MC01g0539 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0539
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionOTU domain-containing protein
LocationMC01: 11801091 .. 11804810 (+)
RNA-Seq ExpressionMC01g0539
SyntenyMC01g0539
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCATGAACGATTATGATTCCGATGTCATTAGGTGGGGTCTTGGTTTTTTCAATGGGGATTCATCGTATAGTACCGAATACTTCTCTGACATGGCACAACATGATGTGAATTTTTATGAGCATTATTATGGGAACCATTGCAATAACGAGGTTAACAATGTGGAGAACGATGAGATTATTGCGCACACTCTTCAGGAGGAGTTTTCCCGATTAGAAATTGCAGAGGCCTCAGGCTATTCAAATGTAGAAGAAGAGCATTCCCGAGCATCTAATTTTGCACTTGATTGGAACAATCCCTCCTCAAGAAACTATGGTTCAGGTTCTCACTAATACGAGCCACTTTCTTCTTTCTCCGTATATGTGTGATATTGATCCTTACACGCGCCTATAAAATTTTAATAATCTGCAGAGAGTGATTCTGTTGAAGATGAAGTTGAAACTGGGGAGCTTTCTAGTTCATGTTCTAGTCCAGGTCATGACGAAAATTCCTATCCTTATGGGAATGGTGATCAAGTAGATTGGAGATTGGATCAAATGATTACGGCACCTGTGAGATTTTCTATAATTACATCTGTTTCTCCTATAGATTTATTGTGGAGATAATATTCAACCTTCTATGCTGGTAAATTTGTGCACGACATTTTATGAAGCATGCTTTTTCGCAGGGAATTCTTACTGCTGACATCTTGGAATTATCTATATCTTATTAGAATTAGGAATTGTTTTTCTCATGATAGGGAAATATTTTGTAATGATGTGCGTAGATTCTTTTTTTTTTTTTTGACAGCTGGAGGATTTTGAACTATCCTTGCATCATCCATGCATATGTTTTGTCAGTTTGGCACGGCCACTAGAATTTTAGGGTTTTACATTCTGTAGTTGATTTCTCTTGGAACCATTGACCTAGTCATGCCCGCCACAATCTATTCTCAACATGCTAATTTTTTGTTCTCACTGTTTATTTATTTGTTTGAAGAAACAAAGAATCTTTAGTAGCTATATGGTTTCAGAGGCTAATAACTAGTTCTTTCCAATTTATGTGCTTTATATACATATGTGGGACCCATGATTGTTCGTCTGGCTACTACAAAGTTGCCTTAAAAATTTTCTTGTGATTTGTGCAGCATGTTCCAAGAATTAATGGTGAGATTCCTTCGATTGATGAAGTAACTTCAGATCACCAAAGGTTGCTGGATAGGTAATATCTCAAAAGCTCTCTGTTGTCTGATGTGGTTGAATTTGTAGTCTATTGTTTATAGCCAGCTTGTGTTCGAGTTTCTTTAACTTTTTTTCAGCTTGCTATTTGATGGTCTTTTAGAATATGATTGTTGTTTGACATTGAATTTTGGGTTTTAATTGTCATGACCGTCCTCTGTCTTCTCAATCTTAGGTTGAAAGTATATGATTTTGTCGAACGAAAGGTTAAAGGAGATGGTAATTGTCAGGTCAGTTGTTTCTCAGGCATGTGATTCTGGTCACAATTGAACAGTTTGGATTGAACATGACATACTCATATTAGTTTCCAATTCTCAGTTCCGTGCTTTGTCAGATCAACTATTTGGAACATCTAAACGCCACAGGTTGGTGAGACAAAGTGTTGTGAATCAGGTTTGTCATTGTTGCTTATAGCTTTATGCTTGTAGTTATTCTTCAGGTATACTGGATTATAACCTATTTATCATGGATTTCTCTCTCCCCTTTTTTTTCCACCAGCTCAAGTCGCATCGGGAGATTTATGAGGGATATGTTCCCATGGGATATGATGATTATTTGGAAAAGATGATCATGTATCACTTGAACTTCTATTTATTTCATTCTTTTCAGCCCCATACTCTAGTTCTGTTTGGATAATTCTTGTTACACTTCATTTGAAATGTTTCTAGGAGCGGCGAGTGGGGTGATCACGTGACACTACAGGCAGCTGCCGATTGGGTATGGTCATTGCAAGAAATTGTCTCCGGCTTCTAAGTGTTTTCTTTAGATAGTGTTGAAAGTTTAAAATGTGATTGTTGTTCTTTCATGAAGTTTATCCAAATCAACCATCATGGTGTGACCTAGTGATCAATAAGGGCCAATATAAATAGCAAAGGGCTTAGAGAGAATGAGTTCGAATCATGGTAGCCACCTATCTAGATTTACTTTGGCAACCAAATGTAGTAGGGTCAAACGGTCCCGTGAGATTAGTCGAAATGTGCGCAAGCTGGCCCTGACACTCACGGGTATTAAAAAAAAAAGAACGTTACCCAAATCTTTAAATAATTTTCTACTATGTTAATCTTTAGAATGACTAAAAAAAAGGAAGTAAACTCGTGTAAGAGATTTGAAAGTTGTAGGTTCGACCATTTTTGCGTATTTTTACCTATCTGGATTATACTTTTTACTGTGTTAGATGTTCCATTGTGTCAATGGGCGTCGGAACAATGTCTACCTGTGCTGCCATTTACTTGAATGAAGTTGCTAGATTGATATGAAATTTAAAAAAAAAATCCATATCCTAATTATATCAAATAATAACCTTGATAACTCCTTCCCCGATCACCAATCCCCTGACTATGTTCCTCCCCCACGTCCTCCTCCCTCTTTTCTGCTTTATTGTCTTTAGAAATTTGTCATTTATGAATCTAAATGTGTCAATGACTGTTTTATGTTGCAGTATGGCGTTAAAATATTTGTTATGACTTCTTTCAAGGAAAACTGTTGTATAGAGATTCTCCCTAGTTTCCAGAAGAAAAAACAAGGTAAATTCTTAACTCAATACTTTTGTATCAACTTGAGTAACTTTTCTGGTTCCTTTACCTGGTTATGGGCCTGTAATTGACCCAATATTTTGCTGTTTTACTTGGAAAATGGCCATATTTAGTGCATTGCAACTTTTTTTTTTTTTTTCTAAAATTGCTTTTAACCTCTCACCAGCAGCACTTTACTTTCTCATTTTTTTCTCCTATTAATTGAGTAAATTTAACTGGAAGAACTAAGAAGATGGTCTGCGAAAGTTTATTCAACTGTGCTGTTCTTTCCCGTCCGCAGAATTCATGCCCTGTTTTCCATTTCTTTTTTGTCTCATTTGTTGTATCCGGGGTGCTGTCAGACCATTATATCTTTCCAACAAAACAAAACCCATCTCTTTCGTACAAAGGAGGCAATTCCAATATTCACCTTATTGACTTCCCTCATTTTGTAATCCACCATTCTTTATTATTTTTTTTTATTAATCAATAGAACGTTGAACCAATAATACATGTATGTAATTTCGTTTCCAGAGAGCTTCACTTTTTACTCCTTTGTGCAATATGCAGTAATTTTTCTAAGCTTTTGGGCAGAGGTGCATTACAACTCAATCTATCCTCAAGGAGGTAACTATTTTACTCTTCTATCCCTGGTGAACTTGAAGCTGTCTTATATCATAACTTACAAATTCTCACTCACGTTTTTTAGTCAGAAAAGTAACATATAGAAGGTTCATTTTTTTTTTTATCTGCACTAAGATCCGAACAATTCGCTTGATTGAGCATGAATATTGATTCTGTAGTTTTAACTCTCCAATTTTAATGGTCCAGTCTTCACTTTTGATGTCTTTGACGTTTTGTGCTTGGGAACACTAGCTGCTAACTATTTATGTCAAAACTCCATATTCATTTAGATGTTTCCTCAGTTGCAGATGAGCAATCAAACGATTCTAGAAAGAGGAGGAAATGGTGGATTTTCGGGAATAAGCAT

mRNA sequence

ATGGTCATGAACGATTATGATTCCGATGTCATTAGGTGGGGTCTTGGTTTTTTCAATGGGGATTCATCGTATAGTACCGAATACTTCTCTGACATGGCACAACATGATGTGAATTTTTATGAGCATTATTATGGGAACCATTGCAATAACGAGGTTAACAATGTGGAGAACGATGAGATTATTGCGCACACTCTTCAGGAGGAGTTTTCCCGATTAGAAATTGCAGAGGCCTCAGGCTATTCAAATGTAGAAGAAGAGCATTCCCGAGCATCTAATTTTGCACTTGATTGGAACAATCCCTCCTCAAGAAACTATGGTTCAGAGAGTGATTCTGTTGAAGATGAAGTTGAAACTGGGGAGCTTTCTAGTTCATGTTCTAGTCCAGGTCATGACGAAAATTCCTATCCTTATGGGAATGGTGATCAAGTAGATTGGAGATTGGATCAAATGATTACGGCACCTCATGTTCCAAGAATTAATGGTGAGATTCCTTCGATTGATGAAGTAACTTCAGATCACCAAAGGTTGCTGGATAGGTTGAAAGTATATGATTTTGTCGAACGAAAGGTTAAAGGAGATGGTAATTGTCAGTTCCGTGCTTTGTCAGATCAACTATTTGGAACATCTAAACGCCACAGGTTGGTGAGACAAAGTGTTGTGAATCAGCTCAAGTCGCATCGGGAGATTTATGAGGGATATGTTCCCATGGGATATGATGATTATTTGGAAAAGATGATCATGAGCGGCGAGTGGGGTGATCACGTGACACTACAGGCAGCTGCCGATTGGTATGGCGTTAAAATATTTGTTATGACTTCTTTCAAGGAAAACTGTTGTATAGAGATTCTCCCTAGTTTCCAGAAGAAAAAACAAGTAATTTTTCTAAGCTTTTGGGCAGAGGTGCATTACAACTCAATCTATCCTCAAGGAGATGTTTCCTCAGTTGCAGATGAGCAATCAAACGATTCTAGAAAGAGGAGGAAATGGTGGATTTTCGGGAATAAGCAT

Coding sequence (CDS)

ATGGTCATGAACGATTATGATTCCGATGTCATTAGGTGGGGTCTTGGTTTTTTCAATGGGGATTCATCGTATAGTACCGAATACTTCTCTGACATGGCACAACATGATGTGAATTTTTATGAGCATTATTATGGGAACCATTGCAATAACGAGGTTAACAATGTGGAGAACGATGAGATTATTGCGCACACTCTTCAGGAGGAGTTTTCCCGATTAGAAATTGCAGAGGCCTCAGGCTATTCAAATGTAGAAGAAGAGCATTCCCGAGCATCTAATTTTGCACTTGATTGGAACAATCCCTCCTCAAGAAACTATGGTTCAGAGAGTGATTCTGTTGAAGATGAAGTTGAAACTGGGGAGCTTTCTAGTTCATGTTCTAGTCCAGGTCATGACGAAAATTCCTATCCTTATGGGAATGGTGATCAAGTAGATTGGAGATTGGATCAAATGATTACGGCACCTCATGTTCCAAGAATTAATGGTGAGATTCCTTCGATTGATGAAGTAACTTCAGATCACCAAAGGTTGCTGGATAGGTTGAAAGTATATGATTTTGTCGAACGAAAGGTTAAAGGAGATGGTAATTGTCAGTTCCGTGCTTTGTCAGATCAACTATTTGGAACATCTAAACGCCACAGGTTGGTGAGACAAAGTGTTGTGAATCAGCTCAAGTCGCATCGGGAGATTTATGAGGGATATGTTCCCATGGGATATGATGATTATTTGGAAAAGATGATCATGAGCGGCGAGTGGGGTGATCACGTGACACTACAGGCAGCTGCCGATTGGTATGGCGTTAAAATATTTGTTATGACTTCTTTCAAGGAAAACTGTTGTATAGAGATTCTCCCTAGTTTCCAGAAGAAAAAACAAGTAATTTTTCTAAGCTTTTGGGCAGAGGTGCATTACAACTCAATCTATCCTCAAGGAGATGTTTCCTCAGTTGCAGATGAGCAATCAAACGATTCTAGAAAGAGGAGGAAATGGTGGATTTTCGGGAATAAGCAT

Protein sequence

MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIFGNKH
Homology
BLAST of MC01g0539 vs. ExPASy Swiss-Prot
Match: Q9LZF7 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10 OS=Arabidopsis thaliana OX=3702 GN=OTU10 PE=1 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 4.5e-74
Identity = 166/363 (45.73%), Postives = 229/363 (63.09%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGD-SSYSTEYFSDMAQHDVNFYEH------YYGNHCNNEVN 60
           MV ++ ++ ++ W LG        Y  E   +  +  V  + H      YY  + ++  +
Sbjct: 1   MVSHEENTSIVEWFLGPHPYTYPPYGIEMIHEDDEVAVAHHHHHHQSGEYYREYEDHRSS 60

Query: 61  NVENDEIIAHTLQEEFSRLEIAEASGYS--NVEEEHSRA--------SNFALDWNNPS-S 120
           +V+NDEIIA TLQ++F +LEIAE++ YS  N +++H +         +N    WN+ S +
Sbjct: 61  DVDNDEIIARTLQDDFLQLEIAESNDYSHQNQQQQHQQEGYTNNYSNNNNGYAWNDQSPA 120

Query: 121 RNYGSE---SDSVEDEVETGELS-SSCSSPGHDENSYPYG-NGDQVD------WRLDQMI 180
            +Y SE   +D+ +D      ++  SCSSP  D + Y Y    DQ D       RL+QM+
Sbjct: 121 VDYSSEWIGNDNDQDGRSDDSVNVFSCSSPS-DTDEYVYSWESDQCDADGEFGRRLNQMV 180

Query: 181 TAPHVPRINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKR 240
             P++P+INGEIP  +E  SDH+RL +RL+++DF E KV GDGNCQFRAL+DQL+ T+ R
Sbjct: 181 PIPYIPKINGEIPPEEEAVSDHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKTADR 240

Query: 241 HRLVRQSVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVM 300
           H+ VR+ +V QLKS  + Y+GYVPM + DYL KM  SGEWGDHVTLQAAAD Y VKI V+
Sbjct: 241 HKHVRRQIVKQLKSRPDSYQGYVPMDFSDYLRKMSRSGEWGDHVTLQAAADAYRVKIVVL 300

Query: 301 TSFKENCCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWI 335
           TSFK+ C IEILP+ Q+ K VIFLSFWAEVHYN+IY   D S        + +++RKWW 
Sbjct: 301 TSFKDTCYIEILPTSQESKGVIFLSFWAEVHYNAIYLNRDTS------ETELQRKRKWWR 356

BLAST of MC01g0539 vs. ExPASy Swiss-Prot
Match: Q8LBW2 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 9 OS=Arabidopsis thaliana OX=3702 GN=OTU9 PE=1 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.4e-70
Identity = 163/355 (45.92%), Postives = 212/355 (59.72%), Query Frame = 0

Query: 7   DSDVIRWGLGFFN-------GDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNN-VEND 66
           D D +RWGL           G  S  T Y S          E Y     N  V   V+ND
Sbjct: 6   DPDALRWGLHDLEVCTLTNAGSCSSVTRYESGGGGTQGYVREGY-----NQPVTGYVDND 65

Query: 67  EIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNP--SSRNYG------SESD 126
            +IA   Q+E SR+  AEASG +++    S  S  A DW +P     N G       ESD
Sbjct: 66  AVIAQFYQDELSRVARAEASGINSL----SPTSVVAQDWPHPHQGQENQGEAIDITQESD 125

Query: 127 -------SVEDE-VETGELSSSCSSPGHDENSYPYGNGDQVDW-----RLDQMITAPHVP 186
                  ++ED+ V         SSP  D++S      ++  W     RL+QMI   HVP
Sbjct: 126 ILHNHNGNMEDKNVARIRFEGGQSSPSRDDDSVCSVEIEEESWSEVGKRLNQMIPIAHVP 185

Query: 187 RINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQ 246
           +INGE+PS DE  SDH+RL  RL++Y  VE K++GDGNCQFR+LSDQL+ + + H  VR+
Sbjct: 186 KINGELPSEDEQISDHERLFQRLQLYGLVENKIEGDGNCQFRSLSDQLYRSPEHHNFVRE 245

Query: 247 SVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKEN 306
            VVNQL  +REIYEGYVPM Y+DYL+ M  +GEWGDHVTLQAAAD +GV++FV+TSFK+ 
Sbjct: 246 QVVNQLAYNREIYEGYVPMAYNDYLKAMKRNGEWGDHVTLQAAADLFGVRMFVITSFKDT 305

Query: 307 CCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIF 333
           C IEILP FQK  ++I LSFWAEVHYNSIYP+G++         + +K++K+W+F
Sbjct: 306 CYIEILPHFQKSNRLICLSFWAEVHYNSIYPEGEL------PIPEGKKKKKYWVF 345

BLAST of MC01g0539 vs. ExPASy Swiss-Prot
Match: Q9SGA5 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 OS=Arabidopsis thaliana OX=3702 GN=OTU12 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.9e-52
Identity = 109/235 (46.38%), Postives = 147/235 (62.55%), Query Frame = 0

Query: 98  NNPSSRNYGSESDSVEDEVETGELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVP 157
           ++ SS ++ S+ D+ +D +    LS   S                V  RL  +   PHVP
Sbjct: 3   DSSSSTSWSSKKDTEDDRMIAFMLSEEYSK-----------LDGAVGRRLSNLAPVPHVP 62

Query: 158 RINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQ 217
           RIN  IP++++ T DHQRLL RL VY   E KV GDGNCQFRALSDQL+ + + H+ VR+
Sbjct: 63  RINCYIPNLNDATLDHQRLLQRLNVYGLCELKVSGDGNCQFRALSDQLYRSPEYHKQVRR 122

Query: 218 SVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKEN 277
            VV QLK  R +YE YVPM Y  Y +KM   GEWGDH+TLQAAAD +  KI ++TSF++ 
Sbjct: 123 EVVKQLKECRSMYESYVPMKYKRYYKKMGKFGEWGDHITLQAAADRFAAKICLLTSFRDT 182

Query: 278 CCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIF 333
           C IEI+P +Q  K V++LSFW+EVHYNS+Y   D+ +   +     + +RK W+F
Sbjct: 183 CFIEIIPQYQAPKGVLWLSFWSEVHYNSLY---DIQAAPVQH----KPKRKHWLF 219

BLAST of MC01g0539 vs. ExPASy Swiss-Prot
Match: Q0V869 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 11 OS=Arabidopsis thaliana OX=3702 GN=OTU11 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.0e-46
Identity = 109/251 (43.43%), Postives = 148/251 (58.96%), Query Frame = 0

Query: 82  NVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGELSSSCSSPGHDENSYPYGNGD 141
           N     + AS  A    + S+ +  S S SV D   T +  +       DE+    G   
Sbjct: 4   NHRNPFANASTSARASGSTSASSNSSFSSSVAD---TDDDQTIARILAEDESLRREG--- 63

Query: 142 QVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRAL 201
           ++  RL  + + PH PR+N EIP I++ T DH+ L  RL  Y   E +++GDGNCQFRAL
Sbjct: 64  KLGKRLSHLDSIPHTPRVNREIPDINDATLDHELLSGRLATYGLAELQMEGDGNCQFRAL 123

Query: 202 SDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAA 261
           +DQLF  +  H+ VR+ VV QLK  R++YE YVPM Y  Y  KM   GEWGDHVTLQAAA
Sbjct: 124 ADQLFRNADYHKHVRKHVVKQLKQQRKLYEEYVPMKYRHYTRKMKKHGEWGDHVTLQAAA 183

Query: 262 DWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSN 321
           D +  KI ++TSF++   IEILP  +   +  +LSFW+EVHYNS+Y  G V ++ D  + 
Sbjct: 184 DRFEAKICLVTSFRDQSYIEILPHNKNPLREAWLSFWSEVHYNSLYANG-VLALPDVPTR 243

Query: 322 DSRKRRKWWIF 333
             + RRK W+F
Sbjct: 244 --KPRRKHWLF 245

BLAST of MC01g0539 vs. ExPASy Swiss-Prot
Match: Q54P70 (OTU domain-containing protein DDB_G0284757 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0284757 PE=4 SV=2)

HSP 1 Score: 107.5 bits (267), Expect = 3.1e-22
Identity = 61/157 (38.85%), Postives = 91/157 (57.96%), Query Frame = 0

Query: 163 IPSIDEVTSDHQRLLDRLKVYDFVERK-VKGDGNCQFRALSDQLFGTSKRHRLVRQSVVN 222
           +P   EV    QRL +RL++Y     K + GDGNCQ  ALSDQL+G     + VR+++V+
Sbjct: 608 LPQSKEVQIAQQRLNERLELYMLKNSKEIPGDGNCQMHALSDQLYGDLSHSQEVRKTIVD 667

Query: 223 QLKSHRE--------IYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTS 282
            L+ +++        I +      +DDY   M  +G WGDH+TL AAA+ +G KI +++S
Sbjct: 668 WLRKNKDFQLPNGATICQFVNTNNWDDYCNDMSKNGNWGDHLTLLAAAEHFGSKISIISS 727

Query: 283 F--KENCCIEILPSFQKKKQVIFLSFWAEVHYNSIYP 309
              + N  IEI+PS     +V+ LS +AE HY S+ P
Sbjct: 728 VESQSNFFIEIIPSKILNDKVLLLSHYAEFHYGSLCP 764

BLAST of MC01g0539 vs. NCBI nr
Match: XP_022143971.1 (RNA polymerase sigma factor sigF, chloroplastic [Momordica charantia])

HSP 1 Score: 636 bits (1641), Expect = 5.85e-222
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60
           MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI
Sbjct: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60

Query: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120
           IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE
Sbjct: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120

Query: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180
           LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL
Sbjct: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180

Query: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240
           KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD
Sbjct: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240

Query: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300
           YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE
Sbjct: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300

Query: 301 VHYNSIYPQG 310
           VHYNSIYPQG
Sbjct: 301 VHYNSIYPQG 310

BLAST of MC01g0539 vs. NCBI nr
Match: KAG6604409.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 594 bits (1531), Expect = 9.29e-213
Identity = 286/336 (85.12%), Postives = 312/336 (92.86%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60
           MVMNDYDSD I WGL  FNGDSSYS+EYF DMAQHDVNFYEHYYGNHCNNEVN+VENDEI
Sbjct: 1   MVMNDYDSDAISWGLRLFNGDSSYSSEYFPDMAQHDVNFYEHYYGNHCNNEVNHVENDEI 60

Query: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120
           IAHTLQEEFS+LEIAEASGYSN+E+  S+AS + L W+NPSSRNYGSE+DS+ED VET E
Sbjct: 61  IAHTLQEEFSQLEIAEASGYSNIEDP-SQASKYELGWDNPSSRNYGSENDSIEDVVETVE 120

Query: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180
            SSSCSSPG +++ YP+G+  QVDW++DQ+I APHVPRINGEIPSIDEVTSDHQRLLDRL
Sbjct: 121 ASSSCSSPGQEDSYYPHGHDSQVDWKMDQVIPAPHVPRINGEIPSIDEVTSDHQRLLDRL 180

Query: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240
           KVYDFVERKVKGDGNCQFRALSDQLFGTS RH+LVR++VVNQLKSHREIYEGYVPM YDD
Sbjct: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSNRHKLVRENVVNQLKSHREIYEGYVPMAYDD 240

Query: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300
           YLEKM M+GEWGDHVTLQAAADWYGVKIFVMTSFKE CCIEILPSFQKK+QVIFLSFWAE
Sbjct: 241 YLEKMSMTGEWGDHVTLQAAADWYGVKIFVMTSFKETCCIEILPSFQKKRQVIFLSFWAE 300

Query: 301 VHYNSIYPQGDVSSVADEQSNDSRKRRKWWIFGNKH 336
           VHYNSIYPQ D SSVADEQSND+RKRR+WWIFG+KH
Sbjct: 301 VHYNSIYPQEDGSSVADEQSNDNRKRRRWWIFGHKH 335

BLAST of MC01g0539 vs. NCBI nr
Match: XP_031742500.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 isoform X1 [Cucumis sativus] >XP_031742501.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 isoform X1 [Cucumis sativus] >XP_031742502.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 isoform X1 [Cucumis sativus] >KAE8647402.1 hypothetical protein Csa_003892 [Cucumis sativus])

HSP 1 Score: 594 bits (1531), Expect = 1.30e-212
Identity = 298/345 (86.38%), Postives = 313/345 (90.72%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSS--------YSTEYFSDMAQHDVNFYEHYYGNHCNNEV 60
           MVMNDYDSD IRWGL FFNGDS+        YSTEY  D AQHDVNFYEHYYGN+ NNEV
Sbjct: 1   MVMNDYDSDAIRWGLRFFNGDSNGDSNGDMPYSTEYLPDTAQHDVNFYEHYYGNNYNNEV 60

Query: 61  NNVENDEIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSV 120
           NNVENDEIIAHTLQEEFS+LEIAEASGYSNVE+  S+AS FAL WNNPSSRNY SE+DSV
Sbjct: 61  NNVENDEIIAHTLQEEFSQLEIAEASGYSNVEDP-SQASKFALGWNNPSSRNYSSENDSV 120

Query: 121 EDEVETGELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSD 180
           EDEVET E SSSCSSPG  +N YPYG+ +QVDWR+DQMI APHVPRINGEIPSIDEVTSD
Sbjct: 121 EDEVETVEPSSSCSSPGQGDNYYPYGHDNQVDWRMDQMIPAPHVPRINGEIPSIDEVTSD 180

Query: 181 HQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEG 240
           HQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTS RH+LVR++VV+QLKSHREIYEG
Sbjct: 181 HQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSDRHKLVRENVVSQLKSHREIYEG 240

Query: 241 YVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQV 300
           YVPM YDDYLEKM MSGEWGDHVTLQAAADWYGVKIFVMTSFKE CCIEILP+FQKKKQV
Sbjct: 241 YVPMPYDDYLEKMSMSGEWGDHVTLQAAADWYGVKIFVMTSFKETCCIEILPNFQKKKQV 300

Query: 301 IFLSFWAEVHYNSIYPQGDVSSVADEQS-NDSRKRRKWWIFGNKH 336
           IFLSFWAEVHYNSIYPQG VSSVADEQS NDSRKRRKWWIFGNKH
Sbjct: 301 IFLSFWAEVHYNSIYPQGGVSSVADEQSSNDSRKRRKWWIFGNKH 344

BLAST of MC01g0539 vs. NCBI nr
Match: XP_038882896.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10-like isoform X1 [Benincasa hispida] >XP_038882897.1 OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10-like isoform X1 [Benincasa hispida])

HSP 1 Score: 591 bits (1524), Expect = 1.17e-211
Identity = 290/338 (85.80%), Postives = 311/338 (92.01%), Query Frame = 0

Query: 3   MNDYDSDVIRWGLGFFNGDS----SYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVEND 62
           MNDYDSD IRWGL FFNGDS    SYSTEYF DMAQHDVNFYEHYYGNH NNEVN+VEND
Sbjct: 1   MNDYDSDAIRWGLRFFNGDSNGDLSYSTEYFPDMAQHDVNFYEHYYGNHSNNEVNHVEND 60

Query: 63  EIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVET 122
           EIIA TLQEEFS+LE+AEASGYSNVE+  S+AS FAL WNNPSSRNY SE+DSVEDE+ET
Sbjct: 61  EIIARTLQEEFSQLEMAEASGYSNVEDP-SQASKFALGWNNPSSRNYTSENDSVEDEIET 120

Query: 123 GELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLD 182
            E SSSCSSPG ++N YP G+ +QVDW++DQMI APHVPRINGEIP IDEVTSDHQRLLD
Sbjct: 121 VEPSSSCSSPGQEDNYYPNGHDNQVDWKMDQMIPAPHVPRINGEIPPIDEVTSDHQRLLD 180

Query: 183 RLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGY 242
           RLKVYDFVERKVKGDGNCQFRALSDQLFGTS RH++VR++VVNQLKSHREIYEGYVPM Y
Sbjct: 181 RLKVYDFVERKVKGDGNCQFRALSDQLFGTSDRHKMVRENVVNQLKSHREIYEGYVPMAY 240

Query: 243 DDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFW 302
           DDYLEKM M+GEWGDHVTLQAAADWYGVKIFVMTSFK+ CCIEILP+FQKKKQV+FLSFW
Sbjct: 241 DDYLEKMSMNGEWGDHVTLQAAADWYGVKIFVMTSFKDTCCIEILPNFQKKKQVLFLSFW 300

Query: 303 AEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIFGNKH 336
           AEVHYNSIYPQG VSSVADEQSNDSRKRRKWWIFGNKH
Sbjct: 301 AEVHYNSIYPQGGVSSVADEQSNDSRKRRKWWIFGNKH 337

BLAST of MC01g0539 vs. NCBI nr
Match: XP_031742503.1 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 isoform X2 [Cucumis sativus])

HSP 1 Score: 584 bits (1505), Expect = 1.02e-208
Identity = 295/345 (85.51%), Postives = 310/345 (89.86%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSS--------YSTEYFSDMAQHDVNFYEHYYGNHCNNEV 60
           MVMNDYDSD IRWGL FFNGDS+        YSTEY  D AQHDVNFYEHYYGN+ NNEV
Sbjct: 1   MVMNDYDSDAIRWGLRFFNGDSNGDSNGDMPYSTEYLPDTAQHDVNFYEHYYGNNYNNEV 60

Query: 61  NNVENDEIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSV 120
           NNVENDEIIAHTLQEEFS+LEIAEASGYSNVE+  S+AS FAL WNNPSSRNY SE+DSV
Sbjct: 61  NNVENDEIIAHTLQEEFSQLEIAEASGYSNVEDP-SQASKFALGWNNPSSRNYSSENDSV 120

Query: 121 EDEVETGELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSD 180
           EDEVET E SSSCSSPG  +N YPYG+ +QVDWR+DQMI APHVPRINGEIPSIDEVTSD
Sbjct: 121 EDEVETVEPSSSCSSPGQGDNYYPYGHDNQVDWRMDQMIPAPHVPRINGEIPSIDEVTSD 180

Query: 181 HQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEG 240
           HQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTS RH+LVR++VV+QLKSHREIYEG
Sbjct: 181 HQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSDRHKLVRENVVSQLKSHREIYEG 240

Query: 241 YVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQV 300
           YVPM YDDYLEKM MSGEWGDHVTLQAAADWYGVKIFVMTSFKE CCIEILP+FQKKKQV
Sbjct: 241 YVPMPYDDYLEKMSMSGEWGDHVTLQAAADWYGVKIFVMTSFKETCCIEILPNFQKKKQV 300

Query: 301 IFLSFWAEVHYNSIYPQGDVSSVADEQS-NDSRKRRKWWIFGNKH 336
           IFLSFWAEVHYNSIYPQG    VADEQS NDSRKRRKWWIFGNKH
Sbjct: 301 IFLSFWAEVHYNSIYPQG----VADEQSSNDSRKRRKWWIFGNKH 340

BLAST of MC01g0539 vs. ExPASy TrEMBL
Match: A0A6J1CRW8 (RNA polymerase sigma factor sigF, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111013756 PE=3 SV=1)

HSP 1 Score: 636 bits (1641), Expect = 2.83e-222
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60
           MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI
Sbjct: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60

Query: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120
           IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE
Sbjct: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120

Query: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180
           LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL
Sbjct: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180

Query: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240
           KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD
Sbjct: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240

Query: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300
           YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE
Sbjct: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300

Query: 301 VHYNSIYPQG 310
           VHYNSIYPQG
Sbjct: 301 VHYNSIYPQG 310

BLAST of MC01g0539 vs. ExPASy TrEMBL
Match: A0A5D3C7T8 (RNA polymerase sigma factor sigF OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00180 PE=4 SV=1)

HSP 1 Score: 565 bits (1457), Expect = 1.56e-200
Identity = 296/390 (75.90%), Postives = 310/390 (79.49%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSS----YSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVE 60
           MVMNDYDSD IRWGL FFNGD +    YSTEY    AQ DVNFYEHYYGNHCNNEVNNVE
Sbjct: 27  MVMNDYDSDAIRWGLRFFNGDLNGDMPYSTEYLPVTAQQDVNFYEHYYGNHCNNEVNNVE 86

Query: 61  NDEIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEV 120
           NDEIIAHTLQEEFS+LEIAEASGYSNVE+  S AS FAL WNNPSSR Y SE+DSVEDEV
Sbjct: 87  NDEIIAHTLQEEFSQLEIAEASGYSNVEDP-SHASKFALGWNNPSSRKYSSENDSVEDEV 146

Query: 121 ETGELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAP----------------HVPRIN 180
           ET E SSSCSSPG ++N YPYG+ +QVDWR+DQMI AP                HVPRIN
Sbjct: 147 ETVEPSSSCSSPGQEDNYYPYGHDNQVDWRMDQMIPAPVRFSIIASFYVGTAVEHVPRIN 206

Query: 181 GEIPSIDEVTSDHQRLLD---------------------------------RLKVYDFVE 240
           GEIPSIDEVTSDHQRLLD                                 RLKVYDFVE
Sbjct: 207 GEIPSIDEVTSDHQRLLDSLLFGVFSLRARLLFDIEFWVPIITTVHCLFNLRLKVYDFVE 266

Query: 241 RKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDDYLEKMIM 300
           RKVKGDGNCQFRALSDQLFGTS RH+LVR++VVNQLKSHREIYEGYVPM YDDYLEKM M
Sbjct: 267 RKVKGDGNCQFRALSDQLFGTSDRHKLVRENVVNQLKSHREIYEGYVPMPYDDYLEKMSM 326

Query: 301 SGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAEVHYNSIY 336
           SGEWGDHVTLQAAADWYGVKIFVMTSFKE CCIEILP+FQKKKQVIFLSFWAEVHYNSIY
Sbjct: 327 SGEWGDHVTLQAAADWYGVKIFVMTSFKETCCIEILPNFQKKKQVIFLSFWAEVHYNSIY 386

BLAST of MC01g0539 vs. ExPASy TrEMBL
Match: A0A6J1I6F5 (uncharacterized protein LOC111471149 OS=Cucurbita maxima OX=3661 GN=LOC111471149 PE=4 SV=1)

HSP 1 Score: 550 bits (1417), Expect = 6.94e-195
Identity = 274/336 (81.55%), Postives = 300/336 (89.29%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60
           +VMNDY+SD  RWGL FFNGDSSYSTEYF DMAQHDVNFY++Y+GN CNNEVN+VENDEI
Sbjct: 58  IVMNDYNSDASRWGLRFFNGDSSYSTEYFHDMAQHDVNFYDYYHGNRCNNEVNHVENDEI 117

Query: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120
           IAHTLQEEF++LEIAEASG SN EE   + SNFAL WNN SSRNY SE+DSVEDEVET E
Sbjct: 118 IAHTLQEEFTQLEIAEASGCSNAEEP-CQTSNFALGWNNLSSRNYSSENDSVEDEVETME 177

Query: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180
            SSSCSSPGH++N  PYG+ +QVDW++DQMI APHVPR NGEIP IDEVTSDHQRLLDRL
Sbjct: 178 PSSSCSSPGHEDNYCPYGHENQVDWKMDQMIPAPHVPRTNGEIPPIDEVTSDHQRLLDRL 237

Query: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240
           KV+DFVERKVKGDGN QFRALSDQLFGTS+RH+LVR++VVNQLKSHREIYEGYVPMGYDD
Sbjct: 238 KVFDFVERKVKGDGNSQFRALSDQLFGTSERHKLVRENVVNQLKSHREIYEGYVPMGYDD 297

Query: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300
           YLEKM +SGEWGDHVTLQ AADWYGVKIFVM SFKE C IEI+P+FQKKKQVIFLSFWAE
Sbjct: 298 YLEKMSVSGEWGDHVTLQEAADWYGVKIFVMASFKETC-IEIIPNFQKKKQVIFLSFWAE 357

Query: 301 VHYNSIYPQGDVSSVADEQSNDSRKRRKWWIFGNKH 336
           VHYNSIYPQ D    A  QSND RKRR+WWIFG+KH
Sbjct: 358 VHYNSIYPQRD----AAVQSNDYRKRRRWWIFGSKH 387

BLAST of MC01g0539 vs. ExPASy TrEMBL
Match: A0A6J1IP18 (RNA polymerase sigma factor sigF, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111478657 PE=3 SV=1)

HSP 1 Score: 548 bits (1411), Expect = 1.33e-187
Identity = 265/309 (85.76%), Postives = 288/309 (93.20%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60
           MVMNDYDSD I WGL  FNGDSSYS+EYF DMAQHDVNFYEHYYGNHCNNEVN+VENDEI
Sbjct: 1   MVMNDYDSDAISWGLRLFNGDSSYSSEYFPDMAQHDVNFYEHYYGNHCNNEVNHVENDEI 60

Query: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120
           IAHTLQEEFS+LEIAEASGYSN+E+  S+AS +AL W+NPSSRNYGSE+DS+ED VET E
Sbjct: 61  IAHTLQEEFSQLEIAEASGYSNIEDP-SQASKYALGWDNPSSRNYGSENDSIEDVVETVE 120

Query: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180
            SSSCSSPG +++ YP+G+  QVDW++DQ+I APHVPRINGEIPSIDEVTSDHQRLLDRL
Sbjct: 121 ASSSCSSPGQEDSYYPHGHDSQVDWKMDQVIPAPHVPRINGEIPSIDEVTSDHQRLLDRL 180

Query: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240
           KVYDFVERKVKGDGNCQFRALSDQLFGTS RH+LVR++VVNQLKSHREIYEGYVPM YDD
Sbjct: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSNRHKLVRENVVNQLKSHREIYEGYVPMAYDD 240

Query: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300
           YLEKM M+GEWGDHVTLQAAADWYGVKIFVMTSFKE CCIEILPSFQKK+QVIFLSFWAE
Sbjct: 241 YLEKMSMTGEWGDHVTLQAAADWYGVKIFVMTSFKETCCIEILPSFQKKRQVIFLSFWAE 300

Query: 301 VHYNSIYPQ 309
           VHYNSIYPQ
Sbjct: 301 VHYNSIYPQ 308

BLAST of MC01g0539 vs. ExPASy TrEMBL
Match: A0A6J1EDW3 (RNA polymerase sigma factor sigF, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111433307 PE=3 SV=1)

HSP 1 Score: 546 bits (1406), Expect = 7.56e-187
Identity = 264/309 (85.44%), Postives = 287/309 (92.88%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNNVENDEI 60
           MVMNDYDSD I WGL  FNGDSSYS+EYF DMAQHDVNFYEHYYGNHCNNEVN+VENDEI
Sbjct: 1   MVMNDYDSDAISWGLRLFNGDSSYSSEYFPDMAQHDVNFYEHYYGNHCNNEVNHVENDEI 60

Query: 61  IAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNPSSRNYGSESDSVEDEVETGE 120
           IAHTLQEEFS+LEIAEASGYSN+E+  S+AS + L W+NPSSRNYGSE+DS+ED VET E
Sbjct: 61  IAHTLQEEFSQLEIAEASGYSNIEDP-SQASKYELGWDNPSSRNYGSENDSIEDVVETVE 120

Query: 121 LSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVPRINGEIPSIDEVTSDHQRLLDRL 180
            SSSCSSPG +++ YP+G+  QVDW++DQ+I APHVPRINGEIPSIDEVTSDHQRLLDRL
Sbjct: 121 ASSSCSSPGQEDSYYPHGHDSQVDWKMDQVIPAPHVPRINGEIPSIDEVTSDHQRLLDRL 180

Query: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQSVVNQLKSHREIYEGYVPMGYDD 240
           KVYDFVERKVKGDGNCQFRALSDQLFGTS RH+LVR++VVNQLKSHREIYEGYVPM YDD
Sbjct: 181 KVYDFVERKVKGDGNCQFRALSDQLFGTSNRHKLVRENVVNQLKSHREIYEGYVPMAYDD 240

Query: 241 YLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKENCCIEILPSFQKKKQVIFLSFWAE 300
           YLEKM M+GEWGDHVTLQAAADWYGVKIFVMTSFKE CCIEILPSFQKK+QVIFLSFWAE
Sbjct: 241 YLEKMSMTGEWGDHVTLQAAADWYGVKIFVMTSFKETCCIEILPSFQKKRQVIFLSFWAE 300

Query: 301 VHYNSIYPQ 309
           VHYNSIYPQ
Sbjct: 301 VHYNSIYPQ 308

BLAST of MC01g0539 vs. TAIR 10
Match: AT5G03330.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 166/363 (45.73%), Postives = 229/363 (63.09%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGD-SSYSTEYFSDMAQHDVNFYEH------YYGNHCNNEVN 60
           MV ++ ++ ++ W LG        Y  E   +  +  V  + H      YY  + ++  +
Sbjct: 1   MVSHEENTSIVEWFLGPHPYTYPPYGIEMIHEDDEVAVAHHHHHHQSGEYYREYEDHRSS 60

Query: 61  NVENDEIIAHTLQEEFSRLEIAEASGYS--NVEEEHSRA--------SNFALDWNNPS-S 120
           +V+NDEIIA TLQ++F +LEIAE++ YS  N +++H +         +N    WN+ S +
Sbjct: 61  DVDNDEIIARTLQDDFLQLEIAESNDYSHQNQQQQHQQEGYTNNYSNNNNGYAWNDQSPA 120

Query: 121 RNYGSE---SDSVEDEVETGELS-SSCSSPGHDENSYPYG-NGDQVD------WRLDQMI 180
            +Y SE   +D+ +D      ++  SCSSP  D + Y Y    DQ D       RL+QM+
Sbjct: 121 VDYSSEWIGNDNDQDGRSDDSVNVFSCSSPS-DTDEYVYSWESDQCDADGEFGRRLNQMV 180

Query: 181 TAPHVPRINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKR 240
             P++P+INGEIP  +E  SDH+RL +RL+++DF E KV GDGNCQFRAL+DQL+ T+ R
Sbjct: 181 PIPYIPKINGEIPPEEEAVSDHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKTADR 240

Query: 241 HRLVRQSVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVM 300
           H+ VR+ +V QLKS  + Y+GYVPM + DYL KM  SGEWGDHVTLQAAAD Y VKI V+
Sbjct: 241 HKHVRRQIVKQLKSRPDSYQGYVPMDFSDYLRKMSRSGEWGDHVTLQAAADAYRVKIVVL 300

Query: 301 TSFKENCCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWI 335
           TSFK+ C IEILP+ Q+ K VIFLSFWAEVHYN+IY   D S        + +++RKWW 
Sbjct: 301 TSFKDTCYIEILPTSQESKGVIFLSFWAEVHYNAIYLNRDTS------ETELQRKRKWWR 356

BLAST of MC01g0539 vs. TAIR 10
Match: AT5G03330.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-75
Identity = 166/363 (45.73%), Postives = 229/363 (63.09%), Query Frame = 0

Query: 1   MVMNDYDSDVIRWGLGFFNGD-SSYSTEYFSDMAQHDVNFYEH------YYGNHCNNEVN 60
           MV ++ ++ ++ W LG        Y  E   +  +  V  + H      YY  + ++  +
Sbjct: 1   MVSHEENTSIVEWFLGPHPYTYPPYGIEMIHEDDEVAVAHHHHHHQSGEYYREYEDHRSS 60

Query: 61  NVENDEIIAHTLQEEFSRLEIAEASGYS--NVEEEHSRA--------SNFALDWNNPS-S 120
           +V+NDEIIA TLQ++F +LEIAE++ YS  N +++H +         +N    WN+ S +
Sbjct: 61  DVDNDEIIARTLQDDFLQLEIAESNDYSHQNQQQQHQQEGYTNNYSNNNNGYAWNDQSPA 120

Query: 121 RNYGSE---SDSVEDEVETGELS-SSCSSPGHDENSYPYG-NGDQVD------WRLDQMI 180
            +Y SE   +D+ +D      ++  SCSSP  D + Y Y    DQ D       RL+QM+
Sbjct: 121 VDYSSEWIGNDNDQDGRSDDSVNVFSCSSPS-DTDEYVYSWESDQCDADGEFGRRLNQMV 180

Query: 181 TAPHVPRINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKR 240
             P++P+INGEIP  +E  SDH+RL +RL+++DF E KV GDGNCQFRAL+DQL+ T+ R
Sbjct: 181 PIPYIPKINGEIPPEEEAVSDHERLRNRLEMFDFTEVKVPGDGNCQFRALADQLYKTADR 240

Query: 241 HRLVRQSVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVM 300
           H+ VR+ +V QLKS  + Y+GYVPM + DYL KM  SGEWGDHVTLQAAAD Y VKI V+
Sbjct: 241 HKHVRRQIVKQLKSRPDSYQGYVPMDFSDYLRKMSRSGEWGDHVTLQAAADAYRVKIVVL 300

Query: 301 TSFKENCCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWI 335
           TSFK+ C IEILP+ Q+ K VIFLSFWAEVHYN+IY   D S        + +++RKWW 
Sbjct: 301 TSFKDTCYIEILPTSQESKGVIFLSFWAEVHYNAIYLNRDTS------ETELQRKRKWWR 356

BLAST of MC01g0539 vs. TAIR 10
Match: AT5G04250.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 9.7e-72
Identity = 163/355 (45.92%), Postives = 212/355 (59.72%), Query Frame = 0

Query: 7   DSDVIRWGLGFFN-------GDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNN-VEND 66
           D D +RWGL           G  S  T Y S          E Y     N  V   V+ND
Sbjct: 6   DPDALRWGLHDLEVCTLTNAGSCSSVTRYESGGGGTQGYVREGY-----NQPVTGYVDND 65

Query: 67  EIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNP--SSRNYG------SESD 126
            +IA   Q+E SR+  AEASG +++    S  S  A DW +P     N G       ESD
Sbjct: 66  AVIAQFYQDELSRVARAEASGINSL----SPTSVVAQDWPHPHQGQENQGEAIDITQESD 125

Query: 127 -------SVEDE-VETGELSSSCSSPGHDENSYPYGNGDQVDW-----RLDQMITAPHVP 186
                  ++ED+ V         SSP  D++S      ++  W     RL+QMI   HVP
Sbjct: 126 ILHNHNGNMEDKNVARIRFEGGQSSPSRDDDSVCSVEIEEESWSEVGKRLNQMIPIAHVP 185

Query: 187 RINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQ 246
           +INGE+PS DE  SDH+RL  RL++Y  VE K++GDGNCQFR+LSDQL+ + + H  VR+
Sbjct: 186 KINGELPSEDEQISDHERLFQRLQLYGLVENKIEGDGNCQFRSLSDQLYRSPEHHNFVRE 245

Query: 247 SVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKEN 306
            VVNQL  +REIYEGYVPM Y+DYL+ M  +GEWGDHVTLQAAAD +GV++FV+TSFK+ 
Sbjct: 246 QVVNQLAYNREIYEGYVPMAYNDYLKAMKRNGEWGDHVTLQAAADLFGVRMFVITSFKDT 305

Query: 307 CCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIF 333
           C IEILP FQK  ++I LSFWAEVHYNSIYP+G++         + +K++K+W+F
Sbjct: 306 CYIEILPHFQKSNRLICLSFWAEVHYNSIYPEGEL------PIPEGKKKKKYWVF 345

BLAST of MC01g0539 vs. TAIR 10
Match: AT5G04250.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 9.7e-72
Identity = 163/355 (45.92%), Postives = 212/355 (59.72%), Query Frame = 0

Query: 7   DSDVIRWGLGFFN-------GDSSYSTEYFSDMAQHDVNFYEHYYGNHCNNEVNN-VEND 66
           D D +RWGL           G  S  T Y S          E Y     N  V   V+ND
Sbjct: 6   DPDALRWGLHDLEVCTLTNAGSCSSVTRYESGGGGTQGYVREGY-----NQPVTGYVDND 65

Query: 67  EIIAHTLQEEFSRLEIAEASGYSNVEEEHSRASNFALDWNNP--SSRNYG------SESD 126
            +IA   Q+E SR+  AEASG +++    S  S  A DW +P     N G       ESD
Sbjct: 66  AVIAQFYQDELSRVARAEASGINSL----SPTSVVAQDWPHPHQGQENQGEAIDITQESD 125

Query: 127 -------SVEDE-VETGELSSSCSSPGHDENSYPYGNGDQVDW-----RLDQMITAPHVP 186
                  ++ED+ V         SSP  D++S      ++  W     RL+QMI   HVP
Sbjct: 126 ILHNHNGNMEDKNVARIRFEGGQSSPSRDDDSVCSVEIEEESWSEVGKRLNQMIPIAHVP 185

Query: 187 RINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQ 246
           +INGE+PS DE  SDH+RL  RL++Y  VE K++GDGNCQFR+LSDQL+ + + H  VR+
Sbjct: 186 KINGELPSEDEQISDHERLFQRLQLYGLVENKIEGDGNCQFRSLSDQLYRSPEHHNFVRE 245

Query: 247 SVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKEN 306
            VVNQL  +REIYEGYVPM Y+DYL+ M  +GEWGDHVTLQAAAD +GV++FV+TSFK+ 
Sbjct: 246 QVVNQLAYNREIYEGYVPMAYNDYLKAMKRNGEWGDHVTLQAAADLFGVRMFVITSFKDT 305

Query: 307 CCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIF 333
           C IEILP FQK  ++I LSFWAEVHYNSIYP+G++         + +K++K+W+F
Sbjct: 306 CYIEILPHFQKSNRLICLSFWAEVHYNSIYPEGEL------PIPEGKKKKKYWVF 345

BLAST of MC01g0539 vs. TAIR 10
Match: AT3G02070.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 109/235 (46.38%), Postives = 147/235 (62.55%), Query Frame = 0

Query: 98  NNPSSRNYGSESDSVEDEVETGELSSSCSSPGHDENSYPYGNGDQVDWRLDQMITAPHVP 157
           ++ SS ++ S+ D+ +D +    LS   S                V  RL  +   PHVP
Sbjct: 3   DSSSSTSWSSKKDTEDDRMIAFMLSEEYSK-----------LDGAVGRRLSNLAPVPHVP 62

Query: 158 RINGEIPSIDEVTSDHQRLLDRLKVYDFVERKVKGDGNCQFRALSDQLFGTSKRHRLVRQ 217
           RIN  IP++++ T DHQRLL RL VY   E KV GDGNCQFRALSDQL+ + + H+ VR+
Sbjct: 63  RINCYIPNLNDATLDHQRLLQRLNVYGLCELKVSGDGNCQFRALSDQLYRSPEYHKQVRR 122

Query: 218 SVVNQLKSHREIYEGYVPMGYDDYLEKMIMSGEWGDHVTLQAAADWYGVKIFVMTSFKEN 277
            VV QLK  R +YE YVPM Y  Y +KM   GEWGDH+TLQAAAD +  KI ++TSF++ 
Sbjct: 123 EVVKQLKECRSMYESYVPMKYKRYYKKMGKFGEWGDHITLQAAADRFAAKICLLTSFRDT 182

Query: 278 CCIEILPSFQKKKQVIFLSFWAEVHYNSIYPQGDVSSVADEQSNDSRKRRKWWIF 333
           C IEI+P +Q  K V++LSFW+EVHYNS+Y   D+ +   +     + +RK W+F
Sbjct: 183 CFIEIIPQYQAPKGVLWLSFWSEVHYNSLY---DIQAAPVQH----KPKRKHWLF 219

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZF74.5e-7445.73OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10 OS=Arabidopsis thalia... [more]
Q8LBW21.4e-7045.92OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 9 OS=Arabidopsis thalian... [more]
Q9SGA52.9e-5246.38OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 OS=Arabidopsis thalia... [more]
Q0V8694.0e-4643.43OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 11 OS=Arabidopsis thalia... [more]
Q54P703.1e-2238.85OTU domain-containing protein DDB_G0284757 OS=Dictyostelium discoideum OX=44689 ... [more]
Match NameE-valueIdentityDescription
XP_022143971.15.85e-222100.00RNA polymerase sigma factor sigF, chloroplastic [Momordica charantia][more]
KAG6604409.19.29e-21385.12OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10, partial [Cucurbita a... [more]
XP_031742500.11.30e-21286.38OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 isoform X1 [Cucumis s... [more]
XP_038882896.11.17e-21185.80OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 10-like isoform X1 [Beni... [more]
XP_031742503.11.02e-20885.51OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 12 isoform X2 [Cucumis s... [more]
Match NameE-valueIdentityDescription
A0A6J1CRW82.83e-222100.00RNA polymerase sigma factor sigF, chloroplastic OS=Momordica charantia OX=3673 G... [more]
A0A5D3C7T81.56e-20075.90RNA polymerase sigma factor sigF OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1I6F56.94e-19581.55uncharacterized protein LOC111471149 OS=Cucurbita maxima OX=3661 GN=LOC111471149... [more]
A0A6J1IP181.33e-18785.76RNA polymerase sigma factor sigF, chloroplastic OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1EDW37.56e-18785.44RNA polymerase sigma factor sigF, chloroplastic-like OS=Cucurbita moschata OX=36... [more]
Match NameE-valueIdentityDescription
AT5G03330.13.2e-7545.73Cysteine proteinases superfamily protein [more]
AT5G03330.23.2e-7545.73Cysteine proteinases superfamily protein [more]
AT5G04250.19.7e-7245.92Cysteine proteinases superfamily protein [more]
AT5G04250.29.7e-7245.92Cysteine proteinases superfamily protein [more]
AT3G02070.12.0e-5346.38Cysteine proteinases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003323OTU domainPFAMPF02338OTUcoord: 191..278
e-value: 2.5E-10
score: 40.9
IPR003323OTU domainPROSITEPS50802OTUcoord: 185..309
score: 17.214411
NoneNo IPR availableGENE3D3.90.70.80coord: 166..315
e-value: 3.4E-40
score: 139.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 100..137
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..133
NoneNo IPR availablePANTHERPTHR12419OTU DOMAIN CONTAINING PROTEINcoord: 24..322
NoneNo IPR availablePANTHERPTHR12419:SF78OTU DOMAIN-CONTAINING PROTEIN DDB_G0284757-LIKE ISOFORM X1coord: 24..322
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 172..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0539.1MC01g0539.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071482 cellular response to light stimulus
biological_process GO:2000142 regulation of DNA-templated transcription, initiation
molecular_function GO:0003677 DNA binding
molecular_function GO:0016987 sigma factor activity