MC01g0588 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g0588 vs. ExPASy Swiss-Prot
Match: Q9FFN0 (GDSL esterase/lipase At5g03810 OS=Arabidopsis thaliana OX=3702 GN=At5g03810 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 2.9e-136
Identity = 237/332 (71.39%), Postives = 277/332 (83.43%), Query Frame = 0

Query: 68  VATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDF 127
           V TG+ LVPAL I GDSVVD GNNN+ +T+VKANFPPYGRDFV H+ TGRF NGKLATDF
Sbjct: 21  VGTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHSATGRFSNGKLATDF 80

Query: 128 TAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEY 187
           TAE+LGF+SYP AYLSQ+A    LLTGANFAS ASGF D TA  Y+AI+L+QQL  YKEY
Sbjct: 81  TAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYNAITLSQQLKNYKEY 140

Query: 188 QNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITSYST 247
           QNKV N+VG+ + N IFSGAIHLLS GSSDF+Q+YY+NP+L R ++P Q+SD L+ SYST
Sbjct: 141 QNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFTPDQYSDHLLRSYST 200

Query: 248 FIQNLYGLGARRIGVTGLPPLGCLPAAITLFCS-GSNQCIQRLNQDAIAFNTKLKSTSQS 307
           F+QNLYGLGARRIGVT LPPLGCLPAAITLF   G+N C++RLNQDA++FNTKL +TS +
Sbjct: 201 FVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQDAVSFNTKLNNTSIN 260

Query: 308 LQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGSIGTCSN 367
           L N    LKLVVFDIY PLLNM+  P E GFFESRRACCGTGT+ETS LCN+ S+GTCSN
Sbjct: 261 LTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMETSFLCNALSVGTCSN 320

Query: 368 ATGYVFWDGFHPTESANQVLAGDLISQGFALI 399
           AT YVFWDGFHP+E+AN+V+A +L+ QG  LI
Sbjct: 321 ATNYVFWDGFHPSEAANRVIANNLLVQGIPLI 352

BLAST of MC01g0588 vs. ExPASy Swiss-Prot
Match: Q9LZC5 (GDSL esterase/lipase At5g03820 OS=Arabidopsis thaliana OX=3702 GN=At5g03820 PE=3 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 6.4e-136
Identity = 239/343 (69.68%), Postives = 277/343 (80.76%), Query Frame = 0

Query: 59  FSLLA-LVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGR 118
           FS++A     V TG+PLVPAL I GDSVVD GNNN L T++KANFPPYGRDF+ H  TGR
Sbjct: 11  FSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNATGR 70

Query: 119 FCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISL 178
           F NGKLATDFTAESLGF+SYP  YLSQ+A G  LLTGANFAS ASG+ DGTA  Y+AI+L
Sbjct: 71  FSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNAITL 130

Query: 179 TQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQF 238
            QQL  YKEYQNKV N+VG  + N IFSGAIHLLS GSSDF+Q+YY+NP+L R ++P Q+
Sbjct: 131 NQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTPDQY 190

Query: 239 SDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLF--CSGSNQCIQRLNQDAIA 298
           SD L+  YSTF+QNLY LGAR+IGVT LPPLGCLPAAITLF     +N C++RLNQDA++
Sbjct: 191 SDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTCVERLNQDAVS 250

Query: 299 FNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLL 358
           FNTKL +TS +L N    LKLVVFDIY PLLNM   P ENGFFESRRACCGTGTVETS L
Sbjct: 251 FNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACCGTGTVETSFL 310

Query: 359 CNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 399
           CN+ S+GTCSNAT YVFWDGFHP+E+AN+V+A +L+ QG  LI
Sbjct: 311 CNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLI 353

BLAST of MC01g0588 vs. ExPASy Swiss-Prot
Match: Q0WPI9 (GDSL esterase/lipase At3g53100 OS=Arabidopsis thaliana OX=3702 GN=At3g53100 PE=2 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 7.6e-129
Identity = 214/325 (65.85%), Postives = 270/325 (83.08%), Query Frame = 0

Query: 74  LVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDFTAESLG 133
           +VPAL +FGDS+VDVGNNNNLL++VK+NF PYGRDF+   PTGRFCNGKLA DF+AE LG
Sbjct: 26  VVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRDFIDQRPTGRFCNGKLAVDFSAEYLG 85

Query: 134 FSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEYQNKVVN 193
           FSSYPPA+LS++A+   +L GANFASA+SG+YD T+  + +ISLT+QL+YY+ YQN+V  
Sbjct: 86  FSSYPPAFLSREASNENILIGANFASASSGYYDATSVPFGSISLTRQLSYYRAYQNRVTR 145

Query: 194 MVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITSYSTFIQNLY 253
           M+GRG    +FS  IH+LSAGSSDF+QNYY+NPLL    +P QF+DIL+ S+S FIQNLY
Sbjct: 146 MIGRGNARILFSRGIHILSAGSSDFLQNYYINPLLNILNTPDQFADILLRSFSEFIQNLY 205

Query: 254 GLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKLKSTSQSLQNKFSD 313
            LGARRIGV  LPP+GCLPAAITLF +G+  C++RLN DAI FNTKL++T++ L N+ S 
Sbjct: 206 ELGARRIGVISLPPMGCLPAAITLFGAGNKSCVERLNNDAIMFNTKLENTTRLLMNRHSG 265

Query: 314 LKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGSIGTCSNATGYVFW 373
           L+LV F++YQP L++I+ P +NGFFE++RACCGTGT+ETS LCNS S GTC NATGYVFW
Sbjct: 266 LRLVAFNVYQPFLDIITNPTDNGFFETKRACCGTGTIETSFLCNSLSFGTCVNATGYVFW 325

Query: 374 DGFHPTESANQVLAGDLISQGFALI 399
           DGFHPTE+ N++LAG L+ QG +LI
Sbjct: 326 DGFHPTEAVNELLAGQLLGQGISLI 350

BLAST of MC01g0588 vs. ExPASy Swiss-Prot
Match: Q9FFC6 (GDSL esterase/lipase At5g22810 OS=Arabidopsis thaliana OX=3702 GN=At5g22810 PE=3 SV=3)

HSP 1 Score: 456.4 bits (1173), Expect = 3.2e-127
Identity = 222/338 (65.68%), Postives = 269/338 (79.59%), Query Frame = 0

Query: 64  LVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKL 123
           +V+ V    PLVPA+ IFGDSVVDVGNNN++ T+VKANFPPYGRDF THTPTGRFCNGKL
Sbjct: 24  VVMVVMKAQPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKL 83

Query: 124 ATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNY 183
           ATDFTAE+LGF SYP AYLS+ A G  LL GANFASAASG+YDGTA+LY AISL QQL +
Sbjct: 84  ATDFTAENLGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEH 143

Query: 184 YKEYQNKVVNMV---GRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI 243
           YK+Y +++  +         ++I S  I+++SAGSSDFIQNYY+NPLLYR  SP +FSD+
Sbjct: 144 YKDYISRIQEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDL 203

Query: 244 LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL 303
           LI SYS+FIQNLY LGARRIGVT LPPLGCLPAAIT+       C ++LN DAI+FN KL
Sbjct: 204 LILSYSSFIQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKL 263

Query: 304 KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS 363
            +TSQ L+     L LVVFDIYQPL ++ ++P+E GF E+RRACCGTG +ETS+LCN  S
Sbjct: 264 NTTSQDLKRNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKS 323

Query: 364 IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 399
           +GTC+NAT YVFWDGFHPTE+AN++LA +L+  G +LI
Sbjct: 324 VGTCNNATEYVFWDGFHPTEAANKILADNLLVSGISLI 361

BLAST of MC01g0588 vs. ExPASy Swiss-Prot
Match: Q9LU14 (GDSL esterase/lipase APG OS=Arabidopsis thaliana OX=3702 GN=APG PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-114
Identity = 201/326 (61.66%), Postives = 249/326 (76.38%), Query Frame = 0

Query: 74  LVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDFTAESLG 133
           LVPA+  FGDSVVDVGNNN L T+ +A++PPYGRDF  H  TGRFCNGKLATD TAE+LG
Sbjct: 27  LVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFANHKATGRFCNGKLATDITAETLG 86

Query: 134 FSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEYQNKVVN 193
           F+ YPPAYLS +A+G  LL GANFASAASG+ D  A L HAI L QQ+ Y+KEY++K++ 
Sbjct: 87  FTKYPPAYLSPEASGKNLLIGANFASAASGYDDKAALLNHAIPLYQQVEYFKEYKSKLIK 146

Query: 194 MVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITSYSTFIQNLY 253
           + G  K +SI  GAI LLSAGSSDF+QNYYVNPLLY+ Y+   +   LI ++STFI+ +Y
Sbjct: 147 IAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPLLYKVYTVDAYGSFLIDNFSTFIKQVY 206

Query: 254 GLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKLKSTSQSLQNKFSD 313
            +GAR+IGVT LPP GCLPAA TLF      C+ RLN DA  FN KL + +  LQ ++SD
Sbjct: 207 AVGARKIGVTSLPPTGCLPAARTLFGFHEKGCVSRLNTDAQNFNKKLNAAASKLQKQYSD 266

Query: 314 LKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVE-TSLLCNSGSIGTCSNATGYVF 373
           LK+VVFDIY PL +++  P+++GF E+ + CCGTGTVE TSLLCN  S GTCSNAT YVF
Sbjct: 267 LKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGTGTVETTSLLCNPKSFGTCSNATQYVF 326

Query: 374 WDGFHPTESANQVLAGDLISQGFALI 399
           WD  HP+E+AN++LA  LI QGF+L+
Sbjct: 327 WDSVHPSEAANEILATALIGQGFSLL 352

BLAST of MC01g0588 vs. NCBI nr
Match: XP_022143974.1 (GDSL esterase/lipase At5g03810-like [Momordica charantia])

HSP 1 Score: 800 bits (2067), Expect = 2.44e-292
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MLPEAKFVHKFSPTCPLHLHSLSLLLPLLPKPKGFTILALERKMQLPPDAGAAAFWIPFS 60
           MLPEAKFVHKFSPTCPLHLHSLSLLLPLLPKPKGFTILALERKMQLPPDAGAAAFWIPFS
Sbjct: 1   MLPEAKFVHKFSPTCPLHLHSLSLLLPLLPKPKGFTILALERKMQLPPDAGAAAFWIPFS 60

Query: 61  LLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCN 120
           LLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCN
Sbjct: 61  LLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCN 120

Query: 121 GKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQ 180
           GKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQ
Sbjct: 121 GKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQ 180

Query: 181 LNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI 240
           LNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI
Sbjct: 181 LNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI 240

Query: 241 LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL 300
           LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL
Sbjct: 241 LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL 300

Query: 301 KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS 360
           KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS
Sbjct: 301 KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS 360

Query: 361 IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI
Sbjct: 361 IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398

BLAST of MC01g0588 vs. NCBI nr
Match: XP_022972797.1 (GDSL esterase/lipase At5g03820-like [Cucurbita maxima])

HSP 1 Score: 646 bits (1666), Expect = 5.36e-232
Identity = 319/355 (89.86%), Postives = 335/355 (94.37%), Query Frame = 0

Query: 44  MQLPPDAGAAAFWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 103
           MQLP    AA F + FSLLALV PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFP
Sbjct: 1   MQLP----AAGFLVTFSLLALVFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 60

Query: 104 PYGRDFVTHTPTGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASG 163
           PYGRDFVTH PTGRFCNGKLATDFTAE LGFSSYPPAYL QDATGN+LLTGANFASAASG
Sbjct: 61  PYGRDFVTHAPTGRFCNGKLATDFTAELLGFSSYPPAYLGQDATGNKLLTGANFASAASG 120

Query: 164 FYDGTAQLYHAISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYY 223
           FYDGTAQLYH+ISLTQQLNYYKEYQ+KVVNMVG GKGN+IFSG IHLLSAGSSDFIQNYY
Sbjct: 121 FYDGTAQLYHSISLTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDFIQNYY 180

Query: 224 VNPLLYRTYSPAQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSN 283
           VNPLLYRTYSP QFSDILITSYS FIQNLYGLGARRIGVTGLPP+GCLPAAITLF SGSN
Sbjct: 181 VNPLLYRTYSPQQFSDILITSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGSN 240

Query: 284 QCIQRLNQDAIAFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRA 343
           QCIQRLNQDA+AFNTKL+STSQSLQN+FSDLKLVVFDIYQPLLNM+SKPA+NGFFE+RRA
Sbjct: 241 QCIQRLNQDAMAFNTKLQSTSQSLQNRFSDLKLVVFDIYQPLLNMVSKPADNGFFEARRA 300

Query: 344 CCGTGTVETSLLCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           CCGTGTVETSLLCN+GSIGTCSN TGYVFWDGFHPTE+AN+VLA DLISQGF+LI
Sbjct: 301 CCGTGTVETSLLCNNGSIGTCSNVTGYVFWDGFHPTEAANKVLAADLISQGFSLI 351

BLAST of MC01g0588 vs. NCBI nr
Match: XP_022962729.1 (GDSL esterase/lipase At5g03820-like [Cucurbita moschata])

HSP 1 Score: 645 bits (1665), Expect = 7.61e-232
Identity = 318/355 (89.58%), Postives = 336/355 (94.65%), Query Frame = 0

Query: 44  MQLPPDAGAAAFWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 103
           MQLP    A  F + FSLLALV PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFP
Sbjct: 1   MQLP----AGGFLVTFSLLALVFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 60

Query: 104 PYGRDFVTHTPTGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASG 163
           PYGRDFVTH PTGRFCNGKLATDFTAE LGFSSYPPAYLSQDATGN+LLTGANFASAASG
Sbjct: 61  PYGRDFVTHAPTGRFCNGKLATDFTAEFLGFSSYPPAYLSQDATGNKLLTGANFASAASG 120

Query: 164 FYDGTAQLYHAISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYY 223
           FYDGTAQLYH+I+LTQQLNYYKEYQ+KVVNMVG GKGN+IFSG IHLLSAGSSD+IQNYY
Sbjct: 121 FYDGTAQLYHSIALTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDYIQNYY 180

Query: 224 VNPLLYRTYSPAQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSN 283
           VNPLLYRTYSP QFSDILITSYS FIQNLYGLGARRIGVTGLPP+GCLPAAITLF SGSN
Sbjct: 181 VNPLLYRTYSPQQFSDILITSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGSN 240

Query: 284 QCIQRLNQDAIAFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRA 343
           QCIQRLNQDA+AFNTKL+STSQSLQN+FSDLKLVVFDIYQPLLNM+SKPA+NGFFE+RRA
Sbjct: 241 QCIQRLNQDALAFNTKLQSTSQSLQNRFSDLKLVVFDIYQPLLNMVSKPADNGFFEARRA 300

Query: 344 CCGTGTVETSLLCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           CCGTGTVETSLLCN+GSIGTCSNATGYVFWDGFHPTE+AN+VLA DLISQGF+LI
Sbjct: 301 CCGTGTVETSLLCNNGSIGTCSNATGYVFWDGFHPTEAANKVLASDLISQGFSLI 351

BLAST of MC01g0588 vs. NCBI nr
Match: XP_038881618.1 (GDSL esterase/lipase At5g03820 [Benincasa hispida])

HSP 1 Score: 645 bits (1664), Expect = 1.08e-231
Identity = 319/355 (89.86%), Postives = 336/355 (94.65%), Query Frame = 0

Query: 44  MQLPPDAGAAAFWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 103
           MQLP     + F +PFSLLAL+ PVA   PLVPALCIFGDSVVDVGNNNNLLTVVKANFP
Sbjct: 1   MQLP----VSGFLLPFSLLALIFPVAISGPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 60

Query: 104 PYGRDFVTHTPTGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASG 163
           PYGRDFVTHTPTGRFCNGKLATDFTAE LGFSSYPPAYLSQDATGN+LLTGANFASAASG
Sbjct: 61  PYGRDFVTHTPTGRFCNGKLATDFTAEFLGFSSYPPAYLSQDATGNKLLTGANFASAASG 120

Query: 164 FYDGTAQLYHAISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYY 223
           FYDGTAQLYHA+SLTQQLNYYKEYQ+KVVNMVG  KGN+IFSGAIHLLSAGSSDFIQNYY
Sbjct: 121 FYDGTAQLYHAVSLTQQLNYYKEYQSKVVNMVGTEKGNTIFSGAIHLLSAGSSDFIQNYY 180

Query: 224 VNPLLYRTYSPAQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSN 283
           VNPLLYRTYSP QFSDILITS+S+FIQNLYGLGARRIGVTGLPPLGCLPAAITLF SGSN
Sbjct: 181 VNPLLYRTYSPQQFSDILITSFSSFIQNLYGLGARRIGVTGLPPLGCLPAAITLFGSGSN 240

Query: 284 QCIQRLNQDAIAFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRA 343
           QCIQRLNQDAIAFNTKL+STSQSLQN+FSDLKLV FDIYQPLL+M+SKPAENGFFESRRA
Sbjct: 241 QCIQRLNQDAIAFNTKLQSTSQSLQNRFSDLKLVAFDIYQPLLDMVSKPAENGFFESRRA 300

Query: 344 CCGTGTVETSLLCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           CCGTGTVETS LCNS SIGTCSNATGYVFWDGFHPTE+ANQVLAGDL++QGF+LI
Sbjct: 301 CCGTGTVETSFLCNSRSIGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of MC01g0588 vs. NCBI nr
Match: XP_023517436.1 (GDSL esterase/lipase At5g03820-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 644 bits (1661), Expect = 3.09e-231
Identity = 317/355 (89.30%), Postives = 335/355 (94.37%), Query Frame = 0

Query: 44  MQLPPDAGAAAFWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 103
           MQLP    A  F + FSLLAL+ PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFP
Sbjct: 1   MQLP----AGGFLVTFSLLALIFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 60

Query: 104 PYGRDFVTHTPTGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASG 163
           PYGRDFVTH PTGRFCNGKLATDFTAE LGFSSYPPAYLSQDATGN+LLTGANFASAASG
Sbjct: 61  PYGRDFVTHAPTGRFCNGKLATDFTAEFLGFSSYPPAYLSQDATGNKLLTGANFASAASG 120

Query: 164 FYDGTAQLYHAISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYY 223
           FYDGTAQLYH+ISLTQQLNYYKEYQ+KVVNMVG GKGN+IFSG IHLLSAGSSDFIQNYY
Sbjct: 121 FYDGTAQLYHSISLTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDFIQNYY 180

Query: 224 VNPLLYRTYSPAQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSN 283
           VNPLLYRTYSP QFSDIL+TSYS FIQNLYGLGARRIGVTGLPP+GCLPAAITLF SGSN
Sbjct: 181 VNPLLYRTYSPQQFSDILVTSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGSN 240

Query: 284 QCIQRLNQDAIAFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRA 343
           QCIQRLNQDA+AFNTKL+STSQSLQN+FS LKLVVFDIYQPLLNM+SKPA+NGFFE+RRA
Sbjct: 241 QCIQRLNQDAMAFNTKLQSTSQSLQNRFSGLKLVVFDIYQPLLNMVSKPADNGFFEARRA 300

Query: 344 CCGTGTVETSLLCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           CCGTGTVETSLLCN+GSIGTCSNATGYVFWDGFHPTE+AN+VLA DLISQGF+LI
Sbjct: 301 CCGTGTVETSLLCNNGSIGTCSNATGYVFWDGFHPTEAANKVLAADLISQGFSLI 351

BLAST of MC01g0588 vs. ExPASy TrEMBL
Match: A0A6J1CQX7 (GDSL esterase/lipase At5g03810-like OS=Momordica charantia OX=3673 GN=LOC111013759 PE=3 SV=1)

HSP 1 Score: 800 bits (2067), Expect = 1.18e-292
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MLPEAKFVHKFSPTCPLHLHSLSLLLPLLPKPKGFTILALERKMQLPPDAGAAAFWIPFS 60
           MLPEAKFVHKFSPTCPLHLHSLSLLLPLLPKPKGFTILALERKMQLPPDAGAAAFWIPFS
Sbjct: 1   MLPEAKFVHKFSPTCPLHLHSLSLLLPLLPKPKGFTILALERKMQLPPDAGAAAFWIPFS 60

Query: 61  LLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCN 120
           LLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCN
Sbjct: 61  LLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCN 120

Query: 121 GKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQ 180
           GKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQ
Sbjct: 121 GKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQ 180

Query: 181 LNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI 240
           LNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI
Sbjct: 181 LNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDI 240

Query: 241 LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL 300
           LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL
Sbjct: 241 LITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKL 300

Query: 301 KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS 360
           KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS
Sbjct: 301 KSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGS 360

Query: 361 IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI
Sbjct: 361 IGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398

BLAST of MC01g0588 vs. ExPASy TrEMBL
Match: A0A6J1I5T7 (GDSL esterase/lipase At5g03820-like OS=Cucurbita maxima OX=3661 GN=LOC111471301 PE=3 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 2.59e-232
Identity = 319/355 (89.86%), Postives = 335/355 (94.37%), Query Frame = 0

Query: 44  MQLPPDAGAAAFWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 103
           MQLP    AA F + FSLLALV PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFP
Sbjct: 1   MQLP----AAGFLVTFSLLALVFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 60

Query: 104 PYGRDFVTHTPTGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASG 163
           PYGRDFVTH PTGRFCNGKLATDFTAE LGFSSYPPAYL QDATGN+LLTGANFASAASG
Sbjct: 61  PYGRDFVTHAPTGRFCNGKLATDFTAELLGFSSYPPAYLGQDATGNKLLTGANFASAASG 120

Query: 164 FYDGTAQLYHAISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYY 223
           FYDGTAQLYH+ISLTQQLNYYKEYQ+KVVNMVG GKGN+IFSG IHLLSAGSSDFIQNYY
Sbjct: 121 FYDGTAQLYHSISLTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDFIQNYY 180

Query: 224 VNPLLYRTYSPAQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSN 283
           VNPLLYRTYSP QFSDILITSYS FIQNLYGLGARRIGVTGLPP+GCLPAAITLF SGSN
Sbjct: 181 VNPLLYRTYSPQQFSDILITSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGSN 240

Query: 284 QCIQRLNQDAIAFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRA 343
           QCIQRLNQDA+AFNTKL+STSQSLQN+FSDLKLVVFDIYQPLLNM+SKPA+NGFFE+RRA
Sbjct: 241 QCIQRLNQDAMAFNTKLQSTSQSLQNRFSDLKLVVFDIYQPLLNMVSKPADNGFFEARRA 300

Query: 344 CCGTGTVETSLLCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           CCGTGTVETSLLCN+GSIGTCSN TGYVFWDGFHPTE+AN+VLA DLISQGF+LI
Sbjct: 301 CCGTGTVETSLLCNNGSIGTCSNVTGYVFWDGFHPTEAANKVLAADLISQGFSLI 351

BLAST of MC01g0588 vs. ExPASy TrEMBL
Match: A0A6J1HHX7 (GDSL esterase/lipase At5g03820-like OS=Cucurbita moschata OX=3662 GN=LOC111463132 PE=3 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 3.68e-232
Identity = 318/355 (89.58%), Postives = 336/355 (94.65%), Query Frame = 0

Query: 44  MQLPPDAGAAAFWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 103
           MQLP    A  F + FSLLALV PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFP
Sbjct: 1   MQLP----AGGFLVTFSLLALVFPVAISDPLVPALCIFGDSVVDVGNNNNLLTVVKANFP 60

Query: 104 PYGRDFVTHTPTGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASG 163
           PYGRDFVTH PTGRFCNGKLATDFTAE LGFSSYPPAYLSQDATGN+LLTGANFASAASG
Sbjct: 61  PYGRDFVTHAPTGRFCNGKLATDFTAEFLGFSSYPPAYLSQDATGNKLLTGANFASAASG 120

Query: 164 FYDGTAQLYHAISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYY 223
           FYDGTAQLYH+I+LTQQLNYYKEYQ+KVVNMVG GKGN+IFSG IHLLSAGSSD+IQNYY
Sbjct: 121 FYDGTAQLYHSIALTQQLNYYKEYQSKVVNMVGTGKGNAIFSGGIHLLSAGSSDYIQNYY 180

Query: 224 VNPLLYRTYSPAQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSN 283
           VNPLLYRTYSP QFSDILITSYS FIQNLYGLGARRIGVTGLPP+GCLPAAITLF SGSN
Sbjct: 181 VNPLLYRTYSPQQFSDILITSYSNFIQNLYGLGARRIGVTGLPPMGCLPAAITLFGSGSN 240

Query: 284 QCIQRLNQDAIAFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRA 343
           QCIQRLNQDA+AFNTKL+STSQSLQN+FSDLKLVVFDIYQPLLNM+SKPA+NGFFE+RRA
Sbjct: 241 QCIQRLNQDALAFNTKLQSTSQSLQNRFSDLKLVVFDIYQPLLNMVSKPADNGFFEARRA 300

Query: 344 CCGTGTVETSLLCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           CCGTGTVETSLLCN+GSIGTCSNATGYVFWDGFHPTE+AN+VLA DLISQGF+LI
Sbjct: 301 CCGTGTVETSLLCNNGSIGTCSNATGYVFWDGFHPTEAANKVLASDLISQGFSLI 351

BLAST of MC01g0588 vs. ExPASy TrEMBL
Match: A0A5D3C7V6 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00810 PE=3 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 1.17e-228
Identity = 310/344 (90.12%), Postives = 328/344 (95.35%), Query Frame = 0

Query: 55  FWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTP 114
           F +PF LLAL+ PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTP
Sbjct: 8   FLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTP 67

Query: 115 TGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHA 174
           TGRFCNGKLATDFTAE+LGFSSYPPAYLSQDATGN+LLTGANFASAASGFYDGTAQLYHA
Sbjct: 68  TGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDGTAQLYHA 127

Query: 175 ISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSP 234
           +SLTQQLNYYKEYQ+KVVNMVG  K N+IFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSP
Sbjct: 128 VSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSP 187

Query: 235 AQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAI 294
            QFSDILITS+S FIQNLYG+GARRIGVTGLPPLGCLPAAITLF SGSNQCIQRLNQDAI
Sbjct: 188 QQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSNQCIQRLNQDAI 247

Query: 295 AFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSL 354
           AFNTKL+S + SLQN+FSDLKLV FDIYQPLLNM+SKPAENGFFESRRACCGTGTVETS 
Sbjct: 248 AFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACCGTGTVETSF 307

Query: 355 LCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           LCNS S+GTCSNATGYVFWDGFHPTE+ANQVLAGDL++QGF+LI
Sbjct: 308 LCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of MC01g0588 vs. ExPASy TrEMBL
Match: A0A5A7SKR2 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001620 PE=3 SV=1)

HSP 1 Score: 637 bits (1642), Expect = 1.17e-228
Identity = 310/344 (90.12%), Postives = 328/344 (95.35%), Query Frame = 0

Query: 55  FWIPFSLLALVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTP 114
           F +PF LLAL+ PVA  DPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTP
Sbjct: 8   FLLPFILLALIFPVAITDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTP 67

Query: 115 TGRFCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHA 174
           TGRFCNGKLATDFTAE+LGFSSYPPAYLSQDATGN+LLTGANFASAASGFYDGTAQLYHA
Sbjct: 68  TGRFCNGKLATDFTAENLGFSSYPPAYLSQDATGNKLLTGANFASAASGFYDGTAQLYHA 127

Query: 175 ISLTQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSP 234
           +SLTQQLNYYKEYQ+KVVNMVG  K N+IFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSP
Sbjct: 128 VSLTQQLNYYKEYQSKVVNMVGTEKANAIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSP 187

Query: 235 AQFSDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAI 294
            QFSDILITS+S FIQNLYG+GARRIGVTGLPPLGCLPAAITLF SGSNQCIQRLNQDAI
Sbjct: 188 QQFSDILITSFSNFIQNLYGMGARRIGVTGLPPLGCLPAAITLFGSGSNQCIQRLNQDAI 247

Query: 295 AFNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSL 354
           AFNTKL+S + SLQN+FSDLKLV FDIYQPLLNM+SKPAENGFFESRRACCGTGTVETS 
Sbjct: 248 AFNTKLQSATSSLQNRFSDLKLVAFDIYQPLLNMVSKPAENGFFESRRACCGTGTVETSF 307

Query: 355 LCNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 398
           LCNS S+GTCSNATGYVFWDGFHPTE+ANQVLAGDL++QGF+LI
Sbjct: 308 LCNSRSVGTCSNATGYVFWDGFHPTEAANQVLAGDLLTQGFSLI 351

BLAST of MC01g0588 vs. TAIR 10
Match: AT5G03810.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.1e-137
Identity = 237/332 (71.39%), Postives = 277/332 (83.43%), Query Frame = 0

Query: 68  VATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDF 127
           V TG+ LVPAL I GDSVVD GNNN+ +T+VKANFPPYGRDFV H+ TGRF NGKLATDF
Sbjct: 21  VGTGETLVPALIIMGDSVVDAGNNNHRITLVKANFPPYGRDFVAHSATGRFSNGKLATDF 80

Query: 128 TAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEY 187
           TAE+LGF+SYP AYLSQ+A    LLTGANFAS ASGF D TA  Y+AI+L+QQL  YKEY
Sbjct: 81  TAENLGFTSYPVAYLSQEANETNLLTGANFASGASGFDDATAIFYNAITLSQQLKNYKEY 140

Query: 188 QNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITSYST 247
           QNKV N+VG+ + N IFSGAIHLLS GSSDF+Q+YY+NP+L R ++P Q+SD L+ SYST
Sbjct: 141 QNKVTNIVGKERANEIFSGAIHLLSTGSSDFLQSYYINPILNRIFTPDQYSDHLLRSYST 200

Query: 248 FIQNLYGLGARRIGVTGLPPLGCLPAAITLFCS-GSNQCIQRLNQDAIAFNTKLKSTSQS 307
           F+QNLYGLGARRIGVT LPPLGCLPAAITLF   G+N C++RLNQDA++FNTKL +TS +
Sbjct: 201 FVQNLYGLGARRIGVTTLPPLGCLPAAITLFGGVGNNMCVERLNQDAVSFNTKLNNTSIN 260

Query: 308 LQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGSIGTCSN 367
           L N    LKLVVFDIY PLLNM+  P E GFFESRRACCGTGT+ETS LCN+ S+GTCSN
Sbjct: 261 LTNNLPGLKLVVFDIYNPLLNMVINPVEYGFFESRRACCGTGTMETSFLCNALSVGTCSN 320

Query: 368 ATGYVFWDGFHPTESANQVLAGDLISQGFALI 399
           AT YVFWDGFHP+E+AN+V+A +L+ QG  LI
Sbjct: 321 ATNYVFWDGFHPSEAANRVIANNLLVQGIPLI 352

BLAST of MC01g0588 vs. TAIR 10
Match: AT5G03820.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 485.3 bits (1248), Expect = 4.6e-137
Identity = 239/343 (69.68%), Postives = 277/343 (80.76%), Query Frame = 0

Query: 59  FSLLA-LVIPVATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGR 118
           FS++A     V TG+PLVPAL I GDSVVD GNNN L T++KANFPPYGRDF+ H  TGR
Sbjct: 11  FSVIACFYAGVGTGEPLVPALIIMGDSVVDAGNNNRLNTLIKANFPPYGRDFLAHNATGR 70

Query: 119 FCNGKLATDFTAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISL 178
           F NGKLATDFTAESLGF+SYP  YLSQ+A G  LLTGANFAS ASG+ DGTA  Y+AI+L
Sbjct: 71  FSNGKLATDFTAESLGFTSYPVPYLSQEANGTNLLTGANFASGASGYDDGTAIFYNAITL 130

Query: 179 TQQLNYYKEYQNKVVNMVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQF 238
            QQL  YKEYQNKV N+VG  + N IFSGAIHLLS GSSDF+Q+YY+NP+L R ++P Q+
Sbjct: 131 NQQLKNYKEYQNKVTNIVGSERANKIFSGAIHLLSTGSSDFLQSYYINPILNRIFTPDQY 190

Query: 239 SDILITSYSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLF--CSGSNQCIQRLNQDAIA 298
           SD L+  YSTF+QNLY LGAR+IGVT LPPLGCLPAAITLF     +N C++RLNQDA++
Sbjct: 191 SDRLMKPYSTFVQNLYDLGARKIGVTTLPPLGCLPAAITLFGETGNNNTCVERLNQDAVS 250

Query: 299 FNTKLKSTSQSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLL 358
           FNTKL +TS +L N    LKLVVFDIY PLLNM   P ENGFFESRRACCGTGTVETS L
Sbjct: 251 FNTKLNNTSMNLTNNLPGLKLVVFDIYNPLLNMAMNPVENGFFESRRACCGTGTVETSFL 310

Query: 359 CNSGSIGTCSNATGYVFWDGFHPTESANQVLAGDLISQGFALI 399
           CN+ S+GTCSNAT YVFWDGFHP+E+AN+V+A +L+ QG  LI
Sbjct: 311 CNARSVGTCSNATNYVFWDGFHPSEAANRVIANNLLVQGIPLI 353

BLAST of MC01g0588 vs. TAIR 10
Match: AT3G53100.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 461.8 bits (1187), Expect = 5.4e-130
Identity = 214/325 (65.85%), Postives = 270/325 (83.08%), Query Frame = 0

Query: 74  LVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDFTAESLG 133
           +VPAL +FGDS+VDVGNNNNLL++VK+NF PYGRDF+   PTGRFCNGKLA DF+AE LG
Sbjct: 26  VVPALIMFGDSIVDVGNNNNLLSIVKSNFLPYGRDFIDQRPTGRFCNGKLAVDFSAEYLG 85

Query: 134 FSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEYQNKVVN 193
           FSSYPPA+LS++A+   +L GANFASA+SG+YD T+  + +ISLT+QL+YY+ YQN+V  
Sbjct: 86  FSSYPPAFLSREASNENILIGANFASASSGYYDATSVPFGSISLTRQLSYYRAYQNRVTR 145

Query: 194 MVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITSYSTFIQNLY 253
           M+GRG    +FS  IH+LSAGSSDF+QNYY+NPLL    +P QF+DIL+ S+S FIQNLY
Sbjct: 146 MIGRGNARILFSRGIHILSAGSSDFLQNYYINPLLNILNTPDQFADILLRSFSEFIQNLY 205

Query: 254 GLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKLKSTSQSLQNKFSD 313
            LGARRIGV  LPP+GCLPAAITLF +G+  C++RLN DAI FNTKL++T++ L N+ S 
Sbjct: 206 ELGARRIGVISLPPMGCLPAAITLFGAGNKSCVERLNNDAIMFNTKLENTTRLLMNRHSG 265

Query: 314 LKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGSIGTCSNATGYVFW 373
           L+LV F++YQP L++I+ P +NGFFE++RACCGTGT+ETS LCNS S GTC NATGYVFW
Sbjct: 266 LRLVAFNVYQPFLDIITNPTDNGFFETKRACCGTGTIETSFLCNSLSFGTCVNATGYVFW 325

Query: 374 DGFHPTESANQVLAGDLISQGFALI 399
           DGFHPTE+ N++LAG L+ QG +LI
Sbjct: 326 DGFHPTEAVNELLAGQLLGQGISLI 350

BLAST of MC01g0588 vs. TAIR 10
Match: AT5G22810.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 455.7 bits (1171), Expect = 3.9e-128
Identity = 221/334 (66.17%), Postives = 266/334 (79.64%), Query Frame = 0

Query: 68  VATGDPLVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDF 127
           V    PLVPA+ IFGDSVVDVGNNN++ T+VKANFPPYGRDF THTPTGRFCNGKLATDF
Sbjct: 3   VMKAQPLVPAIFIFGDSVVDVGNNNDIYTIVKANFPPYGRDFTTHTPTGRFCNGKLATDF 62

Query: 128 TAESLGFSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEY 187
           TAE+LGF SYP AYLS+ A G  LL GANFASAASG+YDGTA+LY AISL QQL +YK+Y
Sbjct: 63  TAENLGFKSYPQAYLSKKAKGKNLLIGANFASAASGYYDGTAKLYSAISLPQQLEHYKDY 122

Query: 188 QNKVVNMV---GRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITS 247
            +++  +         ++I S  I+++SAGSSDFIQNYY+NPLLYR  SP +FSD+LI S
Sbjct: 123 ISRIQEIATSNNNSNASAIISNGIYIVSAGSSDFIQNYYINPLLYRDQSPDEFSDLLILS 182

Query: 248 YSTFIQNLYGLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKLKSTS 307
           YS+FIQNLY LGARRIGVT LPPLGCLPAAIT+       C ++LN DAI+FN KL +TS
Sbjct: 183 YSSFIQNLYSLGARRIGVTTLPPLGCLPAAITVVGPHEGGCSEKLNNDAISFNNKLNTTS 242

Query: 308 QSLQNKFSDLKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVETSLLCNSGSIGTC 367
           Q L+     L LVVFDIYQPL ++ ++P+E GF E+RRACCGTG +ETS+LCN  S+GTC
Sbjct: 243 QDLKRNLIGLNLVVFDIYQPLYDLATRPSEFGFAEARRACCGTGLLETSILCNPKSVGTC 302

Query: 368 SNATGYVFWDGFHPTESANQVLAGDLISQGFALI 399
           +NAT YVFWDGFHPTE+AN++LA +L+  G +LI
Sbjct: 303 NNATEYVFWDGFHPTEAANKILADNLLVSGISLI 336

BLAST of MC01g0588 vs. TAIR 10
Match: AT3G16370.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 414.1 bits (1063), Expect = 1.3e-115
Identity = 201/326 (61.66%), Postives = 249/326 (76.38%), Query Frame = 0

Query: 74  LVPALCIFGDSVVDVGNNNNLLTVVKANFPPYGRDFVTHTPTGRFCNGKLATDFTAESLG 133
           LVPA+  FGDSVVDVGNNN L T+ +A++PPYGRDF  H  TGRFCNGKLATD TAE+LG
Sbjct: 27  LVPAIMTFGDSVVDVGNNNYLPTLFRADYPPYGRDFANHKATGRFCNGKLATDITAETLG 86

Query: 134 FSSYPPAYLSQDATGNRLLTGANFASAASGFYDGTAQLYHAISLTQQLNYYKEYQNKVVN 193
           F+ YPPAYLS +A+G  LL GANFASAASG+ D  A L HAI L QQ+ Y+KEY++K++ 
Sbjct: 87  FTKYPPAYLSPEASGKNLLIGANFASAASGYDDKAALLNHAIPLYQQVEYFKEYKSKLIK 146

Query: 194 MVGRGKGNSIFSGAIHLLSAGSSDFIQNYYVNPLLYRTYSPAQFSDILITSYSTFIQNLY 253
           + G  K +SI  GAI LLSAGSSDF+QNYYVNPLLY+ Y+   +   LI ++STFI+ +Y
Sbjct: 147 IAGSKKADSIIKGAICLLSAGSSDFVQNYYVNPLLYKVYTVDAYGSFLIDNFSTFIKQVY 206

Query: 254 GLGARRIGVTGLPPLGCLPAAITLFCSGSNQCIQRLNQDAIAFNTKLKSTSQSLQNKFSD 313
            +GAR+IGVT LPP GCLPAA TLF      C+ RLN DA  FN KL + +  LQ ++SD
Sbjct: 207 AVGARKIGVTSLPPTGCLPAARTLFGFHEKGCVSRLNTDAQNFNKKLNAAASKLQKQYSD 266

Query: 314 LKLVVFDIYQPLLNMISKPAENGFFESRRACCGTGTVE-TSLLCNSGSIGTCSNATGYVF 373
           LK+VVFDIY PL +++  P+++GF E+ + CCGTGTVE TSLLCN  S GTCSNAT YVF
Sbjct: 267 LKIVVFDIYSPLYDLVQNPSKSGFTEATKGCCGTGTVETTSLLCNPKSFGTCSNATQYVF 326

Query: 374 WDGFHPTESANQVLAGDLISQGFALI 399
           WD  HP+E+AN++LA  LI QGF+L+
Sbjct: 327 WDSVHPSEAANEILATALIGQGFSLL 352