MC01g0029 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0029
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Protein TIC 214
Location	MC01: 2086502 .. 2086714 (+)
RNA-Seq Expression	MC01g0029
Synteny	MC01g0029

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTTTTAAATCTTTTATACTAGGTAATCTAGTATCCTTCTGCATGAAGATAATCAATTCAGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTCTCCATAGGGCCCTCTTATCTCTTCCTTCTCCGAGCTCGGGTTATGGAAGAAGGAACCGAGAAGAAAGTATCAGCAACAACAGGTTTTATTACGGGACAACTCATGATG

mRNA sequence

ATGATTTTTAAATCTTTTATACTAGGTAATCTAGTATCCTTCTGCATGAAGATAATCAATTCAGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTCTCCATAGGGCCCTCTTATCTCTTCCTTCTCCGAGCTCGGGTTATGGAAGAAGGAACCGAGAAGAAAGTATCAGCAACAACAGGTTTTATTACGGGACAACTCATGATG

Coding sequence (CDS)

ATGATTTTTAAATCTTTTATACTAGGTAATCTAGTATCCTTCTGCATGAAGATAATCAATTCAGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTCTCCATAGGGCCCTCTTATCTCTTCCTTCTCCGAGCTCGGGTTATGGAAGAAGGAACCGAGAAGAAAGTATCAGCAACAACAGGTTTTATTACGGGACAACTCATGATG

Protein sequence

MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSATTGFITGQLMM

Homology

[Close]

BLAST of MC01g0029 vs. ExPASy Swiss-Prot
Match: A0A393 (Protein TIC 214 OS=Coffea arabica OX=13443 GN=TIC214 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 1.4e-30
Identity = 68/71 (95.77%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIF+SF+LGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFQSFLLGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 72
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy Swiss-Prot
Match: A6MM95 (Protein TIC 214 OS=Buxus microphylla OX=153571 GN=TIC214 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 5.5e-30
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MI KSF+LGNL+S CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MILKSFLLGNLLSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 72
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy Swiss-Prot
Match: A6MMQ5 (Protein TIC 214 OS=Dioscorea elephantipes OX=145284 GN=TIC214 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 9.4e-30
Identity = 66/71 (92.96%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSF+LGNL+S C+KIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEK+VSA
Sbjct: 1  MIFKSFLLGNLLSLCIKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKEVSA 60

Query: 61 TTGFITGQLMM 72
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy Swiss-Prot
Match: Q3C1P6 (Protein TIC 214 OS=Nicotiana sylvestris OX=4096 GN=TIC214 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 9.4e-30
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIF+SF+LGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRA VMEEGTEKKVSA
Sbjct: 1  MIFQSFLLGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRALVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 72
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy Swiss-Prot
Match: Q33BW4 (Protein TIC 214 OS=Nicotiana tomentosiformis OX=4098 GN=TIC214 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 9.4e-30
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIF+SF+LGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRA VMEEGTEKKVSA
Sbjct: 1  MIFQSFLLGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRALVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 72
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. NCBI nr
Match: OWK25800.1 (hypothetical protein AJ87_01050 [Rhizobium yanglingense])

HSP 1 Score: 134 bits (336), Expect = 1.40e-38
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSFILGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFKSFILGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. NCBI nr
Match: AEK71575.1 (hypothetical chloroplast RF1, partial [Spiraea tomentosa])

HSP 1 Score: 134 bits (336), Expect = 4.89e-37
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSFILGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFKSFILGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. NCBI nr
Match: WP_131796261.1 (hypothetical protein, partial [Candidatus Frankia datiscae])

HSP 1 Score: 133 bits (335), Expect = 5.20e-37
Identity = 69/71 (97.18%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSFILGNLVS CMKIINSVVV+GLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFKSFILGNLVSLCMKIINSVVVIGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. NCBI nr
Match: YP_009706462.1 (Ycf1 [Styphnolobium japonicum var. japonicum] >AYM32459.1 Ycf1 [Styphnolobium japonicum var. japonicum])

HSP 1 Score: 129 bits (324), Expect = 8.61e-37
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIF+SFIL NLVS CMKIINS+VVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFQSFILDNLVSLCMKIINSIVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. NCBI nr
Match: GFP97092.1 (protein tic 214 [Phtheirospermum japonicum])

HSP 1 Score: 128 bits (322), Expect = 1.84e-36
Identity = 66/71 (92.96%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIF+SF+LGNLVS C+KIINSVVVVGLYYGFLTTFSIGPSYLFLLRA+VMEEGTEKKVSA
Sbjct: 1  MIFQSFLLGNLVSLCIKIINSVVVVGLYYGFLTTFSIGPSYLFLLRAQVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy TrEMBL
Match: A0A245ZAB5 (Uncharacterized protein OS=Rhizobium yanglingense OX=61013 GN=AJ87_01050 PE=4 SV=1)

HSP 1 Score: 134 bits (336), Expect = 6.77e-39
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSFILGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFKSFILGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy TrEMBL
Match: G0YD98 (Hypothetical chloroplast RF1 (Fragment) OS=Spiraea tomentosa OX=473049 GN=ycf1 PE=4 SV=1)

HSP 1 Score: 134 bits (336), Expect = 2.37e-37
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSFILGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFKSFILGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy TrEMBL
Match: A0A5K6W7Y8 (Protein TIC 214 OS=Styphnolobium japonicum var. japonicum OX=2479786 GN=ycf1 PE=3 SV=1)

HSP 1 Score: 129 bits (324), Expect = 4.17e-37
Identity = 67/71 (94.37%), Postives = 69/71 (97.18%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIF+SFIL NLVS CMKIINS+VVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA
Sbjct: 1  MIFQSFILDNLVSLCMKIINSIVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy TrEMBL
Match: V9ZBS9 (Protein TIC 214 (Fragment) OS=Hamamelis mollis OX=4396 GN=ycf1 PE=3 SV=1)

HSP 1 Score: 131 bits (330), Expect = 1.04e-36
Identity = 68/71 (95.77%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSF+LGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRA+VMEEGTEKKVSA
Sbjct: 1  MIFKSFLLGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRAQVMEEGTEKKVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. ExPASy TrEMBL
Match: A0A6G9L6S8 (Protein TIC 214 OS=Gleditsia sinensis OX=66096 GN=ycf1 PE=3 SV=1)

HSP 1 Score: 129 bits (324), Expect = 1.11e-36
Identity = 68/71 (95.77%), Postives = 68/71 (95.77%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKKVSA 60
          MIFKSFIL NLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEK VSA
Sbjct: 1  MIFKSFILDNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMEEGTEKNVSA 60

Query: 61 TTGFITGQLMM 71
          TTGFITGQLMM
Sbjct: 61 TTGFITGQLMM 71

[Close]

BLAST of MC01g0029 vs. TAIR 10
Match: ATCG01130.1 (Ycf1 protein )

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-29
Identity = 67/74 (90.54%), Postives = 69/74 (93.24%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM---EEGTEKK 60
          M+F+SFILGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM   EEGTEKK
Sbjct: 2  MVFQSFILGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMDEGEEGTEKK 61

Query: 61 VSATTGFITGQLMM 72
          VSATTGFI GQLMM
Sbjct: 62 VSATTGFIAGQLMM 75

[Close]

BLAST of MC01g0029 vs. TAIR 10
Match: ATCG01000.1 (Ycf1 protein )

HSP 1 Score: 125.9 bits (315), Expect = 1.3e-29
Identity = 67/74 (90.54%), Postives = 69/74 (93.24%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM---EEGTEKK 60
          M+F+SFILGNLVS CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM   EEGTEKK
Sbjct: 2  MVFQSFILGNLVSLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMDEGEEGTEKK 61

Query: 61 VSATTGFITGQLMM 72
          VSATTGFI GQLMM
Sbjct: 62 VSATTGFIAGQLMM 75

[Close]

BLAST of MC01g0029 vs. TAIR 10
Match: ATMG00370.1 (Ycf1 protein )

HSP 1 Score: 123.6 bits (309), Expect = 6.2e-29
Identity = 66/74 (89.19%), Postives = 68/74 (91.89%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM---EEGTEKK 60
          M+F+SFILGNLV  CMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM   EEGTEKK
Sbjct: 1  MVFQSFILGNLVYLCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVMDEGEEGTEKK 60

Query: 61 VSATTGFITGQLMM 72
          VSATTGFI GQLMM
Sbjct: 61 VSATTGFIAGQLMM 74

[Close]

BLAST of MC01g0029 vs. TAIR 10
Match: AT2G07739.1 (Ycf1 protein )

HSP 1 Score: 121.3 bits (303), Expect = 3.1e-28
Identity = 65/74 (87.84%), Postives = 67/74 (90.54%), Query Frame = 0


Query: 1  MIFKSFILGNLVSFCMKIINSVVVVGLYYGFLTTFSIGPSYLFLLRARVM---EEGTEKK 60
          M+F+SFILGNLV  CMKIINSVVVVGLYYGFLTTFSIGP YLFLLRARVM   EEGTEKK
Sbjct: 1  MVFQSFILGNLVYLCMKIINSVVVVGLYYGFLTTFSIGPFYLFLLRARVMDEGEEGTEKK 60

Query: 61 VSATTGFITGQLMM 72
          VSATTGFI GQLMM
Sbjct: 61 VSATTGFIAGQLMM 74

The following BLAST results are available for this feature:

BLAST of MC01g0029 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A393	1.4e-30	95.77	Protein TIC 214 OS=Coffea arabica OX=13443 GN=TIC214 PE=3 SV=1	[more]
A6MM95	5.5e-30	94.37	Protein TIC 214 OS=Buxus microphylla OX=153571 GN=TIC214 PE=3 SV=1	[more]
A6MMQ5	9.4e-30	92.96	Protein TIC 214 OS=Dioscorea elephantipes OX=145284 GN=TIC214 PE=3 SV=1	[more]
Q3C1P6	9.4e-30	94.37	Protein TIC 214 OS=Nicotiana sylvestris OX=4096 GN=TIC214 PE=3 SV=1	[more]
Q33BW4	9.4e-30	94.37	Protein TIC 214 OS=Nicotiana tomentosiformis OX=4098 GN=TIC214 PE=3 SV=1	[more]

BLAST of MC01g0029 vs. NCBI nr


Match Name	E-value	Identity	Description
OWK25800.1	1.40e-38	98.59	hypothetical protein AJ87_01050 [Rhizobium yanglingense]	[more]
AEK71575.1	4.89e-37	98.59	hypothetical chloroplast RF1, partial [Spiraea tomentosa]	[more]
WP_131796261.1	5.20e-37	97.18	hypothetical protein, partial [Candidatus Frankia datiscae]	[more]
YP_009706462.1	8.61e-37	94.37	Ycf1 [Styphnolobium japonicum var. japonicum] >AYM32459.1 Ycf1 [Styphnolobium ja...	[more]
GFP97092.1	1.84e-36	92.96	protein tic 214 [Phtheirospermum japonicum]	[more]

BLAST of MC01g0029 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A245ZAB5	6.77e-39	98.59	Uncharacterized protein OS=Rhizobium yanglingense OX=61013 GN=AJ87_01050 PE=4 SV...	[more]
G0YD98	2.37e-37	98.59	Hypothetical chloroplast RF1 (Fragment) OS=Spiraea tomentosa OX=473049 GN=ycf1 P...	[more]
A0A5K6W7Y8	4.17e-37	94.37	Protein TIC 214 OS=Styphnolobium japonicum var. japonicum OX=2479786 GN=ycf1 PE=...	[more]
V9ZBS9	1.04e-36	95.77	Protein TIC 214 (Fragment) OS=Hamamelis mollis OX=4396 GN=ycf1 PE=3 SV=1	[more]
A0A6G9L6S8	1.11e-36	95.77	Protein TIC 214 OS=Gleditsia sinensis OX=66096 GN=ycf1 PE=3 SV=1	[more]

BLAST of MC01g0029 vs. TAIR 10


Match Name	E-value	Identity	Description
ATCG01130.1	1.3e-29	90.54	Ycf1 protein	[more]
ATCG01000.1	1.3e-29	90.54	Ycf1 protein	[more]
ATMG00370.1	6.2e-29	89.19	Ycf1 protein	[more]
AT2G07739.1	3.1e-28	87.84	Ycf1 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008896	Protein TIC214	PFAM	PF05758	Ycf1	coord: 21..71 e-value: 2.6E-29 score: 101.8
IPR008896	Protein TIC214	PANTHER	PTHR33163	PROTEIN TIC 214-RELATED	coord: 1..71
None	No IPR available	PANTHER	PTHR33163:SF13	PROTEIN TIC 214-RELATED	coord: 1..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0029.1	MC01g0029.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015031	protein transport
cellular_component	GO:0009706	chloroplast inner membrane
cellular_component	GO:0016021	integral component of membrane