MC01g0153 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC01g0153
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionE3 ubiquitin-protein ligase RNF181-like
LocationMC01: 7258711 .. 7259277 (-)
RNA-Seq ExpressionMC01g0153
SyntenyMC01g0153
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATCAGCCGCATCAGGTCCTGGAAGTCCGATTGAAGCGTCTGAGCCGTCTGATATCGCGATCTCCAGCGGCGGGGGTTCCGCACCAGGTCCTCCAGGAAACTCCGCCGTCTCCGGTGGCGGACGCCGCGTTCGTTCTCCCTCTGCGCGATCCGCTGTTTCTCGAGGCCTACGTCCGGCAGTTGCTGTGCTCGCTGAATCTCCCCGCCGGCGCCTGCGGAGCCATCGGCCGGAGGATCGCGTCGTTCGTCGTCGAATTGGCGGGCGGAAACAGTGCGGCGAGCCTCCACATTGTGGCGTTGGTGAATTACATTGAGGAGTTTTGGATGGATCCGATCCCGGCGCCGGTGCCACCGAGCAGAGGGGCGGCGGAATCGGCGATCTCGAGGCTGAAGAAGGAGAAGGTTGAAGAAGGAGTAGAATTGGGAGATTGCAGCGTTTGTTTGAATGAGATGAATGGCGGGAGGCAGGTGATTAGGATTCCGTGCGGTCATGTGTATCATGAATCCTGCATCCTCACATGGCTGAGGAAGAGCAATTCTTGCCCATTGTGTAGAACAAAGTTGGAA

mRNA sequence

GATCAGCCGCATCAGGTCCTGGAAGTCCGATTGAAGCGTCTGAGCCGTCTGATATCGCGATCTCCAGCGGCGGGGGTTCCGCACCAGGTCCTCCAGGAAACTCCGCCGTCTCCGGTGGCGGACGCCGCGTTCGTTCTCCCTCTGCGCGATCCGCTGTTTCTCGAGGCCTACGTCCGGCAGTTGCTGTGCTCGCTGAATCTCCCCGCCGGCGCCTGCGGAGCCATCGGCCGGAGGATCGCGTCGTTCGTCGTCGAATTGGCGGGCGGAAACAGTGCGGCGAGCCTCCACATTGTGGCGTTGGTGAATTACATTGAGGAGTTTTGGATGGATCCGATCCCGGCGCCGGTGCCACCGAGCAGAGGGGCGGCGGAATCGGCGATCTCGAGGCTGAAGAAGGAGAAGGTTGAAGAAGGAGTAGAATTGGGAGATTGCAGCGTTTGTTTGAATGAGATGAATGGCGGGAGGCAGGTGATTAGGATTCCGTGCGGTCATGTGTATCATGAATCCTGCATCCTCACATGGCTGAGGAAGAGCAATTCTTGCCCATTGTGTAGAACAAAGTTGGAA

Coding sequence (CDS)

GATCAGCCGCATCAGGTCCTGGAAGTCCGATTGAAGCGTCTGAGCCGTCTGATATCGCGATCTCCAGCGGCGGGGGTTCCGCACCAGGTCCTCCAGGAAACTCCGCCGTCTCCGGTGGCGGACGCCGCGTTCGTTCTCCCTCTGCGCGATCCGCTGTTTCTCGAGGCCTACGTCCGGCAGTTGCTGTGCTCGCTGAATCTCCCCGCCGGCGCCTGCGGAGCCATCGGCCGGAGGATCGCGTCGTTCGTCGTCGAATTGGCGGGCGGAAACAGTGCGGCGAGCCTCCACATTGTGGCGTTGGTGAATTACATTGAGGAGTTTTGGATGGATCCGATCCCGGCGCCGGTGCCACCGAGCAGAGGGGCGGCGGAATCGGCGATCTCGAGGCTGAAGAAGGAGAAGGTTGAAGAAGGAGTAGAATTGGGAGATTGCAGCGTTTGTTTGAATGAGATGAATGGCGGGAGGCAGGTGATTAGGATTCCGTGCGGTCATGTGTATCATGAATCCTGCATCCTCACATGGCTGAGGAAGAGCAATTCTTGCCCATTGTGTAGAACAAAGTTGGAA

Protein sequence

DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRDPLFLEAYVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE
Homology
BLAST of MC01g0153 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.1e-12
Identity = 31/79 (39.24%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 110 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 169
           DP     PP+  +A  A+  +K  K     E+  C+VC++E   G  V ++PC HV+H+ 
Sbjct: 182 DPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQD 241

Query: 170 CILTWLRKSNSCPLCRTKL 189
           C+L WL   NSCP+CR +L
Sbjct: 242 CLLPWLELHNSCPVCRFEL 260

BLAST of MC01g0153 vs. ExPASy Swiss-Prot
Match: Q9VE61 (E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN=CG7694 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-11
Identity = 37/95 (38.95%), Postives = 54/95 (56.84%), Query Frame = 0

Query: 95  LHIVALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGG 154
           L ++A++N I+      I  P    R   E  +  + K   +EG +L +CSVC      G
Sbjct: 28  LQVLAIMNGIDM----EIEVPEASKRAILELPVHEIVKS--DEGGDL-ECSVCKEPAEEG 87

Query: 155 RQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 190
           ++   +PC H +HE CIL WL+K+NSCPLCR +LE
Sbjct: 88  QKYRILPCKHEFHEECILLWLKKTNSCPLCRYELE 115

BLAST of MC01g0153 vs. ExPASy Swiss-Prot
Match: Q5M974 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.8e-11
Identity = 35/83 (42.17%), Postives = 45/83 (54.22%), Query Frame = 0

Query: 108 WMDPIPAPVPPSRGAAESAISRLKKEKV--EEGVELGDCSVCLNEMNGGRQVIRIPCGHV 167
           W   +P P      AA+  +  L K  V  E+      C VCL E   G  V ++PC H+
Sbjct: 47  WDQRLPPP------AAKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQLPCEHL 106

Query: 168 YHESCILTWLRKSNSCPLCRTKL 189
           +H SCIL WL K+NSCPLCR +L
Sbjct: 107 FHSSCILPWLGKTNSCPLCRHEL 123

BLAST of MC01g0153 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 8.9e-11
Identity = 34/79 (43.04%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 110 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 169
           DP     PP   A + A+  L   K+EE V    CSVCL+++  G Q  ++PC H +H S
Sbjct: 171 DPSRNGTPP---AKKEAVEALPTVKIEEVV---SCSVCLDDLEVGSQAKQMPCEHKFHSS 230

Query: 170 CILTWLRKSNSCPLCRTKL 189
           CIL WL   +SCP+CR +L
Sbjct: 231 CILPWLELHSSCPVCRFEL 243

BLAST of MC01g0153 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-10
Identity = 28/79 (35.44%), Postives = 44/79 (55.70%), Query Frame = 0

Query: 110 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 169
           DP     PP+  +A  A+  +   K     E   C+VC+++   G +  ++PC H+YH+ 
Sbjct: 190 DPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKD 249

Query: 170 CILTWLRKSNSCPLCRTKL 189
           C+L WL   NSCP+CR +L
Sbjct: 250 CLLPWLELHNSCPVCRHEL 268

BLAST of MC01g0153 vs. NCBI nr
Match: XP_022151640.1 (E3 ubiquitin-protein ligase RNF181-like [Momordica charantia])

HSP 1 Score: 252 bits (643), Expect = 4.91e-82
Identity = 135/193 (69.95%), Postives = 155/193 (80.31%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPL---RDPLFLEAY 60
           DQPHQVL+VRL+  SR+ISRSPAA     +LQET  +P+A+A F LPL      LFLEAY
Sbjct: 24  DQPHQVLQVRLRSQSRMISRSPAA-----ILQETSWTPMANAVFPLPLSALNGLLFLEAY 83

Query: 61  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNS-AASLHIVALVNYIEEFWMDPIPAPV 120
           +RQLLCSL+LP GAC  I R+IASFVVELAGGNS   +LH+VALV+YIEE  +    AP 
Sbjct: 84  IRQLLCSLDLPEGACEVIARKIASFVVELAGGNSDQVNLHVVALVDYIEEMAIVAEEAPA 143

Query: 121 PPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHESCILTWLR 180
               GAA+SAISRLKKEKVEE VELGDCSVCLNEMNGG +VI++PCGHVYHESCIL+WL 
Sbjct: 144 ----GAAKSAISRLKKEKVEEEVELGDCSVCLNEMNGGSEVIKMPCGHVYHESCILSWLN 203

Query: 181 KSNSCPLCRTKLE 189
           K+NSCPLCRTKLE
Sbjct: 204 KNNSCPLCRTKLE 207

BLAST of MC01g0153 vs. NCBI nr
Match: XP_023539480.1 (E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 196 bits (498), Expect = 9.72e-60
Identity = 105/211 (49.76%), Postives = 139/211 (65.88%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 60
           D P Q+L++R+   SRL++ SP +G P  ++ ET P+ +ADA F LPLR   DPLF +AY
Sbjct: 17  DGPDQLLQIRIGHSSRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAY 76

Query: 61  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 120
           +++ L S NL A    AI RRIASFVV++A GN   + +IVA ++YI+ FWMD       
Sbjct: 77  IKEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWMDDSATAAE 136

Query: 121 PS----------------RGAAESAISRLKKEKVEE--GVELGDCSVCLNEM-NGGRQVI 180
                             RGA E+AI RLKKEK +   G +LG+CSVC +EM +GG +V 
Sbjct: 137 EEEDEEEETEIFDDFSGRRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGGEEVS 196

Query: 181 RIPCGHVYHESCILTWLRKSNSCPLCRTKLE 189
           RIPCGHVYH+SC+LTWL+++NSCPLCR KLE
Sbjct: 197 RIPCGHVYHKSCVLTWLQRNNSCPLCRNKLE 227

BLAST of MC01g0153 vs. NCBI nr
Match: XP_023539471.1 (uncharacterized protein LOC111800118 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 190 bits (482), Expect = 3.71e-57
Identity = 103/223 (46.19%), Postives = 139/223 (62.33%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 60
           D P Q+L++R++  +RL++ SP +G P  ++ ET P+ +ADA F LPLR   DPLF +AY
Sbjct: 17  DDPDQLLQIRIRHSTRLLTGSPFSGYPFNIVHETLPTTMADAQFPLPLRELEDPLFCQAY 76

Query: 61  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 120
           + + L S NL A    AI RRIASFVV++A GN   + +IVA ++YI+ FW+D       
Sbjct: 77  IEEFLSSYNLGATIKNAIARRIASFVVQMACGNYEMTFYIVAEIDYIQMFWLDQSAIAAE 136

Query: 121 PS----------------------------RGAAESAISRLKKEKVEE--GVELGDCSVC 180
                                         RGA E+AI RLKKEK +   G +LG+CSVC
Sbjct: 137 EEEEEEEDEEEEEDEEEEEEEVIFDNFSGRRGAPETAIERLKKEKFDGFGGEDLGNCSVC 196

Query: 181 LNEM-NGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 189
            +EM +GG +V RIPCGHVYH+SC+LTWL+++NSCPLCR KLE
Sbjct: 197 YDEMKDGGEEVSRIPCGHVYHKSCVLTWLQRNNSCPLCRKKLE 239

BLAST of MC01g0153 vs. NCBI nr
Match: KAG6596836.1 (hypothetical protein SDJN03_10016, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 177 bits (448), Expect = 4.98e-52
Identity = 99/222 (44.59%), Postives = 130/222 (58.56%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 60
           D P Q+L++R+    RL   SP  G P  ++ ET  +   DA F LPLR   DPLF  AY
Sbjct: 17  DGPDQLLQIRIGHSRRLFIGSPLPGYPFNIVYETLTTITVDAQFSLPLRELEDPLFCRAY 76

Query: 61  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 120
           + + L S NL A    AI +RIASFVV++A GN   + +IVA ++YI+ FW+D       
Sbjct: 77  IEEFLSSYNLDATINNAIAQRIASFVVQMACGNYEMTFYIVAEIDYIQMFWLDQSAIAAE 136

Query: 121 PS---------------------------RGAAESAISRLKKEKVEE--GVELGDCSVCL 180
                                        RGA E+AI RLKKEK +   G +LG+CSVC 
Sbjct: 137 EEEEEEEEEDEEEEEEEEEEVIFDNFSERRGAPETAIERLKKEKFDGFGGEDLGNCSVCY 196

Query: 181 NEMN-GGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 189
           +EM  GG ++IRIPCGHVYH+SC+LTWL+++NSCPLCR K+E
Sbjct: 197 DEMKEGGEELIRIPCGHVYHKSCVLTWLQRNNSCPLCRKKIE 238

BLAST of MC01g0153 vs. NCBI nr
Match: XP_023539485.1 (RING-H2 finger protein ATL80-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 167 bits (424), Expect = 1.71e-48
Identity = 96/215 (44.65%), Postives = 130/215 (60.47%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAY 60
           D  +Q+L++R+   +RL+  S   G P  ++ ET  + +ADA F LPLR   DPLF  AY
Sbjct: 17  DGLYQLLQIRIGHSTRLLIGSSFPGYPFNIVYETLTTRMADAQFPLPLRELEDPLFCRAY 76

Query: 61  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVP 120
           + + L S NL A    AI +RI SFVV++A GN   + +IVA ++YI+ FW+D       
Sbjct: 77  IEEFLSSYNLDATINNAIAQRITSFVVQMACGNYEMTFYIVAEIDYIQMFWLDQSAIATE 136

Query: 121 PS--------------------RGAAESAISRLKKEKVEE--GVELGDCSVCLNEM-NGG 180
                                 RGA E+AI RLKKEK +   G +LG+CSVC +EM +GG
Sbjct: 137 EEEEEEDEEEEVEVIFDNFSERRGAPETAIERLKKEKFDGFGGEDLGNCSVCYDEMKDGG 196

Query: 181 RQVIRIPCGHVYHESCILTWLRKSNSCPLCRTKLE 189
            +V RI CGHVYH+SC+LTWL+++NSCPL R KLE
Sbjct: 197 EEVSRILCGHVYHKSCVLTWLQRNNSCPLWRKKLE 231

BLAST of MC01g0153 vs. ExPASy TrEMBL
Match: A0A6J1DBR5 (E3 ubiquitin-protein ligase RNF181-like OS=Momordica charantia OX=3673 GN=LOC111019547 PE=4 SV=1)

HSP 1 Score: 252 bits (643), Expect = 2.38e-82
Identity = 135/193 (69.95%), Postives = 155/193 (80.31%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPL---RDPLFLEAY 60
           DQPHQVL+VRL+  SR+ISRSPAA     +LQET  +P+A+A F LPL      LFLEAY
Sbjct: 24  DQPHQVLQVRLRSQSRMISRSPAA-----ILQETSWTPMANAVFPLPLSALNGLLFLEAY 83

Query: 61  VRQLLCSLNLPAGACGAIGRRIASFVVELAGGNS-AASLHIVALVNYIEEFWMDPIPAPV 120
           +RQLLCSL+LP GAC  I R+IASFVVELAGGNS   +LH+VALV+YIEE  +    AP 
Sbjct: 84  IRQLLCSLDLPEGACEVIARKIASFVVELAGGNSDQVNLHVVALVDYIEEMAIVAEEAPA 143

Query: 121 PPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHESCILTWLR 180
               GAA+SAISRLKKEKVEE VELGDCSVCLNEMNGG +VI++PCGHVYHESCIL+WL 
Sbjct: 144 ----GAAKSAISRLKKEKVEEEVELGDCSVCLNEMNGGSEVIKMPCGHVYHESCILSWLN 203

Query: 181 KSNSCPLCRTKLE 189
           K+NSCPLCRTKLE
Sbjct: 204 KNNSCPLCRTKLE 207

BLAST of MC01g0153 vs. ExPASy TrEMBL
Match: A0A6J1L186 (NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4 SV=1)

HSP 1 Score: 166 bits (420), Expect = 1.89e-48
Identity = 92/195 (47.18%), Postives = 125/195 (64.10%), Query Frame = 0

Query: 5   QVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLR---DPLFLEAYVRQL 64
           Q L  RL   SRLISRSP+AG+P+ +L+ETP +P+ADA F LPLR   DPLF  AY+ +L
Sbjct: 21  QFLRFRLCYSSRLISRSPSAGLPYDILEETPSTPMADALFPLPLRELEDPLFCHAYLIEL 80

Query: 65  LCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPVPPSRG 124
           + SLNL + +C  I ++I S V + A  +  A   +VA ++YI+ FWM+  P  V   RG
Sbjct: 81  VSSLNLDSMSCNPIVQKITSLVTQWAAVDYDAKFQLVADIDYIQMFWMEKRPGSV--GRG 140

Query: 125 AAESAISRLKKEKV----EEGVELGDCSVCLN----EMNGGRQVIRIPCGHVYHESCILT 184
                   LK+ +V    ++G+   +CSVC      E  G R+V +IPCGHV+H+SCILT
Sbjct: 141 VMVEEKVVLKEAEVAAAEKQGIGGEECSVCYESYEAEKGGEREVAKIPCGHVFHKSCILT 200

Query: 185 WLRKSNSCPLCRTKL 188
           W ++SNSCPLCR KL
Sbjct: 201 WFQRSNSCPLCRAKL 213

BLAST of MC01g0153 vs. ExPASy TrEMBL
Match: A0A6J1G7Z5 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC111451666 PE=4 SV=1)

HSP 1 Score: 164 bits (416), Expect = 1.02e-47
Identity = 94/201 (46.77%), Postives = 118/201 (58.71%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRD----PLFLEA 60
            QP Q+LE+RL   + LISRS       Q+LQ+TP +P+ DA F   LRD    P     
Sbjct: 27  QQPMQLLELRLGNSTHLISRSS-----RQILQQTPTTPMGDALFPFVLRDLQHPPFLSNC 86

Query: 61  YVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPV 120
           YV Q L S ++P  AC  I   I+SF ++LA GN   + HIV  V+ IE  W+D  P  V
Sbjct: 87  YVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNVDLNFHIVVEVDVIEMIWVDLDPFLV 146

Query: 121 PP-----SRGAAESAISRLKKEKVE---EGVELGDCSVCLNEMNGGRQVIRIPCGHVYHE 180
                    GA  SAI RL++++ +   E  E GDCSVC   + G  +V RIPCGHVYH+
Sbjct: 147 EEYSPAEMNGAPASAIERLQRQEFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHK 206

Query: 181 SCILTWLRKSNSCPLCRTKLE 189
            CIL WL+ SNSCPLCRTKLE
Sbjct: 207 CCILKWLQMSNSCPLCRTKLE 222

BLAST of MC01g0153 vs. ExPASy TrEMBL
Match: A0A6J1KYV1 (E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499496 PE=4 SV=1)

HSP 1 Score: 164 bits (414), Expect = 2.05e-47
Identity = 94/201 (46.77%), Postives = 117/201 (58.21%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRD----PLFLEA 60
            QP Q+LE+RL   + LISRS       Q+LQ+TP +P+ DA F   LRD    P     
Sbjct: 27  QQPIQLLELRLGHSTHLISRSS-----RQILQQTPSTPIGDALFPFVLRDLQHPPFLSNC 86

Query: 61  YVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPV 120
           YV Q L S ++P  AC  I   I+SF ++LA GN   + HIV  V+  E  W+D  P  V
Sbjct: 87  YVLQFLSSFSIPIAACDIIYEEISSFALQLAAGNMDLNFHIVVEVDVNEMIWVDLDPFLV 146

Query: 121 PP-----SRGAAESAISRLKKEKVE---EGVELGDCSVCLNEMNGGRQVIRIPCGHVYHE 180
                    GA  SAI RL+++K +   E  E GDCSVC   + G  +V RIPCGHVYH+
Sbjct: 147 EEYSPAEMNGAPASAIERLQRQKFDGLREEEEEGDCSVCCEALKGEEEVSRIPCGHVYHK 206

Query: 181 SCILTWLRKSNSCPLCRTKLE 189
            CIL WL+ SNSCPLCRTKLE
Sbjct: 207 FCILKWLQMSNSCPLCRTKLE 222

BLAST of MC01g0153 vs. ExPASy TrEMBL
Match: A0A6J1G809 (probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC111451600 PE=4 SV=1)

HSP 1 Score: 157 bits (397), Expect = 7.32e-45
Identity = 89/200 (44.50%), Postives = 118/200 (59.00%), Query Frame = 0

Query: 1   DQPHQVLEVRLKRLSRLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRD----PLFLEA 60
           +QP Q +E+RL   S LISRS       Q+LQ+TP +P+ DA F   LRD    P   ++
Sbjct: 27  EQPMQFVELRLGHSSHLISRSS-----RQILQQTPSTPMGDALFPFILRDLLHPPFLSKS 86

Query: 61  YVRQLLCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPV 120
           +V + L S ++P   C  I   I+SF + LA GN   + HIV  V+ IE  W+D  P  +
Sbjct: 87  HVLRFLSSFSIPIAVCNIIFVEISSFALHLAAGNVDLNFHIVVDVDAIEMIWVDLDPFLI 146

Query: 121 -----PPSRGAAESAISRLKKEKVE--EGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 180
                P   GA  SAI RL++++ +     E G+CSVC   + G  +V RIPCGHVYH+ 
Sbjct: 147 EEYSPPAMNGAPASAIERLQRQEFDGLREEEEGECSVCCEALKGEEEVSRIPCGHVYHKC 206

Query: 181 CILTWLRKSNSCPLCRTKLE 189
           CIL WL+ SNSCPLCRTKLE
Sbjct: 207 CILRWLQMSNSCPLCRTKLE 221

BLAST of MC01g0153 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.7e-13
Identity = 31/79 (39.24%), Postives = 46/79 (58.23%), Query Frame = 0

Query: 110 DPIPAPVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHES 169
           DP     PP+  +A  A+  +K  K     E+  C+VC++E   G  V ++PC HV+H+ 
Sbjct: 182 DPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQD 241

Query: 170 CILTWLRKSNSCPLCRTKL 189
           C+L WL   NSCP+CR +L
Sbjct: 242 CLLPWLELHNSCPVCRFEL 260

BLAST of MC01g0153 vs. TAIR 10
Match: AT5G54990.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.7e-13
Identity = 24/45 (53.33%), Postives = 35/45 (77.78%), Query Frame = 0

Query: 143 DCSVCLNEMNGGRQVIRIPCGHVYHESCILTWLRKSNSCPLCRTK 188
           DC +CL E++ G   +++PC HV+H  CI+TWL+K+ SCP+CRTK
Sbjct: 173 DCPICLTELSSGVSRMKLPCSHVFHRDCIMTWLKKNPSCPICRTK 217

BLAST of MC01g0153 vs. TAIR 10
Match: AT1G60360.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 9.8e-13
Identity = 34/93 (36.56%), Postives = 49/93 (52.69%), Query Frame = 0

Query: 99  ALVNYIEEFWMDPIPAPVPPSRGAAESAISRLKKEKVEE---GVELGDCSVCLNEMNGGR 158
           +L   IE+   D  P P P    A+E  I+ L   K+       ++  C+VC+ E   G 
Sbjct: 180 SLEQLIEQLTQDDRPGPPP----ASEPTINSLPSVKITPQHLTNDMSQCTVCMEEFIVGG 239

Query: 159 QVIRIPCGHVYHESCILTWLRKSNSCPLCRTKL 189
               +PC H+YH+ CI+ WLR +NSCP+CR  L
Sbjct: 240 DATELPCKHIYHKDCIVPWLRLNNSCPICRRDL 268

BLAST of MC01g0153 vs. TAIR 10
Match: AT4G05350.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 3.7e-12
Identity = 50/196 (25.51%), Postives = 92/196 (46.94%), Query Frame = 0

Query: 2   QPHQVLEVRLKRLS-RLISRSPAAGVPHQVLQETPPSPVADAAFVLPLRDPLFLEAYVRQ 61
           +P+ V+ +RLK  S  +I  +   G  ++  Q +PP       F++ +        ++R 
Sbjct: 23  EPNSVI-IRLKTKSVEIIIENQTTGRQNRRTQVSPP-------FLIDINLRSSSHDHIRT 82

Query: 62  L----LCSLNLPAGACGAIGRRIASFVVELAGGNSAASLHIVALVNYIEEFWMDPIPAPV 121
           L    L      +  C  +  +I++  ++L  G++  +L + A V Y        +    
Sbjct: 83  LLHDRLVGYPFESYLCDDLAPKISTAAIDLGFGHNGFTLTMAATVTY------RTVSVMS 142

Query: 122 PPSRGAAESAISRLKKEKVEE-GVELGDCSVCLNEMNGG---RQVIRIPCGHVYHESCIL 181
              +      + R+K E+ +   +E   CS+CL  +  G   R V R+ C HV+H  C+L
Sbjct: 143 NDEKSLRMVLLGRMKAEEFKSLKMETEPCSICLESLVSGPKPRDVTRMTCSHVFHNGCLL 202

Query: 182 TWLRKSNSCPLCRTKL 189
            WL++ N+CPLCRT++
Sbjct: 203 EWLKRKNTCPLCRTEI 204

BLAST of MC01g0153 vs. TAIR 10
Match: AT1G18770.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-11
Identity = 28/74 (37.84%), Postives = 43/74 (58.11%), Query Frame = 0

Query: 115 PVPPSRGAAESAISRLKKEKVEEGVELGDCSVCLNEMNGGRQVIRIPCGHVYHESCILTW 174
           P P S+   +S   ++ K       E+  C +CL E + GR+V+ +PCGH + + C+L W
Sbjct: 32  PTPASKLVVKSLARKIYKMTTSSTGEM--CIICLEEFSEGRRVVTLPCGHDFDDECVLKW 91

Query: 175 LRKSNSCPLCRTKL 189
              ++SCPLCR KL
Sbjct: 92  FETNHSCPLCRFKL 103

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPN78.1e-1239.24E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Q9VE612.3e-1138.95E3 ubiquitin-protein ligase RNF181 homolog OS=Drosophila melanogaster OX=7227 GN... [more]
Q5M9746.8e-1142.17E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 ... [more]
Q6AVN28.9e-1143.04E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI... [more]
P0CH301.2e-1035.44E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_022151640.14.91e-8269.95E3 ubiquitin-protein ligase RNF181-like [Momordica charantia][more]
XP_023539480.19.72e-6049.76E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo][more]
XP_023539471.13.71e-5746.19uncharacterized protein LOC111800118 [Cucurbita pepo subsp. pepo][more]
KAG6596836.14.98e-5244.59hypothetical protein SDJN03_10016, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023539485.11.71e-4844.65RING-H2 finger protein ATL80-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1DBR52.38e-8269.95E3 ubiquitin-protein ligase RNF181-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A6J1L1861.89e-4847.18NEP1-interacting protein-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111499495 PE=4... [more]
A0A6J1G7Z51.02e-4746.77E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KYV12.05e-4746.77E3 ubiquitin-protein ligase RNF126-like OS=Cucurbita maxima OX=3661 GN=LOC111499... [more]
A0A6J1G8097.32e-4544.50probable E3 ubiquitin-protein ligase RHA4A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT3G19950.15.7e-1339.24RING/U-box superfamily protein [more]
AT5G54990.15.7e-1353.33RING/U-box superfamily protein [more]
AT1G60360.19.8e-1336.56RING/U-box superfamily protein [more]
AT4G05350.13.7e-1225.51RING/U-box superfamily protein [more]
AT1G18770.11.1e-1137.84RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 144..184
e-value: 1.0E-8
score: 44.9
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 143..185
e-value: 2.1E-15
score: 56.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 144..185
score: 13.374458
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 116..189
e-value: 6.2E-21
score: 75.9
NoneNo IPR availablePANTHERPTHR45931SI:CH211-59O9.10coord: 58..189
NoneNo IPR availablePANTHERPTHR45931:SF9E3 UBIQUITIN-PROTEIN LIGASE RNF12-B-LIKEcoord: 58..189
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 143..188

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC01g0153.1MC01g0153.1mRNA