRVGHHTTSDDSTRYRPVEEMEWWRVAKDPVSRFRKWIESNGWWSDEAEAELR 420

Query: 445 SSLRKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFP 504
           SSLRKQLLEEIQLAER EKPPVA+AFTDVYDVPPSNL+EQE WLRKTIKTHPQDYPS+F 
Sbjct: 421 SSLRKQLLEEIQLAERTEKPPVADAFTDVYDVPPSNLEEQERWLRKTIKTHPQDYPSDFQ 467

[Close]

BLAST of MC01g0696 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 634.4 bits (1635), Expect = 7.7e-182
Identity = 297/397 (74.81%), Postives = 353/397 (88.92%), Query Frame = 0


Query: 109 DNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNGQASIFSNFTQVSKEVAVKMYN 168
           ++QV++FPGGKV FTP + FI + DKER+PC+RVLDDNGQ    S F QVS+EVAVK+Y+
Sbjct: 76  NHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYS 135

Query: 169 DMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAAALSLDDLIVPQYREAGVLIWR 228
           DMVTLQ MD I YEAQRQGR+SFY T+IGEEAINIASAAAL+  D+I PQYRE GVL+WR
Sbjct: 136 DMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWR 195

Query: 229 GFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVASTIASQIPHAVGAAYSMKMEK 288
           GFTL+EFA+QCFGNK D+ KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KM+K
Sbjct: 196 GFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDK 255

Query: 289 KDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVIVK 348
           KDACAVTYFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGV+VK
Sbjct: 256 KDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVK 315

Query: 349 GRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRVGHHTTSDDSTRYRP 408
           GRAYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYRVGHH+TSDDSTRYR 
Sbjct: 316 GRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRS 375

Query: 409 AEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLRKQLLEEIQLAERREKPPVAE 468
           A EIEWW  A++P+SRFR WIESNGWWS++ ES+LRS ++K++LE +++AE+ EKP +  
Sbjct: 376 AGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQN 435

Query: 469 AFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFPL 506
            F+DVYDVPPSNL+EQE  +R+TI +HPQDYPS+ PL
Sbjct: 436 MFSDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 472

[Close]

BLAST of MC01g0696 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 633.6 bits (1633), Expect = 1.3e-181
Identity = 297/395 (75.19%), Postives = 351/395 (88.86%), Query Frame = 0


Query: 111 QVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNGQASIFSNFTQVSKEVAVKMYNDM 170
           QV++FPGGKV FTP + FI + DKER+PC+RVLDDNGQ    S F QVS+EVAVK+Y+DM
Sbjct: 7   QVMDFPGGKVAFTPEIQFISESDKERVPCYRVLDDNGQLITNSQFVQVSEEVAVKIYSDM 66

Query: 171 VTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAAALSLDDLIVPQYREAGVLIWRGF 230
           VTLQ MD I YEAQRQGR+SFY T+IGEEAINIASAAAL+  D+I PQYRE GVL+WRGF
Sbjct: 67  VTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIASAAALTPQDVIFPQYREPGVLLWRGF 126

Query: 231 TLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVASTIASQIPHAVGAAYSMKMEKKD 290
           TL+EFA+QCFGNK D+ KGRQMPVHYGS + NYFTV++TIA+Q+P+AVGAAYS+KM+KKD
Sbjct: 127 TLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPNAVGAAYSLKMDKKD 186

Query: 291 ACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPISDQFRSDGVIVKGR 350
           ACAVTYFGDGGTSEGDFHAALN AAV+EAPV+FICRNNGWAISTP SDQFRSDGV+VKGR
Sbjct: 187 ACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNGWAISTPTSDQFRSDGVVVKGR 246

Query: 351 AYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRVGHHTTSDDSTRYRPAE 410
           AYG+RSIRVDGNDALA+Y+AV  AREMAI+EQRPILIEALTYRVGHH+TSDDSTRYR A 
Sbjct: 247 AYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEALTYRVGHHSTSDDSTRYRSAG 306

Query: 411 EIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLRKQLLEEIQLAERREKPPVAEAF 470
           EIEWW  A++P+SRFR WIESNGWWS++ ES+LRS ++K++LE +++AE+ EKP +   F
Sbjct: 307 EIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKKEMLEALRVAEKTEKPNLQNMF 366

Query: 471 TDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNFPL 506
           +DVYDVPPSNL+EQE  +R+TI +HPQDYPS+ PL
Sbjct: 367 SDVYDVPPSNLREQELLVRQTINSHPQDYPSDVPL 401

[Close]

BLAST of MC01g0696 vs. TAIR 10
Match: AT1G21400.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 592.8 bits (1527), Expect = 2.6e-169
Identity = 279/416 (67.07%), Postives = 345/416 (82.93%), Query Frame = 0


Query: 91  RFKSIKVEKRLDSV---NDDRDNQVLEFPGGKVRFTPHMSFIPDRDKERIPCFRVLDDNG 150
           R +S  VE + D +    D+ D Q L+FPGGKV +T  M FIP+    RIPC+RVLD++G
Sbjct: 55  RHESTAVETQADHLVQQIDEVDAQELDFPGGKVGYTSEMKFIPESSSRRIPCYRVLDEDG 114

Query: 151 QASIFSNFTQVSKEVAVKMYNDMVTLQTMDTILYEAQRQGRISFYLTSIGEEAINIASAA 210
           +    S+F  VS+++AV+MY  M TLQ MD I YEAQRQGRISFYLTS+GEEAINIASAA
Sbjct: 115 RIIPDSDFIPVSEKLAVRMYEQMATLQVMDHIFYEAQRQGRISFYLTSVGEEAINIASAA 174

Query: 211 ALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVA 270
           ALS DD+++PQYRE GVL+WRGFTL+EFA+QCFGNK D+ KGRQMP+HYGS R NYFT++
Sbjct: 175 ALSPDDVVLPQYREPGVLLWRGFTLEEFANQCFGNKADYGKGRQMPIHYGSNRLNYFTIS 234

Query: 271 STIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRN 330
           S IA+Q+P A G  YS+KM+KK+AC VT+ GDGGTSEGDFHA LNFAAV+EAPV+FICRN
Sbjct: 235 SPIATQLPQAAGVGYSLKMDKKNACTVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRN 294

Query: 331 NGWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILI 390
           NGWAIST IS+QFRSDG++VKG+AYG+RSIRVDGNDALAVY+AVR+AREMA+ EQRP+LI
Sbjct: 295 NGWAISTHISEQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVRSAREMAVTEQRPVLI 354

Query: 391 EALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSL 450
           E +TYRVGHH+TSDDST+YR A+EI++W+++++PV+RFRKW+E NGWWS E ES+LRS+ 
Sbjct: 355 EMMTYRVGHHSTSDDSTKYRAADEIQYWKMSRNPVNRFRKWVEDNGWWSEEDESKLRSNA 414

Query: 451 RKQLLEEIQLAERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDYPSNF 504
           RKQLL+ IQ AE+ EK P+ E F DVYDV P NL+EQE  L++ +K  PQDYP  F
Sbjct: 415 RKQLLQAIQAAEKWEKQPLTELFNDVYDVKPKNLEEQELGLKELVKKQPQDYPPGF 470

[Close]

BLAST of MC01g0696 vs. TAIR 10
Match: AT5G34780.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.6e-86
Identity = 148/222 (66.67%), Postives = 188/222 (84.68%), Query Frame = 0


Query: 278 VGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNNGWAISTPIS 337
           +G + +    +K+ACAVT+ GDGGTSEGDFHA LNFAAV+EAPV+FICRNNGWAIST IS
Sbjct: 15  IGLSKAKDCWEKNACAVTFIGDGGTSEGDFHAGLNFAAVMEAPVVFICRNNGWAISTHIS 74

Query: 338 DQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIEALTYRVGHH 397
           +QFRSDG++VKG+AYG+RSIRVDGNDALAVY+AV +AREMA+ EQRP+LIE + YRVGHH
Sbjct: 75  EQFRSDGIVVKGQAYGIRSIRVDGNDALAVYSAVCSAREMAVTEQRPVLIEMMIYRVGHH 134

Query: 398 TTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLRKQLLEEIQL 457
           +TSDDST+YR A+EI++W+++++ V+RFRK +E NGWWS E ES+LRS+ RKQLL+ IQ 
Sbjct: 135 STSDDSTKYRAADEIQYWKMSRNSVNRFRKSVEDNGWWSEEDESKLRSNARKQLLQAIQA 194

Query: 458 AERREKPPVAEAFTDVYDVPPSNLKEQESWLRKTIKTHPQDY 500
           AE+ EK P+ E F DVYDV P NL+E+E  L++ I+  PQDY
Sbjct: 195 AEKWEKQPLTELFNDVYDVKPKNLEEEELGLKELIEKQPQDY 236

[Close]

BLAST of MC01g0696 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 9.8e-36
Identity = 94/326 (28.83%), Postives = 163/326 (50.00%), Query Frame = 0


Query: 153 SNFTQVSKEVAVKMYNDMVTLQTMDTI---LYEAQR-QGRISFYLTSIGEEAINIASAAA 212
           S   + S E  +  + DM  ++ M+     LY+A+  +G    Y    G+EA+ +   AA
Sbjct: 53  SRSVETSSEEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLY---DGQEALAVGMEAA 112

Query: 213 LSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKDDHCKGRQMPVHYGSKRYNYFTVAS 272
           ++  D I+  YR+    I RG  L +  S+  G K     G+   +H+  K  +++    
Sbjct: 113 ITKKDAIITSYRDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHG 172

Query: 273 TIASQIPHAVGAAYSMKMEKKDACAVTYFGDGGTSEGDFHAALNFAAVLEAPVIFICRNN 332
            + +QIP   G A++ K  K +A     +GDG  ++G    ALN +A+ + P I +C NN
Sbjct: 173 IVGAQIPLGCGLAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENN 232

Query: 333 GWAISTPISDQFRSDGVIVKGRAYGVRSIRVDGNDALAVYNAVRAAREMAIQEQRPILIE 392
            + + T      +S     +G    V  ++VDG DALAV  A + A+E A+ +  PI++E
Sbjct: 233 HYGMGTATWRSAKSPAYFKRGDY--VPGLKVDGMDALAVKQACKFAKEHAL-KNGPIILE 292

Query: 393 ALTYRVGHHTTSDDSTRYRPAEEIEWWRVAKDPVSRFRKWIESNGWWSNEAESELRSSLR 452
             TYR   H+ SD  + YR  +EI   R  +DP+ R RK + ++   + +   ++   +R
Sbjct: 293 MDTYRYHGHSMSDPGSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIR 352

Query: 453 KQLLEEIQLAERREKPPVAEAFTDVY 475
           K++ + +  A+    P  +E FT++Y
Sbjct: 353 KEVDDAVAQAKESPIPDASELFTNMY 372

The following BLAST results are available for this feature:

BLAST of MC01g0696 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84JL2	1.1e-180	74.81	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Arabidopsis tha...	[more]
Q9LPL5	3.6e-168	67.07	2-oxoisovalerate dehydrogenase subunit alpha 1, mitochondrial OS=Arabidopsis tha...	[more]
Q54M22	5.7e-113	51.62	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Dictyostelium dis...	[more]
P50136	9.1e-111	47.09	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Mus musculus OX=1...	[more]
A5A6H9	1.5e-110	49.61	2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial OS=Pan troglodytes O...	[more]

BLAST of MC01g0696 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132554.1	0.0	100.00	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Momordica charant...	[more]
XP_008441060.1	1.34e-310	87.14	PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucumi...	[more]
XP_023543630.1	2.62e-307	86.28	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Cucurbita pepo su...	[more]
XP_004148662.1	3.01e-307	86.31	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial isoform X1 [Cucumi...	[more]
XP_038883947.1	1.67e-306	86.51	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial [Benincasa hispida...	[more]

BLAST of MC01g0696 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTD3	0.0	100.00	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Momordica chara...	[more]
A0A5A7SMQ8	6.50e-311	87.14	2-oxoisovalerate dehydrogenase subunit alpha 2 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3B380	6.50e-311	87.14	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucumis melo OX...	[more]
A0A0A0KF52	1.46e-307	86.31	E1_dh domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G483270 PE=4 ...	[more]
A0A6J1GE31	9.92e-305	85.45	2-oxoisovalerate dehydrogenase subunit alpha 2, mitochondrial OS=Cucurbita mosch...	[more]

BLAST of MC01g0696 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09300.1	7.7e-182	74.81	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	1.3e-181	75.19	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT1G21400.1	2.6e-169	67.07	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G34780.1	1.6e-86	66.67	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT1G24180.1	9.8e-36	28.83	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 170..465 e-value: 5.5E-96 score: 321.0
None	No IPR available	GENE3D	3.40.50.970		coord: 108..503 e-value: 1.0E-144 score: 484.3
None	No IPR available	PANTHER	PTHR43380	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA, MITOCHONDRIAL	coord: 87..505
None	No IPR available	PANTHER	PTHR43380:SF11	2-OXOISOVALERATE DEHYDROGENASE SUBUNIT ALPHA 2, MITOCHONDRIAL	coord: 87..505
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 166..458 e-value: 4.69916E-148 score: 423.445
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 153..501

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0696.1	MC01g0696.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009083	branched-chain amino acid catabolic process
cellular_component	GO:0005947	mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor