MC01g0189 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0189
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionheat stress transcription factor B-3-like
LocationMC01: 8175887 .. 8178912 (-)
RNA-Seq ExpressionMC01g0189
SyntenyMC01g0189
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCATTTCTCTCTCTCTCGAGAGAAGAATTCTTGTTCTCTTCATTATATATCTACAAAACCCCTTTAATTTCTCCTTCTCTAAAATCCCACAAAATCCATAAATTCCATCCATATCCTCATCTCTCATATTCTACTTAATTGTTAACATGGAGGCCGGCGTGAGCAATGATCGGAAGGGGTCGCCGGCAGCGGCGGCGGAGGAGGAGAGTGTGATAATGAGAAAGTCAACGCCGCCGCCGTTTCTGGTGAAGACTTATAAAGCAGTGGAGGATCCGGCGACCGACGACGTGATATCGTGGAACGGCGACGGGACGGCGTTTGTGGTGTGGCAGACGGCGGAGTTCGCCAAGGATGTTCTTCCTACTCTCTTCAAGCACTGCAACTTCTCTAGCTTTGTTCGCCAGCTTAATACATACGTACGTATCTTTTAATTTTTTTGTTTTATGGTTAAATTATGGATTTCGTCCACTAATTTCAATTTGGTCCTCATAAGTTTCGATATGGTCCCTAGTTATTATTTAAACTACTTTTTGCTGCTCACTTGAAAATCTTATCTTTTATCTTCAGCTAAATTTATATAGTTGGTGGGATTTGACCAAAATATAAGGATCAAATGGAAAATTTTGACCGTCAACTCGAAACTTTTGAAAAAATTATTAAATTGACTCAAATCATAAAGGTTTAATTTGATGGGGTGTGATTGGAAATTCAAATTTTAACTTTTAAGGAAATTCTAAATGTCTTTACCAAATCTTGGGTTGAAATATGTTTTGTTTTTAGCTTGGGAATTGACGAGATGAAAGAGAAAATGCATGGGTTTAGATCTTGTTAAGTTTTTACTAAAAATGGAAAATTAAATAGGAAAAGGAAGAACAAGATATAGTTTGGCTAAGTTATGTTTTGTTTTTTGTTTTTAAATTAGTTTTTTTTTTTAATTTCTTTGGAATCGATTCCTGTAAGAGAGGCTCATTGTCTAAGTAACACAGTAACTAACATAGAGTTTAAATTCAAGACCTCATGATAAATGATACCACAAGGCCCAAGATCCTTGCCCTGTTGCGTTGGTTATCGGGAATGAATAAGTTTTGTTTTGAGTATGATATATATATTTTTTTAATTTTTGAGAATAGGAGAGTGGGTCCATGAATTTTACGATGGGGATTAGATTTTAAAGACAAAATATTATTTACATTTCTTTTCCTTGTCTCTTATTATTTTTTTTAAGATATTACCCATTCAAACAAAAAGATGTAAAACTAAGGTCGTGAGGAGGTAAACGTTTTGAAATAAACTCAATGGCCTTTTTTGTACCTTAGTTTACTAACAAAAATAAGGAACATGTAAATAACTAATTCTATAAAATTATTTAATATATTTTTACCAATAAATTAATTGAAAAGTTAACGAGGTAAAACAAAGTCAAACCGAACAATACTTCACCCTACAACAAATCTTGATTTTTATTATCGTAGTGATCAGTAGATAAAACACCCATTATATTCGTTAGGAGGGTAAATTTATTGAATTAGAAAAAAAATAAAATAAATAGTAGAAGGGAGAATAAAGTCTTTGTTTCTATCTTCAAAATAAATTTTGATTTTGACCTTTAGAGACTTAGTTTACTTATAAAGATTGATATTTCAAACACAACAAAATGGTTAAGTTGTACTTGTCCAACCTAACTTGATTTTGAATTTTTTCAATCAATAAATTTATTTTATTTGACATGTGGTTTAATTAATTGCATAAATTAAATAATAATCATATTTGTTACCCATACTAAATGGACGATAACAAAAGAAGGCAAAATCTTTAAAAGGCTTTAGTGAGCATATTAAGGCATACTCCTTATTTTTTTTTGTCCAATTTTGTCAATGAAGTTATTATTTATTTAAAATTTTAGTTTAGTCACGTAAAAGACATTTTAAAGTTAAGGATCTATTATATTTATTGTAACTCATTAACTTAAATTTTTGGAGTCTTAAAAAGGAAAATAATAATAATTAAATTGAAATTTTGAGTGGGCCAGAAAATTTTCACTATTACATTTACTTTCTATTTCAATCCTAGTATTTTTTTTTGATTGATTTTGTTAATAAGTTTATATTATTTATTTATTTTATTTTGTTTAGATATGTATTAGACACTTTTAAAGTTCACAGTATTTTTTTTGTTTGATTGATTTTGTTAATAAGTTTATATTATTTATTTATTTTATTTTGTTTAGGTATCTATTAGACACTTTTAAGGTTCAGAGATCTAAATTAACAAACTTATAATATTTAGCCATATCAAGTGCTTGATTCTCTTCTCAAATTCAAACTTTTTCGCAGGGGTTTAGAAAGGTGGCCACGACAAGATGGGAGTTTTGCAACGACAAATTTCAAAAGGGTGAGAAAGAAAAGCTCTGCGAAATACGAAGAAGGAAAGCATGGACGTCCAACAAACAACGCGTAGCCGAAGCATCAGAACATCACGAGCTCGATGAAGATCAAAGATCATCCTCGTCGAACTCGTCGTCTTCGGAGTACAATACTCTCGTGGACCAGAACAAGCGGTTGAAGGAAGAAAACGGGGCCTTAATCTCCGAGCTCGCGAGCATGAAAAACAAGTGTAGAGAGCTTCTTGATTTGGTGGCTAAGTGTGGAAAAAAGAGTTCAAAGGAAGAAGAGGTAGATGAGAGGCCAAAGCTATTTGGAGTGAGGTTGGAGGTTGAAGGAGAGAGGGAGAGGAAGAGAAAAAGAGGAGAAAACAATCAAGAGATCAATGAAACAGCAACTTTCTTGCTATCTCAATCATGCAAAATGAAGGTCAAAGAGGAGAAAGCAATTATTAGATTTTAAAAAGGCTACTAAAAAATCTAGTAGCTTATAGAAAGATTATGCTTGTTAGATGGCAAATAGTGCATTATGAAGAAGTATAGAAACATAATCTGTAAAAAGATTTTATTTGATTTTTTTGTTTTGTTTTTGTTATAATTTATAAAGACTAATAAAGGCTAAATGGCTAACATGTAAAATGGGC

mRNA sequence

GTCCATTTCTCTCTCTCTCGAGAGAAGAATTCTTGTTCTCTTCATTATATATCTACAAAACCCCTTTAATTTCTCCTTCTCTAAAATCCCACAAAATCCATAAATTCCATCCATATCCTCATCTCTCATATTCTACTTAATTGTTAACATGGAGGCCGGCGTGAGCAATGATCGGAAGGGGTCGCCGGCAGCGGCGGCGGAGGAGGAGAGTGTGATAATGAGAAAGTCAACGCCGCCGCCGTTTCTGGTGAAGACTTATAAAGCAGTGGAGGATCCGGCGACCGACGACGTGATATCGTGGAACGGCGACGGGACGGCGTTTGTGGTGTGGCAGACGGCGGAGTTCGCCAAGGATGTTCTTCCTACTCTCTTCAAGCACTGCAACTTCTCTAGCTTTGTTCGCCAGCTTAATACATACGGGTTTAGAAAGGTGGCCACGACAAGATGGGAGTTTTGCAACGACAAATTTCAAAAGGGTGAGAAAGAAAAGCTCTGCGAAATACGAAGAAGGAAAGCATGGACGTCCAACAAACAACGCGTAGCCGAAGCATCAGAACATCACGAGCTCGATGAAGATCAAAGATCATCCTCGTCGAACTCGTCGTCTTCGGAGTACAATACTCTCGTGGACCAGAACAAGCGGTTGAAGGAAGAAAACGGGGCCTTAATCTCCGAGCTCGCGAGCATGAAAAACAAGTGTAGAGAGCTTCTTGATTTGGTGGCTAAGTGTGGAAAAAAGAGTTCAAAGGAAGAAGAGGTAGATGAGAGGCCAAAGCTATTTGGAGTGAGGTTGGAGGTTGAAGGAGAGAGGGAGAGGAAGAGAAAAAGAGGAGAAAACAATCAAGAGATCAATGAAACAGCAACTTTCTTGCTATCTCAATCATGCAAAATGAAGGTCAAAGAGGAGAAAGCAATTATTAGATTTTAAAAAGGCTACTAAAAAATCTAGTAGCTTATAGAAAGATTATGCTTGTTAGATGGCAAATAGTGCATTATGAAGAAGTATAGAAACATAATCTGTAAAAAGATTTTATTTGATTTTTTTGTTTTGTTTTTGTTATAATTTATAAAGACTAATAAAGGCTAAATGGCTAACATGTAAAATGGGC

Coding sequence (CDS)

ATGGAGGCCGGCGTGAGCAATGATCGGAAGGGGTCGCCGGCAGCGGCGGCGGAGGAGGAGAGTGTGATAATGAGAAAGTCAACGCCGCCGCCGTTTCTGGTGAAGACTTATAAAGCAGTGGAGGATCCGGCGACCGACGACGTGATATCGTGGAACGGCGACGGGACGGCGTTTGTGGTGTGGCAGACGGCGGAGTTCGCCAAGGATGTTCTTCCTACTCTCTTCAAGCACTGCAACTTCTCTAGCTTTGTTCGCCAGCTTAATACATACGGGTTTAGAAAGGTGGCCACGACAAGATGGGAGTTTTGCAACGACAAATTTCAAAAGGGTGAGAAAGAAAAGCTCTGCGAAATACGAAGAAGGAAAGCATGGACGTCCAACAAACAACGCGTAGCCGAAGCATCAGAACATCACGAGCTCGATGAAGATCAAAGATCATCCTCGTCGAACTCGTCGTCTTCGGAGTACAATACTCTCGTGGACCAGAACAAGCGGTTGAAGGAAGAAAACGGGGCCTTAATCTCCGAGCTCGCGAGCATGAAAAACAAGTGTAGAGAGCTTCTTGATTTGGTGGCTAAGTGTGGAAAAAAGAGTTCAAAGGAAGAAGAGGTAGATGAGAGGCCAAAGCTATTTGGAGTGAGGTTGGAGGTTGAAGGAGAGAGGGAGAGGAAGAGAAAAAGAGGAGAAAACAATCAAGAGATCAATGAAACAGCAACTTTCTTGCTATCTCAATCATGCAAAATGAAGGTCAAAGAGGAGAAAGCAATTATTAGATTTTAA

Protein sequence

MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATFLLSQSCKMKVKEEKAIIRF
Homology
BLAST of MC01g0189 vs. ExPASy Swiss-Prot
Match: O22230 (Heat stress transcription factor B-3 OS=Arabidopsis thaliana OX=3702 GN=HSFB3 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.0e-49
Identity = 116/212 (54.72%), Postives = 140/212 (66.04%), Query Frame = 0

Query: 29  PPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLN 88
           PPPFLVKTYK VEDP TD VISWN  GT FVVWQ AEFA+D+LPTLFKHCNFSSFVRQLN
Sbjct: 38  PPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLN 97

Query: 89  TYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKA--WTSNK-----------------Q 148
           TYGFRKV T RWEF N+ F+KG++E +  IRRRK+  W+ NK                 Q
Sbjct: 98  TYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQ 157

Query: 149 RVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASMKNKCRELLD 208
           R+    +HH   EDQ+SS++ SSS  Y  L+D+NK LK EN  L  EL   K KC++L++
Sbjct: 158 RI--GIDHHH--EDQQSSAT-SSSFVYTALLDENKCLKNENELLSCELGKTKKKCKQLME 217

Query: 209 LVAKC-----GKKSSKEEEVDERPKLFGVRLE 217
           LV +            ++E DE  KLFGV+LE
Sbjct: 218 LVERYRGEDEDATDESDDEEDEGLKLFGVKLE 244

BLAST of MC01g0189 vs. ExPASy Swiss-Prot
Match: P22335 (Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 2.1e-39
Identity = 93/187 (49.73%), Postives = 120/187 (64.17%), Query Frame = 0

Query: 25  RKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFV 84
           +++ P PFL+KTY+ V+D ATDDVISWN  GT FVVW+TAEFAKD+LP  FKH NFSSFV
Sbjct: 3   QRTAPAPFLLKTYQLVDDAATDDVISWNEIGTTFVVWKTAEFAKDLLPKYFKHNNFSSFV 62

Query: 85  RQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAWTS----NKQRVAEASEHHEL 144
           RQLNTYGFRK+   +WEF N+ F++G+KE L  IRRRK  TS     K   A AS   + 
Sbjct: 63  RQLNTYGFRKIVPDKWEFANENFKRGQKELLTAIRRRKTVTSTPAGGKSVAAGASASPDN 122

Query: 145 DEDQRSSSSNSSS--------------SEYNTLVDQNKRLKEENGALISELASMKNKCRE 194
             D   SSS SS               S++  L D+N++LK++N  L SEL   K +C E
Sbjct: 123 SGDDIGSSSTSSPDSKNPGSVDTPGKLSQFTDLSDENEKLKKDNQMLSSELVQAKKQCNE 182

BLAST of MC01g0189 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 4.5e-37
Identity = 95/216 (43.98%), Postives = 121/216 (56.02%), Query Frame = 0

Query: 13  PAAAAEEESV---IMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKD 72
           PA AAE   V     +++ P PFL KTY+ V+DPA DDVISWN DG+ FVVW+ AEFA+D
Sbjct: 27  PAPAAEAAGVGVGQQQRTVPTPFLTKTYQLVDDPAVDDVISWNDDGSTFVVWRPAEFARD 86

Query: 73  VLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAW----- 132
           +LP  FKH NFSSFVRQLNTYGFRK+   RWEF ND F++GE+  LCEI RRK       
Sbjct: 87  LLPKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTPPAPA 146

Query: 133 -------------------------TSNKQRVAEASEHHE--LDEDQRSSSSNSSSSEYN 192
                                     S +++V  +S   E  L   Q  S S S      
Sbjct: 147 ATTAAVAAAIPMALPVTTTRDGSPVLSGEEQVISSSSSPEPPLVLPQAPSGSGSGGVASG 206

Query: 193 TLVDQNKRLKEENGALISELASMKNKCRELLDLVAK 194
            + D+N+RL+ EN  L  EL+ M+  C  +L L++K
Sbjct: 207 DVGDENERLRRENAQLARELSQMRKLCNNILLLMSK 242

BLAST of MC01g0189 vs. ExPASy Swiss-Prot
Match: Q96320 (Heat stress transcription factor B-1 OS=Arabidopsis thaliana OX=3702 GN=HSFB1 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 2.2e-36
Identity = 114/256 (44.53%), Postives = 144/256 (56.25%), Query Frame = 0

Query: 25  RKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFV 84
           ++S P PFL KTY+ V+D +TDDV+SWN +GTAFVVW+TAEFAKD+LP  FKH NFSSF+
Sbjct: 8   QRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSFI 67

Query: 85  RQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRK---AWTSNK-QRVAEASEHHEL 144
           RQLNTYGFRK    +WEF ND F++G ++ L +IRRRK   A T+ K   V   SE +  
Sbjct: 68  RQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNSG 127

Query: 145 DEDQRSSSSNSS-------SSEYNTLVD---QNKRLKEENGALISELASMKNKCREL--- 204
             D   SSS SS        S  N + D   +N++LK EN  L SELA+ K +  EL   
Sbjct: 128 GGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELAAAKKQRDELVTF 187

Query: 205 -----------LDLVAKCGK-----------------KSSKEEEVDERPKLFGVRLEVEG 236
                      +D + K GK                     EE V E  KLFGV L  +G
Sbjct: 188 LTGHLKVRPEQIDKMIKGGKFKPVESDEESECEGCDGGGGAEEGVGEGLKLFGVWL--KG 247

BLAST of MC01g0189 vs. ExPASy Swiss-Prot
Match: Q652B0 (Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2C PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-35
Identity = 102/247 (41.30%), Postives = 128/247 (51.82%), Query Frame = 0

Query: 3   AGVSNDRKGSPAAA----AEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAF 62
           AG ++   G P  A    A  E++  ++S P PFL KTY+ VEDPA DDVISWN DG+ F
Sbjct: 6   AGEADAGGGEPPPAAVMTAAAEALAGQRSLPTPFLTKTYQLVEDPAVDDVISWNEDGSTF 65

Query: 63  VVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEI 122
           VVW+ AEFA+D+LP  FKH NFSSFVRQLNTYGFRK+   RWEF ND F++GEK  LC+I
Sbjct: 66  VVWRPAEFARDLLPKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGEKRLLCDI 125

Query: 123 RRRK---------------------AWTSNKQRVAEA------------SEHHELDEDQR 182
            RRK                     A  S    VA A            S  H  +E   
Sbjct: 126 HRRKVVAAAAAAPPPPSPGMATAAAAVASGAVTVAAAPIPMALPVTRAGSPAHSSEEQVL 185

Query: 183 SSSSNS-------------------SSSEYNTLVDQNKRLKEENGALISELASMKNKCRE 194
           SS+S S                    S+    + ++N+RL+ EN  L  EL  MK  C  
Sbjct: 186 SSNSGSGEEHRQASGSGSAPGGGGGGSASGGDMGEENERLRRENARLTRELGHMKKLCNN 245

BLAST of MC01g0189 vs. NCBI nr
Match: XP_022152241.1 (heat stress transcription factor B-3-like [Momordica charantia])

HSP 1 Score: 493 bits (1270), Expect = 1.53e-175
Identity = 258/259 (99.61%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVV 60
           MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVV
Sbjct: 1   MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVV 60

Query: 61  WQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRR 120
           WQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCN+KFQKGEKEKLCEIRR
Sbjct: 61  WQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNNKFQKGEKEKLCEIRR 120

Query: 121 RKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASM 180
           RKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASM
Sbjct: 121 RKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASM 180

Query: 181 KNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATF 240
           KNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATF
Sbjct: 181 KNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATF 240

Query: 241 LLSQSCKMKVKEEKAIIRF 259
           LLSQSCKMKVKEEKAIIRF
Sbjct: 241 LLSQSCKMKVKEEKAIIRF 259

BLAST of MC01g0189 vs. NCBI nr
Match: XP_022967894.1 (heat stress transcription factor B-3 [Cucurbita maxima])

HSP 1 Score: 337 bits (865), Expect = 3.63e-114
Identity = 192/252 (76.19%), Postives = 206/252 (81.75%), Query Frame = 0

Query: 1   MEAGVSN---DRKGS--PAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDG 60
           MEAGVSN   D+KG   P+     ES+I +KSTPPPFLVKTY  VEDPATD VISWNG+G
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVESMI-KKSTPPPFLVKTYTVVEDPATDKVISWNGEG 60

Query: 61  TAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKL 120
           TAFVVWQTAEFAKDVLP LFKHCNFSSFVRQLNTYGFRKV TTRWEFCNDKF+KGEKEKL
Sbjct: 61  TAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKL 120

Query: 121 CEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALIS 180
           CEIRRRKAWT+ +Q   +    HE+DEDQRSSSSNSSSS+YNTL+D+NKRLK+ENGAL S
Sbjct: 121 CEIRRRKAWTNKQQHEVQ----HEVDEDQRSSSSNSSSSQYNTLMDENKRLKKENGALSS 180

Query: 181 ELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEIN 240
           ELASMK KCRELLDLVAK G  S KEEE DERPKLFGVRLEVEGER RK KR E      
Sbjct: 181 ELASMKEKCRELLDLVAKYGNGSRKEEE-DERPKLFGVRLEVEGERRRKTKRAEET---- 239

Query: 241 ETATFLLSQSCK 247
              T L SQSCK
Sbjct: 241 ---TLLPSQSCK 239

BLAST of MC01g0189 vs. NCBI nr
Match: XP_038883399.1 (heat stress transcription factor B-3-like [Benincasa hispida])

HSP 1 Score: 336 bits (862), Expect = 9.35e-114
Identity = 192/253 (75.89%), Postives = 210/253 (83.00%), Query Frame = 0

Query: 1   MEAGVSN------DRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGD 60
           MEAGVSN      D KG PA    E   I+RKSTP PFLVKTY  VEDPATD+VISWNG+
Sbjct: 1   MEAGVSNNNNNKNDEKGLPA----ESDQIIRKSTPAPFLVKTYTVVEDPATDEVISWNGN 60

Query: 61  GTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEK 120
           GTAFVVWQTAEFAKDVLP LFKHCNFSSFVRQLNTYGFRKV TTRWEF NDKFQKGEKEK
Sbjct: 61  GTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFYNDKFQKGEKEK 120

Query: 121 LCEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALI 180
           LCEIRRRK WT+ +Q+        E+DEDQRS+SSNSSSS+YNTL+D+NKRLK+ENGAL 
Sbjct: 121 LCEIRRRKTWTNKQQQ--------EVDEDQRSTSSNSSSSQYNTLMDENKRLKKENGALS 180

Query: 181 SELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEI 240
           SELA+MK+KC+EL DLVAK  +  SK+EE DERPKLFGVRLE EGERERKRKR E   EI
Sbjct: 181 SELATMKSKCKELFDLVAKY-QNVSKKEEKDERPKLFGVRLE-EGERERKRKRAE---EI 236

Query: 241 NETATFLLSQSCK 247
           +ETATFLLSQSCK
Sbjct: 241 SETATFLLSQSCK 236

BLAST of MC01g0189 vs. NCBI nr
Match: XP_022928625.1 (heat stress transcription factor B-3 [Cucurbita moschata])

HSP 1 Score: 336 bits (861), Expect = 1.47e-113
Identity = 193/254 (75.98%), Postives = 207/254 (81.50%), Query Frame = 0

Query: 1   MEAGVSN---DRKG----SPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNG 60
           MEAGVSN   D+KG    SP    E    +M+KSTPPPFLVKTY  VEDPATD VISWN 
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVES---MMKKSTPPPFLVKTYTVVEDPATDKVISWNS 60

Query: 61  DGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKE 120
           +GTAFVVWQTAEFAKDVLP LFKHCNFSSFVRQLNTYGFRKV TTRWEFCNDKF+KGEKE
Sbjct: 61  EGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKE 120

Query: 121 KLCEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGAL 180
           KLCEIRRRKAWT+ +Q   +    HE+DEDQRSSSSNSSSS+YNTL+D+NKRLK+ENGAL
Sbjct: 121 KLCEIRRRKAWTNKQQHEVQ----HEVDEDQRSSSSNSSSSQYNTLMDENKRLKKENGAL 180

Query: 181 ISELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQE 240
            SELASMK KCRELLDLVAK G  S KE+E DERPKLFGVRLEVEGER RK KR E    
Sbjct: 181 SSELASMKGKCRELLDLVAKYGNGSRKEDE-DERPKLFGVRLEVEGERRRKTKRAE---- 239

Query: 241 INETATFLLSQSCK 247
             ETA FL SQSCK
Sbjct: 241 --ETA-FLPSQSCK 239

BLAST of MC01g0189 vs. NCBI nr
Match: KAG7033512.1 (Heat stress transcription factor B-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 335 bits (859), Expect = 2.97e-113
Identity = 193/254 (75.98%), Postives = 206/254 (81.10%), Query Frame = 0

Query: 1   MEAGVSN---DRKG----SPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNG 60
           MEAGVSN   D+KG    SP    E    +M+KSTPPPFLVKTY  VEDPATD VISWNG
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVES---MMKKSTPPPFLVKTYTVVEDPATDKVISWNG 60

Query: 61  DGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKE 120
           +GTAFVVWQTAEFAKDVLP LFKHCNFSSFVRQLNTYGFRKV TTRWEFCNDKF+KGEKE
Sbjct: 61  EGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKE 120

Query: 121 KLCEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGAL 180
           KLCEIRRRKAWT+ +Q   +    HE+DEDQRSSSSNSSSS YNTL+D+NKRLK+ENGAL
Sbjct: 121 KLCEIRRRKAWTNKQQHEVQ----HEVDEDQRSSSSNSSSSHYNTLMDENKRLKKENGAL 180

Query: 181 ISELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQE 240
             ELASMK KCRELLDLVAK G  S KE+E DERPKLFGVRLEVEGER RK KR E    
Sbjct: 181 SLELASMKGKCRELLDLVAKYGNGSRKEDE-DERPKLFGVRLEVEGERRRKTKRAE---- 239

Query: 241 INETATFLLSQSCK 247
             ETA FL SQSCK
Sbjct: 241 --ETA-FLPSQSCK 239

BLAST of MC01g0189 vs. ExPASy TrEMBL
Match: A0A6J1DH46 (heat stress transcription factor B-3-like OS=Momordica charantia OX=3673 GN=LOC111020012 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 7.41e-176
Identity = 258/259 (99.61%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVV 60
           MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVV
Sbjct: 1   MEAGVSNDRKGSPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVV 60

Query: 61  WQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRR 120
           WQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCN+KFQKGEKEKLCEIRR
Sbjct: 61  WQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNNKFQKGEKEKLCEIRR 120

Query: 121 RKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASM 180
           RKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASM
Sbjct: 121 RKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASM 180

Query: 181 KNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATF 240
           KNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATF
Sbjct: 181 KNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEINETATF 240

Query: 241 LLSQSCKMKVKEEKAIIRF 259
           LLSQSCKMKVKEEKAIIRF
Sbjct: 241 LLSQSCKMKVKEEKAIIRF 259

BLAST of MC01g0189 vs. ExPASy TrEMBL
Match: A0A6J1HY14 (heat stress transcription factor B-3 OS=Cucurbita maxima OX=3661 GN=LOC111467268 PE=3 SV=1)

HSP 1 Score: 337 bits (865), Expect = 1.76e-114
Identity = 192/252 (76.19%), Postives = 206/252 (81.75%), Query Frame = 0

Query: 1   MEAGVSN---DRKGS--PAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNGDG 60
           MEAGVSN   D+KG   P+     ES+I +KSTPPPFLVKTY  VEDPATD VISWNG+G
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVESMI-KKSTPPPFLVKTYTVVEDPATDKVISWNGEG 60

Query: 61  TAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKEKL 120
           TAFVVWQTAEFAKDVLP LFKHCNFSSFVRQLNTYGFRKV TTRWEFCNDKF+KGEKEKL
Sbjct: 61  TAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKEKL 120

Query: 121 CEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALIS 180
           CEIRRRKAWT+ +Q   +    HE+DEDQRSSSSNSSSS+YNTL+D+NKRLK+ENGAL S
Sbjct: 121 CEIRRRKAWTNKQQHEVQ----HEVDEDQRSSSSNSSSSQYNTLMDENKRLKKENGALSS 180

Query: 181 ELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQEIN 240
           ELASMK KCRELLDLVAK G  S KEEE DERPKLFGVRLEVEGER RK KR E      
Sbjct: 181 ELASMKEKCRELLDLVAKYGNGSRKEEE-DERPKLFGVRLEVEGERRRKTKRAEET---- 239

Query: 241 ETATFLLSQSCK 247
              T L SQSCK
Sbjct: 241 ---TLLPSQSCK 239

BLAST of MC01g0189 vs. ExPASy TrEMBL
Match: A0A6J1ES59 (heat stress transcription factor B-3 OS=Cucurbita moschata OX=3662 GN=LOC111435477 PE=3 SV=1)

HSP 1 Score: 336 bits (861), Expect = 7.13e-114
Identity = 193/254 (75.98%), Postives = 207/254 (81.50%), Query Frame = 0

Query: 1   MEAGVSN---DRKG----SPAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISWNG 60
           MEAGVSN   D+KG    SP    E    +M+KSTPPPFLVKTY  VEDPATD VISWN 
Sbjct: 1   MEAGVSNNNGDQKGLRLPSPPRTVES---MMKKSTPPPFLVKTYTVVEDPATDKVISWNS 60

Query: 61  DGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGEKE 120
           +GTAFVVWQTAEFAKDVLP LFKHCNFSSFVRQLNTYGFRKV TTRWEFCNDKF+KGEKE
Sbjct: 61  EGTAFVVWQTAEFAKDVLPKLFKHCNFSSFVRQLNTYGFRKVTTTRWEFCNDKFRKGEKE 120

Query: 121 KLCEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGAL 180
           KLCEIRRRKAWT+ +Q   +    HE+DEDQRSSSSNSSSS+YNTL+D+NKRLK+ENGAL
Sbjct: 121 KLCEIRRRKAWTNKQQHEVQ----HEVDEDQRSSSSNSSSSQYNTLMDENKRLKKENGAL 180

Query: 181 ISELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENNQE 240
            SELASMK KCRELLDLVAK G  S KE+E DERPKLFGVRLEVEGER RK KR E    
Sbjct: 181 SSELASMKGKCRELLDLVAKYGNGSRKEDE-DERPKLFGVRLEVEGERRRKTKRAE---- 239

Query: 241 INETATFLLSQSCK 247
             ETA FL SQSCK
Sbjct: 241 --ETA-FLPSQSCK 239

BLAST of MC01g0189 vs. ExPASy TrEMBL
Match: A0A5D3CGR4 (Heat stress transcription factor B-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold73G00700 PE=3 SV=1)

HSP 1 Score: 313 bits (801), Expect = 9.83e-105
Identity = 182/256 (71.09%), Postives = 201/256 (78.52%), Query Frame = 0

Query: 1   MEAGVSNDRKGS---------PAAAAEEESVIMRKSTPPPFLVKTYKAVEDPATDDVISW 60
           MEAGVS++             P   AE E + MRKST  PFL+KTY+ VEDP TDDVISW
Sbjct: 1   MEAGVSDNNNNRNEEKKLESWPPWTAESE-IRMRKSTTAPFLMKTYRMVEDPGTDDVISW 60

Query: 61  NGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLNTYGFRKVATTRWEFCNDKFQKGE 120
           N DGTAFVVWQTAEFAKD+LP LFKH NFSSFVRQLNTYGFRKV T RWEFCN+KFQKGE
Sbjct: 61  NSDGTAFVVWQTAEFAKDILPKLFKHSNFSSFVRQLNTYGFRKVTTKRWEFCNEKFQKGE 120

Query: 121 KEKLCEIRRRKAWTSNKQRVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENG 180
           K+KLCEI+RRK W SNKQ+       H +DEDQRS+SSNSSSS+YNTL+D+NKRLK+ENG
Sbjct: 121 KKKLCEIQRRKKW-SNKQQ-------HGVDEDQRSTSSNSSSSQYNTLMDENKRLKKENG 180

Query: 181 ALISELASMKNKCRELLDLVAKCGKKSSKEEEVDERPKLFGVRLEVEGERERKRKRGENN 240
           AL SELASMKNKC+EL DLVA     S K+E  DERPKLFGVRL  EGERERKRKRGE  
Sbjct: 181 ALSSELASMKNKCKELFDLVATYENISKKDE--DERPKLFGVRL--EGERERKRKRGE-- 240

Query: 241 QEINETATFLLSQSCK 247
            EI+ET T LLSQSCK
Sbjct: 241 -EISETTTILLSQSCK 240

BLAST of MC01g0189 vs. ExPASy TrEMBL
Match: A0A314YHN6 (Heat stress transcription factor B-3 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_36161 PE=3 SV=1)

HSP 1 Score: 307 bits (786), Expect = 2.15e-102
Identity = 166/235 (70.64%), Postives = 195/235 (82.98%), Query Frame = 0

Query: 24  MRKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSF 83
           +RKS+PPPFL+KTY  VEDP TDDVISWN DG+AFVVWQ AEFA+D+LPTLFKH NFSSF
Sbjct: 14  VRKSSPPPFLLKTYMLVEDPTTDDVISWNHDGSAFVVWQPAEFARDLLPTLFKHSNFSSF 73

Query: 84  VRQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAWTSNKQRV--------AEAS 143
           VRQLNTYGFRKV+T+RWEFCNDKF+KGEK++LC+IRRRKAW S +Q +        A+A 
Sbjct: 74  VRQLNTYGFRKVSTSRWEFCNDKFRKGEKDQLCDIRRRKAWASKQQPINNIALNQAAQAM 133

Query: 144 EHH-ELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASMKNKCRELLDLVAKC 203
            +H E DEDQRS+SS SSSS+Y++LVD+NKRLK+ENG L SEL SMK KC+ELLDLVAKC
Sbjct: 134 PNHDEFDEDQRSNSSTSSSSDYSSLVDENKRLKQENGVLSSELTSMKRKCKELLDLVAKC 193

Query: 204 GKKSSKEEEVDER--PKLFGVRLEVEGERERKRKRGENNQEINETATFLLSQSCK 247
           G  + KEEE +    PKLFGVRLEVEGERERKRKR E    I+E+A+ LLSQ+CK
Sbjct: 194 GDSTEKEEEENSERVPKLFGVRLEVEGERERKRKRAE----ISESASILLSQACK 244

BLAST of MC01g0189 vs. TAIR 10
Match: AT2G41690.1 (heat shock transcription factor B3 )

HSP 1 Score: 198.4 bits (503), Expect = 7.3e-51
Identity = 116/212 (54.72%), Postives = 140/212 (66.04%), Query Frame = 0

Query: 29  PPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVRQLN 88
           PPPFLVKTYK VEDP TD VISWN  GT FVVWQ AEFA+D+LPTLFKHCNFSSFVRQLN
Sbjct: 38  PPPFLVKTYKVVEDPTTDGVISWNEYGTGFVVWQPAEFARDLLPTLFKHCNFSSFVRQLN 97

Query: 89  TYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKA--WTSNK-----------------Q 148
           TYGFRKV T RWEF N+ F+KG++E +  IRRRK+  W+ NK                 Q
Sbjct: 98  TYGFRKVTTIRWEFSNEMFRKGQRELMSNIRRRKSQHWSHNKSNHQVVPTTTMVNQEGHQ 157

Query: 149 RVAEASEHHELDEDQRSSSSNSSSSEYNTLVDQNKRLKEENGALISELASMKNKCRELLD 208
           R+    +HH   EDQ+SS++ SSS  Y  L+D+NK LK EN  L  EL   K KC++L++
Sbjct: 158 RI--GIDHHH--EDQQSSAT-SSSFVYTALLDENKCLKNENELLSCELGKTKKKCKQLME 217

Query: 209 LVAKC-----GKKSSKEEEVDERPKLFGVRLE 217
           LV +            ++E DE  KLFGV+LE
Sbjct: 218 LVERYRGEDEDATDESDDEEDEGLKLFGVKLE 244

BLAST of MC01g0189 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 154.1 bits (388), Expect = 1.6e-37
Identity = 114/256 (44.53%), Postives = 144/256 (56.25%), Query Frame = 0

Query: 25  RKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFV 84
           ++S P PFL KTY+ V+D +TDDV+SWN +GTAFVVW+TAEFAKD+LP  FKH NFSSF+
Sbjct: 8   QRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSFI 67

Query: 85  RQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRK---AWTSNK-QRVAEASEHHEL 144
           RQLNTYGFRK    +WEF ND F++G ++ L +IRRRK   A T+ K   V   SE +  
Sbjct: 68  RQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNSG 127

Query: 145 DEDQRSSSSNSS-------SSEYNTLVD---QNKRLKEENGALISELASMKNKCREL--- 204
             D   SSS SS        S  N + D   +N++LK EN  L SELA+ K +  EL   
Sbjct: 128 GGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENNNLSSELAAAKKQRDELVTF 187

Query: 205 -----------LDLVAKCGK-----------------KSSKEEEVDERPKLFGVRLEVEG 236
                      +D + K GK                     EE V E  KLFGV L  +G
Sbjct: 188 LTGHLKVRPEQIDKMIKGGKFKPVESDEESECEGCDGGGGAEEGVGEGLKLFGVWL--KG 247

BLAST of MC01g0189 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 144.1 bits (362), Expect = 1.6e-34
Identity = 101/259 (39.00%), Postives = 136/259 (52.51%), Query Frame = 0

Query: 25  RKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFV 84
           ++S P PFL KT+  VED + DDVISWN DG++F+VW   +FAKD+LP  FKH NFSSFV
Sbjct: 17  QRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSFV 76

Query: 85  RQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAWTSNKQRVAEASEHHEL---- 144
           RQLNTYGF+KV   RWEF ND F++GEK  L EI+RRK  T+++  VA +SE        
Sbjct: 77  RQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQTMVV 136

Query: 145 -------DEDQRSSSSNSSSSEY-------------NTLVDQNKRLKEENGALISELASM 204
                  D +     S+S SS Y               L+++N++L+ +N  L  EL  M
Sbjct: 137 SPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENEKLRSQNIQLNRELTQM 196

Query: 205 KNKCRELLDLVAKC------------GKKSSK---------------EEEVDERPKLFGV 233
           K+ C  +  L++              G  SS+               EEE +  P+LFGV
Sbjct: 197 KSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKRFSEMEIEEEEEASPRLFGV 256

BLAST of MC01g0189 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 141.7 bits (356), Expect = 8.1e-34
Identity = 84/200 (42.00%), Postives = 107/200 (53.50%), Query Frame = 0

Query: 26  KSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFVR 85
           K+ P PFL KTY+ V+DPATD V+SW  D T FVVW+  EFA+D+LP  FKH NFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 86  QLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAWTSNKQ---------------- 145
           QLNTYGFRK+   RWEF N+ F++GEK  LCEI RRK      Q                
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 146 ------------RVAEASEHHELDEDQRSSSSN------SSSSEYNTLVDQNKRLKEENG 192
                       RV    E H   +D   S          ++++   L + N+RL+  N 
Sbjct: 149 FSGGSFFPLPPPRVTTPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNT 208

BLAST of MC01g0189 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 131.3 bits (329), Expect = 1.1e-30
Identity = 84/210 (40.00%), Postives = 113/210 (53.81%), Query Frame = 0

Query: 25  RKSTPPPFLVKTYKAVEDPATDDVISWNGDGTAFVVWQTAEFAKDVLPTLFKHCNFSSFV 84
           ++S P PFL KTY+ VEDP  D++ISWN DGT F+VW+ AEFA+D+LP  FKH NFSSFV
Sbjct: 53  QRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSSFV 112

Query: 85  RQLNTYGFRKVATTRWEFCNDKFQKGEKEKLCEIRRRKAWTSNKQRVAEA---------- 144
           RQLNTYGFRKV   RWEF ND F++GEK  L +I+RRK         A A          
Sbjct: 113 RQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAASAV 172

Query: 145 ------------SEHHELDEDQRSSSSN--------------------SSSSEYNTLVDQ 193
                       S  +  +E   SS+S+                    +S +    LV++
Sbjct: 173 TVAAVPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAPELVEE 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222301.0e-4954.72Heat stress transcription factor B-3 OS=Arabidopsis thaliana OX=3702 GN=HSFB3 PE... [more]
P223352.1e-3949.73Heat shock factor protein HSF24 OS=Solanum peruvianum OX=4082 GN=HSF24 PE=2 SV=1[more]
Q6Z9C84.5e-3743.98Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q963202.2e-3644.53Heat stress transcription factor B-1 OS=Arabidopsis thaliana OX=3702 GN=HSFB1 PE... [more]
Q652B01.1e-3541.30Heat stress transcription factor B-2c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
XP_022152241.11.53e-17599.61heat stress transcription factor B-3-like [Momordica charantia][more]
XP_022967894.13.63e-11476.19heat stress transcription factor B-3 [Cucurbita maxima][more]
XP_038883399.19.35e-11475.89heat stress transcription factor B-3-like [Benincasa hispida][more]
XP_022928625.11.47e-11375.98heat stress transcription factor B-3 [Cucurbita moschata][more]
KAG7033512.12.97e-11375.98Heat stress transcription factor B-3, partial [Cucurbita argyrosperma subsp. arg... [more]
Match NameE-valueIdentityDescription
A0A6J1DH467.41e-17699.61heat stress transcription factor B-3-like OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1HY141.76e-11476.19heat stress transcription factor B-3 OS=Cucurbita maxima OX=3661 GN=LOC111467268... [more]
A0A6J1ES597.13e-11475.98heat stress transcription factor B-3 OS=Cucurbita moschata OX=3662 GN=LOC1114354... [more]
A0A5D3CGR49.83e-10571.09Heat stress transcription factor B-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A314YHN62.15e-10270.64Heat stress transcription factor B-3 OS=Prunus yedoensis var. nudiflora OX=20945... [more]
Match NameE-valueIdentityDescription
AT2G41690.17.3e-5154.72heat shock transcription factor B3 [more]
AT4G36990.11.6e-3744.53heat shock factor 4 [more]
AT5G62020.11.6e-3439.00heat shock transcription factor B2A [more]
AT1G46264.18.1e-3442.00heat shock transcription factor B4 [more]
AT4G11660.11.1e-3040.00winged-helix DNA-binding transcription factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 156..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..158
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..145
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..22
NoneNo IPR availablePANTHERPTHR10015:SF285HEAT STRESS TRANSCRIPTION FACTOR B-3coord: 22..247
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 70..82
score: 64.84
coord: 83..95
score: 87.03
coord: 32..55
score: 46.9
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 28..121
e-value: 1.2E-50
score: 184.3
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 32..121
e-value: 1.8E-31
score: 108.6
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 71..95
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 24..122
e-value: 3.3E-36
score: 125.9
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 22..247
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 28..121

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0189.1MC01g0189.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0043565 sequence-specific DNA binding