MC00g0514 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC00g0514
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
DescriptionPyruvate decarboxylase
Locationscaffold196: 567679 .. 570965 (-)
RNA-Seq ExpressionMC00g0514
SyntenyMC00g0514
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGATCAACCTCGAAAACGGCTTCCAAACAGAAAACCCAGTGGCGCCAGCTCGCTCCAAGCTCAAGCCGTCCACTAATGGCGTATTGAAACCACTAATTTGCATCCAATCCCAACTCGCCGGAGCCCCAAATATTGCAGCGGCCGCCGCCACGCCGTCGACGCTGGGCCACTACCTGGCCCGCCGGCTGGTCGAGATCGGCATCGGCGACATCTTCTCCGTTCCGGGAGATTCCAATCTTGTGCTGCTCGATTACTTCGTTGCGGAAAAGGGGCTGAATCTTGTTGGGTGCTGCAGCGAGCTCAACGCTGGGTACGCGGCGGACGGCTACGCCAGGCGCCGCGGCGTGGGAGCCTGCGCTGTGACGTTCACGGTGGGCGGGCTGAGCCTGATCAACGCCGTGGCCGGAGCTTTCTCGGAGGATCTTCCGGTGGTGTGCATTGTGGGTGGGCCCAATTCCAATGATTATGGGAGTAAAAAGATTCTCCACCATACCATTGGGCTGCCTGATTTCTCCCAAGAACTTCGTTGCTTTCAAAATGTTACTTGTTATCAGGTAATTTCAATTTGTGTTCTATTCTATTCACTACCTCAATTTTCAGATTTGTTTTTCTTAGCTAATTTGTAGTTTCAAAACATGCCCACGTCAACATTTTGATACACAAATACTCAAAGCTAATCGAAATGGATATTTTTTAAAAGTTGCAAGGATGATAGAACCTTTGAAAGTTAGAAAAACTAAAATGAGATTCAAAATTTCAACTTCTTTGTAAATATATGTTTCAATTTTAGAAACTTTAAGACTCGTCTGGTAAAGTTGTTGTTTTCTATTTCTCGTTTTCAATTTTCAAATTTAAACATTTAGCAGTTAACTATGTTTCTTATTTTTCATTTTTCAGAATGTTCTTAAAATTATACAGAAATTTTGAAAATAACGAGACAAGTTTCTTCTCTCTCGTTCTAATTTTTCATTTACTCCAAAACATAAATAAAAAAATTATTTGATCATACTTGAATTCATATATGTTGGATCATGAAATTCATTTTTTAAACAAAAGCAAGAAATTGTAGTGTTATTAAACGGACCCTTATTTACTTTCTTATGTAGGCTGTAATTGACAGTCTGAAAGACGCACAATGGAAGATAGACACAGCAATTTGCAAGTGTTTGGAAGAGAGCAAGCCAGTTTACATTAGCGTTTGCTGCAATTTGGTGGCCACTCCTCATCCTTCTTTCTCGATCCAATCCCTCATCCCACTTTCCCTCCCTCCCAAGTAATATCATTCGATCCCATTTTACTCCTCACTTCAAAATGTCAGTGCTTCAATTTCATGAAAATATAGATGGAAATATTCATATAATGTCAATATTCATATATAATTCTATAAACATAGGAGAAATTAAAAAATCGTACAAAAAATATTTAAATTAACAAATAAACATATTTTTATTTCTAAATAGGTGAGAAATGATTAATATTAATATTATGTATATTAATAGATACATTATTGATTTTTTTAATACGATAGAAATGTCAATTACCGAACCCGCGAATTTATGGAAATATTGATAGATATGTCCCTGTGTTTGCCGATATTTAATATGCCCTTTAACTCACAAAATTTTCAAAAAAAAGAAAAAAAAAAAGAGTGTTTTCTATGGTTAATTTTCTGCCTGAATTATTTGATGATTCCTCAGGCAAAGTAACCAGATGGGTCTGGAGGCGGCGGTGGAGAAGGCAGCTCAGCTTCTAAATAGCGCCGTGAAACCAGTGGCCGTCGCCGGAAAGAAGCTGCGCCTCGCCAAAGCCCAGGCGGCGTTCGTGGAGCTGGCCGACGCTTGTGGCTACGCCGTCGCCGTCACGCCGTCGGCGAAAGGCCTGTTCCCGGAGAACCACCCTCATTTCATCGGCACTTACTGGGGAGTTCTCAGCACCGCCTTCTGCGGTGAGACCGTTGAGATCGCCGACGCCTCGCTCTTCGCCGGGCCCACTTTCGACGAACTTGAAACCGTCGGATTCTCTCTTTGCTACAAAAAGAACAACGCGATTATCGTGGAGCCGGAGCGCGTCGTGTTTCCTAATGGGCCGAGTTTTGGGCCCATTTTAATGATGGACTTCCTTCGGGCCCTAACTAAACGCCTCAAGCCCAATCCAACGGCCTACGAGAATTACCGCCGGATTTATGTTCCGGAGTCGGGTCCACTCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAATGTTCTTTTCAAACATATTCAGAAAATGTTGTCGAGTAACATGGCTGTGATTTCTGAGGCCGGTGACTCTTGGTTTCACTGTCAAAAATTGAAACTACCACAATCGTGCGGGTAAAATTCTTATCAAATTTAACACAGTGATTAAAGTATTGTGAGTTGAAGATATATGAATGACAGTTTGAAGAAATGTAGGTATGAGATGCAGATAATCTATGCATCCATTGGATGGTCTTTAGGGGCAACTCTCGGATATGCACAAGCTGCATCAAACGAACGAATGGTTCTCTGTATGGGTGATGGGAGCTTTCAGGTCGAGCTTATTTCAATATGTCATTCTTATTATATTTTGATCTTTAAATTTTCAATAGAGAAAATAACTTTATGTGCAGATGGCTCCACAAGATTTATCGACGATGTTAAGGTTGGGACAAAGGAACATAATATTTTTGATAAATAATGGTGGATACACCATTGAAGTAGAAATTCACGATGGACCTTACAATGTCATCAAGAATTGGGACTATACCGCCCTCGTTGATGCCATGCATAATGCTAATGGTAATTGTTGGACCACCAAGGTAAAATCTCAATATCATTTTTCCTTTTTTATTTTCATATACGCCTTACAAAATAAGGAAAACAAATATCTTTCTTTTTAAAAAAAATATGTTTTCATTAAGTAAGAAAATTATCTTAAAAGTTCTTCCAAAACAAGCATAACTAGTTTTCTCGATTAAATCAGAGTATCGAGGTGCATAAGTTAGTTGAGAGCCATGAAAATTGTATAATCAAAATGTTGCATTGCTGATTTTTACGTTGCGTTGCTGATTTTTTTTTCTCTCTAGTTTGTTTCATGGGTTCTAAATTTCAACTTGAAGGTTAAAACAGAAGAAGAATTGGTGAAAGCAATCCAAATAGCAATGGGAGAAAAAAATGATTGCTTGTGTTTCATAGAGACTATTGTCCATCGAGATGATACAAGTAAAGAGTTGCTTGAATTTGGGAACAAGCTTGCTGCCATTAATAGTCGTCCTCCTAAACCTTTA

mRNA sequence

ATGCCGATCAACCTCGAAAACGGCTTCCAAACAGAAAACCCAGTGGCGCCAGCTCGCTCCAAGCTCAAGCCGTCCACTAATGGCGTATTGAAACCACTAATTTGCATCCAATCCCAACTCGCCGGAGCCCCAAATATTGCAGCGGCCGCCGCCACGCCGTCGACGCTGGGCCACTACCTGGCCCGCCGGCTGGTCGAGATCGGCATCGGCGACATCTTCTCCGTTCCGGGAGATTCCAATCTTGTGCTGCTCGATTACTTCGTTGCGGAAAAGGGGCTGAATCTTGTTGGGTGCTGCAGCGAGCTCAACGCTGGGTACGCGGCGGACGGCTACGCCAGGCGCCGCGGCGTGGGAGCCTGCGCTGTGACGTTCACGGTGGGCGGGCTGAGCCTGATCAACGCCGTGGCCGGAGCTTTCTCGGAGGATCTTCCGGTGGTGTGCATTGTGGGTGGGCCCAATTCCAATGATTATGGGAGTAAAAAGATTCTCCACCATACCATTGGGCTGCCTGATTTCTCCCAAGAACTTCGTTGCTTTCAAAATGTTACTTGTTATCAGGCTGTAATTGACAGTCTGAAAGACGCACAATGGAAGATAGACACAGCAATTTGCAAGTGTTTGGAAGAGAGCAAGCCAGTTTACATTAGCGTTTGCTGCAATTTGGTGGCCACTCCTCATCCTTCTTTCTCGATCCAATCCCTCATCCCACTTTCCCTCCCTCCCAAGCAAAGTAACCAGATGGGTCTGGAGGCGGCGGTGGAGAAGGCAGCTCAGCTTCTAAATAGCGCCGTGAAACCAGTGGCCGTCGCCGGAAAGAAGCTGCGCCTCGCCAAAGCCCAGGCGGCGTTCGTGGAGCTGGCCGACGCTTGTGGCTACGCCGTCGCCGTCACGCCGTCGGCGAAAGGCCTGTTCCCGGAGAACCACCCTCATTTCATCGGCACTTACTGGGGAGTTCTCAGCACCGCCTTCTGCGGTGAGACCGTTGAGATCGCCGACGCCTCGCTCTTCGCCGGGCCCACTTTCGACGAACTTGAAACCGTCGGATTCTCTCTTTGCTACAAAAAGAACAACGCGATTATCGTGGAGCCGGAGCGCGTCGTGTTTCCTAATGGGCCGAGTTTTGGGCCCATTTTAATGATGGACTTCCTTCGGGCCCTAACTAAACGCCTCAAGCCCAATCCAACGGCCTACGAGAATTACCGCCGGATTTATGTTCCGGAGTCGGGTCCACTCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAATGTTCTTTTCAAACATATTCAGAAAATGTTGTCGAGTAACATGGCTGTGATTTCTGAGGCCGGTGACTCTTGGTTTCACTGTCAAAAATTGAAACTACCACAATCGTGCGGGTATGAGATGCAGATAATCTATGCATCCATTGGATGGTCTTTAGGGGCAACTCTCGGATATGCACAAGCTGCATCAAACGAACGAATGGTTCTCTGTATGGGTGATGGGAGCTTTCAGATGGCTCCACAAGATTTATCGACGATGTTAAGGTTGGGACAAAGGAACATAATATTTTTGATAAATAATGGTGGATACACCATTGAAGTAGAAATTCACGATGGACCTTACAATGTCATCAAGAATTGGGACTATACCGCCCTCGTTGATGCCATGCATAATGCTAATGGTAATTGTTGGACCACCAAGGTTAAAACAGAAGAAGAATTGGTGAAAGCAATCCAAATAGCAATGGGAGAAAAAAATGATTGCTTGTGTTTCATAGAGACTATTGTCCATCGAGATGATACAAGTAAAGAGTTGCTTGAATTTGGGAACAAGCTTGCTGCCATTAATAGTCGTCCTCCTAAACCTTTA

Coding sequence (CDS)

ATGCCGATCAACCTCGAAAACGGCTTCCAAACAGAAAACCCAGTGGCGCCAGCTCGCTCCAAGCTCAAGCCGTCCACTAATGGCGTATTGAAACCACTAATTTGCATCCAATCCCAACTCGCCGGAGCCCCAAATATTGCAGCGGCCGCCGCCACGCCGTCGACGCTGGGCCACTACCTGGCCCGCCGGCTGGTCGAGATCGGCATCGGCGACATCTTCTCCGTTCCGGGAGATTCCAATCTTGTGCTGCTCGATTACTTCGTTGCGGAAAAGGGGCTGAATCTTGTTGGGTGCTGCAGCGAGCTCAACGCTGGGTACGCGGCGGACGGCTACGCCAGGCGCCGCGGCGTGGGAGCCTGCGCTGTGACGTTCACGGTGGGCGGGCTGAGCCTGATCAACGCCGTGGCCGGAGCTTTCTCGGAGGATCTTCCGGTGGTGTGCATTGTGGGTGGGCCCAATTCCAATGATTATGGGAGTAAAAAGATTCTCCACCATACCATTGGGCTGCCTGATTTCTCCCAAGAACTTCGTTGCTTTCAAAATGTTACTTGTTATCAGGCTGTAATTGACAGTCTGAAAGACGCACAATGGAAGATAGACACAGCAATTTGCAAGTGTTTGGAAGAGAGCAAGCCAGTTTACATTAGCGTTTGCTGCAATTTGGTGGCCACTCCTCATCCTTCTTTCTCGATCCAATCCCTCATCCCACTTTCCCTCCCTCCCAAGCAAAGTAACCAGATGGGTCTGGAGGCGGCGGTGGAGAAGGCAGCTCAGCTTCTAAATAGCGCCGTGAAACCAGTGGCCGTCGCCGGAAAGAAGCTGCGCCTCGCCAAAGCCCAGGCGGCGTTCGTGGAGCTGGCCGACGCTTGTGGCTACGCCGTCGCCGTCACGCCGTCGGCGAAAGGCCTGTTCCCGGAGAACCACCCTCATTTCATCGGCACTTACTGGGGAGTTCTCAGCACCGCCTTCTGCGGTGAGACCGTTGAGATCGCCGACGCCTCGCTCTTCGCCGGGCCCACTTTCGACGAACTTGAAACCGTCGGATTCTCTCTTTGCTACAAAAAGAACAACGCGATTATCGTGGAGCCGGAGCGCGTCGTGTTTCCTAATGGGCCGAGTTTTGGGCCCATTTTAATGATGGACTTCCTTCGGGCCCTAACTAAACGCCTCAAGCCCAATCCAACGGCCTACGAGAATTACCGCCGGATTTATGTTCCGGAGTCGGGTCCACTCCAGTCGGAGCCGGGCGAGGCCTTGCGGGTCAATGTTCTTTTCAAACATATTCAGAAAATGTTGTCGAGTAACATGGCTGTGATTTCTGAGGCCGGTGACTCTTGGTTTCACTGTCAAAAATTGAAACTACCACAATCGTGCGGGTATGAGATGCAGATAATCTATGCATCCATTGGATGGTCTTTAGGGGCAACTCTCGGATATGCACAAGCTGCATCAAACGAACGAATGGTTCTCTGTATGGGTGATGGGAGCTTTCAGATGGCTCCACAAGATTTATCGACGATGTTAAGGTTGGGACAAAGGAACATAATATTTTTGATAAATAATGGTGGATACACCATTGAAGTAGAAATTCACGATGGACCTTACAATGTCATCAAGAATTGGGACTATACCGCCCTCGTTGATGCCATGCATAATGCTAATGGTAATTGTTGGACCACCAAGGTTAAAACAGAAGAAGAATTGGTGAAAGCAATCCAAATAGCAATGGGAGAAAAAAATGATTGCTTGTGTTTCATAGAGACTATTGTCCATCGAGATGATACAAGTAAAGAGTTGCTTGAATTTGGGAACAAGCTTGCTGCCATTAATAGTCGTCCTCCTAAACCTTTA

Protein sequence

MPINLENGFQTENPVAPARSKLKPSTNGVLKPLICIQSQLAGAPNIAAAAATPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINSRPPKPL
Homology
BLAST of MC00g0514 vs. ExPASy Swiss-Prot
Match: A2XFI3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=2)

HSP 1 Score: 823.9 bits (2127), Expect = 1.2e-237
Identity = 388/570 (68.07%), Postives = 468/570 (82.11%), Query Frame = 0

Query: 47  AAAAATPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGY 106
           A  +A  ++LG +LARRLV++G+ D+F+VPGD NL LLD+ +AE GL LVGCC+ELNAGY
Sbjct: 37  APVSAGEASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGY 96

Query: 107 AADGYARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHT 166
           AADGYAR RGVGACAVTFTVGGLS++NA+AGA+SE+LPV+CI GGPNSNDYG+ +ILHHT
Sbjct: 97  AADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHT 156

Query: 167 IGLPDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPH 226
           IGLPDFSQELRCFQ VTC+QAV+ +L+DA  +IDTAI   L ESKPVY+S+ CNL   PH
Sbjct: 157 IGLPDFSQELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPH 216

Query: 227 PSFSIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVEL 286
           P+FS +  +P  L P+ SN+MGLEAAVE   + LN AVKPV V G KLR+AKA  AFV+L
Sbjct: 217 PTFS-RDPVPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDL 276

Query: 287 ADACGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELET 346
            DA GYA AV PSAKGL PE HPHFIGTYWG +STAFC E VE ADA LFAGP F++  +
Sbjct: 277 VDASGYAYAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSS 336

Query: 347 VGFSLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVP 406
           VG+S   KK+ AIIV+PERV+  NGP+FG ++M +FL  L KR+  N TAYENY+RI+VP
Sbjct: 337 VGYSFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVP 396

Query: 407 ESGPLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIY 466
           E  PL+SEP E LRVNVLFKH+QKML+S+ AVI+E GDSWF+CQKLKLP+ CGYE Q+ Y
Sbjct: 397 EGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQY 456

Query: 467 ASIGWSLGATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTI 526
            SIGWS+GA LGYAQ A ++R++ C+GDGSFQ+  QD+STM+R  Q +IIFLINNGGYTI
Sbjct: 457 GSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTI 516

Query: 527 EVEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFI 586
           EVEIHDGPYNVIKNW+YT LVDA+HN  G CWT+KVK EEEL +AI +A+GEK DCLCFI
Sbjct: 517 EVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMALGEKKDCLCFI 576

Query: 587 ETIVHRDDTSKELLEFGNKLAAINSRPPKP 617
           E I H+DDTSKELLE+G++++A NSRPP P
Sbjct: 577 EVIAHKDDTSKELLEWGSRVSAANSRPPNP 605

BLAST of MC00g0514 vs. ExPASy Swiss-Prot
Match: A2Y5L9 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 2.9e-236
Identity = 395/600 (65.83%), Postives = 480/600 (80.00%), Query Frame = 0

Query: 17  PARSKLKPSTNGVLKPLICIQSQLAGAPNIAAAAATPSTLGHYLARRLVEIGIGDIFSVP 76
           P+    KPS N V   L  + S     P + +AA   +TLG +LARRLV+IG  D+F+VP
Sbjct: 9   PSNGVAKPSCNSV-GSLPVVSSNAVIHPPVTSAAG--ATLGRHLARRLVQIGATDVFAVP 68

Query: 77  GDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVA 136
           GD NL LLDY +AE GL L+GCC+ELNAGYAADGYAR RGVGACAVTFTVGGLS++NA+A
Sbjct: 69  GDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIA 128

Query: 137 GAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDSLKDAQ 196
           GA+SE+LPV+CIVGGPNSNDYG+ +ILHHTIGLPDFSQELRCFQ +TCYQAVI++L DA 
Sbjct: 129 GAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAVINNLDDAH 188

Query: 197 WKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEAAVEKA 256
            +IDTAI   L ESKPVYISV CNL    HP+FS +  +PL + P+ SN+  LE AVE A
Sbjct: 189 EQIDTAIATALRESKPVYISVGCNLAGLSHPTFS-REPVPLFISPRLSNKANLEYAVEAA 248

Query: 257 AQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYW 316
           A  LN AVKPV V G K+R+AKA+ AF  +A++ GY +AV PSAKGL PE+HP FIGTYW
Sbjct: 249 ADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPIAVMPSAKGLVPEHHPRFIGTYW 308

Query: 317 GVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNGPSFGP 376
           G +ST FC E VE ADA LFAGP F++  +VG+SL  K+  A+IV+P+RVV  NGP+FG 
Sbjct: 309 GAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNGPAFGC 368

Query: 377 ILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNM 436
           ILM +FL AL KRL  N TAY+NYRRI++P+  P   +P E LRVN+LFKHI++MLS + 
Sbjct: 369 ILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDT 428

Query: 437 AVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLCMGDGS 496
           AVI+E GDSWF+CQKL+LP+ CGYE Q+ Y SIGWS+GATLGYAQAA ++R++ C+GDGS
Sbjct: 429 AVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGS 488

Query: 497 FQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNANGN 556
           FQM  QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYT L+DA+HN++GN
Sbjct: 489 FQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIHNSDGN 548

Query: 557 CWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINSRPPKP 616
           CWT KV+TEEEL++AI  A G K DCLCFIE IVH+DDTSKELLE+G++++A NSRPP P
Sbjct: 549 CWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPPNP 604

BLAST of MC00g0514 vs. ExPASy Swiss-Prot
Match: Q10MW3 (Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 3.8e-236
Identity = 388/570 (68.07%), Postives = 468/570 (82.11%), Query Frame = 0

Query: 47  AAAAATPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGY 106
           A  +A  ++LG +LARRLV++G+ D+F+VPGD NL LLD+ +AE GL LVGCC+ELNAGY
Sbjct: 37  APVSAGEASLGRHLARRLVQVGVSDVFAVPGDFNLTLLDHLIAEPGLRLVGCCNELNAGY 96

Query: 107 AADGYARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHT 166
           AADGYAR RGVGACAVTFTVGGLS++NA+AGA+SE+LPV+CI GGPNSNDYG+ +ILHHT
Sbjct: 97  AADGYARARGVGACAVTFTVGGLSVLNAIAGAYSENLPVICIAGGPNSNDYGTNRILHHT 156

Query: 167 IGLPDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPH 226
           IGLPDFSQELRCFQ VTC+QAV+ +L+DA  +IDTAI   L ESKPVY+S+ CNL   PH
Sbjct: 157 IGLPDFSQELRCFQTVTCHQAVVTNLEDAHEQIDTAIATALRESKPVYLSISCNLPGLPH 216

Query: 227 PSFSIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVEL 286
           P+FS +  +P  L P+ SN+MGLEAAVE   + LN AVKPV V G KLR+AKA  AFV+L
Sbjct: 217 PTFS-RDPVPFFLAPRLSNKMGLEAAVEATVEFLNKAVKPVLVGGPKLRVAKAGKAFVDL 276

Query: 287 ADACGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELET 346
            DA GYA AV PSAKGL PE HPHFIGTYWG +STAFC E VE ADA LFAGP F++  +
Sbjct: 277 VDASGYAYAVMPSAKGLVPETHPHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSS 336

Query: 347 VGFSLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVP 406
           VG+S   KK+ AIIV+PERV+  NGP+FG ++M +FL  L KR+  N TAYENY+RI+VP
Sbjct: 337 VGYSFLLKKDKAIIVQPERVIVGNGPAFGCVMMKEFLSELAKRVNKNTTAYENYKRIFVP 396

Query: 407 ESGPLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIY 466
           E  PL+SEP E LRVNVLFKH+QKML+S+ AVI+E GDSWF+CQKLKLP+ CGYE Q+ Y
Sbjct: 397 EGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQY 456

Query: 467 ASIGWSLGATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTI 526
            SIGWS+GA LGYAQ A ++R++ C+GDGSFQ+  QD+STM+R  Q +IIFLINNGGYTI
Sbjct: 457 GSIGWSVGALLGYAQGAKDKRVIACIGDGSFQVTAQDVSTMIRCAQNSIIFLINNGGYTI 516

Query: 527 EVEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFI 586
           EVEIHDGPYNVIKNW+YT LVDA+HN  G CWT+KVK EEEL +AI +A+GEK DCLCFI
Sbjct: 517 EVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTSKVKCEEELTEAIGMALGEK-DCLCFI 576

Query: 587 ETIVHRDDTSKELLEFGNKLAAINSRPPKP 617
           E I H+DDTSKELLE+G++++A NSRPP P
Sbjct: 577 EVIAHKDDTSKELLEWGSRVSAANSRPPNP 604

BLAST of MC00g0514 vs. ExPASy Swiss-Prot
Match: P28516 (Pyruvate decarboxylase 1 OS=Zea mays OX=4577 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 6.5e-236
Identity = 394/602 (65.45%), Postives = 477/602 (79.24%), Query Frame = 0

Query: 17  PARSKLKPSTNGVLKPLICIQSQLAGAPNIAAAAATP--STLGHYLARRLVEIGIGDIFS 76
           PA    KP+ NGV    +     +   P  AAA   P  +TLG +LARRLV+IG  D+F+
Sbjct: 9   PANGVAKPTCNGVGALPVANSHAIIATPAAAAATLAPAGATLGRHLARRLVQIGASDVFA 68

Query: 77  VPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINA 136
           VPGD NL LLDY +AE GL LVGCC+ELNAGYAADGYAR RGVGACAVTFTVGGLS++NA
Sbjct: 69  VPGDFNLTLLDYLIAEPGLTLVGCCNELNAGYAADGYARSRGVGACAVTFTVGGLSVLNA 128

Query: 137 VAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDSLKD 196
           +AGA+SE+LPVVCIVGGPNSNDYG+ +ILHHTIGLPDFSQELRCFQ +TCYQA+I++L D
Sbjct: 129 IAGAYSENLPVVCIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAIINNLDD 188

Query: 197 AQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEAAVE 256
           A  +IDTAI   L ESKPVYISV CNL    HP+FS +  +P+ + P+ SN+  LE AVE
Sbjct: 189 AHEQIDTAIATALRESKPVYISVSCNLAGLSHPTFS-RDPVPMFISPRLSNKANLEYAVE 248

Query: 257 KAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGT 316
            AA  LN AVKPV V G K+R+AKA+ AF  +ADA GY  AV P+AKGL PE+HP FIGT
Sbjct: 249 AAADFLNKAVKPVMVGGPKIRVAKAREAFAAVADASGYPFAVMPAAKGLVPEHHPRFIGT 308

Query: 317 YWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNGPSF 376
           YWG +ST FC E VE ADA LFAGP F++  +VG+SL  K+  A+IV+P+R+V  +GP+F
Sbjct: 309 YWGAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRMVVGDGPAF 368

Query: 377 GPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSS 436
           G ILM +FLRAL KRL+ N TAY+NYRRI+VP+  P   +P E LRVNVLFKHI+ MLS 
Sbjct: 369 GCILMPEFLRALAKRLRRNTTAYDNYRRIFVPDREPPNGKPNEPLRVNVLFKHIKGMLSG 428

Query: 437 NMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLCMGD 496
           + AV++E GDSWF+CQKL+LP+ CGYE Q+ Y SIGWS+GATLGYAQAA ++R++ C+GD
Sbjct: 429 DSAVVAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVIACIGD 488

Query: 497 GSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNAN 556
           GSFQ+  QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYT LV+A+HN+ 
Sbjct: 489 GSFQVTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVNAIHNSE 548

Query: 557 GNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINSRPP 616
           GNCWT KV+TEE+L +AI    G K DCLCFIE IVH+DDTSKELLE+G++++A NSRPP
Sbjct: 549 GNCWTMKVRTEEQLKEAIATVTGAKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPP 608

BLAST of MC00g0514 vs. ExPASy Swiss-Prot
Match: Q0DHF6 (Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 6.5e-236
Identity = 394/600 (65.67%), Postives = 478/600 (79.67%), Query Frame = 0

Query: 17  PARSKLKPSTNGVLKPLICIQSQLAGAPNIAAAAATPSTLGHYLARRLVEIGIGDIFSVP 76
           P+    KPS N V    +   + +   P  +AA A   TLG +LARRLV+IG  D+F+VP
Sbjct: 9   PSNGVAKPSCNSVGSLPVVSSNAVINPPVTSAAGA---TLGRHLARRLVQIGATDVFAVP 68

Query: 77  GDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVA 136
           GD NL LLDY +AE GL L+GCC+ELNAGYAADGYAR RGVGACAVTFTVGGLS++NA+A
Sbjct: 69  GDFNLTLLDYLIAEPGLKLIGCCNELNAGYAADGYARARGVGACAVTFTVGGLSVLNAIA 128

Query: 137 GAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDSLKDAQ 196
           GA+SE+LPV+CIVGGPNSNDYG+ +ILHHTIGLPDFSQELRCFQ +TCYQAVI++L DA 
Sbjct: 129 GAYSENLPVICIVGGPNSNDYGTNRILHHTIGLPDFSQELRCFQTITCYQAVINNLDDAH 188

Query: 197 WKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEAAVEKA 256
            +IDTAI   L ESKPVYISV CNL    HP+FS +  +PL + P+ SN+  LE AVE A
Sbjct: 189 EQIDTAIATALRESKPVYISVGCNLAGLSHPTFS-REPVPLFISPRLSNKANLEYAVEAA 248

Query: 257 AQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYW 316
           A  LN AVKPV V G K+R+AKA+ AF  +A++ GY  AV PSAKGL PE+HP FIGTYW
Sbjct: 249 ADFLNKAVKPVMVGGPKIRVAKAKKAFAGIAESSGYPFAVMPSAKGLVPEHHPRFIGTYW 308

Query: 317 GVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNGPSFGP 376
           G +ST FC E VE ADA LFAGP F++  +VG+SL  K+  A+IV+P+RVV  NGP+FG 
Sbjct: 309 GAVSTTFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKREKAVIVQPDRVVVGNGPAFGC 368

Query: 377 ILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNM 436
           ILM +FL AL KRL  N TAY+NYRRI++P+  P   +P E LRVN+LFKHI++MLS + 
Sbjct: 369 ILMTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDT 428

Query: 437 AVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLCMGDGS 496
           AVI+E GDSWF+CQKL+LP+ CGYE Q+ Y SIGWS+GATLGYAQAA ++R++ C+GDGS
Sbjct: 429 AVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDKRVISCIGDGS 488

Query: 497 FQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNANGN 556
           FQM  QD+STMLR GQ++IIFLINNGGYTIEVEIHDGPYNVIKNWDYT L+DA+HN++GN
Sbjct: 489 FQMTAQDVSTMLRCGQKSIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLIDAIHNSDGN 548

Query: 557 CWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINSRPPKP 616
           CWT KV+TEEEL++AI  A G K DCLCFIE IVH+DDTSKELLE+G++++A NSRPP P
Sbjct: 549 CWTKKVRTEEELIEAIATATGAKKDCLCFIEIIVHKDDTSKELLEWGSRVSAANSRPPNP 604

BLAST of MC00g0514 vs. NCBI nr
Match: XP_022144001.1 (pyruvate decarboxylase 1-like [Momordica charantia])

HSP 1 Score: 1041 bits (2692), Expect = 0.0
Identity = 512/620 (82.58%), Postives = 553/620 (89.19%), Query Frame = 0

Query: 1   MPINLENGFQTENPVAPARSKLKPSTNGVLKPL---ICIQSQLAGAPNIAAAAATPSTLG 60
           +P  LENGF TE P        KP TNG   P    I IQ+Q          +  P TLG
Sbjct: 3   LPTKLENGFPTETPTP------KPLTNGAAAPTNKPIQIQTQ----------SPPPWTLG 62

Query: 61  HYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGV 120
           HYLARRLVEIG+GDIFSVPGD NL+LLDYFVAE GLNLVGCCSELNAGYAADGYARRRGV
Sbjct: 63  HYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGLNLVGCCSELNAGYAADGYARRRGV 122

Query: 121 GACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELR 180
           GACAVTFTVGGLSLINAVAGA+SEDLPV+CIVGGPNSNDYGSKKI+HHTIGLPDFSQELR
Sbjct: 123 GACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQELR 182

Query: 181 CFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPL 240
           CFQNVTCYQA+ID++++ QW+ID AICKCL E KPVYIS+CCNLVA PHPSFS++ LIPL
Sbjct: 183 CFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPVYISICCNLVAIPHPSFSMERLIPL 242

Query: 241 SLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVT 300
           SL PKQSNQMGLE A EKAAQLLNSAVKPV VAGKKLR AKAQAAFVELADACGYAVAVT
Sbjct: 243 SLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRPAKAQAAFVELADACGYAVAVT 302

Query: 301 PSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNN 360
           PSAKGLFPENHPHFIGTYWG +STAFCGETVEIADAS+FAG T DELETV FSL YKKN 
Sbjct: 303 PSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYKKNK 362

Query: 361 AIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGE 420
           AIIVEPERVVFPNGPSFGPILM DFLRAL +RLKPNPTAYENYRRIYVPESGPLQSEPGE
Sbjct: 363 AIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSEPGE 422

Query: 421 ALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATL 480
           ALRVNVLFKHIQKMLSSNM VISEAGDSWFHCQKLKLP+SCGYE+Q++YASIGWSLGATL
Sbjct: 423 ALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLGATL 482

Query: 481 GYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNV 540
           GYAQAA  +R+VLC+GDGSFQM+PQD+STMLRLGQ+NIIFLINNGGYTIEVEIHDGPYNV
Sbjct: 483 GYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGPYNV 542

Query: 541 IKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSK 600
           IKNWDYTA+V+AMHN +G CWT KV TEEELVKAI+ A  ++NDCLCFIE I+HRDDTSK
Sbjct: 543 IKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDDTSK 602

Query: 601 ELLEFGNKLAAINSRPPKPL 617
           ELLEFG+K+AAI+ RPP+PL
Sbjct: 603 ELLEFGSKIAAISCRPPRPL 606

BLAST of MC00g0514 vs. NCBI nr
Match: XP_023544035.1 (pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1034 bits (2674), Expect = 0.0
Identity = 509/624 (81.57%), Postives = 555/624 (88.94%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKPST-NGVLKPLICIQSQLAG-APNIAAAAA 60
           MPINLENGFQ E        P+A  RS LKP T NG  KP   +Q+        I  +  
Sbjct: 1   MPINLENGFQAETFSSTIPMPMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
           TPSTLGHYLARRLV+IG+ DIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY
Sbjct: 61  TPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPD 180
           ARRRGVGAC VTFTVGGLSLINA+AGA+SEDLP+VCIVG PNSN+YGS KILHHTIGLPD
Sbjct: 121 ARRRGVGACVVTFTVGGLSLINAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPD 180

Query: 181 FSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSI 240
           FSQELRCFQNVTCYQAVI++L+DAQW+IDTAI KCLE+SKPVYIS+ CNL A PHPSFS+
Sbjct: 181 FSQELRCFQNVTCYQAVINNLEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSM 240

Query: 241 QSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACG 300
           +  +PLS+  KQ+NQMGLE AVE AAQLLN+AVKPV V GKKLR+AKAQAAFVELAD C 
Sbjct: 241 KQFLPLSISSKQTNQMGLEMAVEMAAQLLNTAVKPVMVGGKKLRVAKAQAAFVELADVCD 300

Query: 301 YAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSL 360
           YAVAVTPSAKG+FPENH HFIGTYWG LS+AFCGETVEIADAS+FAG  FDELETVG+SL
Sbjct: 301 YAVAVTPSAKGMFPENHRHFIGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSL 360

Query: 361 CYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPL 420
            YKKN AIIVE +RVVFPNGPSFGPILM DFLRAL KRLKPNPTAYENY RIY+P+SGP 
Sbjct: 361 SYKKNKAIIVESDRVVFPNGPSFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPT 420

Query: 421 QSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGW 480
           QSEPGEALRVNVLFKHIQKMLS NMAVISEAGDSWFHCQKL+LP+SCG+EMQ++YASIGW
Sbjct: 421 QSEPGEALRVNVLFKHIQKMLSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGW 480

Query: 481 SLGATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIH 540
           SLGATLGYAQA+  +R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIH
Sbjct: 481 SLGATLGYAQASPEDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIH 540

Query: 541 DGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVH 600
           DGPYNVIKNWDYT LV+AMHN  GNCWTTKV+TEEELVKAI++A G+KN+CLCFIE IVH
Sbjct: 541 DGPYNVIKNWDYTGLVEAMHNQTGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVH 600

Query: 601 RDDTSKELLEFGNKLAAINSRPPK 615
           RDDTSKELL+FGNKLAA NSRPPK
Sbjct: 601 RDDTSKELLDFGNKLAATNSRPPK 624

BLAST of MC00g0514 vs. NCBI nr
Match: XP_008464266.1 (PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037738.1 pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 515/630 (81.75%), Postives = 556/630 (88.25%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKP-STNGVLKPLICIQSQLAG-APNIAAAAA 60
           MPINLENG Q E        P    RS LKP +TNGV KP    Q+      P +A  + 
Sbjct: 1   MPINLENGLQAETISSTMPMPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
            PSTLGHYLARRLV+IGI DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGY
Sbjct: 61  VPSTLGHYLARRLVQIGISDIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYG--SKKILHHTIGL 180
           ARRRGVGACAVTFTVGGLSLINA+AGA+SEDLPVVCIVGGPNSN+YG  +KKILHHTIGL
Sbjct: 121 ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGL 180

Query: 181 PDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSF 240
           PDFSQEL+CF+NVTCYQAVI++LKDAQW+ID AI KCLEESKPVYIS+ CNL A PHPSF
Sbjct: 181 PDFSQELQCFRNVTCYQAVINNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSF 240

Query: 241 SIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADA 300
           S+Q LIPLS+ PKQSNQM LE AVEKAAQLLN AVKPV V GKKLR+AKAQAAFVELA+ 
Sbjct: 241 SVQQLIPLSISPKQSNQMSLEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANT 300

Query: 301 CGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGF 360
           C YAVAVTPSAKG+FPENHPHF+GTYWGVLSTAFCGETVEIADAS+F G  FDELETVGF
Sbjct: 301 CDYAVAVTPSAKGMFPENHPHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGF 360

Query: 361 SLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESG 420
           SL YKKN AIIVE + VVFPNGP FGPILM DFL AL KR+KPN TAYENYRRIY+PES 
Sbjct: 361 SLSYKKNKAIIVETDHVVFPNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESS 420

Query: 421 PLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASI 480
           P  SEPGEALRVNVLFKHIQKML+++M VISEAGDSWFH QKL+LPQSCGYEMQI+YASI
Sbjct: 421 PATSEPGEALRVNVLFKHIQKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASI 480

Query: 481 GWSLGATLGYAQAAS--NERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540
           GWSLGATLGYAQA+S  N+R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE
Sbjct: 481 GWSLGATLGYAQASSSSNDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540

Query: 541 VEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIE 600
           VEIHDGPYNVIKNWDY+ALVDAMHN NGNCWTTKV+TEEELVKAI+IA  + NDCLCFIE
Sbjct: 541 VEIHDGPYNVIKNWDYSALVDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIE 600

Query: 601 TIVHRDDTSKELLEFGNKLAAINSRPPKPL 617
            IVHRDDTSKELLEFGNKLA +NSRPP+PL
Sbjct: 601 VIVHRDDTSKELLEFGNKLAEMNSRPPEPL 630

BLAST of MC00g0514 vs. NCBI nr
Match: TYK10923.1 (pyruvate decarboxylase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 515/630 (81.75%), Postives = 556/630 (88.25%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKP-STNGVLKPLICIQSQLAG-APNIAAAAA 60
           MPINLENG Q E        P    RS LKP +TNGV KP    Q+      P +A  + 
Sbjct: 1   MPINLENGLQAETISSTMPMPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
            PSTLGHYLARRLV+IGI DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGY
Sbjct: 61  VPSTLGHYLARRLVQIGISDIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYG--SKKILHHTIGL 180
           ARRRGVGACAVTFTVGGLSLINA+AGA+SEDLPVVCIVGGPNSN+YG  +KKILHHTIGL
Sbjct: 121 ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGL 180

Query: 181 PDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSF 240
           PDFSQEL+CF+NVTCYQAVI++LKDAQW+ID AI KCLEESKPVYIS+ CNL A PHPSF
Sbjct: 181 PDFSQELQCFRNVTCYQAVINNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSF 240

Query: 241 SIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADA 300
           S+Q LIPLS+ PKQSNQM LE AVEKAAQLLN AVKPV V GKKLR+AKAQAAFVELA+ 
Sbjct: 241 SMQQLIPLSISPKQSNQMSLEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANT 300

Query: 301 CGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGF 360
           C YAVAVTPSAKG+FPENHPHF+GTYWGVLSTAFCGETVEIADAS+F G  FDELETVGF
Sbjct: 301 CDYAVAVTPSAKGMFPENHPHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGF 360

Query: 361 SLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESG 420
           SL YKKN AIIVE + VVFPNGP FGPILM DFL AL KR+KPN TAYENYRRIY+PES 
Sbjct: 361 SLSYKKNKAIIVETDHVVFPNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESS 420

Query: 421 PLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASI 480
           P  SEPGEALRVNVLFKHIQKML+++M VISEAGDSWFH QKL+LPQSCGYEMQI+YASI
Sbjct: 421 PATSEPGEALRVNVLFKHIQKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASI 480

Query: 481 GWSLGATLGYAQAAS--NERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540
           GWSLGATLGYAQA+S  N+R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE
Sbjct: 481 GWSLGATLGYAQASSSSNDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540

Query: 541 VEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIE 600
           VEIHDGPYNVIKNWDY+ALVDAMHN NGNCWTTKV+TEEELVKAI+IA  + NDCLCFIE
Sbjct: 541 VEIHDGPYNVIKNWDYSALVDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIE 600

Query: 601 TIVHRDDTSKELLEFGNKLAAINSRPPKPL 617
            IVHRDDTSKELLEFGNKLA +NSRPP+PL
Sbjct: 601 VIVHRDDTSKELLEFGNKLAEMNSRPPEPL 630

BLAST of MC00g0514 vs. NCBI nr
Match: XP_038882338.1 (pyruvate decarboxylase 1-like, partial [Benincasa hispida])

HSP 1 Score: 1019 bits (2635), Expect = 0.0
Identity = 507/624 (81.25%), Postives = 549/624 (87.98%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKPST-NGVLKPLICIQS-QLAGAPNIAAAAA 60
           M INLENGFQ+E        P    RS LKP T NG+ KP    Q+      P +A    
Sbjct: 1   MSINLENGFQSETLSSTIPIPTLATRSMLKPPTPNGIYKPHSKAQTINTNNEPQMAEPTV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
            PSTLGHYLARRLV+IG+ DIFSVPGDSNLVLLDYFVAE GLNLVGCCSELNAGYAADGY
Sbjct: 61  IPSTLGHYLARRLVQIGVSDIFSVPGDSNLVLLDYFVAEIGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGS-KKILHHTIGLP 180
           AR RG+GAC VTFTVGGLSLIN++AGA+SEDLPVVCIVGGPNSN+YGS KKILHHTIGLP
Sbjct: 121 ARHRGIGACVVTFTVGGLSLINSIAGAYSEDLPVVCIVGGPNSNEYGSNKKILHHTIGLP 180

Query: 181 DFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFS 240
           DF+QEL+CF+NVTCYQAVI++L+DAQW+ID AI KCLEESKPVYIS+ CNLVA PHPSFS
Sbjct: 181 DFTQELQCFKNVTCYQAVINNLEDAQWEIDCAISKCLEESKPVYISIACNLVAIPHPSFS 240

Query: 241 IQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADAC 300
            Q LIP+S   KQSNQM LE AVEKAA+LLNSAVKPV V GKKLR AKAQA FVELA+AC
Sbjct: 241 AQQLIPISYSAKQSNQMALEMAVEKAARLLNSAVKPVLVGGKKLRPAKAQAVFVELANAC 300

Query: 301 GYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFS 360
            YAVAVTPSAKG+FPE HPHFIGTYWGVLSTAFCGETVEIADAS+F G  FDELETVGFS
Sbjct: 301 EYAVAVTPSAKGMFPEQHPHFIGTYWGVLSTAFCGETVEIADASIFVGAMFDELETVGFS 360

Query: 361 LCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGP 420
           L YKKNNAII+E + VVFPNGPSFGPILM DFL AL KRLKPN  AYENYRRIY+PES P
Sbjct: 361 LSYKKNNAIIIETDHVVFPNGPSFGPILMKDFLWALAKRLKPNAKAYENYRRIYIPESSP 420

Query: 421 LQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIG 480
            +SEPGEALRVNVLFKHIQKMLSSNM VISEAGDSWFHCQKL+LP+SCGYEMQ++YASIG
Sbjct: 421 AKSEPGEALRVNVLFKHIQKMLSSNMVVISEAGDSWFHCQKLELPRSCGYEMQLVYASIG 480

Query: 481 WSLGATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEI 540
           WSLGATLGYAQA+ ++R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEI
Sbjct: 481 WSLGATLGYAQASLDDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEI 540

Query: 541 HDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIV 600
           HDGPYNVIKNWDYTALVDAMHN NGNCWT KV+TEEELVKAI+IA G++ DCLCFIE IV
Sbjct: 541 HDGPYNVIKNWDYTALVDAMHNHNGNCWTAKVQTEEELVKAIEIAKGDRKDCLCFIEAIV 600

Query: 601 HRDDTSKELLEFGNKLAAINSRPP 614
           H+DDTSKELLEFGNKLAAINSRPP
Sbjct: 601 HKDDTSKELLEFGNKLAAINSRPP 624

BLAST of MC00g0514 vs. ExPASy TrEMBL
Match: A0A6J1CS04 (Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1)

HSP 1 Score: 1041 bits (2692), Expect = 0.0
Identity = 512/620 (82.58%), Postives = 553/620 (89.19%), Query Frame = 0

Query: 1   MPINLENGFQTENPVAPARSKLKPSTNGVLKPL---ICIQSQLAGAPNIAAAAATPSTLG 60
           +P  LENGF TE P        KP TNG   P    I IQ+Q          +  P TLG
Sbjct: 3   LPTKLENGFPTETPTP------KPLTNGAAAPTNKPIQIQTQ----------SPPPWTLG 62

Query: 61  HYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGV 120
           HYLARRLVEIG+GDIFSVPGD NL+LLDYFVAE GLNLVGCCSELNAGYAADGYARRRGV
Sbjct: 63  HYLARRLVEIGVGDIFSVPGDXNLLLLDYFVAEXGLNLVGCCSELNAGYAADGYARRRGV 122

Query: 121 GACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELR 180
           GACAVTFTVGGLSLINAVAGA+SEDLPV+CIVGGPNSNDYGSKKI+HHTIGLPDFSQELR
Sbjct: 123 GACAVTFTVGGLSLINAVAGAYSEDLPVLCIVGGPNSNDYGSKKIIHHTIGLPDFSQELR 182

Query: 181 CFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPL 240
           CFQNVTCYQA+ID++++ QW+ID AICKCL E KPVYIS+CCNLVA PHPSFS++ LIPL
Sbjct: 183 CFQNVTCYQAIIDNVEETQWEIDRAICKCLGERKPVYISICCNLVAIPHPSFSMERLIPL 242

Query: 241 SLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVT 300
           SL PKQSNQMGLE A EKAAQLLNSAVKPV VAGKKLR AKAQAAFVELADACGYAVAVT
Sbjct: 243 SLHPKQSNQMGLEVAAEKAAQLLNSAVKPVMVAGKKLRPAKAQAAFVELADACGYAVAVT 302

Query: 301 PSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNN 360
           PSAKGLFPENHPHFIGTYWG +STAFCGETVEIADAS+FAG T DELETV FSL YKKN 
Sbjct: 303 PSAKGLFPENHPHFIGTYWGAVSTAFCGETVEIADASIFAGATLDELETVAFSLTYKKNK 362

Query: 361 AIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGE 420
           AIIVEPERVVFPNGPSFGPILM DFLRAL +RLKPNPTAYENYRRIYVPESGPLQSEPGE
Sbjct: 363 AIIVEPERVVFPNGPSFGPILMKDFLRALAQRLKPNPTAYENYRRIYVPESGPLQSEPGE 422

Query: 421 ALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATL 480
           ALRVNVLFKHIQKMLSSNM VISEAGDSWFHCQKLKLP+SCGYE+Q++YASIGWSLGATL
Sbjct: 423 ALRVNVLFKHIQKMLSSNMTVISEAGDSWFHCQKLKLPKSCGYEVQLLYASIGWSLGATL 482

Query: 481 GYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNV 540
           GYAQAA  +R+VLC+GDGSFQM+PQD+STMLRLGQ+NIIFLINNGGYTIEVEIHDGPYNV
Sbjct: 483 GYAQAAPQQRVVLCIGDGSFQMSPQDVSTMLRLGQKNIIFLINNGGYTIEVEIHDGPYNV 542

Query: 541 IKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSK 600
           IKNWDYTA+V+AMHN +G CWT KV TEEELVKAI+ A  ++NDCLCFIE I+HRDDTSK
Sbjct: 543 IKNWDYTAMVNAMHNQDGKCWTAKVHTEEELVKAIETATKDRNDCLCFIEAILHRDDTSK 602

Query: 601 ELLEFGNKLAAINSRPPKPL 617
           ELLEFG+K+AAI+ RPP+PL
Sbjct: 603 ELLEFGSKIAAISCRPPRPL 606

BLAST of MC00g0514 vs. ExPASy TrEMBL
Match: A0A5A7T4K4 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00340 PE=3 SV=1)

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 515/630 (81.75%), Postives = 556/630 (88.25%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKP-STNGVLKPLICIQSQLAG-APNIAAAAA 60
           MPINLENG Q E        P    RS LKP +TNGV KP    Q+      P +A  + 
Sbjct: 1   MPINLENGLQAETISSTMPMPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
            PSTLGHYLARRLV+IGI DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGY
Sbjct: 61  VPSTLGHYLARRLVQIGISDIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYG--SKKILHHTIGL 180
           ARRRGVGACAVTFTVGGLSLINA+AGA+SEDLPVVCIVGGPNSN+YG  +KKILHHTIGL
Sbjct: 121 ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGL 180

Query: 181 PDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSF 240
           PDFSQEL+CF+NVTCYQAVI++LKDAQW+ID AI KCLEESKPVYIS+ CNL A PHPSF
Sbjct: 181 PDFSQELQCFRNVTCYQAVINNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSF 240

Query: 241 SIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADA 300
           S+Q LIPLS+ PKQSNQM LE AVEKAAQLLN AVKPV V GKKLR+AKAQAAFVELA+ 
Sbjct: 241 SVQQLIPLSISPKQSNQMSLEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANT 300

Query: 301 CGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGF 360
           C YAVAVTPSAKG+FPENHPHF+GTYWGVLSTAFCGETVEIADAS+F G  FDELETVGF
Sbjct: 301 CDYAVAVTPSAKGMFPENHPHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGF 360

Query: 361 SLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESG 420
           SL YKKN AIIVE + VVFPNGP FGPILM DFL AL KR+KPN TAYENYRRIY+PES 
Sbjct: 361 SLSYKKNKAIIVETDHVVFPNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESS 420

Query: 421 PLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASI 480
           P  SEPGEALRVNVLFKHIQKML+++M VISEAGDSWFH QKL+LPQSCGYEMQI+YASI
Sbjct: 421 PATSEPGEALRVNVLFKHIQKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASI 480

Query: 481 GWSLGATLGYAQAAS--NERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540
           GWSLGATLGYAQA+S  N+R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE
Sbjct: 481 GWSLGATLGYAQASSSSNDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540

Query: 541 VEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIE 600
           VEIHDGPYNVIKNWDY+ALVDAMHN NGNCWTTKV+TEEELVKAI+IA  + NDCLCFIE
Sbjct: 541 VEIHDGPYNVIKNWDYSALVDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIE 600

Query: 601 TIVHRDDTSKELLEFGNKLAAINSRPPKPL 617
            IVHRDDTSKELLEFGNKLA +NSRPP+PL
Sbjct: 601 VIVHRDDTSKELLEFGNKLAEMNSRPPEPL 630

BLAST of MC00g0514 vs. ExPASy TrEMBL
Match: A0A1S3CL30 (Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502191 PE=3 SV=1)

HSP 1 Score: 1030 bits (2662), Expect = 0.0
Identity = 515/630 (81.75%), Postives = 556/630 (88.25%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKP-STNGVLKPLICIQSQLAG-APNIAAAAA 60
           MPINLENG Q E        P    RS LKP +TNGV KP    Q+      P +A  + 
Sbjct: 1   MPINLENGLQAETISSTMPMPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
            PSTLGHYLARRLV+IGI DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGY
Sbjct: 61  VPSTLGHYLARRLVQIGISDIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYG--SKKILHHTIGL 180
           ARRRGVGACAVTFTVGGLSLINA+AGA+SEDLPVVCIVGGPNSN+YG  +KKILHHTIGL
Sbjct: 121 ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGL 180

Query: 181 PDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSF 240
           PDFSQEL+CF+NVTCYQAVI++LKDAQW+ID AI KCLEESKPVYIS+ CNL A PHPSF
Sbjct: 181 PDFSQELQCFRNVTCYQAVINNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSF 240

Query: 241 SIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADA 300
           S+Q LIPLS+ PKQSNQM LE AVEKAAQLLN AVKPV V GKKLR+AKAQAAFVELA+ 
Sbjct: 241 SVQQLIPLSISPKQSNQMSLEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANT 300

Query: 301 CGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGF 360
           C YAVAVTPSAKG+FPENHPHF+GTYWGVLSTAFCGETVEIADAS+F G  FDELETVGF
Sbjct: 301 CDYAVAVTPSAKGMFPENHPHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGF 360

Query: 361 SLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESG 420
           SL YKKN AIIVE + VVFPNGP FGPILM DFL AL KR+KPN TAYENYRRIY+PES 
Sbjct: 361 SLSYKKNKAIIVETDHVVFPNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESS 420

Query: 421 PLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASI 480
           P  SEPGEALRVNVLFKHIQKML+++M VISEAGDSWFH QKL+LPQSCGYEMQI+YASI
Sbjct: 421 PATSEPGEALRVNVLFKHIQKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASI 480

Query: 481 GWSLGATLGYAQAAS--NERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540
           GWSLGATLGYAQA+S  N+R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE
Sbjct: 481 GWSLGATLGYAQASSSSNDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540

Query: 541 VEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIE 600
           VEIHDGPYNVIKNWDY+ALVDAMHN NGNCWTTKV+TEEELVKAI+IA  + NDCLCFIE
Sbjct: 541 VEIHDGPYNVIKNWDYSALVDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIE 600

Query: 601 TIVHRDDTSKELLEFGNKLAAINSRPPKPL 617
            IVHRDDTSKELLEFGNKLA +NSRPP+PL
Sbjct: 601 VIVHRDDTSKELLEFGNKLAEMNSRPPEPL 630

BLAST of MC00g0514 vs. ExPASy TrEMBL
Match: A0A5D3CG96 (Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00020 PE=3 SV=1)

HSP 1 Score: 1029 bits (2660), Expect = 0.0
Identity = 515/630 (81.75%), Postives = 556/630 (88.25%), Query Frame = 0

Query: 1   MPINLENGFQTEN-------PVAPARSKLKP-STNGVLKPLICIQSQLAG-APNIAAAAA 60
           MPINLENG Q E        P    RS LKP +TNGV KP    Q+      P +A  + 
Sbjct: 1   MPINLENGLQAETISSTMPMPTMATRSMLKPPTTNGVYKPHSKSQTFTTNNGPQMADPSV 60

Query: 61  TPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 120
            PSTLGHYLARRLV+IGI DIFSVPGDSNL+LLDYFVAEKGLNLVGCCSELNAGYAADGY
Sbjct: 61  VPSTLGHYLARRLVQIGISDIFSVPGDSNLILLDYFVAEKGLNLVGCCSELNAGYAADGY 120

Query: 121 ARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYG--SKKILHHTIGL 180
           ARRRGVGACAVTFTVGGLSLINA+AGA+SEDLPVVCIVGGPNSN+YG  +KKILHHTIGL
Sbjct: 121 ARRRGVGACAVTFTVGGLSLINAIAGAYSEDLPVVCIVGGPNSNEYGKSNKKILHHTIGL 180

Query: 181 PDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSF 240
           PDFSQEL+CF+NVTCYQAVI++LKDAQW+ID AI KCLEESKPVYIS+ CNL A PHPSF
Sbjct: 181 PDFSQELQCFRNVTCYQAVINNLKDAQWEIDCAISKCLEESKPVYISIACNLAAIPHPSF 240

Query: 241 SIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADA 300
           S+Q LIPLS+ PKQSNQM LE AVEKAAQLLN AVKPV V GKKLR+AKAQAAFVELA+ 
Sbjct: 241 SMQQLIPLSISPKQSNQMSLEMAVEKAAQLLNGAVKPVLVGGKKLRVAKAQAAFVELANT 300

Query: 301 CGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGF 360
           C YAVAVTPSAKG+FPENHPHF+GTYWGVLSTAFCGETVEIADAS+F G  FDELETVGF
Sbjct: 301 CDYAVAVTPSAKGMFPENHPHFMGTYWGVLSTAFCGETVEIADASIFVGAIFDELETVGF 360

Query: 361 SLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESG 420
           SL YKKN AIIVE + VVFPNGP FGPILM DFL AL KR+KPN TAYENYRRIY+PES 
Sbjct: 361 SLSYKKNKAIIVETDHVVFPNGPRFGPILMKDFLWALAKRVKPNSTAYENYRRIYIPESS 420

Query: 421 PLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASI 480
           P  SEPGEALRVNVLFKHIQKML+++M VISEAGDSWFH QKL+LPQSCGYEMQI+YASI
Sbjct: 421 PATSEPGEALRVNVLFKHIQKMLTNDMTVISEAGDSWFHTQKLRLPQSCGYEMQIVYASI 480

Query: 481 GWSLGATLGYAQAAS--NERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540
           GWSLGATLGYAQA+S  N+R+VLC+GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE
Sbjct: 481 GWSLGATLGYAQASSSSNDRVVLCIGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIE 540

Query: 541 VEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIE 600
           VEIHDGPYNVIKNWDY+ALVDAMHN NGNCWTTKV+TEEELVKAI+IA  + NDCLCFIE
Sbjct: 541 VEIHDGPYNVIKNWDYSALVDAMHNHNGNCWTTKVQTEEELVKAIEIATRDHNDCLCFIE 600

Query: 601 TIVHRDDTSKELLEFGNKLAAINSRPPKPL 617
            IVHRDDTSKELLEFGNKLA +NSRPP+PL
Sbjct: 601 VIVHRDDTSKELLEFGNKLAEMNSRPPEPL 630

BLAST of MC00g0514 vs. ExPASy TrEMBL
Match: A0A6J1IP68 (Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478194 PE=3 SV=1)

HSP 1 Score: 1018 bits (2631), Expect = 0.0
Identity = 496/604 (82.12%), Postives = 546/604 (90.40%), Query Frame = 0

Query: 14  PVAPARSKLKPST-NGVLKPLICIQSQLAG-APNIAAAAATPSTLGHYLARRLVEIGIGD 73
           P+A  RS LKP T NG  KP   +Q+        I  +  TPSTLGHYLARRLV+IG+ D
Sbjct: 2   PMAAVRSMLKPPTSNGSYKPQSKLQTNNCNNGVEIVESMVTPSTLGHYLARRLVQIGVSD 61

Query: 74  IFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSL 133
           IFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVG+C VTFTVGGLSL
Sbjct: 62  IFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGSCVVTFTVGGLSL 121

Query: 134 INAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDS 193
           INA+AGA+SEDLP+VCIVG PNSN+YGS KILHHTIGLPDFSQELRCFQNVTCYQAVI++
Sbjct: 122 INAIAGAYSEDLPLVCIVGAPNSNEYGSNKILHHTIGLPDFSQELRCFQNVTCYQAVINN 181

Query: 194 LKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEA 253
           L+DAQW+IDTAI KCLE+SKPVYIS+ CNL A PHPSFS++  +PLS+  KQ+NQMGLE 
Sbjct: 182 LEDAQWEIDTAISKCLEQSKPVYISISCNLAAIPHPSFSMKQFLPLSISSKQTNQMGLEM 241

Query: 254 AVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHF 313
           AVE AAQ+LN+AVKP+ V GKKLR+AKAQAAFVELAD C YAVAVTPSAKG+FPENH HF
Sbjct: 242 AVEMAAQILNTAVKPIMVGGKKLRVAKAQAAFVELADVCDYAVAVTPSAKGMFPENHRHF 301

Query: 314 IGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNG 373
           IGTYWG LS+AFCGETVEIADAS+FAG  FDELETVG+SL YKKN AIIVE +RVVFPNG
Sbjct: 302 IGTYWGSLSSAFCGETVEIADASIFAGAIFDELETVGYSLSYKKNKAIIVESDRVVFPNG 361

Query: 374 PSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKM 433
           P FGPILM DFLRAL KRLKPNPTAYENY RIY+P+SGP QSEPGEALRVNVLFKHIQKM
Sbjct: 362 PIFGPILMKDFLRALAKRLKPNPTAYENYTRIYIPKSGPTQSEPGEALRVNVLFKHIQKM 421

Query: 434 LSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLC 493
           LS NMAVISEAGDSWFHCQKL+LP+SCG+EMQ++YASIGWSLGATLGYAQA+S++R+VLC
Sbjct: 422 LSGNMAVISEAGDSWFHCQKLELPRSCGFEMQLVYASIGWSLGATLGYAQASSDDRVVLC 481

Query: 494 MGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMH 553
           +GDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYT LV+AMH
Sbjct: 482 IGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTGLVEAMH 541

Query: 554 NANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINS 613
           N NGNCWTTKV+TEEELVKAI++A G+KN+CLCFIE IVHRDDTSKELL+FGNKLAA NS
Sbjct: 542 NQNGNCWTTKVETEEELVKAIEMATGDKNECLCFIEAIVHRDDTSKELLDFGNKLAATNS 601

Query: 614 RPPK 615
           RPPK
Sbjct: 602 RPPK 605

BLAST of MC00g0514 vs. TAIR 10
Match: AT5G01320.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 812.4 bits (2097), Expect = 2.5e-235
Identity = 392/594 (65.99%), Postives = 471/594 (79.29%), Query Frame = 0

Query: 23  KPSTNGVLKPLICIQSQLAGAPNIAAAAATPSTLGHYLARRLVEIGIGDIFSVPGDSNLV 82
           KP+T  +  P     + +  +  I     + STLG +L+RRLV+ G+ D+FSVPGD NL 
Sbjct: 12  KPTTGDIGSPPSNAVATIQDSAPI--TTTSESTLGRHLSRRLVQAGVTDVFSVPGDFNLT 71

Query: 83  LLDYFVAEKGLNLVGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVAGAFSED 142
           LLD+ +AE  LN +GCC+ELNAGYAADGYAR RGVGAC VTFTVGGLS++NA+AGA+SE+
Sbjct: 72  LLDHLIAEPELNNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSEN 131

Query: 143 LPVVCIVGGPNSNDYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTA 202
           LPV+CIVGGPNSND+G+ +ILHHTIGLPDFSQELRCFQ VTCYQAV+++L+DA  +ID A
Sbjct: 132 LPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEQIDKA 191

Query: 203 ICKCLEESKPVYISVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNS 262
           I   L+ESKPVYIS+ CNL ATPHP+F+ +  +P  L P+ SN MGLEAAVE   + LN 
Sbjct: 192 IATALKESKPVYISISCNLAATPHPTFA-RDPVPFDLTPRMSNTMGLEAAVEATLEFLNK 251

Query: 263 AVKPVAVAGKKLRLAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTA 322
           AVKPV V G KLR+AKA  AF+ELADA GY +AV PS KGL PENHPHFIGTYWG +ST 
Sbjct: 252 AVKPVMVGGPKLRVAKASEAFLELADASGYPLAVMPSTKGLVPENHPHFIGTYWGAVSTP 311

Query: 323 FCGETVEIADASLFAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNGPSFGPILMMDF 382
           FC E VE ADA +FAGP F++  +VG+SL  KK  AIIV P+RVV  NGP+FG +LM DF
Sbjct: 312 FCSEIVESADAYIFAGPIFNDYSSVGYSLLLKKEKAIIVHPDRVVVANGPTFGCVLMSDF 371

Query: 383 LRALTKRLKPNPTAYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMAVISEA 442
            R L KR+K N TAYENY RI+VPE  PL+ +PGE LRVN +F+HIQKMLSS  AVI+E 
Sbjct: 372 FRELAKRVKRNETAYENYERIFVPEGKPLKCKPGEPLRVNAMFQHIQKMLSSETAVIAET 431

Query: 443 GDSWFHCQKLKLPQSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLCMGDGSFQMAPQ 502
           GDSWF+CQKLKLP+ CGYE Q+ Y SIGWS+GATLGYAQA   +R++  +GDGSFQ+  Q
Sbjct: 432 GDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQ 491

Query: 503 DLSTMLRLGQRNIIFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKV 562
           D+STM+R GQ+ IIFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+HN  G CWTTKV
Sbjct: 492 DISTMIRNGQKAIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKV 551

Query: 563 KTEEELVKAIQIAMGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINSRPPKP 617
           + EEELV+AI+ A  EK D LCFIE IVH+DDTSKELLE+G++++A N RPP P
Sbjct: 552 RYEEELVEAIKTATTEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNP 602

BLAST of MC00g0514 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 810.8 bits (2093), Expect = 7.4e-235
Identity = 388/563 (68.92%), Postives = 462/563 (82.06%), Query Frame = 0

Query: 54  STLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYAR 113
           +TLG +LARRLV+ G+ D+FSVPGD NL LLD+ +AE  LNL+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRHLARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYAR 104

Query: 114 RRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFS 173
            RGVGAC VTFTVGGLS++NA+AGA+SE+LP++CIVGGPNSNDYG+ +ILHHTIGLPDFS
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFS 164

Query: 174 QELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQS 233
           QELRCFQ VTCYQAV+++L DA  +ID AI   L+ESKPVYISV CNL A PH +FS + 
Sbjct: 165 QELRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKPVYISVSCNLAAIPHHTFS-RD 224

Query: 234 LIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYA 293
            +P SL P+ SN+MGLEAAVE   + LN AVKPV V G KLR+AKA  AFVELADA GYA
Sbjct: 225 PVPFSLAPRLSNKMGLEAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELADASGYA 284

Query: 294 VAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCY 353
           +A+ PSAKG  PE+HPHFIGTYWG +ST FC E VE ADA +FAGP F++  +VG+SL  
Sbjct: 285 LAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVGYSLLL 344

Query: 354 KKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQS 413
           KK  AI+V+P+R+   NGP+FG ILM DF R L+KR+K N TAYENY RI+VPE  PL+ 
Sbjct: 345 KKEKAIVVQPDRITVANGPTFGCILMSDFFRELSKRVKRNETAYENYHRIFVPEGKPLKC 404

Query: 414 EPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSL 473
           E  E LRVN +F+HIQKMLSS  AVI+E GDSWF+CQKLKLP+ CGYE Q+ Y SIGWS+
Sbjct: 405 ESREPLRVNTMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSV 464

Query: 474 GATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDG 533
           GATLGYAQA+  +R++  +GDGSFQ+  QD+STMLR GQ+ IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQASPEKRVLAFIGDGSFQVTVQDISTMLRNGQKTIIFLINNGGYTIEVEIHDG 524

Query: 534 PYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRD 593
           PYNVIKNW+YT LVDA+HN  GNCWT KV+ EEELV+AI  A  EK DCLCFIE I+H+D
Sbjct: 525 PYNVIKNWNYTGLVDAIHNGEGNCWTAKVRYEEELVEAITTATTEKKDCLCFIEVILHKD 584

Query: 594 DTSKELLEFGNKLAAINSRPPKP 617
           DTSKELLE+G++++A NSRPP P
Sbjct: 585 DTSKELLEWGSRVSAANSRPPNP 606

BLAST of MC00g0514 vs. TAIR 10
Match: AT5G54960.1 (pyruvate decarboxylase-2 )

HSP 1 Score: 810.1 bits (2091), Expect = 1.3e-234
Identity = 387/563 (68.74%), Postives = 460/563 (81.71%), Query Frame = 0

Query: 54  STLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGYAR 113
           +TLG YLARRLVEIG+ D+FSVPGD NL LLD+ +AE  L L+GCC+ELNAGYAADGYAR
Sbjct: 45  ATLGRYLARRLVEIGVTDVFSVPGDFNLTLLDHLIAEPNLKLIGCCNELNAGYAADGYAR 104

Query: 114 RRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSNDYGSKKILHHTIGLPDFS 173
            RGVGAC VTFTVGGLS++NA+AGA+SE+LP++CIVGGPNSNDYG+ +ILHHTIGLPDF+
Sbjct: 105 SRGVGACVVTFTVGGLSVLNAIAGAYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFT 164

Query: 174 QELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYISVCCNLVATPHPSFSIQS 233
           QELRCFQ VTC+QAVI++L++A   IDTAI   L+ESKPVYIS+ CNL A P P+FS + 
Sbjct: 165 QELRCFQAVTCFQAVINNLEEAHELIDTAISTALKESKPVYISISCNLPAIPLPTFS-RH 224

Query: 234 LIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQAAFVELADACGYA 293
            +P  LP K SNQ+GL+AAVE AA+ LN AVKPV V G K+R+AKA  AFVELADA GY 
Sbjct: 225 PVPFMLPMKVSNQIGLDAAVEAAAEFLNKAVKPVLVGGPKMRVAKAADAFVELADASGYG 284

Query: 294 VAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTFDELETVGFSLCY 353
           +AV PSAKG  PE+H HFIGTYWG +STAFC E VE ADA LFAGP F++  +VG+SL  
Sbjct: 285 LAVMPSAKGQVPEHHKHFIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLL 344

Query: 354 KKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPTAYENYRRIYVPESGPLQS 413
           KK  AIIV+P+RV   NGP+FG +LM DFL  L KR+K N T+YENY RIYVPE  PL+ 
Sbjct: 345 KKEKAIIVQPDRVTIGNGPAFGCVLMKDFLSELAKRIKHNNTSYENYHRIYVPEGKPLRD 404

Query: 414 EPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLPQSCGYEMQIIYASIGWSL 473
            P E+LRVNVLF+HIQ MLSS  AV++E GDSWF+CQKLKLP+ CGYE Q+ Y SIGWS+
Sbjct: 405 NPNESLRVNVLFQHIQNMLSSESAVLAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSV 464

Query: 474 GATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNIIFLINNGGYTIEVEIHDG 533
           GATLGYAQA  N R++ C+GDGSFQ+  QD+STM+R GQ+ IIFLINNGGYTIEVEIHDG
Sbjct: 465 GATLGYAQAMPNRRVIACIGDGSFQVTAQDVSTMIRCGQKTIIFLINNGGYTIEVEIHDG 524

Query: 534 PYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIAMGEKNDCLCFIETIVHRD 593
           PYNVIKNW+YTA V+A+HN  G CWT KV+ EEELVKAI  A  E+ +  CFIE IVH+D
Sbjct: 525 PYNVIKNWNYTAFVEAIHNGEGKCWTAKVRCEEELVKAINTATNEEKESFCFIEVIVHKD 584

Query: 594 DTSKELLEFGNKLAAINSRPPKP 617
           DTSKELLE+G++++A NSRPP P
Sbjct: 585 DTSKELLEWGSRVSAANSRPPNP 606

BLAST of MC00g0514 vs. TAIR 10
Match: AT5G01330.1 (pyruvate decarboxylase-3 )

HSP 1 Score: 790.8 bits (2041), Expect = 7.9e-229
Identity = 384/581 (66.09%), Postives = 458/581 (78.83%), Query Frame = 0

Query: 36  IQSQLAGAPNIAAAAATPSTLGHYLARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNL 95
           IQ     A  I  ++A  +TLG +L+RRLV+ G+ DIF+VPGD NL LLD  +A   LN 
Sbjct: 14  IQDSAPTAATILGSSA--ATLGRHLSRRLVQAGVTDIFTVPGDFNLSLLDQLIANPELNN 73

Query: 96  VGCCSELNAGYAADGYARRRGVGACAVTFTVGGLSLINAVAGAFSEDLPVVCIVGGPNSN 155
           +GCC+ELNAGYAADGYAR RGVGAC VTFTVGGLS++NA+AGA+SE+LPV+CIVGGPNSN
Sbjct: 74  IGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVGGPNSN 133

Query: 156 DYGSKKILHHTIGLPDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEESKPVYI 215
           D+G+ +ILHHTIGLPDFSQELRCFQ VTCYQAV++ L+DA  +ID AI   L ESKPVYI
Sbjct: 134 DFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNHLEDAHEQIDKAIATALRESKPVYI 193

Query: 216 SVCCNLVATPHPSFSIQSLIPLSLPPKQSNQMGLEAAVEKAAQLLNSAVKPVAVAGKKLR 275
           S+ CNL A PHP+F+    +P  L P+ SN+  LEAAVE   + LN AVKPV V G KLR
Sbjct: 194 SISCNLAAIPHPTFASYP-VPFDLTPRLSNKDCLEAAVEATLEFLNKAVKPVMVGGPKLR 253

Query: 276 LAKAQAAFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASL 335
           +AKA+ AFVELADA GY VAV PSAKG  PENHPHFIGTYWG +ST FC E VE ADA +
Sbjct: 254 VAKARDAFVELADASGYPVAVMPSAKGFVPENHPHFIGTYWGAVSTLFCSEIVESADAYI 313

Query: 336 FAGPTFDELETVGFSLCYKKNNAIIVEPERVVFPNGPSFGPILMMDFLRALTKRLKPNPT 395
           FAGP F++  +VG+SL  KK  AIIV P+ VV  NGP+FG + M +F R L KR+KPN T
Sbjct: 314 FAGPIFNDYSSVGYSLLLKKEKAIIVHPDSVVVANGPTFGCVRMSEFFRELAKRVKPNKT 373

Query: 396 AYENYRRIYVPESGPLQSEPGEALRVNVLFKHIQKMLSSNMAVISEAGDSWFHCQKLKLP 455
           AYENY RI+VPE  PL+ +P E LR+N +F+HIQKMLS+  AVI+E GDSWF+CQKLKLP
Sbjct: 374 AYENYHRIFVPEGKPLKCKPREPLRINAMFQHIQKMLSNETAVIAETGDSWFNCQKLKLP 433

Query: 456 QSCGYEMQIIYASIGWSLGATLGYAQAASNERMVLCMGDGSFQMAPQDLSTMLRLGQRNI 515
           + CGYE Q+ Y SIGWS+GATLGYAQA   +R++  +GDGSFQ+  QD+STM+R GQ+ I
Sbjct: 434 KGCGYEFQMQYGSIGWSVGATLGYAQATPEKRVLSFIGDGSFQVTAQDVSTMIRNGQKTI 493

Query: 516 IFLINNGGYTIEVEIHDGPYNVIKNWDYTALVDAMHNANGNCWTTKVKTEEELVKAIQIA 575
           IFLINNGGYTIEVEIHDGPYNVIKNW+YT LVDA+HN  G CWTTKV+ EEELV+AI  A
Sbjct: 494 IFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEGKCWTTKVRYEEELVEAINTA 553

Query: 576 MGEKNDCLCFIETIVHRDDTSKELLEFGNKLAAINSRPPKP 617
             EK D LCFIE IVH+DDTSKELLE+G++++A N RPP P
Sbjct: 554 TLEKKDSLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNP 591

BLAST of MC00g0514 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 76.6 bits (187), Expect = 7.6e-14
Identity = 115/501 (22.95%), Postives = 199/501 (39.72%), Query Frame = 0

Query: 53  PSTLGHYL-ARRLVEIGIGDIFSVPGDSNLVLLDYFVAEKGLNLVGCCSELNAGYAADGY 112
           PS  G+ L A+ L  +G+  +F V G     L    +A  G+  +   +E +AGYAA  Y
Sbjct: 10  PSIDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMA-LGIRFIAFHNEQSAGYAASAY 69

Query: 113 ARRRGVGACAVTFTVGGLSLINAVAG---AFSEDLPVVCIVGGPNSNDYGSKKILHHTIG 172
                 G   +  TV G   ++ +AG   A+    P+V I G  +  D          +G
Sbjct: 70  GYL--TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRD----------VG 129

Query: 173 LPDFSQELRCFQNVTCYQAVIDSLKDAQWKIDTAICKCLEES---KP--VYISVCCNLVA 232
             DF QEL   + V  +  + +  KD + +I   + + L+ +   +P   Y+ +  +++ 
Sbjct: 130 RGDF-QELDQIEAVKAFSKLSEKAKDVR-EIPDCVSRVLDRAVSGRPGGCYLDIPTDVLR 189

Query: 233 TPHPSFSIQSLIPLSLPPKQSNQM--GLEAAVEKAAQLLNSAVKPVAVAGKKLRLAKAQA 292
                     L+      ++   +   L + +E A  LL  A +P+ V GK    ++A+ 
Sbjct: 190 QKISESEADKLVDEVERSRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAED 249

Query: 293 AFVELADACGYAVAVTPSAKGLFPENHPHFIGTYWGVLSTAFCGETVEIADASLFAGPTF 352
              +L +  G     TP  KGL P+ H  F  T    L+   C       D +L  G   
Sbjct: 250 ELKKLVEITGIPFLPTPMGKGLLPDTH-EFSATAARSLAIGKC-------DVALVVGARL 309

Query: 353 DELETVGFSLCYKKNNAIIV--EPERVVFPNGPSFGPI-LMMDFLRALTKRLKPNPTAYE 412
           + L   G S  + K+   I+    E  +    P  G +      +  L + +K +P    
Sbjct: 310 NWLLHFGESPKWDKDVKFILVDVSEEEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCL- 369

Query: 413 NYRRIYVPESGPLQSEPGEALRVN-----VLFKHIQKMLSSNMAVISEAGDSWFHCQKLK 472
                +V        E GE + +      V F  +  M     A+++  G S     +  
Sbjct: 370 GKSNSWVESISKKAKENGEKMEIQLAKDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGA 429

Query: 473 LPQSCGYEMQI-------IYASIGWSLGATLGY---AQAASNERMVLCM-GDGSFQMAPQ 524
                G  + +       + A    ++G  LGY   A  AS +R+V+ + GD  F  +  
Sbjct: 430 NTMDVGRSVLVQKEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAM 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A2XFI31.2e-23768.07Pyruvate decarboxylase 2 OS=Oryza sativa subsp. indica OX=39946 GN=PDC2 PE=2 SV=... [more]
A2Y5L92.9e-23665.83Pyruvate decarboxylase 1 OS=Oryza sativa subsp. indica OX=39946 GN=PDC1 PE=2 SV=... [more]
Q10MW33.8e-23668.07Pyruvate decarboxylase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC2 PE=2 S... [more]
P285166.5e-23665.45Pyruvate decarboxylase 1 OS=Zea mays OX=4577 GN=PDC1 PE=2 SV=1[more]
Q0DHF66.5e-23665.67Pyruvate decarboxylase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PDC1 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_022144001.10.082.58pyruvate decarboxylase 1-like [Momordica charantia][more]
XP_023544035.10.081.57pyruvate decarboxylase 1-like [Cucurbita pepo subsp. pepo][more]
XP_008464266.10.081.75PREDICTED: pyruvate decarboxylase 1-like [Cucumis melo] >KAA0037738.1 pyruvate d... [more]
TYK10923.10.081.75pyruvate decarboxylase 1-like [Cucumis melo var. makuwa][more]
XP_038882338.10.081.25pyruvate decarboxylase 1-like, partial [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1CS040.082.58Pyruvate decarboxylase OS=Momordica charantia OX=3673 GN=LOC111013786 PE=3 SV=1[more]
A0A5A7T4K40.081.75Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3CL300.081.75Pyruvate decarboxylase OS=Cucumis melo OX=3656 GN=LOC103502191 PE=3 SV=1[more]
A0A5D3CG960.081.75Pyruvate decarboxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A6J1IP680.082.12Pyruvate decarboxylase OS=Cucurbita maxima OX=3661 GN=LOC111478194 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G01320.12.5e-23565.99Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT4G33070.17.4e-23568.92Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT5G54960.11.3e-23468.74pyruvate decarboxylase-2 [more]
AT5G01330.17.9e-22966.09pyruvate decarboxylase-3 [more]
AT5G17380.17.6e-1422.95Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePIRSFPIRSF036565Pyruvt_ip_decrbcoord: 49..612
e-value: 9.1E-190
score: 629.5
IPR012110Thiamine pyrophosphate (TPP)-dependent enzymePANTHERPTHR43452PYRUVATE DECARBOXYLASEcoord: 44..616
NoneNo IPR availableGENE3D3.40.50.970coord: 51..230
e-value: 1.2E-56
score: 193.1
NoneNo IPR availableGENE3D3.40.50.1220coord: 242..405
e-value: 1.1E-38
score: 134.3
NoneNo IPR availableGENE3D3.40.50.970coord: 411..603
e-value: 4.4E-51
score: 174.9
NoneNo IPR availablePIRSRPIRSR036565-2PIRSR036565-2coord: 55..599
e-value: 1.4E-162
score: 539.6
NoneNo IPR availablePANTHERPTHR43452:SF25PYRUVATE DECARBOXYLASE 1coord: 44..616
NoneNo IPR availableCDDcd07038TPP_PYR_PDC_IPDC_likecoord: 59..219
e-value: 5.69338E-85
score: 260.89
NoneNo IPR availableCDDcd02005TPP_PDC_IPDCcoord: 418..600
e-value: 3.19147E-76
score: 238.973
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 253..359
e-value: 2.4E-20
score: 72.7
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 462..584
e-value: 2.2E-14
score: 53.5
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 55..226
e-value: 1.2E-31
score: 109.6
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 55..229
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 420..612
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 248..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC00g0514.1MC00g0514.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004737 pyruvate decarboxylase activity
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0016831 carboxy-lyase activity
molecular_function GO:0003824 catalytic activity