MC01g0330 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0330
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RING-type E3 ubiquitin transferase
Location	MC01: 9979146 .. 9980903 (-)
RNA-Seq Expression	MC01g0330
Synteny	MC01g0330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCATTTTTCTCTCCCATGGCAGAAGATAATCAAGTTGAAGTTGATACTCCTCCTTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAAGATCCGGTGACGGTGGCGTCCGGCATAACCTACGACCGGGAGAGCATTGAGAAGTGGCTGTTTTCCGACAAACATACCACGTGCCCCGTCACACAGGTGGTCTTGTCGAACTCCGATCTCACTCCCAACCATACTCTTCGACGAGTGATTCAGGCTTGGTGCACTCTCAATGCTCGTAGCGGCAGCGTCGAGAGGTTTCCGACACCGAAGCCTCCTGTCGACCGGACGCAGGTCGTCAAGATTCTGGCCGATGTGAATTTGTCTCCGTTCTCCCAACAGAAATGTCTCCAACGACTTCGATCCATTGCATCGGCGAATGAAACAAACAAACGCTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCCGGAGTTGTGTGTAATTCCATCAACGTGGAATCCAGCTACCAAGGTAAATCCCAGAATCATTTTAAACCTTAATTATTTTTTTTTTCTAAAATAATGAGAAAAATCTAAAAAATTGTCAATTTTTTTTATCGTAGTAAACACATCGGCATTATTCATATTTAAGAGGTTATTTGTTTAAAGTCTATTTTTTTAATACAATATGTAGAAATAATGGATCGAATCCACGTCCTTTTGATTAGAGATATATATCAAATATGTGGGGTGGAGAATCAAACTCAGTTTATTGATTTGAGATATATACCAAACATGTGGGGTGAAGAATCGAACTCAGTCTATTAATTTGAGATATATATTAATTTGTACTCGGTTTTTAAAACCTCTTTAGATTGGTCTTACGACGAAGAAAAAAAAAATATATATCCATAAATTTTGAGATGTTAAATTTGCCCCTCTATTGCGAAAATTTCCAACTATGACCTTCAACCATCATAAAAATAGAGCTAATTTTATTATGATTATTATTTTTTAAATACAGATTATGCGTTTGATGACGAGTTAGCAAATTTGGGCGACGAGGCTTTGAGCATTCTTCACAAACTCCAAATCTCTGAAGCCGGACTGAAATTTCTTCTCAGCAACAATGGCGGCGTCTTCGTCGCGTCCTTAATCAAAGTCCTGCAGAACGGAACCTACGGATCTCGCGCTTACGCCGTAATGCTATTGAAATCGATCTTCGAAGTCGCCGATCGGATCCAGATCGTTAGCGTAACCGCCGAATTCTTCATCGAAATCGTTCAAGTTCTGAAGGATCAGTTCTCGCAACAGGCCACGAAAGCGGCATTGAAACTTCTAATCACTCTCTGTTCGAGTTTCAGAAACAGAGTAAAGGCGATTCAGGCCGGCGCGGTTCCGGTTCTGATCGATCTCCTCCTCGACCTCGATTCGTCGGAGAAGCGAGCGTGCGAGATGATTCTGATGGCACTGGATCTGCTCTGCGGGTGCGCCGACGGCCGGGCGGAGCTGCTTAACCATGCGGCGGGGCTCGCCGTCGTATCGAAGAAGATTCTGAGAGTTTCTTGGCTCGGAAGCGAGAAGGCCGTCGGAATCTTGTGCTCCGTCGCGAAGTTCTCCGCGAGCCAAAGCGTCGTCCAGGAGATGCTGCGGCTCGGAGTTGTGACGAAGCTGTGCTTTCTACTGCAAGTCGTCGGCGCCGGAAGTAAAGCGAAGGAGAAAGCCAAAGAGATTCTGAAGCTCCATGGTCGGACATGGCGGAATTCTTCTTGCTTGCCTTTGAGTTTG

mRNA sequence

TCATTTTTCTCTCCCATGGCAGAAGATAATCAAGTTGAAGTTGATACTCCTCCTTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAAGATCCGGTGACGGTGGCGTCCGGCATAACCTACGACCGGGAGAGCATTGAGAAGTGGCTGTTTTCCGACAAACATACCACGTGCCCCGTCACACAGGTGGTCTTGTCGAACTCCGATCTCACTCCCAACCATACTCTTCGACGAGTGATTCAGGCTTGGTGCACTCTCAATGCTCGTAGCGGCAGCGTCGAGAGGTTTCCGACACCGAAGCCTCCTGTCGACCGGACGCAGGTCGTCAAGATTCTGGCCGATGTGAATTTGTCTCCGTTCTCCCAACAGAAATGTCTCCAACGACTTCGATCCATTGCATCGGCGAATGAAACAAACAAACGCTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCCGGAGTTGTGTGTAATTCCATCAACGTGGAATCCAGCTACCAAGCAAATTTGGGCGACGAGGCTTTGAGCATTCTTCACAAACTCCAAATCTCTGAAGCCGGACTGAAATTTCTTCTCAGCAACAATGGCGGCGTCTTCGTCGCGTCCTTAATCAAAGTCCTGCAGAACGGAACCTACGGATCTCGCGCTTACGCCGTAATGCTATTGAAATCGATCTTCGAAGTCGCCGATCGGATCCAGATCGTTAGCGTAACCGCCGAATTCTTCATCGAAATCGTTCAAGTTCTGAAGGATCAGTTCTCGCAACAGGCCACGAAAGCGGCATTGAAACTTCTAATCACTCTCTGTTCGAGTTTCAGAAACAGAGTAAAGGCGATTCAGGCCGGCGCGGTTCCGGTTCTGATCGATCTCCTCCTCGACCTCGATTCGTCGGAGAAGCGAGCGTGCGAGATGATTCTGATGGCACTGGATCTGCTCTGCGGGTGCGCCGACGGCCGGGCGGAGCTGCTTAACCATGCGGCGGGGCTCGCCGTCGTATCGAAGAAGATTCTGAGAGTTTCTTGGCTCGGAAGCGAGAAGGCCGTCGGAATCTTGTGCTCCGTCGCGAAGTTCTCCGCGAGCCAAAGCGTCGTCCAGGAGATGCTGCGGCTCGGAGTTGTGACGAAGCTGTGCTTTCTACTGCAAGTCGTCGGCGCCGGAAGTAAAGCGAAGGAGAAAGCCAAAGAGATTCTGAAGCTCCATGGTCGGACATGGCGGAATTCTTCTTGCTTGCCTTTGAGTTTG

Coding sequence (CDS)

TCATTTTTCTCTCCCATGGCAGAAGATAATCAAGTTGAAGTTGATACTCCTCCTTTCTTTCTCTGTCCCATCTCTCTGCAAATCATGAAAGATCCGGTGACGGTGGCGTCCGGCATAACCTACGACCGGGAGAGCATTGAGAAGTGGCTGTTTTCCGACAAACATACCACGTGCCCCGTCACACAGGTGGTCTTGTCGAACTCCGATCTCACTCCCAACCATACTCTTCGACGAGTGATTCAGGCTTGGTGCACTCTCAATGCTCGTAGCGGCAGCGTCGAGAGGTTTCCGACACCGAAGCCTCCTGTCGACCGGACGCAGGTCGTCAAGATTCTGGCCGATGTGAATTTGTCTCCGTTCTCCCAACAGAAATGTCTCCAACGACTTCGATCCATTGCATCGGCGAATGAAACAAACAAACGCTGCATGGAATCTGCTGGAGTTGTGGAGTTCTTGGCCGGAGTTGTGTGTAATTCCATCAACGTGGAATCCAGCTACCAAGCAAATTTGGGCGACGAGGCTTTGAGCATTCTTCACAAACTCCAAATCTCTGAAGCCGGACTGAAATTTCTTCTCAGCAACAATGGCGGCGTCTTCGTCGCGTCCTTAATCAAAGTCCTGCAGAACGGAACCTACGGATCTCGCGCTTACGCCGTAATGCTATTGAAATCGATCTTCGAAGTCGCCGATCGGATCCAGATCGTTAGCGTAACCGCCGAATTCTTCATCGAAATCGTTCAAGTTCTGAAGGATCAGTTCTCGCAACAGGCCACGAAAGCGGCATTGAAACTTCTAATCACTCTCTGTTCGAGTTTCAGAAACAGAGTAAAGGCGATTCAGGCCGGCGCGGTTCCGGTTCTGATCGATCTCCTCCTCGACCTCGATTCGTCGGAGAAGCGAGCGTGCGAGATGATTCTGATGGCACTGGATCTGCTCTGCGGGTGCGCCGACGGCCGGGCGGAGCTGCTTAACCATGCGGCGGGGCTCGCCGTCGTATCGAAGAAGATTCTGAGAGTTTCTTGGCTCGGAAGCGAGAAGGCCGTCGGAATCTTGTGCTCCGTCGCGAAGTTCTCCGCGAGCCAAAGCGTCGTCCAGGAGATGCTGCGGCTCGGAGTTGTGACGAAGCTGTGCTTTCTACTGCAAGTCGTCGGCGCCGGAAGTAAAGCGAAGGAGAAAGCCAAAGAGATTCTGAAGCTCCATGGTCGGACATGGCGGAATTCTTCTTGCTTGCCTTTGAGTTTG

Protein sequence

SFFSPMAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL

Homology

[Close]

BLAST of MC01g0330 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.7e-118
Identity = 226/428 (52.80%), Postives = 304/428 (71.03%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           E++ P FFLCPISL IMKDPV V++GITYDRESIEKWLFS K  +CPVT+ V++ +DLTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           NHTLRR+IQ+WCTLNA  G +ER PTPKPP+ ++++ K++ + + S  +Q KCL+RLR I
Sbjct: 64  NHTLRRLIQSWCTLNASYG-IERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI 123

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLG--------------------- 192
            S N TNKRC+E+A V EFLA +V NS++  +S  ++L                      
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 193 ---DEALSILHKLQISEAGLKFLLSNNGGV-FVASLIKVLQNGTYGSRAYAVMLLKSIFE 252
              DEALS+L+ L  SE  LK LL+N  G   V +L K++Q G Y SRAYA +LLK + E
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 243

Query: 253 VADRIQIVSVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVL 312
           VAD +QI+ +  E F E++Q+L DQ S +AT++A+++L+  C   RNR KA++ G + ++
Sbjct: 244 VADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMI 303

Query: 313 IDLLL-DLDSSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEK 372
           I+LL+ D  SSE+R  EM ++ LD+LC CA+GRAE LNH A +AVVSKKILRVS + SE+
Sbjct: 304 IELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSER 363

Query: 373 AVGILCSVAKFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRN 415
           AV +L SV +F A+ S++QEML+LGVV KLC +LQ V  G+K KEKAKE+LKLH R WR 
Sbjct: 364 AVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRE 423

[Close]

BLAST of MC01g0330 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 6.3e-118
Identity = 223/404 (55.20%), Postives = 300/404 (74.26%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           E++ PPFFLCPISL+IMKDPV V++GITYDR+SIEKWLF+ K  +CPVT+  ++++DLTP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           NHTLRR+IQ+WCTLNA  G VER PTP+PP+ ++++ K++ D   S  +Q KCL+RLR I
Sbjct: 69  NHTLRRLIQSWCTLNASYG-VERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 128

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAGLKFLL 192
            S N TNKRC+E+AGV EFLA +V N      S   +L DEAL++L+ L+ SE  LK LL
Sbjct: 129 VSENATNKRCLEAAGVPEFLANIVSN-----DSENGSLTDEALNLLYHLETSETVLKNLL 188

Query: 193 SN-NGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIEIVQVLKD 252
           +N      V SL K++Q G Y SR YA +LLK+I EVAD +Q +++  E F E+VQ+L D
Sbjct: 189 NNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDD 248

Query: 253 QFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD-SSEKRACEMILMALD 312
           + SQ+ATKAA+ +L+ +C   RNR KA++AG + V+I+LL+D   +SE+R  EM ++ LD
Sbjct: 249 RISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLD 308

Query: 313 LLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLRL 372
           LLC CA+GRAE LNH A +AVV KKILRVS   S++AV +L SV +F A+ +++ EML+L
Sbjct: 309 LLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQL 368

Query: 373 GVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 415
           GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP ++
Sbjct: 369 GVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNM 405

[Close]

BLAST of MC01g0330 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 8.3e-62
Identity = 170/450 (37.78%), Postives = 243/450 (54.00%), Query Frame = 0


Query: 8   EDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLS- 67
           +  + E++ P +F+CPISL+IMKDPVT  SGITYDR++I KWL  +K  +CPVT+  L  
Sbjct: 2   DQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPVTKQPLPL 61

Query: 68  NSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQ---- 127
           +SDLTPNH LRR+IQ WC  N   G V R  TP+ P  +  VV+ +   NL  F Q    
Sbjct: 62  DSDLTPNHMLRRLIQHWCVENETRG-VVRISTPRVPPGKLNVVEEIK--NLKKFGQEALG 121

Query: 128 -QKCLQRLRSIASANETNKRCMESAGVVE--FLAGVVCNSINVESSYQANLGDEALSILH 187
            ++ LQ+L  +A  +  N+R M   GV +   L  V C S + +   +    DE+L +LH
Sbjct: 122 REETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLH 181

Query: 188 KLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTA 247
            + I     K +L  N  V + SL  VL    + S+AY ++LL+++ E      +  +  
Sbjct: 182 LIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNP 241

Query: 248 EFFIEIVQVLKDQFS-----------------------------------QQATKAALKL 307
           E F  I+  LKD  +                                   +QA  AAL +
Sbjct: 242 EIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMI 301

Query: 308 LITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEMILMALDLLCGCADGRAELLN 367
           L+   S  RNR   +  GAV  LI+L +   + EKR  E++L  L  LC CA+GRAE+L 
Sbjct: 302 LLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCANGRAEILA 361

Query: 368 HAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLRLGVVTKLCFLLQVVG 415
           H  G+AVV+K++LRVS    ++A+ IL +V+KFS    VV+EM+ +G V KLC +L  + 
Sbjct: 362 HRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLG-MD 421

[Close]

BLAST of MC01g0330 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.9e-45
Identity = 142/402 (35.32%), Postives = 215/402 (53.48%), Query Frame = 0


Query: 14  VDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTPN 73
           +  P  F CPISL++M+DPVTV +G TYDR SIE W+    +TTCPVT+  LS+  L PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 74  HTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILAD------VNLSPFSQQKCLQ 133
           HTLRR+IQ WC  N RS  VER PTPK P D T V  +L+        ++S  S+   L+
Sbjct: 72  HTLRRLIQEWCVAN-RSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 131

Query: 134 RLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAG 193
           RLR  A  ++ N+  + +    E L  ++        +  + L  E+L++L  L I+E  
Sbjct: 132 RLRGFARDSDKNRVLIAAHNATEILIKIL-----FSETTSSELVSESLALLVMLPITEPN 191

Query: 194 LKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSI---FEVADRIQIVSVTAEFFIE 253
               +S++ G  V  L ++L + +  +R  A  L++ +    + AD    +S +   F  
Sbjct: 192 QFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEG 251

Query: 254 IVQVLKDQF-SQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLID-LLLDLDSSEKRAC 313
           ++ +L++   S++A K  +K L  LCS    R  AI AGA  +LID L  D D  +    
Sbjct: 252 VLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD---T 311

Query: 314 EMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQS 373
           E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A + 
Sbjct: 312 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTAEER 371

Query: 374 VVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTW 405
             +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 372 WREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of MC01g0330 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.5e-44
Identity = 141/406 (34.73%), Postives = 215/406 (52.96%), Query Frame = 0


Query: 14  VDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTPN 73
           +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  +TTCPVT+V LS+  L PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPN 71

Query: 74  HTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILAD------VNLSPFSQQKCLQ 133
           HTLRR+IQ WC  N RS  VER PTPK P D   V  +L+        ++S  S+   ++
Sbjct: 72  HTLRRLIQEWCVAN-RSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 131

Query: 134 RLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAG 193
           RLR +A  +E N+  +      E L  ++   I   +S  + L  E+L++L  L ++E  
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRILFADIET-TSLSSELVSESLALLVLLHMTETE 191

Query: 194 LKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVA---DRIQIVSVTAEFFIE 253
            + + S+     V  + ++L + +   R  A  L++ +   A   D   I+S +   F  
Sbjct: 192 CEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 254 IVQVLKDQF-SQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLID-LLLDLDSSEKRAC 313
           ++ +LK+   S++A K  +K +  LC   + R  AI AGA  +LID L  D D  +    
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD---T 311

Query: 314 EMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQS 373
           E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A + 
Sbjct: 312 ERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTAEER 371

Query: 374 VVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSS 409
              E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 372 CRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 406

[Close]

BLAST of MC01g0330 vs. NCBI nr
Match: XP_023543358.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 646 bits (1667), Expect = 1.02e-230
Identity = 335/419 (79.95%), Postives = 379/419 (90.45%), Query Frame = 0


Query: 6   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 65
           MAE +Q E+D PPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSD+H TCPVTQVVL
Sbjct: 1   MAELDQ-ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDRHKTCPVTQVVL 60

Query: 66  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 125
           S+ D+TPNHTLRRVIQAWCT+NA  G +ER PTPKPPVD  QVV+ILAD  LSPFSQQ C
Sbjct: 61  SSFDITPNHTLRRVIQAWCTVNACKG-IERIPTPKPPVDCKQVVRILADAKLSPFSQQNC 120

Query: 126 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ----------ANLGDEAL 185
           L+RLRSIA+ +E+NKRCME+AG VEFLAG+VCNS NVE S +          ANLGDEAL
Sbjct: 121 LRRLRSIAAGSESNKRCMEAAGAVEFLAGIVCNSTNVEFSSEDYAYLIDDQFANLGDEAL 180

Query: 186 SILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIV 245
           SIL+KLQISE+GLKFLL+NNGG+FV++L K+LQNGTYGSRAY+VMLL S+FEVAD+IQI+
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 246 SVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD 305
           S+  +FF+EIVQ+LKDQ S+QA+K+ALKLLI LCSS RNRVKA++AGAVPV+IDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVMIDLLLDLD 300

Query: 306 SSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVA 365
           SSEKR+CEMIL+ LDLLCGCADGRAE+LNHAAG+AVVSKKILRVS LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 366 KFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           +FSAS+SVVQEM+RLGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLP SL
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 417

[Close]

BLAST of MC01g0330 vs. NCBI nr
Match: XP_022925148.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata])

HSP 1 Score: 646 bits (1666), Expect = 1.08e-230
Identity = 337/410 (82.20%), Postives = 368/410 (89.76%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           EVD PPFFLCPISLQIMKDPVTVASGITYDRE+IE+WLFSDKHTTCPVTQVVLS+ D+TP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           N+TL R+IQAWCT+N R   VER PTPKPPVD+ QVVKILADV LSPFSQQ CL+RL SI
Sbjct: 63  NNTLSRLIQAWCTVNNR---VERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESI 122

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ--------ANLGDEALSILHKLQIS 192
           ASANE NKRCME+AG VEFL GVVCNSI +ESS+         ANLGDEALSIL+KLQIS
Sbjct: 123 ASANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAFDDPLANLGDEALSILYKLQIS 182

Query: 193 EAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIE 252
           E+GLKFLL+NNGG+FV +L K LQNGTYGSRAYA MLL+S+FEVAD IQI S+  EFF+E
Sbjct: 183 ESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVE 242

Query: 253 IVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEM 312
           IVQ+LKDQ S+QA+K+ALKLLI LCSSFRNRV A+QAGAVPVLIDLLLDLDSSEKR CEM
Sbjct: 243 IVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEM 302

Query: 313 ILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVV 372
           IL+ALDLLCGCADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVV
Sbjct: 303 ILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVV 362

Query: 373 QEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           QEM RLGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC P SL
Sbjct: 363 QEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSL 409

[Close]

BLAST of MC01g0330 vs. NCBI nr
Match: XP_022978192.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima])

HSP 1 Score: 645 bits (1665), Expect = 2.06e-230
Identity = 334/419 (79.71%), Postives = 379/419 (90.45%), Query Frame = 0


Query: 6   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 65
           MAE +Q E+D PPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSD+H TCPVTQVVL
Sbjct: 1   MAELDQ-ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVL 60

Query: 66  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 125
           S+ D+TPNHT+RRVIQAWCT+NA  G +ER PTPKPP+D  QVV+ILAD  LSPFSQQ C
Sbjct: 61  SSFDITPNHTIRRVIQAWCTINACKG-IERIPTPKPPIDCKQVVRILADAKLSPFSQQNC 120

Query: 126 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ----------ANLGDEAL 185
           L+RLRSIA+A+E+NKRCME+AG VEFLAG+VCNS NVE S +          ANLGDEAL
Sbjct: 121 LRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 186 SILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIV 245
           SIL+KLQISE+GLKFLL+NNGG+FV++L K+LQNGTYGSRAY+VMLL S+FEVAD+IQI+
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 246 SVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD 305
           S+  +FFIEIVQ+LKDQ S+QA+K+ALKLLI LCSS RNRVKA+QAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 306 SSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVA 365
           SSEKR+CEMIL+ LDLLC CADGRAE+LNHAAG+AVVSKKILRVS LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 366 KFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           +FSAS+SVVQEM+RLGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLP SL
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 417

[Close]

BLAST of MC01g0330 vs. NCBI nr
Match: XP_023518739.1 (E3 ubiquitin-protein ligase PUB22-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642 bits (1656), Expect = 3.21e-229
Identity = 333/410 (81.22%), Postives = 369/410 (90.00%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           E+D PPFFLCPISLQIMKDPVTVASGITYDRE+IE+WLFSDKHTTCPVTQVVLS+ D+TP
Sbjct: 3   EIDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           N+TL R+IQAWCT+N R   VER PTPKPPVD+ Q+VKILADV LSPFSQQ CL+RL SI
Sbjct: 63  NNTLSRLIQAWCTVNNR---VERIPTPKPPVDKKQIVKILADVKLSPFSQQNCLRRLGSI 122

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ--------ANLGDEALSILHKLQIS 192
           ASANE NKRCME+AG VEFL GVVCNSI +ESS+Q        ANLGD+ALSIL+KLQIS
Sbjct: 123 ASANEMNKRCMEAAGAVEFLVGVVCNSITIESSFQDFTFDDPLANLGDKALSILYKLQIS 182

Query: 193 EAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIE 252
           E+GLKFLL+NNGG+FV +L K LQNGTYGSRAYA MLL+S+FEVAD IQI ++  EFF+E
Sbjct: 183 ESGLKFLLANNGGIFVKTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRTLNLEFFVE 242

Query: 253 IVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEM 312
           IVQ+LKDQ S+QA+K+ALKLLI LCSSFRNRV A+QAGAVPVLIDLLLDLDSSEKR CE+
Sbjct: 243 IVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEI 302

Query: 313 ILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVV 372
           IL+ALDLLCGCADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVV
Sbjct: 303 ILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVV 362

Query: 373 QEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           QEM RLGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC P SL
Sbjct: 363 QEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSL 409

[Close]

BLAST of MC01g0330 vs. NCBI nr
Match: KAG7033684.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 642 bits (1656), Expect = 4.82e-229
Identity = 334/419 (79.71%), Postives = 377/419 (89.98%), Query Frame = 0


Query: 6   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 65
           MAE +Q E+D PPFFLCPISLQIMKDPVTVASGITYDR+SIEKWLFSD H TCPVTQVVL
Sbjct: 1   MAELDQ-ELDPPPFFLCPISLQIMKDPVTVASGITYDRKSIEKWLFSDGHKTCPVTQVVL 60

Query: 66  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 125
           S+ D+TPN TLRRVIQAWCT+NA  G +ER PTPKPPVD  QVV+ILAD  LSPFSQQ C
Sbjct: 61  SSFDITPNITLRRVIQAWCTVNACKG-IERIPTPKPPVDCKQVVRILADAKLSPFSQQNC 120

Query: 126 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ----------ANLGDEAL 185
           L+RLRSIA+A+E+NKRCME+AG VEFLAG+VCNS NVE S +          ANLGDEAL
Sbjct: 121 LRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGDEAL 180

Query: 186 SILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIV 245
           SILHKLQISE+GLKFLL+NNGG+FV++L K+LQNGTYGSRAY+VMLL S+FEVAD+IQI+
Sbjct: 181 SILHKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 246 SVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD 305
           S+  +FF+EIVQ+LKDQ S+QA+K+ALKLLI LCSS RNRVKA++AGAVPVLIDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 306 SSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVA 365
           SSEKR+CEMIL+ LDLLCGCADGRAE+LNHAAG+A+VSKKILRVS LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIA 360

Query: 366 KFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           +FSAS+SVVQEM+RLGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLP SL
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 417

[Close]

BLAST of MC01g0330 vs. ExPASy TrEMBL
Match: A0A6J1EEF1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478 PE=4 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 5.22e-231
Identity = 337/410 (82.20%), Postives = 368/410 (89.76%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           EVD PPFFLCPISLQIMKDPVTVASGITYDRE+IE+WLFSDKHTTCPVTQVVLS+ D+TP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFSDKHTTCPVTQVVLSDFDITP 62

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           N+TL R+IQAWCT+N R   VER PTPKPPVD+ QVVKILADV LSPFSQQ CL+RL SI
Sbjct: 63  NNTLSRLIQAWCTVNNR---VERIPTPKPPVDKKQVVKILADVKLSPFSQQNCLRRLESI 122

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ--------ANLGDEALSILHKLQIS 192
           ASANE NKRCME+AG VEFL GVVCNSI +ESS+         ANLGDEALSIL+KLQIS
Sbjct: 123 ASANEMNKRCMEAAGAVEFLVGVVCNSITIESSFPDFAFDDPLANLGDEALSILYKLQIS 182

Query: 193 EAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIE 252
           E+GLKFLL+NNGG+FV +L K LQNGTYGSRAYA MLL+S+FEVAD IQI S+  EFF+E
Sbjct: 183 ESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVE 242

Query: 253 IVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEM 312
           IVQ+LKDQ S+QA+K+ALKLLI LCSSFRNRV A+QAGAVPVLIDLLLDLDSSEKR CEM
Sbjct: 243 IVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDSSEKRLCEM 302

Query: 313 ILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVV 372
           IL+ALDLLCGCADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVV
Sbjct: 303 ILVALDLLCGCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVV 362

Query: 373 QEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           QEM RLGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC P SL
Sbjct: 363 QEMARLGVVVKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSL 409

[Close]

BLAST of MC01g0330 vs. ExPASy TrEMBL
Match: A0A6J1IKG7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 PE=4 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 9.95e-231
Identity = 334/419 (79.71%), Postives = 379/419 (90.45%), Query Frame = 0


Query: 6   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 65
           MAE +Q E+D PPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSD+H TCPVTQVVL
Sbjct: 1   MAELDQ-ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPVTQVVL 60

Query: 66  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 125
           S+ D+TPNHT+RRVIQAWCT+NA  G +ER PTPKPP+D  QVV+ILAD  LSPFSQQ C
Sbjct: 61  SSFDITPNHTIRRVIQAWCTINACKG-IERIPTPKPPIDCKQVVRILADAKLSPFSQQNC 120

Query: 126 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ----------ANLGDEAL 185
           L+RLRSIA+A+E+NKRCME+AG VEFLAG+VCNS NVE S +          ANLGDEAL
Sbjct: 121 LRRLRSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQFANLGDEAL 180

Query: 186 SILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIV 245
           SIL+KLQISE+GLKFLL+NNGG+FV++L K+LQNGTYGSRAY+VMLL S+FEVAD+IQI+
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRAYSVMLLTSMFEVADQIQIL 240

Query: 246 SVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD 305
           S+  +FFIEIVQ+LKDQ S+QA+K+ALKLLI LCSS RNRVKA+QAGA+PVLIDLLLDLD
Sbjct: 241 SLNTDFFIEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVQAGAIPVLIDLLLDLD 300

Query: 306 SSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVA 365
           SSEKR+CEMIL+ LDLLC CADGRAE+LNHAAG+AVVSKKILRVS LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCRCADGRAEVLNHAAGIAVVSKKILRVSTLGSEKAVGILCSIA 360

Query: 366 KFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           +FSAS+SVVQEM+RLGVVTKLCFLLQVVGAG KAKEKAKEILKLHGRTWRNSSCLP SL
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVVGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 417

[Close]

BLAST of MC01g0330 vs. ExPASy TrEMBL
Match: A0A6J1GGQ8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790 PE=4 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 7.76e-228
Identity = 331/419 (79.00%), Postives = 376/419 (89.74%), Query Frame = 0


Query: 6   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 65
           MAE +Q E+D PPFFLCPISLQIMKDPVTVASGITYDRESIE+WLFSD+H TCP+TQVVL
Sbjct: 1   MAELDQ-ELDPPPFFLCPISLQIMKDPVTVASGITYDRESIERWLFSDRHKTCPITQVVL 60

Query: 66  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 125
           SN D+TPNHTLRRVIQAWCT+NA  G VER PTPKPPVD  QVV+ILAD  LS FSQQ C
Sbjct: 61  SNFDITPNHTLRRVIQAWCTVNACKG-VERIPTPKPPVDCKQVVRILADAKLSSFSQQNC 120

Query: 126 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ----------ANLGDEAL 185
           L+RL+SIA+A+E+NKRCME+AG VEFLAG+VCNS NVE S +          ANLG EAL
Sbjct: 121 LRRLQSIAAASESNKRCMEAAGAVEFLAGIVCNSTNVEFSLEDYAYLIDDQLANLGFEAL 180

Query: 186 SILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIV 245
           SIL+KLQISE+GLKFLL+NNGG+FV++L K+LQNGTYGSR Y+VMLL S+FEVAD+IQI+
Sbjct: 181 SILYKLQISESGLKFLLANNGGIFVSTLTKILQNGTYGSRTYSVMLLTSMFEVADQIQIL 240

Query: 246 SVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD 305
           S+  +FF+EIVQ+LKDQ S+QA+K+ALKLLI LCSS RNRVKA++AGAVPVLIDLLLDLD
Sbjct: 241 SLNTDFFVEIVQILKDQISKQASKSALKLLIGLCSSSRNRVKAVKAGAVPVLIDLLLDLD 300

Query: 306 SSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVA 365
           SSEKR+CEMIL+ LDLLCGCADGRAE+LNHAAG+A+VSKKILRVS LGSEKAVGILCS+A
Sbjct: 301 SSEKRSCEMILVTLDLLCGCADGRAEVLNHAAGIAIVSKKILRVSTLGSEKAVGILCSIA 360

Query: 366 KFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           +FSAS+SVVQEM+RLGVVTKLCFLLQV GAG KAKEKAKEILKLHGRTWRNSSCLP SL
Sbjct: 361 RFSASESVVQEMMRLGVVTKLCFLLQVAGAGGKAKEKAKEILKLHGRTWRNSSCLPSSL 417

[Close]

BLAST of MC01g0330 vs. ExPASy TrEMBL
Match: A0A6J1HP11 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 PE=4 SV=1)

HSP 1 Score: 635 bits (1639), Expect = 6.72e-227
Identity = 330/410 (80.49%), Postives = 365/410 (89.02%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           EVD PPFFLCPISLQIMKDPVTVASGITYDRE+IE+WLF DKHTTCPVTQVVLS+ D+TP
Sbjct: 3   EVDPPPFFLCPISLQIMKDPVTVASGITYDRENIERWLFFDKHTTCPVTQVVLSDFDITP 62

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           N+TL R+IQAW TLN     V+R PTPKPP+D+ QV+KILA+V LSPFSQQ CLQRLRSI
Sbjct: 63  NNTLSRLIQAWSTLN---DGVDRIPTPKPPIDKKQVIKILANVKLSPFSQQNCLQRLRSI 122

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ--------ANLGDEALSILHKLQIS 192
           AS NETNKRCME+AG VEFL GV CNSI +ES++Q        ANLGDEALSIL+KLQIS
Sbjct: 123 ASTNETNKRCMEAAGAVEFLVGVACNSITIESNFQDFAFDDPLANLGDEALSILYKLQIS 182

Query: 193 EAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIE 252
           E+GLKFLL+NNGG+FV +L K LQNGTYGSRAYA MLL+S+FEVAD IQI S+  EFF+E
Sbjct: 183 ESGLKFLLANNGGIFVTTLTKFLQNGTYGSRAYATMLLQSMFEVADEIQIRSLNLEFFVE 242

Query: 253 IVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEM 312
           IVQ+LKDQ S+QA+K+ALKLLI LCSSFRNRV A+QAGAVPVLIDLLLDLD SEKR CEM
Sbjct: 243 IVQILKDQISKQASKSALKLLIGLCSSFRNRVNAVQAGAVPVLIDLLLDLDLSEKRLCEM 302

Query: 313 ILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVV 372
           IL+ALDLLC CADGRAELLNHAAG+AVVSKKILRVS LGSEKAVGILCS+A+FSASQSVV
Sbjct: 303 ILVALDLLCSCADGRAELLNHAAGIAVVSKKILRVSSLGSEKAVGILCSIARFSASQSVV 362

Query: 373 QEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           QEM+RLGVV KLCFLLQVVGAG+KAKEKAKEILKLHGRTWRNSSC P SL
Sbjct: 363 QEMVRLGVVMKLCFLLQVVGAGAKAKEKAKEILKLHGRTWRNSSCFPSSL 409

[Close]

BLAST of MC01g0330 vs. ExPASy TrEMBL
Match: A0A6J1DUC4 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111023178 PE=4 SV=1)

HSP 1 Score: 625 bits (1611), Expect = 1.37e-222
Identity = 345/417 (82.73%), Postives = 345/417 (82.73%), Query Frame = 0


Query: 6   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 65
           MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL
Sbjct: 1   MAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVL 60

Query: 66  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 125
           SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC
Sbjct: 61  SNSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKC 120

Query: 126 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ--------ANLGDEALSI 185
           LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQ        ANLGDEALSI
Sbjct: 121 LQRLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQDYAFDDELANLGDEALSI 180

Query: 186 LHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSV 245
           LHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSV
Sbjct: 181 LHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSV 240

Query: 246 TAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSS 305
           TAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAV             
Sbjct: 241 TAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVXXXXXXXXXXXXX 300

Query: 306 EKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKF 365
                                                              GILCSVAKF
Sbjct: 301 XXXXXXXXXXXXX-----XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGILCSVAKF 360

Query: 366 SASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 414
           SASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL
Sbjct: 361 SASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 412

[Close]

BLAST of MC01g0330 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 427.6 bits (1098), Expect = 1.2e-119
Identity = 226/428 (52.80%), Postives = 304/428 (71.03%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           E++ P FFLCPISL IMKDPV V++GITYDRESIEKWLFS K  +CPVT+ V++ +DLTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           NHTLRR+IQ+WCTLNA  G +ER PTPKPP+ ++++ K++ + + S  +Q KCL+RLR I
Sbjct: 64  NHTLRRLIQSWCTLNASYG-IERIPTPKPPICKSEIEKLIKESSSSHLNQVKCLKRLRQI 123

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLG--------------------- 192
            S N TNKRC+E+A V EFLA +V NS++  +S  ++L                      
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 193 ---DEALSILHKLQISEAGLKFLLSNNGGV-FVASLIKVLQNGTYGSRAYAVMLLKSIFE 252
              DEALS+L+ L  SE  LK LL+N  G   V +L K++Q G Y SRAYA +LLK + E
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALLLKKLLE 243

Query: 253 VADRIQIVSVTAEFFIEIVQVLKDQFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVL 312
           VAD +QI+ +  E F E++Q+L DQ S +AT++A+++L+  C   RNR KA++ G + ++
Sbjct: 244 VADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISMI 303

Query: 313 IDLLL-DLDSSEKRACEMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEK 372
           I+LL+ D  SSE+R  EM ++ LD+LC CA+GRAE LNH A +AVVSKKILRVS + SE+
Sbjct: 304 IELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSER 363

Query: 373 AVGILCSVAKFSASQSVVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRN 415
           AV +L SV +F A+ S++QEML+LGVV KLC +LQ V  G+K KEKAKE+LKLH R WR 
Sbjct: 364 AVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQ-VSCGNKTKEKAKELLKLHARVWRE 423

[Close]

BLAST of MC01g0330 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 425.6 bits (1093), Expect = 4.5e-119
Identity = 223/404 (55.20%), Postives = 300/404 (74.26%), Query Frame = 0


Query: 13  EVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTP 72
           E++ PPFFLCPISL+IMKDPV V++GITYDR+SIEKWLF+ K  +CPVT+  ++++DLTP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 73  NHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSPFSQQKCLQRLRSI 132
           NHTLRR+IQ+WCTLNA  G VER PTP+PP+ ++++ K++ D   S  +Q KCL+RLR I
Sbjct: 69  NHTLRRLIQSWCTLNASYG-VERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 128

Query: 133 ASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAGLKFLL 192
            S N TNKRC+E+AGV EFLA +V N      S   +L DEAL++L+ L+ SE  LK LL
Sbjct: 129 VSENATNKRCLEAAGVPEFLANIVSN-----DSENGSLTDEALNLLYHLETSETVLKNLL 188

Query: 193 SN-NGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRIQIVSVTAEFFIEIVQVLKD 252
           +N      V SL K++Q G Y SR YA +LLK+I EVAD +Q +++  E F E+VQ+L D
Sbjct: 189 NNKKDNNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDD 248

Query: 253 QFSQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLD-SSEKRACEMILMALD 312
           + SQ+ATKAA+ +L+ +C   RNR KA++AG + V+I+LL+D   +SE+R  EM ++ LD
Sbjct: 249 RISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLD 308

Query: 313 LLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLRL 372
           LLC CA+GRAE LNH A +AVV KKILRVS   S++AV +L SV +F A+ +++ EML+L
Sbjct: 309 LLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQL 368

Query: 373 GVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSSCLPLSL 415
           GVV KLC +LQ V  G K KEKAKE+LKLH R W++S CLP ++
Sbjct: 369 GVVAKLCLVLQ-VSCGGKTKEKAKELLKLHARVWKDSPCLPKNM 405

[Close]

BLAST of MC01g0330 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 243.0 bits (619), Expect = 4.1e-64
Identity = 172/457 (37.64%), Postives = 246/457 (53.83%), Query Frame = 0


Query: 1   SFFSPMAEDNQVEVDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPV 60
           S+  P  +  + E++ P +F+CPISL+IMKDPVT  SGITYDR++I KWL  +K  +CPV
Sbjct: 9   SYVFPQMDQEEEEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL--EKVPSCPV 68

Query: 61  TQVVLS-NSDLTPNHTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILADVNLSP 120
           T+  L  +SDLTPNH LRR+IQ WC  N   G V R  TP+ P  +  VV+ +   NL  
Sbjct: 69  TKQPLPLDSDLTPNHMLRRLIQHWCVENETRG-VVRISTPRVPPGKLNVVEEIK--NLKK 128

Query: 121 FSQ-----QKCLQRLRSIASANETNKRCMESAGVVE--FLAGVVCNSINVESSYQANLGD 180
           F Q     ++ LQ+L  +A  +  N+R M   GV +   L  V C S + +   +    D
Sbjct: 129 FGQEALGREETLQKLEVLA-MDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLD 188

Query: 181 EALSILHKLQISEAGLKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVADRI 240
           E+L +LH + I     K +L  N  V + SL  VL    + S+AY ++LL+++ E     
Sbjct: 189 ESLRLLHLIGIPSNDAKTILMENDRV-MESLTWVLHQEDFLSKAYTIVLLRNLTEYTSSH 248

Query: 241 QIVSVTAEFFIEIVQVLKDQFS-----------------------------------QQA 300
            +  +  E F  I+  LKD  +                                   +QA
Sbjct: 249 IVERLNPEIFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQA 308

Query: 301 TKAALKLLITLCSSFRNRVKAIQAGAVPVLIDLLLDLDSSEKRACEMILMALDLLCGCAD 360
             AAL +L+   S  RNR   +  GAV  LI+L +   + EKR  E++L  L  LC CA+
Sbjct: 309 VTAALMILLETSSWSRNRSLLVDLGAVSELIELEISY-TGEKRITELMLGVLSRLCCCAN 368

Query: 361 GRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQSVVQEMLRLGVVTKLC 415
           GRAE+L H  G+AVV+K++LRVS    ++A+ IL +V+KFS    VV+EM+ +G V KLC
Sbjct: 369 GRAEILAHRGGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLC 428

[Close]

BLAST of MC01g0330 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-46
Identity = 142/402 (35.32%), Postives = 215/402 (53.48%), Query Frame = 0


Query: 14  VDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTPN 73
           +  P  F CPISL++M+DPVTV +G TYDR SIE W+    +TTCPVT+  LS+  L PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 74  HTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILAD------VNLSPFSQQKCLQ 133
           HTLRR+IQ WC  N RS  VER PTPK P D T V  +L+        ++S  S+   L+
Sbjct: 72  HTLRRLIQEWCVAN-RSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALR 131

Query: 134 RLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAG 193
           RLR  A  ++ N+  + +    E L  ++        +  + L  E+L++L  L I+E  
Sbjct: 132 RLRGFARDSDKNRVLIAAHNATEILIKIL-----FSETTSSELVSESLALLVMLPITEPN 191

Query: 194 LKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSI---FEVADRIQIVSVTAEFFIE 253
               +S++ G  V  L ++L + +  +R  A  L++ +    + AD    +S +   F  
Sbjct: 192 QFVSISSDPG-RVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEG 251

Query: 254 IVQVLKDQF-SQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLID-LLLDLDSSEKRAC 313
           ++ +L++   S++A K  +K L  LCS    R  AI AGA  +LID L  D D  +    
Sbjct: 252 VLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCD---T 311

Query: 314 EMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQS 373
           E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A + 
Sbjct: 312 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGAL--LALCTAEER 371

Query: 374 VVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTW 405
             +E    GVV +L  ++Q      +AK+KA+++LKL   +W
Sbjct: 372 WREEAAGAGVVVQLLLMVQ-SECTERAKKKAQKLLKLLRDSW 400

[Close]

BLAST of MC01g0330 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 180.6 bits (457), Expect = 2.5e-45
Identity = 141/406 (34.73%), Postives = 215/406 (52.96%), Query Frame = 0


Query: 14  VDTPPFFLCPISLQIMKDPVTVASGITYDRESIEKWLFSDKHTTCPVTQVVLSNSDLTPN 73
           +  P  F CPISL +M DPVT+++G TYDR SI+ W+ +  +TTCPVT+V LS+  L PN
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWI-AMGNTTCPVTRVALSDFTLIPN 71

Query: 74  HTLRRVIQAWCTLNARSGSVERFPTPKPPVDRTQVVKILAD------VNLSPFSQQKCLQ 133
           HTLRR+IQ WC  N RS  VER PTPK P D   V  +L+        ++S  S+   ++
Sbjct: 72  HTLRRLIQEWCVAN-RSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIR 131

Query: 134 RLRSIASANETNKRCMESAGVVEFLAGVVCNSINVESSYQANLGDEALSILHKLQISEAG 193
           RLR +A  +E N+  +      E L  ++   I   +S  + L  E+L++L  L ++E  
Sbjct: 132 RLRGLARDSEKNRVLIAGHNAREILVRILFADIET-TSLSSELVSESLALLVLLHMTETE 191

Query: 194 LKFLLSNNGGVFVASLIKVLQNGTYGSRAYAVMLLKSIFEVA---DRIQIVSVTAEFFIE 253
            + + S+     V  + ++L + +   R  A  L++ +   A   D   I+S +   F  
Sbjct: 192 CEAVASDPSR--VGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEG 251

Query: 254 IVQVLKDQF-SQQATKAALKLLITLCSSFRNRVKAIQAGAVPVLID-LLLDLDSSEKRAC 313
           ++ +LK+   S++A K  +K +  LC   + R  AI AGA  +LID L  D D  +    
Sbjct: 252 VLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCD---T 311

Query: 314 EMILMALDLLCGCADGRAELLNHAAGLAVVSKKILRVSWLGSEKAVGILCSVAKFSASQS 373
           E  L  ++LLC   +G A    HA  + ++ K ILRVS   +E A G L  +A  +A + 
Sbjct: 312 ERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL--LALCTAEER 371

Query: 374 VVQEMLRLGVVTKLCFLLQVVGAGSKAKEKAKEILKLHGRTWRNSS 409
              E    G+VT+L  L+Q      +AK KA+ +LKL   +W + S
Sbjct: 372 CRDEAAAAGLVTQLLLLVQ-SDCTERAKRKAQMLLKLLRDSWPDDS 406

The following BLAST results are available for this feature:

BLAST of MC01g0330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVC6	1.7e-118	52.80	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q84TG3	6.3e-118	55.20	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SF15	8.3e-62	37.78	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	1.9e-45	35.32	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9FXA4	3.5e-44	34.73	U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2...	[more]

BLAST of MC01g0330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023543358.1	1.02e-230	79.95	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]
XP_022925148.1	1.08e-230	82.20	E3 ubiquitin-protein ligase PUB22-like [Cucurbita moschata]	[more]
XP_022978192.1	2.06e-230	79.71	E3 ubiquitin-protein ligase PUB22-like [Cucurbita maxima]	[more]
XP_023518739.1	3.21e-229	81.22	E3 ubiquitin-protein ligase PUB22-like, partial [Cucurbita pepo subsp. pepo]	[more]
KAG7033684.1	4.82e-229	79.71	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of MC01g0330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EEF1	5.22e-231	82.20	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432478...	[more]
A0A6J1IKG7	9.95e-231	79.71	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111478249 P...	[more]
A0A6J1GGQ8	7.76e-228	79.00	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111453790...	[more]
A0A6J1HP11	6.72e-227	80.49	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466021 P...	[more]
A0A6J1DUC4	1.37e-222	82.73	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102317...	[more]

BLAST of MC01g0330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52450.1	1.2e-119	52.80	plant U-box 22	[more]
AT2G35930.1	4.5e-119	55.20	plant U-box 23	[more]
AT3G11840.1	4.1e-64	37.64	plant U-box 24	[more]
AT3G19380.1	1.3e-46	35.32	plant U-box 25	[more]
AT1G49780.1	2.5e-45	34.73	plant U-box 26	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 19..83 e-value: 2.7E-33 score: 126.6
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 17..85 e-value: 1.1E-21 score: 76.8
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 15..90 score: 44.239113
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 5..95 e-value: 1.9E-29 score: 103.6
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 106..402 e-value: 1.3E-16 score: 62.4
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 13..413
None	No IPR available	PANTHER	PTHR22849:SF132	E3 UBIQUITIN-PROTEIN LIGASE PUB23	coord: 13..413
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 20..62 e-value: 1.92006E-22 score: 87.289
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 8..87
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 109..381

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0330.1	MC01g0330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity