MC01g0373 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g0373 vs. NCBI nr
Match: KGN54361.1 (hypothetical protein Csa_017994 [Cucumis sativus])

HSP 1 Score: 117 bits (292), Expect = 5.02e-31
Identity = 78/122 (63.93%), Postives = 88/122 (72.13%), Query Frame = 0

Query: 1   SSSRRVSFSSGSDQLPAVPRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRLRANVAKV 60
           SS RRVSFSS  DQ  A     ++  I VFW FR+     +ARFSPE FLRRL A +AKV
Sbjct: 6   SSGRRVSFSS--DQAAAAKSRIRKRPIIVFWVFRL---PKSARFSPEKFLRRLGAKMAKV 65

Query: 61  LRYVSFKKRSCSKKNSCA---NNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFFNSSSS 119
           LRYVS +KRS S  NS +   NN +S FNRSHSVSD SME+SHRAEAVKDCI FFNSS+S
Sbjct: 66  LRYVSLRKRSTSSTNSSSLKNNNGSSKFNRSHSVSD-SMEESHRAEAVKDCIQFFNSSNS 121

BLAST of MC01g0373 vs. NCBI nr
Match: XP_022950374.1 (josephin-like protein [Cucurbita moschata])

HSP 1 Score: 115 bits (287), Expect = 3.33e-30
Identity = 78/126 (61.90%), Postives = 88/126 (69.84%), Query Frame = 0

Query: 1   SSSRRVSFSSGSDQLPAV--------PRNKKRSVITVFWAFRVIPAKSAARFSPESFLRR 60
           SSSRRVSFSS      A         PRN+KR +ITVFW FR+     + R  P SFLRR
Sbjct: 6   SSSRRVSFSSDQGAAAATSAAKPAAFPRNRKR-LITVFWVFRL---PKSGRLFPGSFLRR 65

Query: 61  LRANVAKVLRYVSFKKRSCSKKNSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFF 118
           + A V KVLRYVS +KRS S  +S +  S SNFNRSHSVS+ SME+SHRAEAVKDCINFF
Sbjct: 66  IGAKVVKVLRYVSLRKRSASPASSSSLKSGSNFNRSHSVSE-SMEESHRAEAVKDCINFF 125

BLAST of MC01g0373 vs. NCBI nr
Match: XP_023518083.1 (uncharacterized protein LOC111781626 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 112 bits (281), Expect = 2.03e-29
Identity = 79/120 (65.83%), Postives = 90/120 (75.00%), Query Frame = 0

Query: 2   SSRRVSFSS--GSDQLPAVPRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRLRANVAK 61
           SSR VS  S  G+ +   + RN+KR VITV W FR+     + RFSPESFLRR+ A VAK
Sbjct: 6   SSRGVSLRSDEGTAKPTTLQRNRKR-VITVLWVFRL---PKSGRFSPESFLRRVGAKVAK 65

Query: 62  VLRYVSFKKRS-CSKKNSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFFNSSSSS 118
           V+RYVS KKRS CSKK S A    +NFNRSHSVSD SME+SHRAEAVKDCI+FFNSSSSS
Sbjct: 66  VVRYVSLKKRSECSKKASAA----TNFNRSHSVSD-SMEESHRAEAVKDCIHFFNSSSSS 116

BLAST of MC01g0373 vs. NCBI nr
Match: KAG6595400.1 (hypothetical protein SDJN03_11953, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027410.1 hypothetical protein SDJN02_11422, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 112 bits (279), Expect = 4.20e-29
Identity = 80/121 (66.12%), Postives = 91/121 (75.21%), Query Frame = 0

Query: 2   SSRRVSFSS--GSDQLPAVPRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRLRANVAK 61
           SSR VS  S  G+ +  A+ RN+KR VITV W FR+     + RFSPESFLRR+ A VAK
Sbjct: 6   SSRGVSLRSDEGTAKPTALQRNRKR-VITVLWVFRL---PKSGRFSPESFLRRVGAKVAK 65

Query: 62  VLRYVSFKKRS-CSKKNSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFFNSSSSS 119
           VLR VS KKRS CSKK S A    +NFNRSHSVSD SME+SHRAEAV+DCI+FFNSSSSS
Sbjct: 66  VLRAVSLKKRSECSKKASAA----TNFNRSHSVSD-SMEESHRAEAVRDCIHFFNSSSSS 117

BLAST of MC01g0373 vs. NCBI nr
Match: KAG6603596.1 (hypothetical protein SDJN03_04205, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 110 bits (275), Expect = 2.54e-28
Identity = 81/131 (61.83%), Postives = 92/131 (70.23%), Query Frame = 0

Query: 1   SSSRRVSFSS------GSDQLPAV-PRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRL 60
           SSSRRVSFSS       S   PA  PRN+KR +ITVFW FR+     + R  P SFLRR+
Sbjct: 6   SSSRRVSFSSDQGAAAASAAKPAAFPRNRKR-LITVFWVFRL---PKSGRLFPGSFLRRI 65

Query: 61  RANVAKVLRYVSFKKRS-----CSKKNSCAN-NSNSNFNRSHSVSDHSMEDSHRAEAVKD 118
            A V KVLRYVS +KRS     CS  +S ++  S SNFNRSHSVS+ SME+SHRAEAVKD
Sbjct: 66  GAKVVKVLRYVSLRKRSASPASCSSSSSSSSLKSGSNFNRSHSVSE-SMEESHRAEAVKD 125

BLAST of MC01g0373 vs. ExPASy TrEMBL
Match: A0A0A0L2K7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G308510 PE=4 SV=1)

HSP 1 Score: 117 bits (292), Expect = 2.43e-31
Identity = 78/122 (63.93%), Postives = 88/122 (72.13%), Query Frame = 0

Query: 1   SSSRRVSFSSGSDQLPAVPRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRLRANVAKV 60
           SS RRVSFSS  DQ  A     ++  I VFW FR+     +ARFSPE FLRRL A +AKV
Sbjct: 6   SSGRRVSFSS--DQAAAAKSRIRKRPIIVFWVFRL---PKSARFSPEKFLRRLGAKMAKV 65

Query: 61  LRYVSFKKRSCSKKNSCA---NNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFFNSSSS 119
           LRYVS +KRS S  NS +   NN +S FNRSHSVSD SME+SHRAEAVKDCI FFNSS+S
Sbjct: 66  LRYVSLRKRSTSSTNSSSLKNNNGSSKFNRSHSVSD-SMEESHRAEAVKDCIQFFNSSNS 121

BLAST of MC01g0373 vs. ExPASy TrEMBL
Match: A0A6J1GFL9 (josephin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111453490 PE=4 SV=1)

HSP 1 Score: 115 bits (287), Expect = 1.61e-30
Identity = 78/126 (61.90%), Postives = 88/126 (69.84%), Query Frame = 0

Query: 1   SSSRRVSFSSGSDQLPAV--------PRNKKRSVITVFWAFRVIPAKSAARFSPESFLRR 60
           SSSRRVSFSS      A         PRN+KR +ITVFW FR+     + R  P SFLRR
Sbjct: 6   SSSRRVSFSSDQGAAAATSAAKPAAFPRNRKR-LITVFWVFRL---PKSGRLFPGSFLRR 65

Query: 61  LRANVAKVLRYVSFKKRSCSKKNSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFF 118
           + A V KVLRYVS +KRS S  +S +  S SNFNRSHSVS+ SME+SHRAEAVKDCINFF
Sbjct: 66  IGAKVVKVLRYVSLRKRSASPASSSSLKSGSNFNRSHSVSE-SMEESHRAEAVKDCINFF 125

BLAST of MC01g0373 vs. ExPASy TrEMBL
Match: A0A6J1IJN3 (uncharacterized protein LOC111477703 OS=Cucurbita maxima OX=3661 GN=LOC111477703 PE=4 SV=1)

HSP 1 Score: 108 bits (271), Expect = 4.31e-28
Identity = 77/127 (60.63%), Postives = 90/127 (70.87%), Query Frame = 0

Query: 1   SSSRRVSFSSGSDQLPAV------PRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRLR 60
           SSSRRVSFSS      A       PRN+KR +ITVFW F++     + R  P SFLRR+ 
Sbjct: 6   SSSRRVSFSSDQGAAAAAAKPAAFPRNRKR-LITVFWVFQL---PKSGRLFPGSFLRRIG 65

Query: 61  ANVAKVLRYVSFKKRSCSKKNSCANNSN----SNFNRSHSVSDHSMEDSHRAEAVKDCIN 117
           A V KVLRYVS +KRS S   SC+++S+    SNFNRSHSVS+ SME+SHRAEAVKDCIN
Sbjct: 66  AKVVKVLRYVSLRKRSASPA-SCSSSSSLKSGSNFNRSHSVSE-SMEESHRAEAVKDCIN 125

BLAST of MC01g0373 vs. ExPASy TrEMBL
Match: A0A6J1AQZ4 (josephin-like protein OS=Herrania umbratica OX=108875 GN=LOC110420321 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 8.73e-13
Identity = 53/125 (42.40%), Postives = 75/125 (60.00%), Query Frame = 0

Query: 3   SRRVSFSSGSDQLPAV----------PRNKKRSVITVFWAFRVIPAKSAARFSPESFLRR 62
           S+RVSFS   ++ P +           R K+R V+   + FR++    ++RFSP   LRR
Sbjct: 6   SKRVSFSPDVNERPTILLKHGGSTCRTRGKRRRVVAGIFTFRLV---RSSRFSPARLLRR 65

Query: 63  LRANVAKVLRYVSFKKRSCSKKNSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFF 117
           L A VA+ LR+VS  + S S K    ++S+SN  RS S+++    DSHRAEA++DCI F 
Sbjct: 66  LGAKVARALRFVSMGRNSNSHK---VSSSSSNLARSRSLAE--SIDSHRAEAIEDCIEFL 122

BLAST of MC01g0373 vs. ExPASy TrEMBL
Match: A0A061EQH2 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_021741 PE=4 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 1.23e-12
Identity = 52/125 (41.60%), Postives = 75/125 (60.00%), Query Frame = 0

Query: 3   SRRVSFSSGSDQLPAV----------PRNKKRSVITVFWAFRVIPAKSAARFSPESFLRR 62
           S+RVSFS   ++ P +           R  +R V+   + FR++    ++RFSP   LRR
Sbjct: 6   SKRVSFSPDVNERPTILLKHGGSTGRTRGNRRRVVAGIFTFRLV---RSSRFSPARLLRR 65

Query: 63  LRANVAKVLRYVSFKKRSCSKKNSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFF 117
           L A VA+ LR+VS ++ S S K    ++S+SN  RS S+++    DSHRAEA++DCI F 
Sbjct: 66  LGAKVARALRFVSMRRNSNSHK---VSSSSSNLARSRSLAE--SIDSHRAEAIEDCIEFL 122

BLAST of MC01g0373 vs. TAIR 10
Match: AT3G09032.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G01225.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 40.8 bits (94), Expect = 8.9e-04
Identity = 33/113 (29.20%), Postives = 62/113 (54.87%), Query Frame = 0

Query: 13  DQLPAVPRNKKRSVITVFWAFRVIPAKSAARFSPESFLRRLRANVAKVLRYVSFKKRSCS 72
           D L +   +++R V+    +F +  + +A +      ++R+ A  AK LR++SF++ +  
Sbjct: 27  DGLSSSHHSRRRVVLVGILSFGLRSSPAARK-----LIQRIGARFAKTLRFISFRRNTTD 86

Query: 73  KK----------NSCANNSNSNFNRSHSVSDHSMEDSHRAEAVKDCINFFNSS 116
           ++          +S +++S     RS SV++    +SHRAEA++DCI F NSS
Sbjct: 87  RRKTSSFLLPSSSSSSSSSAIYMKRSKSVNE---TESHRAEAIEDCIEFLNSS 131