MC01g0212 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0212
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPentatricopeptide repeat-containing protein
LocationMC01: 8568937 .. 8570848 (-)
RNA-Seq ExpressionMC01g0212
SyntenyMC01g0212
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATTCTCTCATCTGGGTTCGACACTGTCTCCACCGCCTCCAAGCTCGTACGCCTCTATGCCCACTTTGACGATCTTCCCAGTGCCGTCTCTGTTCTCAATGCCTTCCCTGAACCTGAACCCATGTTGTGGAACTCGATCATCAAGTCTCACGTTGAATCGGGTTTGTTTGTTTCTGCCCTTTTGTTATATAAAAAGATGAGGGAATTGGGAGTTGAGCACGATGGCTTTACGTTTCCGATGGTGAACCGAATTATTATGTCGATTCAGCTTGATGTGGTCTATGCGGGAATGGTTCACTGTGTTGGAATTCGGATGGGGTTTGGTGCGGATTTATATTTCTGTAATACAATGATGGAAGTTTATGGGAAATGTGGGTGTTTGGTTTCTGCTCGTAATGTGTTCGATGAAATGCCTCACAGAGACTTGGTTTCTTGGACATCGATGATTTCGGTGTATGTTTGTAGGGGTGATGTTGTTTCTGGTTTGGATCTTTTTGAGGGAATGAGGAGGGAGTTGGAGCCGAATTCGGTGACGATAATGGTGATGGTGCAAGCGTGCTGTGCGACTGGAAATTTGAGTCTGGGAAGGCAGCTTCAGAGTCATGTGTTTAAGAATGGTTTGTTGTTTGATATAGGTCTGCAGAATTCATTGTTGCGAATGTATACTCGTCTAGGCGGGGAAGATGAAGTTGGAGTTTTTTTCTCTGAAGTTGATCGCAAGAATGTTGTTTCTTGGAATCTTTTTATATCCTTTTATTCCTCTCGAGGGGATTTTGTGAAAGTTGTGGATATCTTCAACAAAATCATGGGTGAAGTTCTACTCAGCGTTGAGACACTAACCATACTTGTATCAGCAACCGCTGCACCTGATTCCGAGCATCTGATCCTAGGCAAAAATCTACATTCTCTAGCAATTAAAAGTGGCCTTTATGATGGTATTCTTCAGACTTCCTTTTTGGATATGTACGCCAAGTTTGGGGAGTTGGAAAATTCAACTAGGTTGTTTAAAGAAATCCCTCGTAAAAGCATCATCACCTGGGGAGCTATGATGTCTAGTTATTCAGAATGGACATTTTGATGGGGCGGTCGAGATCTTCAACCAAATGCAAGCTGCTGGCTTGAAACCCAGTGTTGGAATTTTAAAACACTTAATTGATGCATACACCCATTTGGGTGTTCTGCAATTGGGGAAAGCAATACATTGTTACCTTATCCGACTCAACGGTTTGGAGATCTATAATACGCAGTTAGGAACATCTATCCTTAACATGTATGTAAGATGTGGAAGCTTGGTTTCTGCTATAAAATGTTTTGATTTAATCTTAATCAAAGATGTTGTGGCATGGACTTCCATGATTGAAGGATATGGTGCTCATGGACTAGGTTTCGACGCCCTCAATCTGTTCCTTCAAATGATGAGAGAAGAAGTGACCCCAAATAATGTCACTTTCTTAAGTCTGTTATCTGCTTGCAGCCACTCTGGCCTTGTAAGCGAGGGCTGTCAAATCTTTTATTCAATGAGGTCAAGATTCAACATCAACCCTGATTTAGAACACTATACTTGTTTTGTTGATCTTTTGAGTAGATCAACAAGGGTAAGGGAGGCCTTTGCAATTATATTAAGAATGACAAATTTTCGTGATGGCAGGATATGGGGCGCTCTTATGGGTGCGTGCCGAGTGTATGAAGACAATAAAATCGCTAACTATGCTGCACACAGGCTTCTTGAATTAGAACCTGATAATGTAGGCTATTATACTTTGTTGAGCAATGCACAGGCTACTGTTGGGCAGTGGCATGACGTTGAAAAATTACGAAGTGTTGTGTACGAGAAAGATCTTGTCAAGAAACCGGGTTGGAGCTTCATTGAGTTAAAAGGAATAGTTCATGGATTTGTTTCAGGAGATAGA

mRNA sequence

ATCATTCTCTCATCTGGGTTCGACACTGTCTCCACCGCCTCCAAGCTCGTACGCCTCTATGCCCACTTTGACGATCTTCCCAGTGCCGTCTCTGTTCTCAATGCCTTCCCTGAACCTGAACCCATGTTGTGGAACTCGATCATCAAGTCTCACGTTGAATCGGGTTTGTTTGTTTCTGCCCTTTTGTTATATAAAAAGATGAGGGAATTGGGAGTTGAGCACGATGGCTTTACGTTTCCGATGGTGAACCGAATTATTATGTCGATTCAGCTTGATGTGGTCTATGCGGGAATGGTTCACTGTGTTGGAATTCGGATGGGGTTTGGTGCGGATTTATATTTCTGTAATACAATGATGGAAGTTTATGGGAAATGTGGGTGTTTGGTTTCTGCTCGTAATGTGTTCGATGAAATGCCTCACAGAGACTTGGTTTCTTGGACATCGATGATTTCGGTGTATGTTTGTAGGGGTGATGTTGTTTCTGGTTTGGATCTTTTTGAGGGAATGAGGAGGGAGTTGGAGCCGAATTCGGTGACGATAATGGTGATGGTGCAAGCGTGCTGTGCGACTGGAAATTTGAGTCTGGGAAGGCAGCTTCAGAGTCATGTGTTTAAGAATGGTTTGTTGTTTGATATAGGTCTGCAGAATTCATTGTTGCGAATGTATACTCGTCTAGGCGGGGAAGATGAAGTTGGAGTTTTTTTCTCTGAAGTTGATCGCAAGAATGTTGTTTCTTGGAATCTTTTTATATCCTTTTATTCCTCTCGAGGGGATTTTGTGAAAGTTGTGGATATCTTCAACAAAATCATGGGTGAAGTTCTACTCAGCGTTGAGACACTAACCATACTTGTATCAGCAACCGCTGCACCTGATTCCGAGCATCTGATCCTAGGCAAAAATCTACATTCTCTAGCAATTAAAAGTGGCCTTTATGATGGTATTCTTCAGACTTCCTTTTTGGATATGTACGCCAAGTTTGGGGAGTTGGAAAATTCAACTAGGTTGTTTAAAGAAATCCCTCGTAAAAGCATCATCACCTGGGGAGCTATGATGTCTAGTTATTCAGAAGGACATTTTGATGGGGCGGTCGAGATCTTCAACCAAATGCAAGCTGCTGGCTTGAAACCCAGTGTTGGAATTTTAAAACACTTAATTGATGCATACACCCATTTGGGTGTTCTGCAATTGGGGAAAGCAATACATTGTTACCTTATCCGACTCAACGGTTTGGAGATCTATAATACGCAGTTAGGAACATCTATCCTTAACATGTATGTAAGATGTGGAAGCTTGGTTTCTGCTATAAAATGTTTTGATTTAATCTTAATCAAAGATGTTGTGGCATGGACTTCCATGATTGAAGGATATGGTGCTCATGGACTAGGTTTCGACGCCCTCAATCTGTTCCTTCAAATGATGAGAGAAGAAGTGACCCCAAATAATGTCACTTTCTTAAGTCTGTTATCTGCTTGCAGCCACTCTGGCCTTGTAAGCGAGGGCTGTCAAATCTTTTATTCAATGAGGTCAAGATTCAACATCAACCCTGATTTAGAACACTATACTTGTTTTGTTGATCTTTTGAGTAGATCAACAAGGGTAAGGGAGGCCTTTGCAATTATATTAAGAATGACAAATTTTCGTGATGGCAGGATATGGGGCGCTCTTATGGGTGCGTGCCGAGTGTATGAAGACAATAAAATCGCTAACTATGCTGCACACAGGCTTCTTGAATTAGAACCTGATAATGTAGGCTATTATACTTTGTTGAGCAATGCACAGGCTACTGTTGGGCAGTGGCATGACGTTGAAAAATTACGAAGTGTTGTGTACGAGAAAGATCTTGTCAAGAAACCGGGTTGGAGCTTCATTGAGTTAAAAGGAATAGTTCATGGATTTGTTTCAGGAGATAGA

Coding sequence (CDS)

ATCATTCTCTCATCTGGGTTCGACACTGTCTCCACCGCCTCCAAGCTCGTACGCCTCTATGCCCACTTTGACGATCTTCCCAGTGCCGTCTCTGTTCTCAATGCCTTCCCTGAACCTGAACCCATGTTGTGGAACTCGATCATCAAGTCTCACGTTGAATCGGGTTTGTTTGTTTCTGCCCTTTTGTTATATAAAAAGATGAGGGAATTGGGAGTTGAGCACGATGGCTTTACGTTTCCGATGGTGAACCGAATTATTATGTCGATTCAGCTTGATGTGGTCTATGCGGGAATGGTTCACTGTGTTGGAATTCGGATGGGGTTTGGTGCGGATTTATATTTCTGTAATACAATGATGGAAGTTTATGGGAAATGTGGGTGTTTGGTTTCTGCTCGTAATGTGTTCGATGAAATGCCTCACAGAGACTTGGTTTCTTGGACATCGATGATTTCGGTGTATGTTTGTAGGGGTGATGTTGTTTCTGGTTTGGATCTTTTTGAGGGAATGAGGAGGGAGTTGGAGCCGAATTCGGTGACGATAATGGTGATGGTGCAAGCGTGCTGTGCGACTGGAAATTTGAGTCTGGGAAGGCAGCTTCAGAGTCATGTGTTTAAGAATGGTTTGTTGTTTGATATAGGTCTGCAGAATTCATTGTTGCGAATGTATACTCGTCTAGGCGGGGAAGATGAAGTTGGAGTTTTTTTCTCTGAAGTTGATCGCAAGAATGTTGTTTCTTGGAATCTTTTTATATCCTTTTATTCCTCTCGAGGGGATTTTGTGAAAGTTGTGGATATCTTCAACAAAATCATGGGTGAAGTTCTACTCAGCGTTGAGACACTAACCATACTTGTATCAGCAACCGCTGCACCTGATTCCGAGCATCTGATCCTAGGCAAAAATCTACATTCTCTAGCAATTAAAAGTGGCCTTTATGATGGTATTCTTCAGACTTCCTTTTTGGATATGTACGCCAAGTTTGGGGAGTTGGAAAATTCAACTAGGTTGTTTAAAGAAATCCCTCGTAAAAGCATCATCACCTGGGGAGCTATGATGTCTAGTTATTCAGAAGGACATTTTGATGGGGCGGTCGAGATCTTCAACCAAATGCAAGCTGCTGGCTTGAAACCCAGTGTTGGAATTTTAAAACACTTAATTGATGCATACACCCATTTGGGTGTTCTGCAATTGGGGAAAGCAATACATTGTTACCTTATCCGACTCAACGGTTTGGAGATCTATAATACGCAGTTAGGAACATCTATCCTTAACATGTATGTAAGATGTGGAAGCTTGGTTTCTGCTATAAAATGTTTTGATTTAATCTTAATCAAAGATGTTGTGGCATGGACTTCCATGATTGAAGGATATGGTGCTCATGGACTAGGTTTCGACGCCCTCAATCTGTTCCTTCAAATGATGAGAGAAGAAGTGACCCCAAATAATGTCACTTTCTTAAGTCTGTTATCTGCTTGCAGCCACTCTGGCCTTGTAAGCGAGGGCTGTCAAATCTTTTATTCAATGAGGTCAAGATTCAACATCAACCCTGATTTAGAACACTATACTTGTTTTGTTGATCTTTTGAGTAGATCAACAAGGGTAAGGGAGGCCTTTGCAATTATATTAAGAATGACAAATTTTCGTGATGGCAGGATATGGGGCGCTCTTATGGGTGCGTGCCGAGTGTATGAAGACAATAAAATCGCTAACTATGCTGCACACAGGCTTCTTGAATTAGAACCTGATAATGTAGGCTATTATACTTTGTTGAGCAATGCACAGGCTACTGTTGGGCAGTGGCATGACGTTGAAAAATTACGAAGTGTTGTGTACGAGAAAGATCTTGTCAAGAAACCGGGTTGGAGCTTCATTGAGTTAAAAGGAATAGTTCATGGATTTGTTTCAGGAGATAGA

Protein sequence

IILSSGFDTVSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSEGHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR
Homology
BLAST of MC01g0212 vs. ExPASy Swiss-Prot
Match: O49619 (Pentatricopeptide repeat-containing protein At4g35130, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H27 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 9.4e-105
Identity = 206/628 (32.80%), Postives = 349/628 (55.57%), Query Frame = 0

Query: 14  SKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRELGVE 73
           ++ +R +A    +  A+ + +   + +  LWN +IK     GL++ A+  Y +M   GV+
Sbjct: 68  TRALRGFADSRLMEDALQLFDEMNKADAFLWNVMIKGFTSCGLYIEAVQFYSRMVFAGVK 127

Query: 74  HDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEVYGKCGCLVSARN 133
            D FT+P V + +  I   +     +H + I++GF +D+Y CN+++ +Y K GC   A  
Sbjct: 128 ADTFTYPFVIKSVAGIS-SLEEGKKIHAMVIKLGFVSDVYVCNSLISLYMKLGCAWDAEK 187

Query: 134 VFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRR-ELEPNSVTIMVMVQACCATGN 193
           VF+EMP RD+VSW SMIS Y+  GD  S L LF+ M +   +P+  + M  + AC    +
Sbjct: 188 VFEEMPERDIVSWNSMISGYLALGDGFSSLMLFKEMLKCGFKPDRFSTMSALGACSHVYS 247

Query: 194 LSLGRQLQSHVFKNGL-LFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWNLFIS 253
             +G+++  H  ++ +   D+ +  S+L MY++ G        F+ + ++N+V+WN+ I 
Sbjct: 248 PKMGKEIHCHAVRSRIETGDVMVMTSILDMYSKYGEVSYAERIFNGMIQRNIVAWNVMIG 307

Query: 254 FYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIKSG-L 313
            Y+  G        F K+  +  L  + +T   S    P S  ++ G+ +H  A++ G L
Sbjct: 308 CYARNGRVTDAFLCFQKMSEQNGLQPDVIT---SINLLPASA-ILEGRTIHGYAMRRGFL 367

Query: 314 YDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSY-SEGHFDGAVEIFNQM 373
              +L+T+ +DMY + G+L+++  +F  +  K++I+W +++++Y   G    A+E+F ++
Sbjct: 368 PHMVLETALIDMYGECGQLKSAEVIFDRMAEKNVISWNSIIAAYVQNGKNYSALELFQEL 427

Query: 374 QAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRCG 433
             + L P    +  ++ AY     L  G+ IH Y+++       NT +  S+++MY  CG
Sbjct: 428 WDSSLVPDSTTIASILPAYAESLSLSEGREIHAYIVKSRYWS--NTIILNSLVHMYAMCG 487

Query: 434 SLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFLSLLS 493
            L  A KCF+ IL+KDVV+W S+I  Y  HG G  ++ LF +M+   V PN  TF SLL+
Sbjct: 488 DLEDARKCFNHILLKDVVSWNSIIMAYAVHGFGRISVWLFSEMIASRVNPNKSTFASLLA 547

Query: 494 ACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRDG 553
           ACS SG+V EG + F SM+  + I+P +EHY C +DL+ R+     A   +  M      
Sbjct: 548 ACSISGMVDEGWEYFESMKREYGIDPGIEHYGCMLDLIGRTGNFSAAKRFLEEMPFVPTA 607

Query: 554 RIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSVV 613
           RIWG+L+ A R ++D  IA +AA ++ ++E DN G Y LL N  A  G+W DV +++ ++
Sbjct: 608 RIWGSLLNASRNHKDITIAEFAAEQIFKMEHDNTGCYVLLLNMYAEAGRWEDVNRIKLLM 667

Query: 614 YEKDLVKKPGWSFIELKGIVHGFVSGDR 638
             K + +    S +E KG  H F +GDR
Sbjct: 668 ESKGISRTSSRSTVEAKGKSHVFTNGDR 688

BLAST of MC01g0212 vs. ExPASy Swiss-Prot
Match: Q9SN39 (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DOT4 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 5.9e-99
Identity = 218/628 (34.71%), Postives = 343/628 (54.62%), Query Frame = 0

Query: 14  SKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRELGVE 73
           SKL  +Y +  DL  A  V +     + + WN ++    +SG F  ++ L+KKM   GVE
Sbjct: 133 SKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVE 192

Query: 74  HDGFTFPMVNRIIMSIQLDVVYAG-MVHCVGIRMGFGADLYFCNTMMEVYGKCGCLVSAR 133
            D +TF  V++   S++   V+ G  +H   ++ GFG      N+++  Y K   + SAR
Sbjct: 193 MDSYTFSCVSKSFSSLR--SVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSAR 252

Query: 134 NVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMR-RELEPNSVTIMVMVQACCATG 193
            VFDEM  RD++SW S+I+ YV  G    GL +F  M    +E +  TI+ +   C  + 
Sbjct: 253 KVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSR 312

Query: 194 NLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWNLFIS 253
            +SLGR + S   K     +    N+LL MY++ G  D     F E+  ++VVS+   I+
Sbjct: 313 LISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIA 372

Query: 254 FYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIKSGL- 313
            Y+  G   + V +F + M E  +S +  T+            L  GK +H    ++ L 
Sbjct: 373 GYAREGLAGEAVKLFEE-MEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLG 432

Query: 314 YDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSEG-HFDGAVEIFN-Q 373
           +D  +  + +DMYAK G ++ +  +F E+  K II+W  ++  YS+  + + A+ +FN  
Sbjct: 433 FDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLL 492

Query: 374 MQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRC 433
           ++     P    +  ++ A   L     G+ IH Y++R NG    +  +  S+++MY +C
Sbjct: 493 LEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMR-NGY-FSDRHVANSLVDMYAKC 552

Query: 434 GSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFLSLL 493
           G+L+ A   FD I  KD+V+WT MI GYG HG G +A+ LF QM +  +  + ++F+SLL
Sbjct: 553 GALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLL 612

Query: 494 SACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRD 553
            ACSHSGLV EG + F  MR    I P +EHY C VD+L+R+  + +A+  I  M    D
Sbjct: 613 YACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPD 672

Query: 554 GRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSV 613
             IWGAL+  CR++ D K+A   A ++ ELEP+N GYY L++N  A   +W  V++LR  
Sbjct: 673 ATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKR 732

Query: 614 VYEKDLVKKPGWSFIELKGIVHGFVSGD 637
           + ++ L K PG S+IE+KG V+ FV+GD
Sbjct: 733 IGQRGLRKNPGCSWIEIKGRVNIFVAGD 755

BLAST of MC01g0212 vs. ExPASy Swiss-Prot
Match: Q9M9E2 (Pentatricopeptide repeat-containing protein At1g15510, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PCMP-H73 PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 3.8e-98
Identity = 210/632 (33.23%), Postives = 337/632 (53.32%), Query Frame = 0

Query: 10  VSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRE 69
           V   +  + ++  F +L  A  V     E     WN ++  + + G F  A+ LY +M  
Sbjct: 129 VELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLW 188

Query: 70  L-GVEHDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEVYGKCGCL 129
           + GV+ D +TFP V R    I  D+     VH   +R G+  D+   N ++ +Y KCG +
Sbjct: 189 VGGVKPDVYTFPCVLRTCGGIP-DLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDV 248

Query: 130 VSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMR-RELEPNSVTIMVMVQAC 189
            SAR +FD MP RD++SW +MIS Y   G    GL+LF  MR   ++P+ +T+  ++ AC
Sbjct: 249 KSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISAC 308

Query: 190 CATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWN 249
              G+  LGR + ++V   G   DI + NSL +MY   G   E    FS ++RK++VSW 
Sbjct: 309 ELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWT 368

Query: 250 LFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIK 309
             IS Y       K +D + ++M +  +  + +T+    +A      L  G  LH LAIK
Sbjct: 369 TMISGYEYNFLPDKAIDTY-RMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIK 428

Query: 310 SGLYD-GILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSEGHFDGAVEIF 369
           + L    I+  + ++MY+K   ++ +  +F  IPRK++I+W ++++     +      IF
Sbjct: 429 ARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIF 488

Query: 370 NQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLN-GLEIYNTQLGTSILNMY 429
            +     L+P+   L   + A   +G L  GK IH +++R   GL+ +   L  ++L+MY
Sbjct: 489 LRQMKMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDF---LPNALLDMY 548

Query: 430 VRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFL 489
           VRCG + +A   F+    KDV +W  ++ GY   G G   + LF +M++  V P+ +TF+
Sbjct: 549 VRCGRMNTAWSQFN-SQKKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFI 608

Query: 490 SLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTN 549
           SLL  CS S +V +G   F  M   + + P+L+HY C VDLL R+  ++EA   I +M  
Sbjct: 609 SLLCGCSKSQMVRQGLMYFSKMED-YGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPV 668

Query: 550 FRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKL 609
             D  +WGAL+ ACR++    +   +A  + EL+  +VGYY LL N  A  G+W +V K+
Sbjct: 669 TPDPAVWGALLNACRIHHKIDLGELSAQHIFELDKKSVGYYILLCNLYADCGKWREVAKV 728

Query: 610 RSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 638
           R ++ E  L    G S++E+KG VH F+S D+
Sbjct: 729 RRMMKENGLTVDAGCSWVEVKGKVHAFLSDDK 753

BLAST of MC01g0212 vs. ExPASy Swiss-Prot
Match: Q9SS97 (Putative pentatricopeptide repeat-containing protein At3g01580 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E87 PE=3 SV=2)

HSP 1 Score: 356.7 bits (914), Expect = 5.5e-97
Identity = 210/602 (34.88%), Postives = 330/602 (54.82%), Query Frame = 0

Query: 44  WNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVH-CV 103
           WN+++KS      +   L  +  M     + D FT P+  +    ++ +V Y  M+H  V
Sbjct: 28  WNTLLKSLSREKQWEEVLYHFSHMFRDEEKPDNFTLPVALKACGELR-EVNYGEMIHGFV 87

Query: 104 GIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSG 163
              +  G+DLY  ++++ +Y KCG ++ A  +FDE+   D+V+W+SM+S +   G     
Sbjct: 88  KKDVTLGSDLYVGSSLIYMYIKCGRMIEALRMFDELEKPDIVTWSSMVSGFEKNGSPYQA 147

Query: 164 LDLFEG--MRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLR 223
           ++ F    M  ++ P+ VT++ +V AC    N  LGR +   V + G   D+ L NSLL 
Sbjct: 148 VEFFRRMVMASDVTPDRVTLITLVSACTKLSNSRLGRCVHGFVIRRGFSNDLSLVNSLLN 207

Query: 224 MYTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIM--GEVLLSVE 283
            Y +     E    F  +  K+V+SW+  I+ Y   G   + + +FN +M  G       
Sbjct: 208 CYAKSRAFKEAVNLFKMIAEKDVISWSTVIACYVQNGAAAEALLVFNDMMDDGTEPNVAT 267

Query: 284 TLTILVSATAAPDSEHLILGKNLHSLAIKSGLYDGI-LQTSFLDMYAKFGELENSTRLFK 343
            L +L +  AA D E    G+  H LAI+ GL   + + T+ +DMY K    E +  +F 
Sbjct: 268 VLCVLQACAAAHDLEQ---GRKTHELAIRKGLETEVKVSTALVDMYMKCFSPEEAYAVFS 327

Query: 344 EIPRKSIITWGAMMSSYS-EGHFDGAVEIFNQMQAA-GLKPSVGILKHLIDAYTHLGVLQ 403
            IPRK +++W A++S ++  G    ++E F+ M      +P   ++  ++ + + LG L+
Sbjct: 328 RIPRKDVVSWVALISGFTLNGMAHRSIEEFSIMLLENNTRPDAILMVKVLGSCSELGFLE 387

Query: 404 LGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEG 463
             K  H Y+I+  G +  N  +G S++ +Y RCGSL +A K F+ I +KD V WTS+I G
Sbjct: 388 QAKCFHSYVIKY-GFD-SNPFIGASLVELYSRCGSLGNASKVFNGIALKDTVVWTSLITG 447

Query: 464 YGAHGLGFDALNLFLQMMR-EEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNIN 523
           YG HG G  AL  F  M++  EV PN VTFLS+LSACSH+GL+ EG +IF  M + + + 
Sbjct: 448 YGIHGKGTKALETFNHMVKSSEVKPNEVTFLSILSACSHAGLIHEGLRIFKLMVNDYRLA 507

Query: 524 PDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHR 583
           P+LEHY   VDLL R   +  A  I  RM      +I G L+GACR++++ ++A   A +
Sbjct: 508 PNLEHYAVLVDLLGRVGDLDTAIEITKRMPFSPTPQILGTLLGACRIHQNGEMAETVAKK 567

Query: 584 LLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVS 637
           L ELE ++ GYY L+SN     G+W +VEKLR+ V ++ + K    S IE++  VH FV+
Sbjct: 568 LFELESNHAGYYMLMSNVYGVKGEWENVEKLRNSVKQRGIKKGLAESLIEIRRKVHRFVA 623

BLAST of MC01g0212 vs. ExPASy Swiss-Prot
Match: Q9FLZ9 (Pentatricopeptide repeat-containing protein At5g39350 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E16 PE=2 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 9.4e-97
Identity = 213/638 (33.39%), Postives = 350/638 (54.86%), Query Frame = 0

Query: 2   ILSSGFDTVSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSAL 61
           +++ G  +    S L   YA    +  A  +    P+   + +N +I+ +V  GL+  A+
Sbjct: 41  VITGGRVSGHILSTLSVTYALCGHITYARKLFEEMPQSSLLSYNIVIRMYVREGLYHDAI 100

Query: 62  LLYKKMRELGVE--HDGFTFPMVNRI---IMSIQLDVVYAGMVHCVGIRMGFGADLYFCN 121
            ++ +M   GV+   DG+T+P V +    + S++L +V  G +    +R  FG D Y  N
Sbjct: 101 SVFIRMVSEGVKCVPDGYTYPFVAKAAGELKSMKLGLVVHGRI----LRSWFGRDKYVQN 160

Query: 122 TMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRRE-LEP 181
            ++ +Y   G +  AR+VFD M +RD++SW +MIS Y   G +   L +F+ M  E ++ 
Sbjct: 161 ALLAMYMNFGKVEMARDVFDVMKNRDVISWNTMISGYYRNGYMNDALMMFDWMVNESVDL 220

Query: 182 NSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFF 241
           +  TI+ M+  C    +L +GR +   V +  L   I ++N+L+ MY + G  DE    F
Sbjct: 221 DHATIVSMLPVCGHLKDLEMGRNVHKLVEEKRLGDKIEVKNALVNMYLKCGRMDEARFVF 280

Query: 242 SEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGE-VLLSVETLTILVSATAAPDSEH 301
             ++R++V++W   I+ Y+  GD    +++   +  E V  +  T+  LVS     D+  
Sbjct: 281 DRMERRDVITWTCMINGYTEDGDVENALELCRLMQFEGVRPNAVTIASLVSVCG--DALK 340

Query: 302 LILGKNLHSLAIKSGLY-DGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSS 361
           +  GK LH  A++  +Y D I++TS + MYAK   ++   R+F    +     W A+++ 
Sbjct: 341 VNDGKCLHGWAVRQQVYSDIIIETSLISMYAKCKRVDLCFRVFSGASKYHTGPWSAIIAG 400

Query: 362 YSEGHF-DGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEI 421
             +      A+ +F +M+   ++P++  L  L+ AY  L  L+    IHCYL +   +  
Sbjct: 401 CVQNELVSDALGLFKRMRREDVEPNIATLNSLLPAYAALADLRQAMNIHCYLTKTGFMS- 460

Query: 422 YNTQLGTSILNMYVRCGSLVSAIKCFDLI----LIKDVVAWTSMIEGYGAHGLGFDALNL 481
            +    T ++++Y +CG+L SA K F+ I      KDVV W ++I GYG HG G +AL +
Sbjct: 461 -SLDAATGLVHVYSKCGTLESAHKIFNGIQEKHKSKDVVLWGALISGYGMHGDGHNALQV 520

Query: 482 FLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLS 541
           F++M+R  VTPN +TF S L+ACSHSGLV EG  +F  M   +       HYTC VDLL 
Sbjct: 521 FMEMVRSGVTPNEITFTSALNACSHSGLVEEGLTLFRFMLEHYKTLARSNHYTCIVDLLG 580

Query: 542 RSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTL 601
           R+ R+ EA+ +I  +       +WGAL+ AC  +E+ ++   AA++L ELEP+N G Y L
Sbjct: 581 RAGRLDEAYNLITTIPFEPTSTVWGALLAACVTHENVQLGEMAANKLFELEPENTGNYVL 640

Query: 602 LSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELK 627
           L+N  A +G+W D+EK+RS++    L KKPG S IE++
Sbjct: 641 LANIYAALGRWKDMEKVRSMMENVGLRKKPGHSTIEIR 670

BLAST of MC01g0212 vs. NCBI nr
Match: XP_022153922.1 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1188 bits (3073), Expect = 0.0
Identity = 592/597 (99.16%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC
Sbjct: 1   MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS
Sbjct: 61  VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
           GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM
Sbjct: 121 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 281
           YTRLGGEDEVGVFFSEVDRKNVVSWN+FISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT
Sbjct: 181 YTRLGGEDEVGVFFSEVDRKNVVSWNVFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 240

Query: 282 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 341
           ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR
Sbjct: 241 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 300

Query: 342 KSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 401
           KSIITWGAMMSS+ + GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI
Sbjct: 301 KSIITWGAMMSSFIQNGHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 360

Query: 402 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 461
           HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG
Sbjct: 361 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 420

Query: 462 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 521
           LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY
Sbjct: 421 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 480

Query: 522 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 581
           TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP
Sbjct: 481 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 540

Query: 582 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR
Sbjct: 541 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 597

BLAST of MC01g0212 vs. NCBI nr
Match: KAG7033612.1 (Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1026 bits (2652), Expect = 0.0
Identity = 510/638 (79.94%), Postives = 561/638 (87.93%), Query Frame = 0

Query: 2   ILSSGFDTVSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSAL 61
           ILSSGFDTV  ASKL+RLY  F+DLPSAVSVLNAFP  EPMLWNSIIKS V+SGLF+SA+
Sbjct: 13  ILSSGFDTVFIASKLIRLYVKFNDLPSAVSVLNAFPHTEPMLWNSIIKSQVDSGLFLSAI 72

Query: 62  LLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEV 121
           +LYK MRE+GVEHDGFTFP++N ++MSI +DVVYAGMVHCVGIRMGFG+DLYFCNTMMEV
Sbjct: 73  MLYKNMREVGVEHDGFTFPILNHVVMSIWVDVVYAGMVHCVGIRMGFGSDLYFCNTMMEV 132

Query: 122 YGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRRELEPNSVTIM 181
           Y KC CL  AR VFDEMP+RDLVSWTSMIS YV  GD+V  L+LFEGMRR LEPNSVT+M
Sbjct: 133 YAKCECLGHARKVFDEMPNRDLVSWTSMISAYVNVGDIVCALNLFEGMRRVLEPNSVTMM 192

Query: 182 VMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRK 241
            M+QACC T +L LGR +Q  V KNGLLFD+GLQN  LRMY+RLGGEDE   FFSE+D K
Sbjct: 193 AMLQACCVTEDLVLGRLIQCLVVKNGLLFDVGLQNWFLRMYSRLGGEDEFVRFFSEIDCK 252

Query: 242 NVVSWNLFISFYSSRGDFVKVVDIFNKIM-GEVLLSVETLTILVSATAAPDSEHLILGKN 301
           NVVSW++ ISFYSS GD VK VDIF +IM GEV L +ETLTIL+SAT   DS  LILG+N
Sbjct: 253 NVVSWDILISFYSSVGDIVKAVDIFKQIMAGEVPLIIETLTILISATKTSDSMCLILGEN 312

Query: 302 LHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSE-GHF 361
           LHSLAIK+GLYD IL+TS LDMYAKFGEL+NSTRLF EIP +SIITWGAMMSS+ + GHF
Sbjct: 313 LHSLAIKTGLYDSILRTSLLDMYAKFGELDNSTRLFNEIPNRSIITWGAMMSSFIQNGHF 372

Query: 362 DGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGT 421
           D AVEIF+QMQAAGLKPS+GILKHLIDAY HLG LQLG+ IHCYLIR+ GLEI NT L T
Sbjct: 373 DEAVEIFSQMQAAGLKPSLGILKHLIDAYAHLGALQLGRGIHCYLIRICGLEICNTHLET 432

Query: 422 SILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTP 481
           S++NMYVRCGS+ SA KCFDLI++KDVVAWTSMIEGYGAHG G +ALNL+  MM EEV P
Sbjct: 433 SLMNMYVRCGSIASARKCFDLIIVKDVVAWTSMIEGYGAHGQGINALNLYHHMMSEEVAP 492

Query: 482 NNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAI 541
           N+VTFLSLLSACSHSGLVSEGC+IFYSMRSRFNI PDLEHYTCFVDLLSRSTRVREAFAI
Sbjct: 493 NSVTFLSLLSACSHSGLVSEGCEIFYSMRSRFNIKPDLEHYTCFVDLLSRSTRVREAFAI 552

Query: 542 ILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQW 601
           ILRMTN  DGRIWGALMGACRVY DNKIA YAAHRLLELEPDNVGYYTLLSN QA+VGQW
Sbjct: 553 ILRMTNLCDGRIWGALMGACRVYGDNKIAIYAAHRLLELEPDNVGYYTLLSNTQASVGQW 612

Query: 602 HDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           H+VEKLRSVVYEKDLVKKPGWSFIEL GI+HGFVSGDR
Sbjct: 613 HEVEKLRSVVYEKDLVKKPGWSFIELNGIIHGFVSGDR 650

BLAST of MC01g0212 vs. NCBI nr
Match: XP_023526509.1 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 508/638 (79.62%), Postives = 559/638 (87.62%), Query Frame = 0

Query: 2   ILSSGFDTVSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSAL 61
           ILSSGFDTV  ASKL+RLYA F+DLPSAVSVLNAFP  EPMLWNSIIKS  +SGLF+SA+
Sbjct: 25  ILSSGFDTVFIASKLIRLYAKFNDLPSAVSVLNAFPHTEPMLWNSIIKSQFDSGLFLSAI 84

Query: 62  LLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEV 121
           +LYK MRE+GVEHDGFTFP++N ++MSI +DVVYAGMVHCVGIRMGFG+DLYFCNTMMEV
Sbjct: 85  MLYKNMREVGVEHDGFTFPILNHVVMSIWVDVVYAGMVHCVGIRMGFGSDLYFCNTMMEV 144

Query: 122 YGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRRELEPNSVTIM 181
           Y KC CL  AR VFDEMP+RDLVSWTSMIS YV  GD+V  L+LFEGMRR LEPNSVT+M
Sbjct: 145 YAKCECLGHARKVFDEMPNRDLVSWTSMISAYVNSGDIVCALNLFEGMRRVLEPNSVTMM 204

Query: 182 VMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRK 241
            M+QACC T +L LGR +Q  V KNGLLFD+GLQN  LRMY+RLGGEDE   FFSE+D K
Sbjct: 205 AMLQACCVTEDLVLGRLIQCLVVKNGLLFDVGLQNWFLRMYSRLGGEDEFVCFFSEIDCK 264

Query: 242 NVVSWNLFISFYSSRGDFVKVVDIFNKIMG-EVLLSVETLTILVSATAAPDSEHLILGKN 301
           NVVSWN+ ISFYSS GD VK VDIF +IMG EV L +ETLTIL+SAT   +S  LILG+N
Sbjct: 265 NVVSWNILISFYSSVGDIVKAVDIFKQIMGGEVPLIIETLTILISATKTSESMCLILGEN 324

Query: 302 LHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSE-GHF 361
           LHSLAIK+GLYD IL+TS LDMYAKFGEL+NSTRLF EIP +SIITWGAMMSS+ + GHF
Sbjct: 325 LHSLAIKTGLYDSILRTSLLDMYAKFGELDNSTRLFNEIPNRSIITWGAMMSSFIQNGHF 384

Query: 362 DGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGT 421
           D AVEIF+QMQAAGLKPS+GILKHLIDAY HLG LQLG+ IHCYLIR+ GLEI NT L T
Sbjct: 385 DEAVEIFSQMQAAGLKPSLGILKHLIDAYAHLGALQLGRGIHCYLIRIYGLEICNTHLET 444

Query: 422 SILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTP 481
           S++NMYVRCGS+ SA KCFDLI++KDVVAWTSMIEGYGAHG G +ALNL+  MM EEV P
Sbjct: 445 SLMNMYVRCGSIASARKCFDLIIVKDVVAWTSMIEGYGAHGQGINALNLYHHMMSEEVAP 504

Query: 482 NNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAI 541
           N+VTFLSLLSACSHSGLVSEGC+IFYSMRSRFNI PDLEHYTCFVDLLSRSTRVREAFAI
Sbjct: 505 NSVTFLSLLSACSHSGLVSEGCEIFYSMRSRFNIKPDLEHYTCFVDLLSRSTRVREAFAI 564

Query: 542 ILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQW 601
           ILRMTN  DGRIWGALMGACRVY D KIA YAAHRLLELEPDNVGYYTLLSN QA+VGQW
Sbjct: 565 ILRMTNLCDGRIWGALMGACRVYGDTKIAIYAAHRLLELEPDNVGYYTLLSNTQASVGQW 624

Query: 602 HDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           H+VEKLRSVVYEKDLVKKPGWSFIEL G +HGFVSGDR
Sbjct: 625 HEVEKLRSVVYEKDLVKKPGWSFIELNGTIHGFVSGDR 662

BLAST of MC01g0212 vs. NCBI nr
Match: XP_038883286.1 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1005 bits (2599), Expect = 0.0
Identity = 499/597 (83.58%), Postives = 537/597 (89.95%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWNSIIKSH +SGLF+SALLLYK MRE+GVEHDGFTFP++N +IMSI +DV+YA MVHC
Sbjct: 1   MLWNSIIKSHFDSGLFLSALLLYKNMREVGVEHDGFTFPILNHVIMSIWVDVLYAEMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGF ADLYFCNTMMEVYGKCGCLV AR++FDEMP+RDLVSWTSMIS YV  GDVV 
Sbjct: 61  VGIRMGFIADLYFCNTMMEVYGKCGCLVYARHMFDEMPNRDLVSWTSMISAYVNGGDVVC 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
            LDLFE MRRELEPNSVT MVM+QACCAT N  LGRQLQ HV KNGLL DIGL+NS LRM
Sbjct: 121 ALDLFEAMRRELEPNSVTAMVMLQACCATQNFVLGRQLQCHVVKNGLLLDIGLRNSFLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 281
           Y+RLGGEDEVGVFFSE+D KNVVSWN+ +SFYSS G+ +KVVDIFNKIMGEV LS+ETLT
Sbjct: 181 YSRLGGEDEVGVFFSEIDCKNVVSWNILMSFYSSVGNILKVVDIFNKIMGEVTLSIETLT 240

Query: 282 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 341
           IL+SATA  DS  LILG+NLHSLAIKSGLYD ILQTS LDMYAKFGELENS +LFKEIP 
Sbjct: 241 ILISATATSDSGCLILGENLHSLAIKSGLYDSILQTSLLDMYAKFGELENSAKLFKEIPN 300

Query: 342 KSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 401
           +SIITWGAMMSS+ + GHFD AVEIF QMQAAGLKPSVG+LKHLIDAY +LG LQLGKAI
Sbjct: 301 RSIITWGAMMSSFIQNGHFDEAVEIFKQMQAAGLKPSVGVLKHLIDAYAYLGALQLGKAI 360

Query: 402 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 461
           HCYLIR+ GLEI NT L TS+LNMY RCGS+ SA KCFDLIL KDVV WTSMI+ YGAHG
Sbjct: 361 HCYLIRIYGLEICNTHLETSLLNMYGRCGSIASARKCFDLILTKDVVVWTSMIDVYGAHG 420

Query: 462 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 521
           LG DALNLF QMM EEV PN+VTFLSLLSACSHSGLVSEGC+IFYSMRS F+I PDLEHY
Sbjct: 421 LGIDALNLFHQMMSEEVAPNSVTFLSLLSACSHSGLVSEGCEIFYSMRSSFDIKPDLEHY 480

Query: 522 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 581
           TCFVDLLSRSTRVREAFAIILRMTN RDGRIWGALMGACRVY DNKIANYAAHRLLELEP
Sbjct: 481 TCFVDLLSRSTRVREAFAIILRMTNLRDGRIWGALMGACRVYGDNKIANYAAHRLLELEP 540

Query: 582 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           DNVGYYTLLSNAQA+VGQWH+VEKLRSVVYEKDLVKKPGWSFIEL G +HGFVSGDR
Sbjct: 541 DNVGYYTLLSNAQASVGQWHEVEKLRSVVYEKDLVKKPGWSFIELNGTIHGFVSGDR 597

BLAST of MC01g0212 vs. NCBI nr
Match: XP_008457591.1 (PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic [Cucumis melo] >XP_008457593.1 PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic [Cucumis melo] >XP_016902177.1 PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic [Cucumis melo] >XP_016902178.1 PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic [Cucumis melo] >XP_016902179.1 PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic [Cucumis melo] >XP_016902180.1 PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic [Cucumis melo])

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 491/597 (82.24%), Postives = 532/597 (89.11%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWN++IKSH +SGLF SALLLYK MRE+ VEHDGFT P+VN++I+SI +DVVY GMVHC
Sbjct: 1   MLWNNVIKSHFDSGLFHSALLLYKNMREVRVEHDGFTLPIVNQVILSIWVDVVYGGMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGF +DLYFCNTMMEVYGKCGCLVSAR+VFDEMP+RDLVSWTSMIS YV  GDV  
Sbjct: 61  VGIRMGFSSDLYFCNTMMEVYGKCGCLVSARDVFDEMPNRDLVSWTSMISAYVKGGDVFC 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
            LD+FEGMRRELEPNSVT++VM+QACCAT NL LGR LQ +V KNGLLFD GLQNS LRM
Sbjct: 121 ALDIFEGMRRELEPNSVTVIVMLQACCATQNLVLGRLLQCYVVKNGLLFDTGLQNSFLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 281
           Y+RLGGEDEV  FFSE+D KNVVSWN+ +SFYSS GD VKVVDI NKIMGEV LS+ETLT
Sbjct: 181 YSRLGGEDEVVAFFSEIDFKNVVSWNILMSFYSSMGDIVKVVDILNKIMGEVPLSIETLT 240

Query: 282 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 341
           IL+S  A  DS  LILG+NLHSLAIKSGLYD IL TS LDMYAKFGELENSTRLFKEIP 
Sbjct: 241 ILISGIATSDSGCLILGENLHSLAIKSGLYDDILCTSLLDMYAKFGELENSTRLFKEIPN 300

Query: 342 KSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 401
           +SIITWGAMMSS+ + GHFD AV+IF QMQ AGLKPSVGILKHLIDAY +LG LQLGKAI
Sbjct: 301 RSIITWGAMMSSFIQNGHFDDAVDIFKQMQVAGLKPSVGILKHLIDAYAYLGALQLGKAI 360

Query: 402 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 461
           HC+LIR+ GL + NT+L TS+LNMYVRCGS+ SA KCFDLILIKDVVAWTSMIEGYGAHG
Sbjct: 361 HCHLIRIYGLVVCNTRLETSVLNMYVRCGSIASARKCFDLILIKDVVAWTSMIEGYGAHG 420

Query: 462 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 521
           LG DALNLF QM  EEVTPNNVTFLSLLSACSHSGLVSEGC IFYSMRSRFNI PDLEHY
Sbjct: 421 LGIDALNLFHQMTSEEVTPNNVTFLSLLSACSHSGLVSEGCGIFYSMRSRFNIKPDLEHY 480

Query: 522 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 581
           TCFVDLLSRSTRVREAFAIILRMTN  DGRIWGALMGACRVY DNKIANYAAHRLLELEP
Sbjct: 481 TCFVDLLSRSTRVREAFAIILRMTNLCDGRIWGALMGACRVYGDNKIANYAAHRLLELEP 540

Query: 582 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           DNVGYYTLLSN+QA+VGQWH+ EKLRS+VYEK+L KKPGWSFIEL G +HGFVSGDR
Sbjct: 541 DNVGYYTLLSNSQASVGQWHEAEKLRSLVYEKNLAKKPGWSFIELNGTIHGFVSGDR 597

BLAST of MC01g0212 vs. ExPASy TrEMBL
Match: A0A6J1DKA1 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111021322 PE=4 SV=1)

HSP 1 Score: 1188 bits (3073), Expect = 0.0
Identity = 592/597 (99.16%), Postives = 595/597 (99.66%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC
Sbjct: 1   MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS
Sbjct: 61  VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
           GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM
Sbjct: 121 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 281
           YTRLGGEDEVGVFFSEVDRKNVVSWN+FISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT
Sbjct: 181 YTRLGGEDEVGVFFSEVDRKNVVSWNVFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 240

Query: 282 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 341
           ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR
Sbjct: 241 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 300

Query: 342 KSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 401
           KSIITWGAMMSS+ + GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI
Sbjct: 301 KSIITWGAMMSSFIQNGHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 360

Query: 402 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 461
           HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG
Sbjct: 361 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 420

Query: 462 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 521
           LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY
Sbjct: 421 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 480

Query: 522 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 581
           TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP
Sbjct: 481 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 540

Query: 582 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR
Sbjct: 541 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 597

BLAST of MC01g0212 vs. ExPASy TrEMBL
Match: A0A1S3C6I7 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497256 PE=4 SV=1)

HSP 1 Score: 992 bits (2564), Expect = 0.0
Identity = 491/597 (82.24%), Postives = 532/597 (89.11%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWN++IKSH +SGLF SALLLYK MRE+ VEHDGFT P+VN++I+SI +DVVY GMVHC
Sbjct: 1   MLWNNVIKSHFDSGLFHSALLLYKNMREVRVEHDGFTLPIVNQVILSIWVDVVYGGMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGF +DLYFCNTMMEVYGKCGCLVSAR+VFDEMP+RDLVSWTSMIS YV  GDV  
Sbjct: 61  VGIRMGFSSDLYFCNTMMEVYGKCGCLVSARDVFDEMPNRDLVSWTSMISAYVKGGDVFC 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
            LD+FEGMRRELEPNSVT++VM+QACCAT NL LGR LQ +V KNGLLFD GLQNS LRM
Sbjct: 121 ALDIFEGMRRELEPNSVTVIVMLQACCATQNLVLGRLLQCYVVKNGLLFDTGLQNSFLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLT 281
           Y+RLGGEDEV  FFSE+D KNVVSWN+ +SFYSS GD VKVVDI NKIMGEV LS+ETLT
Sbjct: 181 YSRLGGEDEVVAFFSEIDFKNVVSWNILMSFYSSMGDIVKVVDILNKIMGEVPLSIETLT 240

Query: 282 ILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPR 341
           IL+S  A  DS  LILG+NLHSLAIKSGLYD IL TS LDMYAKFGELENSTRLFKEIP 
Sbjct: 241 ILISGIATSDSGCLILGENLHSLAIKSGLYDDILCTSLLDMYAKFGELENSTRLFKEIPN 300

Query: 342 KSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAI 401
           +SIITWGAMMSS+ + GHFD AV+IF QMQ AGLKPSVGILKHLIDAY +LG LQLGKAI
Sbjct: 301 RSIITWGAMMSSFIQNGHFDDAVDIFKQMQVAGLKPSVGILKHLIDAYAYLGALQLGKAI 360

Query: 402 HCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHG 461
           HC+LIR+ GL + NT+L TS+LNMYVRCGS+ SA KCFDLILIKDVVAWTSMIEGYGAHG
Sbjct: 361 HCHLIRIYGLVVCNTRLETSVLNMYVRCGSIASARKCFDLILIKDVVAWTSMIEGYGAHG 420

Query: 462 LGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHY 521
           LG DALNLF QM  EEVTPNNVTFLSLLSACSHSGLVSEGC IFYSMRSRFNI PDLEHY
Sbjct: 421 LGIDALNLFHQMTSEEVTPNNVTFLSLLSACSHSGLVSEGCGIFYSMRSRFNIKPDLEHY 480

Query: 522 TCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEP 581
           TCFVDLLSRSTRVREAFAIILRMTN  DGRIWGALMGACRVY DNKIANYAAHRLLELEP
Sbjct: 481 TCFVDLLSRSTRVREAFAIILRMTNLCDGRIWGALMGACRVYGDNKIANYAAHRLLELEP 540

Query: 582 DNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           DNVGYYTLLSN+QA+VGQWH+ EKLRS+VYEK+L KKPGWSFIEL G +HGFVSGDR
Sbjct: 541 DNVGYYTLLSNSQASVGQWHEAEKLRSLVYEKNLAKKPGWSFIELNGTIHGFVSGDR 597

BLAST of MC01g0212 vs. ExPASy TrEMBL
Match: A0A6J1EYH5 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439622 PE=4 SV=1)

HSP 1 Score: 956 bits (2470), Expect = 0.0
Identity = 472/598 (78.93%), Postives = 523/598 (87.46%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWNSIIKS  +SGLF+SA++LYK MRE+GVEHDGFTFP++N ++MSI +DVVYAGMVHC
Sbjct: 1   MLWNSIIKSQFDSGLFLSAIMLYKNMREVGVEHDGFTFPILNHVVMSIWVDVVYAGMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGFG+DLYFCNTMMEVY KC CL  AR VFDEMP+RDLVSWTSMIS YV  GD+V 
Sbjct: 61  VGIRMGFGSDLYFCNTMMEVYAKCECLGHARKVFDEMPNRDLVSWTSMISAYVNVGDIVC 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
            L+LFEGMRR  EPNSVT+M M+QACC T +L LGR +Q  V KNGLLFD+GLQN  LRM
Sbjct: 121 ALNLFEGMRRVFEPNSVTMMAMLQACCVTEDLVLGRLIQCLVVKNGLLFDVGLQNWFLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIM-GEVLLSVETL 281
           Y+RLGGEDE   FFSE+D KNVVSW++ ISFYSS GD VK VDIF +IM GEV L +ETL
Sbjct: 181 YSRLGGEDEFVRFFSEIDCKNVVSWDILISFYSSVGDIVKAVDIFKQIMAGEVPLIIETL 240

Query: 282 TILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIP 341
           TIL+SAT   DS  LILG+NLHSLAIK+GLYD IL+TS LDMYAKFGEL+NSTRLF EIP
Sbjct: 241 TILISATKTSDSMCLILGENLHSLAIKTGLYDSILRTSLLDMYAKFGELDNSTRLFNEIP 300

Query: 342 RKSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKA 401
            +SIITWGAMMSS+ + GHFD AVEIF+QMQAAGLKPS+GILKHLIDAY HLG LQLG+ 
Sbjct: 301 NRSIITWGAMMSSFIQNGHFDEAVEIFSQMQAAGLKPSLGILKHLIDAYAHLGALQLGRG 360

Query: 402 IHCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAH 461
           IHCYLIR+ GLEI NT L TS++NMYVRCGS+ SA KCFDLI++KDVVAWTSMIEGYG+H
Sbjct: 361 IHCYLIRIYGLEICNTHLETSLMNMYVRCGSIASARKCFDLIIVKDVVAWTSMIEGYGSH 420

Query: 462 GLGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEH 521
           G G +ALNL+  MM EEV PN+VTFLSLLSACSHSGLVSEGC+IFYSMRSRFNI PDLEH
Sbjct: 421 GQGINALNLYHHMMSEEVAPNSVTFLSLLSACSHSGLVSEGCEIFYSMRSRFNIKPDLEH 480

Query: 522 YTCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELE 581
           YTCFVDLLSRSTRVREAFAIILRMTN  DGRIWGALMGACRVY DNKIA YAAHRLLELE
Sbjct: 481 YTCFVDLLSRSTRVREAFAIILRMTNLCDGRIWGALMGACRVYGDNKIAIYAAHRLLELE 540

Query: 582 PDNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           PDNVGYYTLLSN QA+VGQWH+VEKLRSVVYEKD VKKPGWSF+EL G +HGFVSGDR
Sbjct: 541 PDNVGYYTLLSNTQASVGQWHEVEKLRSVVYEKDFVKKPGWSFVELNGTLHGFVSGDR 598

BLAST of MC01g0212 vs. ExPASy TrEMBL
Match: A0A6J1HTH3 (pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467326 PE=4 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 469/598 (78.43%), Postives = 521/598 (87.12%), Query Frame = 0

Query: 42  MLWNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHC 101
           MLWNSIIKS  +SGLF SA++LYK MRE+GVEHDGFTFP++N ++MSI +DVVYAGMVHC
Sbjct: 1   MLWNSIIKSQFDSGLFQSAIMLYKNMREVGVEHDGFTFPILNHVVMSICVDVVYAGMVHC 60

Query: 102 VGIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVS 161
           VGIRMGFG+DLYFCNTMMEVY KC CL  AR VFDEMP+RDLVSWTSMIS YV  G +V 
Sbjct: 61  VGIRMGFGSDLYFCNTMMEVYAKCECLGHARKVFDEMPNRDLVSWTSMISAYVNSGVIVC 120

Query: 162 GLDLFEGMRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRM 221
            L+LFEGMRR LEPNSVT+M M+QACC T +L LGR +Q  V KNGLLFD+GLQN  LRM
Sbjct: 121 ALNLFEGMRRVLEPNSVTMMAMLQACCVTEDLVLGRLIQCLVVKNGLLFDVGLQNWFLRM 180

Query: 222 YTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIM-GEVLLSVETL 281
           Y+RLGGEDE    FSE+D KNVVSWN+ ISFY S GD VK VDIF +IM GEV L ++TL
Sbjct: 181 YSRLGGEDEFVRVFSEIDCKNVVSWNILISFYFSVGDIVKAVDIFKQIMSGEVPLIIDTL 240

Query: 282 TILVSATAAPDSEHLILGKNLHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIP 341
           TIL+SAT   +S  LILG+NLHSLAIK+GLYD IL+TS LDMYAK GEL+NSTRLF EIP
Sbjct: 241 TILISATKTSESMCLILGENLHSLAIKTGLYDSILRTSLLDMYAKIGELDNSTRLFNEIP 300

Query: 342 RKSIITWGAMMSSYSE-GHFDGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKA 401
            +SIITWGAMMSS+ + GHFD AV+IF+QMQAAGLKPS+GILKHLIDAY HLG LQLG+ 
Sbjct: 301 NRSIITWGAMMSSFIQNGHFDEAVDIFSQMQAAGLKPSLGILKHLIDAYAHLGALQLGRG 360

Query: 402 IHCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAH 461
           IHCYLIR++GLEI NT L TS++NMYVRCGS+ SA KCFDLI++KDVVAWTSMIEGYGAH
Sbjct: 361 IHCYLIRIHGLEICNTHLETSLMNMYVRCGSIASARKCFDLIIVKDVVAWTSMIEGYGAH 420

Query: 462 GLGFDALNLFLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEH 521
           G G +ALNL+  MM EEV PN+VTFLSLLSACSHSGLVSEGC+IFYSMRSRFNI PDLEH
Sbjct: 421 GQGINALNLYHHMMSEEVAPNSVTFLSLLSACSHSGLVSEGCEIFYSMRSRFNIKPDLEH 480

Query: 522 YTCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELE 581
           YTCFVDLLSRSTRVREAFAIILRMTN  DGRIWGALMGACRVY DNKIA YAAHRLLELE
Sbjct: 481 YTCFVDLLSRSTRVREAFAIILRMTNLCDGRIWGALMGACRVYGDNKIAIYAAHRLLELE 540

Query: 582 PDNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 637
           PDNVGYYTLLSN QA+VGQWH+VEKLRSVVYEK+LVKKPGWSFIEL G +HGFVSGDR
Sbjct: 541 PDNVGYYTLLSNTQASVGQWHEVEKLRSVVYEKNLVKKPGWSFIELNGTIHGFVSGDR 598

BLAST of MC01g0212 vs. ExPASy TrEMBL
Match: A0A540N4B4 (Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_008532 PE=4 SV=1)

HSP 1 Score: 744 bits (1920), Expect = 3.98e-262
Identity = 379/639 (59.31%), Postives = 474/639 (74.18%), Query Frame = 0

Query: 3   LSSGFDTVSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALL 62
           +S+G    S  SKL  LYA FDDL SAVSV  +  EP  MLWN ++KSHVE GL  SALL
Sbjct: 2   VSTGIQPSSHFSKLFTLYARFDDLDSAVSVFGSIREPNTMLWNLMMKSHVECGLVDSALL 61

Query: 63  LYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEVY 122
           LYKKMRELGV HD FTFP+VNR++M +  +V YAGMVHCV I+MGFG D+YF NTM+++Y
Sbjct: 62  LYKKMRELGVSHDCFTFPIVNRVVMLLGGEVGYAGMVHCVAIQMGFGMDMYFGNTMIDLY 121

Query: 123 GKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRRELEPNSVTIMV 182
            KCG +  AR +FDEM  RDLVSWTSMIS YV  G+V SGL LF  MR ELEPNSVT+++
Sbjct: 122 VKCGAIDHARKLFDEMCQRDLVSWTSMISGYVSEGNVPSGLSLFNEMRLELEPNSVTMLI 181

Query: 183 MVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKN 242
           M+Q CC T +   G Q   +V KNGLL+D  +QNS+LRMY +LG  +EV  FFSE+DR++
Sbjct: 182 MLQGCCGTESAICGSQFHGYVIKNGLLYDASVQNSILRMYAKLGTINEVEGFFSELDRRD 241

Query: 243 VVSWNLFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLIL--GKN 302
           VVSWN+ IS +SSRGD  KV ++FN + G+V+  VETLT+++SA     ++H IL  G++
Sbjct: 242 VVSWNICISIFSSRGDVAKVRELFNDMQGKVVPGVETLTLVISAL----TKHGILSQGES 301

Query: 303 LHSLAIKSGLYDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSE-GHF 362
           LH LAIK GL D +LQTS LD+YAK GEL  S RLF+EIP ++ ITWGAMM  + + G F
Sbjct: 302 LHCLAIKRGLCDHVLQTSLLDLYAKCGELGISDRLFREIPHRNSITWGAMMFGFIQNGWF 361

Query: 363 DGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYN--TQL 422
             AV +F +MQA G +P   IL+ L+DA+ +LG L+LGK IH Y+IR +  E     T L
Sbjct: 362 SEAVGLFREMQALGPEPRAEILRSLVDAFANLGALKLGKQIHGYIIRKSLYEDEESYTHL 421

Query: 423 GTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEV 482
            TSI+NMY+RCGSL +A  CFD +L+KD+V WTSMIEG G+HGLGF+AL LF  M+RE V
Sbjct: 422 ETSIINMYIRCGSLSAARVCFDRMLVKDIVTWTSMIEGCGSHGLGFEALKLFDLMIREGV 481

Query: 483 TPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAF 542
            PN+VTF+SLLSACSHSGLV+EGC +F SM+ +F I PDL+HYT  VDLL RS +++EA 
Sbjct: 482 RPNSVTFISLLSACSHSGLVTEGCDVFCSMKWKFGIEPDLDHYTSIVDLLGRSGKLKEAL 541

Query: 543 AIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVG 602
           A+I++M  F D RIWGAL+  CR+Y    +  YAA RLLELEPDN GYYTLLSN QA+VG
Sbjct: 542 AVIMKMMTFPDSRIWGALLSGCRIYSLRDVGEYAAQRLLELEPDNAGYYTLLSNTQASVG 601

Query: 603 QWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVSGD 636
           QW  VE+ R V+ E DL K PGWS+IE +G ++GFVSGD
Sbjct: 602 QWDGVEETRRVMSEMDLKKMPGWSWIEAEGRIYGFVSGD 636

BLAST of MC01g0212 vs. TAIR 10
Match: AT4G35130.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 6.7e-106
Identity = 206/628 (32.80%), Postives = 349/628 (55.57%), Query Frame = 0

Query: 14  SKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRELGVE 73
           ++ +R +A    +  A+ + +   + +  LWN +IK     GL++ A+  Y +M   GV+
Sbjct: 68  TRALRGFADSRLMEDALQLFDEMNKADAFLWNVMIKGFTSCGLYIEAVQFYSRMVFAGVK 127

Query: 74  HDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEVYGKCGCLVSARN 133
            D FT+P V + +  I   +     +H + I++GF +D+Y CN+++ +Y K GC   A  
Sbjct: 128 ADTFTYPFVIKSVAGIS-SLEEGKKIHAMVIKLGFVSDVYVCNSLISLYMKLGCAWDAEK 187

Query: 134 VFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRR-ELEPNSVTIMVMVQACCATGN 193
           VF+EMP RD+VSW SMIS Y+  GD  S L LF+ M +   +P+  + M  + AC    +
Sbjct: 188 VFEEMPERDIVSWNSMISGYLALGDGFSSLMLFKEMLKCGFKPDRFSTMSALGACSHVYS 247

Query: 194 LSLGRQLQSHVFKNGL-LFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWNLFIS 253
             +G+++  H  ++ +   D+ +  S+L MY++ G        F+ + ++N+V+WN+ I 
Sbjct: 248 PKMGKEIHCHAVRSRIETGDVMVMTSILDMYSKYGEVSYAERIFNGMIQRNIVAWNVMIG 307

Query: 254 FYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIKSG-L 313
            Y+  G        F K+  +  L  + +T   S    P S  ++ G+ +H  A++ G L
Sbjct: 308 CYARNGRVTDAFLCFQKMSEQNGLQPDVIT---SINLLPASA-ILEGRTIHGYAMRRGFL 367

Query: 314 YDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSY-SEGHFDGAVEIFNQM 373
              +L+T+ +DMY + G+L+++  +F  +  K++I+W +++++Y   G    A+E+F ++
Sbjct: 368 PHMVLETALIDMYGECGQLKSAEVIFDRMAEKNVISWNSIIAAYVQNGKNYSALELFQEL 427

Query: 374 QAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRCG 433
             + L P    +  ++ AY     L  G+ IH Y+++       NT +  S+++MY  CG
Sbjct: 428 WDSSLVPDSTTIASILPAYAESLSLSEGREIHAYIVKSRYWS--NTIILNSLVHMYAMCG 487

Query: 434 SLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFLSLLS 493
            L  A KCF+ IL+KDVV+W S+I  Y  HG G  ++ LF +M+   V PN  TF SLL+
Sbjct: 488 DLEDARKCFNHILLKDVVSWNSIIMAYAVHGFGRISVWLFSEMIASRVNPNKSTFASLLA 547

Query: 494 ACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRDG 553
           ACS SG+V EG + F SM+  + I+P +EHY C +DL+ R+     A   +  M      
Sbjct: 548 ACSISGMVDEGWEYFESMKREYGIDPGIEHYGCMLDLIGRTGNFSAAKRFLEEMPFVPTA 607

Query: 554 RIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSVV 613
           RIWG+L+ A R ++D  IA +AA ++ ++E DN G Y LL N  A  G+W DV +++ ++
Sbjct: 608 RIWGSLLNASRNHKDITIAEFAAEQIFKMEHDNTGCYVLLLNMYAEAGRWEDVNRIKLLM 667

Query: 614 YEKDLVKKPGWSFIELKGIVHGFVSGDR 638
             K + +    S +E KG  H F +GDR
Sbjct: 668 ESKGISRTSSRSTVEAKGKSHVFTNGDR 688

BLAST of MC01g0212 vs. TAIR 10
Match: AT4G18750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 363.2 bits (931), Expect = 4.2e-100
Identity = 218/628 (34.71%), Postives = 343/628 (54.62%), Query Frame = 0

Query: 14  SKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRELGVE 73
           SKL  +Y +  DL  A  V +     + + WN ++    +SG F  ++ L+KKM   GVE
Sbjct: 133 SKLSLMYTNCGDLKEASRVFDEVKIEKALFWNILMNELAKSGDFSGSIGLFKKMMSSGVE 192

Query: 74  HDGFTFPMVNRIIMSIQLDVVYAG-MVHCVGIRMGFGADLYFCNTMMEVYGKCGCLVSAR 133
            D +TF  V++   S++   V+ G  +H   ++ GFG      N+++  Y K   + SAR
Sbjct: 193 MDSYTFSCVSKSFSSLR--SVHGGEQLHGFILKSGFGERNSVGNSLVAFYLKNQRVDSAR 252

Query: 134 NVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMR-RELEPNSVTIMVMVQACCATG 193
            VFDEM  RD++SW S+I+ YV  G    GL +F  M    +E +  TI+ +   C  + 
Sbjct: 253 KVFDEMTERDVISWNSIINGYVSNGLAEKGLSVFVQMLVSGIEIDLATIVSVFAGCADSR 312

Query: 194 NLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWNLFIS 253
            +SLGR + S   K     +    N+LL MY++ G  D     F E+  ++VVS+   I+
Sbjct: 313 LISLGRAVHSIGVKACFSREDRFCNTLLDMYSKCGDLDSAKAVFREMSDRSVVSYTSMIA 372

Query: 254 FYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIKSGL- 313
            Y+  G   + V +F + M E  +S +  T+            L  GK +H    ++ L 
Sbjct: 373 GYAREGLAGEAVKLFEE-MEEEGISPDVYTVTAVLNCCARYRLLDEGKRVHEWIKENDLG 432

Query: 314 YDGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSEG-HFDGAVEIFN-Q 373
           +D  +  + +DMYAK G ++ +  +F E+  K II+W  ++  YS+  + + A+ +FN  
Sbjct: 433 FDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIISWNTIIGGYSKNCYANEALSLFNLL 492

Query: 374 MQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRC 433
           ++     P    +  ++ A   L     G+ IH Y++R NG    +  +  S+++MY +C
Sbjct: 493 LEEKRFSPDERTVACVLPACASLSAFDKGREIHGYIMR-NGY-FSDRHVANSLVDMYAKC 552

Query: 434 GSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFLSLL 493
           G+L+ A   FD I  KD+V+WT MI GYG HG G +A+ LF QM +  +  + ++F+SLL
Sbjct: 553 GALLLAHMLFDDIASKDLVSWTVMIAGYGMHGFGKEAIALFNQMRQAGIEADEISFVSLL 612

Query: 494 SACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRD 553
            ACSHSGLV EG + F  MR    I P +EHY C VD+L+R+  + +A+  I  M    D
Sbjct: 613 YACSHSGLVDEGWRFFNIMRHECKIEPTVEHYACIVDMLARTGDLIKAYRFIENMPIPPD 672

Query: 554 GRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSV 613
             IWGAL+  CR++ D K+A   A ++ ELEP+N GYY L++N  A   +W  V++LR  
Sbjct: 673 ATIWGALLCGCRIHHDVKLAEKVAEKVFELEPENTGYYVLMANIYAEAEKWEQVKRLRKR 732

Query: 614 VYEKDLVKKPGWSFIELKGIVHGFVSGD 637
           + ++ L K PG S+IE+KG V+ FV+GD
Sbjct: 733 IGQRGLRKNPGCSWIEIKGRVNIFVAGD 755

BLAST of MC01g0212 vs. TAIR 10
Match: AT1G15510.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 360.5 bits (924), Expect = 2.7e-99
Identity = 210/632 (33.23%), Postives = 337/632 (53.32%), Query Frame = 0

Query: 10  VSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSALLLYKKMRE 69
           V   +  + ++  F +L  A  V     E     WN ++  + + G F  A+ LY +M  
Sbjct: 129 VELGNAFLAMFVRFGNLVDAWYVFGKMSERNLFSWNVLVGGYAKQGYFDEAMCLYHRMLW 188

Query: 70  L-GVEHDGFTFPMVNRIIMSIQLDVVYAGMVHCVGIRMGFGADLYFCNTMMEVYGKCGCL 129
           + GV+ D +TFP V R    I  D+     VH   +R G+  D+   N ++ +Y KCG +
Sbjct: 189 VGGVKPDVYTFPCVLRTCGGIP-DLARGKEVHVHVVRYGYELDIDVVNALITMYVKCGDV 248

Query: 130 VSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMR-RELEPNSVTIMVMVQAC 189
            SAR +FD MP RD++SW +MIS Y   G    GL+LF  MR   ++P+ +T+  ++ AC
Sbjct: 249 KSARLLFDRMPRRDIISWNAMISGYFENGMCHEGLELFFAMRGLSVDPDLMTLTSVISAC 308

Query: 190 CATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFFSEVDRKNVVSWN 249
              G+  LGR + ++V   G   DI + NSL +MY   G   E    FS ++RK++VSW 
Sbjct: 309 ELLGDRRLGRDIHAYVITTGFAVDISVCNSLTQMYLNAGSWREAEKLFSRMERKDIVSWT 368

Query: 250 LFISFYSSRGDFVKVVDIFNKIMGEVLLSVETLTILVSATAAPDSEHLILGKNLHSLAIK 309
             IS Y       K +D + ++M +  +  + +T+    +A      L  G  LH LAIK
Sbjct: 369 TMISGYEYNFLPDKAIDTY-RMMDQDSVKPDEITVAAVLSACATLGDLDTGVELHKLAIK 428

Query: 310 SGLYD-GILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSSYSEGHFDGAVEIF 369
           + L    I+  + ++MY+K   ++ +  +F  IPRK++I+W ++++     +      IF
Sbjct: 429 ARLISYVIVANNLINMYSKCKCIDKALDIFHNIPRKNVISWTSIIAGLRLNNRCFEALIF 488

Query: 370 NQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLN-GLEIYNTQLGTSILNMY 429
            +     L+P+   L   + A   +G L  GK IH +++R   GL+ +   L  ++L+MY
Sbjct: 489 LRQMKMTLQPNAITLTAALAACARIGALMCGKEIHAHVLRTGVGLDDF---LPNALLDMY 548

Query: 430 VRCGSLVSAIKCFDLILIKDVVAWTSMIEGYGAHGLGFDALNLFLQMMREEVTPNNVTFL 489
           VRCG + +A   F+    KDV +W  ++ GY   G G   + LF +M++  V P+ +TF+
Sbjct: 549 VRCGRMNTAWSQFN-SQKKDVTSWNILLTGYSERGQGSMVVELFDRMVKSRVRPDEITFI 608

Query: 490 SLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLSRSTRVREAFAIILRMTN 549
           SLL  CS S +V +G   F  M   + + P+L+HY C VDLL R+  ++EA   I +M  
Sbjct: 609 SLLCGCSKSQMVRQGLMYFSKMED-YGVTPNLKHYACVVDLLGRAGELQEAHKFIQKMPV 668

Query: 550 FRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTLLSNAQATVGQWHDVEKL 609
             D  +WGAL+ ACR++    +   +A  + EL+  +VGYY LL N  A  G+W +V K+
Sbjct: 669 TPDPAVWGALLNACRIHHKIDLGELSAQHIFELDKKSVGYYILLCNLYADCGKWREVAKV 728

Query: 610 RSVVYEKDLVKKPGWSFIELKGIVHGFVSGDR 638
           R ++ E  L    G S++E+KG VH F+S D+
Sbjct: 729 RRMMKENGLTVDAGCSWVEVKGKVHAFLSDDK 753

BLAST of MC01g0212 vs. TAIR 10
Match: AT3G01580.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 3.9e-98
Identity = 210/602 (34.88%), Postives = 330/602 (54.82%), Query Frame = 0

Query: 44  WNSIIKSHVESGLFVSALLLYKKMRELGVEHDGFTFPMVNRIIMSIQLDVVYAGMVH-CV 103
           WN+++KS      +   L  +  M     + D FT P+  +    ++ +V Y  M+H  V
Sbjct: 28  WNTLLKSLSREKQWEEVLYHFSHMFRDEEKPDNFTLPVALKACGELR-EVNYGEMIHGFV 87

Query: 104 GIRMGFGADLYFCNTMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSG 163
              +  G+DLY  ++++ +Y KCG ++ A  +FDE+   D+V+W+SM+S +   G     
Sbjct: 88  KKDVTLGSDLYVGSSLIYMYIKCGRMIEALRMFDELEKPDIVTWSSMVSGFEKNGSPYQA 147

Query: 164 LDLFEG--MRRELEPNSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLR 223
           ++ F    M  ++ P+ VT++ +V AC    N  LGR +   V + G   D+ L NSLL 
Sbjct: 148 VEFFRRMVMASDVTPDRVTLITLVSACTKLSNSRLGRCVHGFVIRRGFSNDLSLVNSLLN 207

Query: 224 MYTRLGGEDEVGVFFSEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIM--GEVLLSVE 283
            Y +     E    F  +  K+V+SW+  I+ Y   G   + + +FN +M  G       
Sbjct: 208 CYAKSRAFKEAVNLFKMIAEKDVISWSTVIACYVQNGAAAEALLVFNDMMDDGTEPNVAT 267

Query: 284 TLTILVSATAAPDSEHLILGKNLHSLAIKSGLYDGI-LQTSFLDMYAKFGELENSTRLFK 343
            L +L +  AA D E    G+  H LAI+ GL   + + T+ +DMY K    E +  +F 
Sbjct: 268 VLCVLQACAAAHDLEQ---GRKTHELAIRKGLETEVKVSTALVDMYMKCFSPEEAYAVFS 327

Query: 344 EIPRKSIITWGAMMSSYS-EGHFDGAVEIFNQMQAA-GLKPSVGILKHLIDAYTHLGVLQ 403
            IPRK +++W A++S ++  G    ++E F+ M      +P   ++  ++ + + LG L+
Sbjct: 328 RIPRKDVVSWVALISGFTLNGMAHRSIEEFSIMLLENNTRPDAILMVKVLGSCSELGFLE 387

Query: 404 LGKAIHCYLIRLNGLEIYNTQLGTSILNMYVRCGSLVSAIKCFDLILIKDVVAWTSMIEG 463
             K  H Y+I+  G +  N  +G S++ +Y RCGSL +A K F+ I +KD V WTS+I G
Sbjct: 388 QAKCFHSYVIKY-GFD-SNPFIGASLVELYSRCGSLGNASKVFNGIALKDTVVWTSLITG 447

Query: 464 YGAHGLGFDALNLFLQMMR-EEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNIN 523
           YG HG G  AL  F  M++  EV PN VTFLS+LSACSH+GL+ EG +IF  M + + + 
Sbjct: 448 YGIHGKGTKALETFNHMVKSSEVKPNEVTFLSILSACSHAGLIHEGLRIFKLMVNDYRLA 507

Query: 524 PDLEHYTCFVDLLSRSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHR 583
           P+LEHY   VDLL R   +  A  I  RM      +I G L+GACR++++ ++A   A +
Sbjct: 508 PNLEHYAVLVDLLGRVGDLDTAIEITKRMPFSPTPQILGTLLGACRIHQNGEMAETVAKK 567

Query: 584 LLELEPDNVGYYTLLSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELKGIVHGFVS 637
           L ELE ++ GYY L+SN     G+W +VEKLR+ V ++ + K    S IE++  VH FV+
Sbjct: 568 LFELESNHAGYYMLMSNVYGVKGEWENVEKLRNSVKQRGIKKGLAESLIEIRRKVHRFVA 623

BLAST of MC01g0212 vs. TAIR 10
Match: AT5G39350.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 355.9 bits (912), Expect = 6.7e-98
Identity = 213/638 (33.39%), Postives = 350/638 (54.86%), Query Frame = 0

Query: 2   ILSSGFDTVSTASKLVRLYAHFDDLPSAVSVLNAFPEPEPMLWNSIIKSHVESGLFVSAL 61
           +++ G  +    S L   YA    +  A  +    P+   + +N +I+ +V  GL+  A+
Sbjct: 41  VITGGRVSGHILSTLSVTYALCGHITYARKLFEEMPQSSLLSYNIVIRMYVREGLYHDAI 100

Query: 62  LLYKKMRELGVE--HDGFTFPMVNRI---IMSIQLDVVYAGMVHCVGIRMGFGADLYFCN 121
            ++ +M   GV+   DG+T+P V +    + S++L +V  G +    +R  FG D Y  N
Sbjct: 101 SVFIRMVSEGVKCVPDGYTYPFVAKAAGELKSMKLGLVVHGRI----LRSWFGRDKYVQN 160

Query: 122 TMMEVYGKCGCLVSARNVFDEMPHRDLVSWTSMISVYVCRGDVVSGLDLFEGMRRE-LEP 181
            ++ +Y   G +  AR+VFD M +RD++SW +MIS Y   G +   L +F+ M  E ++ 
Sbjct: 161 ALLAMYMNFGKVEMARDVFDVMKNRDVISWNTMISGYYRNGYMNDALMMFDWMVNESVDL 220

Query: 182 NSVTIMVMVQACCATGNLSLGRQLQSHVFKNGLLFDIGLQNSLLRMYTRLGGEDEVGVFF 241
           +  TI+ M+  C    +L +GR +   V +  L   I ++N+L+ MY + G  DE    F
Sbjct: 221 DHATIVSMLPVCGHLKDLEMGRNVHKLVEEKRLGDKIEVKNALVNMYLKCGRMDEARFVF 280

Query: 242 SEVDRKNVVSWNLFISFYSSRGDFVKVVDIFNKIMGE-VLLSVETLTILVSATAAPDSEH 301
             ++R++V++W   I+ Y+  GD    +++   +  E V  +  T+  LVS     D+  
Sbjct: 281 DRMERRDVITWTCMINGYTEDGDVENALELCRLMQFEGVRPNAVTIASLVSVCG--DALK 340

Query: 302 LILGKNLHSLAIKSGLY-DGILQTSFLDMYAKFGELENSTRLFKEIPRKSIITWGAMMSS 361
           +  GK LH  A++  +Y D I++TS + MYAK   ++   R+F    +     W A+++ 
Sbjct: 341 VNDGKCLHGWAVRQQVYSDIIIETSLISMYAKCKRVDLCFRVFSGASKYHTGPWSAIIAG 400

Query: 362 YSEGHF-DGAVEIFNQMQAAGLKPSVGILKHLIDAYTHLGVLQLGKAIHCYLIRLNGLEI 421
             +      A+ +F +M+   ++P++  L  L+ AY  L  L+    IHCYL +   +  
Sbjct: 401 CVQNELVSDALGLFKRMRREDVEPNIATLNSLLPAYAALADLRQAMNIHCYLTKTGFMS- 460

Query: 422 YNTQLGTSILNMYVRCGSLVSAIKCFDLI----LIKDVVAWTSMIEGYGAHGLGFDALNL 481
            +    T ++++Y +CG+L SA K F+ I      KDVV W ++I GYG HG G +AL +
Sbjct: 461 -SLDAATGLVHVYSKCGTLESAHKIFNGIQEKHKSKDVVLWGALISGYGMHGDGHNALQV 520

Query: 482 FLQMMREEVTPNNVTFLSLLSACSHSGLVSEGCQIFYSMRSRFNINPDLEHYTCFVDLLS 541
           F++M+R  VTPN +TF S L+ACSHSGLV EG  +F  M   +       HYTC VDLL 
Sbjct: 521 FMEMVRSGVTPNEITFTSALNACSHSGLVEEGLTLFRFMLEHYKTLARSNHYTCIVDLLG 580

Query: 542 RSTRVREAFAIILRMTNFRDGRIWGALMGACRVYEDNKIANYAAHRLLELEPDNVGYYTL 601
           R+ R+ EA+ +I  +       +WGAL+ AC  +E+ ++   AA++L ELEP+N G Y L
Sbjct: 581 RAGRLDEAYNLITTIPFEPTSTVWGALLAACVTHENVQLGEMAANKLFELEPENTGNYVL 640

Query: 602 LSNAQATVGQWHDVEKLRSVVYEKDLVKKPGWSFIELK 627
           L+N  A +G+W D+EK+RS++    L KKPG S IE++
Sbjct: 641 LANIYAALGRWKDMEKVRSMMENVGLRKKPGHSTIEIR 670

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O496199.4e-10532.80Pentatricopeptide repeat-containing protein At4g35130, chloroplastic OS=Arabidop... [more]
Q9SN395.9e-9934.71Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
Q9M9E23.8e-9833.23Pentatricopeptide repeat-containing protein At1g15510, chloroplastic OS=Arabidop... [more]
Q9SS975.5e-9734.88Putative pentatricopeptide repeat-containing protein At3g01580 OS=Arabidopsis th... [more]
Q9FLZ99.4e-9733.39Pentatricopeptide repeat-containing protein At5g39350 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_022153922.10.099.16pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like [Momor... [more]
KAG7033612.10.079.94Pentatricopeptide repeat-containing protein, chloroplastic, partial [Cucurbita a... [more]
XP_023526509.10.079.62pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like [Cucur... [more]
XP_038883286.10.083.58pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like [Benin... [more]
XP_008457591.10.082.24PREDICTED: pentatricopeptide repeat-containing protein At4g35130, chloroplastic ... [more]
Match NameE-valueIdentityDescription
A0A6J1DKA10.099.16pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like OS=Mom... [more]
A0A1S3C6I70.082.24pentatricopeptide repeat-containing protein At4g35130, chloroplastic OS=Cucumis ... [more]
A0A6J1EYH50.078.93pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like OS=Cuc... [more]
A0A6J1HTH30.078.43pentatricopeptide repeat-containing protein At4g35130, chloroplastic-like OS=Cuc... [more]
A0A540N4B43.98e-26259.31Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_008532 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35130.16.7e-10632.80Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G18750.14.2e-10034.71Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G15510.12.7e-9933.23Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G01580.13.9e-9834.88Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT5G39350.16.7e-9833.39Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 44..72
e-value: 2.7E-4
score: 21.0
coord: 115..141
e-value: 4.0E-4
score: 20.4
coord: 345..373
e-value: 0.043
score: 14.1
coord: 244..270
e-value: 0.029
score: 14.6
coord: 317..343
e-value: 0.81
score: 10.1
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 444..492
e-value: 1.2E-9
score: 38.2
coord: 142..188
e-value: 1.9E-7
score: 31.1
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 144..171
e-value: 2.0E-4
score: 19.3
coord: 345..378
e-value: 1.7E-4
score: 19.5
coord: 482..516
e-value: 4.2E-4
score: 18.3
coord: 43..75
e-value: 5.5E-5
score: 21.1
coord: 447..480
e-value: 4.2E-5
score: 21.5
coord: 115..143
e-value: 9.8E-5
score: 20.3
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 445..479
score: 10.610596
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 343..376
score: 9.371969
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 40..74
score: 9.251395
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 111..145
score: 9.393891
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 196..293
e-value: 2.4E-7
score: 32.3
coord: 7..90
e-value: 7.0E-7
score: 30.7
coord: 97..195
e-value: 3.5E-21
score: 77.3
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 414..625
e-value: 9.1E-31
score: 109.4
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 294..411
e-value: 2.1E-13
score: 52.4
NoneNo IPR availablePANTHERPTHR47928REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 1..635
NoneNo IPR availablePANTHERPTHR47928:SF75REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 1..635

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0212.1MC01g0212.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding