MC01g0111 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0111
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	zf-RVT domain-containing protein
Location	MC01: 5921917 .. 5922349 (-)
RNA-Seq Expression	MC01g0111
Synteny	MC01g0111

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAGGTTTCGGTGTTTTCTTGGATAGTCCTTTTTCGGAACCTAAATACCTCTAATGTTCTTCAGAAAAATCTTCCGTTTTTAGCTCTCCATCCATCCATTTGTGTGCTTTGTATGGCACGAGAAGACACTCAAAATCATGCACTGTTTTGGTGTCCTTTTGCAGCAAGCTGCTGGAAGCTTTTGCTTGCTTCCTTCAATCTCCAGTGGGTTTTTTCTGATTCGGCCAGAGGCAATTTAAATCAGCTTCTTAATGGTCCCAACGTCAAGCCTTTAGCTCGGCTTCTATGGTAATTCAGTGAAAGCTTTAATTTATGAATTGTGGTTTGAAAGAAACCAAAGGATCTTCGAAGATAAGAAGAGAGATTGGTTCGACATTCTAGATTCGGCAAAATTTAAAGCTTCCCAGTGGTGTTTGCTCTCGCTATTGTTC

mRNA sequence

AAGAAGGTTTCGGTGTTTTCTTGGATAGTCCTTTTTCGGAACCTAAATACCTCTAATGTTCTTCAGAAAAATCTTCCGTTTTTAGCTCTCCATCCATCCATTTGTGTGCTTTGTATGGCACGAGAAGACACTCAAAATCATGCACTGTTTTGGTGTCCTTTTGCAGCAAGCTGCTGGAAGCTTTTGCTTGCTTCCTTCAATCTCCAGTGGGTTTTTTCTGATTCGGCCAGAGGCAATTTAAATCAGCTTCTTAATGGTCCCAACGTCAAGCCTTTAGCTCGGCTTCTATGGAATTCAGTGAAAGCTTTAATTTATGAATTGTGGTTTGAAAGAAACCAAAGGATCTTCGAAGATAAGAAGAGAGATTGGTTCGACATTCTAGATTCGGCAAAATTTAAAGCTTCCCAGTGGTGTTTGCTCTCGCTATTGTTC

Coding sequence (CDS)

AAGAAGGTTTCGGTGTTTTCTTGGATAGTCCTTTTTCGGAACCTAAATACCTCTAATGTTCTTCAGAAAAATCTTCCGTTTTTAGCTCTCCATCCATCCATTTGTGTGCTTTGTATGGCACGAGAAGACACTCAAAATCATGCACTGTTTTGGTGTCCTTTTGCAGCAAGCTGCTGGAAGCTTTTGCTTGCTTCCTTCAATCTCCAGTGGGTTTTTTCTGATTCGGCCAGAGGCAATTTAAATCAGCTTCTTAATGGTCCCAACGTCAAGCCTTTAGCTCGGCTTCTATGGAATTCAGTGAAAGCTTTAATTTATGAATTGTGGTTTGAAAGAAACCAAAGGATCTTCGAAGATAAGAAGAGAGATTGGTTCGACATTCTAGATTCGGCAAAATTTAAAGCTTCCCAGTGGTGTTTGCTCTCGCTATTGTTC

Protein sequence

KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWKLLLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLWNSVKALIYELWFERNQRIFEDKKRDWFDILDSAKFKASQWCLLSLLF

Homology

[Close]

BLAST of MC01g0111 vs. NCBI nr
Match: XP_022153214.1 (uncharacterized protein LOC111020765 [Momordica charantia])

HSP 1 Score: 135 bits (340), Expect = 6.89e-37
Identity = 68/142 (47.89%), Postives = 90/142 (63.38%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           ++V+VF WI+    LNT++++QK  P  AL PS C LC    +  +H  F C FA+ CW 
Sbjct: 39  RRVNVFCWILFQGKLNTADIIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYFASKCWN 98

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGP-NVKPLARLLW-NSVKALIYELWFERNQRIFED 120
           LL   FN+ W F   A  N+ QLL+GP ++    R LW N VKAL+ ELWFERN R+FE+
Sbjct: 99  LLFHQFNVDWCFDLKAGDNVYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERNSRLFEE 158

Query: 121 KKRDWFDILDSAKFKASQWCLL 140
           K+R + +   SAKFKAS WC L
Sbjct: 159 KRRLFDESFYSAKFKASLWCSL 180

[Close]

BLAST of MC01g0111 vs. NCBI nr
Match: XP_038903695.1 (uncharacterized protein LOC120090219 [Benincasa hispida])

HSP 1 Score: 127 bits (320), Expect = 1.24e-32
Identity = 62/142 (43.66%), Postives = 87/142 (61.27%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           ++V++  WI+LF  LN + VLQK  P  +L P++C  C+   +   H  F CP+++ CW 
Sbjct: 154 RRVNILIWIMLFGQLNVAEVLQKKQPTPSLSPTVCPFCLHHSEVSLHLFFTCPYSSWCWN 213

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLW-NSVKALIYELWFERNQRIFEDK 120
            LL  FNL     +  + N+ QLL  P      RLLW N+VKAL+ +LWFERNQRIF +K
Sbjct: 214 KLLCFFNLPLTLCNDFKSNVFQLLARPTSHKSTRLLWCNAVKALLADLWFERNQRIFYNK 273

Query: 121 KRDWFDILDSAKFKASQWCLLS 141
                D L++A+ +AS WCLLS
Sbjct: 274 ATSCQDRLEAARRQASSWCLLS 295

[Close]

BLAST of MC01g0111 vs. NCBI nr
Match: TYK21876.1 (hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa])

HSP 1 Score: 117 bits (292), Expect = 4.84e-28
Identity = 54/141 (38.30%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           +++++  WI++FR +N+S +LQK  P + + PSIC LC+       H   +CP ++  W+
Sbjct: 224 RRINILIWIMVFRFVNSSEILQKKSP-INVSPSICPLCLKASKNLPHIFLYCPVSSFGWE 283

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLWNSV-KALIYELWFERNQRIFEDK 120
            + + FNL W F  S   ++ QLL+G N+    R++W  + KAL+ E+W ERNQRIF DK
Sbjct: 284 RIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDK 343

Query: 121 KRDWFDILDSAKFKASQWCLL 140
            R   +I+ +A   A+ WC L
Sbjct: 344 ARQRAEIMHAADLNAAAWCSL 363

[Close]

BLAST of MC01g0111 vs. NCBI nr
Match: KAA0035739.1 (hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa])

HSP 1 Score: 113 bits (283), Expect = 1.87e-26
Identity = 53/141 (37.59%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           +++++  WI++FR + +S +LQK  P + + PSIC LC+       H   +CP ++  W+
Sbjct: 224 RRINILIWIMVFRFVISSEILQKKSP-INVSPSICPLCLKASKNLPHIFLYCPVSSFGWE 283

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLWNSV-KALIYELWFERNQRIFEDK 120
            + + FNL W F  S   ++ QLL+G N+    R++W  + KAL+ E+W ERNQRIF DK
Sbjct: 284 RIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDK 343

Query: 121 KRDWFDILDSAKFKASQWCLL 140
            R   +I+ +A   A+ WC L
Sbjct: 344 ARQRAEIMHAADLNAAAWCSL 363

[Close]

BLAST of MC01g0111 vs. NCBI nr
Match: KAA0062564.1 (GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 105 bits (262), Expect = 3.89e-23
Identity = 55/126 (43.65%), Postives = 75/126 (59.52%), Query Frame = 0


Query: 18  SNVLQKNLPFLALHPSICVLCMAR-EDTQNHALFWCPFAASCWKLLLASFNLQWVFSDSA 77
           S V+Q+ L    L PS C LC+   ED Q   LF CP++  CW+ LL+ F + W F  S 
Sbjct: 556 SLVMQRELLNSCLMPSACSLCLEEGEDLQP--LFTCPYSVKCWENLLSLFGVDWAFDGSF 615

Query: 78  RGNLNQLLNGPNVKPLARLLW-NSVKALIYELWFERNQRIFEDKKRDWFDILDSAKFKAS 137
             N+ Q+L G ++K   RL+W N  KAL+ ++WFE NQRIF  K   W + LD AK  A+
Sbjct: 616 NSNIKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMHWSERLDIAKRNAA 675

Query: 138 QWCLLS 141
            WC+L+
Sbjct: 676 TWCMLN 679

[Close]

BLAST of MC01g0111 vs. ExPASy TrEMBL
Match: A0A6J1DIE2 (uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020765 PE=4 SV=1)

HSP 1 Score: 135 bits (340), Expect = 3.34e-37
Identity = 68/142 (47.89%), Postives = 90/142 (63.38%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           ++V+VF WI+    LNT++++QK  P  AL PS C LC    +  +H  F C FA+ CW 
Sbjct: 39  RRVNVFCWILFQGKLNTADIIQKKSPSDALLPSFCCLCTKSGEDHDHLFFHCYFASKCWN 98

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGP-NVKPLARLLW-NSVKALIYELWFERNQRIFED 120
           LL   FN+ W F   A  N+ QLL+GP ++    R LW N VKAL+ ELWFERN R+FE+
Sbjct: 99  LLFHQFNVDWCFDLKAGDNVYQLLHGPPHLSSSVRFLWLNVVKALLSELWFERNSRLFEE 158

Query: 121 KKRDWFDILDSAKFKASQWCLL 140
           K+R + +   SAKFKAS WC L
Sbjct: 159 KRRLFDESFYSAKFKASLWCSL 180

[Close]

BLAST of MC01g0111 vs. ExPASy TrEMBL
Match: A0A5D3DE60 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold494G00090 PE=4 SV=1)

HSP 1 Score: 117 bits (292), Expect = 2.34e-28
Identity = 54/141 (38.30%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           +++++  WI++FR +N+S +LQK  P + + PSIC LC+       H   +CP ++  W+
Sbjct: 224 RRINILIWIMVFRFVNSSEILQKKSP-INVSPSICPLCLKASKNLPHIFLYCPVSSFGWE 283

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLWNSV-KALIYELWFERNQRIFEDK 120
            + + FNL W F  S   ++ QLL+G N+    R++W  + KAL+ E+W ERNQRIF DK
Sbjct: 284 RIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDK 343

Query: 121 KRDWFDILDSAKFKASQWCLL 140
            R   +I+ +A   A+ WC L
Sbjct: 344 ARQRAEIMHAADLNAAAWCSL 363

[Close]

BLAST of MC01g0111 vs. ExPASy TrEMBL
Match: A0A5A7T2Y0 (zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold403G00100 PE=4 SV=1)

HSP 1 Score: 113 bits (283), Expect = 9.04e-27
Identity = 53/141 (37.59%), Postives = 85/141 (60.28%), Query Frame = 0


Query: 1   KKVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWK 60
           +++++  WI++FR + +S +LQK  P + + PSIC LC+       H   +CP ++  W+
Sbjct: 224 RRINILIWIMVFRFVISSEILQKKSP-INVSPSICPLCLKASKNLPHIFLYCPVSSFGWE 283

Query: 61  LLLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLWNSV-KALIYELWFERNQRIFEDK 120
            + + FNL W F  S   ++ QLL+G N+    R++W  + KAL+ E+W ERNQRIF DK
Sbjct: 284 RIFSLFNLVWNFDSSLSASVIQLLSGSNLPKTPRIIWEILSKALLIEIWIERNQRIFHDK 343

Query: 121 KRDWFDILDSAKFKASQWCLL 140
            R   +I+ +A   A+ WC L
Sbjct: 344 ARQRAEIMHAADLNAAAWCSL 363

[Close]

BLAST of MC01g0111 vs. ExPASy TrEMBL
Match: A0A5A7V5N8 (GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G00520 PE=3 SV=1)

HSP 1 Score: 105 bits (262), Expect = 1.88e-23
Identity = 55/126 (43.65%), Postives = 75/126 (59.52%), Query Frame = 0


Query: 18  SNVLQKNLPFLALHPSICVLCMAR-EDTQNHALFWCPFAASCWKLLLASFNLQWVFSDSA 77
           S V+Q+ L    L PS C LC+   ED Q   LF CP++  CW+ LL+ F + W F  S 
Sbjct: 556 SLVMQRELLNSCLMPSACSLCLEEGEDLQP--LFTCPYSVKCWENLLSLFGVDWAFDGSF 615

Query: 78  RGNLNQLLNGPNVKPLARLLW-NSVKALIYELWFERNQRIFEDKKRDWFDILDSAKFKAS 137
             N+ Q+L G ++K   RL+W N  KAL+ ++WFE NQRIF  K   W + LD AK  A+
Sbjct: 616 NSNIKQILLGSSLKEGPRLIWGNMSKALLSDIWFECNQRIFRGKVMHWSERLDIAKRNAA 675

Query: 138 QWCLLS 141
            WC+L+
Sbjct: 676 TWCMLN 679

[Close]

BLAST of MC01g0111 vs. ExPASy TrEMBL
Match: A0A438GR07 (zf-RVT domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_052070 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.98e-21
Identity = 53/144 (36.81%), Postives = 73/144 (50.69%), Query Frame = 0


Query: 2   KVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWKL 61
           KV  F W+V  + +NT+++LQ   P+ AL P IC LCM   +T +H    C      W  
Sbjct: 175 KVKSFVWLVAHKKVNTNDLLQLRRPYKALSPDICKLCMKHGETVDHLFLHCSLTIGLWHR 234

Query: 62  LLASFNLQWVFSDSARGNLNQLLNGPNVKPLARLLW-NSVKALIYELWFERNQRIFEDKK 121
           L  S  + WV   S    L+   NG        +LW N+  AL++ +W+ERN RIFEDK 
Sbjct: 235 LFQSAKMDWVSPRSISDMLSSNFNGFGSSKRGIVLWQNACIALMWVVWWERNARIFEDKA 294

Query: 122 RDWFDILDSAKFKASQWCLLSLLF 144
           R+   + DS  F  S W   S +F
Sbjct: 295 RNSEYLWDSICFLTSFWAFCSKVF 318

[Close]

BLAST of MC01g0111 vs. TAIR 10
Match: AT1G45063.1 (copper ion binding;electron carriers )

HSP 1 Score: 54.3 bits (129), Expect = 9.4e-08
Identity = 30/90 (33.33%), Postives = 49/90 (54.44%), Query Frame = 0


Query: 32  PSICVLCMAREDTQNHALFWCPFAASCWKLLLASFNL--QWVFSDSARGNLNQLLNGPNV 91
           PS+C+LC A ++T+ H  F CPF+   W    ++  +    +F DSAR     L +    
Sbjct: 281 PSLCLLCNALDETRQHVFFDCPFSHEVWSFFCSNARVTPPRMFKDSAR----WLRHPCRD 340

Query: 92  KPLARLLWNSVKALIYELWFERNQRIFEDK 120
           K +A +L  + +A +Y +W ERN R+  +K
Sbjct: 341 KKVAFILKLAYQASVYHIWRERNIRLNSNK 366

[Close]

BLAST of MC01g0111 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 6.1e-07
Identity = 40/141 (28.37%), Postives = 62/141 (43.97%), Query Frame = 0


Query: 2   KVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWKL 61
           K+  F W  L + L T+  L      + + PS C  C    ++ NHALF CPFA   W+L
Sbjct: 170 KLKHFLWRALSQALATTERLTTR--GMRIDPS-CPRCHRENESINHALFTCPFATMAWRL 229

Query: 62  LLASFNLQWVFSDSARGNLNQLLN---GPNVKPLARLL--WNSVKALIYELWFERNQRIF 121
             +S     + S+    N++ +LN      +    +LL  W     LI+ +W  RN  +F
Sbjct: 230 SDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLLPVW-----LIWRIWKARNNVVF 289

Query: 122 EDKKRDWFDILDSAKFKASQW 138
              +      + SAK +   W
Sbjct: 290 NKFRESPSKTVLSAKAETHDW 302

[Close]

BLAST of MC01g0111 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 2.6e-05
Identity = 34/138 (24.64%), Postives = 57/138 (41.30%), Query Frame = 0


Query: 2   KVSVFSWIVLFRNLNTSNVLQKNLPFLALHPSICVLCMAREDTQNHALFWCPFAASCWKL 61
           K+  F W +L   L T + L++    +  HP  C  C   ++T  H  F C +A   W+ 
Sbjct: 26  KIKHFLWKLLSGALATGDNLKRR--HIRNHPQ-CHRCCQEDETSQHLFFDCFYAQQVWRA 85

Query: 62  LLASFNLQWVFSDSARGNLNQLLNG--PNVKPLARLLWNSVKALIYELWFERNQRIFEDK 121
                        +    +  LL+    N +P    L+N    +++ LW  RNQ +F+ K
Sbjct: 86  SGIPHQELRTTGITMETKMELLLSSCLANRQP---QLFNLAIWILWRLWKSRNQLVFQQK 145

Query: 122 KRDWFDILDSAKFKASQW 138
              W + L  A+    +W
Sbjct: 146 SISWQNTLQRARNDVQEW 157

The following BLAST results are available for this feature:

BLAST of MC01g0111 vs.


Match Name	E-value	Identity	Description

BLAST of MC01g0111 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153214.1	6.89e-37	47.89	uncharacterized protein LOC111020765 [Momordica charantia]	[more]
XP_038903695.1	1.24e-32	43.66	uncharacterized protein LOC120090219 [Benincasa hispida]	[more]
TYK21876.1	4.84e-28	38.30	hypothetical protein E5676_scaffold494G00090 [Cucumis melo var. makuwa]	[more]
KAA0035739.1	1.87e-26	37.59	hypothetical protein E6C27_scaffold403G00100 [Cucumis melo var. makuwa]	[more]
KAA0062564.1	3.89e-23	43.65	GPI-anchor transamidase isoform X1 [Cucumis melo var. makuwa]	[more]

BLAST of MC01g0111 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIE2	3.34e-37	47.89	uncharacterized protein LOC111020765 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A5D3DE60	2.34e-28	38.30	zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A5A7T2Y0	9.04e-27	37.59	zf-RVT domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A5A7V5N8	1.88e-23	43.65	GPI-anchor transamidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A438GR07	4.98e-21	36.81	zf-RVT domain-containing protein OS=Vitis vinifera OX=29760 GN=CK203_052070 PE=4...	[more]

BLAST of MC01g0111 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G45063.1	9.4e-08	33.33	copper ion binding;electron carriers	[more]
AT3G09510.1	6.1e-07	28.37	Ribonuclease H-like superfamily protein	[more]
AT3G25270.1	2.6e-05	24.64	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026960	Reverse transcriptase zinc-binding domain	PFAM	PF13966	zf-RVT	coord: 2..59 e-value: 6.9E-8 score: 33.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0111.1	MC01g0111.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044249	cellular biosynthetic process
biological_process	GO:0044267	cellular protein metabolic process
biological_process	GO:1901566	organonitrogen compound biosynthetic process
biological_process	GO:0090407	organophosphate biosynthetic process
biological_process	GO:0006796	phosphate-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0016787	hydrolase activity