MC01g0504 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0504
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionMediator of RNA polymerase II transcription subunit 4
LocationMC01: 11489697 .. 11492428 (+)
RNA-Seq ExpressionMC01g0504
SyntenyMC01g0504
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACGGTCCTAAATATTTTATTTTGGTTATTTTTTTAAAATAGGGGATAAATAAGGACAACTGCTCTCAAATTTTTGTATACAAAGCTACCGAATATACCAAGGCCCATTCTATTTCTCCCAGCCCAATCTAATCCCACCATTTCCAGCCCATCAGCCCAGCTCCCAGCCCACTTTATTCAAGAAAAGGAAAAAAAAAAGGAAAATCGATCATTAATTTGCAAAACGAAATCGCCTGCTCTCCCTCCCTATTCTGTACTGTCGTCGCCGCTGCTCGGACTCTGTACTGCCATGGCGCCCACCCATTGGCTTCTCCGGCGACCTCCTCTCACGCGCCGCTCTTCCCTCTAGCTCGTGCCCTCTCGCGCCGATCTTCTTCTTCTTCTTCTTCTCCTCGATCTCAGGTTCTCTCAATTCCTGTACTTGTACTTGTCTTTCAGGTTTCAGATTCTTATTTTTCTGTGTTCTGTTTGAATTTAACTCGTAATACGATCCATTGAAGAGCAAATCAATATGCTTCAGCACCAAATTGCGCAGTCCCCTGCTAGGTTGGGCTTGAACAATCCCAATTCTCCCTCAATTCAAAACCCTACCCCTCCCAAATTGCCTCCTTCTCAACAATCCCACCACCAAAATCAACCCAATCTCTCTTCTCCAACTCCTTCATCGACCCTTCTCCCTCTCCTTCCTCCGCTCCCCAGAGCTCAAGCTCTTCTCCTGCAAATGGCTACCCTCGCCTCCAAGCTCTTTGAAGTCTCCCCCAATCGCTCCCTTTGGCTCACTGCGTTCCGGGGTTCTCTCCCAACTTTTCTTAATTCCCAAACGCAGTCGTCGCCCTCTACGCCTCTTGATGCTACTCCATCTACTTCGAAAGAAATTCTGTCCCTGTTTAATGTTCTGCAGACCCAGTTGTTTGAAGCTGTTGCCGAGCTTCAGGAGATTCTTGATCTTCAGGACTCTAAGCAGAAGATTGCCTATGAGATTCGGGCCAAGGATGCTGCTCTTCGTGCTTTTGCTAATAAACTCAAGGATGCTGAGCAGGTTCTTGATGTTCTGTTTGATGATTATGCGGATTATCGACGTCCTCAACGGGTTAAGACAAAGGATGATGTTGAAAATGATTCTTTGTGTTCGACTACGGTTGCATCCCAGTTGAAATTATCTGATATATTGTCGTATGCCCATCGAATTAGCTATACGACTTTCGCGCCACCTGAATTCGGCGCTGGACAAGCTCCGCTACGTGGAGCTCTTCCTCCCGCCCCGCAAGAAGAGCAAATGCGAGCCTCCCAGTTGTATAACTTTGCAGACCTTGATGTTGGCTTGCCTAAAGCAGTTGAAAGCACTGAGAAAACGATTGAGCCAATGATTGAGCCTCCAGCTCCAGTGCCAACGGATGCTAATCCTCTTGCTAATCTGGCTGCTATTCAGGGTCTGCTTCCTCCAAATATCACTGTACCATCTGGTTGGAAACCTGGAATGCCAGTGGAATTGCCCAGAGATTTACCAATGCCCCCTCCTGGATGGAAGCCTGGGGACCCTGTGCCATTACCCCCATTGGATTCTCTTCCTGTTCCTAGAGTTGATGAGCCACAACAATTGAGGCCTAATGCTCTTCCAGGATTGCACAAACCTCCTGAGCCGATACAAGTTAGGCCTGTACAGTTGGATATTCTTGATCAAGATGATGATAGTAGTGATTACACTAGTGATGAAGGAAGCTCTGAAGATGATGATTAATGGAAACGGCTTGAATCACCAACTAAGACCCTTTAATGACCGCTTGATGATAAGAGGTGCAGAGACAGTCGAGAAGTTTGCTTAGGAGCTGCCACCCTTGAAAGAGTGTGTGCAATGATGCACAGGACTAGGAGATCTGTTAAAGGTTACATTACATTACGCACTTTCAAGCTTACAAGTTACTGATGATGTGTTATGCATTTACAAGGTCAACTAAAAGGAAAACACTTCAAATCTGACTTAGTTCTCTTACACTTTTATTTTGTTTTTAACGCGAGCTGAAACAAACTCTCTTAGGATACCATTTAAACATGACTAAAGAGTGTTCCGACTGTAATACCGTGTTGTAGTGCGATTGGTAAATAAGCTTCTATAGGCTGCTGAATCAATTATTGACTCAATATTGTGATTTGAAGGAAATTTATCGATTATTTGATGTTGCTGGTATTTAGAGCAAACTGTTGTGTATTACAAGTTTGATGAGATTCTGTGGAATGTAACTTGGTACTTGTGAAAGAGAACGTATTAATATGAAGAATACTGTTGTCCAAACATTTTTTCTCTTCTTCAGAATAACAGTAGTGTGGTGAGCAGAGGATGAAGATTTAGGAACTATTTGAAAAGCTATGCTGCCTAGTATTTTCCATGACATGCCTGGCTATATTAGAAGAGAAGAGGCTTCAGATTCTGATGTTTCGATGCAGCTGGATGATGGGAATTTATGAATTTTATGGTGTCCATAGAATTTTCTTATTATTGAAGATGGTTGAAGAACATCTTATGATCATAGACTAAAAGTATATGTATGTGAAGGCATTAATATTGAATTGCCTAATGAATACAGATCAAGGTTAAAACTACTTTTTTTTTTTTTTGTGGTAATAAAACACAATACTTCAAAATGACAAATTTTTTAGGAAAAGAAAAATCATAATGATGTTTGTTTTTGGGAAAACACATGATCATGAAAGTTGATATTATGGTTGGTTCATT

mRNA sequence

CAACGGTCCTAAATATTTTATTTTGGTTATTTTTTTAAAATAGGGGATAAATAAGGACAACTGCTCTCAAATTTTTGTATACAAAGCTACCGAATATACCAAGGCCCATTCTATTTCTCCCAGCCCAATCTAATCCCACCATTTCCAGCCCATCAGCCCAGCTCCCAGCCCACTTTATTCAAGAAAAGGAAAAAAAAAAGGAAAATCGATCATTAATTTGCAAAACGAAATCGCCTGCTCTCCCTCCCTATTCTGTACTGTCGTCGCCGCTGCTCGGACTCTGTACTGCCATGGCGCCCACCCATTGGCTTCTCCGGCGACCTCCTCTCACGCGCCGCTCTTCCCTCTAGCTCGTGCCCTCTCGCGCCGATCTTCTTCTTCTTCTTCTTCTCCTCGATCTCAGAGCAAATCAATATGCTTCAGCACCAAATTGCGCAGTCCCCTGCTAGGTTGGGCTTGAACAATCCCAATTCTCCCTCAATTCAAAACCCTACCCCTCCCAAATTGCCTCCTTCTCAACAATCCCACCACCAAAATCAACCCAATCTCTCTTCTCCAACTCCTTCATCGACCCTTCTCCCTCTCCTTCCTCCGCTCCCCAGAGCTCAAGCTCTTCTCCTGCAAATGGCTACCCTCGCCTCCAAGCTCTTTGAAGTCTCCCCCAATCGCTCCCTTTGGCTCACTGCGTTCCGGGGTTCTCTCCCAACTTTTCTTAATTCCCAAACGCAGTCGTCGCCCTCTACGCCTCTTGATGCTACTCCATCTACTTCGAAAGAAATTCTGTCCCTGTTTAATGTTCTGCAGACCCAGTTGTTTGAAGCTGTTGCCGAGCTTCAGGAGATTCTTGATCTTCAGGACTCTAAGCAGAAGATTGCCTATGAGATTCGGGCCAAGGATGCTGCTCTTCGTGCTTTTGCTAATAAACTCAAGGATGCTGAGCAGGTTCTTGATGTTCTGTTTGATGATTATGCGGATTATCGACGTCCTCAACGGGTTAAGACAAAGGATGATGTTGAAAATGATTCTTTGTGTTCGACTACGGTTGCATCCCAGTTGAAATTATCTGATATATTGTCGTATGCCCATCGAATTAGCTATACGACTTTCGCGCCACCTGAATTCGGCGCTGGACAAGCTCCGCTACGTGGAGCTCTTCCTCCCGCCCCGCAAGAAGAGCAAATGCGAGCCTCCCAGTTGTATAACTTTGCAGACCTTGATGTTGGCTTGCCTAAAGCAGTTGAAAGCACTGAGAAAACGATTGAGCCAATGATTGAGCCTCCAGCTCCAGTGCCAACGGATGCTAATCCTCTTGCTAATCTGGCTGCTATTCAGGGTCTGCTTCCTCCAAATATCACTGTACCATCTGGTTGGAAACCTGGAATGCCAGTGGAATTGCCCAGAGATTTACCAATGCCCCCTCCTGGATGGAAGCCTGGGGACCCTGTGCCATTACCCCCATTGGATTCTCTTCCTGTTCCTAGAGTTGATGAGCCACAACAATTGAGGCCTAATGCTCTTCCAGGATTGCACAAACCTCCTGAGCCGATACAAGTTAGGCCTGTACAGTTGGATATTCTTGATCAAGATGATGATAGTAGTGATTACACTAGTGATGAAGGAAGCTCTGAAGATGATGATTAATGGAAACGGCTTGAATCACCAACTAAGACCCTTTAATGACCGCTTGATGATAAGAGGTGCAGAGACAGTCGAGAAGTTTGCTTAGGAGCTGCCACCCTTGAAAGAGTGTGTGCAATGATGCACAGGACTAGGAGATCTGTTAAAGGTTACATTACATTACGCACTTTCAAGCTTACAAGTTACTGATGATGTGTTATGCATTTACAAGGTCAACTAAAAGGAAAACACTTCAAATCTGACTTAGTTCTCTTACACTTTTATTTTGTTTTTAACGCGAGCTGAAACAAACTCTCTTAGGATACCATTTAAACATGACTAAAGAGTGTTCCGACTGTAATACCGTGTTGTAGTGCGATTGGTAAATAAGCTTCTATAGGCTGCTGAATCAATTATTGACTCAATATTGTGATTTGAAGGAAATTTATCGATTATTTGATGTTGCTGGTATTTAGAGCAAACTGTTGTGTATTACAAGTTTGATGAGATTCTGTGGAATGTAACTTGGTACTTGTGAAAGAGAACGTATTAATATGAAGAATACTGTTGTCCAAACATTTTTTCTCTTCTTCAGAATAACAGTAGTGTGGTGAGCAGAGGATGAAGATTTAGGAACTATTTGAAAAGCTATGCTGCCTAGTATTTTCCATGACATGCCTGGCTATATTAGAAGAGAAGAGGCTTCAGATTCTGATGTTTCGATGCAGCTGGATGATGGGAATTTATGAATTTTATGGTGTCCATAGAATTTTCTTATTATTGAAGATGGTTGAAGAACATCTTATGATCATAGACTAAAAGTATATGTATGTGAAGGCATTAATATTGAATTGCCTAATGAATACAGATCAAGGTTAAAACTACTTTTTTTTTTTTTTGTGGTAATAAAACACAATACTTCAAAATGACAAATTTTTTAGGAAAAGAAAAATCATAATGATGTTTGTTTTTGGGAAAACACATGATCATGAAAGTTGATATTATGGTTGGTTCATT

Coding sequence (CDS)

ATGCTTCAGCACCAAATTGCGCAGTCCCCTGCTAGGTTGGGCTTGAACAATCCCAATTCTCCCTCAATTCAAAACCCTACCCCTCCCAAATTGCCTCCTTCTCAACAATCCCACCACCAAAATCAACCCAATCTCTCTTCTCCAACTCCTTCATCGACCCTTCTCCCTCTCCTTCCTCCGCTCCCCAGAGCTCAAGCTCTTCTCCTGCAAATGGCTACCCTCGCCTCCAAGCTCTTTGAAGTCTCCCCCAATCGCTCCCTTTGGCTCACTGCGTTCCGGGGTTCTCTCCCAACTTTTCTTAATTCCCAAACGCAGTCGTCGCCCTCTACGCCTCTTGATGCTACTCCATCTACTTCGAAAGAAATTCTGTCCCTGTTTAATGTTCTGCAGACCCAGTTGTTTGAAGCTGTTGCCGAGCTTCAGGAGATTCTTGATCTTCAGGACTCTAAGCAGAAGATTGCCTATGAGATTCGGGCCAAGGATGCTGCTCTTCGTGCTTTTGCTAATAAACTCAAGGATGCTGAGCAGGTTCTTGATGTTCTGTTTGATGATTATGCGGATTATCGACGTCCTCAACGGGTTAAGACAAAGGATGATGTTGAAAATGATTCTTTGTGTTCGACTACGGTTGCATCCCAGTTGAAATTATCTGATATATTGTCGTATGCCCATCGAATTAGCTATACGACTTTCGCGCCACCTGAATTCGGCGCTGGACAAGCTCCGCTACGTGGAGCTCTTCCTCCCGCCCCGCAAGAAGAGCAAATGCGAGCCTCCCAGTTGTATAACTTTGCAGACCTTGATGTTGGCTTGCCTAAAGCAGTTGAAAGCACTGAGAAAACGATTGAGCCAATGATTGAGCCTCCAGCTCCAGTGCCAACGGATGCTAATCCTCTTGCTAATCTGGCTGCTATTCAGGGTCTGCTTCCTCCAAATATCACTGTACCATCTGGTTGGAAACCTGGAATGCCAGTGGAATTGCCCAGAGATTTACCAATGCCCCCTCCTGGATGGAAGCCTGGGGACCCTGTGCCATTACCCCCATTGGATTCTCTTCCTGTTCCTAGAGTTGATGAGCCACAACAATTGAGGCCTAATGCTCTTCCAGGATTGCACAAACCTCCTGAGCCGATACAAGTTAGGCCTGTACAGTTGGATATTCTTGATCAAGATGATGATAGTAGTGATTACACTAGTGATGAAGGAAGCTCTGAAGATGATGATTAA

Protein sequence

MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPPLPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDVLFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLANLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEPQQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD
Homology
BLAST of MC01g0504 vs. ExPASy Swiss-Prot
Match: Q9LZ00 (Mediator of RNA polymerase II transcription subunit 4 OS=Arabidopsis thaliana OX=3702 GN=MED4 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.5e-140
Identity = 286/434 (65.90%), Postives = 337/434 (77.65%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTP-----PKLPPSQQSHH---QNQPNL------- 60
           MLQHQI QSPARLGL  P SPS+QNPTP     P    S QS H   Q QPNL       
Sbjct: 1   MLQHQIVQSPARLGLTGPGSPSVQNPTPTRHGHPTSSSSSQSQHQQIQQQPNLLPSSTVA 60

Query: 61  -------SSPTPSSTLLPLLPPLPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPT 120
                  S+   SS LL LLPPLPRAQALL QMA L SKLF+VSPNR++WL+AFRGSLP+
Sbjct: 61  AASSASASAAVSSSALLSLLPPLPRAQALLQQMAVLTSKLFDVSPNRAIWLSAFRGSLPS 120

Query: 121 FLNSQTQSSPSTPLDATPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIR 180
           FL+S +   P    + +PS++KEILS FN LQTQLFEAV ELQEILDLQD+KQK+A EI+
Sbjct: 121 FLSSHSLPPPPPLENPSPSSTKEILSQFNSLQTQLFEAVTELQEILDLQDAKQKVAREIK 180

Query: 181 AKDAALRAFANKLKDAEQVLDVLFDDYADYRRPQRVK-TKDDVEND---SLCSTTVASQL 240
           +KD++L AFANKLKDAE+VLD+L DDY+DYR+P+R K  +DD +ND   S  STTV+SQL
Sbjct: 181 SKDSSLLAFANKLKDAERVLDMLVDDYSDYRKPKRSKIEEDDEDNDNESSSSSTTVSSQL 240

Query: 241 KLSDILSYAHRISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKA 300
           KL DIL+YAH+ISYTTFAPPEFGAGQAPLRGALPPAPQ+EQMRASQLY FADLD+GLPK 
Sbjct: 241 KLKDILAYAHKISYTTFAPPEFGAGQAPLRGALPPAPQDEQMRASQLYTFADLDIGLPKT 300

Query: 301 VESTEKTIEPMIEPPAPVPTDANPLANLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMP 360
           VE+ EK +E +IEPP P         +++AI  LLPPNI VPSGWKPGMPVELPRDLP+P
Sbjct: 301 VENMEKKVEALIEPPPPPEA-----MDISAIHNLLPPNIAVPSGWKPGMPVELPRDLPLP 360

Query: 361 PPGWKPGDPVPLPPLDSLPVPRVDEPQQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDS 409
           PPGWKPGDPV LPPL+S+  PR ++ Q +RP+   GLH+PP+ IQVR VQLDIL +DDDS
Sbjct: 361 PPGWKPGDPVVLPPLESIAAPRAEDHQHMRPS--QGLHRPPDVIQVRAVQLDIL-EDDDS 420

BLAST of MC01g0504 vs. ExPASy Swiss-Prot
Match: Q54D00 (Putative mediator of RNA polymerase II transcription subunit 4 OS=Dictyostelium discoideum OX=44689 GN=med4 PE=3 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.9e-05
Identity = 57/209 (27.27%), Postives = 89/209 (42.58%), Query Frame = 0

Query: 100 LNSQTQSSPSTPLD-----ATPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIA 159
           LNSQ       P+D     ATP+   ++++    +       + +L++   +Q    +I 
Sbjct: 44  LNSQQLQQIQLPIDQISNNATPNMVLQLMNHLIDVDKIYQNTLKKLEKHQKIQKEITEIQ 103

Query: 160 YEIRAKDAALRAFANKLKDAEQVLD------------------VLFDDYADYRRPQRVKT 219
            EI  KD  +   A  LKD E  L+                  ++ +   D ++P    +
Sbjct: 104 KEIEEKDKLISTLALNLKDIESFLENELSQDNVNINNGNASDGIVNEVLIDLKQPDDAAS 163

Query: 220 KDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFG-AGQAPLRGAL-PPAPQEE 273
            D++ N          ++   D++SYAH+IS TT AP  FG    APL     PPAPQ+E
Sbjct: 164 MDNIVN---------REVSPEDLISYAHKISGTTSAP--FGYQPNAPLASLFKPPAPQDE 223

BLAST of MC01g0504 vs. NCBI nr
Match: XP_022143910.1 (mediator of RNA polymerase II transcription subunit 4 [Momordica charantia])

HSP 1 Score: 778 bits (2010), Expect = 2.61e-283
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP
Sbjct: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK
Sbjct: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA
Sbjct: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
           NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP
Sbjct: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408

BLAST of MC01g0504 vs. NCBI nr
Match: XP_038882754.1 (mediator of RNA polymerase II transcription subunit 4 [Benincasa hispida])

HSP 1 Score: 729 bits (1883), Expect = 5.65e-264
Identity = 384/408 (94.12%), Postives = 397/408 (97.30%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQNQ N+SSPTPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLANPNSPSIQNPTPPKLPPSQQSHHQNQLNISSPTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEVSPNRSLWLTAFRGSLPTFL SQTQSSPST LD+TPSTSK
Sbjct: 61  LPRAQALLLQMASLASKLFEVSPNRSLWLTAFRGSLPTFLTSQTQSSPSTSLDSTPSTSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADY+RP+RVK KDD EN+SLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYQRPRRVKMKDDAENESLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQ+EQMRASQLYNFA+LD+GLPKAVESTEKTIEPMIEPPAPVPTDANPL+
Sbjct: 241 APLRGALPPAPQDEQMRASQLYNFANLDIGLPKAVESTEKTIEPMIEPPAPVPTDANPLS 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
           N+A+IQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPP+DSLPV RVDEP
Sbjct: 301 NMASIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPMDSLPVTRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           Q LRPNA PGLHKPPEPIQVR VQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 Q-LRPNAHPGLHKPPEPIQVRHVQLDILDQDDDSSDYTSDEGSSEDDD 407

BLAST of MC01g0504 vs. NCBI nr
Match: XP_008449288.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 4 [Cucumis melo] >KAA0047287.1 mediator of RNA polymerase II transcription subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 712 bits (1837), Expect = 5.76e-257
Identity = 378/408 (92.65%), Postives = 392/408 (96.08%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQNQ N+SS TPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNQSNVSSSTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEVSPNRSLWLTAFRGSLPTFL SQTQSSPST LD+TPS+SK
Sbjct: 61  LPRAQALLLQMASLASKLFEVSPNRSLWLTAFRGSLPTFLTSQTQSSPSTFLDSTPSSSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADY+RP+RVKT+   EN+SLCSTTVASQL+LSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYQRPRRVKTEGGAENESLCSTTVASQLRLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQ+EQMRASQLYNFA+LDVGLPKAVESTEKTIEPMIEPPAPV  DANPL+
Sbjct: 241 APLRGALPPAPQDEQMRASQLYNFANLDVGLPKAVESTEKTIEPMIEPPAPVRVDANPLS 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
           N+AAIQGLLPPNITVPSGWKPGMPVELP DLPMPPPGWKPGDPV LPP+DSLPV RVDEP
Sbjct: 301 NMAAIQGLLPPNITVPSGWKPGMPVELPGDLPMPPPGWKPGDPVRLPPMDSLPVTRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           Q LRPNALPGLHKPPEPIQVR VQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 Q-LRPNALPGLHKPPEPIQVRHVQLDILDQDDDSSDYTSDEGSSEDDD 407

BLAST of MC01g0504 vs. NCBI nr
Match: XP_023518528.1 (mediator of RNA polymerase II transcription subunit 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708 bits (1828), Expect = 1.35e-255
Identity = 380/409 (92.91%), Postives = 393/409 (96.09%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQNQ NLSSPTPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNQSNLSSPTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEV PNR+LWLTAFRGSLPTFLNSQTQS+PSTPLD+TPSTSK
Sbjct: 61  LPRAQALLLQMASLASKLFEVYPNRALWLTAFRGSLPTFLNSQTQSAPSTPLDSTPSTSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYE+RAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEMRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCS-TTVASQLKLSDILSYAHRISYTTFAPPEFGAG 240
           LFDDYADY+  +RVKT DDVEN+SLCS TTVASQLKLSDILSYAH+ISYTTFAPPEFGAG
Sbjct: 181 LFDDYADYQGHKRVKTTDDVENESLCSSTTVASQLKLSDILSYAHKISYTTFAPPEFGAG 240

Query: 241 QAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPL 300
           QAPLRGALPPAPQEEQMRASQLY+FADLDVGLPK+VESTEKTIEPMIEPPAPVPTDANPL
Sbjct: 241 QAPLRGALPPAPQEEQMRASQLYSFADLDVGLPKSVESTEKTIEPMIEPPAPVPTDANPL 300

Query: 301 ANLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDE 360
           A LA IQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPV RVDE
Sbjct: 301 A-LAGIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVARVDE 360

Query: 361 PQQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           PQ LR NALPGLH+P EPIQVR VQLDILDQDDDSSDY S+EGSSEDDD
Sbjct: 361 PQ-LRHNALPGLHRPQEPIQVRHVQLDILDQDDDSSDYASEEGSSEDDD 407

BLAST of MC01g0504 vs. NCBI nr
Match: XP_022963248.1 (mediator of RNA polymerase II transcription subunit 4 [Cucurbita moschata] >KAG7027281.1 Mediator of RNA polymerase II transcription subunit 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 703 bits (1814), Expect = 1.84e-253
Identity = 378/409 (92.42%), Postives = 390/409 (95.35%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQN  NLSSPTPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNPSNLSSPTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEV PNR+LWLTAFRGSLPTFLNSQTQS+PSTPLD+TPSTSK
Sbjct: 61  LPRAQALLLQMASLASKLFEVYPNRALWLTAFRGSLPTFLNSQTQSAPSTPLDSTPSTSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYE+RAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEMRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTT-VASQLKLSDILSYAHRISYTTFAPPEFGAG 240
           LFDDYADY+  +RVKT DDVEN+SLCSTT VASQLKLSDILSYAH+ISYTTFAPPEFGAG
Sbjct: 181 LFDDYADYQGHKRVKTSDDVENESLCSTTTVASQLKLSDILSYAHKISYTTFAPPEFGAG 240

Query: 241 QAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPL 300
           QAPLRGALPPAPQEEQMRASQLY FADLDVGLPK+VESTEKTIEPMIE PAPVPTDANPL
Sbjct: 241 QAPLRGALPPAPQEEQMRASQLYTFADLDVGLPKSVESTEKTIEPMIEAPAPVPTDANPL 300

Query: 301 ANLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDE 360
           A LA IQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPV RVDE
Sbjct: 301 A-LAGIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVARVDE 360

Query: 361 PQQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           PQ LR NALPGLH+P EPIQVR VQLDILDQDDDSSDY S+EGSSEDDD
Sbjct: 361 PQ-LRHNALPGLHRPQEPIQVRHVQLDILDQDDDSSDYASEEGSSEDDD 407

BLAST of MC01g0504 vs. ExPASy TrEMBL
Match: A0A6J1CQ51 (Mediator of RNA polymerase II transcription subunit 4 OS=Momordica charantia OX=3673 GN=LOC111013711 PE=3 SV=1)

HSP 1 Score: 778 bits (2010), Expect = 1.26e-283
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP
Sbjct: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK
Sbjct: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA
Sbjct: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
           NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP
Sbjct: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408

BLAST of MC01g0504 vs. ExPASy TrEMBL
Match: A0A5A7TZC1 (Mediator of RNA polymerase II transcription subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=MED4 PE=3 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 2.79e-257
Identity = 378/408 (92.65%), Postives = 392/408 (96.08%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQNQ N+SS TPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNQSNVSSSTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEVSPNRSLWLTAFRGSLPTFL SQTQSSPST LD+TPS+SK
Sbjct: 61  LPRAQALLLQMASLASKLFEVSPNRSLWLTAFRGSLPTFLTSQTQSSPSTFLDSTPSSSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADY+RP+RVKT+   EN+SLCSTTVASQL+LSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYQRPRRVKTEGGAENESLCSTTVASQLRLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQ+EQMRASQLYNFA+LDVGLPKAVESTEKTIEPMIEPPAPV  DANPL+
Sbjct: 241 APLRGALPPAPQDEQMRASQLYNFANLDVGLPKAVESTEKTIEPMIEPPAPVRVDANPLS 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
           N+AAIQGLLPPNITVPSGWKPGMPVELP DLPMPPPGWKPGDPV LPP+DSLPV RVDEP
Sbjct: 301 NMAAIQGLLPPNITVPSGWKPGMPVELPGDLPMPPPGWKPGDPVRLPPMDSLPVTRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           Q LRPNALPGLHKPPEPIQVR VQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 Q-LRPNALPGLHKPPEPIQVRHVQLDILDQDDDSSDYTSDEGSSEDDD 407

BLAST of MC01g0504 vs. ExPASy TrEMBL
Match: A0A1S3BLP6 (Mediator of RNA polymerase II transcription subunit 4 OS=Cucumis melo OX=3656 GN=LOC103491209 PE=3 SV=1)

HSP 1 Score: 712 bits (1837), Expect = 2.79e-257
Identity = 378/408 (92.65%), Postives = 392/408 (96.08%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQNQ N+SS TPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNQSNVSSSTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEVSPNRSLWLTAFRGSLPTFL SQTQSSPST LD+TPS+SK
Sbjct: 61  LPRAQALLLQMASLASKLFEVSPNRSLWLTAFRGSLPTFLTSQTQSSPSTFLDSTPSSSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADY+RP+RVKT+   EN+SLCSTTVASQL+LSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYQRPRRVKTEGGAENESLCSTTVASQLRLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQ+EQMRASQLYNFA+LDVGLPKAVESTEKTIEPMIEPPAPV  DANPL+
Sbjct: 241 APLRGALPPAPQDEQMRASQLYNFANLDVGLPKAVESTEKTIEPMIEPPAPVRVDANPLS 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
           N+AAIQGLLPPNITVPSGWKPGMPVELP DLPMPPPGWKPGDPV LPP+DSLPV RVDEP
Sbjct: 301 NMAAIQGLLPPNITVPSGWKPGMPVELPGDLPMPPPGWKPGDPVRLPPMDSLPVTRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           Q LRPNALPGLHKPPEPIQVR VQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 Q-LRPNALPGLHKPPEPIQVRHVQLDILDQDDDSSDYTSDEGSSEDDD 407

BLAST of MC01g0504 vs. ExPASy TrEMBL
Match: A0A6J1HH73 (Mediator of RNA polymerase II transcription subunit 4 OS=Cucurbita moschata OX=3662 GN=LOC111463518 PE=3 SV=1)

HSP 1 Score: 703 bits (1814), Expect = 8.89e-254
Identity = 378/409 (92.42%), Postives = 390/409 (95.35%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQN  NLSSPTPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNPSNLSSPTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEV PNR+LWLTAFRGSLPTFLNSQTQS+PSTPLD+TPSTSK
Sbjct: 61  LPRAQALLLQMASLASKLFEVYPNRALWLTAFRGSLPTFLNSQTQSAPSTPLDSTPSTSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYE+RAKDAALRAFANKLKDAEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEMRAKDAALRAFANKLKDAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTT-VASQLKLSDILSYAHRISYTTFAPPEFGAG 240
           LFDDYADY+  +RVKT DDVEN+SLCSTT VASQLKLSDILSYAH+ISYTTFAPPEFGAG
Sbjct: 181 LFDDYADYQGHKRVKTSDDVENESLCSTTTVASQLKLSDILSYAHKISYTTFAPPEFGAG 240

Query: 241 QAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPL 300
           QAPLRGALPPAPQEEQMRASQLY FADLDVGLPK+VESTEKTIEPMIE PAPVPTDANPL
Sbjct: 241 QAPLRGALPPAPQEEQMRASQLYTFADLDVGLPKSVESTEKTIEPMIEAPAPVPTDANPL 300

Query: 301 ANLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDE 360
           A LA IQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPV RVDE
Sbjct: 301 A-LAGIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVARVDE 360

Query: 361 PQQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           PQ LR NALPGLH+P EPIQVR VQLDILDQDDDSSDY S+EGSSEDDD
Sbjct: 361 PQ-LRHNALPGLHRPQEPIQVRHVQLDILDQDDDSSDYASEEGSSEDDD 407

BLAST of MC01g0504 vs. ExPASy TrEMBL
Match: A0A0A0KXB7 (Mediator of RNA polymerase II transcription subunit 4 OS=Cucumis sativus OX=3659 GN=MED4 PE=3 SV=1)

HSP 1 Score: 702 bits (1813), Expect = 1.13e-253
Identity = 377/408 (92.40%), Postives = 389/408 (95.34%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTPPKLPPSQQSHHQNQPNLSSPTPSSTLLPLLPP 60
           MLQHQI QSPARLGL NPNSPSIQNPTPPKLPPSQQSHHQNQ NLSS TPSSTLLPLLPP
Sbjct: 1   MLQHQIVQSPARLGLTNPNSPSIQNPTPPKLPPSQQSHHQNQSNLSSSTPSSTLLPLLPP 60

Query: 61  LPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPTFLNSQTQSSPSTPLDATPSTSK 120
           LPRAQALLLQMA+LASKLFEVSPNRSLWLTAFRGSLPTFL SQTQSSPST LD+TPS+SK
Sbjct: 61  LPRAQALLLQMASLASKLFEVSPNRSLWLTAFRGSLPTFLTSQTQSSPSTLLDSTPSSSK 120

Query: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKDAEQVLDV 180
           EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLK+AEQVLDV
Sbjct: 121 EILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIRAKDAALRAFANKLKEAEQVLDV 180

Query: 181 LFDDYADYRRPQRVKTKDDVENDSLCSTTVASQLKLSDILSYAHRISYTTFAPPEFGAGQ 240
           LFDDYADY+RP+R KT+ D EN+SLCSTTVASQL+LSDILSYAHRISYTTFAPPEFGAGQ
Sbjct: 181 LFDDYADYQRPRRDKTEGDAENESLCSTTVASQLRLSDILSYAHRISYTTFAPPEFGAGQ 240

Query: 241 APLRGALPPAPQEEQMRASQLYNFADLDVGLPKAVESTEKTIEPMIEPPAPVPTDANPLA 300
           APLRGALPPAPQ+EQMRASQLYNFA+LDVGLPKAVESTEKTIEPMIEPPAPV  DANPLA
Sbjct: 241 APLRGALPPAPQDEQMRASQLYNFANLDVGLPKAVESTEKTIEPMIEPPAPVRPDANPLA 300

Query: 301 NLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMPPPGWKPGDPVPLPPLDSLPVPRVDEP 360
              AIQGLLPPNITVPSGWKPGMPVELP DLPMPPPGWKPGDPV LPP+DSLPV RVDEP
Sbjct: 301 ---AIQGLLPPNITVPSGWKPGMPVELPGDLPMPPPGWKPGDPVRLPPMDSLPVTRVDEP 360

Query: 361 QQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDSSDYTSDEGSSEDDD 408
           Q LRPNALPGLHKPPEPIQVR VQLDILDQDDDSSDYTSDEGSSEDDD
Sbjct: 361 Q-LRPNALPGLHKPPEPIQVRHVQLDILDQDDDSSDYTSDEGSSEDDD 404

BLAST of MC01g0504 vs. TAIR 10
Match: AT5G02850.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.1e-141
Identity = 286/434 (65.90%), Postives = 337/434 (77.65%), Query Frame = 0

Query: 1   MLQHQIAQSPARLGLNNPNSPSIQNPTP-----PKLPPSQQSHH---QNQPNL------- 60
           MLQHQI QSPARLGL  P SPS+QNPTP     P    S QS H   Q QPNL       
Sbjct: 1   MLQHQIVQSPARLGLTGPGSPSVQNPTPTRHGHPTSSSSSQSQHQQIQQQPNLLPSSTVA 60

Query: 61  -------SSPTPSSTLLPLLPPLPRAQALLLQMATLASKLFEVSPNRSLWLTAFRGSLPT 120
                  S+   SS LL LLPPLPRAQALL QMA L SKLF+VSPNR++WL+AFRGSLP+
Sbjct: 61  AASSASASAAVSSSALLSLLPPLPRAQALLQQMAVLTSKLFDVSPNRAIWLSAFRGSLPS 120

Query: 121 FLNSQTQSSPSTPLDATPSTSKEILSLFNVLQTQLFEAVAELQEILDLQDSKQKIAYEIR 180
           FL+S +   P    + +PS++KEILS FN LQTQLFEAV ELQEILDLQD+KQK+A EI+
Sbjct: 121 FLSSHSLPPPPPLENPSPSSTKEILSQFNSLQTQLFEAVTELQEILDLQDAKQKVAREIK 180

Query: 181 AKDAALRAFANKLKDAEQVLDVLFDDYADYRRPQRVK-TKDDVEND---SLCSTTVASQL 240
           +KD++L AFANKLKDAE+VLD+L DDY+DYR+P+R K  +DD +ND   S  STTV+SQL
Sbjct: 181 SKDSSLLAFANKLKDAERVLDMLVDDYSDYRKPKRSKIEEDDEDNDNESSSSSTTVSSQL 240

Query: 241 KLSDILSYAHRISYTTFAPPEFGAGQAPLRGALPPAPQEEQMRASQLYNFADLDVGLPKA 300
           KL DIL+YAH+ISYTTFAPPEFGAGQAPLRGALPPAPQ+EQMRASQLY FADLD+GLPK 
Sbjct: 241 KLKDILAYAHKISYTTFAPPEFGAGQAPLRGALPPAPQDEQMRASQLYTFADLDIGLPKT 300

Query: 301 VESTEKTIEPMIEPPAPVPTDANPLANLAAIQGLLPPNITVPSGWKPGMPVELPRDLPMP 360
           VE+ EK +E +IEPP P         +++AI  LLPPNI VPSGWKPGMPVELPRDLP+P
Sbjct: 301 VENMEKKVEALIEPPPPPEA-----MDISAIHNLLPPNIAVPSGWKPGMPVELPRDLPLP 360

Query: 361 PPGWKPGDPVPLPPLDSLPVPRVDEPQQLRPNALPGLHKPPEPIQVRPVQLDILDQDDDS 409
           PPGWKPGDPV LPPL+S+  PR ++ Q +RP+   GLH+PP+ IQVR VQLDIL +DDDS
Sbjct: 361 PPGWKPGDPVVLPPLESIAAPRAEDHQHMRPS--QGLHRPPDVIQVRAVQLDIL-EDDDS 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZ001.5e-14065.90Mediator of RNA polymerase II transcription subunit 4 OS=Arabidopsis thaliana OX... [more]
Q54D001.9e-0527.27Putative mediator of RNA polymerase II transcription subunit 4 OS=Dictyostelium ... [more]
Match NameE-valueIdentityDescription
XP_022143910.12.61e-283100.00mediator of RNA polymerase II transcription subunit 4 [Momordica charantia][more]
XP_038882754.15.65e-26494.12mediator of RNA polymerase II transcription subunit 4 [Benincasa hispida][more]
XP_008449288.15.76e-25792.65PREDICTED: mediator of RNA polymerase II transcription subunit 4 [Cucumis melo] ... [more]
XP_023518528.11.35e-25592.91mediator of RNA polymerase II transcription subunit 4 [Cucurbita pepo subsp. pep... [more]
XP_022963248.11.84e-25392.42mediator of RNA polymerase II transcription subunit 4 [Cucurbita moschata] >KAG7... [more]
Match NameE-valueIdentityDescription
A0A6J1CQ511.26e-283100.00Mediator of RNA polymerase II transcription subunit 4 OS=Momordica charantia OX=... [more]
A0A5A7TZC12.79e-25792.65Mediator of RNA polymerase II transcription subunit 4 OS=Cucumis melo var. makuw... [more]
A0A1S3BLP62.79e-25792.65Mediator of RNA polymerase II transcription subunit 4 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1HH738.89e-25492.42Mediator of RNA polymerase II transcription subunit 4 OS=Cucurbita moschata OX=3... [more]
A0A0A0KXB71.13e-25392.40Mediator of RNA polymerase II transcription subunit 4 OS=Cucumis sativus OX=3659... [more]
Match NameE-valueIdentityDescription
AT5G02850.11.1e-14165.90hydroxyproline-rich glycoprotein family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019258Mediator complex, subunit Med4PFAMPF10018Med4coord: 129..265
e-value: 2.1E-7
score: 30.8
IPR019258Mediator complex, subunit Med4PANTHERPTHR13208MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 4coord: 50..407
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 389..408
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..408
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..52
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 327..354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0504.1MC01g0504.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0070847 core mediator complex
cellular_component GO:0016592 mediator complex
molecular_function GO:0003712 transcription coregulator activity