MC01g0284 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0284
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	protein PNS1-like
Location	MC01: 9467253 .. 9469288 (+)
RNA-Seq Expression	MC01g0284
Synteny	MC01g0284

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATGATTAGTCTAAGCAGACTTGAAAGTCCTCTGCATTTTTTTGATAGGTGTGGAAAATCCAGAGCATTTAATGTTGTTGATTCTTGAGGGCCAAGAATTTCCAAGAAGGTTTACTTGCTCCACAACCAATCCAAAGCACTTCTGAAGAAAGCAACTGCCGCCAACTGCTGCTGTTGATGAATTAGGAACTGCTTTTTCTCCTGGCAAGAATGGAAACAAGAACACCATCAGTTTTACAGAATCCCCACCAAGTTCAAGAAAGAAGAGTTCCATCTTCAACGCCCACCAAGGTCTCCTTTTTCTCTCTCTAAAAAGCACTTCCATTTTCATATTCATGTCAATTCAGATCCATCTTTGGTATATGGAGGTTGACTTACATGTATATGGATGAATGATGAATCTTGTTTGTTGTAGTTGCAGGTTCAAGAACCGAGGGTTCTGCCGGTTGAGCCTACGGCGGGGTGGAGATTTTTCAGGTGGCTTTGCAGCCTGGTTTTCTACATCCATTTATGTCTGGTAGGTGTCTTGGTGATCTTTCTGGTAGTTCAGGGGCTTCGGTCGGCTTCTAAAACCCATCATTTTCACCCTGAAAAGTGGTATCCGCCGTTGCTGACTGCTACTGGGAGCTCTGGAATCATTGCGTTCTCATGGCAGGGCTTCACTGGCTGTTGCCCTTCTAAAGCCTTCAAGATGGCATTCTGGTTCAGCCCCGTGGTATCGCTTGCTGCTGGCATTTTCTTTGTCATTGTTGGATCTGCGGGCGGTTTAGCAGCGGGAGCAATCCTCATAGTTTCTTCCTTATTTCTGTCGGTATATGTCTGTTGGGTATATCGCCGGTTTAACTATGCGATTCGACTTCTATCGGTTTCTACTGAGTACCCTCCGACAAAAACTCCAATCTTTGTACTTGGGTCGATCCTGGTGGGCATTCTTTACGCCTCTGTTTTGGTCACTGGCATTGGTGGAGCAGTCGCTCTTAGATCTAGTTGGAAAGCAGCGTTCATAGCAGCAATCTTGTTGAGCCTAACCTGGACTCTGCAGGTCATCAAGAACATAGTGCAGGTTACAATCTCGCGCATAAAGTACTTGAATTTAGCTCGGGGCCACGAAAAGGACACACGAGCAGCTTTTCACGACACGATGAAGCATTTAATCGGAACTATTTCGATCGGCTCAGTCATCGCCCCCCTTGTCAGCTTCATTCAGGGATCTGCCCGTGCCATGAGGTTGATGGCAGGAGAATCAGATGAGTTCCTTTTCTCCTGTGCACATTGCTGCTCGGGGATTGCTTCGACGCTGAGAAACCATGGAAACAGGTGGGGTTTCGTCCACGTTGGTGTTTACAATAAAGGGATGGTGCAGGCTTCAGTCGATACCTGGGAGGCATTCAAGAAGGTGGAATTGGAAGCTGTTATAGACTACGATCTCACCGTGTCTTTCTGCTTTCTCTGCGGGGTTTCGGGGGGTGCAATCTGCAGCATTATAAGCGGGATCTGGGCACTCGTAATACACAAGAGCTATGCAACAGAATTAGCAATTTACGCATTCCTGATCGGCTATTTTCTGGTAATGAGCTGAGATATTATAAAAGAAAAAAAAAAAAAAGAACTGTCCTAAACATAAATTTAAGGAAATGGTAATACCTGAGAAGGCATTTAACATCTGGATGACAATGTTCATGGTTGTTGTTGTTGGCAGTGTCGAATCGGAATGGCATGGCCGCAAGCATGTGTTTCTGCCTACTATGTGGCTTATGCAGACAATCCCCAGCATCCTCGGTTCGATTCGATGGTACCACAGCGTATTCGAGAGCTTCAGCGGAGATTGCAAGCTTAATCAACATGCAACTTGGAAACAAGTGAAGAAGCAGATTTAGACTATTCCTCAAGCTCGTTCATGAAGAGACACTGGCATAAGTAAAAAAATGCCCTCTGAGAAACCCACTGAAACGTAAATTCCAGTTGTGATATCCTCTTTCCATTTTCCCTGCAATTTTTCTTTTTTCTTTCAGATTTCTTGTGCAGTAGAGAAAAG

mRNA sequence

ATATGATTAGTCTAAGCAGACTTGAAAGTCCTCTGCATTTTTTTGATAGGTGTGGAAAATCCAGAGCATTTAATGTTGTTGATTCTTGAGGGCCAAGAATTTCCAAGAAGGTTTACTTGCTCCACAACCAATCCAAAGCACTTCTGAAGAAAGCAACTGCCGCCAACTGCTGCTGTTGATGAATTAGGAACTGCTTTTTCTCCTGGCAAGAATGGAAACAAGAACACCATCAGTTTTACAGAATCCCCACCAAGTTCAAGAAAGAAGAGTTCCATCTTCAACGCCCACCAAGGTTCAAGAACCGAGGGTTCTGCCGGTTGAGCCTACGGCGGGGTGGAGATTTTTCAGGTGGCTTTGCAGCCTGGTTTTCTACATCCATTTATGTCTGGTAGGTGTCTTGGTGATCTTTCTGGTAGTTCAGGGGCTTCGGTCGGCTTCTAAAACCCATCATTTTCACCCTGAAAAGTGGTATCCGCCGTTGCTGACTGCTACTGGGAGCTCTGGAATCATTGCGTTCTCATGGCAGGGCTTCACTGGCTGTTGCCCTTCTAAAGCCTTCAAGATGGCATTCTGGTTCAGCCCCGTGGTATCGCTTGCTGCTGGCATTTTCTTTGTCATTGTTGGATCTGCGGGCGGTTTAGCAGCGGGAGCAATCCTCATAGTTTCTTCCTTATTTCTGTCGGTATATGTCTGTTGGGTATATCGCCGGTTTAACTATGCGATTCGACTTCTATCGGTTTCTACTGAGTACCCTCCGACAAAAACTCCAATCTTTGTACTTGGGTCGATCCTGGTGGGCATTCTTTACGCCTCTGTTTTGGTCACTGGCATTGGTGGAGCAGTCGCTCTTAGATCTAGTTGGAAAGCAGCGTTCATAGCAGCAATCTTGTTGAGCCTAACCTGGACTCTGCAGGTCATCAAGAACATAGTGCAGGTTACAATCTCGCGCATAAAGTACTTGAATTTAGCTCGGGGCCACGAAAAGGACACACGAGCAGCTTTTCACGACACGATGAAGCATTTAATCGGAACTATTTCGATCGGCTCAGTCATCGCCCCCCTTGTCAGCTTCATTCAGGGATCTGCCCGTGCCATGAGGTTGATGGCAGGAGAATCAGATGAGTTCCTTTTCTCCTGTGCACATTGCTGCTCGGGGATTGCTTCGACGCTGAGAAACCATGGAAACAGGTGGGGTTTCGTCCACGTTGGTGTTTACAATAAAGGGATGGTGCAGGCTTCAGTCGATACCTGGGAGGCATTCAAGAAGGTGGAATTGGAAGCTGTTATAGACTACGATCTCACCGTGTCTTTCTGCTTTCTCTGCGGGGTTTCGGGGGGTGCAATCTGCAGCATTATAAGCGGGATCTGGGCACTCGTAATACACAAGAGCTATGCAACAGAATTAGCAATTTACGCATTCCTGATCGGCTATTTTCTGTGTCGAATCGGAATGGCATGGCCGCAAGCATGTGTTTCTGCCTACTATGTGGCTTATGCAGACAATCCCCAGCATCCTCGGTTCGATTCGATGGTACCACAGCGTATTCGAGAGCTTCAGCGGAGATTGCAAGCTTAATCAACATGCAACTTGGAAACAAGTGAAGAAGCAGATTTAGACTATTCCTCAAGCTCGTTCATGAAGAGACACTGGCATAAGTAAAAAAATGCCCTCTGAGAAACCCACTGAAACGTAAATTCCAGTTGTGATATCCTCTTTCCATTTTCCCTGCAATTTTTCTTTTTTCTTTCAGATTTCTTGTGCAGTAGAGAAAAG

Coding sequence (CDS)

ATGGAAACAAGAACACCATCAGTTTTACAGAATCCCCACCAAGTTCAAGAAAGAAGAGTTCCATCTTCAACGCCCACCAAGGTTCAAGAACCGAGGGTTCTGCCGGTTGAGCCTACGGCGGGGTGGAGATTTTTCAGGTGGCTTTGCAGCCTGGTTTTCTACATCCATTTATGTCTGGTAGGTGTCTTGGTGATCTTTCTGGTAGTTCAGGGGCTTCGGTCGGCTTCTAAAACCCATCATTTTCACCCTGAAAAGTGGTATCCGCCGTTGCTGACTGCTACTGGGAGCTCTGGAATCATTGCGTTCTCATGGCAGGGCTTCACTGGCTGTTGCCCTTCTAAAGCCTTCAAGATGGCATTCTGGTTCAGCCCCGTGGTATCGCTTGCTGCTGGCATTTTCTTTGTCATTGTTGGATCTGCGGGCGGTTTAGCAGCGGGAGCAATCCTCATAGTTTCTTCCTTATTTCTGTCGGTATATGTCTGTTGGGTATATCGCCGGTTTAACTATGCGATTCGACTTCTATCGGTTTCTACTGAGTACCCTCCGACAAAAACTCCAATCTTTGTACTTGGGTCGATCCTGGTGGGCATTCTTTACGCCTCTGTTTTGGTCACTGGCATTGGTGGAGCAGTCGCTCTTAGATCTAGTTGGAAAGCAGCGTTCATAGCAGCAATCTTGTTGAGCCTAACCTGGACTCTGCAGGTCATCAAGAACATAGTGCAGGTTACAATCTCGCGCATAAAGTACTTGAATTTAGCTCGGGGCCACGAAAAGGACACACGAGCAGCTTTTCACGACACGATGAAGCATTTAATCGGAACTATTTCGATCGGCTCAGTCATCGCCCCCCTTGTCAGCTTCATTCAGGGATCTGCCCGTGCCATGAGGTTGATGGCAGGAGAATCAGATGAGTTCCTTTTCTCCTGTGCACATTGCTGCTCGGGGATTGCTTCGACGCTGAGAAACCATGGAAACAGGTGGGGTTTCGTCCACGTTGGTGTTTACAATAAAGGGATGGTGCAGGCTTCAGTCGATACCTGGGAGGCATTCAAGAAGGTGGAATTGGAAGCTGTTATAGACTACGATCTCACCGTGTCTTTCTGCTTTCTCTGCGGGGTTTCGGGGGGTGCAATCTGCAGCATTATAAGCGGGATCTGGGCACTCGTAATACACAAGAGCTATGCAACAGAATTAGCAATTTACGCATTCCTGATCGGCTATTTTCTGTGTCGAATCGGAATGGCATGGCCGCAAGCATGTGTTTCTGCCTACTATGTGGCTTATGCAGACAATCCCCAGCATCCTCGGTTCGATTCGATGGTACCACAGCGTATTCGAGAGCTTCAGCGGAGATTGCAAGCTTAA

Protein sequence

METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVIDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA

Homology

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BLAST of MC01g0284 vs. ExPASy Swiss-Prot
Match: Q4I8E9 (Protein PNS1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=PNS1 PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.0e-09
Identity = 62/300 (20.67%), Postives = 124/300 (41.33%), Query Frame = 0


Query: 148 ILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEYPPTK-TPIFVLGSILVGILYASVLVTG 207
           + ++  +  + +  W      YAI L+ +  +Y P +  P    G              G
Sbjct: 238 VSLIGGIIATAFSAW------YAITLVGIYVKYQPAQDNPSCADGG------------CG 297

Query: 208 IGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIVQVTISRIK---YLNLARGHEKDTRAA 267
            G  + L        IA I  ++ W  + +KN +  TI+ +    Y N     +  TRA+
Sbjct: 298 KGKVIGL--------IAFITFAMYWFSEWLKNTIHTTIAGVYGSWYFNPHNFPKDATRAS 357

Query: 268 FHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAGESDEF----LFSCAHCCSGIAST 327
               + +  G+I++GS++  ++ F++    A R   G    F    +F C  C  G+   
Sbjct: 358 AKRALTYSFGSIALGSLLVAIIQFLRQICNAARNQEGADGSFVGYAIFCCISCLLGLLEW 417

Query: 328 LRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVIDYDLTVSFCFLCGVSGGAIC 387
                NR+ F H+ +Y K    A+ DTW+  K   ++A+I+  L         +     C
Sbjct: 418 AVEFINRYAFCHIALYGKAYFAAAKDTWKMIKDRGIDALINDCLIGPVLSFGALFIAYAC 477

Query: 388 SIISGIWALVIHKSYATE------LAIYAFLIGYFLCRIGMAWPQACVSAYYVAYADNPQ 434
           ++++ ++      +Y ++      +  ++FLIG+ +  +      + +   +VA   +PQ
Sbjct: 478 ALLAYLYLYFTDPAYNSDGQYTAVVMAFSFLIGFQIANVFTTPISSGIETIFVAAGWDPQ 511

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BLAST of MC01g0284 vs. ExPASy Swiss-Prot
Match: Q6C938 (Protein PNS1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PNS1 PE=3 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.6e-05
Identity = 52/233 (22.32%), Postives = 107/233 (45.92%), Query Frame = 0


Query: 234 QVIKNIVQVTISRI----KYLNLA-RGHEKD-TRAAFHDTMKHLIGTISIGSVIAPLVSF 293
           +VIKN++ VTIS +     Y + + +G  K    ++F   + + +G+IS+GS+I  +++F
Sbjct: 330 EVIKNVIHVTISGVYGSWYYCSKSDQGMPKHAAMSSFRRAVTYSLGSISLGSLIVSIINF 389

Query: 294 IQGSARAMRLMAGESDE----FLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQAS 353
           I+     ++  A +S +     L     CC G+   L  + N + + ++ +Y K  V ++
Sbjct: 390 IRQILSVLQQDARQSGDTLATVLLCFVQCCFGVLDWLVTYFNHYAYSYIALYGKAYVPSA 449

Query: 354 VDTWEAFKKVELEAVIDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIY-- 413
             TW+  +   ++A+++  L  S             ++++  +      SY +    Y  
Sbjct: 450 KATWKLMQTRGIDAMVNDSLIGSVLSFGASFVAYAAALVAYCFLKYTDPSYNSGGGFYAP 509

Query: 414 ----AFLIGYFLCRIGMAWPQACVSAYYVAYADNPQHPRFDSMVPQRIRELQR 451
               AF+I   +  I     ++  S +++A A +P+  R     PQ   E+ R
Sbjct: 510 VVGLAFVIALQVSNITNVSLKSGCSTFFLALARDPEVLRVS--YPQIYEEICR 560

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BLAST of MC01g0284 vs. NCBI nr
Match: XP_022154493.1 (protein PNS1-like isoform X2 [Momordica charantia])

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0


Query: 1   METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLV 60
           METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLV
Sbjct: 1   METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLV 60

Query: 61  GVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAF 120
           GVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAF
Sbjct: 61  GVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAF 120

Query: 121 WFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEY 180
           WFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEY
Sbjct: 121 WFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEY 180

Query: 181 PPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIV 240
           PPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIV
Sbjct: 181 PPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIV 240

Query: 241 QVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAG 300
           QVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAG
Sbjct: 241 QVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAG 300

Query: 301 ESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVID 360
           ESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVID
Sbjct: 301 ESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVID 360

Query: 361 YDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQAC 420
           YDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQAC
Sbjct: 361 YDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQAC 420

Query: 421 VSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           VSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA
Sbjct: 421 VSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454

[Close]

BLAST of MC01g0284 vs. NCBI nr
Match: XP_022154492.1 (protein PNS1-like isoform X1 [Momordica charantia])

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 454/456 (99.56%), Postives = 454/456 (99.56%), Query Frame = 0


Query: 1   METRTPSVLQNPHQVQERRVPSSTPTK--VQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           METRTPSVLQNPHQVQERRVPSSTPTK  VQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC
Sbjct: 1   METRTPSVLQNPHQVQERRVPSSTPTKLQVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM
Sbjct: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST
Sbjct: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN
Sbjct: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM
Sbjct: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV
Sbjct: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ
Sbjct: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA
Sbjct: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 456

[Close]

BLAST of MC01g0284 vs. NCBI nr
Match: XP_022950675.1 (protein PNS1-like [Cucurbita moschata] >KAG7033642.1 PNS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 697 bits (1799), Expect = 1.29e-249
Identity = 359/456 (78.73%), Postives = 394/456 (86.40%), Query Frame = 0


Query: 1   METRTP-SVLQN-PHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           ME RTP SVLQN  HQ QERRV SSTP +VQEP   P+EP  GWR FRWL S+VF IH+C
Sbjct: 1   MEARTPPSVLQNHSHQAQERRVSSSTPNQVQEPSH-PIEPAMGWRIFRWLSSIVFKIHIC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LV VLVI L V GLRSASKTHHFHP++WYPPLL + GSSGIIAFSWQ FTGC PS AFK 
Sbjct: 61  LVAVLVIVLTVLGLRSASKTHHFHPKEWYPPLLASIGSSGIIAFSWQAFTGCSPSMAFKT 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPV+SLA+G+FFVIVGS GGLAAG ILIVSSL +SVYVCW+  R NYAI+LLS+ST
Sbjct: 121 AFWFSPVLSLASGVFFVIVGSRGGLAAGVILIVSSLIVSVYVCWINHRLNYAIQLLSLST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           +YPPT TPIFV GSIL+G+LYA+ +V GIGGA ++ SS+KA FIAAILLSLTWTLQVIKN
Sbjct: 181 KYPPTNTPIFVFGSILIGVLYAAFVVIGIGGATSITSSFKALFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           +V VTISRIKY NLA G EKD RAAF+D +KH  GTIS+GS I P +SFI GSARAMRL+
Sbjct: 241 VVHVTISRIKYFNLAWGREKDIRAAFYDIIKHSAGTISLGSAIIPFISFIHGSARAMRLV 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AG+SDEFLFSCA C S +AS LRNHGNRWGFVHVGVYNKG+VQAS DTWEAF+KV+LE V
Sbjct: 301 AGDSDEFLFSCAPCYSELASMLRNHGNRWGFVHVGVYNKGIVQASYDTWEAFEKVDLEIV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           ID DLTVSFC LCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYF+CRIGMAWPQ
Sbjct: 361 IDSDLTVSFCVLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFVCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNP HPRFDS VP RIREL+RRLQ 
Sbjct: 421 ACVSAYYVAYADNPLHPRFDSTVPDRIRELRRRLQV 455

[Close]

BLAST of MC01g0284 vs. NCBI nr
Match: XP_023543456.1 (protein PNS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 697 bits (1798), Expect = 1.83e-249
Identity = 358/456 (78.51%), Postives = 394/456 (86.40%), Query Frame = 0


Query: 1   METRTP-SVLQN-PHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           ME RTP SVLQN  HQ QERRV SSTP +VQEP   P+EP  GWR FRWL S+VF IH+C
Sbjct: 1   MEARTPPSVLQNHSHQAQERRVSSSTPNQVQEPSH-PIEPAMGWRIFRWLSSIVFKIHIC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LV VLVI L V GLRSASKTHHFHP++WYPPLL + GSSGIIAFSWQ FTGC PS AFK 
Sbjct: 61  LVAVLVIVLTVLGLRSASKTHHFHPKEWYPPLLASIGSSGIIAFSWQAFTGCSPSMAFKT 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPV+SLA+G+FF+IVGS GGLAAG ILIVSSL +SVYVCW+  R NYAI+LLS+ST
Sbjct: 121 AFWFSPVLSLASGVFFIIVGSRGGLAAGVILIVSSLIVSVYVCWINHRLNYAIQLLSLST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           +YPPT TPIFV GSIL+G+LYA+ +V GIGGA ++ SS+KA FIAAILLSLTWTLQVIKN
Sbjct: 181 KYPPTNTPIFVFGSILIGVLYAAFVVIGIGGATSITSSFKALFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           +V VTISRIKY NLA G EKD RAAF+D +KH  GTIS+GS I P +SFI GSARAMRL+
Sbjct: 241 VVHVTISRIKYFNLAWGREKDIRAAFYDIIKHSAGTISLGSAIIPFISFIHGSARAMRLV 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AG+SDEFLFSCA C S +AS LRNHGNRWGFVHVGVYNKG+VQAS DTWEAF+KV+LE V
Sbjct: 301 AGDSDEFLFSCAPCYSELASMLRNHGNRWGFVHVGVYNKGIVQASYDTWEAFEKVDLEIV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           ID DLTVSFC LCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYF+CRIGMAWPQ
Sbjct: 361 IDSDLTVSFCVLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFVCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNP HPRFDS VP RIREL+RRLQ 
Sbjct: 421 ACVSAYYVAYADNPLHPRFDSTVPDRIRELRRRLQV 455

[Close]

BLAST of MC01g0284 vs. NCBI nr
Match: XP_022978167.1 (protein PNS1-like [Cucurbita maxima])

HSP 1 Score: 690 bits (1780), Expect = 1.00e-246
Identity = 356/456 (78.07%), Postives = 392/456 (85.96%), Query Frame = 0


Query: 1   METRTP-SVLQN-PHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           METRTP SVLQN  HQ QERRV SSTP +VQEP   P EP  GWR FRWL ++VF IH+C
Sbjct: 1   METRTPPSVLQNHSHQAQERRVSSSTPNQVQEPSH-PNEPAVGWRIFRWLSTIVFKIHIC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LV VLVI L V  LRSASKTHHFHP++WYPPLL + GSSGIIAFSWQ FTGC PS AFK 
Sbjct: 61  LVAVLVIVLTVLRLRSASKTHHFHPKEWYPPLLASIGSSGIIAFSWQAFTGCSPSMAFKT 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPV+SLA+G+FFVIVGS GGLAAG ILIVSSL +SVYVCW+  R NYAI+LLS+ST
Sbjct: 121 AFWFSPVLSLASGVFFVIVGSRGGLAAGVILIVSSLIVSVYVCWINHRLNYAIQLLSLST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           +YPPT TPIFV GSI++G+LYA+ +V GIGGA ++ SS+KA FIAAILLSLTWTLQVIKN
Sbjct: 181 KYPPTNTPIFVFGSIVIGVLYAAFMVIGIGGATSITSSFKALFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           +V VTISRIKY NLA G EKD RAAF+D +KH  GTIS+GS I P +SFI GSARAMRL+
Sbjct: 241 VVHVTISRIKYFNLAWGREKDIRAAFYDIIKHSAGTISLGSAIIPFISFIHGSARAMRLV 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AG+SDEFLFSCA C S +AS LRNHGNRWGFVHVGVYNKG+VQAS DTWEAF+KV+LE V
Sbjct: 301 AGDSDEFLFSCAPCYSELASMLRNHGNRWGFVHVGVYNKGIVQASYDTWEAFEKVDLEIV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           ID DLTVSFC LCGVS GAICSIISGIWALVIHKSYATELAIYAFLIGYF+CRIGMAWPQ
Sbjct: 361 IDSDLTVSFCVLCGVSSGAICSIISGIWALVIHKSYATELAIYAFLIGYFVCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNP HPRFDS VP RIREL+RRLQ 
Sbjct: 421 ACVSAYYVAYADNPLHPRFDSTVPDRIRELRRRLQV 455

[Close]

BLAST of MC01g0284 vs. ExPASy TrEMBL
Match: A0A6J1DKG3 (protein PNS1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021760 PE=3 SV=1)

HSP 1 Score: 892 bits (2305), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0


Query: 1   METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLV 60
           METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLV
Sbjct: 1   METRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLV 60

Query: 61  GVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAF 120
           GVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAF
Sbjct: 61  GVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAF 120

Query: 121 WFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEY 180
           WFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEY
Sbjct: 121 WFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVSTEY 180

Query: 181 PPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIV 240
           PPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIV
Sbjct: 181 PPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIV 240

Query: 241 QVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAG 300
           QVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAG
Sbjct: 241 QVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAG 300

Query: 301 ESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVID 360
           ESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVID
Sbjct: 301 ESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAVID 360

Query: 361 YDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQAC 420
           YDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQAC
Sbjct: 361 YDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQAC 420

Query: 421 VSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           VSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA
Sbjct: 421 VSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454

[Close]

BLAST of MC01g0284 vs. ExPASy TrEMBL
Match: A0A6J1DLU3 (protein PNS1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021760 PE=3 SV=1)

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 454/456 (99.56%), Postives = 454/456 (99.56%), Query Frame = 0


Query: 1   METRTPSVLQNPHQVQERRVPSSTPTK--VQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           METRTPSVLQNPHQVQERRVPSSTPTK  VQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC
Sbjct: 1   METRTPSVLQNPHQVQERRVPSSTPTKLQVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM
Sbjct: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST
Sbjct: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN
Sbjct: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM
Sbjct: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV
Sbjct: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ
Sbjct: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA
Sbjct: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 456

[Close]

BLAST of MC01g0284 vs. ExPASy TrEMBL
Match: A0A6J1GGE9 (protein PNS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453700 PE=3 SV=1)

HSP 1 Score: 697 bits (1799), Expect = 6.23e-250
Identity = 359/456 (78.73%), Postives = 394/456 (86.40%), Query Frame = 0


Query: 1   METRTP-SVLQN-PHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           ME RTP SVLQN  HQ QERRV SSTP +VQEP   P+EP  GWR FRWL S+VF IH+C
Sbjct: 1   MEARTPPSVLQNHSHQAQERRVSSSTPNQVQEPSH-PIEPAMGWRIFRWLSSIVFKIHIC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LV VLVI L V GLRSASKTHHFHP++WYPPLL + GSSGIIAFSWQ FTGC PS AFK 
Sbjct: 61  LVAVLVIVLTVLGLRSASKTHHFHPKEWYPPLLASIGSSGIIAFSWQAFTGCSPSMAFKT 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPV+SLA+G+FFVIVGS GGLAAG ILIVSSL +SVYVCW+  R NYAI+LLS+ST
Sbjct: 121 AFWFSPVLSLASGVFFVIVGSRGGLAAGVILIVSSLIVSVYVCWINHRLNYAIQLLSLST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           +YPPT TPIFV GSIL+G+LYA+ +V GIGGA ++ SS+KA FIAAILLSLTWTLQVIKN
Sbjct: 181 KYPPTNTPIFVFGSILIGVLYAAFVVIGIGGATSITSSFKALFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           +V VTISRIKY NLA G EKD RAAF+D +KH  GTIS+GS I P +SFI GSARAMRL+
Sbjct: 241 VVHVTISRIKYFNLAWGREKDIRAAFYDIIKHSAGTISLGSAIIPFISFIHGSARAMRLV 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AG+SDEFLFSCA C S +AS LRNHGNRWGFVHVGVYNKG+VQAS DTWEAF+KV+LE V
Sbjct: 301 AGDSDEFLFSCAPCYSELASMLRNHGNRWGFVHVGVYNKGIVQASYDTWEAFEKVDLEIV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           ID DLTVSFC LCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYF+CRIGMAWPQ
Sbjct: 361 IDSDLTVSFCVLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFVCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNP HPRFDS VP RIREL+RRLQ 
Sbjct: 421 ACVSAYYVAYADNPLHPRFDSTVPDRIRELRRRLQV 455

[Close]

BLAST of MC01g0284 vs. ExPASy TrEMBL
Match: A0A6J1IKE0 (protein PNS1-like OS=Cucurbita maxima OX=3661 GN=LOC111478231 PE=3 SV=1)

HSP 1 Score: 690 bits (1780), Expect = 4.86e-247
Identity = 356/456 (78.07%), Postives = 392/456 (85.96%), Query Frame = 0


Query: 1   METRTP-SVLQN-PHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           METRTP SVLQN  HQ QERRV SSTP +VQEP   P EP  GWR FRWL ++VF IH+C
Sbjct: 1   METRTPPSVLQNHSHQAQERRVSSSTPNQVQEPSH-PNEPAVGWRIFRWLSTIVFKIHIC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LV VLVI L V  LRSASKTHHFHP++WYPPLL + GSSGIIAFSWQ FTGC PS AFK 
Sbjct: 61  LVAVLVIVLTVLRLRSASKTHHFHPKEWYPPLLASIGSSGIIAFSWQAFTGCSPSMAFKT 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPV+SLA+G+FFVIVGS GGLAAG ILIVSSL +SVYVCW+  R NYAI+LLS+ST
Sbjct: 121 AFWFSPVLSLASGVFFVIVGSRGGLAAGVILIVSSLIVSVYVCWINHRLNYAIQLLSLST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           +YPPT TPIFV GSI++G+LYA+ +V GIGGA ++ SS+KA FIAAILLSLTWTLQVIKN
Sbjct: 181 KYPPTNTPIFVFGSIVIGVLYAAFMVIGIGGATSITSSFKALFIAAILLSLTWTLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           +V VTISRIKY NLA G EKD RAAF+D +KH  GTIS+GS I P +SFI GSARAMRL+
Sbjct: 241 VVHVTISRIKYFNLAWGREKDIRAAFYDIIKHSAGTISLGSAIIPFISFIHGSARAMRLV 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AG+SDEFLFSCA C S +AS LRNHGNRWGFVHVGVYNKG+VQAS DTWEAF+KV+LE V
Sbjct: 301 AGDSDEFLFSCAPCYSELASMLRNHGNRWGFVHVGVYNKGIVQASYDTWEAFEKVDLEIV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           ID DLTVSFC LCGVS GAICSIISGIWALVIHKSYATELAIYAFLIGYF+CRIGMAWPQ
Sbjct: 361 IDSDLTVSFCVLCGVSSGAICSIISGIWALVIHKSYATELAIYAFLIGYFVCRIGMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNP HPRFDS VP RIREL+RRLQ 
Sbjct: 421 ACVSAYYVAYADNPLHPRFDSTVPDRIRELRRRLQV 455

[Close]

BLAST of MC01g0284 vs. ExPASy TrEMBL
Match: A0A5D3C054 (Protein PNS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G00220 PE=3 SV=1)

HSP 1 Score: 668 bits (1724), Expect = 1.61e-238
Identity = 347/456 (76.10%), Postives = 386/456 (84.65%), Query Frame = 0


Query: 1   METRTP-SVLQNPH-QVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLC 60
           METR P +VLQN   QVQE RV SSTP KVQEP  L  +P  GWR FRWL SL+F +HLC
Sbjct: 1   METRRPPNVLQNQSLQVQEIRVSSSTPNKVQEPIRLN-KPAVGWRIFRWLSSLIFIVHLC 60

Query: 61  LVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKM 120
           LV VLVIFL V+GL++ASKTHHFHPEKWYPPLL +TGSSGII F WQ FTGC PS A K 
Sbjct: 61  LVAVLVIFLTVRGLQAASKTHHFHPEKWYPPLLASTGSSGIIGFLWQAFTGCSPSMALKT 120

Query: 121 AFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVST 180
           AFWFSPV+SLA+G+FFVIVGS GGLAAG ILIV SL LSVYVCW+  R NYA  LLS+ST
Sbjct: 121 AFWFSPVLSLASGVFFVIVGSRGGLAAGVILIVCSLILSVYVCWINHRLNYATELLSLST 180

Query: 181 EYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKN 240
           +YPP  T IFV GSI++GILYAS LV GIGGA+ALRS + A F+AAILLSL+W+LQVIKN
Sbjct: 181 KYPPKNTSIFVFGSIIIGILYASFLVIGIGGAIALRSGFTALFVAAILLSLSWSLQVIKN 240

Query: 241 IVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLM 300
           IVQVTIS IKYLN A+G EKD RAAFHDT+K+ +GTI +GS I PL+SFIQGSAR+MRL+
Sbjct: 241 IVQVTISCIKYLNFAKGSEKDIRAAFHDTIKNSVGTICLGSAIIPLISFIQGSARSMRLV 300

Query: 301 AGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKVELEAV 360
           AG+SDEFLFSCA+CCSG+AS LR+HGNRWGFVHVGV+NKG+VQAS DTWEAFK+ ELE V
Sbjct: 301 AGDSDEFLFSCANCCSGMASMLRSHGNRWGFVHVGVFNKGIVQASYDTWEAFKRAELEIV 360

Query: 361 IDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQ 420
           I  DLTVSFC LCGVS GAICSIISGIWALVIHK+YATELAI AFLIGYFLCRI MAWPQ
Sbjct: 361 IHSDLTVSFCVLCGVSSGAICSIISGIWALVIHKNYATELAICAFLIGYFLCRIAMAWPQ 420

Query: 421 ACVSAYYVAYADNPQHPRFDSMVPQRIRELQRRLQA 454
           ACVSAYYVAYADNPQ PRF S VP+RI+ LQ R Q 
Sbjct: 421 ACVSAYYVAYADNPQDPRFGSTVPERIQALQWRSQV 455

[Close]

BLAST of MC01g0284 vs. TAIR 10
Match: AT3G03700.1 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.8e-113
Identity = 217/457 (47.48%), Postives = 303/457 (66.30%), Query Frame = 0


Query: 6   PSVLQNPH----QVQERRV-----PSSTPTKVQEPRVLP---VEPTAGWRFFRWLCSLVF 65
           P+   N H    Q+QE R+     PSS+P   +E +  P    + T   + FR L   + 
Sbjct: 2   PTPTSNGHASRVQIQEPRIMSPLPPSSSPIAFKEQQGRPPPTTQQTIAGKLFRTLFKGLL 61

Query: 66  YIHLCLVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCPS 125
           +  L L+ +LVI L ++GL SAS THHFH +KWYPPLL +   SGI + +WQ      PS
Sbjct: 62  FSQLTLISLLVIVLTIRGLISAS-THHFHLKKWYPPLLASVAVSGIASLAWQCIFIYNPS 121

Query: 126 KAFKMAFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRL 185
           +A K  FW SP+++ + GI  V++GSA     GA+ ++ ++  S+Y CW+  R  Y  ++
Sbjct: 122 RAVKATFWLSPILTCSVGILLVLIGSAVDAGIGAVFVLFAITQSLYGCWITPRLEYTDKI 181

Query: 186 LSVSTEYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTL 245
           LS++T +PP +T   V  SI+V ++Y+  LVTGIGGA + R++    FI+ I++SL WT+
Sbjct: 182 LSLATAFPPARTREVVCLSIIVSVVYSGFLVTGIGGATSTRTNLDILFISVIIISLAWTM 241

Query: 246 QVIKNIVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSAR 305
           QVIKN+ QV ISR +Y+N A G + D   AF  TMKHL G+I +GS + P++ FI+GS R
Sbjct: 242 QVIKNVQQVAISRARYVNFAHGEDMDAWNAFRITMKHLTGSICVGSTLVPIIVFIRGSIR 301

Query: 306 AMRLMAGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKV 365
           ++ LM+G SDE ++S A C S IA+ L   GNRWGFVHVG Y+KG ++AS DTW+ F+  
Sbjct: 302 SVNLMSGSSDEVMYSGADCFSTIANKLITLGNRWGFVHVGTYDKGFMEASSDTWKKFRST 361

Query: 366 E-LEAVIDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRI 425
             LE +ID DLT SFCFL  VS GA+ S+ +GIW L+IHK YA E+ +YAF+IGYF+ R+
Sbjct: 362 TGLEKLIDSDLTSSFCFLSAVSVGAVSSLTAGIWMLLIHKDYALEVTLYAFIIGYFVGRV 421

Query: 426 GMAWPQACVSAYYVAYADNPQHPRFDSMVPQRIRELQ 450
           G+AW QACV AYYVAY+++PQ  RFD  +PQRI+ LQ
Sbjct: 422 GLAWLQACVLAYYVAYSEDPQSMRFDGTIPQRIQRLQ 457

[Close]

BLAST of MC01g0284 vs. TAIR 10
Match: AT3G04440.1 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 406.0 bits (1042), Expect = 4.0e-113
Identity = 217/458 (47.38%), Postives = 304/458 (66.38%), Query Frame = 0


Query: 1   METRTPSVLQNPHQVQERRV-----PSSTPTKVQEPRVLP---VEPTAGWRFFRWLCSLV 60
           M T T +   +  Q+QE R+     PSS+P   +E +  P    + T   + FR L   +
Sbjct: 1   MPTPTSNGHASRMQIQEPRIMSPLPPSSSPFAFKEQQGRPPPTTQQTIAGKLFRTLFKGL 60

Query: 61  FYIHLCLVGVLVIFLVVQGLRSASKTHHFHPEKWYPPLLTATGSSGIIAFSWQGFTGCCP 120
            +  L L+ +LVI L ++GL SAS THHFH +KWYPPLL +   SGI + +WQ      P
Sbjct: 61  LFSQLTLISLLVIVLTIRGLISAS-THHFHLKKWYPPLLASVAVSGIASLAWQCIFIYNP 120

Query: 121 SKAFKMAFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIR 180
           S+A K  FW SP+++ + GI  V++GSA     GA+ ++ ++  S+Y CW+  R  Y  +
Sbjct: 121 SRAVKATFWLSPILTCSVGILLVLIGSAVDAGIGAVFVLFAITQSLYGCWITPRLEYTDK 180

Query: 181 LLSVSTEYPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWT 240
           +LS++T +PP +T   V  SI+V ++Y+  LVTGIGGA + R++    FI+ I++SL WT
Sbjct: 181 ILSLATAFPPARTREVVCLSIIVSVVYSGFLVTGIGGATSTRTNLDILFISVIIISLAWT 240

Query: 241 LQVIKNIVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSA 300
           +QVIKN+ QV ISR +Y+N A G + D   AF  TMKHL G+I +GS + P++ FI+GS 
Sbjct: 241 MQVIKNVQQVAISRARYVNFAHGEDMDAWNAFRITMKHLTGSICVGSTLVPIIVFIRGSI 300

Query: 301 RAMRLMAGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKK 360
           R++ LM+G SDE ++S A C S IA+ L   GNRWGFVHVG Y+KG ++AS DTW+ F+ 
Sbjct: 301 RSVNLMSGSSDEVMYSGADCFSTIANKLITLGNRWGFVHVGTYDKGFMEASSDTWKKFRS 360

Query: 361 VE-LEAVIDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCR 420
              LE +ID DLT SFCFL  VS GA+ S+ +GIW L+IHK YA E+ +YAF+IGYF+ R
Sbjct: 361 TTGLEKLIDSDLTSSFCFLSAVSVGAVSSLTAGIWMLLIHKDYALEVTLYAFIIGYFVGR 420

Query: 421 IGMAWPQACVSAYYVAYADNPQHPRFDSMVPQRIRELQ 450
           +G+AW QACV AYYVAY+++PQ  RFD  +PQRI+ LQ
Sbjct: 421 VGLAWLQACVLAYYVAYSEDPQSMRFDGTIPQRIQRLQ 457

[Close]

BLAST of MC01g0284 vs. TAIR 10
Match: AT5G17830.1 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 296.6 bits (758), Expect = 3.4e-80
Identity = 161/426 (37.79%), Postives = 255/426 (59.86%), Query Frame = 0


Query: 24  TPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLVGVLVIFLVVQGLRSASKTHHFHP 83
           TP   Q+ +  P + T   RFFR L + +FY  L L+ + VI L ++GL   +K+ +FHP
Sbjct: 30  TPPAAQQQQQQPQQQTLSGRFFRCLFTCIFYTQLTLISIFVILLTLRGL-VCTKSPNFHP 89

Query: 84  EKWYPPLLTATGSSGIIAFSWQGFTGCCPSKAFKMAFWFSPVVSLAAGIFFVIVGSAGG- 143
           +KWY PLL++   SG+++ +W  F  C      K  FWF+P+ +++ G+F +++  +   
Sbjct: 90  KKWYTPLLSSVAVSGVLSIAWNCFFVCNIRATVKATFWFTPLFTISVGLFLILLDKSNPV 149

Query: 144 -LAAGAILIVSSLFLSVY-VCWVYRRFNYAIRLLSVSTEYPPTKTPIFVLGSILVGILYA 203
            L  GA+L+  S+  +VY    V  R  +  + +S +T   P +T    + S+++ + Y+
Sbjct: 150 VLWIGALLVFYSIVTAVYGSLHVTNRHEFTFQTMSTATGILPARTRAIAVVSVIISVFYS 209

Query: 204 SVLVTGIGGAVALRSSWKAAFIAAILLSLTWTLQVIKNIVQVTISRIKYLNLARGHEKDT 263
             LV GIGGA A  +     FI+ I+++L WT+QVIKN+ +V IS+  Y+  +R    + 
Sbjct: 210 DFLVAGIGGATATGTRLDILFISIIVINLAWTMQVIKNVQEVAISKAIYVYFSRDDLMNA 269

Query: 264 RAAFHDTMKHLIGTISIGSVIAPLVSFIQGSARAMRLMAGESDEFLFSCAHCCSGIASTL 323
             A   T+K  +G++ IGS + PL+   +G+ R         +  +++    C+ IA+ +
Sbjct: 270 CDALGVTLKKQLGSVCIGSTLVPLIVLFRGTIRC-------CNRDIYASTPGCNWIANHI 329

Query: 324 RNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKV-ELEAVIDYDLTVSFCFLCGVSGGAIC 383
              GNR+GFVHVG +NKG+ QAS DTW  F+ + E E +ID+D+T S CF   +  GAI 
Sbjct: 330 ILGGNRYGFVHVGAHNKGLRQASSDTWRRFRTIPEFEQLIDFDITSSICFFSAMGIGAIA 389

Query: 384 SIISGIWALVIHKSYATELAIYAFLIGYFLCRIGMAWPQACVSAYYVAYADNPQHPRFDS 443
           ++ +G+W L+I K +  EL IYAF+IGYF+ R+  AW QACV  YYVAY+++PQ+ RFD 
Sbjct: 390 ALTAGVWELLIDKDHYFELTIYAFIIGYFVGRVSSAWLQACVMGYYVAYSEDPQNDRFDD 447

Query: 444 MVPQRI 446
            +PQRI
Sbjct: 450 TIPQRI 447

[Close]

BLAST of MC01g0284 vs. TAIR 10
Match: AT5G13760.1 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 218.4 bits (555), Expect = 1.2e-56
Identity = 146/448 (32.59%), Postives = 225/448 (50.22%), Query Frame = 0


Query: 2   ETRTPSVLQNPHQVQERRVPSSTPTKVQEPRVLPVEPTAGWRFFRWLCSLVFYIHLCLVG 61
           E   P     P+Q Q R +   TPT  Q    L        +FF  L      + +  VG
Sbjct: 104 EETQPQTPPPPNQHQTRSI--FTPTPQQTLASLNSTKYTN-KFFLLLFIFHKVVAIGFVG 163

Query: 62  VLVIFLVVQGLRSASKTHHFHPEK----WYPPLLTATGSSGIIAFSWQGFTGCCPSKAFK 121
            LV F  VQGL  ++ +     +K      P +  A+  S I+AF WQ      P     
Sbjct: 164 FLV-FRGVQGLIGSNGSVKRKEKKILRFLLPQVEAASLLSIILAFLWQMAFRIWPDFMIH 223

Query: 122 MAFWFSPVVSLAAGIFFVIVGSAGGLAAGAILIVSSLFLSVYVCWVYRRFNYAIRLLSVS 181
              W + ++SL++GI  +        A G  LI  S+   +Y CWV RR  +  ++L  S
Sbjct: 224 FILWSTFLMSLSSGILLLCFQMPATDAVGVCLIAFSIGNGLYACWVTRRIKFCSKILVKS 283

Query: 182 TE----YPPTKTPIFVLGSILVGILYASVLVTGIGGAVALRSSWKAAFIAAILLSLTWTL 241
            E    +     P + +  +  G L+ S+ + G+ G  AL   +    I  ++LSL WT 
Sbjct: 284 LEPVSKFSDLNLPTYYM--LAAGFLWMSMWIFGVIG--ALNFYFPPLVIIGLVLSLAWTT 343

Query: 242 QVIKNIVQVTISRIKYLNLARGHEKDTRAAFHDTMKHLIGTISIGSVIAPLVSFIQGSAR 301
           +V++NIV +T+SR+  L   RG +  TR +F   +   +G+  +GS+  P +  ++  AR
Sbjct: 344 EVMRNIVNLTVSRVIALYYLRGMQSSTRFSFQRALSRNLGSACLGSLFVPTIEALRILAR 403

Query: 302 AMRLMAGESDEFLFSCAHCCSGIASTLRNHGNRWGFVHVGVYNKGMVQASVDTWEAFKKV 361
            + L+ GE DEF+F CA+CC  +   +  HGN W FV +  Y KG V+AS DTW+ F+  
Sbjct: 404 GLNLLKGE-DEFMFCCANCCLRLMDFIFEHGNGWAFVQIAAYGKGFVRASQDTWKLFEDE 463

Query: 362 ELEAVIDYDLTVSFCFLCGVSGGAICSIISGIWALVIHKSYATELAIYAFLIGYFLCRIG 421
           ++  ++D D+T S CFL G+  G +C I++  W   ++K +   +++ AF IGY + RI 
Sbjct: 464 DMVEIVDADITSSICFLTGICSGCVCLIVAAAWTHTVYKPFTATISLLAFFIGYLMTRIS 523

Query: 422 MAWPQACVSAYYVAYADNPQHPRFDSMV 442
           MA P ACV  YY  YA+NP+   F+  V
Sbjct: 524 MALPHACVGCYYTCYAENPESRFFEDKV 542

The following BLAST results are available for this feature:

BLAST of MC01g0284 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4I8E9	4.0e-09	20.67	Protein PNS1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 /...	[more]
Q6C938	1.6e-05	22.32	Protein PNS1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PNS1 ...	[more]

BLAST of MC01g0284 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154493.1	0.0	100.00	protein PNS1-like isoform X2 [Momordica charantia]	[more]
XP_022154492.1	0.0	99.56	protein PNS1-like isoform X1 [Momordica charantia]	[more]
XP_022950675.1	1.29e-249	78.73	protein PNS1-like [Cucurbita moschata] >KAG7033642.1 PNS1, partial [Cucurbita ar...	[more]
XP_023543456.1	1.83e-249	78.51	protein PNS1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022978167.1	1.00e-246	78.07	protein PNS1-like [Cucurbita maxima]	[more]

BLAST of MC01g0284 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DKG3	0.0	100.00	protein PNS1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111021760 PE=3...	[more]
A0A6J1DLU3	0.0	99.56	protein PNS1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021760 PE=3...	[more]
A0A6J1GGE9	6.23e-250	78.73	protein PNS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453700 PE=3 SV=1	[more]
A0A6J1IKE0	4.86e-247	78.07	protein PNS1-like OS=Cucurbita maxima OX=3661 GN=LOC111478231 PE=3 SV=1	[more]
A0A5D3C054	1.61e-238	76.10	Protein PNS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G002...	[more]

BLAST of MC01g0284 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03700.1	1.8e-113	47.48	Plasma-membrane choline transporter family protein	[more]
AT3G04440.1	4.0e-113	47.38	Plasma-membrane choline transporter family protein	[more]
AT5G17830.1	3.4e-80	37.79	Plasma-membrane choline transporter family protein	[more]
AT5G13760.1	1.2e-56	32.59	Plasma-membrane choline transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007603	Choline transporter-like	PFAM	PF04515	Choline_transpo	coord: 148..432 e-value: 7.8E-21 score: 74.7
IPR007603	Choline transporter-like	PANTHER	PTHR12385	CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)	coord: 22..451
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR12385:SF84	F20H23.27 PROTEIN-RELATED	coord: 22..451

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0284.1	MC01g0284.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity