MC01g0020 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC01g0020
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Ribonuclease T(2)
Location	MC01: 1618878 .. 1619126 (+)
RNA-Seq Expression	MC01g0020
Synteny	MC01g0020

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGATATGATTAATGGTCTCGACCTTCTTGCTACCTGCAGACTTGCTAGGCTAGGGTCAAACTATAACACGAAGACAGTAGCAAGCGTACGATACGCAATCAAAATGCAATTCGGGACCGAACCAGTTCTTCACAACAAAATAGCTACTACACAAACCCTATTAGTGGAAATTGTGATGTGTTTCGATGTAGATGGTGTCACTCTTATTGATTTTGATCCTTCCAAATCCAATTGTGGTTACTCGTTC

mRNA sequence

ATAGATATGATTAATGGTCTCGACCTTCTTGCTACCTGCAGACTTGCTAGGCTAGGGTCAAACTATAACACGAAGACAGTAGCAAGCGTACGATACGCAATCAAAATGCAATTCGGGACCGAACCAGTTCTTCACAACAAAATAGCTACTACACAAACCCTATTAGTGGAAATTGTGATGTGTTTCGATGTAGATGGTGTCACTCTTATTGATTTTGATCCTTCCAAATCCAATTGTGGTTACTCGTTC

Coding sequence (CDS)

ATAGATATGATTAATGGTCTCGACCTTCTTGCTACCTGCAGACTTGCTAGGCTAGGGTCAAACTATAACACGAAGACAGTAGCAAGCGTACGATACGCAATCAAAATGCAATTCGGGACCGAACCAGTTCTTCACAACAAAATAGCTACTACACAAACCCTATTAGTGGAAATTGTGATGTGTTTCGATGTAGATGGTGTCACTCTTATTGATTTTGATCCTTCCAAATCCAATTGTGGTTACTCGTTC

Protein sequence

IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVMCFDVDGVTLIDFDPSKSNCGYSF

Homology

[Close]

BLAST of MC01g0020 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 7.1e-05
Identity = 29/85 (34.12%), Postives = 45/85 (52.94%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKI--ATTQTLLVEI 60
           +DM N  D++   R    G N  TK+  +++  +K +FG  P L  +    T  + LV++
Sbjct: 107 VDMRNNYDIIGALRPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQV 166

Query: 61  VMCFDVDGVTLIDFDPSKSNCGYSF 84
           V CF  DG TLID   ++  CG +F
Sbjct: 167 VACFAQDGSTLIDC--TRDTCGANF 189

[Close]

BLAST of MC01g0020 vs. NCBI nr
Match: XP_022142280.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 75.9 bits (185), Expect = 1.64e-14
Identity = 40/83 (48.19%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVM 60
           I+M + LDL+   R+  L  N  TK+   V+ AI+ QFG EPVL  +       L+EIVM
Sbjct: 136 INMRDSLDLMNALRVGGLAPNGRTKSRQRVQSAIRAQFGKEPVLRCRGTGRNVRLIEIVM 195

Query: 61  CFDVDGVTLIDFDPSKSNCGYSF 83
           CFD DGVTLI+ +P+ SNC  SF
Sbjct: 196 CFDDDGVTLINCNPASSNCANSF 218

[Close]

BLAST of MC01g0020 vs. NCBI nr
Match: BAA10891.1 (ribonuclease (RNase LC1) [Luffa aegyptiaca])

HSP 1 Score: 73.2 bits (178), Expect = 1.60e-13
Identity = 39/83 (46.99%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVM 60
           I+M N +DLL+  R+  +  N  +K    V+ AI+ Q G EPVL  +    Q+ L+EIVM
Sbjct: 130 INMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCRGTGRQSRLLEIVM 189

Query: 61  CFDVDGVTLIDFDPSKSNCGYSF 83
           CFD DGVTLI+ +P+ SNC  SF
Sbjct: 190 CFDDDGVTLINCNPANSNCPNSF 212

[Close]

BLAST of MC01g0020 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 73.2 bits (178), Expect = 1.68e-13
Identity = 40/84 (47.62%), Postives = 53/84 (63.10%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATT-QTLLVEIV 60
           I+M +G+DLL+  R   +G N  +K    V  AI   FG +P+L  K A+  Q LL EIV
Sbjct: 132 INMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEIV 191

Query: 61  MCFDVDGVTLIDFDPSKSNCGYSF 83
           MCFD DGVTLI+ + ++SNC  SF
Sbjct: 192 MCFDDDGVTLINCNKARSNCAGSF 215

[Close]

BLAST of MC01g0020 vs. NCBI nr
Match: XP_022155091.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 2.47e-12
Identity = 36/83 (43.37%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVM 60
           I+M N +DL+   ++  L  N  +K+   V+ A++  FG EPVL  +   +   L+EIVM
Sbjct: 132 INMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVM 191

Query: 61  CFDVDGVTLIDFDPSKSNCGYSF 83
           CFD DGVTLI+ +P  SNC  SF
Sbjct: 192 CFDDDGVTLINCNPVSSNCANSF 214

[Close]

BLAST of MC01g0020 vs. NCBI nr
Match: KAA0053769.1 (ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25633.1 ribonuclease MC-like [Cucumis melo var. makuwa])

HSP 1 Score: 65.1 bits (157), Expect = 5.89e-11
Identity = 36/84 (42.86%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATT-QTLLVEIV 60
           I+M + +DLL+  R   +  N  +K    V  AI   F  +P+L  K A+  Q LL EIV
Sbjct: 61  INMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQVLLTEIV 120

Query: 61  MCFDVDGVTLIDFDPSKSNCGYSF 83
           +CFD DGVTLI+ + ++SNC  SF
Sbjct: 121 ICFDDDGVTLINCNKARSNCARSF 144

[Close]

BLAST of MC01g0020 vs. ExPASy TrEMBL
Match: A0A6J1CKH7 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 7.95e-15
Identity = 40/83 (48.19%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVM 60
           I+M + LDL+   R+  L  N  TK+   V+ AI+ QFG EPVL  +       L+EIVM
Sbjct: 136 INMRDSLDLMNALRVGGLAPNGRTKSRQRVQSAIRAQFGKEPVLRCRGTGRNVRLIEIVM 195

Query: 61  CFDVDGVTLIDFDPSKSNCGYSF 83
           CFD DGVTLI+ +P+ SNC  SF
Sbjct: 196 CFDDDGVTLINCNPASSNCANSF 218

[Close]

BLAST of MC01g0020 vs. ExPASy TrEMBL
Match: Q40115 (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.73e-14
Identity = 39/83 (46.99%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVM 60
           I+M N +DLL+  R+  +  N  +K    V+ AI+ Q G EPVL  +    Q+ L+EIVM
Sbjct: 130 INMRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCRGTGRQSRLLEIVM 189

Query: 61  CFDVDGVTLIDFDPSKSNCGYSF 83
           CFD DGVTLI+ +P+ SNC  SF
Sbjct: 190 CFDDDGVTLINCNPANSNCPNSF 212

[Close]

BLAST of MC01g0020 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 8.12e-14
Identity = 40/84 (47.62%), Postives = 53/84 (63.10%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATT-QTLLVEIV 60
           I+M +G+DLL+  R   +G N  +K    V  AI   FG +P+L  K A+  Q LL EIV
Sbjct: 132 INMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEIV 191

Query: 61  MCFDVDGVTLIDFDPSKSNCGYSF 83
           MCFD DGVTLI+ + ++SNC  SF
Sbjct: 192 MCFDDDGVTLINCNKARSNCAGSF 215

[Close]

BLAST of MC01g0020 vs. ExPASy TrEMBL
Match: A0A6J1DQN7 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.20e-12
Identity = 36/83 (43.37%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATTQTLLVEIVM 60
           I+M N +DL+   ++  L  N  +K+   V+ A++  FG EPVL  +   +   L+EIVM
Sbjct: 132 INMRNSIDLMNALKVGGLAPNGLSKSKQRVQSALRTHFGKEPVLRCRGTGSNIRLIEIVM 191

Query: 61  CFDVDGVTLIDFDPSKSNCGYSF 83
           CFD DGVTLI+ +P  SNC  SF
Sbjct: 192 CFDDDGVTLINCNPVSSNCANSF 214

[Close]

BLAST of MC01g0020 vs. ExPASy TrEMBL
Match: A0A5A7UJY4 (Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007930 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.85e-11
Identity = 36/84 (42.86%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 1   IDMINGLDLLATCRLARLGSNYNTKTVASVRYAIKMQFGTEPVLHNKIATT-QTLLVEIV 60
           I+M + +DLL+  R   +  N  +K    V  AI   F  +P+L  K A+  Q LL EIV
Sbjct: 61  INMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQVLLTEIV 120

Query: 61  MCFDVDGVTLIDFDPSKSNCGYSF 83
           +CFD DGVTLI+ + ++SNC  SF
Sbjct: 121 ICFDDDGVTLINCNKARSNCARSF 144

The following BLAST results are available for this feature:

BLAST of MC01g0020 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	7.1e-05	34.12	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]

BLAST of MC01g0020 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142280.1	1.64e-14	48.19	ribonuclease MC-like [Momordica charantia]	[more]
BAA10891.1	1.60e-13	46.99	ribonuclease (RNase LC1) [Luffa aegyptiaca]	[more]
XP_004147499.1	1.68e-13	47.62	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_022155091.1	2.47e-12	43.37	ribonuclease MC-like [Momordica charantia]	[more]
KAA0053769.1	5.89e-11	42.86	ribonuclease MC-like [Cucumis melo var. makuwa] >TYK25633.1 ribonuclease MC-like...	[more]

BLAST of MC01g0020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKH7	7.95e-15	48.19	Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111012440 PE=3 SV=1	[more]
Q40115	7.73e-14	46.99	Ribonuclease (RNase LC1) OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A0A0LC37	8.12e-14	47.62	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A6J1DQN7	1.20e-12	43.37	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111022224 PE=3 SV=1	[more]
A0A5A7UJY4	2.85e-11	42.86	Ribonuclease MC-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352...	[more]

BLAST of MC01g0020 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 1..83 e-value: 4.0E-6 score: 29.0
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 2..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC01g0020.1	MC01g0020.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016070	RNA metabolic process
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding