MC01g0456 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0456
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	very-long-chain enoyl-CoA reductase-like
Location	MC01: 11136184 .. 11141058 (-)
RNA-Seq Expression	MC01g0456
Synteny	MC01g0456

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGACATAGCACAAATTTGTGTTGAATGTAATTTAATTTATGGTTCATTTTGTGCTCCGGGTGGGGTCCGCCAAACCGCCAACCGTTTCCCTCCGACCGACTTCGGCGGCAGATTGAACGTGAACAAACAAATTCACAAACGAACTGGGAGGTGGTCCACCTATTGACGCCATTAATCTCTCTACTCTCACCTTCATTTAATTTCTCTCTGCAACTCCGAATCCGCTCTCTGCTCATACTATAACCACTCTACAGCTTCTTCAACACTTGTAATCCAACCATGAAGGTCTCCATTGTTTCCAGGAGCGGTAGAGAAATCATCAGCGGCGGCCTTCAGATCGATGATTCGGTGAGCTTCTCGTTTTCGATTGTTCTTGGCTACATTATTGTTTATGCTCGTTTTTACGATCGGTTCTACTTTTAGTCGCGGGATTTTGTATGTTACTGTAGTGAATTGCGCAAGATTTTTTTGTTTTTTTCTGATGTATCTTTGTGCGATGGAGAGTCGAAAATCAGAATCTCGCCTTCTTCTTCTTCTTCTTTTAATTTTTAGATTTCTAATTCAGTGATTTTCTTGCGCTTGGCGATTTTTAGGCTACGGTGAGCGACTTGCAGGAGGCTATTCATCAGCGACGTAAGTCCTGACTCCACTATTTTGCATCAGTTTCTGCATAATTAGGCGTGTTACGTTTAGCATCTTTTTGCTGCAATACTGATTTTCGCAAAACTACTCCGTTAACAGCTGAGATTTAAATCACCTGATTTTACTCTATCATAGTGAAAATCCATCTCATTAGGAACGATCACAGAATTTATAGTGCAGCAAGGTCTCAAAGTTCTCGATTACGCCTCGGATGAGATAAAAAGGAGTTTAGTTCCGACTTGTGAGCATATATTGCTGTAGCTTTCTATTGTCTTTCTGGATTACTAAATAATGAATTGATACGATACTTGCTGGCAATTCCTTGTGTTTTTAATAGCTTTCAGTAGTTTCTTTACAGAGCACACTATATATATACCTTTAATTTTCCTTGGATATCTCTTTACTTATTTTTCTAGCTCAAGAAACTAAGATTTATGTCTCCAAAACTGAAGTATCTCTTATTGGCCTCTTATATTCTCATGTTCAACAGGCAGAAAATACTATCCTGCTAGACAGCGCCTTACCCTGCCTACCAAACCAGGGTCGAAAGAGAGGCCTGTTGTTCTTATTCCAAGCAAGAAACTCCAAGATTACTGCGATGGAAACTTGGATAAACTCACCATCACATTTAAGGACCTTGGTCCTCAGGTTTCTTATCGAACGCTCTTCTTGTTGGAGTATTTGGGGCCACTCCTTATCTATCCCACAATTTACTACTTTCCAGTCTATAAATTCTTTGGCTATAAGGAGGAACGTCATATCTACCCTGTCCAGACTTATGCCATGTTCTACTGGTGCCTCCATTACTCTAAGCGGATCTTGGAGACCTTCTTTGTTCATAGGTTTAGTCATGCAACTTCACCCTTGTCAAATGTCTTGCGCAACTGCGCTTACTATTGGAGTTTTGGAGCTCTCATAGCCTACTATGTAAACCACCCACTTTACACCCCAGTTGATGACCTCCAAATGAAGATTGGTTTTGGATTTGGATTAGTTTGTCAAATCTCTAACTTGTACTGCCATCTCATATTAAAGAATCTTAGAGACCCTAGTGGAAAGGGAGGCTACAAAATTCCACGTGGGTTCCTTTTCAATATTGTAACTTGTGCCAACTACACAACTGAGATTTATCAGTGGCTTGGGTTCAACATCGCGACACGAACTGTTGCAGGTTATATTTTTGTAATGGCGGCTATCTACATAATGAGCAAATGGGCGATTGCAAAACACCGCCGTTTGGAAAAGGTAATGATTAGCTGTTTGTCTCTTGTGTTCGTTCATATAAAATGCACTGCCCCCACTGAAATCTCAAAAGGCTTTTACTTTTACTGTGCCTAAAATTCCAATATATATGCTGGAACCTATAAATTTTATGTTTAATTTAGTGCACTTGGTCTTTAACTAATGAAGTCTGAGTCAACCCAATTGTAATTTTGAAGTAGCAGATCCTGTGTAGGGTGATCTGTGCTGTATAATCGATCTTTTGCCAACAATACTGTTCCATCAGGAACATATTTCTTTGGTTTCTTGTTATGAACTGTGCCGTCTAATCTATCTAGTATTATTTCCTCATTTGAATGTTTAAAATTAAATTTAAACGGGATCTATCTGGCTGCATCTACAGTTTTGTGGTTTTATTACTTTCTATATATTCTGAATGCCGTCTTCTCTGCAGCTTTTCGATGGGAAGGAAGGGAGGCCGAGGTATCCAAGAAGATGGGTGATTCTACCGCCATTTTTCTGAAAACAACAAAAGCCTTCACTTGGGGGAGAAAATAATCCTAAATCTGCAGTTGCAACTTGGCAAGCCAACATTGATGAAGCCCACGAACTTCCAGAAAATCACTGCTGTCGCGCTATTTAAGTATGTTGTTTCTTAATCGTTTTATTACAAGACCTTATATAGAAGAAATTCTCAGAGACAATTTACAAGGCTTGGTTTATTTATTTTTTAGGATGTAATCTTCATATTATATCTGAGTAATCTTTATTTTTCAAGTCTGAATTGCCTGACCTGAATTCATCCATCGTTATCACCATTCTGGTATTATATATACAATAACATAATGTAGAGATAATGTTGAATACCAAAAATCCTTAGTAGTTAGGGTACAAAAACTCAAGTTTATTCTGAAGTTCATATTTGATGTATATAATAATCTACAAAAACAGCAGCCAGCTTTAGCCTTTGAAACAACCTCCCGGCAGCTTGCGTTTTTAGCACTATACCTTAGAGAGAACTCTATCCTAGCACTCAATTCACTAGAAAAGAGTTCTATATGGTAATAAACTAGAAGCCAAATTCCCAATCCTAATTAGGAAATCATCATGAATTTGTCGAGTGGGTCGATCCCGATCCCCTATGTAGTTGCCCAGAAATTAGTCTGTCGCCCAATCCTACGTGGCAGTCCAAAGTTTTGATAGGCCCTTATTTCACCTCCCAACCCAGCAGTCACAATTACCAAGCCCCATGCCGTAGCAGGCACAGCATGGTGACCATGGCTGTTCCCAACATCAAACCAGTTCCAAGAGGAAGACCAGGCTGAAGATGATGGGTTGTTGATTGCAGCAGAAGAAATAGAAGGCGAATCAACATACTTGATAGAAGCTGAAGGTGAGGCTGAAGGTGAGGCTGAGGCTGAAGGTGATAAGCTGGGTGACTCACCAGTACTTTTAGAATCTAAGCGGGTAACTTGAGCTTGCTGCTGACCAGTACTTTTAGAATCCAAGCGGGTAATTTGAGCTTGCTGCGGCCCAGTACCTTTGGAATCTAAGCGGGTAATTTGAGCTTGCTGCGGCTCTTCGTCTGGAGGAGTTGAGGTATTGTTGTTGTTACTGTTGTTGTTGTTACTGTTGTTGTTGTTACTATTGTTGTTGTTGCTGTTGTTATTGTTGTTGCTGTTGTTCTCCCCAGCATCTTTGTTGTTGTTTTTCTTTGGTAGAGGTGGCAACTGTTTCTTACAGTTTGCAGTTGAGCTGTTTTCTTCTCGAGTAGGTGAGGAGCAGGCTGAAGGTGGCTGCGATGACGGGGCGCGCTTCGAATGTCTTTTGGAGTTCATTTGCGCCAATGGCGGTTCGCCGCTTATCATGCCAGGCCATGCGATTGCTACAGAAACATCTTTGCAAGGGAAGTGTTCATGACCTCTGGTTGCTATCAAACCTTTTTTCCCAGAACTTGGATGTCGTGGCTCCTCTCTCTTCCATACAAATACTTGAGAATCTTCACTTGCACATATCACATATTTCCCATCTTGACTGAACGAAGCTGTAAGTTGGCTGCTTGTGTTTCGGAAACCTGTAAATTTTTACAGGTTATATATATTCTAACCTTTCATATAGAGGCAAATTAGAACGATAACTTTAAAAATCTTGCTTTTGATTCTACTTTCAGGTGGACATATATATATAGTCTCTTCCTGATTACCTCTAAACCTCTGAGTTACTTCGGTGCCTTCCAAGATCCGAATTCGAGAATCAGCAGAAGTAACTAGCACTTCAGTAGGGCTCCCTGGAACAAACTGTTCAGGTACCAAGTTATTACAGCATATAAAAAAAGTACACAGGACTTATCTGGTATCGAGAGCCAATTTCTTTAGATTTACTCCTAAAAGACTCTTTAAATACACTACCATTTTATATATAATAGGTAGTAAAAAAGTTAATTGTTCAGGATTAAATTTCAGTGATCATTAAAAAAAAGTCATGATCGTCACTGATATTCACTAATTATTTGAATCAGTATTTTTTAGTCATCAAATAAAAAATACTGATTACAAACTCCCCAAACAAATAACCTTTTTACTCTATATTATATATAATATATGGTATTGTACTCACGAAGTTTTTTGAGTTAAATCTAAAGAAATAATCTAAAGAAATGAGCTCCCAATACCAGACCGTTGTACTTTTTAACTTAATTCACGTATAGAAAAATTAACAATGGAGGCTAGAAGAGAAGAACACATGATTACTTGGAAGCCAGTGATCTTTTTTGCATGATTCTTCTTTTTGCTTTGCACATCAACCTGGTACTTCTGTTCTAATTTGTAATCTATACTCCAAATAATATTGGTAAGTTTAACTAGAACAACATTAATTATTATTGTTATTATTATAGTAACTCGATTGGAGTTTAATATAGAGATGTGTATATATTCATACCCTCTATGCTGTACATTCGGCAACTCCCTTTATGTGAACCTATTATGGCACCCTGCAAAAAATACAAAGCA

mRNA sequence

GGGACATAGCACAAATTTGTGTTGAATGTAATTTAATTTATGGTTCATTTTGTGCTCCGGGTGGGGTCCGCCAAACCGCCAACCGTTTCCCTCCGACCGACTTCGGCGGCAGATTGAACGTGAACAAACAAATTCACAAACGAACTGGGAGGTGGTCCACCTATTGACGCCATTAATCTCTCTACTCTCACCTTCATTTAATTTCTCTCTGCAACTCCGAATCCGCTCTCTGCTCATACTATAACCACTCTACAGCTTCTTCAACACTTGTAATCCAACCATGAAGGTCTCCATTGTTTCCAGGAGCGGTAGAGAAATCATCAGCGGCGGCCTTCAGATCGATGATTCGGCTACGGTGAGCGACTTGCAGGAGGCTATTCATCAGCGACGCAGAAAATACTATCCTGCTAGACAGCGCCTTACCCTGCCTACCAAACCAGGGTCGAAAGAGAGGCCTGTTGTTCTTATTCCAAGCAAGAAACTCCAAGATTACTGCGATGGAAACTTGGATAAACTCACCATCACATTTAAGGACCTTGGTCCTCAGGTTTCTTATCGAACGCTCTTCTTGTTGGAGTATTTGGGGCCACTCCTTATCTATCCCACAATTTACTACTTTCCAGTCTATAAATTCTTTGGCTATAAGGAGGAACGTCATATCTACCCTGTCCAGACTTATGCCATGTTCTACTGGTGCCTCCATTACTCTAAGCGGATCTTGGAGACCTTCTTTGTTCATAGGTTTAGTCATGCAACTTCACCCTTGTCAAATGTCTTGCGCAACTGCGCTTACTATTGGAGTTTTGGAGCTCTCATAGCCTACTATGTAAACCACCCACTTTACACCCCAGTTGATGACCTCCAAATGAAGATTGGTTTTGGATTTGGATTAGTTTGTCAAATCTCTAACTTGTACTGCCATCTCATATTAAAGAATCTTAGAGACCCTAGTGGAAAGGGAGGCTACAAAATTCCACGTGGGTTCCTTTTCAATATTGTAACTTGTGCCAACTACACAACTGAGATTTATCAGTGGCTTGGGTTCAACATCGCGACACGAACTGTTGCAGGTTATATTTTTGTAATGGCGGCTATCTACATAATGAGCAAATGGGCGATTGCAAAACACCGCCGTTTGGAAAAGCTTTTCGATGGGAAGGAAGGGAGGCCGAGGTATCCAAGAAGATGGGTGATTCTACCGCCATTTTTCTGAAAACAACAAAAGCCTTCACTTGGGGGAGAAAATAATCCTAAATCTGCAGTTGCAACTTGGCAAGCCAACATTGATGAAGCCCACGAACTTCCAGAAAATCACTGCTGTCGCGCTATTTAAGTATGTTGTTTCTTAATCGTTTTATTACAAGACCTTATATAGAAGAAATTCTCAGAGACAATTTACAAGGCTTGGTTTATTTATTTTTTAGGATGTAATCTTCATATTATATCTGAGTAATCTTTATTTTTCAAGTCTGAATTGCCTGACCTGAATTCATCCATCGTTATCACCATTCTGGTATTATATATACAATAACATAATGTAGAGATAATGTTGAATACCAAAAATCCTTAGTAGTTAGGGTACAAAAACTCAAGTTTATTCTGAAGTTCATATTTGATGTATATAATAATCTACAAAAACAGCAGCCAGCTTTAGCCTTTGAAACAACCTCCCGGCAGCTTGCGTTTTTAGCACTATACCTTAGAGAGAACTCTATCCTAGCACTCAATTCACTAGAAAAGAGTTCTATATGGTAATAAACTAGAAGCCAAATTCCCAATCCTAATTAGGAAATCATCATGAATTTGTCGAGTGGGTCGATCCCGATCCCCTATGTAGTTGCCCAGAAATTAGTCTGTCGCCCAATCCTACGTGGCAGTCCAAAGTTTTGATAGGCCCTTATTTCACCTCCCAACCCAGCAGTCACAATTACCAAGCCCCATGCCGTAGCAGGCACAGCATGGTGACCATGGCTGTTCCCAACATCAAACCAGTTCCAAGAGGAAGACCAGGCTGAAGATGATGGGTTGTTGATTGCAGCAGAAGAAATAGAAGGCGAATCAACATACTTGATAGAAGCTGAAGGTGAGGCTGAAGGTGAGGCTGAGGCTGAAGGTGATAAGCTGGGTGACTCACCAGTACTTTTAGAATCTAAGCGGGTAACTTGAGCTTGCTGCTGACCAGTACTTTTAGAATCCAAGCGGGTAATTTGAGCTTGCTGCGGCCCAGTACCTTTGGAATCTAAGCGGGTAATTTGAGCTTGCTGCGGCTCTTCGTCTGGAGGAGTTGAGGTGAGGAGCAGGCTGAAGGTGGCTGCGATGACGGGGCGCGCTTCGAATGTCTTTTGGAGTTCATTTGCGCCAATGGCGGTTCGCCGCTTATCATGCCAGGCCATGCGATTGCTACAGAAACATCTTTGCAAGGGAAGTGTTCATGACCTCTGGTTGCTATCAAACCTTTTTTCCCAGAACTTGGATGTCGTGGCTCCTCTCTCTTCCATACAAATACTTGAGAATCTTCACTTGCACATATCACATATTTCCCATCTTGACTGAACGAAGCTGTGGACATATATATATAGTCTCTTCCTGATTACCTCTAAACCTCTGAGTTACTTCGGTGCCTTCCAAGATCCGAATTCGAGAATCAGCAGAAGTAACTAGCACTTCAGTAGGGCTCCCTGGAACAAACTGTTCAGGTACCAAGTTATTACAGCATATAAAAAAAGTACACAGGACTTATCTGGTATCGAGAGCCAATTTCTTTAGATTTACTCCTAAAAGACTCTTTAAATACACTACCATTTTATATATAATAGGTAGTAAAAAAGTTAATTGTTCAGGATTAAATTTCAGTGATCATTAAAAAAAAGTCATGATCGTCACTGATATTCACTAATTATTTGAATCAGTATTTTTTAGTCATCAAATAAAAAATACTGATTACAAACTCCCCAAACAAATAACCTTTTTACTCTATATTATATATAATATATGGTATTGTACTCACGAAGTTTTTTGAGTTAAATCTAAAGAAATAATCTAAAGAAATGAGCTCCCAATACCAGACCGTTGTACTTTTTAACTTAATTCACGTATAGAAAAATTAACAATGGAGGCTAGAAGAGAAGAACACATGATTACTTGGAAGCCAGTGATCTTTTTTGCATGATTCTTCTTTTTGCTTTGCACATCAACCTGGTACTTCTGTTCTAATTTGTAATCTATACTCCAAATAATATTGGTAAGTTTAACTAGAACAACATTAATTATTATTGTTATTATTATAGTAACTCGATTGGAGTTTAATATAGAGATGTGTATATATTCATACCCTCTATGCTGTACATTCGGCAACTCCCTTTATGTGAACCTATTATGGCACCCTGCAAAAAATACAAAGCA

Coding sequence (CDS)

ATGAAGGTCTCCATTGTTTCCAGGAGCGGTAGAGAAATCATCAGCGGCGGCCTTCAGATCGATGATTCGGCTACGGTGAGCGACTTGCAGGAGGCTATTCATCAGCGACGCAGAAAATACTATCCTGCTAGACAGCGCCTTACCCTGCCTACCAAACCAGGGTCGAAAGAGAGGCCTGTTGTTCTTATTCCAAGCAAGAAACTCCAAGATTACTGCGATGGAAACTTGGATAAACTCACCATCACATTTAAGGACCTTGGTCCTCAGGTTTCTTATCGAACGCTCTTCTTGTTGGAGTATTTGGGGCCACTCCTTATCTATCCCACAATTTACTACTTTCCAGTCTATAAATTCTTTGGCTATAAGGAGGAACGTCATATCTACCCTGTCCAGACTTATGCCATGTTCTACTGGTGCCTCCATTACTCTAAGCGGATCTTGGAGACCTTCTTTGTTCATAGGTTTAGTCATGCAACTTCACCCTTGTCAAATGTCTTGCGCAACTGCGCTTACTATTGGAGTTTTGGAGCTCTCATAGCCTACTATGTAAACCACCCACTTTACACCCCAGTTGATGACCTCCAAATGAAGATTGGTTTTGGATTTGGATTAGTTTGTCAAATCTCTAACTTGTACTGCCATCTCATATTAAAGAATCTTAGAGACCCTAGTGGAAAGGGAGGCTACAAAATTCCACGTGGGTTCCTTTTCAATATTGTAACTTGTGCCAACTACACAACTGAGATTTATCAGTGGCTTGGGTTCAACATCGCGACACGAACTGTTGCAGGTTATATTTTTGTAATGGCGGCTATCTACATAATGAGCAAATGGGCGATTGCAAAACACCGCCGTTTGGAAAAGCTTTTCGATGGGAAGGAAGGGAGGCCGAGGTATCCAAGAAGATGGGTGATTCTACCGCCATTTTTCTGA

Protein sequence

MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPVVLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFGYKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIAYYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYPRRWVILPPFF

Homology

[Close]

BLAST of MC01g0456 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 4.5e-145
Identity = 233/309 (75.40%), Postives = 268/309 (86.73%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGRE++   L + DSATV+DLQEA H+R +K+YP+RQRLTLP  PGSK++PV
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L++YCDGN + LT+ FKDLG QVSYRTLF  EYLGPLLIYP  YYFPVYKF G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           Y E+  I+PVQTYAM+YWC HY KRILETFFVHRFSHATSP+ NV RNCAYYWSFGA IA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGFGFGLVCQ++N YCH++LKNLRDPSG GGY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+T+AGY+F+  A  IM+ WA+ KH RL K+FDGK+G+P+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

Query: 301 RRWVILPPF 310
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.3e-54
Identity = 124/309 (40.13%), Postives = 176/309 (56.96%), Query Frame = 0


Query: 3   VSIVS-RSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPVV 62
           + +VS RS +E+  G      S TV +L++ I  + R     R RL +P+K  + + P  
Sbjct: 4   IKVVSQRSKKEV--GSFSTSSSTTVGELKKQISSKTR-LGTERIRLAVPSK--TSKLPNA 63

Query: 63  LIPSKKLQDYCDGNLD-KLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 122
                K  D    ++    T+ FKDLGPQ+S+  +F+ EY GPL +YP I+YF     +G
Sbjct: 64  FEALGKDSDLVSKHVGADSTLYFKDLGPQISWSLVFICEYAGPLFVYP-IFYFLSNLIYG 123

Query: 123 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 182
               +     Q  A+  + LHY KRI ET FVHRFSH T P+ N+ +NC+YYW   A+++
Sbjct: 124 TDSPKSF--AQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVS 183

Query: 183 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 242
           Y+VNHPLYT     ++ +G G  ++ ++ N  CH+ L+NLR P+G    KIPRG LF  V
Sbjct: 184 YFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFV 243

Query: 243 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 302
           +C NYT EI  W+GF+I T+T+  +IF +     M  WA+ KHRR  K F  K     YP
Sbjct: 244 SCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDK-----YP 298

Query: 303 RRWVILPPF 310
           +   IL PF
Sbjct: 304 KSRKILIPF 298

[Close]

BLAST of MC01g0456 vs. ExPASy Swiss-Prot
Match: Q9N5Y2 (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=6239 GN=art-1 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 2.5e-47
Identity = 108/298 (36.24%), Postives = 163/298 (54.70%), Query Frame = 0


Query: 20  IDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPVVLIPSKKLQDYCDGNLDKL 79
           I  S T+  +++ I Q++ K    RQ L +      K +P  L   +KL D    +  K 
Sbjct: 23  ISGSETIKAIKKRIAQKKLKLTEERQALRVE----PKGKP--LADDQKLSD-LGLSSQKA 82

Query: 80  TITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFGYKEERHIYPVQTYAMFYWC 139
            +  +DLGPQ++++T+F+ EY GPL +YP  Y  P + +        ++P    A F W 
Sbjct: 83  VLYVRDLGPQIAWKTVFMAEYAGPLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWS 142

Query: 140 LHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIAYYVNHPLYTP--VDDLQMK 199
            HY+KR+ ET F+HRF ++T P  N+++NC+YYW F A +AY+VNHPL+TP    DLQ+ 
Sbjct: 143 FHYAKRLFETQFIHRFGNSTMPQFNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVY 202

Query: 200 IGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPR------GFLFNIVTCANYTTEIYQ 259
            G    ++ +  NL  H++L+NLR P+G    +IP+        LFN V+C NYT E+  
Sbjct: 203 FGLAGFVISEFGNLSIHILLRNLR-PAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVAS 262

Query: 260 WLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYPRRWVILPPF 310
           W+ F+I  +++   IF  A    M+ WA  KHR   K F      P YP+    + PF
Sbjct: 263 WIFFSIMVQSLPAIIFTTAGFAQMAIWAQGKHRNYLKEF------PDYPKNRKAIVPF 306

[Close]

BLAST of MC01g0456 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.7e-46
Identity = 122/324 (37.65%), Postives = 172/324 (53.09%), Query Frame = 0


Query: 2   KVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPVV 61
           +V I+    RE +    +++  AT+++++    +   ++YPARQ L L  K         
Sbjct: 5   EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRLDPK--------- 64

Query: 62  LIPSKKLQDYCDGNLDKL------TITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPV 121
               K L+D  +  L KL      T+ F+DLG Q+S+ T+FL EY GPL IY  ++YF V
Sbjct: 65  ---GKSLKD--EDVLQKLPVGTTATLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRV 124

Query: 122 YKFFGYK----EERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAY 181
              +G+K      RH   V   A      HY KR+LET FVHRFSH T PL N+ +NC Y
Sbjct: 125 PFIYGHKYDFTSSRH--TVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTY 184

Query: 182 YWSFGALIAYYVNHPLYTP--VDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGY 241
           YW F A +AYY+NHPLYTP      Q+K+     ++CQ+ N   H+ L++LR P+G    
Sbjct: 185 YWGFAAWMAYYINHPLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTR 244

Query: 242 KIPR------GFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKH 301
           KIP        +LF +V+C NYT E+  W+GF I T+ +   +F +     M+ WA  KH
Sbjct: 245 KIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKH 304

Query: 302 RRLEKLFDGKEGRPRYPRRWVILP 308
           R   K F     R   P R  I+P
Sbjct: 305 RSYLKEF-----RDYPPLRMPIIP 305

[Close]

BLAST of MC01g0456 vs. ExPASy Swiss-Prot
Match: Q64232 (Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.3e-46
Identity = 120/307 (39.09%), Postives = 164/307 (53.42%), Query Frame = 0


Query: 19  QIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPVVLIPSKKLQDYCDGNLDK 78
           +++  AT+S+++    +   ++YPARQ L L  K             K L+D  +  L K
Sbjct: 22  KVEPQATISEIKTLFTKTHPQWYPARQSLRLDPK------------GKSLKD--EDVLQK 81

Query: 79  L------TITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFGYK----EERHIY 138
           L      T+ F+DLG Q+S+ T+FL EY GPL IY  ++YF V   +G K      RH  
Sbjct: 82  LPVGTTATLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGRKYDFTSSRH-- 141

Query: 139 PVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIAYYVNHPLY 198
            V   A      HY KR+LET FVHRFSH T PL N+ +NC YYW F A +AYY+NHPLY
Sbjct: 142 TVVHLACMCHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINHPLY 201

Query: 199 TP--VDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPR------GFLFNIV 258
           TP      Q+K+     ++CQ+ N   H+ L++LR P+G    KIP        +LF +V
Sbjct: 202 TPPTYGVQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTKNPFTWLFLLV 261

Query: 259 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 308
           +C NYT E+  W+GF I T+ V   +F +     M+ WA  KHR   K F     R   P
Sbjct: 262 SCPNYTYEVGSWIGFAIMTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEF-----RDYPP 305

[Close]

BLAST of MC01g0456 vs. NCBI nr
Match: XP_022143915.1 (very-long-chain enoyl-CoA reductase-like [Momordica charantia])

HSP 1 Score: 641 bits (1654), Expect = 2.29e-232
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV
Sbjct: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG
Sbjct: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA
Sbjct: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300

Query: 301 RRWVILPPFF 310
           RRWVILPPFF
Sbjct: 301 RRWVILPPFF 310

[Close]

BLAST of MC01g0456 vs. NCBI nr
Match: XP_038877634.1 (very-long-chain enoyl-CoA reductase-like [Benincasa hispida])

HSP 1 Score: 601 bits (1549), Expect = 2.33e-216
Identity = 283/310 (91.29%), Postives = 301/310 (97.10%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV+I+SRSGREII+GGLQIDDSATVSDLQEAIHQRRRKYYP+RQRLTLP KPGSKERP+
Sbjct: 1   MKVTILSRSGREIITGGLQIDDSATVSDLQEAIHQRRRKYYPSRQRLTLPAKPGSKERPI 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VLIPSKKLQDYCDGNLD L+ITFKDLG QVSYRTLFLLEYLGPLLIYP  YYFPVYKFFG
Sbjct: 61  VLIPSKKLQDYCDGNLDNLSITFKDLGRQVSYRTLFLLEYLGPLLIYPMFYYFPVYKFFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           Y++ERHIYPVQTYAM+YWCLHYSKRI+ETFFVHRFSH TSPLSNV+RNC YYWSFGALI 
Sbjct: 121 YQDERHIYPVQTYAMYYWCLHYSKRIMETFFVHRFSHGTSPLSNVVRNCMYYWSFGALIG 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV+DLQMKIGFGFGL+CQISNLYCHLILKNLRDPSG+GGYKIPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVNDLQMKIGFGFGLLCQISNLYCHLILKNLRDPSGEGGYKIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+TVAGYIFV+AA YIM+KWAIAKHRRLEKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFVIAATYIMTKWAIAKHRRLEKLFDGKEGRPKYP 300

Query: 301 RRWVILPPFF 310
           RRWVILPPFF
Sbjct: 301 RRWVILPPFF 310

[Close]

BLAST of MC01g0456 vs. NCBI nr
Match: TXG58604.1 (hypothetical protein EZV62_016433 [Acer yangbiense])

HSP 1 Score: 535 bits (1378), Expect = 2.68e-190
Identity = 246/309 (79.61%), Postives = 275/309 (89.00%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGREI+ GGL + DSATVSDLQE IH R +KYYP+RQRLTLP  PGSKERP 
Sbjct: 1   MKVTVVSRSGREIVKGGLDLHDSATVSDLQETIHGRCKKYYPSRQRLTLPLPPGSKERPT 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL P KK+QDYCDGNLD LT+TFKDLGPQVSYRTLF  EY GPL+IYP  YYFPVYK FG
Sbjct: 61  VLNPKKKIQDYCDGNLDNLTVTFKDLGPQVSYRTLFFWEYFGPLVIYPIFYYFPVYKLFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           Y+ ER I+PVQTYAM+YWC HY KRI+ETFFVHRFSHATSPL+NV RNCAYYWSFG++IA
Sbjct: 121 YEGERAIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLANVFRNCAYYWSFGSVIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV+DLQMKIGFGFG++CQISN YCHLILKNLR   G  GY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVNDLQMKIGFGFGIICQISNFYCHLILKNLRSTDGSSGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+T+AGY F++ A +IM+ WA+AKHRRL+KLFDGKEGR +YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYTFLVVATFIMTNWALAKHRRLKKLFDGKEGRTKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. NCBI nr
Match: KAE7996787.1 (hypothetical protein FH972_001478 [Carpinus fangiana])

HSP 1 Score: 532 bits (1371), Expect = 3.12e-189
Identity = 243/309 (78.64%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKVS+VSRSG+E++ GGL   DSATV+DLQEAIH+R RK+YP+RQRLTLP +PGSKE+P+
Sbjct: 1   MKVSVVSRSGKELVKGGLDFSDSATVADLQEAIHRRTRKFYPSRQRLTLPLQPGSKEKPI 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L+DYC GNLD LT+ FKDLGPQVSYRTLF  EYLGPL++YP  YYFPVY++FG
Sbjct: 61  VLNYKKSLEDYCHGNLDHLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQYFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YKEER I PVQTYA++YWC HY KRI+ETFFVHRFSHATSPLSNV RNCAYYWSFGA IA
Sbjct: 121 YKEERVILPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGF FGL+CQ+SN YCHL+L+NLR P G GGY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFAFGLLCQVSNFYCHLMLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+TVAGYIF++ A  IM+ WA+AKHRRL+KLFDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAAAIMTNWALAKHRRLKKLFDGKDGRPKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. NCBI nr
Match: XP_021295484.1 (very-long-chain enoyl-CoA reductase [Herrania umbratica])

HSP 1 Score: 531 bits (1369), Expect = 6.29e-189
Identity = 242/309 (78.32%), Postives = 278/309 (89.97%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGRE+I GGL++ DSATV+DLQEAIH+R +K+YP+RQRLTLP  PGSKERPV
Sbjct: 1   MKVTLVSRSGREVIKGGLELHDSATVADLQEAIHKRTKKFYPSRQRLTLPVPPGSKERPV 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L+DYCDGN + +T+ FKDLGPQVSYRTLF  EYLGPL++YP  YYFPVY+FFG
Sbjct: 61  VLNCKKSLKDYCDGNQNNITVLFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YK ER I+PVQTYA++YWC HY KRI+ETFF+HRFSHATSPLSNV RNCAYYW+FG+ IA
Sbjct: 121 YKVERVIHPVQTYALYYWCFHYFKRIMETFFIHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGFGFGLVCQ++N YCH+ILKNLR P G GGY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQLANSYCHIILKNLRSPDGSGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+TVAGY+F++ A  IM+ WA+AKHRRL+KLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYVFLVVATSIMTNWALAKHRRLKKLFDGKEGRPKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. ExPASy TrEMBL
Match: A0A6J1CQ63 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111013716 PE=3 SV=1)

HSP 1 Score: 641 bits (1654), Expect = 1.11e-232
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV
Sbjct: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG
Sbjct: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA
Sbjct: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300

Query: 301 RRWVILPPFF 310
           RRWVILPPFF
Sbjct: 301 RRWVILPPFF 310

[Close]

BLAST of MC01g0456 vs. ExPASy TrEMBL
Match: A0A5C7HP47 (S5A_REDUCTASE domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_016433 PE=3 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 1.30e-190
Identity = 246/309 (79.61%), Postives = 275/309 (89.00%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGREI+ GGL + DSATVSDLQE IH R +KYYP+RQRLTLP  PGSKERP 
Sbjct: 1   MKVTVVSRSGREIVKGGLDLHDSATVSDLQETIHGRCKKYYPSRQRLTLPLPPGSKERPT 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL P KK+QDYCDGNLD LT+TFKDLGPQVSYRTLF  EY GPL+IYP  YYFPVYK FG
Sbjct: 61  VLNPKKKIQDYCDGNLDNLTVTFKDLGPQVSYRTLFFWEYFGPLVIYPIFYYFPVYKLFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           Y+ ER I+PVQTYAM+YWC HY KRI+ETFFVHRFSHATSPL+NV RNCAYYWSFG++IA
Sbjct: 121 YEGERAIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLANVFRNCAYYWSFGSVIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV+DLQMKIGFGFG++CQISN YCHLILKNLR   G  GY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVNDLQMKIGFGFGIICQISNFYCHLILKNLRSTDGSSGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+T+AGY F++ A +IM+ WA+AKHRRL+KLFDGKEGR +YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYTFLVVATFIMTNWALAKHRRLKKLFDGKEGRTKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. ExPASy TrEMBL
Match: A0A5N6QC86 (S5A_REDUCTASE domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_001478 PE=3 SV=1)

HSP 1 Score: 532 bits (1371), Expect = 1.51e-189
Identity = 243/309 (78.64%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKVS+VSRSG+E++ GGL   DSATV+DLQEAIH+R RK+YP+RQRLTLP +PGSKE+P+
Sbjct: 1   MKVSVVSRSGKELVKGGLDFSDSATVADLQEAIHRRTRKFYPSRQRLTLPLQPGSKEKPI 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L+DYC GNLD LT+ FKDLGPQVSYRTLF  EYLGPL++YP  YYFPVY++FG
Sbjct: 61  VLNYKKSLEDYCHGNLDHLTVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQYFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YKEER I PVQTYA++YWC HY KRI+ETFFVHRFSHATSPLSNV RNCAYYWSFGA IA
Sbjct: 121 YKEERVILPVQTYALYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGF FGL+CQ+SN YCHL+L+NLR P G GGY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFAFGLLCQVSNFYCHLMLRNLRSPDGNGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+TVAGYIF++ A  IM+ WA+AKHRRL+KLFDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAAAIMTNWALAKHRRLKKLFDGKDGRPKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. ExPASy TrEMBL
Match: A0A6J1BAL0 (very-long-chain enoyl-CoA reductase OS=Herrania umbratica OX=108875 GN=LOC110425038 PE=3 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 3.05e-189
Identity = 242/309 (78.32%), Postives = 278/309 (89.97%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGRE+I GGL++ DSATV+DLQEAIH+R +K+YP+RQRLTLP  PGSKERPV
Sbjct: 1   MKVTLVSRSGREVIKGGLELHDSATVADLQEAIHKRTKKFYPSRQRLTLPVPPGSKERPV 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L+DYCDGN + +T+ FKDLGPQVSYRTLF  EYLGPL++YP  YYFPVY+FFG
Sbjct: 61  VLNCKKSLKDYCDGNQNNITVLFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YK ER I+PVQTYA++YWC HY KRI+ETFF+HRFSHATSPLSNV RNCAYYW+FG+ IA
Sbjct: 121 YKVERVIHPVQTYALYYWCFHYFKRIMETFFIHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGFGFGLVCQ++N YCH+ILKNLR P G GGY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQLANSYCHIILKNLRSPDGSGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+TVAGY+F++ A  IM+ WA+AKHRRL+KLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYVFLVVATSIMTNWALAKHRRLKKLFDGKEGRPKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. ExPASy TrEMBL
Match: B9SXZ3 (Synaptic glycoprotein SC2, putative OS=Ricinus communis OX=3988 GN=RCOM_1206840 PE=3 SV=1)

HSP 1 Score: 531 bits (1369), Expect = 3.05e-189
Identity = 241/309 (77.99%), Postives = 276/309 (89.32%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGRE+I GGL ++DSATV+DLQEAIH+R +K+YP+RQRLTLP  PGSK+RP 
Sbjct: 1   MKVTVVSRSGREVIKGGLDLNDSATVADLQEAIHKRTKKFYPSRQRLTLPLPPGSKDRPT 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L+DYCDGN D +T+ FKDLGPQVSYRTLF  EYLGPL++YP  Y+FPVYK+FG
Sbjct: 61  VLNYKKSLKDYCDGNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPVFYFFPVYKYFG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           YKEER I+PVQTYAM+YWC HY KRI+ETFFVHRFSHATSPLSNV RNCAYYW+FG+ IA
Sbjct: 121 YKEERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWTFGSYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGFGFGL+CQI+N YCH++LKNLR P G GGY+IP GFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFGFGLICQIANFYCHILLKNLRSPDGSGGYQIPHGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+TVAGYIF++ A  IM+ WA+AKHRRL KLFDGK+GRP+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTVAGYIFLVVAASIMTNWALAKHRRLRKLFDGKDGRPKYP 300

Query: 301 RRWVILPPF 309
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 515.4 bits (1326), Expect = 3.2e-146
Identity = 233/309 (75.40%), Postives = 268/309 (86.73%), Query Frame = 0


Query: 1   MKVSIVSRSGREIISGGLQIDDSATVSDLQEAIHQRRRKYYPARQRLTLPTKPGSKERPV 60
           MKV++VSRSGRE++   L + DSATV+DLQEA H+R +K+YP+RQRLTLP  PGSK++PV
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  VLIPSKKLQDYCDGNLDKLTITFKDLGPQVSYRTLFLLEYLGPLLIYPTIYYFPVYKFFG 120
           VL   K L++YCDGN + LT+ FKDLG QVSYRTLF  EYLGPLLIYP  YYFPVYKF G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSPLSNVLRNCAYYWSFGALIA 180
           Y E+  I+PVQTYAM+YWC HY KRILETFFVHRFSHATSP+ NV RNCAYYWSFGA IA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYTPVDDLQMKIGFGFGLVCQISNLYCHLILKNLRDPSGKGGYKIPRGFLFNIV 240
           YYVNHPLYTPV DLQMKIGFGFGLVCQ++N YCH++LKNLRDPSG GGY+IPRGFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWLGFNIATRTVAGYIFVMAAIYIMSKWAIAKHRRLEKLFDGKEGRPRYP 300
           TCANYTTEIYQWLGFNIAT+T+AGY+F+  A  IM+ WA+ KH RL K+FDGK+G+P+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

Query: 301 RRWVILPPF 310
           RRWVILPPF
Sbjct: 301 RRWVILPPF 309

[Close]

BLAST of MC01g0456 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 79.7 bits (195), Expect = 4.5e-15
Identity = 60/210 (28.57%), Postives = 99/210 (47.14%), Query Frame = 0


Query: 102 GPLLIYPTIYYFPVYKFFGYKEERHIYPVQTYAMFYWCLHYSKRILETFFVHRFSHATSP 161
           G LL+Y   +      FF    +   + +   A+    LH+ KR+ E  F+H++S   + 
Sbjct: 68  GMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSAL---ALHFFKRVFEVLFIHKYSGGMA- 127

Query: 162 LSNVLRNCAYYWSFGALIAYYVNHPLYTPVDDLQMKI-GFGFGLVCQISNLYCHLILKNL 221
           + + L   + Y+S  AL+ Y  N  L        MK+ G    +V  + NLY H++L  L
Sbjct: 128 IDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLAKL 187

Query: 222 RDPSGKGGYKIPRGFLFNIVTCANYTTEIYQWLGFNIATRTVAGYIFVMAA-IYIMSKWA 281
           R   GK  YKIP+G LF+I+ C +Y  EI  +  F + ++T+  + F M   +Y++ +  
Sbjct: 188 RKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGRSY 247

Query: 282 IAKHRRLEKLFDGKEGRPRYPRRWVILPPF 310
             +   L K  D       +P+    L PF
Sbjct: 248 ATRTWYLSKFDD-------FPKHIKALIPF 266

[Close]

BLAST of MC01g0456 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 50.1 bits (118), Expect = 3.8e-06
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0


Query: 200 FGFGLVCQISNLYCHL----ILKNLRDPSGKGGYKIPRGFLFNIVTCANYTTEIYQWLGF 259
           F  G+V  I+ +Y ++     L  L+    +GGY IPRG  F +V+C NY  E  +WLG+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLK-KENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 260 NIATRTVAG---YIFVMAAIY----IMSKWAIAKHR 285
            + T + AG   +++  + ++       KW IAK +
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKFK 247

The following BLAST results are available for this feature:

BLAST of MC01g0456 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U2	4.5e-145	75.40	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ...	[more]
Q55C17	6.3e-54	40.13	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
Q9N5Y2	2.5e-47	36.24	Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=...	[more]
Q9NZ01	3.7e-46	37.65	Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1	[more]
Q64232	6.3e-46	39.09	Very-long-chain enoyl-CoA reductase OS=Rattus norvegicus OX=10116 GN=Tecr PE=1 S...	[more]

BLAST of MC01g0456 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143915.1	2.29e-232	100.00	very-long-chain enoyl-CoA reductase-like [Momordica charantia]	[more]
XP_038877634.1	2.33e-216	91.29	very-long-chain enoyl-CoA reductase-like [Benincasa hispida]	[more]
TXG58604.1	2.68e-190	79.61	hypothetical protein EZV62_016433 [Acer yangbiense]	[more]
KAE7996787.1	3.12e-189	78.64	hypothetical protein FH972_001478 [Carpinus fangiana]	[more]
XP_021295484.1	6.29e-189	78.32	very-long-chain enoyl-CoA reductase [Herrania umbratica]	[more]

BLAST of MC01g0456 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQ63	1.11e-232	100.00	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A5C7HP47	1.30e-190	79.61	S5A_REDUCTASE domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_0...	[more]
A0A5N6QC86	1.51e-189	78.64	S5A_REDUCTASE domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_...	[more]
A0A6J1BAL0	3.05e-189	78.32	very-long-chain enoyl-CoA reductase OS=Herrania umbratica OX=108875 GN=LOC110425...	[more]
B9SXZ3	3.05e-189	77.99	Synaptic glycoprotein SC2, putative OS=Ricinus communis OX=3988 GN=RCOM_1206840 ...	[more]

BLAST of MC01g0456 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55360.1	3.2e-146	75.40	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	4.5e-15	28.57	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	3.8e-06	32.29	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 159..309 e-value: 7.5E-27 score: 94.2
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 199..280 score: 21.416002
None	No IPR available	GENE3D	3.10.20.90		coord: 1..86 e-value: 1.3E-6 score: 30.0
None	No IPR available	PANTHER	PTHR10556:SF28	SC2	coord: 1..310
None	No IPR available	CDD	cd01801	Ubl_TECR_like	coord: 2..86 e-value: 1.59185E-20 score: 81.949
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 1..310

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0456.1	MC01g0456.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0019166	trans-2-enoyl-CoA reductase (NADPH) activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors