MC00g_new0687 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g_new0687
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	RNase H domain-containing protein
Location	scaffold31: 1022151 .. 1022988 (-)
RNA-Seq Expression	MC00g_new0687
Synteny	MC00g_new0687

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGGAATGACAGAAATGCAATTCGGGCCTAAAAACAAATACTTGATGTCAGGAGCAAATGCGAATGGATTCCTTCTCATATGAAGGAAATTAATTCTAGTTTTCAGGCACATCTGTAATATCTGGTCTCCACCTCCCCTGGGGTGGGTTAAATTTCATGTTGATGCGGCGTGTAATCCGGCGACTCCTTGTATTGGTATAGGAGCTATTTGTCGGAACAGTGAAGGGGAAATTTTGGTTACCATGTCCAAAGGCTTCGATGTTCACTACGATCCCCTAACAGTGGAATTACTCGCCATCCGTGAATGTCTGAAGCTCGCTCTTCAGTTAAATTATCGATCTGTGCAGCTGGAGTCGGACTGTCTTCAAGCTATTGAGCTTCACAAAGGGGACAAAGCGATTTGGTTGGAGCCTGGCACCTGGGTTGAGGATATCAGAAATTCCATGATACATTTCACCCCGATTTCATTCTTGCATGTGAGAAGAGAAGGCAATAGGGTTGCCCACAAGCTCGCGTCGAATGTGGTTGCCTCCGTGGGTTTTTGTGTCTGTTCTTCTAATTTCCCAATTTAGACTATTAATAACTCTATAAGGGACTGTAATAGCCTTTTAGGCTTTGCGTTAGTTTTCTAATAAAAGATATAAATATTGTGCGGACTGCATTAATGCCGTCTACACACCATGCTGCAGTTTATATGCAAGATATGACCACGGGTCCTACAATCCTCCTTCCTTTCTTCCACTTTCTTCTTCCTTTCGTTTTCTACTTCCTCCGGCTCTTCTTCTCTCGATCTCATTTTTGTTATTCTATTTTTCTCTTAGATTTCTCGATTTCTT

mRNA sequence

ATGTCCAAAGGCTTCGATGTTCACTACGATCCCCTAACAGTGGAATTACTCGCCATCCGTGAATGTCTGAAGCTCGCTCTTCAGTTAAATTATCGATCTGTGCAGCTGGAGTCGGACTGTCTTCAAGCTATTGAGCTTCACAAAGGGGACAAAGCGATTTGGTTGGAGCCTGGCACCTGGGTTGAGGATATCAGAAATTCCATGATACATTTCACCCCGATTTCATTCTTGCATGTGAGAAGAGAAGGCAATAGGGTTGCCCACAAGCTCGCGTCGAATGTGGTTGCCTCCGTGGGTTTTTGTGTCTGTTCTTCTAATTTCCCAATTTAG

Coding sequence (CDS)

ATGTCCAAAGGCTTCGATGTTCACTACGATCCCCTAACAGTGGAATTACTCGCCATCCGTGAATGTCTGAAGCTCGCTCTTCAGTTAAATTATCGATCTGTGCAGCTGGAGTCGGACTGTCTTCAAGCTATTGAGCTTCACAAAGGGGACAAAGCGATTTGGTTGGAGCCTGGCACCTGGGTTGAGGATATCAGAAATTCCATGATACATTTCACCCCGATTTCATTCTTGCATGTGAGAAGAGAAGGCAATAGGGTTGCCCACAAGCTCGCGTCGAATGTGGTTGCCTCCGTGGGTTTTTGTGTCTGTTCTTCTAATTTCCCAATTTAG

Protein sequence

MSKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWVEDIRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFPI

Homology

[Close]

BLAST of MC00g_new0687 vs. NCBI nr
Match: XP_022142956.1 (uncharacterized protein LOC111012950 [Momordica charantia])

HSP 1 Score: 73.2 bits (178), Expect = 1.97e-13
Identity = 40/94 (42.55%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 2   SKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWV 61
           S+  + ++DPL  E LA+ E L+LA  LN + VQ ESD LQ I L   D   W     W+
Sbjct: 66  SRRMEAYHDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWL 125

Query: 62  EDIRNSMIHFTPISFLHVRREGNRVAHKLASNVV 95
           EDIR    +F   +FLHV R  N+ A ++AS  V
Sbjct: 126 EDIRVLARNFIDFNFLHVCRSSNKAARRVASFAV 159

[Close]

BLAST of MC00g_new0687 vs. NCBI nr
Match: XP_022154440.1 (uncharacterized protein LOC111021711 [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 1.42e-11
Identity = 41/108 (37.96%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 1   MSKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTW 60
           M +  DV Y+PL VE LAI E L L   L+   VQ++SD   +I L   +     +P  W
Sbjct: 168 MIRKLDVFYEPLQVETLAILEGLWLTQCLDLIFVQVDSDSQNSINLISQEYDPSHQPNVW 227

Query: 61  VEDIRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFP 108
           V+DI    I+F  I F+H++R  + V H+LAS  ++S    +  S FP
Sbjct: 228 VDDIHMCAINFIDILFMHIQRSSSNVTHRLASTAISSNPNKIWISRFP 275

[Close]

BLAST of MC00g_new0687 vs. NCBI nr
Match: KAG8388125.1 (hypothetical protein BUALT_Bualt02G0093200 [Buddleja alternifolia])

HSP 1 Score: 66.6 bits (161), Expect = 9.11e-11
Identity = 36/97 (37.11%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 1   MSKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTW 60
           +SK F   +DP   E LA  E ++L++++ +R + LE DCLQ I   +          + 
Sbjct: 89  LSKFFSAIHDPKRAEALAAHEAMELSIRMGWREIILEEDCLQVISKLRSPNTNSSFISSL 148

Query: 61  VEDIRNSMIHFTPISFLHVRREGNRVAHKLASNVVAS 97
           V DI+  + +F  +SF HV+R GNRVAH LA    +S
Sbjct: 149 VSDIKCLVSNFICVSFSHVKRAGNRVAHSLARLATSS 185

[Close]

BLAST of MC00g_new0687 vs. NCBI nr
Match: AFP55557.1 (non-ltr retroelement reverse transcriptase [Rosa rugosa])

HSP 1 Score: 66.6 bits (161), Expect = 4.90e-10
Identity = 39/98 (39.80%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 2    SKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWV 61
             K F      L  ELLAI+  L L ++   R++ +ESDCL+AI L    +      G  V
Sbjct: 1531 GKNFQHPVSSLVAELLAIKVGLDLVVERRLRNIMVESDCLEAIHLLNSKERCLAPEGGLV 1590

Query: 62   EDIRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVG 99
            EDI+N+M      S  HVRREGN  AH +A  V  + G
Sbjct: 1591 EDIQNTMALVNISSIYHVRREGNTAAHAIAKFVARNNG 1628

[Close]

BLAST of MC00g_new0687 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 62.4 bits (150), Expect = 6.07e-09
Identity = 43/106 (40.57%), Postives = 58/106 (54.72%), Query Frame = 0


Query: 5   FDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWL-EPGTWVED 64
           F +   PL  ++LAIRE L LA +L    V +E+D L+A+ L  GDK+ W  E  +WVED
Sbjct: 108 FHMVKTPLLAKILAIREGLSLATRLGVHRVVVETDSLEALNLI-GDKSPWKGEAVSWVED 167

Query: 65  IRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFPI 109
           IR     F  I F HV RE N VA+ L    ++     +   +FPI
Sbjct: 168 IRAFARDFQEICFQHVFRESNAVAYHLGRETISLRCGFLWRLDFPI 212

[Close]

BLAST of MC00g_new0687 vs. ExPASy TrEMBL
Match: A0A6J1CNR4 (uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012950 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 9.55e-14
Identity = 40/94 (42.55%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 2   SKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWV 61
           S+  + ++DPL  E LA+ E L+LA  LN + VQ ESD LQ I L   D   W     W+
Sbjct: 66  SRRMEAYHDPLMAESLALLEGLRLADHLNIQQVQFESDSLQLINLLTEDPISWCGSNVWL 125

Query: 62  EDIRNSMIHFTPISFLHVRREGNRVAHKLASNVV 95
           EDIR    +F   +FLHV R  N+ A ++AS  V
Sbjct: 126 EDIRVLARNFIDFNFLHVCRSSNKAARRVASFAV 159

[Close]

BLAST of MC00g_new0687 vs. ExPASy TrEMBL
Match: A0A6J1DJL6 (uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021711 PE=4 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 6.86e-12
Identity = 41/108 (37.96%), Postives = 59/108 (54.63%), Query Frame = 0


Query: 1   MSKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTW 60
           M +  DV Y+PL VE LAI E L L   L+   VQ++SD   +I L   +     +P  W
Sbjct: 168 MIRKLDVFYEPLQVETLAILEGLWLTQCLDLIFVQVDSDSQNSINLISQEYDPSHQPNVW 227

Query: 61  VEDIRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFP 108
           V+DI    I+F  I F+H++R  + V H+LAS  ++S    +  S FP
Sbjct: 228 VDDIHMCAINFIDILFMHIQRSSSNVTHRLASTAISSNPNKIWISRFP 275

[Close]

BLAST of MC00g_new0687 vs. ExPASy TrEMBL
Match: J7G0Q7 (Non-ltr retroelement reverse transcriptase OS=Rosa rugosa OX=74645 PE=4 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 2.37e-10
Identity = 39/98 (39.80%), Postives = 51/98 (52.04%), Query Frame = 0


Query: 2    SKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWV 61
             K F      L  ELLAI+  L L ++   R++ +ESDCL+AI L    +      G  V
Sbjct: 1531 GKNFQHPVSSLVAELLAIKVGLDLVVERRLRNIMVESDCLEAIHLLNSKERCLAPEGGLV 1590

Query: 62   EDIRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVG 99
            EDI+N+M      S  HVRREGN  AH +A  V  + G
Sbjct: 1591 EDIQNTMALVNISSIYHVRREGNTAAHAIAKFVARNNG 1628

[Close]

BLAST of MC00g_new0687 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.94e-09
Identity = 43/106 (40.57%), Postives = 58/106 (54.72%), Query Frame = 0


Query: 5   FDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWL-EPGTWVED 64
           F +   PL  ++LAIRE L LA +L    V +E+D L+A+ L  GDK+ W  E  +WVED
Sbjct: 108 FHMVKTPLLAKILAIREGLSLATRLGVHRVVVETDSLEALNLI-GDKSPWKGEAVSWVED 167

Query: 65  IRNSMIHFTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFPI 109
           IR     F  I F HV RE N VA+ L    ++     +   +FPI
Sbjct: 168 IRAFARDFQEICFQHVFRESNAVAYHLGRETISLRCGFLWRLDFPI 212

[Close]

BLAST of MC00g_new0687 vs. ExPASy TrEMBL
Match: A0A2I4DVZ3 (uncharacterized protein LOC108983971 OS=Juglans regia OX=51240 GN=LOC108983971 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 1.10e-08
Identity = 31/90 (34.44%), Postives = 51/90 (56.67%), Query Frame = 0


Query: 2   SKGFDVHYDPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWV 61
           +K  D    P+T+EL+A+   L+L   L   ++ +E+DCL  ++  +G +  +   G  +
Sbjct: 82  TKAEDAVDAPVTIELIAVLRGLQLCQPLGIPNLVVETDCLSLVQELQGTQESFSPNGNLI 141

Query: 62  EDIRNSMIHFTPISFLHVRREGNRVAHKLA 91
           +D +  M HF  +   HV R+GNRVAH LA
Sbjct: 142 KDAKELMRHFPELQVQHVYRDGNRVAHNLA 171

[Close]

BLAST of MC00g_new0687 vs. TAIR 10
Match: AT5G42965.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 8.7e-06
Identity = 27/89 (30.34%), Postives = 45/89 (50.56%), Query Frame = 0


Query: 12  LTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWVEDIRNSMIHF 71
           L VEL A+R       + NY+ V  ESD    + L  GD   +      ++DIR+    F
Sbjct: 16  LEVELEAMRWAALSMSRFNYKKVIFESDSQHLVSL-LGDDTDYPNLAPRIQDIRHIFQQF 75

Query: 72  TPISFLHVRREGNRVAHKLASNVVASVGF 101
             +  +H++REGN VA ++A   ++ + +
Sbjct: 76  EEVKVMHIKREGNSVADRIARESLSLLNY 103

[Close]

BLAST of MC00g_new0687 vs. TAIR 10
Match: AT2G13980.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 4.8e-04
Identity = 32/99 (32.32%), Postives = 42/99 (42.42%), Query Frame = 0


Query: 11  PLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWVEDIRNSMIH 70
           PL  E  A+   L+      Y  V +E DC     L  G  +        ++DIR     
Sbjct: 63  PLEAETKALLAALQQTWIRGYLRVIMEGDCETLTNLVSGSSS-HNHLANLLDDIRIWAKK 122

Query: 71  FTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFPI 110
           F+ + F  VRR GN+VAH+LA     S  F   S   PI
Sbjct: 123 FSNVQFSFVRRGGNKVAHELAKLGCNSTCFYSDSCTLPI 160

[Close]

BLAST of MC00g_new0687 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 40.8 bits (94), Expect = 8.1e-04
Identity = 31/100 (31.00%), Postives = 42/100 (42.00%), Query Frame = 0


Query: 10  DPLTVELLAIRECLKLALQLNYRSVQLESDCLQAIELHKGDKAIWLEPGTWVEDIRNSMI 69
           +PL  E  A+   L+      Y  V +E DC   I L  G  +        +EDI     
Sbjct: 375 NPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLING-ISFHSSLANHLEDISFWAN 434

Query: 70  HFTPISFLHVRREGNRVAHKLASNVVASVGFCVCSSNFPI 110
            F  I F  +RR+GN++AH LA        F   S + PI
Sbjct: 435 KFASIQFGFIRRKGNKLAHVLAKYGCTYSTFYSGSGSLPI 473

The following BLAST results are available for this feature:

BLAST of MC00g_new0687 vs.


Match Name	E-value	Identity	Description

BLAST of MC00g_new0687 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142956.1	1.97e-13	42.55	uncharacterized protein LOC111012950 [Momordica charantia]	[more]
XP_022154440.1	1.42e-11	37.96	uncharacterized protein LOC111021711 [Momordica charantia]	[more]
KAG8388125.1	9.11e-11	37.11	hypothetical protein BUALT_Bualt02G0093200 [Buddleja alternifolia]	[more]
AFP55557.1	4.90e-10	39.80	non-ltr retroelement reverse transcriptase [Rosa rugosa]	[more]
XP_022158489.1	6.07e-09	40.57	uncharacterized protein LOC111024968 [Momordica charantia]	[more]

BLAST of MC00g_new0687 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CNR4	9.55e-14	42.55	uncharacterized protein LOC111012950 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DJL6	6.86e-12	37.96	uncharacterized protein LOC111021711 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
J7G0Q7	2.37e-10	39.80	Non-ltr retroelement reverse transcriptase OS=Rosa rugosa OX=74645 PE=4 SV=1	[more]
A0A6J1DZK3	2.94e-09	40.57	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A2I4DVZ3	1.10e-08	34.44	uncharacterized protein LOC108983971 OS=Juglans regia OX=51240 GN=LOC108983971 P...	[more]

BLAST of MC00g_new0687 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42965.1	8.7e-06	30.34	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G13980.1	4.8e-04	32.32	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G09510.1	8.1e-04	31.00	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 5..92 e-value: 1.5E-18 score: 66.8
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 2..98 e-value: 5.4E-10 score: 41.3
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 8..92
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 8..92
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 8..91 e-value: 2.21643E-19 score: 74.658
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 9..95

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g_new0687.1	MC00g_new0687.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity