MC00g0495 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0495
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	macrophage migration inhibitory factor homolog
Location	scaffold196: 451098 .. 451976 (+)
RNA-Seq Expression	MC00g0495
Synteny	MC00g0495

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAATATAATCCTTCCTCTTAATTAATTTGTTCGTGAAATCAAATCGTCGCCACCAACCACACCATGCCTTGCCTTTACATCTCCACCAACGTCGACTTGGCCGGAGCCGACACCGCCGCCATCTTCTCCGCCACCACCACCGCCGTCTCCTCCATTATCGGCAAACCCGAAAACGTAACACCCTCTCTCCTCTCCTCCTCCTTCCTCCCAGACAAATACCTAAAGGCTTGAACAGATGGATTTGATAAATTTAATTCGTATATTGGATATCTAACTTTTTGTTTTGTTTGCGTCCAGTTTGTGATGGTGTTGCTGAAAGGGTCGGTGGCGATATCGTTCGGAGGGAACGGGGAGCCGGCGGCGTTCGCGGAGGTGGTGTCCATGGGGGGAATAAACTCGCAGGTGAAGAGGAACCTGATCTCCACCATTGCCGCTATTTTGAACGATAAACTTTCGGTTCCGCCGGCGAGATTCTTCCTTAAAGTCCACGACACCACCGCTGGTCGACCCATTTCCAAATTATGACTCCTCCGGCCACGGCGACTGCGACGGCTGCCATTGTACCGGTTTTTATATTATTAGTTTGACTTTTTTTAATTATTATTTTTTTTTTGTCGTGACAGTTAATTGGAGTTGTGTAGTTGAAAAATAATTCAAACGCCTATGATGGCTATGACTATGAAAATGTGAGAGAGAAAAGAAGAAAGAGTCCTCTTTATCGCTTTTGTATTTGATGGGTTATAAAATTAATATAACCATATATACATATTTTGAATATAACAAATATCGTGCGGATTGAATTCAGATTCACAACTTAGAATTAGTTTCCTTTTAATAATTAGTTATTGTTTTTGGCTAATAATATAACGAAATCTAGC

mRNA sequence

CAAATATAATCCTTCCTCTTAATTAATTTGTTCGTGAAATCAAATCGTCGCCACCAACCACACCATGCCTTGCCTTTACATCTCCACCAACGTCGACTTGGCCGGAGCCGACACCGCCGCCATCTTCTCCGCCACCACCACCGCCGTCTCCTCCATTATCGGCAAACCCGAAAACTTTGTGATGGTGTTGCTGAAAGGGTCGGTGGCGATATCGTTCGGAGGGAACGGGGAGCCGGCGGCGTTCGCGGAGGTGGTGTCCATGGGGGGAATAAACTCGCAGGTGAAGAGGAACCTGATCTCCACCATTGCCGCTATTTTGAACGATAAACTTTCGGTTCCGCCGGCGAGATTCTTCCTTAAAGTCCACGACACCACCGCTGGTCGACCCATTTCCAAATTATGACTCCTCCGGCCACGGCGACTGCGACGGCTGCCATTGTACCGGTTTTTATATTATTAGTTTGACTTTTTTTAATTATTATTTTTTTTTTGTCGTGACAGTTAATTGGAGTTGTGTAGTTGAAAAATAATTCAAACGCCTATGATGGCTATGACTATGAAAATGTGAGAGAGAAAAGAAGAAAGAGTCCTCTTTATCGCTTTTGTATTTGATGGGTTATAAAATTAATATAACCATATATACATATTTTGAATATAACAAATATCGTGCGGATTGAATTCAGATTCACAACTTAGAATTAGTTTCCTTTTAATAATTAGTTATTGTTTTTGGCTAATAATATAACGAAATCTAGC

Coding sequence (CDS)

ATGCCTTGCCTTTACATCTCCACCAACGTCGACTTGGCCGGAGCCGACACCGCCGCCATCTTCTCCGCCACCACCACCGCCGTCTCCTCCATTATCGGCAAACCCGAAAACTTTGTGATGGTGTTGCTGAAAGGGTCGGTGGCGATATCGTTCGGAGGGAACGGGGAGCCGGCGGCGTTCGCGGAGGTGGTGTCCATGGGGGGAATAAACTCGCAGGTGAAGAGGAACCTGATCTCCACCATTGCCGCTATTTTGAACGATAAACTTTCGGTTCCGCCGGCGAGATTCTTCCTTAAAGTCCACGACACCACCGCTGGTCGACCCATTTCCAAATTATGA

Protein sequence

MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAFAEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL

Homology

[Close]

BLAST of MC00g0495 vs. ExPASy Swiss-Prot
Match: P81748 (Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 9.3e-08
Identity = 31/102 (30.39%), Postives = 54/102 (52.94%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MP    STNV      +     +T+  ++ ++GKPE++V V + G   I+FGG   PA F
Sbjct: 1   MPIFTFSTNVPSENI-SVDFLKSTSKLIAGMLGKPESYVAVHINGGQKITFGGTDAPAGF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHD 103
            +++S+GG+  +  R+  + +   L D L +P  R ++   D
Sbjct: 61  GQLLSLGGVGGEKNRSHSAKLFKHLTDGLGIPGNRMYINFVD 101

[Close]

BLAST of MC00g0495 vs. ExPASy Swiss-Prot
Match: P81529 (Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 PE=1 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-06
Identity = 31/98 (31.63%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 1  MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
          MP   ++TN+       +   S+T+  V +I+ KP ++V V +     +SFGG+ +PAAF
Sbjct: 1  MPIFTLNTNIKATDV-PSDFLSSTSALVGNILSKPGSYVAVHINTDQQLSFGGSTKPAAF 60

Query: 61 AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFL 99
            ++S+GGI     R+  + +   LN KL +P  R ++
Sbjct: 61 GTLMSIGGIEPSRNRDHSAKLFDHLNKKLGIPKNRMYI 97

[Close]

BLAST of MC00g0495 vs. ExPASy Swiss-Prot
Match: P91850 (Macrophage migration inhibitory factor homolog OS=Brugia malayi OX=6279 GN=Bm1_28435 PE=3 SV=4)

HSP 1 Score: 51.6 bits (122), Expect = 6.7e-06
Identity = 29/105 (27.62%), Postives = 55/105 (52.38%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MP   I TN+    + ++A     +  V+  +GKPE++V + + G  A+ FGG+ +P A 
Sbjct: 1   MPYFTIDTNIP-QNSISSAFLKKASNVVAKALGKPESYVSIHVNGGQAMVFGGSEDPCAV 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTA 106
             + S+G +  +V  +    +  +L D+L +P  R +++  D  A
Sbjct: 61  CVLKSIGCVGPKVNNSHAEKLYKLLADELKIPKNRCYIEFVDIEA 104

[Close]

BLAST of MC00g0495 vs. ExPASy Swiss-Prot
Match: P80177 (Macrophage migration inhibitory factor OS=Bos taurus OX=9913 GN=MIF PE=1 SV=6)

HSP 1 Score: 48.9 bits (115), Expect = 4.3e-05
Identity = 28/105 (26.67%), Postives = 49/105 (46.67%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MP   ++TNV  A      + S  T  ++   GKP  ++ V +     ++FGG+ EP A 
Sbjct: 1   MPMFVVNTNVPRASVPD-GLLSELTQQLAQATGKPAQYIAVHVVPDQLMTFGGSSEPCAL 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTA 106
             + S+G I     R+    +  +L ++L + P R ++   D  A
Sbjct: 61  CSLHSIGKIGGAQNRSYSKLLCGLLTERLRISPDRIYINFCDMNA 104

[Close]

BLAST of MC00g0495 vs. ExPASy Swiss-Prot
Match: Q1ZZU7 (Macrophage migration inhibitory factor OS=Ovis aries OX=9940 GN=MIF PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 4.3e-05
Identity = 28/105 (26.67%), Postives = 49/105 (46.67%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MP   ++TNV  A      + S  T  ++   GKP  ++ V +     ++FGG+ EP A 
Sbjct: 1   MPMFVVNTNVPRASVPD-GLLSELTQQLAQATGKPAQYIAVHVVPDQLMTFGGSSEPCAL 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTA 106
             + S+G I     R+    +  +L ++L + P R ++   D  A
Sbjct: 61  CSLHSIGKIGGAQNRSYSKLLCGLLTERLRISPDRIYINFCDMNA 104

[Close]

BLAST of MC00g0495 vs. NCBI nr
Match: XP_022144090.1 (macrophage migration inhibitory factor homolog [Momordica charantia])

HSP 1 Score: 214 bits (546), Expect = 5.53e-70
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF
Sbjct: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. NCBI nr
Match: XP_038883562.1 (macrophage migration inhibitory factor homolog [Benincasa hispida])

HSP 1 Score: 198 bits (503), Expect = 2.00e-63
Identity = 101/112 (90.18%), Postives = 108/112 (96.43%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNV+LAGAD+ AIFSATTTAVSSIIGKPEN+VMVLLKGSV ISFGGNGEPAAF
Sbjct: 1   MPCLYISTNVNLAGADSTAIFSATTTAVSSIIGKPENYVMVLLKGSVPISFGGNGEPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. NCBI nr
Match: XP_022949815.1 (macrophage migration inhibitory factor homolog [Cucurbita moschata] >XP_023543101.1 macrophage migration inhibitory factor homolog [Cucurbita pepo subsp. pepo] >KAG6603558.1 hypothetical protein SDJN03_04167, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033748.1 hypothetical protein SDJN02_03473, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 194 bits (494), Expect = 4.72e-62
Identity = 99/112 (88.39%), Postives = 108/112 (96.43%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNVDLAGAD+AAIF+ATT+AVSSIIGKPEN+VMVLLKGSVAISFGGN EPAAF
Sbjct: 1   MPCLYISTNVDLAGADSAAIFAATTSAVSSIIGKPENYVMVLLKGSVAISFGGNKEPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LI+TI +IL +KLSVPPARFFLKVHDTTAGRP+SKL
Sbjct: 61  AEVVSMGGINSEVKRRLIATIGSILKEKLSVPPARFFLKVHDTTAGRPVSKL 112

[Close]

BLAST of MC00g0495 vs. NCBI nr
Match: XP_008464238.1 (PREDICTED: macrophage migration inhibitory factor homolog [Cucumis melo])

HSP 1 Score: 193 bits (491), Expect = 1.35e-61
Identity = 98/112 (87.50%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNV+LAG D+AAIFSATTTAVSSIIGKPEN+VMVLL GSV ISFGGNG+PA F
Sbjct: 1   MPCLYISTNVNLAGVDSAAIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAVF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. NCBI nr
Match: XP_004148599.1 (macrophage migration inhibitory factor homolog [Cucumis sativus])

HSP 1 Score: 193 bits (490), Expect = 1.92e-61
Identity = 98/112 (87.50%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNV+LAG D+A IFSATTTAVSSIIGKPEN+VMVLL GSV ISFGGNG+PAAF
Sbjct: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. ExPASy TrEMBL
Match: A0A6J1CQN7 (macrophage migration inhibitory factor homolog OS=Momordica charantia OX=3673 GN=LOC111013868 PE=3 SV=1)

HSP 1 Score: 214 bits (546), Expect = 2.68e-70
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF
Sbjct: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. ExPASy TrEMBL
Match: A0A6J1GDU8 (macrophage migration inhibitory factor homolog OS=Cucurbita moschata OX=3662 GN=LOC111453099 PE=3 SV=1)

HSP 1 Score: 194 bits (494), Expect = 2.29e-62
Identity = 99/112 (88.39%), Postives = 108/112 (96.43%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNVDLAGAD+AAIF+ATT+AVSSIIGKPEN+VMVLLKGSVAISFGGN EPAAF
Sbjct: 1   MPCLYISTNVDLAGADSAAIFAATTSAVSSIIGKPENYVMVLLKGSVAISFGGNKEPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LI+TI +IL +KLSVPPARFFLKVHDTTAGRP+SKL
Sbjct: 61  AEVVSMGGINSEVKRRLIATIGSILKEKLSVPPARFFLKVHDTTAGRPVSKL 112

[Close]

BLAST of MC00g0495 vs. ExPASy TrEMBL
Match: A0A1S3CL09 (macrophage migration inhibitory factor homolog OS=Cucumis melo OX=3656 GN=LOC103502168 PE=3 SV=1)

HSP 1 Score: 193 bits (491), Expect = 6.56e-62
Identity = 98/112 (87.50%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNV+LAG D+AAIFSATTTAVSSIIGKPEN+VMVLL GSV ISFGGNG+PA F
Sbjct: 1   MPCLYISTNVNLAGVDSAAIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAVF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. ExPASy TrEMBL
Match: A0A0A0L1F7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314440 PE=3 SV=1)

HSP 1 Score: 193 bits (490), Expect = 9.31e-62
Identity = 98/112 (87.50%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNV+LAG D+A IFSATTTAVSSIIGKPEN+VMVLL GSV ISFGGNG+PAAF
Sbjct: 1   MPCLYISTNVNLAGVDSAPIFSATTTAVSSIIGKPENYVMVLLNGSVPISFGGNGDPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LIST+ +ILN+KLSVPPARFFLKVHDTTAGRPISKL
Sbjct: 61  AEVVSMGGINSEVKRRLISTLGSILNEKLSVPPARFFLKVHDTTAGRPISKL 112

[Close]

BLAST of MC00g0495 vs. ExPASy TrEMBL
Match: A0A6J1IKS8 (macrophage migration inhibitory factor homolog OS=Cucurbita maxima OX=3661 GN=LOC111478343 PE=3 SV=1)

HSP 1 Score: 192 bits (487), Expect = 2.67e-61
Identity = 98/112 (87.50%), Postives = 107/112 (95.54%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYISTNVDLAGAD+AAIF+ATT+AVSSIIGKPEN+VMVLLKGSVAISFGGN EPAAF
Sbjct: 1   MPCLYISTNVDLAGADSAAIFAATTSAVSSIIGKPENYVMVLLKGSVAISFGGNKEPAAF 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 112
           AEVVSMGGINS+VKR LI+TI +IL +KLSVPPARF LKVHDTTAGRP+SKL
Sbjct: 61  AEVVSMGGINSEVKRRLIATIGSILKEKLSVPPARFVLKVHDTTAGRPVSKL 112

[Close]

BLAST of MC00g0495 vs. TAIR 10
Match: AT5G01650.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 1.3e-28
Identity = 59/103 (57.28%), Postives = 82/103 (79.61%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCL +STNV+L G DT++I S  ++ V+ IIGKPEN+VM++LKGSV +SFGG  +PAA+
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMIVLKGSVPMSFGGTEDPAAY 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDT 104
            E+VS+GG+N+ V + L + ++AIL  KLSVP +RFFLK +DT
Sbjct: 61  GELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDT 103

[Close]

BLAST of MC00g0495 vs. TAIR 10
Match: AT5G01650.2 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 1.3e-28
Identity = 59/103 (57.28%), Postives = 82/103 (79.61%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCL +STNV+L G DT++I S  ++ V+ IIGKPEN+VM++LKGSV +SFGG  +PAA+
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGKPENYVMIVLKGSVPMSFGGTEDPAAY 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDT 104
            E+VS+GG+N+ V + L + ++AIL  KLSVP +RFFLK +DT
Sbjct: 61  GELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDT 103

[Close]

BLAST of MC00g0495 vs. TAIR 10
Match: AT3G51660.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 121.3 bits (303), Expect = 4.9e-28
Identity = 63/112 (56.25%), Postives = 80/112 (71.43%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MPCLYI+TNV+  G +T   +S  T AV+SI+G+P+N VMV+LKGSV I FGGN E AA+
Sbjct: 1   MPCLYITTNVNFDGVNTDPFYSEVTKAVASIVGRPQNLVMVVLKGSVEIVFGGNKEAAAY 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 113
           AE+VSMGGI  QVKR LI+T+ +IL+   S+ P RF  KV D  +    SKL
Sbjct: 61  AEIVSMGGITKQVKRELIATVGSILHTHFSIHPTRFIFKVFDINSLPLPSKL 112

[Close]

BLAST of MC00g0495 vs. TAIR 10
Match: AT5G57170.2 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 1.3e-17
Identity = 47/112 (41.96%), Postives = 69/112 (61.61%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MP L + TN+ +     + I    T AV+ IIGKPE++VM+LL   V I+F G  EPAA+
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAGTEEPAAY 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDTTAGRPISKL 113
            E++S+GG+   V   L  TI+ IL  KLS+  +RF++K +D+    P+S L
Sbjct: 61  GELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYDS----PVSPL 108

[Close]

BLAST of MC00g0495 vs. TAIR 10
Match: AT5G57170.1 (Tautomerase/MIF superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 3.0e-17
Identity = 44/103 (42.72%), Postives = 65/103 (63.11%), Query Frame = 0


Query: 1   MPCLYISTNVDLAGADTAAIFSATTTAVSSIIGKPENFVMVLLKGSVAISFGGNGEPAAF 60
           MP L + TN+ +     + I    T AV+ IIGKPE++VM+LL   V I+F G  EPAA+
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAGTEEPAAY 60

Query: 61  AEVVSMGGINSQVKRNLISTIAAILNDKLSVPPARFFLKVHDT 104
            E++S+GG+   V   L  TI+ IL  KLS+  +RF++K +D+
Sbjct: 61  GELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYDS 103

The following BLAST results are available for this feature:

BLAST of MC00g0495 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P81748	9.3e-08	30.39	Macrophage migration inhibitory factor homolog OS=Trichuris trichiura OX=36087 P...	[more]
P81529	1.3e-06	31.63	Macrophage migration inhibitory factor homolog OS=Trichinella spiralis OX=6334 P...	[more]
P91850	6.7e-06	27.62	Macrophage migration inhibitory factor homolog OS=Brugia malayi OX=6279 GN=Bm1_2...	[more]
P80177	4.3e-05	26.67	Macrophage migration inhibitory factor OS=Bos taurus OX=9913 GN=MIF PE=1 SV=6	[more]
Q1ZZU7	4.3e-05	26.67	Macrophage migration inhibitory factor OS=Ovis aries OX=9940 GN=MIF PE=3 SV=1	[more]

BLAST of MC00g0495 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022144090.1	5.53e-70	100.00	macrophage migration inhibitory factor homolog [Momordica charantia]	[more]
XP_038883562.1	2.00e-63	90.18	macrophage migration inhibitory factor homolog [Benincasa hispida]	[more]
XP_022949815.1	4.72e-62	88.39	macrophage migration inhibitory factor homolog [Cucurbita moschata] >XP_02354310...	[more]
XP_008464238.1	1.35e-61	87.50	PREDICTED: macrophage migration inhibitory factor homolog [Cucumis melo]	[more]
XP_004148599.1	1.92e-61	87.50	macrophage migration inhibitory factor homolog [Cucumis sativus]	[more]

BLAST of MC00g0495 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CQN7	2.68e-70	100.00	macrophage migration inhibitory factor homolog OS=Momordica charantia OX=3673 GN...	[more]
A0A6J1GDU8	2.29e-62	88.39	macrophage migration inhibitory factor homolog OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A1S3CL09	6.56e-62	87.50	macrophage migration inhibitory factor homolog OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0L1F7	9.31e-62	87.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G314440 PE=3 SV=1	[more]
A0A6J1IKS8	2.67e-61	87.50	macrophage migration inhibitory factor homolog OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of MC00g0495 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01650.1	1.3e-28	57.28	Tautomerase/MIF superfamily protein	[more]
AT5G01650.2	1.3e-28	57.28	Tautomerase/MIF superfamily protein	[more]
AT3G51660.1	4.9e-28	56.25	Tautomerase/MIF superfamily protein	[more]
AT5G57170.2	1.3e-17	41.96	Tautomerase/MIF superfamily protein	[more]
AT5G57170.1	3.0e-17	42.72	Tautomerase/MIF superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014347	Tautomerase/MIF superfamily	GENE3D	3.30.429.10	Macrophage Migration Inhibitory Factor	coord: 1..107 e-value: 1.2E-30 score: 108.3
IPR014347	Tautomerase/MIF superfamily	SUPERFAMILY	55331	Tautomerase/MIF	coord: 1..104
IPR001398	Macrophage migration inhibitory factor	PFAM	PF01187	MIF	coord: 2..104 e-value: 7.8E-12 score: 45.5
IPR001398	Macrophage migration inhibitory factor	PANTHER	PTHR11954	D-DOPACHROME DECARBOXYLASE	coord: 1..112
None	No IPR available	PANTHER	PTHR11954:SF25	LS1-LIKE PROTEIN	coord: 1..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0495.1	MC00g0495.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005615	extracellular space
molecular_function	GO:0050178	phenylpyruvate tautomerase activity