MC01g0691 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0691
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	cysteine proteinase inhibitor 5-like
Location	MC01: 13083167 .. 13083523 (-)
RNA-Seq Expression	MC01g0691
Synteny	MC01g0691

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCTGTGGCAGTCGCCCCCGCACAAAACGATGCATTGCAACATGTCAAAAAGCCTGGTGGTTGGGAGCCGATTAAGAACATCAAGGACCCATATGTGCAAGAGATGGGAAGGTTTGCAGTGATGGAACAGCCTAAGCCAGTAGGGCTCGTGGCATCGATGGAGTTTGTCGGTGTGGTGAGCGGCGAGATTCAGCTGGTGGCGGCTGGAAAAGAATACCGGCTTGTGGTGGAGGTGAAGGTGACAGAAAAGATAATAGTTCATGGTTGCCCTGTAAGTTCCATTAGACTTTACGTTGCTGTTGTGCTGGACAAGCCAAGTGACAAGTGCACCACTCGAGCTGCCTGTTGGCCT

mRNA sequence

ATGGATTCTGTGGCAGTCGCCCCCGCACAAAACGATGCATTGCAACATGTCAAAAAGCCTGGTGGTTGGGAGCCGATTAAGAACATCAAGGACCCATATGTGCAAGAGATGGGAAGGTTTGCAGTGATGGAACAGCCTAAGCCAGTAGGGCTCGTGGCATCGATGGAGTTTGTCGGTGTGGTGAGCGGCGAGATTCAGCTGGTGGCGGCTGGAAAAGAATACCGGCTTGTGGTGGAGGTGAAGGTGACAGAAAAGATAATAGTTCATGGTTGCCCTGTAAGTTCCATTAGACTTTACGTTGCTGTTGTGCTGGACAAGCCAAGTGACAAGTGCACCACTCGAGCTGCCTGTTGGCCT

Coding sequence (CDS)

ATGGATTCTGTGGCAGTCGCCCCCGCACAAAACGATGCATTGCAACATGTCAAAAAGCCTGGTGGTTGGGAGCCGATTAAGAACATCAAGGACCCATATGTGCAAGAGATGGGAAGGTTTGCAGTGATGGAACAGCCTAAGCCAGTAGGGCTCGTGGCATCGATGGAGTTTGTCGGTGTGGTGAGCGGCGAGATTCAGCTGGTGGCGGCTGGAAAAGAATACCGGCTTGTGGTGGAGGTGAAGGTGACAGAAAAGATAATAGTTCATGGTTGCCCTGTAAGTTCCATTAGACTTTACGTTGCTGTTGTGCTGGACAAGCCAAGTGACAAGTGCACCACTCGAGCTGCCTGTTGGCCT

Protein sequence

MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDKCTTRAACWP

Homology

[Close]

BLAST of MC01g0691 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 1.9e-06
Identity = 38/87 (43.68%), Postives = 48/87 (55.17%), Query Frame = 0


Query: 21  GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVEV 80
           GGW PI N+ DP V E+G FAV E  K     + ++F  VVSGE Q+V +G  YRL V  
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNK--RSESGLKFETVVSGETQVV-SGTNYRLKVAA 88

Query: 81  KVTEKIIVHGCPVSSIRLYVAVVLDKP 108
                    G  VS  + Y+A+V DKP
Sbjct: 89  N-------DGDGVS--KNYLAIVWDKP 103

[Close]

BLAST of MC01g0691 vs. ExPASy Swiss-Prot
Match: Q10Q46 (Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210200 PE=3 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 7.1e-06
Identity = 28/60 (46.67%), Postives = 37/60 (61.67%), Query Frame = 0


Query: 20 PGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVE 79
          P GW PIKNI DP++QE+GR+A+ E  + V     + F  V  GE Q V +G  YRL +E
Sbjct: 20 PRGWSPIKNIDDPHIQELGRWAITENNR-VSPSDELTFHRVTGGE-QQVVSGMNYRLEIE 77

[Close]

BLAST of MC01g0691 vs. ExPASy Swiss-Prot
Match: Q10Q47 (Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0210100 PE=3 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 7.8e-05
Identity = 28/59 (47.46%), Postives = 38/59 (64.41%), Query Frame = 0


Query: 21 GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVE 80
          G WEP+ +I DP+VQE+GR+AV E+ + V     + F  V  GE Q+V AG  YRL +E
Sbjct: 28 GSWEPV-DINDPHVQELGRWAVAEEDRGVA-AGGLTFERVTDGEKQVV-AGVNYRLTLE 83

[Close]

BLAST of MC01g0691 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 5.1e-04
Identity = 35/104 (33.65%), Postives = 46/104 (44.23%), Query Frame = 0


Query: 3   SVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVS 62
           S A A        H    GGW PI ++ DP++QE+G +AV            + F  V S
Sbjct: 15  SAAAAAQVGGNRGHGPLVGGWSPITDVGDPHIQELGGWAVERHASLSS--DGLRFRRVTS 74

Query: 63  GEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDK 107
           GE Q V +G  YRLVV             P  +   YVAVV ++
Sbjct: 75  GE-QQVVSGMNYRLVVSA---------SDPAGATASYVAVVYEQ 106

[Close]

BLAST of MC01g0691 vs. NCBI nr
Match: XP_022132703.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 216 bits (551), Expect = 6.58e-70
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0


Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 60
           MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV
Sbjct: 32  MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 91

Query: 61  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
           VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK
Sbjct: 92  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 141

[Close]

BLAST of MC01g0691 vs. NCBI nr
Match: XP_022132702.1 (cysteine proteinase inhibitor 5-like [Momordica charantia])

HSP 1 Score: 196 bits (498), Expect = 3.04e-62
Identity = 101/111 (90.99%), Postives = 105/111 (94.59%), Query Frame = 0


Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQ-PKPVGLVASMEFVG 60
           MDSVAVAPAQNDAL H+K PGGWEPIKNIKDPYVQEMGRFAVMEQ PKP  L+ASMEF+G
Sbjct: 1   MDSVAVAPAQNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELIASMEFIG 60

Query: 61  VVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
           VVSGEIQLVA GKEYRLVVEV+VTEKIIVHGCPVSSIRLYVAVVLDKPSDK
Sbjct: 61  VVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 111

[Close]

BLAST of MC01g0691 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 112 bits (280), Expect = 3.14e-29
Identity = 69/112 (61.61%), Postives = 83/112 (74.11%), Query Frame = 0


Query: 1   MDSVAVAPA--QNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFV 60
           MD VAVAPA  QNDAL +V+KPGGWEPIKNI DPYVQE+GRFAVME    +   AS EF+
Sbjct: 1   MDPVAVAPAVQQNDALLNVQKPGGWEPIKNINDPYVQELGRFAVMEHTHNL-CGASYEFI 60

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
            VVSGE Q+V  GK+Y LV+EVK  E II+   P SSI+ + A++LDK  +K
Sbjct: 61  RVVSGESQVVG-GKKYCLVIEVK--ETIIIPSMP-SSIKFFKAIMLDKAWEK 107

[Close]

BLAST of MC01g0691 vs. NCBI nr
Match: WP_198843152.1 (hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical protein [Aureibaculum sp. A20])

HSP 1 Score: 110 bits (274), Expect = 2.79e-28
Identity = 69/115 (60.00%), Postives = 82/115 (71.30%), Query Frame = 0


Query: 1   MDSVAVAPAQNDAL-QHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLVASMEFV 60
           MD   +AP QNDAL Q+  KPGGWEPIKNI DPYVQEMGRF+VME   K VG  ASM+F+
Sbjct: 5   MDFEVIAPGQNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTHKLVG--ASMKFI 64

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVS---SIRLYVAVVLDKPSDK 110
            VVSGE + ++ GKEYRLVVEV   E II   CP     S++ Y+A VL+KP D+
Sbjct: 65  RVVSGETRPMSEGKEYRLVVEV--AEHIIT--CPPMLSVSVKFYLATVLEKPLDR 113

[Close]

BLAST of MC01g0691 vs. NCBI nr
Match: WP_156424223.1 (hypothetical protein, partial [Myroides odoratus])

HSP 1 Score: 107 bits (267), Expect = 2.09e-27
Identity = 67/110 (60.91%), Postives = 80/110 (72.73%), Query Frame = 0


Query: 6   VAPAQNDAL-QHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQP-KPVGLVASMEFVGVVSG 65
           +AP QNDAL Q+  KPGGWEPIKNI DPYVQEMGRF+VME   K VG  ASM+F+ VVSG
Sbjct: 3   IAPGQNDALHQNRCKPGGWEPIKNIHDPYVQEMGRFSVMEHTHKLVG--ASMKFIRVVSG 62

Query: 66  EIQLVAAGKEYRLVVEVKVTEKIIVHGCPVS---SIRLYVAVVLDKPSDK 110
           E + ++ GKEYRLVVEV   E II   CP     S++ Y+A VL+KP D+
Sbjct: 63  ETRPMSEGKEYRLVVEV--AEHIIT--CPPMLSVSVKFYLATVLEKPLDR 106

[Close]

BLAST of MC01g0691 vs. ExPASy TrEMBL
Match: A0A6J1BX23 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005501 PE=4 SV=1)

HSP 1 Score: 216 bits (551), Expect = 3.19e-70
Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 0


Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 60
           MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV
Sbjct: 32  MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 91

Query: 61  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
           VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK
Sbjct: 92  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 141

[Close]

BLAST of MC01g0691 vs. ExPASy TrEMBL
Match: A0A6J1BUK6 (cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005500 PE=4 SV=1)

HSP 1 Score: 196 bits (498), Expect = 1.47e-62
Identity = 101/111 (90.99%), Postives = 105/111 (94.59%), Query Frame = 0


Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQ-PKPVGLVASMEFVG 60
           MDSVAVAPAQNDAL H+K PGGWEPIKNIKDPYVQEMGRFAVMEQ PKP  L+ASMEF+G
Sbjct: 1   MDSVAVAPAQNDALLHLKMPGGWEPIKNIKDPYVQEMGRFAVMEQHPKPSELIASMEFIG 60

Query: 61  VVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
           VVSGEIQLVA GKEYRLVVEV+VTEKIIVHGCPVSSIRLYVAVVLDKPSDK
Sbjct: 61  VVSGEIQLVAHGKEYRLVVEVRVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 111

[Close]

BLAST of MC01g0691 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 112 bits (280), Expect = 1.52e-29
Identity = 69/112 (61.61%), Postives = 83/112 (74.11%), Query Frame = 0


Query: 1   MDSVAVAPA--QNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFV 60
           MD VAVAPA  QNDAL +V+KPGGWEPIKNI DPYVQE+GRFAVME    +   AS EF+
Sbjct: 1   MDPVAVAPAVQQNDALLNVQKPGGWEPIKNINDPYVQELGRFAVMEHTHNL-CGASYEFI 60

Query: 61  GVVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
            VVSGE Q+V  GK+Y LV+EVK  E II+   P SSI+ + A++LDK  +K
Sbjct: 61  RVVSGESQVVG-GKKYCLVIEVK--ETIIIPSMP-SSIKFFKAIMLDKAWEK 107

[Close]

BLAST of MC01g0691 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 4.08e-21
Identity = 59/111 (53.15%), Postives = 70/111 (63.06%), Query Frame = 0


Query: 1   MDSVAVAPAQNDALQHVKKPGGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVA-SMEFVG 60
           MD   VA  QNDAL H  K   WEP+ N+ D YVQE+GRFAVME     GL    M F+ 
Sbjct: 1   MDLKVVAHGQNDALLHKPKSCDWEPV-NVHDQYVQELGRFAVMEHVH--GLCGVGMTFIC 60

Query: 61  VVSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
           VVSGE ++V  GK+Y LVVEVK    ++ H CP  +I+LYVA VLDK   K
Sbjct: 61  VVSGESKMVCEGKKYSLVVEVKEVP-VLPHACP--AIKLYVADVLDKQCAK 105

[Close]

BLAST of MC01g0691 vs. ExPASy TrEMBL
Match: A0A6J1FCN5 (cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC111442890 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 8.90e-15
Identity = 49/110 (44.55%), Postives = 69/110 (62.73%), Query Frame = 0


Query: 4   VAVAPAQNDALQHVKKP---GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGV 63
           V  AP QN+A+   KK    G W+PI++IKDPYVQEMGRFAVME    + +  +++F+ V
Sbjct: 5   VEAAPGQNEAMFQDKKGIEVGQWKPIEDIKDPYVQEMGRFAVMEHDN-MRIGENLKFICV 64

Query: 64  VSGEIQLVAAGKEYRLVVEVKVTEKIIVHGCPVSSIRLYVAVVLDKPSDK 110
            SGE Q+V  G  YRL+++         +   +SS  LY+A+V DKP +K
Sbjct: 65  QSGEKQVVE-GMNYRLLIKAS-------NHLGISS--LYMAIVYDKPWEK 103

[Close]

BLAST of MC01g0691 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.3e-07
Identity = 38/87 (43.68%), Postives = 48/87 (55.17%), Query Frame = 0


Query: 21  GGWEPIKNIKDPYVQEMGRFAVMEQPKPVGLVASMEFVGVVSGEIQLVAAGKEYRLVVEV 80
           GGW PI N+ DP V E+G FAV E  K     + ++F  VVSGE Q+V +G  YRL V  
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNK--RSESGLKFETVVSGETQVV-SGTNYRLKVAA 88

Query: 81  KVTEKIIVHGCPVSSIRLYVAVVLDKP 108
                    G  VS  + Y+A+V DKP
Sbjct: 89  N-------DGDGVS--KNYLAIVWDKP 103

The following BLAST results are available for this feature:

BLAST of MC01g0691 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41916	1.9e-06	43.68	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]
Q10Q46	7.1e-06	46.67	Cysteine proteinase inhibitor 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
Q10Q47	7.8e-05	47.46	Putative cysteine proteinase inhibitor 7 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q10J94	5.1e-04	33.65	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]

BLAST of MC01g0691 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132703.1	6.58e-70	100.00	cysteine proteinase inhibitor 5-like [Momordica charantia]	[more]
XP_022132702.1	3.04e-62	90.99	cysteine proteinase inhibitor 5-like [Momordica charantia]	[more]
XP_022132557.1	3.14e-29	61.61	cysteine proteinase inhibitor 6-like [Momordica charantia]	[more]
WP_198843152.1	2.79e-28	60.00	hypothetical protein, partial [Aureibaculum sp. A20] >MBJ2176574.1 hypothetical ...	[more]
WP_156424223.1	2.09e-27	60.91	hypothetical protein, partial [Myroides odoratus]	[more]

BLAST of MC01g0691 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BX23	3.19e-70	100.00	cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1BUK6	1.47e-62	90.99	cysteine proteinase inhibitor 5-like OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1BU61	1.52e-29	61.61	cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1GCT2	4.08e-21	53.15	uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC1114530...	[more]
A0A6J1FCN5	8.90e-15	44.55	cysteine proteinase inhibitor 8-like OS=Cucurbita moschata OX=3662 GN=LOC1114428...	[more]

BLAST of MC01g0691 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	1.3e-07	43.68	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 26..109 e-value: 2.2E-10 score: 40.8
None	No IPR available	GENE3D	3.10.450.10		coord: 17..116 e-value: 1.6E-16 score: 62.2
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 21..109
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 13..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0691.1	MC01g0691.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity