MC01g0246 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0246
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Cysteine proteinase inhibitor
Location	MC01: 9191791 .. 9191931 (-)
RNA-Seq Expression	MC01g0246
Synteny	MC01g0246

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGCTAAAACAGCAGGTAGTTTCCGGTATGTCGTATCATCTTACACTGGAAGCCATCGATGGTGGTAAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTGCAGCCAATGAACTTCCAAGAGTTGCAGGAATTCAACGCATGC

mRNA sequence

AGGCTAAAACAGCAGGTAGTTTCCGGTATGTCGTATCATCTTACACTGGAAGCCATCGATGGTGGTAAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTGCAGCCAATGAACTTCCAAGAGTTGCAGGAATTCAACGCATGC

Coding sequence (CDS)

AGGCTAAAACAGCAGGTAGTTTCCGGTATGTCGTATCATCTTACACTGGAAGCCATCGATGGTGGTAAGAAGAAGGTTTATGAAGCCAAGGTCTGGGTGCAGCCAATGAACTTCCAAGAGTTGCAGGAATTCAACGCATGC

Protein sequence

RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQPMNFQELQEFNAC

Homology

[Close]

BLAST of MC01g0246 vs. ExPASy Swiss-Prot
Match: Q0JNR2 (Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0270100 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 4.2e-10
Identity = 29/43 (67.44%), Postives = 39/43 (90.70%), Query Frame = 0


Query: 3   KQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
           K+QVV+G  +HLTLEA++ G+KKVYEAKVWV+P ++F+ELQEF
Sbjct: 91  KEQVVAGTLHHLTLEALEAGRKKVYEAKVWVKPWLDFKELQEF 133

[Close]

BLAST of MC01g0246 vs. ExPASy Swiss-Prot
Match: Q06445 (Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 7.1e-10
Identity = 31/43 (72.09%), Postives = 37/43 (86.05%), Query Frame = 0


Query: 3  KQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
          +QQVVSG  Y +TLEA DGG+KKVYEAKVW +P +NF+ELQEF
Sbjct: 47 QQQVVSGTLYTITLEAKDGGQKKVYEAKVWEKPWLNFKELQEF 89

[Close]

BLAST of MC01g0246 vs. ExPASy Swiss-Prot
Match: Q8H0X6 (Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 9.3e-10
Identity = 28/45 (62.22%), Postives = 39/45 (86.67%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
           + K+QVV+G  +HLTLE ++ G+KK+YEAKVWV+P +NF+ELQEF
Sbjct: 78  KAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEF 122

[Close]

BLAST of MC01g0246 vs. ExPASy Swiss-Prot
Match: Q10992 (Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.1e-09
Identity = 29/43 (67.44%), Postives = 36/43 (83.72%), Query Frame = 0


Query: 3  KQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQPM-NFQELQEF 45
          K+QVV+G  Y++TLEA +GG KK YEAKVWV+P  NF+ELQEF
Sbjct: 32 KEQVVAGKMYYITLEATNGGVKKTYEAKVWVKPWENFKELQEF 74

[Close]

BLAST of MC01g0246 vs. ExPASy Swiss-Prot
Match: Q8LC76 (Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 2.7e-09
Identity = 28/42 (66.67%), Postives = 36/42 (85.71%), Query Frame = 0


Query: 4   QQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
           +QVV+G  Y LTLE I+ G+KK+YEAKVWV+P MNF++LQEF
Sbjct: 90  EQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEF 131

[Close]

BLAST of MC01g0246 vs. NCBI nr
Match: XP_008455746.1 (PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901819.1 PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >KAA0025834.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa] >TYK09622.1 cysteine proteinase inhibitor A [Cucumis melo var. makuwa])

HSP 1 Score: 76.6 bits (187), Expect = 2.37e-16
Identity = 36/48 (75.00%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVV+G  YHLTLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 121

[Close]

BLAST of MC01g0246 vs. NCBI nr
Match: XP_038880981.1 (cysteine proteinase inhibitor A-like [Benincasa hispida])

HSP 1 Score: 76.3 bits (186), Expect = 6.02e-16
Identity = 35/48 (72.92%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QV++G  YHLTLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 98  KAKEQVIAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 145

[Close]

BLAST of MC01g0246 vs. NCBI nr
Match: XP_022967919.1 (cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967921.1 cysteine proteinase inhibitor A-like [Cucurbita maxima])

HSP 1 Score: 75.5 bits (184), Expect = 6.74e-16
Identity = 36/48 (75.00%), Postives = 41/48 (85.42%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVVSG  YHLTLEAIDGG KKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKPC 121

[Close]

BLAST of MC01g0246 vs. NCBI nr
Match: XP_022932782.1 (cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932784.1 cysteine proteinase inhibitor A-like [Cucurbita moschata] >KAG7034455.1 hypothetical protein SDJN02_04184 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 73.6 bits (179), Expect = 3.86e-15
Identity = 35/48 (72.92%), Postives = 41/48 (85.42%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVV+G  YHLTLEAIDGG KKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKPC 121

[Close]

BLAST of MC01g0246 vs. NCBI nr
Match: KAG6630762.1 (hypothetical protein CIPAW_13G042500 [Carya illinoinensis])

HSP 1 Score: 71.6 bits (174), Expect = 4.35e-15
Identity = 33/44 (75.00%), Postives = 39/44 (88.64%), Query Frame = 0


Query: 3  KQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFN 45
          KQQVVSG  YH+TLEA DGGKKKVYEAK+W +P +NF+ELQ+FN
Sbjct: 10 KQQVVSGTMYHITLEATDGGKKKVYEAKIWEKPWLNFKELQQFN 53

[Close]

BLAST of MC01g0246 vs. ExPASy TrEMBL
Match: A0A1S4E0R5 (Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.15e-16
Identity = 36/48 (75.00%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVV+G  YHLTLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 121

[Close]

BLAST of MC01g0246 vs. ExPASy TrEMBL
Match: A0A5D3CEB5 (Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00160 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.15e-16
Identity = 36/48 (75.00%), Postives = 42/48 (87.50%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVV+G  YHLTLEAIDGGKKKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVAGKLYHLTLEAIDGGKKKVYEAKVWVKPWMNFKQLQEFKLC 121

[Close]

BLAST of MC01g0246 vs. ExPASy TrEMBL
Match: A0A6J1HVR4 (Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.26e-16
Identity = 36/48 (75.00%), Postives = 41/48 (85.42%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVVSG  YHLTLEAIDGG KKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVSGKLYHLTLEAIDGGMKKVYEAKVWVKPWMNFKQLQEFKPC 121

[Close]

BLAST of MC01g0246 vs. ExPASy TrEMBL
Match: A0A6J1F2Q3 (Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.87e-15
Identity = 35/48 (72.92%), Postives = 41/48 (85.42%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEFNAC 47
           + K+QVV+G  YHLTLEAIDGG KKVYEAKVWV+P MNF++LQEF  C
Sbjct: 74  KAKEQVVAGKLYHLTLEAIDGGIKKVYEAKVWVKPWMNFKQLQEFKPC 121

[Close]

BLAST of MC01g0246 vs. ExPASy TrEMBL
Match: A0A059DHH5 (Cysteine proteinase inhibitor (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_A021891 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 9.25e-15
Identity = 32/45 (71.11%), Postives = 40/45 (88.89%), Query Frame = 0


Query: 1  RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 44
          + K+QVV+G  YHLTLEA+D GKKK+YEAKVWV+P MNF++LQEF
Sbjct: 11 KAKEQVVAGTLYHLTLEAVDAGKKKIYEAKVWVKPWMNFKQLQEF 55

[Close]

BLAST of MC01g0246 vs. TAIR 10
Match: AT3G12490.2 (cystatin B )

HSP 1 Score: 63.2 bits (152), Expect = 6.6e-11
Identity = 28/45 (62.22%), Postives = 39/45 (86.67%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
           + K+QVV+G  +HLTLE ++ G+KK+YEAKVWV+P +NF+ELQEF
Sbjct: 78  KAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEF 122

[Close]

BLAST of MC01g0246 vs. TAIR 10
Match: AT3G12490.1 (cystatin B )

HSP 1 Score: 63.2 bits (152), Expect = 6.6e-11
Identity = 28/45 (62.22%), Postives = 39/45 (86.67%), Query Frame = 0


Query: 1  RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
          + K+QVV+G  +HLTLE ++ G+KK+YEAKVWV+P +NF+ELQEF
Sbjct: 45 KAKEQVVAGTLHHLTLEILEAGQKKLYEAKVWVKPWLNFKELQEF 89

[Close]

BLAST of MC01g0246 vs. TAIR 10
Match: AT5G05110.1 (Cystatin/monellin family protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.9e-10
Identity = 28/42 (66.67%), Postives = 36/42 (85.71%), Query Frame = 0


Query: 4   QQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
           +QVV+G  Y LTLE I+ G+KK+YEAKVWV+P MNF++LQEF
Sbjct: 90  EQVVAGKLYRLTLEVIEAGEKKIYEAKVWVKPWMNFKQLQEF 131

[Close]

BLAST of MC01g0246 vs. TAIR 10
Match: AT2G40880.1 (cystatin A )

HSP 1 Score: 58.5 bits (140), Expect = 1.6e-09
Identity = 27/45 (60.00%), Postives = 37/45 (82.22%), Query Frame = 0


Query: 1   RLKQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQP-MNFQELQEF 45
           + ++QVV+G  YHLTLEA +G + K +EAKVWV+P MNF++LQEF
Sbjct: 76  KAREQVVAGTMYHLTLEAKEGDQTKNFEAKVWVKPWMNFKQLQEF 120

[Close]

BLAST of MC01g0246 vs. TAIR 10
Match: AT5G12140.1 (cystatin-1 )

HSP 1 Score: 43.5 bits (101), Expect = 5.4e-05
Identity = 22/44 (50.00%), Postives = 29/44 (65.91%), Query Frame = 0


Query: 3  KQQVVSGMSYHLTLEAIDGGKKKVYEAKVWVQPM-NFQELQEFN 46
          K QVV+G  +HLT+E  DG   KVYEAKV  +   N ++L+ FN
Sbjct: 53 KTQVVAGTMHHLTVEVADGETNKVYEAKVLEKAWENLKQLESFN 96

The following BLAST results are available for this feature:

BLAST of MC01g0246 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0JNR2	4.2e-10	67.44	Cysteine proteinase inhibitor 12 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q06445	7.1e-10	72.09	Cysteine proteinase inhibitor OS=Vigna unguiculata OX=3917 PE=1 SV=1	[more]
Q8H0X6	9.3e-10	62.22	Cysteine proteinase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=CYS6 PE=1 SV=...	[more]
Q10992	2.1e-09	67.44	Cysteine proteinase inhibitor A OS=Helianthus annuus OX=4232 PE=1 SV=1	[more]
Q8LC76	2.7e-09	66.67	Cysteine proteinase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=CYS7 PE=2 SV=...	[more]

BLAST of MC01g0246 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455746.1	2.37e-16	75.00	PREDICTED: cysteine proteinase inhibitor A [Cucumis melo] >XP_016901818.1 PREDIC...	[more]
XP_038880981.1	6.02e-16	72.92	cysteine proteinase inhibitor A-like [Benincasa hispida]	[more]
XP_022967919.1	6.74e-16	75.00	cysteine proteinase inhibitor A-like [Cucurbita maxima] >XP_022967920.1 cysteine...	[more]
XP_022932782.1	3.86e-15	72.92	cysteine proteinase inhibitor A-like [Cucurbita moschata] >XP_022932783.1 cystei...	[more]
KAG6630762.1	4.35e-15	75.00	hypothetical protein CIPAW_13G042500 [Carya illinoinensis]	[more]

BLAST of MC01g0246 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4E0R5	1.15e-16	75.00	Cysteine proteinase inhibitor OS=Cucumis melo OX=3656 GN=LOC103495847 PE=3 SV=1	[more]
A0A5D3CEB5	1.15e-16	75.00	Cysteine proteinase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A6J1HVR4	3.26e-16	75.00	Cysteine proteinase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111467290 PE=3 S...	[more]
A0A6J1F2Q3	1.87e-15	72.92	Cysteine proteinase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111439234 PE=3...	[more]
A0A059DHH5	9.25e-15	71.11	Cysteine proteinase inhibitor (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRS...	[more]

BLAST of MC01g0246 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12490.2	6.6e-11	62.22	cystatin B	[more]
AT3G12490.1	6.6e-11	62.22	cystatin B	[more]
AT5G05110.1	1.9e-10	66.67	Cystatin/monellin family protein	[more]
AT2G40880.1	1.6e-09	60.00	cystatin A	[more]
AT5G12140.1	5.4e-05	50.00	cystatin-1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 3..47 e-value: 8.1E-9 score: 35.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 3..45 e-value: 1.41197E-6 score: 38.9944
None	No IPR available	GENE3D	3.10.450.10		coord: 1..47 e-value: 3.9E-18 score: 67.4
None	No IPR available	PANTHER	PTHR11413:SF110	CYSTEINE PROTEINASE INHIBITOR 7	coord: 3..45
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 3..45
IPR027214	Cystatin	PANTHER	PTHR11413	CYSTATIN FAMILY MEMBER	coord: 3..45

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0246.1	MC01g0246.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006972	hyperosmotic response
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009409	response to cold
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0009414	response to water deprivation
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity