MC00g_new0044 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g_new0044
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(-)-germacrene D synthase-like isoform X1
Location	scaffold138: 506088 .. 506991 (+)
RNA-Seq Expression	MC00g_new0044
Synteny	MC00g_new0044

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGGCAATGCAAAGCTTATTTAGTGGAAGCTAAGTGGTTCCATGAAGGACATGTGCCAAGTATGGAAGAATATATGGAAGTTGCCATTGTATCAGCTGCCTACTATCTTTTTGCTTCTGTTTCCATCCTTGGCATGGGAGATGTTGCTTCCAAAGAGGCTTTTCAATGGGTTATGAGTCATATGCTTCTTAATGCAAACGGAGTCATTGGTAGGCTCGTCAATGACATAGCTTCCCACAAGGTACACAAGAAAGAAAAAATTAACCATATGATCAAGTTTGTAATTTAACCAAAGTGTCATCTTTATTCGTGTGCTTGCGAGTGTGGTGGGGTGGAATTCGAGGATGGGTGTTGATATCCAATTCCTAGATTTGAATCCCAAGGGGCGTAGTCTGGGTGGCGACACCCAGTGTCCAGATTGAGTCCTTTGCTGTCGATTACACTGTTAGGTGATATCCATATCTATTGCCCCAAGTTATAGAGATTCTCTGGCGAAGTCACACGACATCTATTCTCTATTATGGAAAAAAAATCTTACTCTCTATTTGAAAGAGTTTTTTTCAAAATTACATTGTAAAACCTTGTTCTTTTTGCATTTTCTCTAGTTTGAACAAGAGAGAGGTCATGTTACTTGTGCGGTTGAATGCTACCTCAAGCAATATGGAGTATCAGAAGAAGAGGTTGTAGGTGAACTAGAAAAGCAAGTCATAAAAGCATGGAAAGATAAAACAGAAGACTACATGAAATCTACCAGATTTGCAAATGCGATCCTTCCATGCGTGACGAATGTTGCTCGACTCTCGGATCTCTTTTATAAGGAGGGAGATGGTTACACATTCCCTCATGGTGAAACAAGACGTTTTATTACCTCAATCCTTAAGGATCGTGTGCCTCTTTAA

mRNA sequence

ATGAAAAGGCAATGCAAAGCTTATTTAGTGGAAGCTAAGTGGTTCCATGAAGGACATGTGCCAAGTATGGAAGAATATATGGAAGTTGCCATTGTATCAGCTGCCTACTATCTTTTTGCTTCTGTTTCCATCCTTGGCATGGGAGATGTTGCTTCCAAAGAGGCTTTTCAATGGGTTATGAGTCATATGCTTCTTAATGCAAACGGAGTCATTGGTAGGCTCGTCAATGACATAGCTTCCCACAAGTTTGAACAAGAGAGAGGTCATGTTACTTGTGCGGTTGAATGCTACCTCAAGCAATATGGAGTATCAGAAGAAGAGGTTGTAGGTGAACTAGAAAAGCAAGTCATAAAAGCATGGAAAGATAAAACAGAAGACTACATGAAATCTACCAGATTTGCAAATGCGATCCTTCCATGCGTGACGAATGTTGCTCGACTCTCGGATCTCTTTTATAAGGAGGGAGATGGTTACACATTCCCTCATGGTGAAACAAGACGTTTTATTACCTCAATCCTTAAGGATCGTGTGCCTCTTTAA

Coding sequence (CDS)

ATGAAAAGGCAATGCAAAGCTTATTTAGTGGAAGCTAAGTGGTTCCATGAAGGACATGTGCCAAGTATGGAAGAATATATGGAAGTTGCCATTGTATCAGCTGCCTACTATCTTTTTGCTTCTGTTTCCATCCTTGGCATGGGAGATGTTGCTTCCAAAGAGGCTTTTCAATGGGTTATGAGTCATATGCTTCTTAATGCAAACGGAGTCATTGGTAGGCTCGTCAATGACATAGCTTCCCACAAGTTTGAACAAGAGAGAGGTCATGTTACTTGTGCGGTTGAATGCTACCTCAAGCAATATGGAGTATCAGAAGAAGAGGTTGTAGGTGAACTAGAAAAGCAAGTCATAAAAGCATGGAAAGATAAAACAGAAGACTACATGAAATCTACCAGATTTGCAAATGCGATCCTTCCATGCGTGACGAATGTTGCTCGACTCTCGGATCTCTTTTATAAGGAGGGAGATGGTTACACATTCCCTCATGGTGAAACAAGACGTTTTATTACCTCAATCCTTAAGGATCGTGTGCCTCTTTAA

Protein sequence

MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVMSHMLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKAWKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL

Homology

[Close]

BLAST of MC00g_new0044 vs. ExPASy Swiss-Prot
Match: Q6Q3H2 (Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.4e-44
Identity = 88/178 (49.44%), Postives = 118/178 (66.29%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MK Q +AY  EAKW HE HVP+ EEYM VA+ S+ Y L A+ S +GMG++A+KEAF WV 
Sbjct: 378 MKNQVRAYFAEAKWLHEEHVPAFEEYMRVALASSGYCLLATTSFVGMGEIATKEAFDWVT 437

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S   +++++  I RL++DI SHKFEQ+RGHVT AVECY+KQYGVSEE+V  E +KQ+  A
Sbjct: 438 SDPKIMSSSNFITRLMDDIKSHKFEQKRGHVTSAVECYMKQYGVSEEQVYSEFQKQIENA 497

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRV 178
           W D  ++ +K T  +  +L  + N  R  D+ YKE D YT      R  I S+  + V
Sbjct: 498 WLDINQECLKPTAVSMPLLARLLNFTRTMDVIYKEQDSYTHVGKVMRDNIASVFINAV 555

[Close]

BLAST of MC00g_new0044 vs. ExPASy Swiss-Prot
Match: F6M8H7 (Probable sesquiterpene synthase OS=Santalum murrayanum OX=453085 GN=STPS PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.8e-43
Identity = 83/178 (46.63%), Postives = 119/178 (66.85%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MK   + Y+ EAKW HE +VP+ +EYM VA+V++ Y    ++S LGMG++ASKEAF W+ 
Sbjct: 383 MKTIVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 442

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           SH  ++ A+  +GRL++D+ SHKFEQERGHV   +ECY+KQYGV+EEE   E  K+++KA
Sbjct: 443 SHPPVIEASESVGRLMDDMRSHKFEQERGHVASGIECYMKQYGVTEEEAHDEFRKRLVKA 502

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRV 178
           WKD  E+ ++  R    +L  + N+ R+ D+ YK  DGYT      +  I S+L D V
Sbjct: 503 WKDINEECLRPYRVPKPLLTRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPV 560

[Close]

BLAST of MC00g_new0044 vs. ExPASy Swiss-Prot
Match: F6M8H5 (Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 6.4e-43
Identity = 82/176 (46.59%), Postives = 118/176 (67.05%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MK+  + Y+ EAKW HE +VP+ +EYM VA+V++ Y    ++S LGMG++ASKEAF W+ 
Sbjct: 383 MKKLVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 442

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           SH  ++ A+  +GRL++D  SHKFEQERGHV   +ECY+KQYGV+EEE   E  K+++KA
Sbjct: 443 SHPPVIEASESVGRLMDDTRSHKFEQERGHVASGIECYMKQYGVTEEEARDEFRKRLVKA 502

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKD 176
           WKD  E+ ++  R    +L  + N+ R+ D+ YK  DGYT      +  I S+L D
Sbjct: 503 WKDINEECLRPYRVPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLID 558

[Close]

BLAST of MC00g_new0044 vs. ExPASy Swiss-Prot
Match: B5A435 (Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 8.4e-43
Identity = 83/176 (47.16%), Postives = 118/176 (67.05%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MK+  + Y+ EAKW HE +VP+ EEYM VA+V++ Y    ++S LGMG++ASKEAF W+ 
Sbjct: 380 MKKLVEGYMDEAKWCHEKYVPTFEEYMPVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 439

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           SH  ++ A+  + RL++D+ SHKFEQERGHV   +ECY+KQYGV+EEE   E  KQ++KA
Sbjct: 440 SHPPVIEASESVCRLMDDMRSHKFEQERGHVASGIECYMKQYGVTEEEAHDEFRKQLVKA 499

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKD 176
           WKD  E+ ++  R    +L  + N+ R+ D+ YK  DGYT      +  I S+L D
Sbjct: 500 WKDINEECLRPYRVPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLID 555

[Close]

BLAST of MC00g_new0044 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.1e-41
Identity = 83/180 (46.11%), Postives = 118/180 (65.56%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MK Q +AY  EAKW     +P+MEEYM VA+V++AY + A+ S +GMGD  +KE+F W+ 
Sbjct: 378 MKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGMGDAVTKESFDWIF 437

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S   ++ A+ ++ RL++D+  HKFEQ+RGHV  AVECY+KQ+G SE+E   E  + V +A
Sbjct: 438 SKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQHGASEQETPNEFPQPVREA 497

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 180
           WKD  E+ +  T     IL  V N+AR+ D+ YK  DGYT      + F+TS+L D VP+
Sbjct: 498 WKDINEECLIPTAVPMPILMRVLNLARVIDVIYKNEDGYTHFGAVLKDFVTSMLIDPVPI 557

[Close]

BLAST of MC00g_new0044 vs. NCBI nr
Match: XP_022143458.1 ((-)-germacrene D synthase-like isoform X3 [Momordica charantia])

HSP 1 Score: 323 bits (827), Expect = 1.21e-106
Identity = 163/180 (90.56%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AYLVEAKWFH+GHVPSMEEYMEVAIVSAAYYLFASVS LGMGDVASKEAFQWVM
Sbjct: 279 MKRQCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVM 338

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  MLLNA GVIGRLVNDIASHKFEQERGHV  AVECY+KQY VSEEE VGELEKQVIKA
Sbjct: 339 SDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKA 398

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD TEDYMKSTRF NAI+ CVTNVARLSDLFYKEGDGYTFP GETRRFITSILKD VPL
Sbjct: 399 WKDITEDYMKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 458

[Close]

BLAST of MC00g_new0044 vs. NCBI nr
Match: XP_022143456.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 323 bits (827), Expect = 2.23e-105
Identity = 163/180 (90.56%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AYLVEAKWFH+GHVPSMEEYMEVAIVSAAYYLFASVS LGMGDVASKEAFQWVM
Sbjct: 379 MKRQCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVM 438

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  MLLNA GVIGRLVNDIASHKFEQERGHV  AVECY+KQY VSEEE VGELEKQVIKA
Sbjct: 439 SDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKA 498

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD TEDYMKSTRF NAI+ CVTNVARLSDLFYKEGDGYTFP GETRRFITSILKD VPL
Sbjct: 499 WKDITEDYMKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 558

[Close]

BLAST of MC00g_new0044 vs. NCBI nr
Match: XP_022143530.1 (valencene synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 276 bits (705), Expect = 5.89e-88
Identity = 136/180 (75.56%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AY  EAKWF+EG+VP+MEEYMEVA +SA+Y+LFA+ S LGMGDVAS E FQW  
Sbjct: 301 MKRQCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAK 360

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  +LL+A GVIGRL+NDI SHKFEQERGHV  AVECY+KQYGVSE E + ELEKQVIKA
Sbjct: 361 SDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKA 420

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD  EDYMK TRF NAIL CV NV RLSD FYKE DGYTFP+GET RFITS+LKD VPL
Sbjct: 421 WKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 480

[Close]

BLAST of MC00g_new0044 vs. NCBI nr
Match: XP_022143529.1 ((-)-germacrene D synthase-like isoform X1 [Momordica charantia])

HSP 1 Score: 276 bits (705), Expect = 4.10e-87
Identity = 136/180 (75.56%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AY  EAKWF+EG+VP+MEEYMEVA +SA+Y+LFA+ S LGMGDVAS E FQW  
Sbjct: 374 MKRQCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAK 433

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  +LL+A GVIGRL+NDI SHKFEQERGHV  AVECY+KQYGVSE E + ELEKQVIKA
Sbjct: 434 SDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKA 493

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD  EDYMK TRF NAIL CV NV RLSD FYKE DGYTFP+GET RFITS+LKD VPL
Sbjct: 494 WKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 553

[Close]

BLAST of MC00g_new0044 vs. NCBI nr
Match: XP_022143532.1 ((-)-germacrene D synthase-like isoform X2 [Momordica charantia])

HSP 1 Score: 265 bits (677), Expect = 1.13e-83
Identity = 129/179 (72.07%), Postives = 150/179 (83.80%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+ YL EAKWF+EG+VP+MEEYME+A +SA+Y+LFA+ S LGMGDV SKE  +W  
Sbjct: 307 MKRQCRVYLAEAKWFYEGYVPTMEEYMEIATISASYHLFAASSFLGMGDVVSKEVLEWAK 366

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  ++L+A+G IGRL+NDI SH+FEQERG V  AVECY+KQYGVSEEE V ELEKQVIKA
Sbjct: 367 SDPIMLSASGDIGRLMNDIVSHEFEQERGDVASAVECYMKQYGVSEEEAVVELEKQVIKA 426

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVP 178
           WKD TEDYMKS +F NAIL CV NV RLSD FYKE DGYTFP+GE +RFITS+LKD VP
Sbjct: 427 WKDVTEDYMKSNKFPNAILSCVINVNRLSDFFYKEEDGYTFPNGEIKRFITSMLKDPVP 485

[Close]

BLAST of MC00g_new0044 vs. ExPASy TrEMBL
Match: A0A6J1CQS0 ((-)-germacrene D synthase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 323 bits (827), Expect = 5.84e-107
Identity = 163/180 (90.56%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AYLVEAKWFH+GHVPSMEEYMEVAIVSAAYYLFASVS LGMGDVASKEAFQWVM
Sbjct: 279 MKRQCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVM 338

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  MLLNA GVIGRLVNDIASHKFEQERGHV  AVECY+KQY VSEEE VGELEKQVIKA
Sbjct: 339 SDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKA 398

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD TEDYMKSTRF NAI+ CVTNVARLSDLFYKEGDGYTFP GETRRFITSILKD VPL
Sbjct: 399 WKDITEDYMKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 458

[Close]

BLAST of MC00g_new0044 vs. ExPASy TrEMBL
Match: A0A6J1CQA8 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013331 PE=4 SV=1)

HSP 1 Score: 323 bits (827), Expect = 1.08e-105
Identity = 163/180 (90.56%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AYLVEAKWFH+GHVPSMEEYMEVAIVSAAYYLFASVS LGMGDVASKEAFQWVM
Sbjct: 379 MKRQCRAYLVEAKWFHKGHVPSMEEYMEVAIVSAAYYLFASVSFLGMGDVASKEAFQWVM 438

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  MLLNA GVIGRLVNDIASHKFEQERGHV  AVECY+KQY VSEEE VGELEKQVIKA
Sbjct: 439 SDPMLLNATGVIGRLVNDIASHKFEQERGHVASAVECYMKQYEVSEEEAVGELEKQVIKA 498

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD TEDYMKSTRF NAI+ CVTNVARLSDLFYKEGDGYTFP GETRRFITSILKD VPL
Sbjct: 499 WKDITEDYMKSTRFPNAIVACVTNVARLSDLFYKEGDGYTFPDGETRRFITSILKDPVPL 558

[Close]

BLAST of MC00g_new0044 vs. ExPASy TrEMBL
Match: A0A6J1CP21 (valencene synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013401 PE=4 SV=1)

HSP 1 Score: 276 bits (705), Expect = 2.85e-88
Identity = 136/180 (75.56%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AY  EAKWF+EG+VP+MEEYMEVA +SA+Y+LFA+ S LGMGDVAS E FQW  
Sbjct: 301 MKRQCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAK 360

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  +LL+A GVIGRL+NDI SHKFEQERGHV  AVECY+KQYGVSE E + ELEKQVIKA
Sbjct: 361 SDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKA 420

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD  EDYMK TRF NAIL CV NV RLSD FYKE DGYTFP+GET RFITS+LKD VPL
Sbjct: 421 WKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 480

[Close]

BLAST of MC00g_new0044 vs. ExPASy TrEMBL
Match: A0A6J1CPK3 ((-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013401 PE=4 SV=1)

HSP 1 Score: 276 bits (705), Expect = 1.99e-87
Identity = 136/180 (75.56%), Postives = 151/180 (83.89%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+AY  EAKWF+EG+VP+MEEYMEVA +SA+Y+LFA+ S LGMGDVAS E FQW  
Sbjct: 374 MKRQCRAYFTEAKWFYEGYVPTMEEYMEVASISASYHLFAAASFLGMGDVASNEVFQWAK 433

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  +LL+A GVIGRL+NDI SHKFEQERGHV  AVECY+KQYGVSE E + ELEKQVIKA
Sbjct: 434 SDPILLSATGVIGRLMNDIVSHKFEQERGHVASAVECYMKQYGVSEGEAIHELEKQVIKA 493

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 179
           WKD  EDYMK TRF NAIL CV NV RLSD FYKE DGYTFP+GET RFITS+LKD VPL
Sbjct: 494 WKDIIEDYMKCTRFPNAILSCVMNVDRLSDFFYKEEDGYTFPNGETNRFITSMLKDPVPL 553

[Close]

BLAST of MC00g_new0044 vs. ExPASy TrEMBL
Match: A0A6J1CR35 ((-)-germacrene D synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013402 PE=4 SV=1)

HSP 1 Score: 265 bits (677), Expect = 5.46e-84
Identity = 129/179 (72.07%), Postives = 150/179 (83.80%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           MKRQC+ YL EAKWF+EG+VP+MEEYME+A +SA+Y+LFA+ S LGMGDV SKE  +W  
Sbjct: 307 MKRQCRVYLAEAKWFYEGYVPTMEEYMEIATISASYHLFAASSFLGMGDVVSKEVLEWAK 366

Query: 61  SH-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           S  ++L+A+G IGRL+NDI SH+FEQERG V  AVECY+KQYGVSEEE V ELEKQVIKA
Sbjct: 367 SDPIMLSASGDIGRLMNDIVSHEFEQERGDVASAVECYMKQYGVSEEEAVVELEKQVIKA 426

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVP 178
           WKD TEDYMKS +F NAIL CV NV RLSD FYKE DGYTFP+GE +RFITS+LKD VP
Sbjct: 427 WKDVTEDYMKSNKFPNAILSCVINVNRLSDFFYKEEDGYTFPNGEIKRFITSMLKDPVP 485

[Close]

BLAST of MC00g_new0044 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.3e-32
Identity = 71/176 (40.34%), Postives = 106/176 (60.23%), Query Frame = 0


Query: 6   KAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVMSH-ML 65
           KAY    +W  +GHVP+ +EYM+V +V+A    +A    +GM D+  KEAF+W+ S+ +L
Sbjct: 426 KAYKQLTEWARKGHVPTFDEYMKVGLVTAGMGDYAGYCFIGMEDINEKEAFEWLNSNPLL 485

Query: 66  LNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKAWKDKT 125
           ++A  V+ R+ ND+ +++ E  RG V   + CY+KQYGV++EE   EL K  I   K   
Sbjct: 486 IDALNVLFRIANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASRELRKMYIYNKKVVV 545

Query: 126 EDYMKS-TRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 180
           E++M S  R    +L    N ARL D+ Y EGDGY+ P G+   F+TS+    +PL
Sbjct: 546 EEFMNSHDRVPRQVLLRCLNFARLFDVIYTEGDGYSEPKGKIEHFMTSLYVHPIPL 601

[Close]

BLAST of MC00g_new0044 vs. TAIR 10
Match: AT1G31950.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 5.8e-31
Identity = 68/177 (38.42%), Postives = 106/177 (59.89%), Query Frame = 0


Query: 6   KAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVMSH-ML 65
           KAY   AKW   GHV + +EYM+V +++A    +A+   +GM D+  KEAF+W+ S+ ++
Sbjct: 430 KAYQEIAKWARTGHVSTFDEYMKVGVLTAGMADYAAYCFIGMEDINEKEAFEWLNSNPLI 489

Query: 66  LNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKAWKDKT 125
           +     + RL ND+ +++ E  RG V   + CY+KQYGV++EE   EL K  +   K   
Sbjct: 490 IKHLTAMFRLANDVGTYETEINRGEVANGLNCYMKQYGVTKEEASRELRKMYVYRKKVVV 549

Query: 126 EDYMKSTRFA--NAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 180
           E++M S        +L C+ N+AR+ D+FY EGDGY+ P G+   F+TS+    +PL
Sbjct: 550 EEFMNSHDHVPRQVLLRCL-NIARIFDVFYTEGDGYSEPKGKIEHFMTSLYLHPIPL 605

[Close]

BLAST of MC00g_new0044 vs. TAIR 10
Match: AT5G23960.1 (terpene synthase 21 )

HSP 1 Score: 131.0 bits (328), Expect = 9.8e-31
Identity = 63/180 (35.00%), Postives = 110/180 (61.11%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           +++    Y+ EAKW  + ++ + +EY E AI+S+ YY   +++ + M DVA  +AF+W+ 
Sbjct: 369 LQKTANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLS 428

Query: 61  SHMLLN-ANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           SH  +  A+ +I R  +DI+S++FE +R HV   ++CY++Q+GVS+E  V  +   V  A
Sbjct: 429 SHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDA 488

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 180
           WKD  ++ M+   F   +L  V N++R+ D+FY+  D YT P    +  I S+L + +P+
Sbjct: 489 WKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPK-LLKEHIVSLLIETIPI 547

[Close]

BLAST of MC00g_new0044 vs. TAIR 10
Match: AT5G23960.2 (terpene synthase 21 )

HSP 1 Score: 131.0 bits (328), Expect = 9.8e-31
Identity = 63/180 (35.00%), Postives = 110/180 (61.11%), Query Frame = 0


Query: 1   MKRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVM 60
           +++    Y+ EAKW  + ++ + +EY E AI+S+ YY   +++ + M DVA  +AF+W+ 
Sbjct: 367 LQKTANGYMQEAKWLKKDYIATFDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLS 426

Query: 61  SHMLLN-ANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKA 120
           SH  +  A+ +I R  +DI+S++FE +R HV   ++CY++Q+GVS+E  V  +   V  A
Sbjct: 427 SHPKIRVASEIISRFTDDISSYEFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDA 486

Query: 121 WKDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRVPL 180
           WKD  ++ M+   F   +L  V N++R+ D+FY+  D YT P    +  I S+L + +P+
Sbjct: 487 WKDLNQELMRPHVFPFPLLMRVLNLSRVIDVFYRYQDAYTNPK-LLKEHIVSLLIETIPI 545

[Close]

BLAST of MC00g_new0044 vs. TAIR 10
Match: AT4G20200.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 129.8 bits (325), Expect = 2.2e-30
Identity = 65/177 (36.72%), Postives = 104/177 (58.76%), Query Frame = 0


Query: 2   KRQCKAYLVEAKWFHEGHVPSMEEYMEVAIVSAAYYLFASVSILGMGDVASKEAFQWVMS 61
           K   KA +   KW    H+PS EEYMEV  V    Y   +   + MG +A+KEAF+W+ S
Sbjct: 426 KAAAKANIDLEKWAQADHIPSFEEYMEVGEVEVTVYASLAGIFMSMGKMATKEAFEWLKS 485

Query: 62  H-MLLNANGVIGRLVNDIASHKFEQERGHVTCAVECYLKQYGVSEEEVVGELEKQVIKAW 121
              L+    + GRL+ND+  ++ +  RG+VT AV CY+KQYGV++EE   EL K V+ A 
Sbjct: 486 RPKLVQYLSIKGRLMNDLMGYEDDMSRGYVTNAVNCYMKQYGVTKEEAFRELYKIVVAAN 545

Query: 122 KDKTEDYMKSTRFANAILPCVTNVARLSDLFYKEGDGYTFPHGETRRFITSILKDRV 178
           K   E+++ +T   + +L    ++AR+  + Y   +G+T P G+ + ++TS+  D++
Sbjct: 546 KTLNEEFLSTTGVPHFLLKATIDLARMMTVAYNVNEGFTNPQGKIKEYMTSMFVDQI 602

The following BLAST results are available for this feature:

BLAST of MC00g_new0044 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6Q3H2	3.4e-44	49.44	Valencene synthase OS=Vitis vinifera OX=29760 GN=ValCS PE=1 SV=1	[more]
F6M8H7	3.8e-43	46.63	Probable sesquiterpene synthase OS=Santalum murrayanum OX=453085 GN=STPS PE=3 SV...	[more]
F6M8H5	6.4e-43	46.59	Probable sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 GN=Sesqu...	[more]
B5A435	8.4e-43	47.16	Sesquiterpene synthase OS=Santalum album OX=35974 PE=1 SV=1	[more]
Q6Q3H3	2.1e-41	46.11	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]

BLAST of MC00g_new0044 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143458.1	1.21e-106	90.56	(-)-germacrene D synthase-like isoform X3 [Momordica charantia]	[more]
XP_022143456.1	2.23e-105	90.56	(-)-germacrene D synthase-like isoform X1 [Momordica charantia]	[more]
XP_022143530.1	5.89e-88	75.56	valencene synthase-like isoform X2 [Momordica charantia]	[more]
XP_022143529.1	4.10e-87	75.56	(-)-germacrene D synthase-like isoform X1 [Momordica charantia]	[more]
XP_022143532.1	1.13e-83	72.07	(-)-germacrene D synthase-like isoform X2 [Momordica charantia]	[more]

BLAST of MC00g_new0044 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQS0	5.84e-107	90.56	(-)-germacrene D synthase-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CQA8	1.08e-105	90.56	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CP21	2.85e-88	75.56	valencene synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11101340...	[more]
A0A6J1CPK3	1.99e-87	75.56	(-)-germacrene D synthase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1CR35	5.46e-84	72.07	(-)-germacrene D synthase-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of MC00g_new0044 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14540.1	2.3e-32	40.34	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT1G31950.1	5.8e-31	38.42	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT5G23960.1	9.8e-31	35.00	terpene synthase 21	[more]
AT5G23960.2	9.8e-31	35.00	terpene synthase 21	[more]
AT4G20200.1	2.2e-30	36.72	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 1..177 e-value: 3.9E-59 score: 202.2
IPR008949	Isoprenoid synthase domain superfamily	SUPERFAMILY	48576	Terpenoid synthases	coord: 1..175
None	No IPR available	PFAM	PF19086	Terpene_syn_C_2	coord: 1..122 e-value: 1.1E-39 score: 136.3
None	No IPR available	PANTHER	PTHR31225:SF111	TERPENE SYNTHASE 2-RELATED	coord: 2..164
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 2..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g_new0044.1	MC00g_new0044.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0010333	terpene synthase activity