MC00g0477 (gene) Bitter gourd (Dali-11) v1

Overview
NameMC00g0477
Typegene
OrganismMomordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Descriptionproactivator polypeptide-like 1
Locationscaffold196: 251264 .. 255439 (-)
RNA-Seq ExpressionMC00g0477
SyntenyMC00g0477
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCGAATTGCACGTGAGAAATATCCGCATGAGAAAAAATAAAAATAATAAAAAAAAAATTCTTAAAGAGCGATATTGAAAATTGGAATGAATGTTCCAATTCCAAGTTTCTAGAGGGAATCAGTTCCATCGTCTCTTTCGGACGCCTCGGAACGAAACCAGAGCCTGGTTGTCTAAGCGAAGGCATGTATTTCTCTGCAATCTGATTTCTGTTCTCCTTGAAATCCGAAATTCTTCATCCCGCGCTCTGAGAAACCGCCGGGAAGAAGATCTATAGATCATCCGCGTTTGCGGTTAAATTTTCTGGGAATCTAGATCTTTTTCCTTCGTTTTCTTTGCTGCCAAACGCATTTTCTAGGTTTTTTTTCTTCCTAATTTTATGATTGGGGCGCATGGCTTTTATAACCCTCGGTCTTTTGTTCTATTTGTATTTTGTCCTTATTTCATTGACGTCTGTATGGAAATATCTTTGTTTCGTCAGTAAGTACGTGGTGTGGTAATTTCAGTTGAAGCTTACTTTTTATTGCTGTTTTTCGTCGTATTCTCAGGCATCAGGCGCAATGGATTTGAGGTTTGGACTAATTTTCCTTCTCGTGGTTGGTGCTGCTTGGGACTGTGATGCTAGAAATCTTGCATCCTCTGGTAAATCTTTATTTTTCATTTTTGATCGATATGTCTGATTGTTGTTTAGAATTGGATAAATATGTAACGTAATCTAGGCCGTAATCTCAATTTCTTCTCTAATTTTTTGGGAAATTCAAATTTATCTTTTTTGTGTATTGATTTTTCCCCCATGAAATTTAGATTGAAACGAATTTAAATAGAGTATTTTGGTCATGAGGTTTTATGAGTCTAATTTCCACAAGCCATTTCAAACCGAATAAGTTTCTGAATTTATGGATGAGCTTGTCTATTTCATCCCATAAAAAGGTAAAGTATGGGTATTTGTTAATTAATAAAATGAATTTTGATGCTACATCGTCATTTTGTCAATGAATTTTCACATTTTGACGGCAGTAAGAATTCGATTGAAAATAAATTAATTCATTAAAGATGAAATTGGGATTTTTAAAAATAGTCCAAAAGGGAGTAAACTATAACTTAGTCTTTAGAATTTGATGGTTCTGGTTATGACTTATGTCTCCTGATTTTTTGTGAGCTCTGTTTTATATTATTTGGCTCATTAAAATGACTAATTGTATTTCTTCTTGTTCTGTTTTTTTCTTTTGGTTAATGATTGTTTAACTTGAGATCAAATGCACAGACGTCTCCTCGTATTACTTGTCTTTAAGACTTCCAACAAACATAAGCATTATGTGAAGATGACATTCTAATTGAAAAACCTTTAAATCTTTTGCTTTGAGTGAGACTTTTATCTCATCAAAAAAGTCGTCATTTCTGCTGTTTCAGATTCTGAGTTAAGCTACCTAAGGCAAGAGAAGGACAATGAGGCTTTATCTGAAGCTTCCGAGGATCCGAAGATATGTAAACTCTGTGAGAGCTTGGTCAGTCAGACAGTTGAATACCTTGCAGATAACAAGACCCAGGATGAGATCACTGTTATTCTCCGGAAAACCTGCGCTGTGTTGGGCCTATTCAATGAGAAGGTATGGCTGCTTACGCTTATACGACTTTTTATTTCACGAATCTGCAGGAGTGTGCATTTGAAATGATTGATAATTTTCTGTTCATTTATAACGAAGAAAAACTTTTTTCGGCTAGCCTAGCTATAGCATAGTATCTTCCATCATTTATCATCTCACTCTCAAATGAAGTGACAGAACAAAAGAATTTACTCTCGTTGATATTATCTTTGTTCCATTTTTTCAGTGCATCAGTCTGGTGGACTACTACGCTCCTCTCTTCTTCTCAGAGATTTCCTCAATTGAACCTACCAGCTTCTGCCAGTCAGCCCGATTCTGTGAGCAAATTACCGTAATGTCCTTGCAGATTCAGGAAAATAGCTGTGGATTTTGTCATCAGACTGTTTCAAAAATGGTGGAGAAGTTGAAAGATCCTAACACACAGGTTTGATATTATACATTCTGCTGTTTTATTTGCTTATGAGGAAATTAAAAAACTTTTTAGTGATCTATGAAAATTTTAACACTCTTGGTCTGGATTTCTTATTGGATTAAATTGTGCGGATTGAGTGGGGATGCAGAGTTCTGAACAAACACCTTTTCTTTTGTCAGAATGACTTTGTTGTGAACATTTGTTGATTGGAACGTAGCAGTAATATATATGTATTTATGACTGTGTTTAAACTTATCACAAAGTCTATAGTTGTTAAGAAATATATAAACATCTTCAGGATGGTAGTTTCTTATATATTTTTTCGGGTTTGTGAACATCTCTTATACGCTTTCTGTGTTGCAGATAGAAATACTTGAGACGCTTCTGAATATGTGCAACTCTTTGGGGTACCGTGTGAAAGAGGTATGAAGAATTTTCACTAACATTTATGCGTCCACTTATTTTTTCTGGTTTTGCTGTTTGAGAATGGTGAAAAGAATACGTAATTCATGTCCCTATTTCTGTCCTTTATTTTTATCCATTTCAGTTCCAGAAGTTATAAAAAAAAAATATTTGGTCTTTCTTTCACGTTTATATAATATCCAAACGTAACCAATAAGAGGTTGACCAAATTATCTAAACTTCCCATATCTAGTCTTCGATTGCCTCTTTTCAGCGGTCAGCCAACTTCTAATTTTGTTTCTTCCGTTGGTTTATTTGAGGGCGTTCCATAATCATTTCATTTTTCGTTTTTAGAAATTATGTTTGTTTTGTATATCTCATTAGTAAATTTTTCGCGAGTCTTGAAAACATATTTGAATTCTTAGCCAAATTTCAAAAATAAAAATAAATTTTTGAAATTGTTTTCAAAATTTGACTTAGATTTTGAAAATGTTTATAAAAAATTATATAACCAAGTAAAGGAAAAGTTTAATTTTTAAAAATAGAAAATAAAAAATGGAATAGTTATCCAGTTTTATTAATTCAATTTTAATTTTATAACGGAGTGTTTGAGGTCCACACTCCGTACATCATTAATTTCATGACTAGTGATTTGAAGCTGAGTTGCCCACAATTTTGCTTCCAATATATTCCTAAAATTTGTTACTTTTTCTCCGCAAGCTCAAATTTTTTAAACTGCGCATTATCATATTATATATAGTTTTTCAAGGTGCAAAATGTGTGTGTGTATATATATATATTTGTTGTGCACCTAAAAACACACACTCTGATGCTTAAGTCGTAGAGGACTATCCCACTATAGTGGGCCTCACACTTTAAGAAAATATTGTTCATGAATACCTTCACTAATGTTCAGGGTCTTGCTCACCCAACTAAGCTTTGAGATTTAATAGAAGTTTTAAAGTATTAAGTTTTTTATTTGAAGGTTTGAACTCAGGACTTTAGATATTATTTCTAAATATACGGAGACTTCATTATCAGCTTTCCGGTACCTTTATGGTTAGTATTAGTAGACACTTATTTCAACTCAAAATCTTGCTCAATCATTATAATTATCTCTTGCATTTATAATACTATCACACAATACCACACAACCAACTAACGATCAATATGTTATTATTATTATTATTATTTTTTGTCCATTAAGCCAAGCCAAAAATAAAGCAATCTAATATAGGCAATCAATCTCTACTTTTTCATATTGTGATATTTTCAATTATTGAGCAGTGCAAGAGACTGGTATTTGAATATGGGCCTCTGATTGTGGCCAAGTCGGAGAAAATTCTAGAAAATACAGATATTTGCAGAGCAATACATGCTTGCCCGGCGGTAGCGGGTGGCGACAACACCACATCATTTGTTGGAACTCTGCCTGCGCTTGCCGACGCTTGAATTTCCACACCAGACACGATGGGATCGACATGTCGTTTCTGAAGGAAAATGGGTAAGAATTTAGTTGTTCTCTTGCCCTTCTCTGTCTGTATAAGAATCTTAATATTATACAATCTCTGTCCATGGATGGTCTGGGTATAATGATGTACGTTCTGACACAAGATTATATGTATATGATTGGCTGTTGCTCTATTCAGTGTATAATTTATAAACGTGTCACTGTTGGTGGAACTTTGGAATTGTCCAGATGTGGCCTAAGCATGTACTCTTCAATTTAGGTAGCGTTTATACGCGTCGGACACTCTG

mRNA sequence

GTTCGAATTGCACGTGAGAAATATCCGCATGAGAAAAAATAAAAATAATAAAAAAAAAATTCTTAAAGAGCGATATTGAAAATTGGAATGAATGTTCCAATTCCAAGTTTCTAGAGGGAATCAGTTCCATCGTCTCTTTCGGACGCCTCGGAACGAAACCAGAGCCTGGTTGTCTAAGCGAAGGCATGTATTTCTCTGCAATCTGATTTCTGTTCTCCTTGAAATCCGAAATTCTTCATCCCGCGCTCTGAGAAACCGCCGGGAAGAAGATCTATAGATCATCCGCGTTTGCGGTTAAATTTTCTGGGAATCTAGATCTTTTTCCTTCGTTTTCTTTGCTGCCAAACGCATTTTCTAGGTTTTTTTTCTTCCTAATTTTATGATTGGGGCGCATGGCTTTTATAACCCTCGGTCTTTTGTTCTATTTGTATTTTGTCCTTATTTCATTGACGTCTGTATGGAAATATCTTTGTTTCGTCAGTAAGTACGTGGTGTGGTAATTTCAGTTGAAGCTTACTTTTTATTGCTGTTTTTCGTCGTATTCTCAGGCATCAGGCGCAATGGATTTGAGGTTTGGACTAATTTTCCTTCTCGTGGTTGGTGCTGCTTGGGACTGTGATGCTAGAAATCTTGCATCCTCTGATTCTGAGTTAAGCTACCTAAGGCAAGAGAAGGACAATGAGGCTTTATCTGAAGCTTCCGAGGATCCGAAGATATGTAAACTCTGTGAGAGCTTGGTCAGTCAGACAGTTGAATACCTTGCAGATAACAAGACCCAGGATGAGATCACTGTTATTCTCCGGAAAACCTGCGCTGTGTTGGGCCTATTCAATGAGAAGTGCATCAGTCTGGTGGACTACTACGCTCCTCTCTTCTTCTCAGAGATTTCCTCAATTGAACCTACCAGCTTCTGCCAGTCAGCCCGATTCTGTGAGCAAATTACCGTAATGTCCTTGCAGATTCAGGAAAATAGCTGTGGATTTTGTCATCAGACTGTTTCAAAAATGGTGGAGAAGTTGAAAGATCCTAACACACAGATAGAAATACTTGAGACGCTTCTGAATATGTGCAACTCTTTGGGGTACCGTGTGAAAGAGTGCAAGAGACTGGTATTTGAATATGGGCCTCTGATTGTGGCCAAGTCGGAGAAAATTCTAGAAAATACAGATATTTGCAGAGCAATACATGCTTGCCCGGCGGTAGCGGGTGGCGACAACACCACATCATTTGTTGGAACTCTGCCTGCGCTTGCCGACGCTTGAATTTCCACACCAGACACGATGGGATCGACATGTCGTTTCTGAAGGAAAATGGGTAAGAATTTAGTTGTTCTCTTGCCCTTCTCTGTCTGTATAAGAATCTTAATATTATACAATCTCTGTCCATGGATGGTCTGGGTATAATGATGTACGTTCTGACACAAGATTATATGTATATGATTGGCTGTTGCTCTATTCAGTGTATAATTTATAAACGTGTCACTGTTGGTGGAACTTTGGAATTGTCCAGATGTGGCCTAAGCATGTACTCTTCAATTTAGGTAGCGTTTATACGCGTCGGACACTCTG

Coding sequence (CDS)

ATGGATTTGAGGTTTGGACTAATTTTCCTTCTCGTGGTTGGTGCTGCTTGGGACTGTGATGCTAGAAATCTTGCATCCTCTGATTCTGAGTTAAGCTACCTAAGGCAAGAGAAGGACAATGAGGCTTTATCTGAAGCTTCCGAGGATCCGAAGATATGTAAACTCTGTGAGAGCTTGGTCAGTCAGACAGTTGAATACCTTGCAGATAACAAGACCCAGGATGAGATCACTGTTATTCTCCGGAAAACCTGCGCTGTGTTGGGCCTATTCAATGAGAAGTGCATCAGTCTGGTGGACTACTACGCTCCTCTCTTCTTCTCAGAGATTTCCTCAATTGAACCTACCAGCTTCTGCCAGTCAGCCCGATTCTGTGAGCAAATTACCGTAATGTCCTTGCAGATTCAGGAAAATAGCTGTGGATTTTGTCATCAGACTGTTTCAAAAATGGTGGAGAAGTTGAAAGATCCTAACACACAGATAGAAATACTTGAGACGCTTCTGAATATGTGCAACTCTTTGGGGTACCGTGTGAAAGAGTGCAAGAGACTGGTATTTGAATATGGGCCTCTGATTGTGGCCAAGTCGGAGAAAATTCTAGAAAATACAGATATTTGCAGAGCAATACATGCTTGCCCGGCGGTAGCGGGTGGCGACAACACCACATCATTTGTTGGAACTCTGCCTGCGCTTGCCGACGCTTGA

Protein sequence

MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLVSQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQSARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKECKRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA
Homology
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BLAST of MC00g0477 vs. ExPASy Swiss-Prot
Match: Q8C1C1 (Proactivator polypeptide-like 1 OS=Mus musculus OX=10090 GN=Psapl1 PE=1 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 7.6e-12
Identity = 46/176 (26.14%), Postives = 82/176 (46.59%), Query Frame = 0 

Query: 53  CKLCESLVSQTVEYLADNKTQDEITVILRKTCAVL-GLFNEKCISLVDYYAPLFFSEISS 112
           C +C +LV +  ++L  N T+  I+  L + C V+     ++CI+LVD Y+P     +S 
Sbjct: 295 CDVCLNLVQELDKWLVTNSTEALISHTLERVCTVVPEPLVQQCITLVDTYSPELVQLMSK 354

Query: 113 IEPTSFCQSARFCEQ-----------ITVMSLQIQENS----CGFCHQTVSKMVEKLKDP 172
           + P   C++ + C              T  SL + E +    C  C + +    + L   
Sbjct: 355 VTPEKVCETIKLCGSKRRARSISRAVATTPSLPVDEENQGSFCQGCKRLLGMSSQNLDHK 414

Query: 173 NTQIEILETLLNMCNSLGY-RVKECKRLVFEYGPLIVAKSEKILENTDICRAIHAC 212
           +T+ +IL      C  L    V +C R V EY P+++   + ++  TD+C+ + AC
Sbjct: 415 STKRDILNAFKGGCRILPLPYVMQCNRFVAEYEPVLIESLKFMMNPTDLCKKMGAC 470

[Close]
BLAST of MC00g0477 vs. ExPASy Swiss-Prot
Match: P26779 (Prosaposin OS=Bos taurus OX=9913 GN=PSAP PE=1 SV=3)

HSP 1 Score: 67.0 bits (162), Expect = 3.2e-10
Identity = 47/171 (27.49%), Postives = 82/171 (47.95%), Query Frame = 0 

Query: 53  CKLCESLVSQTVEYLADNKTQDEITVILRKTCAVLGL-FNEKCISLVDYYAPLFFS-EIS 112
           C++CE +V +  + + +N+T++EI   L K C+ L     E+C  +VD Y     S  + 
Sbjct: 316 CEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGSSILSILLD 375

Query: 113 SIEPTSFCQSARFCEQ-----ITVMSLQIQENS-CGFCHQTVSKMVEKLKDPNTQIEILE 172
              P   C     C        TV  +  ++   C  C + V  +   L+  +T+ +IL 
Sbjct: 376 EASPELVCSMLHLCSSRGLPAATVRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILA 435

Query: 173 TLLNMCNSL--GYRVKECKRLVFEYGPLIVAKSEKILENTDICRAIHACPA 214
            L   C+ L   YR K+C + V EY P+++    ++++ + +C  I ACPA
Sbjct: 436 ALEKGCSFLPDQYR-KQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPA 485

[Close]
BLAST of MC00g0477 vs. NCBI nr
Match: XP_022143993.1 (proactivator polypeptide-like 1 [Momordica charantia] >XP_022143994.1 proactivator polypeptide-like 1 [Momordica charantia])

HSP 1 Score: 468 bits (1203), Expect = 3.38e-166
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV
Sbjct: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS
Sbjct: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC
Sbjct: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA
Sbjct: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233

[Close]
BLAST of MC00g0477 vs. NCBI nr
Match: XP_022925048.1 (proactivator polypeptide-like 1 [Cucurbita moschata])

HSP 1 Score: 375 bits (964), Expect = 9.02e-130
Identity = 182/233 (78.11%), Postives = 209/233 (89.70%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EIT IL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+  LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSLLADA 233

[Close]
BLAST of MC00g0477 vs. NCBI nr
Match: KAG7027542.1 (Proactivator polypeptide-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 376 bits (966), Expect = 1.44e-129
Identity = 182/233 (78.11%), Postives = 210/233 (90.13%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 34  MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 93

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EIT IL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 94  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 153

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 154 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 213

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 214 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 266

[Close]
BLAST of MC00g0477 vs. NCBI nr
Match: XP_022966507.1 (proactivator polypeptide-like 1 [Cucurbita maxima])

HSP 1 Score: 375 bits (962), Expect = 1.82e-129
Identity = 180/233 (77.25%), Postives = 210/233 (90.13%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGL+FLLVVGAAW+CDAR LASSD   SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVGAAWNCDARKLASSDHVSSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EIT IL++TCAVLG+F E+C+SLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECVSLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 233

[Close]
BLAST of MC00g0477 vs. NCBI nr
Match: XP_023518446.1 (proactivator polypeptide-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372 bits (956), Expect = 1.49e-128
Identity = 179/231 (77.49%), Postives = 208/231 (90.04%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EIT IL++TCAVLG+F E+C+SLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECVSLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALA 231
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLA 231

[Close]
BLAST of MC00g0477 vs. ExPASy TrEMBL
Match: A0A6J1CS32 (proactivator polypeptide-like 1 OS=Momordica charantia OX=3673 GN=LOC111013781 PE=4 SV=1)

HSP 1 Score: 468 bits (1203), Expect = 1.64e-166
Identity = 233/233 (100.00%), Postives = 233/233 (100.00%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV
Sbjct: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS
Sbjct: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC
Sbjct: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA
Sbjct: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233

[Close]
BLAST of MC00g0477 vs. ExPASy TrEMBL
Match: A0A6J1EE54 (proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111432411 PE=4 SV=1)

HSP 1 Score: 375 bits (964), Expect = 4.36e-130
Identity = 182/233 (78.11%), Postives = 209/233 (89.70%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGL+FLLVV AAWDCDAR LASSD E SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVCAAWDCDARKLASSDHESSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EIT IL++TCAVLG+F E+CISLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECISLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+  LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSLLADA 233

[Close]
BLAST of MC00g0477 vs. ExPASy TrEMBL
Match: A0A6J1HN59 (proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466139 PE=4 SV=1)

HSP 1 Score: 375 bits (962), Expect = 8.80e-130
Identity = 180/233 (77.25%), Postives = 210/233 (90.13%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFGL+FLLVVGAAW+CDAR LASSD   SYL  EKD EA SEAS +PKICKLCESLV
Sbjct: 1   MDLRFGLVFLLVVGAAWNCDARKLASSDHVSSYLELEKDVEASSEASSNPKICKLCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLADN TQ+EIT IL++TCAVLG+F E+C+SLVD Y PLFFSEISSIEP+S CQS
Sbjct: 61  SQAVEYLADNNTQNEITGILQQTCAVLGVFKEECVSLVDNYVPLFFSEISSIEPSSICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
           AR CEQ+T++S QIQ+NSCGFC +T+SK+++KLKDP+TQIEIL+TLLNMC+SLGYRVK+C
Sbjct: 121 ARICEQVTIISSQIQDNSCGFCQETISKILDKLKDPDTQIEILQTLLNMCDSLGYRVKQC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEK+LE T+IC+AIHACPAVAGGDNTTS VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKVLEQTNICKAIHACPAVAGGDNTTSSVGTVSSLADA 233

[Close]
BLAST of MC00g0477 vs. ExPASy TrEMBL
Match: A0A6J1GCQ1 (proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111452990 PE=4 SV=1)

HSP 1 Score: 362 bits (928), Expect = 1.33e-124
Identity = 173/233 (74.25%), Postives = 202/233 (86.70%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFG++FLLVVG AWDCDARNLAS DSELSYL+Q KD EALSEAS  PK+C+LCESLV
Sbjct: 1   MDLRFGIVFLLVVGVAWDCDARNLASFDSELSYLKQGKDVEALSEASSKPKVCELCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLA+N+TQ EI  ILR+TCAV+GLF E+C+SLVD Y PLFFSE SSIEP S CQS
Sbjct: 61  SQAVEYLAENQTQSEIIRILRQTCAVVGLFKEECVSLVDSYVPLFFSEASSIEPASICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
            RFCEQ+TV+S QIQE+SC FCHQT+SK+++KLKDP+TQ+EIL+ LLN+C+SLG R KEC
Sbjct: 121 VRFCEQVTVISSQIQEHSCEFCHQTISKILDKLKDPDTQVEILQALLNICDSLGKRAKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEKILE TDIC+AIHAC  +AGGD   S VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKILEQTDICKAIHACTGIAGGDKAISSVGTVSSLADA 233

[Close]
BLAST of MC00g0477 vs. ExPASy TrEMBL
Match: A0A6J1IPM0 (proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111478309 PE=4 SV=1)

HSP 1 Score: 361 bits (926), Expect = 2.68e-124
Identity = 172/233 (73.82%), Postives = 202/233 (86.70%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           MDLRFG++F LVVGAAWDCDARNLAS DSELSYL+Q KD EALSEAS  PK+C+LCESLV
Sbjct: 1   MDLRFGIVFFLVVGAAWDCDARNLASFDSELSYLKQGKDVEALSEASSKPKVCELCESLV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           SQ VEYLA+N+TQ EI  ILR+TCAV+GLF E+C+SLVD Y PLFFSE SSIEP S CQS
Sbjct: 61  SQAVEYLAENQTQSEIIRILRQTCAVVGLFKEECVSLVDSYVPLFFSEASSIEPASICQS 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
            RFCEQ+TV+S QIQE+SC FCHQT+SK+++KLKDP+TQ+EIL+ LLN+C+SLG R KEC
Sbjct: 121 VRFCEQVTVISSQIQEHSCEFCHQTISKILDKLKDPDTQVEILQALLNICDSLGKRAKEC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 233
           K+LVFEYGPLI+A SEKILE TDIC+AIH+CP +AG D   S VGT+ +LADA
Sbjct: 181 KKLVFEYGPLILANSEKILEQTDICKAIHSCPGIAGDDKAISSVGTVSSLADA 233

[Close]
BLAST of MC00g0477 vs. TAIR 10
Match: AT3G51730.1 (saposin B domain-containing protein )

HSP 1 Score: 164.5 bits (415), Expect = 1.0e-40
Identity = 84/212 (39.62%), Postives = 125/212 (58.96%), Query Frame = 0 

Query: 1   MDLRFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLV 60
           M L+ G   LL++G     DAR+    DS +S            + S    +C LCE  V
Sbjct: 1   MGLKAGTFVLLLLGLILVSDARSFV--DSTIS-----------EKVSNKEDVCTLCEEYV 60

Query: 61  SQTVEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQS 120
           +  + YL  N TQ EI   L   C+ L  ++++CISLVDYY PLFF ++ S +P  FC+ 
Sbjct: 61  TDALSYLEKNVTQAEIIEDLHDRCSQLRGYSQQCISLVDYYVPLFFLQLESFQPHYFCKR 120

Query: 121 ARFCEQITVMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKEC 180
              C ++  +  + +++SCG CH+TVS+++ KL+DP+TQ++I+E L+  C SL    K+C
Sbjct: 121 MNLCGKVVALVEEARQDSCGVCHRTVSEILIKLQDPDTQLDIVELLIKGCKSLKNYEKKC 180

Query: 181 KRLVFEYGPLIVAKSEKILENTDICRAIHACP 213
           K LVFEYGPLI+  +E+ L   D+C  + ACP
Sbjct: 181 KTLVFEYGPLILVNAEEFLVKNDVCTLLRACP 199

[Close]
BLAST of MC00g0477 vs. TAIR 10
Match: AT5G01800.1 (saposin B domain-containing protein )

HSP 1 Score: 152.5 bits (384), Expect = 4.1e-37
Identity = 76/231 (32.90%), Postives = 130/231 (56.28%), Query Frame = 0 

Query: 4   RFGLIFLLVVGAAWDCDARNLASSDSELSYLRQEKDNEALSEASEDPKICKLCESLVSQT 63
           RFG++ +L +  +W C A N                 E    A +D ++C+LC+  V+  
Sbjct: 4   RFGVLLVLFL-LSWSCHATNPILL-------------EPFESAHDDNQVCELCDKYVTLV 63

Query: 64  VEYLADNKTQDEITVILRKTCAVLGLFNEKCISLVDYYAPLFFSEISSIEPTSFCQSARF 123
           ++YL D   Q+E+   L  +C+ +    ++C+S+VD+Y  LFF+++S+I+    C+    
Sbjct: 64  IDYLQDYDNQNELVEALHISCSQIPPLKKQCLSMVDHYTQLFFTQVSTIKSDQICKRLNL 123

Query: 124 CEQIT-VMSLQIQENSCGFCHQTVSKMVEKLKDPNTQIEILETLLNMCNSLGYRVKECKR 183
           C+ +T   + Q+ + +C  C +TVS++V KLKDP T+++I+  LL  C SL     +CK+
Sbjct: 124 CQAVTPAFASQVHQGNCEACRETVSEVVTKLKDPETKLKIIRLLLKECKSLNNYQDKCKK 183

Query: 184 LVFEYGPLIVAKSEKILENTDICRAIHACPAVAGGDNTTSFVGTLPALADA 234
           +VFEYGPL++   +K LE  D+C  +H CP   G      +V  + +LAD+
Sbjct: 184 MVFEYGPLMLTDLQKFLEKKDVCTILHVCP---GPATHRDYVPAVESLADS 217

The following BLAST results are available for this feature:
BLAST of MC00g0477 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8C1C17.6e-1226.14Proactivator polypeptide-like 1 OS=Mus musculus OX=10090 GN=Psapl1 PE=1 SV=2[more]
P267793.2e-1027.49Prosaposin OS=Bos taurus OX=9913 GN=PSAP PE=1 SV=3[more]
BLAST of MC00g0477 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022143993.13.38e-166100.00proactivator polypeptide-like 1 [Momordica charantia] >XP_022143994.1 proactivat... [more]
XP_022925048.19.02e-13078.11proactivator polypeptide-like 1 [Cucurbita moschata][more]
KAG7027542.11.44e-12978.11Proactivator polypeptide-like 1, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_022966507.11.82e-12977.25proactivator polypeptide-like 1 [Cucurbita maxima][more]
XP_023518446.11.49e-12877.49proactivator polypeptide-like 1 [Cucurbita pepo subsp. pepo][more]
BLAST of MC00g0477 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1CS321.64e-166100.00proactivator polypeptide-like 1 OS=Momordica charantia OX=3673 GN=LOC111013781 P... [more]
A0A6J1EE544.36e-13078.11proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111432411 PE... [more]
A0A6J1HN598.80e-13077.25proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466139 PE=4... [more]
A0A6J1GCQ11.33e-12474.25proactivator polypeptide-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111452990 PE... [more]
A0A6J1IPM02.68e-12473.82proactivator polypeptide-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111478309 PE=4... [more]
BLAST of MC00g0477 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G51730.11.0e-4039.62saposin B domain-containing protein [more]
AT5G01800.14.1e-3732.90saposin B domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008373SaposinPRINTSPR01797SAPOSINcoord: 53..73
score: 27.21
coord: 189..212
score: 26.79
coord: 136..158
score: 21.74
IPR008139Saposin B type domainSMARTSM00741sapb_4coord: 51..124
e-value: 1.5E-16
score: 71.0
coord: 137..211
e-value: 7.7E-13
score: 58.7
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 135..215
score: 12.668799
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 49..128
score: 14.899599
NoneNo IPR availableGENE3D1.10.225.10coord: 49..125
e-value: 1.5E-14
score: 56.3
NoneNo IPR availableGENE3D1.10.225.10coord: 134..215
e-value: 1.0E-16
score: 63.1
NoneNo IPR availablePANTHERPTHR11480SAPOSIN-RELATEDcoord: 1..212
NoneNo IPR availablePANTHERPTHR11480:SF80SAPOSIN-RELATEDcoord: 1..212
IPR008138Saposin B type, region 2PFAMPF03489SapB_2coord: 93..124
e-value: 4.0E-5
score: 23.7
coord: 178..211
e-value: 4.5E-7
score: 30.0
IPR007856Saposin-like type B, region 1PFAMPF05184SapB_1coord: 52..88
e-value: 4.2E-7
score: 29.9
coord: 139..174
e-value: 2.8E-6
score: 27.3
IPR011001Saposin-likeSUPERFAMILY47862Saposincoord: 51..126
IPR011001Saposin-likeSUPERFAMILY47862Saposincoord: 137..212

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MC00g0477.1MC00g0477.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0006665 sphingolipid metabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005615 extracellular space
cellular_component GO:0005764 lysosome
molecular_function GO:0004190 aspartic-type endopeptidase activity