MC01g0404 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g0404 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 2.0e-189
Identity = 361/528 (68.37%), Postives = 414/528 (78.41%), Query Frame = 0

Query: 27  IEEHGRESSSSSDFLTSETTGLEEHSHSGS---DDSSSPSSMSWPTRKPEVPNSNGADKG 86
           +EE GRESS  S    SETT  EE   S S   D ++SP S  W                
Sbjct: 11  VEEKGRESSCCS----SETTRQEEEEQSPSCTEDFTASPVSSRWSV-------------- 70

Query: 87  GKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATL 146
            KN    +K  +  S  SE+EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLCATL
Sbjct: 71  -KNIDGEKKKIRSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATL 130

Query: 147 FGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTPTWQTFPDGSKLEVMTCRPRSDLY 206
           FGQLWRLEPLP EKK MWRREM+WLLCVSD IVE+TPTWQTFPDG+KLE+MTCRPRSDLY
Sbjct: 131 FGQLWRLEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLY 190

Query: 207 VNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET--DGSSSFRGALQRQEEKWWLPV 266
           VNLPALRKLDNMLLEILDSFE+ EFWYVDQGI+AHE+  DGSSSFR + QRQE+KWWLPV
Sbjct: 191 VNLPALRKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPV 250

Query: 267 PRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLADLDIPEPYLEALPKNGRACLG 326
           PRV PGGL E+SR+QLQHKR+ TNQILKAAMAIN+ TLAD++IPE YLE+LP+ GR+CLG
Sbjct: 251 PRVSPGGLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLG 310

Query: 327 DLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEASIYVWQKRINSKPATNSTKHSS 386
           DLIYRYISSDQFSPECLLDCL++SSEHQA EI NRVE+SIY+W KR NSKPATN     +
Sbjct: 311 DLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN-----T 370

Query: 387 KSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKSILE 446
           K+SWEMVKELMVD++K EL+A+RAE+LLL LKQRFPGLPQT LDMSKIQYNKD+GKSILE
Sbjct: 371 KTSWEMVKELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILE 430

Query: 447 SYSRVLESLAFNVVARIDDLLYVDDLTKH-SDQLSSISKLGVISHKNSPLHFSVPISGTP 506
           SYSRVLESLAFN+VARIDDLL+VDDLT+H SDQ+   + LG  +  ++P   +VP+S   
Sbjct: 431 SYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIP--TTLG-NNGNDAPKSIAVPVSN-- 490

Query: 507 YKTPFATPSFSPGASPAKGERS--PFFSGTKIPQRGFGVKKVLTDYLS 547
               + TPS+SP     +   +  P  S  KIP     VK+VLT Y++
Sbjct: 491 ----YTTPSYSPSKQELRSSITVPPSPSRFKIPHSS-SVKRVLTAYVT 504

BLAST of MC01g0404 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 7.5e-173
Identity = 327/557 (58.71%), Postives = 406/557 (72.89%), Query Frame = 0

Query: 15  HIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSSPSSMSWPTRKPEV 74
           ++ P  E +  L E   R S+S + + +S TT +     S S        + WP RK   
Sbjct: 17  NLTPSMEDI--LTESKERSSTSGASYESSSTTTVA----SSSPPPPPSQILGWPIRKASF 76

Query: 75  PNSNGADKGGKNHV-----DNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTA 134
              N  +    +H      D+   + K  +S+++EMMKERF+KLLLGEDMSG G GVCTA
Sbjct: 77  -RKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTA 136

Query: 135 LAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTPTWQTFPDGS 194
           LAISNAITNLCAT+FGQLWRLEPL  EKK MWRREM+W+L VSD IVELTP+ QT+PDG+
Sbjct: 137 LAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGN 196

Query: 195 KLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHETDGSSSFRGA 254
           K EVMTCRPR DL++NLPALRKLDNMLL+IL SF+  EFWYVDQGI+A E DGS+SFR  
Sbjct: 197 KFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRK 256

Query: 255 LQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLADLDIPEPYL 314
           +QRQEEKWWLPVPR+ P GL E++R +L HKRE   QILKAAMAIN+  L ++D+P+ YL
Sbjct: 257 IQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYL 316

Query: 315 EALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEASIYVWQKRIN 374
           E LPKNGR+CLGD+IYRY++SD+FS E LLDCL++SSEH A +I NRVEASIYVW++R+ 
Sbjct: 317 ETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQ 376

Query: 375 SKPATNSTKHSS--KSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMS 434
           +K   N+   S+  K +WEMVKELM   +KR LL ER+ETLL CLKQRFP L QT+LD+S
Sbjct: 377 TKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDIS 436

Query: 435 KIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQ---LSSISKLGVIS 494
           KIQ+NKD+GKSILESYSR LESLA N++ARIDDLLYVDDLTK SD    LSS +   +I+
Sbjct: 437 KIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIA 496

Query: 495 HKN-SPLHFSVPISGTPYKTPFA-TPSFSPG-ASPAKGERSPFFSGTK--------IPQR 551
           HK   PL + +  SGTPY+T F+ TP FSP   SP KGER   +S           +P R
Sbjct: 497 HKKVVPLPYLISASGTPYRTSFSTTPGFSPSMISPKKGERRTPYSSKDTNKIIEKGLPSR 556

BLAST of MC01g0404 vs. ExPASy Swiss-Prot
Match: Q9M056 (Rop guanine nucleotide exchange factor 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF6 PE=2 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 3.2e-139
Identity = 299/584 (51.20%), Postives = 394/584 (67.47%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           ME++     EG       ++ +  LI+     ++ SS  L+S ++G+   S   S    S
Sbjct: 1   MEDNSCIGFEGSKRFGETSKRIIGLIDSVTESTTDSS--LSSSSSGVGSSSGRSSVAERS 60

Query: 62  PSS-------MSWPTRKPEVPNSNG-ADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKL 121
            SS       + WP  +     S+G   K     +D+   ++  + +SEIE++KER +KL
Sbjct: 61  VSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKL 120

Query: 122 LLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSD 181
           LLGEDMSG G GVC ALAISNAITNL A + GQ WRLEP+P EKK+MWRRE++ LL VSD
Sbjct: 121 LLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSD 180

Query: 182 QIVELTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQ 241
            IVEL P++Q FP+G+K+EVM CRPRSDL+  LPALRKLDNML+EILDSF + EFWYVDQ
Sbjct: 181 HIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQ 240

Query: 242 GILAHETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMA 301
           GI+A E+  S+SFR       +KWWLP+PRVP  GL E +R++L H RE TNQILKA M+
Sbjct: 241 GIVAAESARSNSFR----EDGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMS 300

Query: 302 INNTTLADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEI 361
           IN+  LA++++P+ YLEALPKNGR+CLGD +YR I+SD FS + LL+ +++SSE    E+
Sbjct: 301 INSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEM 360

Query: 362 VNRVEASIYVWQKRINSKPATNSTKHSSKSSWEM-VKELMV---DSEKRELLAERAETLL 421
            NRVEAS+YVW++R +S+    S   S+ + W M VKE+M+   D +KRE+ AERAE+LL
Sbjct: 361 ANRVEASMYVWRRRAHSRHLI-SLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLL 420

Query: 422 LCLKQRFPGLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTK 481
           + LKQRFPGL QT LD SKIQYNKDVGKSILESYSRVLESLA+++  RI+++L++DD++K
Sbjct: 421 IRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISK 480

Query: 482 H---------SDQLSSISK------LGVISHK-NSPLHFSVPISG--TPYKT---PFATP 541
                     SD+L  +SK       G +  K ++P  FSV  SG  TPY+T     +TP
Sbjct: 481 DDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFSASTP 540

Query: 542 SFSPG--ASPAK----GERSPFFSGTKIPQR-GFGVKKVLTDYL 546
           S+SP    SP      GER+PF SG  I +R GFG KK L +YL
Sbjct: 541 SYSPMPLISPINGGRGGERAPFLSGRNIRERCGFGPKKALANYL 577

BLAST of MC01g0404 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 490.0 bits (1260), Expect = 3.9e-137
Identity = 277/527 (52.56%), Postives = 358/527 (67.93%), Query Frame = 0

Query: 31  GRESSSSSDFLTSETTGLEEHSHSGSDDSSSPS-----------SMSWPTRKPEVPNSNG 90
           G  SS   D ++SE  G    S   S+  SS S           S S P+  P V    G
Sbjct: 2   GSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPS-SPRVVAGRG 61

Query: 91  ---------ADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTAL 150
                       GGK+ V + K  +  +  SEIEMMKERF+KLLLGEDMSG G GVCTAL
Sbjct: 62  FYFPAPVMLPVIGGKDVVWDDK--QPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTAL 121

Query: 151 AISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTPTWQTFPDGSK 210
           AISNAITNL AT+FG+LWRLEPL  +KK MWRRE++WLLCVSD IVEL P+ Q FP G  
Sbjct: 122 AISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGT 181

Query: 211 LEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHETDGSSSFRGAL 270
            E+M  RPRSDLY NLPAL+KLD ML+++LD+F D EFWY D+GI+  + D  S    A 
Sbjct: 182 YEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPAS 241

Query: 271 QRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLADLDIPEPYLE 330
            RQE+KWWLP P+VPP GL E++R++LQ  R+  NQILKAA+AIN+  LA+++IP+PYLE
Sbjct: 242 VRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLE 301

Query: 331 ALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEASIYVWQKRINS 390
            LPK+G+ CLG++IY+Y+++++FSPECLLDCL++SSEHQ  EI NR+EA+++VW+++ N 
Sbjct: 302 TLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQK-NG 361

Query: 391 KPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQ 450
           +      K    S    VK L+ D+E+ + L +RAETLL  L+ RFPGLPQTTLDM+KIQ
Sbjct: 362 RRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQ 421

Query: 451 YNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKLGVISHKNSPL 510
           YNKDVG+SILESYSRV+ES+AFN+ ARIDD+LYVDD  + S  ++    L  I+  N   
Sbjct: 422 YNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQK 481

Query: 511 HFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGV 538
            FSV    +P+ +PFATP+ S  AS +     P +S  +   R  G+
Sbjct: 482 AFSV--QSSPHGSPFATPALSV-ASRSPRRAPPLYSVKRNGTREKGI 521

BLAST of MC01g0404 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 2.1e-119
Identity = 238/438 (54.34%), Postives = 311/438 (71.00%), Query Frame = 0

Query: 43  SETT-GLEEHSHSGSDDSSSPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSS 102
           SETT G  +     +   S  S  +WP       + +     G     N  L  +  S  
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNWPVL---TESKSSKCLSGLEMQSNECLVVQEISEP 106

Query: 103 EIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMW 162
           E+E MKERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEPL  EK+ +W
Sbjct: 107 ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALW 166

Query: 163 RREMDWLLCVSDQIVELTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILD 222
           +REM+ LL V D IVE  P  Q   +G+ +EVM  RPR+D+Y+NLPALRKLD+ML+E LD
Sbjct: 167 KREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALD 226

Query: 223 SFEDCEFWYVDQGILAHETDGSS--SFRGAL-QRQEEKWWLPVPRVPPGGLHEDSRRQLQ 282
           SF++ EFWY ++G L+ ++  SS  SFR  + QR+EEKWWLPVP VP  GL + +R+QL+
Sbjct: 227 SFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLK 286

Query: 283 HKRESTNQILKAAMAINNTTLADLDIPEPYLEALPKNGRACLGDLIYRYIS-SDQFSPEC 342
           +KRESTNQI KAAMAIN++ L++++IP+ Y+  LPK G++ +GD IYRY+S S +F PE 
Sbjct: 287 NKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQ 346

Query: 343 LLDCLEMSSEHQATEIVNRVEASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSE- 402
           LLDCL +SSEH+A ++ +RVEAS+Y W+++        S   +SK+SW MVK+LM  +E 
Sbjct: 347 LLDCLNISSEHEAVQLADRVEASMYTWRRK--------SCLSNSKNSWNMVKDLMSTTER 406

Query: 403 --KRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNV 462
             K  ++AERAETLL CLKQR+P L QT+LD+ KIQYNKDVGK++LESYSRVLE LAFN+
Sbjct: 407 TDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNI 466

Query: 463 VARIDDLLYVDDLTKHSD 473
           VA IDD+LYVD   + S+
Sbjct: 467 VAWIDDVLYVDKTMRGSE 473

BLAST of MC01g0404 vs. NCBI nr
Match: XP_022151538.1 (rop guanine nucleotide exchange factor 7-like [Momordica charantia])

HSP 1 Score: 1180 bits (3052), Expect = 0.0
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS 60
           MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS
Sbjct: 1   MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS 60

Query: 61  SPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMS 120
           SPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMS
Sbjct: 61  SPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMS 120

Query: 121 GCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTP 180
           GCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTP
Sbjct: 121 GCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTP 180

Query: 181 TWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET 240
           TWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET
Sbjct: 181 TWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET 240

Query: 241 DGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLA 300
           DGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLA
Sbjct: 241 DGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLA 300

Query: 301 DLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEAS 360
           DLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEAS
Sbjct: 301 DLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEAS 360

Query: 361 IYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLP 420
           IYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLP
Sbjct: 361 IYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLP 420

Query: 421 QTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKL 480
           QTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKL
Sbjct: 421 QTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKL 480

Query: 481 GVISHKNSPLHFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGVKKV 540
           GVISHKNSPLHFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGVKKV
Sbjct: 481 GVISHKNSPLHFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGVKKV 540

Query: 541 LTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           LTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE
Sbjct: 541 LTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593

BLAST of MC01g0404 vs. NCBI nr
Match: XP_038882074.1 (rop guanine nucleotide exchange factor 7-like [Benincasa hispida])

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 541/598 (90.47%), Postives = 558/598 (93.31%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           MEESV +KS+ IG IV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSSS
Sbjct: 1   MEESVIEKSQSIGDIVSENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60

Query: 62  PSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 121
           PSS+SW TRK EVP+   +NG   G KNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSLSWSTRKVEVPDCIRTNGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 122 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVEL 181
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKKVMWRREMDWLLCVSD IVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKVMWRREMDWLLCVSDHIVEL 180

Query: 182 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH 241
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH 240

Query: 242 ETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTT 301
           ETDGSSS +  LQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAIN+TT
Sbjct: 241 ETDGSSSLKRVLQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 302 LADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVE 361
           LADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 362 ASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 421
           ASIYVW+KRINSKPATNSTK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRINSKPATNSTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 422 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSSI 481
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 482 SKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRGF 541
           SKLGVISHKNSPLHF VP+SGTPYKTPFATPSFSP   ASPAKGERSPF   TKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPVSGTPYKTPFATPSFSPAQTASPAKGERSPFIPSTKIPQRGF 540

Query: 542 GVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           GVKKVLTDYLSVE RGGKEFSNK EK EP TN   ++Q ++YR G AS+P+QG VVEE
Sbjct: 541 GVKKVLTDYLSVEARGGKEFSNKIEKAEPTTNSKLDRQASQYRGGLASSPRQGSVVEE 598

BLAST of MC01g0404 vs. NCBI nr
Match: KAA0045120.1 (rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa] >TYK23617.1 rop guanine nucleotide exchange factor 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 536/599 (89.48%), Postives = 553/599 (92.32%), Query Frame = 0

Query: 1   MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS 60
           +M ES+ +KS+ IGHIV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSS
Sbjct: 59  VMAESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSS 118

Query: 61  SPSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 120
           SPSS SW TRK E P+   +NG   GGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE
Sbjct: 119 SPSSSSWSTRKAEAPDCIKANGVVNGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 178

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK+MWRREMDWLLCVSD IVE
Sbjct: 179 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVE 238

Query: 181 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 240
           LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA
Sbjct: 239 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 298

Query: 241 HETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNT 300
            ETDGSSS    LQRQEEKWWLPVPRVPPGGL EDSRRQLQHKRESTNQILKAAMAIN+T
Sbjct: 299 PETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQEDSRRQLQHKRESTNQILKAAMAINST 358

Query: 301 TLADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRV 360
           TLADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRV
Sbjct: 359 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRV 418

Query: 361 EASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 420
           EASIYVW+KR NSKPATN+TK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP
Sbjct: 419 EASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 478

Query: 421 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSS 480
           GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSS
Sbjct: 479 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSS 538

Query: 481 ISKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRG 540
           ISKLGVISHKNSPLHF VPISGTPYKTPFATPSFSP   ASPAKGERSPF  GTKIPQRG
Sbjct: 539 ISKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRG 598

Query: 541 FGVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           FGVKKVLTDYLSVE RGGKE SNK EK EP+TN   E+Q ++YR G AS+PKQ  VV E
Sbjct: 599 FGVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQTSQYRGGVASSPKQRSVVGE 657

BLAST of MC01g0404 vs. NCBI nr
Match: XP_008448594.1 (PREDICTED: rop guanine nucleotide exchange factor 7-like [Cucumis melo])

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 536/598 (89.63%), Postives = 552/598 (92.31%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           M ES+ +KS+ IGHIV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSSS
Sbjct: 1   MAESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60

Query: 62  PSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 121
           PSS SW TRK E P+   +NG   GGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 122 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVEL 181
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK+MWRREMDWLLCVSD IVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 182 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH 241
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 242 ETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTT 301
           ETDGSSS    LQRQEEKWWLPVPRVPPGGL EDSRRQLQHKRESTNQILKAAMAIN+TT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQEDSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 302 LADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVE 361
           LADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 362 ASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 421
           ASIYVW+KR NSKPATN+TK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 422 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSSI 481
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 482 SKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRGF 541
           SKLGVISHKNSPLHF VPISGTPYKTPFATPSFSP   ASPAKGERSPF  GTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 542 GVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           GVKKVLTDYLSVE RGGKE SNK EK EP+TN   E+Q ++YR G AS+PKQ  VV E
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQTSQYRGGVASSPKQRSVVGE 598

BLAST of MC01g0404 vs. NCBI nr
Match: XP_004147914.1 (rop guanine nucleotide exchange factor 7 [Cucumis sativus] >KGN54313.1 hypothetical protein Csa_017901 [Cucumis sativus])

HSP 1 Score: 1041 bits (2691), Expect = 0.0
Identity = 534/598 (89.30%), Postives = 552/598 (92.31%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           MEES+ +KS+ IGHIV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSS 
Sbjct: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSP 60

Query: 62  PSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 121
           PSS SW TRK E P+   +NG   G KNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 122 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVEL 181
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK+MWRREMDWLLCVSD IVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 182 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH 241
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 242 ETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTT 301
           ETDGSSS    LQRQEEKWWLPVPRVPPGGL E+SRRQLQHKRESTNQILKAAMAIN+TT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 302 LADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVE 361
           LADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 362 ASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 421
           ASIYVW+KR NSKPATN+TK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 422 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSSI 481
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 482 SKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRGF 541
           SKLGVISHKNSPLHF VPISGTPYKTPFATPSFSP   ASPAKGERSPF  GTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 542 GVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           GVKKVLTDYLSVE RGGKE SNK EK EP+TN   E+Q ++YR G AS+P+Q  VVEE
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 598

BLAST of MC01g0404 vs. ExPASy TrEMBL
Match: A0A6J1DCF9 (rop guanine nucleotide exchange factor 7-like OS=Momordica charantia OX=3673 GN=LOC111019452 PE=4 SV=1)

HSP 1 Score: 1180 bits (3052), Expect = 0.0
Identity = 593/593 (100.00%), Postives = 593/593 (100.00%), Query Frame = 0

Query: 1   MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS 60
           MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS
Sbjct: 1   MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS 60

Query: 61  SPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMS 120
           SPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMS
Sbjct: 61  SPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMS 120

Query: 121 GCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTP 180
           GCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTP
Sbjct: 121 GCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTP 180

Query: 181 TWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET 240
           TWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET
Sbjct: 181 TWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET 240

Query: 241 DGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLA 300
           DGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLA
Sbjct: 241 DGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLA 300

Query: 301 DLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEAS 360
           DLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEAS
Sbjct: 301 DLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEAS 360

Query: 361 IYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLP 420
           IYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLP
Sbjct: 361 IYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLP 420

Query: 421 QTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKL 480
           QTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKL
Sbjct: 421 QTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKL 480

Query: 481 GVISHKNSPLHFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGVKKV 540
           GVISHKNSPLHFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGVKKV
Sbjct: 481 GVISHKNSPLHFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGVKKV 540

Query: 541 LTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           LTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE
Sbjct: 541 LTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593

BLAST of MC01g0404 vs. ExPASy TrEMBL
Match: A0A5A7TPL2 (Rop guanine nucleotide exchange factor 7-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G001310 PE=4 SV=1)

HSP 1 Score: 1043 bits (2697), Expect = 0.0
Identity = 536/599 (89.48%), Postives = 553/599 (92.32%), Query Frame = 0

Query: 1   MMEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSS 60
           +M ES+ +KS+ IGHIV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSS
Sbjct: 59  VMAESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSS 118

Query: 61  SPSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 120
           SPSS SW TRK E P+   +NG   GGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE
Sbjct: 119 SPSSSSWSTRKAEAPDCIKANGVVNGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGE 178

Query: 121 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVE 180
           DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK+MWRREMDWLLCVSD IVE
Sbjct: 179 DMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVE 238

Query: 181 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 240
           LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA
Sbjct: 239 LTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 298

Query: 241 HETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNT 300
            ETDGSSS    LQRQEEKWWLPVPRVPPGGL EDSRRQLQHKRESTNQILKAAMAIN+T
Sbjct: 299 PETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQEDSRRQLQHKRESTNQILKAAMAINST 358

Query: 301 TLADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRV 360
           TLADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRV
Sbjct: 359 TLADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRV 418

Query: 361 EASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 420
           EASIYVW+KR NSKPATN+TK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP
Sbjct: 419 EASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFP 478

Query: 421 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSS 480
           GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSS
Sbjct: 479 GLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSS 538

Query: 481 ISKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRG 540
           ISKLGVISHKNSPLHF VPISGTPYKTPFATPSFSP   ASPAKGERSPF  GTKIPQRG
Sbjct: 539 ISKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRG 598

Query: 541 FGVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           FGVKKVLTDYLSVE RGGKE SNK EK EP+TN   E+Q ++YR G AS+PKQ  VV E
Sbjct: 599 FGVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQTSQYRGGVASSPKQRSVVGE 657

BLAST of MC01g0404 vs. ExPASy TrEMBL
Match: A0A1S3BJF9 (rop guanine nucleotide exchange factor 7-like OS=Cucumis melo OX=3656 GN=LOC103490720 PE=4 SV=1)

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 536/598 (89.63%), Postives = 552/598 (92.31%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           M ES+ +KS+ IGHIV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSSS
Sbjct: 1   MAESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSS 60

Query: 62  PSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 121
           PSS SW TRK E P+   +NG   GGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 122 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVEL 181
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK+MWRREMDWLLCVSD IVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 182 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH 241
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 242 ETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTT 301
           ETDGSSS    LQRQEEKWWLPVPRVPPGGL EDSRRQLQHKRESTNQILKAAMAIN+TT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQEDSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 302 LADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVE 361
           LADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 362 ASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 421
           ASIYVW+KR NSKPATN+TK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 422 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSSI 481
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 482 SKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRGF 541
           SKLGVISHKNSPLHF VPISGTPYKTPFATPSFSP   ASPAKGERSPF  GTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 542 GVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           GVKKVLTDYLSVE RGGKE SNK EK EP+TN   E+Q ++YR G AS+PKQ  VV E
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQTSQYRGGVASSPKQRSVVGE 598

BLAST of MC01g0404 vs. ExPASy TrEMBL
Match: A0A0A0KXQ7 (PRONE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G303120 PE=4 SV=1)

HSP 1 Score: 1041 bits (2691), Expect = 0.0
Identity = 534/598 (89.30%), Postives = 552/598 (92.31%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           MEES+ +KS+ IGHIV ENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGS+DSS 
Sbjct: 1   MEESLIEKSQTIGHIVLENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSEDSSP 60

Query: 62  PSSMSWPTRKPEVPN---SNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 121
           PSS SW TRK E P+   +NG   G KNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED
Sbjct: 61  PSSSSWSTRKAEAPDCIKANGVVNGDKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGED 120

Query: 122 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVEL 181
           MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKK+MWRREMDWLLCVSD IVEL
Sbjct: 121 MSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKLMWRREMDWLLCVSDHIVEL 180

Query: 182 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAH 241
           TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILA 
Sbjct: 181 TPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAP 240

Query: 242 ETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTT 301
           ETDGSSS    LQRQEEKWWLPVPRVPPGGL E+SRRQLQHKRESTNQILKAAMAIN+TT
Sbjct: 241 ETDGSSSLERMLQRQEEKWWLPVPRVPPGGLQENSRRQLQHKRESTNQILKAAMAINSTT 300

Query: 302 LADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVE 361
           LADL+IPEPYLEALPKNGRACLGDLIYRYISSD FSPECLLDCLEMSSEHQATEI NRVE
Sbjct: 301 LADLEIPEPYLEALPKNGRACLGDLIYRYISSDHFSPECLLDCLEMSSEHQATEIANRVE 360

Query: 362 ASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 421
           ASIYVW+KR NSKPATN+TK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG
Sbjct: 361 ASIYVWRKRTNSKPATNTTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPG 420

Query: 422 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD-QLSSI 481
           LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSD QLSSI
Sbjct: 421 LPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDHQLSSI 480

Query: 482 SKLGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRGF 541
           SKLGVISHKNSPLHF VPISGTPYKTPFATPSFSP   ASPAKGERSPF  GTKIPQRGF
Sbjct: 481 SKLGVISHKNSPLHFPVPISGTPYKTPFATPSFSPAQTASPAKGERSPFIPGTKIPQRGF 540

Query: 542 GVKKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           GVKKVLTDYLSVE RGGKE SNK EK EP+TN   E+Q ++YR G AS+P+Q  VVEE
Sbjct: 541 GVKKVLTDYLSVEARGGKELSNKIEKAEPITNSKLERQASQYRGGVASSPRQRSVVEE 598

BLAST of MC01g0404 vs. ExPASy TrEMBL
Match: A0A6J1GEC1 (rop guanine nucleotide exchange factor 7-like OS=Cucurbita moschata OX=3662 GN=LOC111453380 PE=4 SV=1)

HSP 1 Score: 1013 bits (2618), Expect = 0.0
Identity = 525/596 (88.09%), Postives = 541/596 (90.77%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEE--HSHSGSDDS 61
           MEESV+ K   IGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEE  HSHSGS++S
Sbjct: 1   MEESVSGKGLSIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEEEHSHSGSEES 60

Query: 62  SSPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDM 121
           SS S +SW TR  E P+   A     N  DNRKLEKKVSSSSEIEMMKERFSKLLLGEDM
Sbjct: 61  SSLSPLSWSTRFAEGPDCIRA-----NGADNRKLEKKVSSSSEIEMMKERFSKLLLGEDM 120

Query: 122 SGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELT 181
           SGCGNGVCTALAISNAITNLCATLFGQLWRLEPLP EKKVMWRREMDWLLCVSD IVELT
Sbjct: 121 SGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPSEKKVMWRREMDWLLCVSDHIVELT 180

Query: 182 PTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHE 241
           PTWQTFPDGSKLEVM CRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHE
Sbjct: 181 PTWQTFPDGSKLEVMACRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHE 240

Query: 242 TDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTL 301
           TDGSSS + ALQRQE KWWLPVPRVPP GL EDSRRQLQHKRESTNQILKAAMAIN+TTL
Sbjct: 241 TDGSSSLKRALQRQEGKWWLPVPRVPPDGLREDSRRQLQHKRESTNQILKAAMAINSTTL 300

Query: 302 ADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEA 361
           ADL+IPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQA EI NRVEA
Sbjct: 301 ADLEIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQAIEIANRVEA 360

Query: 362 SIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGL 421
           SIYVW+++ NSKPATNSTK SSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGL
Sbjct: 361 SIYVWRRKTNSKPATNSTKQSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGL 420

Query: 422 PQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISK 481
           PQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISK
Sbjct: 421 PQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISK 480

Query: 482 LGVISHKNSPLHFSVPISGTPYKTPFATPSFSPG--ASPAKGERSPFFSGTKIPQRGFGV 541
           LGVISHKNSPLHFSVP+SGTPYKTPFATPSFSP   ASPAKGERSP   GTKIPQRGFGV
Sbjct: 481 LGVISHKNSPLHFSVPVSGTPYKTPFATPSFSPAQSASPAKGERSPLIPGTKIPQRGFGV 540

Query: 542 KKVLTDYLSVETRGGKEFSNKTEKPEPVTNPNFEKQGARYREGSASAPKQGLVVEE 593
           KKVLTDYLSVE RGGKEFSNK EKPE  TN   + Q   YR G AS+P+QG V +E
Sbjct: 541 KKVLTDYLSVEARGGKEFSNKIEKPESFTNSKLDTQ---YRGGVASSPRQGSVADE 588

BLAST of MC01g0404 vs. TAIR 10
Match: AT5G02010.1 (RHO guanyl-nucleotide exchange factor 7 )

HSP 1 Score: 663.7 bits (1711), Expect = 1.4e-190
Identity = 361/528 (68.37%), Postives = 414/528 (78.41%), Query Frame = 0

Query: 27  IEEHGRESSSSSDFLTSETTGLEEHSHSGS---DDSSSPSSMSWPTRKPEVPNSNGADKG 86
           +EE GRESS  S    SETT  EE   S S   D ++SP S  W                
Sbjct: 11  VEEKGRESSCCS----SETTRQEEEEQSPSCTEDFTASPVSSRWSV-------------- 70

Query: 87  GKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATL 146
            KN    +K  +  S  SE+EMMKERFSKLLLGEDMSG GNGVCTALAISNAITNLCATL
Sbjct: 71  -KNIDGEKKKIRSDSRVSEVEMMKERFSKLLLGEDMSGSGNGVCTALAISNAITNLCATL 130

Query: 147 FGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTPTWQTFPDGSKLEVMTCRPRSDLY 206
           FGQLWRLEPLP EKK MWRREM+WLLCVSD IVE+TPTWQTFPDG+KLE+MTCRPRSDLY
Sbjct: 131 FGQLWRLEPLPTEKKEMWRREMEWLLCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLY 190

Query: 207 VNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHET--DGSSSFRGALQRQEEKWWLPV 266
           VNLPALRKLDNMLLEILDSFE+ EFWYVDQGI+AHE+  DGSSSFR + QRQE+KWWLPV
Sbjct: 191 VNLPALRKLDNMLLEILDSFEETEFWYVDQGIMAHESAADGSSSFRKSFQRQEDKWWLPV 250

Query: 267 PRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLADLDIPEPYLEALPKNGRACLG 326
           PRV PGGL E+SR+QLQHKR+ TNQILKAAMAIN+ TLAD++IPE YLE+LP+ GR+CLG
Sbjct: 251 PRVSPGGLQENSRKQLQHKRDCTNQILKAAMAINSITLADMEIPESYLESLPRKGRSCLG 310

Query: 327 DLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEASIYVWQKRINSKPATNSTKHSS 386
           DLIYRYISSDQFSPECLLDCL++SSEHQA EI NRVE+SIY+W KR NSKPATN     +
Sbjct: 311 DLIYRYISSDQFSPECLLDCLDLSSEHQAIEIANRVESSIYLWHKRTNSKPATN-----T 370

Query: 387 KSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKSILE 446
           K+SWEMVKELMVD++K EL+A+RAE+LLL LKQRFPGLPQT LDMSKIQYNKD+GKSILE
Sbjct: 371 KTSWEMVKELMVDADKLELMADRAESLLLSLKQRFPGLPQTALDMSKIQYNKDIGKSILE 430

Query: 447 SYSRVLESLAFNVVARIDDLLYVDDLTKH-SDQLSSISKLGVISHKNSPLHFSVPISGTP 506
           SYSRVLESLAFN+VARIDDLL+VDDLT+H SDQ+   + LG  +  ++P   +VP+S   
Sbjct: 431 SYSRVLESLAFNIVARIDDLLFVDDLTRHSSDQIP--TTLG-NNGNDAPKSIAVPVSN-- 490

Query: 507 YKTPFATPSFSPGASPAKGERS--PFFSGTKIPQRGFGVKKVLTDYLS 547
               + TPS+SP     +   +  P  S  KIP     VK+VLT Y++
Sbjct: 491 ----YTTPSYSPSKQELRSSITVPPSPSRFKIPHSS-SVKRVLTAYVT 504

BLAST of MC01g0404 vs. TAIR 10
Match: AT5G05940.1 (ROP guanine nucleotide exchange factor 5 )

HSP 1 Score: 608.6 bits (1568), Expect = 5.3e-174
Identity = 327/557 (58.71%), Postives = 406/557 (72.89%), Query Frame = 0

Query: 15  HIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSSPSSMSWPTRKPEV 74
           ++ P  E +  L E   R S+S + + +S TT +     S S        + WP RK   
Sbjct: 17  NLTPSMEDI--LTESKERSSTSGASYESSSTTTVA----SSSPPPPPSQILGWPIRKASF 76

Query: 75  PNSNGADKGGKNHV-----DNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTA 134
              N  +    +H      D+   + K  +S+++EMMKERF+KLLLGEDMSG G GVCTA
Sbjct: 77  -RKNSKESVNFDHKKLTLHDDSGFKAKEMNSADVEMMKERFAKLLLGEDMSGSGKGVCTA 136

Query: 135 LAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTPTWQTFPDGS 194
           LAISNAITNLCAT+FGQLWRLEPL  EKK MWRREM+W+L VSD IVELTP+ QT+PDG+
Sbjct: 137 LAISNAITNLCATIFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGN 196

Query: 195 KLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHETDGSSSFRGA 254
           K EVMTCRPR DL++NLPALRKLDNMLL+IL SF+  EFWYVDQGI+A E DGS+SFR  
Sbjct: 197 KFEVMTCRPRFDLFINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDGSASFRRK 256

Query: 255 LQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLADLDIPEPYL 314
           +QRQEEKWWLPVPR+ P GL E++R +L HKRE   QILKAAMAIN+  L ++D+P+ YL
Sbjct: 257 IQRQEEKWWLPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYL 316

Query: 315 EALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEASIYVWQKRIN 374
           E LPKNGR+CLGD+IYRY++SD+FS E LLDCL++SSEH A +I NRVEASIYVW++R+ 
Sbjct: 317 ETLPKNGRSCLGDVIYRYVTSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQ 376

Query: 375 SKPATNSTKHSS--KSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMS 434
           +K   N+   S+  K +WEMVKELM   +KR LL ER+ETLL CLKQRFP L QT+LD+S
Sbjct: 377 TKLGVNNNTSSTTPKLTWEMVKELMAAGDKRGLLVERSETLLRCLKQRFPSLTQTSLDIS 436

Query: 435 KIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQ---LSSISKLGVIS 494
           KIQ+NKD+GKSILESYSR LESLA N++ARIDDLLYVDDLTK SD    LSS +   +I+
Sbjct: 437 KIQWNKDIGKSILESYSRALESLASNIIARIDDLLYVDDLTKQSDDNNLLSSPAVSSIIA 496

Query: 495 HKN-SPLHFSVPISGTPYKTPFA-TPSFSPG-ASPAKGERSPFFSGTK--------IPQR 551
           HK   PL + +  SGTPY+T F+ TP FSP   SP KGER   +S           +P R
Sbjct: 497 HKKVVPLPYLISASGTPYRTSFSTTPGFSPSMISPKKGERRTPYSSKDTNKIIEKGLPSR 556

BLAST of MC01g0404 vs. TAIR 10
Match: AT3G55660.1 (ROP (rho of plants) guanine nucleotide exchange factor 6 )

HSP 1 Score: 496.9 bits (1278), Expect = 2.3e-140
Identity = 299/584 (51.20%), Postives = 394/584 (67.47%), Query Frame = 0

Query: 2   MEESVADKSEGIGHIVPENETLGHLIEEHGRESSSSSDFLTSETTGLEEHSHSGSDDSSS 61
           ME++     EG       ++ +  LI+     ++ SS  L+S ++G+   S   S    S
Sbjct: 1   MEDNSCIGFEGSKRFGETSKRIIGLIDSVTESTTDSS--LSSSSSGVGSSSGRSSVAERS 60

Query: 62  PSS-------MSWPTRKPEVPNSNG-ADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKL 121
            SS       + WP  +     S+G   K     +D+   ++  + +SEIE++KER +KL
Sbjct: 61  VSSPPTKSQILGWPLGQGSWRKSSGKMKKKTPTKIDDFGFKRVGTETSEIELLKERMAKL 120

Query: 122 LLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSD 181
           LLGEDMSG G GVC ALAISNAITNL A + GQ WRLEP+P EKK+MWRRE++ LL VSD
Sbjct: 121 LLGEDMSGSGEGVCPALAISNAITNLYAAILGQQWRLEPIPSEKKLMWRREIEVLLSVSD 180

Query: 182 QIVELTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQ 241
            IVEL P++Q FP+G+K+EVM CRPRSDL+  LPALRKLDNML+EILDSF + EFWYVDQ
Sbjct: 181 HIVELVPSFQNFPNGNKIEVMNCRPRSDLFTCLPALRKLDNMLIEILDSFGETEFWYVDQ 240

Query: 242 GILAHETDGSSSFRGALQRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMA 301
           GI+A E+  S+SFR       +KWWLP+PRVP  GL E +R++L H RE TNQILKA M+
Sbjct: 241 GIVAAESARSNSFR----EDGDKWWLPLPRVPSDGLTEQTRKKLDHTREFTNQILKACMS 300

Query: 302 INNTTLADLDIPEPYLEALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEI 361
           IN+  LA++++P+ YLEALPKNGR+CLGD +YR I+SD FS + LL+ +++SSE    E+
Sbjct: 301 INSIALAEMEVPQSYLEALPKNGRSCLGDFLYRNITSDNFSADHLLESIDLSSELAVVEM 360

Query: 362 VNRVEASIYVWQKRINSKPATNSTKHSSKSSWEM-VKELMV---DSEKRELLAERAETLL 421
            NRVEAS+YVW++R +S+    S   S+ + W M VKE+M+   D +KRE+ AERAE+LL
Sbjct: 361 ANRVEASMYVWRRRAHSRHLI-SLYRSTSTRWGMIVKEMMMHQTDGDKREIFAERAESLL 420

Query: 422 LCLKQRFPGLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTK 481
           + LKQRFPGL QT LD SKIQYNKDVGKSILESYSRVLESLA+++  RI+++L++DD++K
Sbjct: 421 IRLKQRFPGLRQTALDTSKIQYNKDVGKSILESYSRVLESLAYSIGVRIEEVLFMDDISK 480

Query: 482 H---------SDQLSSISK------LGVISHK-NSPLHFSVPISG--TPYKT---PFATP 541
                     SD+L  +SK       G +  K ++P  FSV  SG  TPY+T     +TP
Sbjct: 481 DDGDGDDDSCSDKLRLLSKEAASGGSGSLREKLSAPSLFSVSFSGTSTPYRTLSFSASTP 540

Query: 542 SFSPG--ASPAK----GERSPFFSGTKIPQR-GFGVKKVLTDYL 546
           S+SP    SP      GER+PF SG  I +R GFG KK L +YL
Sbjct: 541 SYSPMPLISPINGGRGGERAPFLSGRNIRERCGFGPKKALANYL 577

BLAST of MC01g0404 vs. TAIR 10
Match: AT4G38430.1 (rho guanyl-nucleotide exchange factor 1 )

HSP 1 Score: 490.0 bits (1260), Expect = 2.8e-138
Identity = 277/527 (52.56%), Postives = 358/527 (67.93%), Query Frame = 0

Query: 31  GRESSSSSDFLTSETTGLEEHSHSGSDDSSSPS-----------SMSWPTRKPEVPNSNG 90
           G  SS   D ++SE  G    S   S+  SS S           S S P+  P V    G
Sbjct: 2   GSLSSEEDDEVSSERCGSYSPSADISESESSSSFSCHRFDGEGASSSIPS-SPRVVAGRG 61

Query: 91  ---------ADKGGKNHVDNRKLEKKVSSSSEIEMMKERFSKLLLGEDMSGCGNGVCTAL 150
                       GGK+ V + K  +  +  SEIEMMKERF+KLLLGEDMSG G GVCTAL
Sbjct: 62  FYFPAPVMLPVIGGKDVVWDDK--QPDNDLSEIEMMKERFAKLLLGEDMSGGGKGVCTAL 121

Query: 151 AISNAITNLCATLFGQLWRLEPLPREKKVMWRREMDWLLCVSDQIVELTPTWQTFPDGSK 210
           AISNAITNL AT+FG+LWRLEPL  +KK MWRRE++WLLCVSD IVEL P+ Q FP G  
Sbjct: 122 AISNAITNLSATVFGELWRLEPLAPQKKAMWRRELEWLLCVSDSIVELIPSIQQFPGGGT 181

Query: 211 LEVMTCRPRSDLYVNLPALRKLDNMLLEILDSFEDCEFWYVDQGILAHETDGSSSFRGAL 270
            E+M  RPRSDLY NLPAL+KLD ML+++LD+F D EFWY D+GI+  + D  S    A 
Sbjct: 182 YEIMETRPRSDLYANLPALKKLDAMLIDMLDAFSDTEFWYTDRGIVLGDCDKDSYNSPAS 241

Query: 271 QRQEEKWWLPVPRVPPGGLHEDSRRQLQHKRESTNQILKAAMAINNTTLADLDIPEPYLE 330
            RQE+KWWLP P+VPP GL E++R++LQ  R+  NQILKAA+AIN+  LA+++IP+PYLE
Sbjct: 242 VRQEDKWWLPCPKVPPNGLSEEARKKLQQCRDFANQILKAALAINSGVLAEMEIPDPYLE 301

Query: 331 ALPKNGRACLGDLIYRYISSDQFSPECLLDCLEMSSEHQATEIVNRVEASIYVWQKRINS 390
            LPK+G+ CLG++IY+Y+++++FSPECLLDCL++SSEHQ  EI NR+EA+++VW+++ N 
Sbjct: 302 TLPKSGKECLGEIIYQYLTANKFSPECLLDCLDLSSEHQTLEIANRIEAAVHVWRQK-NG 361

Query: 391 KPATNSTKHSSKSSWEMVKELMVDSEKRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQ 450
           +      K    S    VK L+ D+E+ + L +RAETLL  L+ RFPGLPQTTLDM+KIQ
Sbjct: 362 RRHKKQAKLKLSSWGGKVKGLVNDNERNDFLVQRAETLLQSLRIRFPGLPQTTLDMNKIQ 421

Query: 451 YNKDVGKSILESYSRVLESLAFNVVARIDDLLYVDDLTKHSDQLSSISKLGVISHKNSPL 510
           YNKDVG+SILESYSRV+ES+AFN+ ARIDD+LYVDD  + S  ++    L  I+  N   
Sbjct: 422 YNKDVGQSILESYSRVMESMAFNITARIDDVLYVDDAMRRSISVTESLSLFSINGLNPQK 481

Query: 511 HFSVPISGTPYKTPFATPSFSPGASPAKGERSPFFSGTKIPQRGFGV 538
            FSV    +P+ +PFATP+ S  AS +     P +S  +   R  G+
Sbjct: 482 AFSV--QSSPHGSPFATPALSV-ASRSPRRAPPLYSVKRNGTREKGI 521

BLAST of MC01g0404 vs. TAIR 10
Match: AT4G00460.2 (RHO guanyl-nucleotide exchange factor 3 )

HSP 1 Score: 431.0 bits (1107), Expect = 1.5e-120
Identity = 238/438 (54.34%), Postives = 311/438 (71.00%), Query Frame = 0

Query: 43  SETT-GLEEHSHSGSDDSSSPSSMSWPTRKPEVPNSNGADKGGKNHVDNRKLEKKVSSSS 102
           SETT G  +     +   S  S  +WP       + +     G     N  L  +  S  
Sbjct: 47  SETTSGFSDQIDETNSFCSEASPCNWPVL---TESKSSKCLSGLEMQSNECLVVQEISEP 106

Query: 103 EIEMMKERFSKLLLGEDMSGCGNGVCTALAISNAITNLCATLFGQLWRLEPLPREKKVMW 162
           E+E MKERF+KLLLGEDMSG G GVCTA+ ISNAITNL AT+FGQ  RLEPL  EK+ +W
Sbjct: 107 ELETMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLETEKRALW 166

Query: 163 RREMDWLLCVSDQIVELTPTWQTFPDGSKLEVMTCRPRSDLYVNLPALRKLDNMLLEILD 222
           +REM+ LL V D IVE  P  Q   +G+ +EVM  RPR+D+Y+NLPALRKLD+ML+E LD
Sbjct: 167 KREMNCLLSVCDYIVEFIPRCQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALD 226

Query: 223 SFEDCEFWYVDQGILAHETDGSS--SFRGAL-QRQEEKWWLPVPRVPPGGLHEDSRRQLQ 282
           SF++ EFWY ++G L+ ++  SS  SFR  + QR+EEKWWLPVP VP  GL + +R+QL+
Sbjct: 227 SFQNTEFWYAEEGSLSMKSARSSTGSFRKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLK 286

Query: 283 HKRESTNQILKAAMAINNTTLADLDIPEPYLEALPKNGRACLGDLIYRYIS-SDQFSPEC 342
           +KRESTNQI KAAMAIN++ L++++IP+ Y+  LPK G++ +GD IYRY+S S +F PE 
Sbjct: 287 NKRESTNQIHKAAMAINSSILSEMEIPDSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQ 346

Query: 343 LLDCLEMSSEHQATEIVNRVEASIYVWQKRINSKPATNSTKHSSKSSWEMVKELMVDSE- 402
           LLDCL +SSEH+A ++ +RVEAS+Y W+++        S   +SK+SW MVK+LM  +E 
Sbjct: 347 LLDCLNISSEHEAVQLADRVEASMYTWRRK--------SCLSNSKNSWNMVKDLMSTTER 406

Query: 403 --KRELLAERAETLLLCLKQRFPGLPQTTLDMSKIQYNKDVGKSILESYSRVLESLAFNV 462
             K  ++AERAETLL CLKQR+P L QT+LD+ KIQYNKDVGK++LESYSRVLE LAFN+
Sbjct: 407 TDKNYVMAERAETLLFCLKQRYPELSQTSLDICKIQYNKDVGKAVLESYSRVLEGLAFNI 466

Query: 463 VARIDDLLYVDDLTKHSD 473
           VA IDD+LYVD   + S+
Sbjct: 467 VAWIDDVLYVDKTMRGSE 473