MC01g0230 (gene) Bitter gourd (Dali-11) v1

BLAST of MC01g0230 vs. ExPASy Swiss-Prot
Match: Q5YLB4 (DNA gyrase subunit B, chloroplastic/mitochondrial OS=Nicotiana benthamiana OX=4100 GN=GYRB PE=2 SV=1)

HSP 1 Score: 1141.7 bits (2952), Expect = 0.0e+00
Identity = 583/731 (79.75%), Postives = 645/731 (88.24%), Query Frame = 0

Query: 8   LLLKAPPYFPLRLMASSFLLPSSLHLRSLS-SSASLAPPATFLQLSGTSFRTGKISGIRL 67
           LL   P    LR MAS FLL S  H  +LS SS SL+ P+  L     + +  ++S   L
Sbjct: 3   LLKPFPLQHSLRCMASRFLLHSHYHTHTLSFSSISLSRPSIVLNPRVIN-KLRRLSDAIL 62

Query: 68  VQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLH 127
           ++N+VS +AFMSSS  TE    +   K YGSEQIQ    L+PVRKRPGMYIGSTGPRGLH
Sbjct: 63  IRNMVSLRAFMSSSTTTEAFQENTKSKGYGSEQIQVLEGLDPVRKRPGMYIGSTGPRGLH 122

Query: 128 HLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLETVLTV 187
           HLVYEILDNAVDEAQAGFA++IDVVLHADNSVSI DNGRGIPT+LHP T+KSSLETVLTV
Sbjct: 123 HLVYEILDNAVDEAQAGFATKIDVVLHADNSVSIADNGRGIPTELHPVTKKSSLETVLTV 182

Query: 188 LHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLT 247
           LHAGGKFGGSSSGY+VSGGLHGVGLSVVNALS+ LEV +WRDGKE+ Q YSRGKP+TTL 
Sbjct: 183 LHAGGKFGGSSSGYNVSGGLHGVGLSVVNALSQALEVTIWRDGKEYQQKYSRGKPITTLI 242

Query: 248 CHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLKKEDND 307
           CH    E++ RQGT IRFWPDKEVFTT +QFDYNTI+GRIRELAFLNP+LTI LKKED D
Sbjct: 243 CHDLPVEMRDRQGTAIRFWPDKEVFTTEMQFDYNTIAGRIRELAFLNPELTIALKKEDID 302

Query: 308 LENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADAYSDTI 367
            E  Q NEY YAGGLVEYV WLN+DKKPLH DV GFRKE DG+ ID+ALQWC+DAYSDT+
Sbjct: 303 PEKIQCNEYFYAGGLVEYVKWLNADKKPLH-DVLGFRKEADGITIDMALQWCSDAYSDTM 362

Query: 368 LGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVR 427
           LGYANSIRTIDGGTHIDG+KA+LTR LNNLGKKSK IK+KDISLSGEHVREGLTCV+SV+
Sbjct: 363 LGYANSIRTIDGGTHIDGVKAALTRILNNLGKKSKTIKEKDISLSGEHVREGLTCVISVK 422

Query: 428 VQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKAALAAK 487
           V NPEFEGQTKTRLGNPEVRKVV+QS+QEYLTEYLELHP+VLD ILSKSLNALKAALAAK
Sbjct: 423 VPNPEFEGQTKTRLGNPEVRKVVDQSVQEYLTEYLELHPDVLDSILSKSLNALKAALAAK 482

Query: 488 RARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRFQAILPL 547
           RARELVR+K+VL+SSSLPGKLADC++TNPEE+EIFIVEGDSAGGSAKQGRDRRFQAILPL
Sbjct: 483 RARELVRQKSVLKSSSLPGKLADCSATNPEEAEIFIVEGDSAGGSAKQGRDRRFQAILPL 542

Query: 548 RGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDADVDGAHI 607
           RGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDF+KE LRYHK+IILTDADVDGAHI
Sbjct: 543 RGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFKKEALRYHKIIILTDADVDGAHI 602

Query: 608 RTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFPQNASYN 667
           RTLLLTFFFRYQ+AL++EGCIY+GVPPLYKVERGKQVYYCYDD ELK +Q +FP NASYN
Sbjct: 603 RTLLLTFFFRYQRALFEEGCIYVGVPPLYKVERGKQVYYCYDDAELKKVQRSFPSNASYN 662

Query: 668 IQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRKELIQKS 727
           IQRFKGLGEMMPAQLWETTM+PE R+LKQL VEDAAEANVVFSSLMG+RVD RK+LIQ S
Sbjct: 663 IQRFKGLGEMMPAQLWETTMNPETRLLKQLVVEDAAEANVVFSSLMGSRVDIRKQLIQNS 722

Query: 728 ANLINIGHLDI 736
           A+++N+  LDI
Sbjct: 723 ASMMNLEQLDI 731

BLAST of MC01g0230 vs. ExPASy Swiss-Prot
Match: Q9SS38 (DNA gyrase subunit B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GYRBC PE=2 SV=3)

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 558/727 (76.75%), Postives = 624/727 (85.83%), Query Frame = 0

Query: 19  RLMAS-------SFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKISGIRLVQ-NV 78
           RLMAS       S  L  SLH  S + S+S              F+   +   RLVQ N 
Sbjct: 16  RLMASRPRPRLFSHSLYPSLHRHSSALSSS---------TPRIKFQLANVFSQRLVQRNA 75

Query: 79  VSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLHHLVY 138
           VS K+FMSS+   E+   S + K Y SE IQ    L+PVRKRPGMYIGSTG RGLHHLVY
Sbjct: 76  VSPKSFMSST--MESLQESSTSKDYSSEHIQVLEGLDPVRKRPGMYIGSTGSRGLHHLVY 135

Query: 139 EILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLETVLTVLHAG 198
           EILDNA+DEAQAGFAS+IDVVLH+D+SVSI+DNGRGIPTDLHPAT KSSLETVLTVLHAG
Sbjct: 136 EILDNAIDEAQAGFASKIDVVLHSDDSVSISDNGRGIPTDLHPATGKSSLETVLTVLHAG 195

Query: 199 GKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLTCHVP 258
           GKFGG SSGYSVSGGLHGVGLSVVNALSE LEV V RDG EF Q YSRGKPVTTLTCHV 
Sbjct: 196 GKFGGKSSGYSVSGGLHGVGLSVVNALSEALEVIVRRDGMEFQQKYSRGKPVTTLTCHVL 255

Query: 259 SSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLKKEDNDLENN 318
             E +  QGT IRFWPDKEVFTT IQFD+NTI+GRIRELAFLNPK+TI+LKKED+D E +
Sbjct: 256 PPESRGTQGTCIRFWPDKEVFTTAIQFDHNTIAGRIRELAFLNPKVTISLKKEDDDPERD 315

Query: 319 QYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADAYSDTILGYA 378
            Y+EY YAGGL EYV+WLN+DKKPLH DV GFRKE +G  +DV+LQWC+DAYSDT+LGYA
Sbjct: 316 VYSEYFYAGGLTEYVSWLNTDKKPLH-DVLGFRKEINGSTVDVSLQWCSDAYSDTMLGYA 375

Query: 379 NSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVRVQNP 438
           NSIRTIDGGTHI+G+KASLTRTLN+L KK K+IK+KDISLSGEHVREGLTC+VSV+V NP
Sbjct: 376 NSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKEKDISLSGEHVREGLTCIVSVKVPNP 435

Query: 439 EFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKAALAAKRARE 498
           EFEGQTKTRLGNPEVRK+V+QS+QEYLTEYLELHP+VL+ I+SKSLNA KAALAAKRARE
Sbjct: 436 EFEGQTKTRLGNPEVRKIVDQSVQEYLTEYLELHPDVLESIISKSLNAYKAALAAKRARE 495

Query: 499 LVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKI 558
           LVR K+VL+SSSLPGKLADC+ST+P ESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKI
Sbjct: 496 LVRSKSVLKSSSLPGKLADCSSTDPAESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKI 555

Query: 559 LNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDADVDGAHIRTLL 618
           LNIERKDEAAMYKNEEIQNLILGLGLGVKGEDF KE+LRYHK+IILTDADVDGAHIRTLL
Sbjct: 556 LNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFNKENLRYHKIIILTDADVDGAHIRTLL 615

Query: 619 LTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFPQNASYNIQRF 678
           LTFFFRYQ+AL+D GCIY+GVPPL+KVERGKQ +YCYDD  LK + ++FP NASYNIQRF
Sbjct: 616 LTFFFRYQRALFDAGCIYVGVPPLFKVERGKQAHYCYDDAALKKITASFPGNASYNIQRF 675

Query: 679 KGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRKELIQKSANLI 736
           KGLGEMMPAQLWETTM+P+ R+LKQL V+DAAE N+VFSSLMGARVD RKELI+ +A  +
Sbjct: 676 KGLGEMMPAQLWETTMNPDTRILKQLVVDDAAETNMVFSSLMGARVDVRKELIKSAATRM 730

BLAST of MC01g0230 vs. ExPASy Swiss-Prot
Match: Q94BZ7 (DNA gyrase subunit B, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GYRBM PE=2 SV=1)

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 543/719 (75.52%), Postives = 614/719 (85.40%), Query Frame = 0

Query: 19  RLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKISGIRLVQNVVSAKAFMS 78
           RL +SS       H  +LSS    +P  +F       F+   +   RL+Q    +  F+S
Sbjct: 23  RLFSSSLSPALHRHSSTLSSPPFSSPSPSF----RLKFQLTSVLSQRLIQRNAISSRFLS 82

Query: 79  SSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLHHLVYEILDNAVD 138
           + A+ ET T     K Y SEQIQ    L+PVRKRPGMYIGSTG RGLHHLVYEILDNA+D
Sbjct: 83  TEASQETTTS----KGYSSEQIQVLEGLDPVRKRPGMYIGSTGSRGLHHLVYEILDNAID 142

Query: 139 EAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLETVLTVLHAGGKFGGSSS 198
           EAQAG+AS++DVVLHAD SVS+ DNGRGIPTDLHPAT+KSSLETVLTVLHAGGKFGG+SS
Sbjct: 143 EAQAGYASKVDVVLHADGSVSVVDNGRGIPTDLHPATKKSSLETVLTVLHAGGKFGGTSS 202

Query: 199 GYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLTCHVPSSELKHRQ 258
           GYSVSGGLHGVGLSVVNALSE LEV VWRDG E  QNYSRGKP+TTLTC V   E K  +
Sbjct: 203 GYSVSGGLHGVGLSVVNALSEALEVSVWRDGMEHKQNYSRGKPITTLTCRVLPLESKGTK 262

Query: 259 GTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLKKEDNDLENNQYNEYCYA 318
           GT IRFWPDKEVFTT I+FD+NTI+GRIRELAFLNPK+TI+LKKED+D E  QY+EY +A
Sbjct: 263 GTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTISLKKEDDDPEKTQYSEYSFA 322

Query: 319 GGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDG 378
           GGL EYV+WLN+DK P+H DV GFR+E +G  +DVALQWC+DAYSDT+LGYANSIRTIDG
Sbjct: 323 GGLTEYVSWLNTDKNPIH-DVLGFRREINGATVDVALQWCSDAYSDTMLGYANSIRTIDG 382

Query: 379 GTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKT 438
           GTHI+G+KASLTRTLN L KKSK +K+KDISLSGEHVREGLTC+VSV+V NPEFEGQTKT
Sbjct: 383 GTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKT 442

Query: 439 RLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKAALAAKRARELVRKKNVL 498
           RLGNPEVRK+V+QS+QEYLTE+LELHP++L+ I+SKSLNA KAALAAKRARELVR K+VL
Sbjct: 443 RLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKSLNAYKAALAAKRARELVRSKSVL 502

Query: 499 RSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDE 558
           +SSSLPGKLADC+ST+PE SEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDE
Sbjct: 503 KSSSLPGKLADCSSTDPEVSEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDE 562

Query: 559 AAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDADVDGAHIRTLLLTFFFRYQ 618
           AAMYKNEEIQNLILGLGLGVKGEDF+KE+LRYHK+IILTDADVDGAHIRTLLLTFFFRYQ
Sbjct: 563 AAMYKNEEIQNLILGLGLGVKGEDFKKENLRYHKIIILTDADVDGAHIRTLLLTFFFRYQ 622

Query: 619 KALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFPQNASYNIQRFKGLGEMMP 678
           +AL+D GCIY+GVPPL+KVERGK   YCYDD +LK + S FP NASYNIQRFKGLGEMMP
Sbjct: 623 RALFDAGCIYVGVPPLFKVERGKNAQYCYDDADLKKITSNFPANASYNIQRFKGLGEMMP 682

Query: 679 AQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRKELIQKSANLINIGHLDI 736
            QLWETTM+PE R+LKQL V+D AEAN+ FSSLMGARVD RKELI+ +A  IN+  LDI
Sbjct: 683 EQLWETTMNPETRILKQLVVDDIAEANMTFSSLMGARVDVRKELIKNAATRINLQRLDI 732

BLAST of MC01g0230 vs. ExPASy Swiss-Prot
Match: Q5NBJ3 (DNA gyrase subunit B, chloroplastic/mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GYRB PE=2 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 3.6e-310
Identity = 537/736 (72.96%), Postives = 622/736 (84.51%), Query Frame = 0

Query: 8   LLLKAPPYFPLRLMASSFLLPSS------LHLRSLSSSASLAPPATFLQLSGTSFRTGKI 67
           LL  +PP   LRL+    L  ++      L L + SS+  L  P   +  +         
Sbjct: 4   LLRSSPPPRHLRLLLRRLLSTAAGRPSRLLPLPASSSARLLVRPRVAVAAAA-------- 63

Query: 68  SGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTG 127
           +G  L +N V+ +AFM+S+AA+E          Y +  +Q   +L+ VR RPGMYIGSTG
Sbjct: 64  AGAPLRRNGVAVRAFMASTAASEAMQEKRVAGEYTAANVQVLEALDGVRTRPGMYIGSTG 123

Query: 128 PRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLE 187
            RGLHHLVYEILDNAVDEAQAG+A+++DV+LH DNSVS+TDNGRGIPTD+HP T+KS +E
Sbjct: 124 SRGLHHLVYEILDNAVDEAQAGYATKVDVILHGDNSVSVTDNGRGIPTDIHPQTKKSCVE 183

Query: 188 TVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKP 247
           TVLT++HAGGKFGGS SGY+VSGGLHGVGLSVVNALSE LEV VWRDGKE+ QNYSRGK 
Sbjct: 184 TVLTLMHAGGKFGGSKSGYTVSGGLHGVGLSVVNALSEALEVTVWRDGKEYRQNYSRGKA 243

Query: 248 VTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLK 307
           +T LT    S E   RQGTRIRFWPDK +FTT + FD+NTI+GRIRELAFLNP+LTI L 
Sbjct: 244 ITMLTSRTLSDESSSRQGTRIRFWPDKHIFTTTMDFDFNTIAGRIRELAFLNPELTIALT 303

Query: 308 KEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADA 367
           KE++DL+  Q+NEYCYAGGLVEYV WLN+DKK LHD +  FRKE DG+ +DV+LQWC+D+
Sbjct: 304 KEEDDLQ-VQHNEYCYAGGLVEYVKWLNTDKKSLHDPI-AFRKEMDGITVDVSLQWCSDS 363

Query: 368 YSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTC 427
           YSDT+LGYANSIRTIDGGTHIDG+K SLTRT+NN  KKSK +KDKDISLSGEHVREG+TC
Sbjct: 364 YSDTVLGYANSIRTIDGGTHIDGLKTSLTRTINNFAKKSKTLKDKDISLSGEHVREGMTC 423

Query: 428 VVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKA 487
           +++V+V NPEFEGQTKTRLGNPEVR++VEQS+QE LTEYLELHP+VLD ILSKSLNALKA
Sbjct: 424 IIAVKVPNPEFEGQTKTRLGNPEVRRIVEQSVQENLTEYLELHPDVLDSILSKSLNALKA 483

Query: 488 ALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRFQ 547
           ALAAKRARELVR K+VL+SSSLPGKLADCAS++PEESEIFIVEGDSAGGSAKQGRDR+FQ
Sbjct: 484 ALAAKRARELVRTKSVLKSSSLPGKLADCASSDPEESEIFIVEGDSAGGSAKQGRDRKFQ 543

Query: 548 AILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDADV 607
           AILPLRGKILNIER+DEAA+YKNEEIQNLI+ LGLGVKGEDF KE LRYHK++ILTDADV
Sbjct: 544 AILPLRGKILNIERRDEAALYKNEEIQNLIVALGLGVKGEDFNKEALRYHKIVILTDADV 603

Query: 608 DGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFPQ 667
           DGAHIRTLLLTFFFRYQKAL+DEGCIY+GVPPLYKVERGKQ +YCYDD +LK L +TFP 
Sbjct: 604 DGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLYKVERGKQAHYCYDDADLKELVNTFPT 663

Query: 668 NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRKE 727
           NASY+IQRFKGLGEMMPAQLWETTMDPE+RMLKQL VEDAAEANVVFSSLMG RVD RK+
Sbjct: 664 NASYHIQRFKGLGEMMPAQLWETTMDPERRMLKQLKVEDAAEANVVFSSLMGTRVDVRKQ 723

Query: 728 LIQKSANLINIGHLDI 736
           LIQ +A+++N+ HLDI
Sbjct: 724 LIQNAASMVNLEHLDI 729

BLAST of MC01g0230 vs. ExPASy Swiss-Prot
Match: O50627 (DNA gyrase subunit B OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=gyrB PE=3 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.8e-184
Identity = 334/645 (51.78%), Postives = 447/645 (69.30%), Query Frame = 0

Query: 93  KAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVL 152
           +AY   QIQ    LE VRKRPGMYIGST  RGLHHLV+EI+DN++DEA AGF  +I VV+
Sbjct: 6   QAYDESQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVWEIVDNSIDEAMAGFCDEIGVVI 65

Query: 153 HADNSVSITDNGRGIPTDLHPATRKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLS 212
             DNS+++TDNGRGIP  +H    + ++E ++TVLHAGGKFGG   GY VSGGLHGVG S
Sbjct: 66  EEDNSITVTDNGRGIPVGIHEKMGRPAVEVIMTVLHAGGKFGG--GGYKVSGGLHGVGAS 125

Query: 213 VVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFT 272
           VVNALS +LEV V R+GK  +Q + RG P   L   + +++   R GT+I F PD ++FT
Sbjct: 126 VVNALSTMLEVEVHREGKVHYQKFHRGVPAADLEV-IGTTD---RTGTKIHFKPDGDIFT 185

Query: 273 TVIQFDYNTISGRIRELAFLNPKLTITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDK 332
               F+Y+T++ R+RELAFLN  L I   K  +  E  + +E+ Y GG+  +V  LN  +
Sbjct: 186 ETTVFEYDTLASRLRELAFLNKGLRI---KITDMREEEKSDEFHYEGGIASFVEHLNRTR 245

Query: 333 KPLHDDVFGFRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRT 392
           + LH+       E  GV +++A+Q+  D ++  I  +AN+I T +GGTH  G K  LTR 
Sbjct: 246 ETLHETPIHIEGEKQGVYVEIAVQY-NDGFTSNIYSFANNINTHEGGTHESGFKTGLTRV 305

Query: 393 LNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQS 452
           +N+  +K  + K+ D +L+GE VREGLT ++SV++ +P+FEGQTKT+LGN E R + +  
Sbjct: 306 INDYARKHNLFKESDPNLTGEDVREGLTAIISVKIPDPQFEGQTKTKLGNSEARTITDSL 365

Query: 453 IQEYLTEYLELHPNVLDLILSKSLNALKAALAAKRARELVRKKNVLRSSSLPGKLADCAS 512
             E+ + +L  HP V   ++ K L A +A  AAK+AREL R+K+ L  SSLPGKLADC+S
Sbjct: 366 FTEHFSRFLIEHPQVARKLVDKGLMASRAREAAKKARELTRRKSALEVSSLPGKLADCSS 425

Query: 513 TNPEESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLIL 572
            +   SEI+IVEGDSAGGSAKQGRDR FQAILPLRGKILN+E+     +  N EI+ +I 
Sbjct: 426 RDASISEIYIVEGDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDKILANNEIRAIIT 485

Query: 573 GLGLGVKGEDFRKEDLRYHKVIILTDADVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVP 632
            LG G+ G+DF  E  RYHK+II+TDADVDGAHIRTL+LTFF+RY + L + G +YI  P
Sbjct: 486 ALGTGI-GDDFDIEKARYHKIIIMTDADVDGAHIRTLILTFFYRYMRPLIEHGYVYIAQP 545

Query: 633 PLYKVERGKQVYYCYDDIELKNLQSTFPQNASYNIQRFKGLGEMMPAQLWETTMDPEQRM 692
           PLYKV++GK + Y Y D EL+ + S  P  A   IQR+KGLGEM P+QLWETTMDP QR 
Sbjct: 546 PLYKVQQGKDIQYAYGDAELQEILSQLPDKAKPGIQRYKGLGEMNPSQLWETTMDPGQRT 605

Query: 693 LKQLTVEDAAEANVVFSSLMGARVDFRKELIQKSANLINIGHLDI 736
           + Q+T+EDA +A+ +F  LMG RV+ R++ IQ++A  +   +LDI
Sbjct: 606 VLQVTLEDAMKADEIFEILMGDRVEPRRDFIQENARYVK--NLDI 637

BLAST of MC01g0230 vs. NCBI nr
Match: XP_022153898.1 (DNA gyrase subunit B, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1437 bits (3721), Expect = 0.0
Identity = 733/737 (99.46%), Postives = 733/737 (99.46%), Query Frame = 0

Query: 1   MALPLRHLLLKAPPYFPLRLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGK 60
           MALPLRHLLLKAPPYFPLRLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGK
Sbjct: 1   MALPLRHLLLKAPPYFPLRLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGK 60

Query: 61  ISGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGST 120
           ISGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQ    LEPVRKRPGMYIGST
Sbjct: 61  ISGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQVLEGLEPVRKRPGMYIGST 120

Query: 121 GPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSL 180
           GPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSL
Sbjct: 121 GPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSL 180

Query: 181 ETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGK 240
           ETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGK
Sbjct: 181 ETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGK 240

Query: 241 PVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL 300
           PVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL
Sbjct: 241 PVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL 300

Query: 301 KKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCAD 360
           KKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCAD
Sbjct: 301 KKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCAD 360

Query: 361 AYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLT 420
           AYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLT
Sbjct: 361 AYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLT 420

Query: 421 CVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALK 480
           CVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALK
Sbjct: 421 CVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALK 480

Query: 481 AALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRF 540
           AALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRF
Sbjct: 481 AALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRF 540

Query: 541 QAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDAD 600
           QAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDAD
Sbjct: 541 QAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDAD 600

Query: 601 VDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFP 660
           VDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFP
Sbjct: 601 VDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFP 660

Query: 661 QNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRK 720
           QNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRK
Sbjct: 661 QNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRK 720

Query: 721 ELIQKSANLINIGHLDI 735
           ELIQKSANLINIGHLDI
Sbjct: 721 ELIQKSANLINIGHLDI 737

BLAST of MC01g0230 vs. NCBI nr
Match: XP_023544528.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1293 bits (3347), Expect = 0.0
Identity = 666/740 (90.00%), Postives = 696/740 (94.05%), Query Frame = 0

Query: 1   MALPLRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTG 60
           MAL    LLLK PPY  LRLMASS  L PSS+HLRSL+S+AS AP  +FL+ SGT FR  
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KI-SGIRLVQNVV-SAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYI 120
           KI + +RL QNVV SAKA MSSSAAT+T  GSESLKAYGSEQIQ    L+PVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSSAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHPAT+K
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPATKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEVC+WRDGKEFHQ YS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKP TTLTCHVPS+ELK  QGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQW 360
           ITL KED+D+E NQYNEYCYAGGLVEYVAWLNSDKKPLHD VFGFRKETDGVAID+ALQW
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHD-VFGFRKETDGVAIDIALQW 360

Query: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE 420
           CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE
Sbjct: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE 420

Query: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLN 480
           GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHP+VLDLILSKSLN
Sbjct: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN 480

Query: 481 ALKAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRD 540
           ALKAALAAKRARELVR+K+VLRSSSLPGKLADC+ST+PE+SEIFIVEGDSAGGSAKQGRD
Sbjct: 481 ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRD 540

Query: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILT 600
           RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF+KE LRYHK+IILT
Sbjct: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT 600

Query: 601 DADVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQS 660
           DADVDGAHIRTLLLTFFFRYQKAL+DEGCIYIGVPPLYKVERGKQ YYCYDD ELK LQS
Sbjct: 601 DADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKQAYYCYDDKELKRLQS 660

Query: 661 TFPQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVD 720
           +FP NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQL +EDAAEANVVFSSLMG RVD
Sbjct: 661 SFPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGVRVD 720

Query: 721 FRKELIQKSANLINIGHLDI 735
           FRKELIQKSAN+I++GHLDI
Sbjct: 721 FRKELIQKSANMIDLGHLDI 739

BLAST of MC01g0230 vs. NCBI nr
Match: XP_038882822.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Benincasa hispida])

HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 673/736 (91.44%), Postives = 691/736 (93.89%), Query Frame = 0

Query: 5   LRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKIS- 64
           LRHLL KAP Y PLRLMASS  L PSS+HLRSLSSSASLAP  TFLQ SGT FRT KIS 
Sbjct: 3   LRHLL-KAPGYLPLRLMASSTSLFPSSIHLRSLSSSASLAPSFTFLQSSGTRFRTRKIST 62

Query: 65  GIRLVQNVVSAKAFMSSSAAT-ETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTG 124
            IRLVQNV    AFMSSSAAT ET T SESLKAYGSEQIQ    L+PVRKRPGMYIGSTG
Sbjct: 63  SIRLVQNV----AFMSSSAATTETSTASESLKAYGSEQIQVLEGLDPVRKRPGMYIGSTG 122

Query: 125 PRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLE 184
           PRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHP T+KSSLE
Sbjct: 123 PRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPVTKKSSLE 182

Query: 185 TVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKP 244
           TVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEV VWRDGKEFHQ YSRGKP
Sbjct: 183 TVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVSVWRDGKEFHQKYSRGKP 242

Query: 245 VTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLK 304
            T L CHVPSSELK+ QGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL 
Sbjct: 243 ATILACHVPSSELKNHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLT 302

Query: 305 KEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADA 364
           KEDNDL+NNQYNEYCYAGGLVEYVAWLNSDKKPLHD VF FRKETDGVAID ALQWCADA
Sbjct: 303 KEDNDLKNNQYNEYCYAGGLVEYVAWLNSDKKPLHD-VFAFRKETDGVAIDAALQWCADA 362

Query: 365 YSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTC 424
           YSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKI KDKDISLSGEHVREGLTC
Sbjct: 363 YSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIFKDKDISLSGEHVREGLTC 422

Query: 425 VVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKA 484
           VVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHP+VLDLILSKSLNALKA
Sbjct: 423 VVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNALKA 482

Query: 485 ALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRFQ 544
           ALAAKRARELVR+K+VLRSSSLPGKLADC+STNPEESEIFIVEGDSAGGSAKQGRDRRFQ
Sbjct: 483 ALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEESEIFIVEGDSAGGSAKQGRDRRFQ 542

Query: 545 AILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDADV 604
           AILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF+KE LRYHK+IILTDADV
Sbjct: 543 AILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDADV 602

Query: 605 DGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFPQ 664
           DGAHIRTLLLTFFFRYQKAL+DEGCIYIGVPPLYKVERGKQV+YCYDD ELK LQS+FP 
Sbjct: 603 DGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKQVHYCYDDKELKKLQSSFPS 662

Query: 665 NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRKE 724
           NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQL +E+ A+ANVVFSSLMGARVDFRKE
Sbjct: 663 NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEEEADANVVFSSLMGARVDFRKE 722

Query: 725 LIQKSANLINIGHLDI 735
           LIQKSAN+IN  HLDI
Sbjct: 723 LIQKSANMINFEHLDI 732

BLAST of MC01g0230 vs. NCBI nr
Match: XP_022932851.1 (DNA gyrase subunit B, chloroplastic/mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1293 bits (3345), Expect = 0.0
Identity = 665/740 (89.86%), Postives = 697/740 (94.19%), Query Frame = 0

Query: 1   MALPLRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTG 60
           MAL    LLLK PPY  LRLMASS  L PSS+HLRSL+S+AS AP  +FL+ SGT FR  
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KI-SGIRLVQNVV-SAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYI 120
           KI + +RL QNVV SAKA MSSSAAT+T  GSESLKAYGSEQIQ    L+PVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSSAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHPAT+K
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPATKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEVC+WRDGKEFHQ YS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKP TTLTCHVPS+ELK  QGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQW 360
           ITL KED+D+E NQYNEYCYAGGLVEYVAWLNSDKKPLHD VFGFRKETDGVAID+ALQW
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHD-VFGFRKETDGVAIDIALQW 360

Query: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE 420
           CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGK+SKIIKDKDISLSGEHVRE
Sbjct: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKRSKIIKDKDISLSGEHVRE 420

Query: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLN 480
           GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHP+VLDLILSKSLN
Sbjct: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN 480

Query: 481 ALKAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRD 540
           ALKAALAAKRARELVR+K+VLRSSSLPGKLADC+ST+PE+SEIFIVEGDSAGGSAKQGRD
Sbjct: 481 ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRD 540

Query: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILT 600
           RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF+KE LRYHK+IILT
Sbjct: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT 600

Query: 601 DADVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQS 660
           DADVDGAHIRTLLLTFFFRYQKAL+D+GCIYIGVPPLYKVERGKQ YYCYDD ELK LQS
Sbjct: 601 DADVDGAHIRTLLLTFFFRYQKALFDQGCIYIGVPPLYKVERGKQAYYCYDDKELKRLQS 660

Query: 661 TFPQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVD 720
           +FP NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQL +EDAAEANVVFSSLMGARVD
Sbjct: 661 SFPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGARVD 720

Query: 721 FRKELIQKSANLINIGHLDI 735
           FRKELIQKSAN+I++GHLDI
Sbjct: 721 FRKELIQKSANMIDLGHLDI 739

BLAST of MC01g0230 vs. NCBI nr
Match: KAG6603309.1 (DNA gyrase subunit B, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1290 bits (3339), Expect = 0.0
Identity = 663/740 (89.59%), Postives = 696/740 (94.05%), Query Frame = 0

Query: 1   MALPLRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTG 60
           MAL    LLLK PPY  LRLMASS  L PSS+HLRSL+S+AS AP  +FL+ SGT FR  
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KI-SGIRLVQNVV-SAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYI 120
           KI + +RL QNVV SAKA MSS+AAT+T  GSESLKAYGSEQIQ    L+PVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSTAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHP T+K
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEVC+WRDGKEFHQ YS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKP TTLTCHVPS+ELK  QGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQW 360
           ITL KED+D+E NQYNEYCYAGGLVEYVAWLNSDKKPLHD VFGFRKETDGVAID+ALQW
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHD-VFGFRKETDGVAIDIALQW 360

Query: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE 420
           CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGK+SKIIKDKDISLSGEHVRE
Sbjct: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKRSKIIKDKDISLSGEHVRE 420

Query: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLN 480
           GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHP+VLDLILSKSLN
Sbjct: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN 480

Query: 481 ALKAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRD 540
           ALKAALAAKRARELVR+K+VLRSSSLPGKLADC+ST+PE+SEIFIVEGDSAGGSAKQGRD
Sbjct: 481 ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRD 540

Query: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILT 600
           RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF+KE LRYHK+IILT
Sbjct: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT 600

Query: 601 DADVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQS 660
           DADVDGAHIRTLLLTFFFRYQKAL+DEGCIYIGVPPLYKVERGKQ YYCYD+ ELK LQS
Sbjct: 601 DADVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKQAYYCYDEKELKRLQS 660

Query: 661 TFPQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVD 720
           +FP NA+YNIQRFKGLGEMMPAQLWETTMDPEQRMLKQL +EDAAEANVVFSSLMGARVD
Sbjct: 661 SFPSNATYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGARVD 720

Query: 721 FRKELIQKSANLINIGHLDI 735
           FRKELIQKSAN+IN+GHLDI
Sbjct: 721 FRKELIQKSANMINLGHLDI 739

BLAST of MC01g0230 vs. ExPASy TrEMBL
Match: A0A6J1DIS6 (DNA gyrase subunit B OS=Momordica charantia OX=3673 GN=LOC111021304 PE=3 SV=1)

HSP 1 Score: 1437 bits (3721), Expect = 0.0
Identity = 733/737 (99.46%), Postives = 733/737 (99.46%), Query Frame = 0

Query: 1   MALPLRHLLLKAPPYFPLRLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGK 60
           MALPLRHLLLKAPPYFPLRLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGK
Sbjct: 1   MALPLRHLLLKAPPYFPLRLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGK 60

Query: 61  ISGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGST 120
           ISGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQ    LEPVRKRPGMYIGST
Sbjct: 61  ISGIRLVQNVVSAKAFMSSSAATETPTGSESLKAYGSEQIQVLEGLEPVRKRPGMYIGST 120

Query: 121 GPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSL 180
           GPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSL
Sbjct: 121 GPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSL 180

Query: 181 ETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGK 240
           ETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGK
Sbjct: 181 ETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGK 240

Query: 241 PVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL 300
           PVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL
Sbjct: 241 PVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITL 300

Query: 301 KKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCAD 360
           KKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCAD
Sbjct: 301 KKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCAD 360

Query: 361 AYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLT 420
           AYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLT
Sbjct: 361 AYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLT 420

Query: 421 CVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALK 480
           CVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALK
Sbjct: 421 CVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALK 480

Query: 481 AALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRF 540
           AALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRF
Sbjct: 481 AALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRF 540

Query: 541 QAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDAD 600
           QAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDAD
Sbjct: 541 QAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDAD 600

Query: 601 VDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFP 660
           VDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFP
Sbjct: 601 VDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFP 660

Query: 661 QNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRK 720
           QNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRK
Sbjct: 661 QNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRK 720

Query: 721 ELIQKSANLINIGHLDI 735
           ELIQKSANLINIGHLDI
Sbjct: 721 ELIQKSANLINIGHLDI 737

BLAST of MC01g0230 vs. ExPASy TrEMBL
Match: A0A6J1EXI8 (DNA gyrase subunit B OS=Cucurbita moschata OX=3662 GN=LOC111439391 PE=3 SV=1)

HSP 1 Score: 1293 bits (3345), Expect = 0.0
Identity = 665/740 (89.86%), Postives = 697/740 (94.19%), Query Frame = 0

Query: 1   MALPLRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTG 60
           MAL    LLLK PPY  LRLMASS  L PSS+HLRSL+S+AS AP  +FL+ SGT FR  
Sbjct: 1   MALRNLLLLLKTPPYLHLRLMASSTSLFPSSIHLRSLTSAASRAPSFSFLEFSGTRFRAR 60

Query: 61  KI-SGIRLVQNVV-SAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYI 120
           KI + +RL QNVV SAKA MSSSAAT+T  GSESLKAYGSEQIQ    L+PVRKRPGMYI
Sbjct: 61  KIPTSLRLAQNVVVSAKASMSSSAATKTSPGSESLKAYGSEQIQVLEGLDPVRKRPGMYI 120

Query: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRK 180
           GSTGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHPAT+K
Sbjct: 121 GSTGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPATKK 180

Query: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYS 240
           SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEVC+WRDGKEFHQ YS
Sbjct: 181 SSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYS 240

Query: 241 RGKPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300
           RGKP TTLTCHVPS+ELK  QGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT
Sbjct: 241 RGKPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLT 300

Query: 301 ITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQW 360
           ITL KED+D+E NQYNEYCYAGGLVEYVAWLNSDKKPLHD VFGFRKETDGVAID+ALQW
Sbjct: 301 ITLTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHD-VFGFRKETDGVAIDIALQW 360

Query: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVRE 420
           CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGK+SKIIKDKDISLSGEHVRE
Sbjct: 361 CADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKRSKIIKDKDISLSGEHVRE 420

Query: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLN 480
           GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHP+VLDLILSKSLN
Sbjct: 421 GLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLN 480

Query: 481 ALKAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRD 540
           ALKAALAAKRARELVR+K+VLRSSSLPGKLADC+ST+PE+SEIFIVEGDSAGGSAKQGRD
Sbjct: 481 ALKAALAAKRARELVRQKSVLRSSSLPGKLADCSSTDPEDSEIFIVEGDSAGGSAKQGRD 540

Query: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILT 600
           RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF+KE LRYHK+IILT
Sbjct: 541 RRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILT 600

Query: 601 DADVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQS 660
           DADVDGAHIRTLLLTFFFRYQKAL+D+GCIYIGVPPLYKVERGKQ YYCYDD ELK LQS
Sbjct: 601 DADVDGAHIRTLLLTFFFRYQKALFDQGCIYIGVPPLYKVERGKQAYYCYDDKELKRLQS 660

Query: 661 TFPQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVD 720
           +FP NASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQL +EDAAEANVVFSSLMGARVD
Sbjct: 661 SFPSNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLAIEDAAEANVVFSSLMGARVD 720

Query: 721 FRKELIQKSANLINIGHLDI 735
           FRKELIQKSAN+I++GHLDI
Sbjct: 721 FRKELIQKSANMIDLGHLDI 739

BLAST of MC01g0230 vs. ExPASy TrEMBL
Match: A0A6J1HVA6 (DNA gyrase subunit B OS=Cucurbita maxima OX=3661 GN=LOC111467169 PE=3 SV=1)

HSP 1 Score: 1289 bits (3335), Expect = 0.0
Identity = 664/738 (89.97%), Postives = 693/738 (93.90%), Query Frame = 0

Query: 5   LRHLL--LKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKI 64
           LR+LL  LK PPY  LRLMASS  L PSS+HLRSL+SSAS APP +FL+ SGT FR  KI
Sbjct: 3   LRNLLHLLKTPPYLHLRLMASSASLFPSSIHLRSLTSSASRAPPFSFLEFSGTRFRARKI 62

Query: 65  -SGIRLVQNVV-SAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGS 124
            + +RL QNVV SA AFMSSSAAT+T  GSES KAYGSEQIQ    L+PVRKRPGMYIGS
Sbjct: 63  PTSLRLAQNVVVSATAFMSSSAATKTSPGSESSKAYGSEQIQVLEGLDPVRKRPGMYIGS 122

Query: 125 TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSS 184
           TGPRGLHHLVYEILDNAVDEAQAGFAS+IDVVLHADNSVSITDNGRGIPTDLHP T+KSS
Sbjct: 123 TGPRGLHHLVYEILDNAVDEAQAGFASKIDVVLHADNSVSITDNGRGIPTDLHPVTKKSS 182

Query: 185 LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRG 244
           LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEVC+WRDGKEFHQ YSRG
Sbjct: 183 LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVCIWRDGKEFHQKYSRG 242

Query: 245 KPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTIT 304
           KP TTLTCHVPS+ELK  QGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTIT
Sbjct: 243 KPATTLTCHVPSNELKDHQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTIT 302

Query: 305 LKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCA 364
           L KED+D+E NQYNEYCYAGGLVEYVAWLNSDKKPLHD VFGFRKETDGVAID+ALQWCA
Sbjct: 303 LTKEDDDVEKNQYNEYCYAGGLVEYVAWLNSDKKPLHD-VFGFRKETDGVAIDIALQWCA 362

Query: 365 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 424
           DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL
Sbjct: 363 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 422

Query: 425 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNAL 484
           TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHP+VLDLILSKSLNAL
Sbjct: 423 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPDVLDLILSKSLNAL 482

Query: 485 KAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRR 544
           KAALAAKRARELVR+K+VLRSSSLPGKLADC+STNPE+SEIFIVEGDSAGGSAKQGRDRR
Sbjct: 483 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSTNPEDSEIFIVEGDSAGGSAKQGRDRR 542

Query: 545 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDA 604
           FQAILPLRGKILNIERKDEAAMYKNEEIQNLIL LGLGVKGEDF+KE LRYHK+IILTDA
Sbjct: 543 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILALGLGVKGEDFKKEALRYHKIIILTDA 602

Query: 605 DVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTF 664
           DVDGAHIRTLLLTFFFRYQKAL+DEGCIYIGVPPLYKVERGK+ YYCYDD ELK LQ +F
Sbjct: 603 DVDGAHIRTLLLTFFFRYQKALFDEGCIYIGVPPLYKVERGKKAYYCYDDKELKRLQRSF 662

Query: 665 PQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFR 724
             NASYNIQRFKGLGEMMP QLWETTM+PEQRMLKQL VEDAAEANVVFSSLMGARVDFR
Sbjct: 663 SSNASYNIQRFKGLGEMMPVQLWETTMNPEQRMLKQLAVEDAAEANVVFSSLMGARVDFR 722

Query: 725 KELIQKSANLINIGHLDI 735
           KELIQKSAN+IN+GHLDI
Sbjct: 723 KELIQKSANMINLGHLDI 739

BLAST of MC01g0230 vs. ExPASy TrEMBL
Match: A0A5D3D861 (DNA gyrase subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1676G00010 PE=3 SV=1)

HSP 1 Score: 1264 bits (3272), Expect = 0.0
Identity = 656/738 (88.89%), Postives = 685/738 (92.82%), Query Frame = 0

Query: 5   LRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQL--SGTSFRTGKI 64
           LR LLL+APPY PLRLMASS  L PSS+HLR LSSSAS+AP  +FLQL  SG  FRT KI
Sbjct: 3   LRQLLLRAPPYLPLRLMASSTSLFPSSIHLRFLSSSASIAPRFSFLQLRTSGGRFRTMKI 62

Query: 65  S-GIRLVQNV-VSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGS 124
           S  +RLV NV VSAKAFMSS+AATET TGSES KAYGSEQIQ    L+PVRKRPGMYIGS
Sbjct: 63  STSLRLVPNVEVSAKAFMSSTAATETSTGSESSKAYGSEQIQVLEGLDPVRKRPGMYIGS 122

Query: 125 TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSS 184
           TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHP T+KSS
Sbjct: 123 TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPVTKKSS 182

Query: 185 LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRG 244
           LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEV +WRDGKEFHQ YSRG
Sbjct: 183 LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGKEFHQKYSRG 242

Query: 245 KPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTIT 304
           KP + LT HVPSSELK+ QGTRIRFWPDKEVFTT IQFDYNTISGRIRELAFLNPKLTIT
Sbjct: 243 KPASILTSHVPSSELKNHQGTRIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPKLTIT 302

Query: 305 LKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCA 364
           L KEDND E NQYNEYCYAGGLVEYVAWLNSDK P+HD VFGFRKETDGVAIDVALQWCA
Sbjct: 303 LTKEDNDREKNQYNEYCYAGGLVEYVAWLNSDKNPIHD-VFGFRKETDGVAIDVALQWCA 362

Query: 365 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 424
           DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSK  KDKDISLSGEHVREGL
Sbjct: 363 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKTFKDKDISLSGEHVREGL 422

Query: 425 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNAL 484
           TCVVSVRV+NPEFEGQTKTRLGNPEVRKVVEQSIQE+LTEYLELHP+VLD ILSKSLNAL
Sbjct: 423 TCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEHLTEYLELHPDVLDSILSKSLNAL 482

Query: 485 KAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRR 544
           KAALAAKRARELVR+K+VLRSSSLPGKLADC+S  PEESEIFIVEGDSAGGSAKQGRDRR
Sbjct: 483 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGRDRR 542

Query: 545 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDA 604
           FQAILPLRGKILNIERKDEAAMYKNEEI NLI  LGLG KGEDF+KE LRYHK+IILTDA
Sbjct: 543 FQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKEALRYHKIIILTDA 602

Query: 605 DVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTF 664
           DVDGAHIRTLLLTFFFRYQKAL+DEGCIY+GVPPL+KVERGKQVYYCYDD ELK +QS+F
Sbjct: 603 DVDGAHIRTLLLTFFFRYQKALFDEGCIYVGVPPLFKVERGKQVYYCYDDKELKKVQSSF 662

Query: 665 PQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFR 724
           P NASYNIQRFKGLGEMMPAQLWETTMDPE+RMLKQL VEDAAEANVVFSSLMGARVDFR
Sbjct: 663 PSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGARVDFR 722

Query: 725 KELIQKSANLINIGHLDI 735
           KELIQKSA+ +N+ HLDI
Sbjct: 723 KELIQKSASTMNLEHLDI 739

BLAST of MC01g0230 vs. ExPASy TrEMBL
Match: A0A1S3C142 (DNA gyrase subunit B OS=Cucumis melo OX=3656 GN=LOC103495821 PE=3 SV=1)

HSP 1 Score: 1263 bits (3268), Expect = 0.0
Identity = 655/738 (88.75%), Postives = 685/738 (92.82%), Query Frame = 0

Query: 5   LRHLLLKAPPYFPLRLMASSF-LLPSSLHLRSLSSSASLAPPATFLQL--SGTSFRTGKI 64
           LR LLL+APPY PLRLMASS  L PSS+HLR LSSSAS+AP  +FLQL  SG  FRT KI
Sbjct: 3   LRQLLLRAPPYLPLRLMASSTSLFPSSIHLRFLSSSASIAPRFSFLQLRTSGGRFRTMKI 62

Query: 65  S-GIRLVQNV-VSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGS 124
           S  +RLV NV VSAKAFMSS+AATET TGSES KAYGSEQIQ    L+PVRKRPGMYIGS
Sbjct: 63  STSLRLVPNVEVSAKAFMSSTAATETSTGSESSKAYGSEQIQVLEGLDPVRKRPGMYIGS 122

Query: 125 TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSS 184
           TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHP T+KSS
Sbjct: 123 TGPRGLHHLVYEILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPVTKKSS 182

Query: 185 LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRG 244
           LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE LEV +WRDGKEFHQ YSRG
Sbjct: 183 LETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSESLEVSIWRDGKEFHQKYSRG 242

Query: 245 KPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTIT 304
           KP + LT HVPSSELK+ QGTRIRFWPDKEVFTT IQFDYNTISGRIRELAFLNPKLTIT
Sbjct: 243 KPASILTSHVPSSELKNHQGTRIRFWPDKEVFTTGIQFDYNTISGRIRELAFLNPKLTIT 302

Query: 305 LKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCA 364
           L KEDND E NQYNEYCYAGGLVEYVAWLNSDK P+HD VFGFRKETDGVAIDVALQWCA
Sbjct: 303 LTKEDNDREKNQYNEYCYAGGLVEYVAWLNSDKNPIHD-VFGFRKETDGVAIDVALQWCA 362

Query: 365 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGL 424
           DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSK  KDKDISLSGEHVREGL
Sbjct: 363 DAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKTFKDKDISLSGEHVREGL 422

Query: 425 TCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNAL 484
           TCVVSVRV+NPEFEGQTKTRLGNPEVRKVVEQSIQE+LTEYLELHP+VLD ILSKSLNAL
Sbjct: 423 TCVVSVRVRNPEFEGQTKTRLGNPEVRKVVEQSIQEHLTEYLELHPDVLDSILSKSLNAL 482

Query: 485 KAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRR 544
           KAALAAKRARELVR+K+VLRSSSLPGKLADC+S  PEESEIFIVEGDSAGGSAKQGRDRR
Sbjct: 483 KAALAAKRARELVRQKSVLRSSSLPGKLADCSSIKPEESEIFIVEGDSAGGSAKQGRDRR 542

Query: 545 FQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDA 604
           FQAILPLRGKILNIERKDEAAMYKNEEI NLI  LGLG KGEDF+KE LRYHK+IILTDA
Sbjct: 543 FQAILPLRGKILNIERKDEAAMYKNEEINNLIRALGLGFKGEDFKKEALRYHKIIILTDA 602

Query: 605 DVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTF 664
           DVDGAHIRTLLLTFFFRYQKAL++EGCIY+GVPPL+KVERGKQVYYCYDD ELK +QS+F
Sbjct: 603 DVDGAHIRTLLLTFFFRYQKALFEEGCIYVGVPPLFKVERGKQVYYCYDDKELKKVQSSF 662

Query: 665 PQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFR 724
           P NASYNIQRFKGLGEMMPAQLWETTMDPE+RMLKQL VEDAAEANVVFSSLMGARVDFR
Sbjct: 663 PSNASYNIQRFKGLGEMMPAQLWETTMDPERRMLKQLAVEDAAEANVVFSSLMGARVDFR 722

Query: 725 KELIQKSANLINIGHLDI 735
           KELIQKSA+ +N+ HLDI
Sbjct: 723 KELIQKSASTMNLEHLDI 739

BLAST of MC01g0230 vs. TAIR 10
Match: AT5G04130.1 (DNA GYRASE B2 )

HSP 1 Score: 1077.4 bits (2785), Expect = 0.0e+00
Identity = 543/719 (75.52%), Postives = 614/719 (85.40%), Query Frame = 0

Query: 19  RLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKISGIRLVQNVVSAKAFMS 78
           RL +SS       H  +LSS    +P  +F       F+   +   RL+Q    +  F+S
Sbjct: 23  RLFSSSLSPALHRHSSTLSSPPFSSPSPSF----RLKFQLTSVLSQRLIQRNAISSRFLS 82

Query: 79  SSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLHHLVYEILDNAVD 138
           + A+ ET T     K Y SEQIQ    L+PVRKRPGMYIGSTG RGLHHLVYEILDNA+D
Sbjct: 83  TEASQETTTS----KGYSSEQIQVLEGLDPVRKRPGMYIGSTGSRGLHHLVYEILDNAID 142

Query: 139 EAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLETVLTVLHAGGKFGGSSS 198
           EAQAG+AS++DVVLHAD SVS+ DNGRGIPTDLHPAT+KSSLETVLTVLHAGGKFGG+SS
Sbjct: 143 EAQAGYASKVDVVLHADGSVSVVDNGRGIPTDLHPATKKSSLETVLTVLHAGGKFGGTSS 202

Query: 199 GYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLTCHVPSSELKHRQ 258
           GYSVSGGLHGVGLSVVNALSE LEV VWRDG E  QNYSRGKP+TTLTC V   E K  +
Sbjct: 203 GYSVSGGLHGVGLSVVNALSEALEVSVWRDGMEHKQNYSRGKPITTLTCRVLPLESKGTK 262

Query: 259 GTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLKKEDNDLENNQYNEYCYA 318
           GT IRFWPDKEVFTT I+FD+NTI+GRIRELAFLNPK+TI+LKKED+D E  QY+EY +A
Sbjct: 263 GTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTISLKKEDDDPEKTQYSEYSFA 322

Query: 319 GGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDG 378
           GGL EYV+WLN+DK P+H DV GFR+E +G  +DVALQWC+DAYSDT+LGYANSIRTIDG
Sbjct: 323 GGLTEYVSWLNTDKNPIH-DVLGFRREINGATVDVALQWCSDAYSDTMLGYANSIRTIDG 382

Query: 379 GTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKT 438
           GTHI+G+KASLTRTLN L KKSK +K+KDISLSGEHVREGLTC+VSV+V NPEFEGQTKT
Sbjct: 383 GTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKT 442

Query: 439 RLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKAALAAKRARELVRKKNVL 498
           RLGNPEVRK+V+QS+QEYLTE+LELHP++L+ I+SKSLNA KAALAAKRARELVR K+VL
Sbjct: 443 RLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKSLNAYKAALAAKRARELVRSKSVL 502

Query: 499 RSSSLPGKLADCASTNPEESEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDE 558
           +SSSLPGKLADC+ST+PE SEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDE
Sbjct: 503 KSSSLPGKLADCSSTDPEVSEIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDE 562

Query: 559 AAMYKNEEIQNLILGLGLGVKGEDFRKEDLRYHKVIILTDADVDGAHIRTLLLTFFFRYQ 618
           AAMYKNEEIQNLILGLGLGVKGEDF+KE+LRYHK+IILTDADVDGAHIRTLLLTFFFRYQ
Sbjct: 563 AAMYKNEEIQNLILGLGLGVKGEDFKKENLRYHKIIILTDADVDGAHIRTLLLTFFFRYQ 622

Query: 619 KALYDEGCIYIGVPPLYKVERGKQVYYCYDDIELKNLQSTFPQNASYNIQRFKGLGEMMP 678
           +AL+D GCIY+GVPPL+KVERGK   YCYDD +LK + S FP NASYNIQRFKGLGEMMP
Sbjct: 623 RALFDAGCIYVGVPPLFKVERGKNAQYCYDDADLKKITSNFPANASYNIQRFKGLGEMMP 682

Query: 679 AQLWETTMDPEQRMLKQLTVEDAAEANVVFSSLMGARVDFRKELIQKSANLINIGHLDI 736
            QLWETTM+PE R+LKQL V+D AEAN+ FSSLMGARVD RKELI+ +A  IN+  LDI
Sbjct: 683 EQLWETTMNPETRILKQLVVDDIAEANMTFSSLMGARVDVRKELIKNAATRINLQRLDI 732

BLAST of MC01g0230 vs. TAIR 10
Match: AT3G10270.1 (DNA GYRASE B1 )

HSP 1 Score: 1062.0 bits (2745), Expect = 2.2e-310
Identity = 558/759 (73.52%), Postives = 624/759 (82.21%), Query Frame = 0

Query: 19  RLMAS-------SFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKISGIRLVQ-NV 78
           RLMAS       S  L  SLH  S + S+S              F+   +   RLVQ N 
Sbjct: 26  RLMASRPRPRLFSHSLYPSLHRHSSALSSS---------TPRIKFQLANVFSQRLVQRNA 85

Query: 79  VSAKAFMSSSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLHHLVY 138
           VS K+FMSS+   E+   S + K Y SE IQ    L+PVRKRPGMYIGSTG RGLHHLVY
Sbjct: 86  VSPKSFMSST--MESLQESSTSKDYSSEHIQVLEGLDPVRKRPGMYIGSTGSRGLHHLVY 145

Query: 139 EILDNAVDEAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLETVLTVLHAG 198
           EILDNA+DEAQAGFAS+IDVVLH+D+SVSI+DNGRGIPTDLHPAT KSSLETVLTVLHAG
Sbjct: 146 EILDNAIDEAQAGFASKIDVVLHSDDSVSISDNGRGIPTDLHPATGKSSLETVLTVLHAG 205

Query: 199 GKFGGSSSGYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLTCHVP 258
           GKFGG SSGYSVSGGLHGVGLSVVNALSE LEV V RDG EF Q YSRGKPVTTLTCHV 
Sbjct: 206 GKFGGKSSGYSVSGGLHGVGLSVVNALSEALEVIVRRDGMEFQQKYSRGKPVTTLTCHVL 265

Query: 259 SSELKHRQGTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLKKEDNDLENN 318
             E +  QGT IRFWPDKEVFTT IQFD+NTI+GRIRELAFLNPK+TI+LKKED+D E +
Sbjct: 266 PPESRGTQGTCIRFWPDKEVFTTAIQFDHNTIAGRIRELAFLNPKVTISLKKEDDDPERD 325

Query: 319 QYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADAYSDTILGYA 378
            Y+EY YAGGL EYV+WLN+DKKPLH DV GFRKE +G  +DV+LQWC+DAYSDT+LGYA
Sbjct: 326 VYSEYFYAGGLTEYVSWLNTDKKPLH-DVLGFRKEINGSTVDVSLQWCSDAYSDTMLGYA 385

Query: 379 NSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIK-------------------------- 438
           NSIRTIDGGTHI+G+KASLTRTLN+L KK K+IK                          
Sbjct: 386 NSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKVKGYIIYSSPWIETKFKYSSALKRHA 445

Query: 439 ------DKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLT 498
                 +KDISLSGEHVREGLTC+VSV+V NPEFEGQTKTRLGNPEVRK+V+QS+QEYLT
Sbjct: 446 LRTINNEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKTRLGNPEVRKIVDQSVQEYLT 505

Query: 499 EYLELHPNVLDLILSKSLNALKAALAAKRARELVRKKNVLRSSSLPGKLADCASTNPEES 558
           EYLELHP+VL+ I+SKSLNA KAALAAKRARELVR K+VL+SSSLPGKLADC+ST+P ES
Sbjct: 506 EYLELHPDVLESIISKSLNAYKAALAAKRARELVRSKSVLKSSSLPGKLADCSSTDPAES 565

Query: 559 EIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGV 618
           EIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGV
Sbjct: 566 EIFIVEGDSAGGSAKQGRDRRFQAILPLRGKILNIERKDEAAMYKNEEIQNLILGLGLGV 625

Query: 619 KGEDFRKEDLRYHKVIILTDADVDGAHIRTLLLTFFFRYQKALYDEGCIYIGVPPLYKVE 678
           KGEDF KE+LRYHK+IILTDADVDGAHIRTLLLTFFFRYQ+AL+D GCIY+GVPPL+KVE
Sbjct: 626 KGEDFNKENLRYHKIIILTDADVDGAHIRTLLLTFFFRYQRALFDAGCIYVGVPPLFKVE 685

Query: 679 RGKQVYYCYDDIELKNLQSTFPQNASYNIQRFKGLGEMMPAQLWETTMDPEQRMLKQLTV 736
           RGKQ +YCYDD  LK + ++FP NASYNIQRFKGLGEMMPAQLWETTM+P+ R+LKQL V
Sbjct: 686 RGKQAHYCYDDAALKKITASFPGNASYNIQRFKGLGEMMPAQLWETTMNPDTRILKQLVV 745

BLAST of MC01g0230 vs. TAIR 10
Match: AT5G04130.2 (DNA GYRASE B2 )

HSP 1 Score: 705.3 bits (1819), Expect = 5.2e-203
Identity = 360/503 (71.57%), Postives = 417/503 (82.90%), Query Frame = 0

Query: 19  RLMASSFLLPSSLHLRSLSSSASLAPPATFLQLSGTSFRTGKISGIRLVQNVVSAKAFMS 78
           RL +SS       H  +LSS    +P  +F       F+   +   RL+Q    +  F+S
Sbjct: 23  RLFSSSLSPALHRHSSTLSSPPFSSPSPSF----RLKFQLTSVLSQRLIQRNAISSRFLS 82

Query: 79  SSAATETPTGSESLKAYGSEQIQA--SLEPVRKRPGMYIGSTGPRGLHHLVYEILDNAVD 138
           + A+ ET T     K Y SEQIQ    L+PVRKRPGMYIGSTG RGLHHLVYEILDNA+D
Sbjct: 83  TEASQETTTS----KGYSSEQIQVLEGLDPVRKRPGMYIGSTGSRGLHHLVYEILDNAID 142

Query: 139 EAQAGFASQIDVVLHADNSVSITDNGRGIPTDLHPATRKSSLETVLTVLHAGGKFGGSSS 198
           EAQAG+AS++DVVLHAD SVS+ DNGRGIPTDLHPAT+KSSLETVLTVLHAGGKFGG+SS
Sbjct: 143 EAQAGYASKVDVVLHADGSVSVVDNGRGIPTDLHPATKKSSLETVLTVLHAGGKFGGTSS 202

Query: 199 GYSVSGGLHGVGLSVVNALSELLEVCVWRDGKEFHQNYSRGKPVTTLTCHVPSSELKHRQ 258
           GYSVSGGLHGVGLSVVNALSE LEV VWRDG E  QNYSRGKP+TTLTC V   E K  +
Sbjct: 203 GYSVSGGLHGVGLSVVNALSEALEVSVWRDGMEHKQNYSRGKPITTLTCRVLPLESKGTK 262

Query: 259 GTRIRFWPDKEVFTTVIQFDYNTISGRIRELAFLNPKLTITLKKEDNDLENNQYNEYCYA 318
           GT IRFWPDKEVFTT I+FD+NTI+GRIRELAFLNPK+TI+LKKED+D E  QY+EY +A
Sbjct: 263 GTSIRFWPDKEVFTTAIEFDHNTIAGRIRELAFLNPKVTISLKKEDDDPEKTQYSEYSFA 322

Query: 319 GGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDG 378
           GGL EYV+WLN+DK P+H DV GFR+E +G  +DVALQWC+DAYSDT+LGYANSIRTIDG
Sbjct: 323 GGLTEYVSWLNTDKNPIH-DVLGFRREINGATVDVALQWCSDAYSDTMLGYANSIRTIDG 382

Query: 379 GTHIDGMKASLTRTLNNLGKKSKIIKDKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKT 438
           GTHI+G+KASLTRTLN L KKSK +K+KDISLSGEHVREGLTC+VSV+V NPEFEGQTKT
Sbjct: 383 GTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGLTCIVSVKVPNPEFEGQTKT 442

Query: 439 RLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLILSKSLNALKAALAAKRARELVRKKNVL 498
           RLGNPEVRK+V+QS+QEYLTE+LELHP++L+ I+SKSLNA KAALAAKRARELVR K+VL
Sbjct: 443 RLGNPEVRKIVDQSVQEYLTEFLELHPDILESIISKSLNAYKAALAAKRARELVRSKSVL 502

Query: 499 RSSSLPGKLADCASTNPEESEIF 520
           +SSSLPGKLADC+ST+PE S ++
Sbjct: 503 KSSSLPGKLADCSSTDPEVSGMY 516

BLAST of MC01g0230 vs. TAIR 10
Match: AT5G04110.1 (DNA GYRASE B3 )

HSP 1 Score: 266.5 bits (680), Expect = 6.2e-71
Identity = 144/253 (56.92%), Postives = 184/253 (72.73%), Query Frame = 0

Query: 290 LNPKLTITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFGFRKETDGVAI 349
           L  ++T++L+ EDND       EY YA GL E+V WLN+DKKPLH DV GFRKE +G  I
Sbjct: 17  LRVQITVSLEMEDND----PNKEYLYAKGLSEFVTWLNADKKPLH-DVLGFRKEINGTTI 76

Query: 350 DVALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSKIIKDKDISLS 409
           ++ALQWC D YS+ ILGYAN IRT+DGGT+IDG+KAS+TRTLN+L +KSK+++DKDI  +
Sbjct: 77  NIALQWCVDGYSNKILGYANGIRTMDGGTYIDGVKASITRTLNSLVEKSKLVEDKDIIFT 136

Query: 410 GEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYLELHPNVLDLI 469
            EHV EGLTC+VSV V  PEFEGQT+ RLGNP VR++V+QS+QE L E  ELHP+V + I
Sbjct: 137 EEHVMEGLTCIVSVIVPKPEFEGQTQ-RLGNPNVREIVDQSVQECLMESFELHPDVFESI 196

Query: 470 LSKSLNALKAALAAKRARELVRKKN---VLRSSSLPGKLADCASTNPEESEIFIVEGDSA 529
           +SKS NA K  LA KRAR++   ++   V    S+P KL + +S   E SE FI  G S+
Sbjct: 197 MSKSYNAYKTDLAVKRARDVYSSESVAMVCAIESIPMKLTNSSS---ETSETFIGRGVSS 256

Query: 530 GGSAKQGRDRRFQ 540
           GG+AK   DR F+
Sbjct: 257 GGAAKHDSDRCFK 260

BLAST of MC01g0230 vs. TAIR 10
Match: AT3G23890.1 (topoisomerase II )

HSP 1 Score: 137.9 bits (346), Expect = 3.3e-32
Identity = 174/715 (24.34%), Postives = 298/715 (41.68%), Query Frame = 0

Query: 78  SSSAATETPTGSESLKAYGSEQIQ------ASLEPVRKRPGMYIGS-------------- 137
           +S+AA      ++S  A G + I+      + LE +  RP  YIGS              
Sbjct: 9   NSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLWVYEKD 68

Query: 138 -------TGPRGLHHLVYEILDNAVDEAQA-GFASQIDVVLHAD-NSVSITDNGRGIPTD 197
                  T   GL+ +  EIL NA D  Q       + VV+  + N +S+ ++G G+P +
Sbjct: 69  EMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGAGVPVE 128

Query: 198 LHPATRKSSLETVLTVLHAGGKFGGSSSGYSVSGGLHGVGLSVVNALSE--LLEVCVWRD 257
           +H        E +   L     +  +      +GG +G G  + N  S   ++E    + 
Sbjct: 129 IHQEEGIYVPEMIFGHLLTSSNYDDNVK--KTTGGRNGYGAKLTNIFSTEFIIETADGKR 188

Query: 258 GKEFHQNY-----SRGKPVTTLTCHVPSSELKHRQGTRIRFWPDKEVFT-TVIQFDYNTI 317
            K++ Q +      + +PV T  C+      K    T++ F PD + F  T ++ D   +
Sbjct: 189 LKKYKQVFENNMGKKSEPVIT-KCN------KSENWTKVTFKPDLKKFNMTELEDDVVAL 248

Query: 318 -SGRIRELAFLNPKLTITLKKEDNDLENNQYNEYCYAGGLVEYVAWLNSDKKPLHDDVFG 377
            S R+ ++A    K ++ ++     +    + +Y           +L++  K   +D   
Sbjct: 249 MSKRVFDIAGCLGK-SVKVELNGKQIPVKSFTDY--------VDLYLSAANKSRTEDPLP 308

Query: 378 FRKETDGVAIDVALQWCADAYSDTILGYANSIRTIDGGTHIDGMKASLTRTLNNLGKKSK 437
              E      +V +      +    + + NSI TI GGTH+D + + +T  +     K  
Sbjct: 309 RLTEKVNDRWEVCVSLSEGQFQQ--VSFVNSIATIKGGTHVDYVTSQITNHIVAAVNK-- 368

Query: 438 IIKDKDISLSGEHVREGLTCVVSVRVQNPEFEGQTKTRLGNPEVRKVVEQSIQEYLTEYL 497
             K+K+ ++   +V+  L   V+  + NP F+ QTK  L    +R+    S  E   ++L
Sbjct: 369 --KNKNANVKAHNVKNHLWVFVNALIDNPAFDSQTKETL---TLRQSSFGSKCELSEDFL 428

Query: 498 E------LHPNVLDLILSKSLNALKAALAAKRARELVRKKNVLRSSSLPGKLADCASTNP 557
           +      +  N+L     K    LK +  AK  R LV K +           A+    N 
Sbjct: 429 KKVGKSGVVENLLSWADFKQNKDLKKSDGAKTGRVLVEKLD---------DAAEAGGKNS 488

Query: 558 EESEIFIVEGDSAGGSAKQGRD---RRFQAILPLRGKILNIERKDEAAMYKNEEIQNL-- 617
               + + EGDSA   A  GR      +  + PLRGK+LN+       +  N+EI+NL  
Sbjct: 489 RLCTLILTEGDSAKSLALAGRSVLGNNYCGVFPLRGKLLNVREASTTQITNNKEIENLKK 548

Query: 618 ILGLGLGVKGEDFRKEDLRYHKVIILTDADVDGAHIRTLLLTFFFRYQKALYDEGCIYIG 677
           ILGL   +K E+     LRY +++I+TD D DG+HI+ LL+ F   +  +L       + 
Sbjct: 549 ILGLKQNMKYENV--NSLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVE 608

Query: 678 -VPPLYKVER--GKQVYYCYDDIELKNLQSTFPQNAS-YNIQRFKGLGEMMPAQ------ 725
            + P+ K  R   K+V   Y   E +  + +   NA+ ++I+ +KGLG     +      
Sbjct: 609 FITPIVKATRKGTKKVLSFYSMPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFS 668