MC00g0469 (gene) Bitter gourd (Dali-11) v1

BLAST of MC00g0469 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 8.7e-198
Identity = 336/574 (58.54%), Postives = 424/574 (73.87%), Query Frame = 0

Query: 10  TAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVLLNVSWDIKN 69
           T  NA+QALGRGFDV  D RLLYCKG  GS +V +++   RD+ L    +L NV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  FQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMD 129
            +   G +   +CSF EM   FN + G+      G FN+ F + GS Q+DAA+TK+L++ 
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G++IPL   +  K  LVL   ++RA+P+SWDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNNQGVYPQPTS 249
           +QHQSSPL   E++ YV D+   RF + +  + +G +K K+K                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 259

Query: 250 APYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALLDGVTGKEHL 309
                   D+TVIFRRRGGDDLEQ+H RWA TV  +PD+I MTF PI +LL+GV G  HL
Sbjct: 260 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 319

Query: 310 TRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQFSIMGQKLYV 369
           TRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQFS+MG KL++
Sbjct: 320 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFI 379

Query: 370 SIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGIGAPKWQGPE 429
           S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKILQP+WD+HV IGAPKWQGPE
Sbjct: 380 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 439

Query: 430 EQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGPRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFG +NVL++KLL
Sbjct: 440 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 499

Query: 490 YSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAKLVDTSEMSK 549
           +S++PGCTIRR++WDH     S +L     +++   SS+   + Q  KLAK+VD+SEM K
Sbjct: 500 FSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTS---SSTEEVSGQSGKLAKIVDSSEMLK 559

Query: 550 GPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584
           GP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 560 GPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of MC00g0469 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 7.5e-117
Identity = 251/599 (41.90%), Postives = 359/599 (59.93%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVS-GSTIVEV-DDEHTRDICLYDDVVLLNVSWDIKNFQ 73
           A+ ++G G+D+  D RL YCKG S  S ++++ + +   +I L   + + NVS  IK  +
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 74  EPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMDGF 133
                  S I  F +M   FNQ+  L+    SG FN+ F F+   Q DAA TKNL+ DG 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 134 YIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYVKQ 193
           +I L      K+ ++L+E+VK+A+P++WDP +LA FI+ +GTH+I S+ +GGKDVIY KQ
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 194 HQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGS--------IKLKEKGLDGGLFNNQGV 253
             SS L   ++++ ++++A++RF +   +  +GS        ++ KE+ L     ++ G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 254 YPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRLSPDVIEMTFFPITALLDG 313
           Y           KED   + +RRGG+D     H  W +TV++ PDVI M+F PIT+LL+G
Sbjct: 254 Y---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 313

Query: 314 VTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVP-GHQRKEPVCPSLQFS 373
           V G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C SLQFS
Sbjct: 314 VPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFS 373

Query: 374 IMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGIG 433
             G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI Q   D +  I 
Sbjct: 374 FFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI- 433

Query: 434 APKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGPR 493
                  E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V   G +
Sbjct: 434 -----RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFK 493

Query: 494 NVLYMKLLYSRLPGCT-IRRTLWDHLLNDKSKK-----LVS-------------SSDNSN 553
           NVL+++L +SR+ G T ++ + WD  +    K      L+S             +  N N
Sbjct: 494 NVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNIN 553

Query: 554 ADDSSSGSR-NIQGNKLAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSL 581
           +     G     Q  KL K VDTSEM++GP + PG+W+V+GA+L VEKGKI L+VKYSL
Sbjct: 554 SAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of MC00g0469 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 9.5e-104
Identity = 237/603 (39.30%), Postives = 332/603 (55.06%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGV-SGSTIVEVDDEHTRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMD 130
            +       S I SF++M   FNQ   LS    SG FN+ F F+     DA++ K L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYV 190
           G++I L   +  +  L L++ VKR +P+SWD  +LA FIE +GTHV+  +T+GGKDVI+V
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFS----DTQGLTGSGSIKLKEKGLDGGLFNNQGVYP 250
           KQ + S     E+++ ++   ++RF     +++      S K KE+ L        G+ P
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENL-----LQWGLQP 254

Query: 251 QPTSAPYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALLD 310
             TS        T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL 
Sbjct: 255 FGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLS 314

Query: 311 GVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVP-GHQRKEPVCPSLQF 370
           G+ G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQF
Sbjct: 315 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 374

Query: 371 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQ-PYWDTHVG 430
           S+MG KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  L   + DT+  
Sbjct: 375 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 434

Query: 431 IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 490
           I  P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G
Sbjct: 435 IEEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMG 494

Query: 491 PRNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLV-----------------SSSDNSN 550
            R VL+++L +S       R++ WD+L  +  K  V                 ++   S 
Sbjct: 495 MRKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSK 554

Query: 551 ADDSSS----GSRNIQGNKLAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKY 582
            D +S+    G       KL  LVDT E+ +GP + PG+W+VTGAKL VE GKI ++ KY
Sbjct: 555 IDINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 603

BLAST of MC00g0469 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.2e-95
Identity = 221/633 (34.91%), Postives = 329/633 (51.97%), Query Frame = 0

Query: 3   DTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGST----IVEVDDEHTRDICLYDDV 62
           D G  + T   AV++LG+GFD+  D RL YCK   GS     +V +D    R++ +    
Sbjct: 4   DGGDVIET---AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFG 63

Query: 63  VLLNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGF-NGSKQ 122
           V  NVS DI   +       S I  F++M  YFNQ+  ++    SG+FN+ FGF +GS  
Sbjct: 64  VFQNVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWA 123

Query: 123 IDAATTKNLSMDGFYIPLAKFQF-TKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVI 182
            DAA  K+L +D   + L          L L + V+ A+P+SWDP  LA FIE +GTHVI
Sbjct: 124 TDAANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVI 183

Query: 183 TSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGG 242
           T +++GG+DV+ V+Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+  
Sbjct: 184 TGVSVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKA 243

Query: 243 LFNNQ---------GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVR 302
             ++           V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV 
Sbjct: 244 YHHSHSQPKFPEAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVP 303

Query: 303 LSPDVIEMTFFPITALLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRD 362
             PD I   F PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV +
Sbjct: 304 DKPDAINFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHN 363

Query: 363 TVP--GHQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQ 422
            +P           P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQ
Sbjct: 364 DLPFGAAPNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQ 423

Query: 423 HLASL-PKILQPYWDTHVGIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETF 482
           HL +    + +   D H+      W+G ++   + R+FEP+  K FSHV T P++    +
Sbjct: 424 HLDNTRTTVGEKITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNW 483

Query: 483 MGELSG-------VYIVTGAQLGVWDFGPRNVLYMKLLYSRLPGCTIRRTLWDH------ 542
           +   S         +IVTGAQL V   G ++VL+++L Y+++    + +  W H      
Sbjct: 484 IKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTS 543

Query: 543 ----LLNDKSKKLVSSSDNSNADDSS------------SGSRNIQGNKLAKLVDTSEMSK 583
               + +  S  L S S + N                  G      NK+ K VD S++ +
Sbjct: 544 QKSGIFSSMSMPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCR 603

BLAST of MC00g0469 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1187 bits (3072), Expect = 0.0
Identity = 582/583 (99.83%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAK 540
           RNVLYMKLLYSRLPGCTI+RTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAK
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAK 540

Query: 541 LVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           LVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of MC00g0469 vs. NCBI nr
Match: XP_038882143.1 (MACPF domain-containing protein CAD1-like [Benincasa hispida])

HSP 1 Score: 1103 bits (2853), Expect = 0.0
Identity = 537/584 (91.95%), Postives = 561/584 (96.06%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           MG++GTALHTA+NAVQALGRGFDVNFD+RLLYCKGVSGSTIVE+DDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTAMNAVQALGRGFDVNFDSRLLYCKGVSGSTIVEIDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFNSAFGF G KQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNSAFGFTGFKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           A TK+LS DGFYIPLAKFQFTKTPLVLQENVKRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 AATKSLSTDGFYIPLAKFQFTKTPLVLQENVKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIY+KQHQSSPLSTME+KRYVQDIANQRFSDTQG  GSGSIK KEKGLDGGLFNN
Sbjct: 181 IGGKDVIYIKQHQSSPLSTMELKRYVQDIANQRFSDTQGQMGSGSIKHKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGG+DLEQNHVRWAR VR SPDVIEMTF PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGNDLEQNHVRWARNVRSSPDVIEMTFCPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYL+YKPPLEELRYFLEFQIPRVWAP  DT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLDYKPPLEELRYFLEFQIPRVWAPTCDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSH+STAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHISTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSN-ADDSSSGSRNIQGNKLA 540
           RNVLYMKLLYSRLPGCTIRR LWDHL NDKSKKLV+SSDNS+  DDSSSG+++IQGNKLA
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRMLWDHLPNDKSKKLVTSSDNSSKVDDSSSGTKSIQGNKLA 540

Query: 541 KLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           K+VDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 KVVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584

BLAST of MC00g0469 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 536/585 (91.62%), Postives = 556/585 (95.04%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFN+AFGF GSKQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           A  KNLS DGFYIPLAKFQ T T L+L ENVKR++PTSWDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG  GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKILQPYWDTH+ I
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSNA--DDSSSGSRNIQGNKL 540
           RNVLYMKLLYSRLPGCTIRRTLWDHL NDKSKKLVSSSDNSNA  DDSSSGS+NIQGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           AKLVDT+EMSKGP D PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of MC00g0469 vs. NCBI nr
Match: XP_023517935.1 (MACPF domain-containing protein CAD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 535/585 (91.45%), Postives = 555/585 (94.87%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFN+AFGF GSKQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           A  KNLS DGFYIPLAKFQ T T L+L ENVKR++PTSWDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG  GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKILQPYWDTH+ I
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSN--ADDSSSGSRNIQGNKL 540
           R VLYMKLLYSRLPGCTIRRTLWDHL NDKSKKLVSSSDNSN  ADDSSSGS+NIQGNKL
Sbjct: 481 RYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNTAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           AKLVDT+EMSKGP D PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of MC00g0469 vs. NCBI nr
Match: XP_008463260.1 (PREDICTED: MACPF domain-containing protein CAD1-like [Cucumis melo] >TYK09776.1 MACPF domain-containing protein CAD1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1089 bits (2817), Expect = 0.0
Identity = 536/586 (91.47%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFNSAFGF G +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+EN+KRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLN-DKSKKLVSSSDNSN-ADDSSSGSRNIQGN-K 540
           RNVLYMKLLYSRLPGCTIRRTLWDHL   +KSKKLVSSSDN+N AD+SSSG+++ QGN K
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of MC00g0469 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1187 bits (3072), Expect = 0.0
Identity = 582/583 (99.83%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT
Sbjct: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF
Sbjct: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAK 540
           RNVLYMKLLYSRLPGCTI+RTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAK
Sbjct: 481 RNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAK 540

Query: 541 LVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           LVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583

BLAST of MC00g0469 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 536/585 (91.62%), Postives = 556/585 (95.04%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKNFQ+PIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFN+AFGF GSKQIDA
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           A  KNLS DGFYIPLAKFQ T T L+L ENVKR++PTSWDPP+LASFIENFGTHVITSIT
Sbjct: 121 AAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG  GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAI LYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHLQHLA+LPKILQPYWDTH+ I
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLQHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSNA--DDSSSGSRNIQGNKL 540
           RNVLYMKLLYSRLPGCTIRRTLWDHL NDKSKKLVSSSDNSNA  DDSSSGS+NIQGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNAAADDSSSGSKNIQGNKL 540

Query: 541 AKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           AKLVDT+EMSKGP D PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of MC00g0469 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1089 bits (2817), Expect = 0.0
Identity = 536/586 (91.47%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFNSAFGF G +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+EN+KRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLN-DKSKKLVSSSDNSN-ADDSSSGSRNIQGN-K 540
           RNVLYMKLLYSRLPGCTIRRTLWDHL   +KSKKLVSSSDN+N AD+SSSG+++ QGN K
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of MC00g0469 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1089 bits (2817), Expect = 0.0
Identity = 536/586 (91.47%), Postives = 559/586 (95.39%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           MG++GTALHTA NAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
            NVSWDIKNFQEPIGHRSSGICSFDEM +YFNQKDGLSEGY SGSFNSAFGF G +Q DA
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDEMADYFNQKDGLSEGYPSGSFNSAFGFTGLRQTDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
           + TK+LS DGFYIPLAKFQFTKTPL L+EN+KRA+PT+WDPPSLASFIENFGTHVITSIT
Sbjct: 121 SATKSLSTDGFYIPLAKFQFTKTPLELKENIKRAVPTTWDPPSLASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG TGSGSIKLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQTGSGSIKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVR SPDVIEMTF+PITALL
Sbjct: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRSSPDVIEMTFYPITALL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAP+ D +PG QRKEPVCPSLQF
Sbjct: 301 DGVVGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPIHDKLPGRQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKI+ PYWDTHV I
Sbjct: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKIMHPYWDTHVAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLN-DKSKKLVSSSDNSN-ADDSSSGSRNIQGN-K 540
           RNVLYMKLLYSRLPGCTIRRTLWDHL   +KSKKLVSSSDN+N AD+SSSG+++ QGN K
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKSKKLVSSSDNTNNADNSSSGTKSTQGNNK 540

Query: 541 LAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           LAKLVDT+EMSKGP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 541 LAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 586

BLAST of MC00g0469 vs. ExPASy TrEMBL
Match: A0A6J1HR44 (MACPF domain-containing protein CAD1-like OS=Cucurbita maxima OX=3661 GN=LOC111465969 PE=4 SV=1)

HSP 1 Score: 1088 bits (2814), Expect = 0.0
Identity = 532/585 (90.94%), Postives = 554/585 (94.70%), Query Frame = 0

Query: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDA 120
           LNVSWDIKN Q+PIGHRSSGICSFDEMV+YFNQKDGLSEGY SGSFN+AFGF GSKQIDA
Sbjct: 61  LNVSWDIKNSQQPIGHRSSGICSFDEMVDYFNQKDGLSEGYPSGSFNTAFGFTGSKQIDA 120

Query: 121 ATTKNLSMDGFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSIT 180
              KNLS DGFYIPLAKFQ T T L+L ENVKR++PTSWDPP+LASFIENFGTHVITSIT
Sbjct: 121 DAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPALASFIENFGTHVITSIT 180

Query: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNN 240
           IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG  GSGS KLKEKGLDGGLFNN
Sbjct: 181 IGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGSGSRKLKEKGLDGGLFNN 240

Query: 241 QGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALL 300
           QGVYPQPTSAPY +GKEDVTVIFRRRGGDDLEQNHV+WA+TVR SPDVIEMTF+PIT+LL
Sbjct: 241 QGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQNHVKWAKTVRSSPDVIEMTFYPITSLL 300

Query: 301 DGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQF 360
           DGV GKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDT+PGHQRKEPVCPSLQF
Sbjct: 301 DGVAGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTIPGHQRKEPVCPSLQF 360

Query: 361 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGI 420
           SIMGQKLYVSIEQI IGRKPVTGM LSLEG KQNRLSIHL+HLA+LPKILQPYWDTH+ I
Sbjct: 361 SIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQNRLSIHLKHLATLPKILQPYWDTHIAI 420

Query: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGP 480
           GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVW FG 
Sbjct: 421 GAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWGFGS 480

Query: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSN--ADDSSSGSRNIQGNKL 540
           RNVLYMKLLYSRLPGCTIRRTLWDHL NDKSKKLVSSSDNSN  ADDSSSGS+N QGNKL
Sbjct: 481 RNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKLVSSSDNSNYAADDSSSGSKNTQGNKL 540

Query: 541 AKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 583
           AKLVDT+EMSKGP D PGHWLVTGAKLGVEK +IVLRVKYSLLNY
Sbjct: 541 AKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRIVLRVKYSLLNY 585

BLAST of MC00g0469 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 691.4 bits (1783), Expect = 6.1e-199
Identity = 336/574 (58.54%), Postives = 424/574 (73.87%), Query Frame = 0

Query: 10  TAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVLLNVSWDIKN 69
           T  NA+QALGRGFDV  D RLLYCKG  GS +V +++   RD+ L    +L NV  DI  
Sbjct: 20  TLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPNVPADIDC 79

Query: 70  FQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMD 129
            +   G +   +CSF EM   FN + G+      G FN+ F + GS Q+DAA+TK+L++ 
Sbjct: 80  SRGNSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALV 139

Query: 130 GFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYV 189
           G++IPL   +  K  LVL   ++RA+P+SWDP SLASFIEN+GTH++TS+TIGG+DV+Y+
Sbjct: 140 GYFIPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYI 199

Query: 190 KQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGGLFNNQGVYPQPTS 249
           +QHQSSPL   E++ YV D+   RF + +  + +G +K K+K                  
Sbjct: 200 RQHQSSPLPVSEIENYVNDMIKHRFHEAESQSITGPLKYKDK------------------ 259

Query: 250 APYLTGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALLDGVTGKEHL 309
                   D+TVIFRRRGGDDLEQ+H RWA TV  +PD+I MTF PI +LL+GV G  HL
Sbjct: 260 --------DITVIFRRRGGDDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHL 319

Query: 310 TRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVPGHQRKEPVCPSLQFSIMGQKLYV 369
           TRAI LYLEYKPP+E+L+YFL++QI R WAP +  +   QRKEPVC SLQFS+MG KL++
Sbjct: 320 TRAIELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFI 379

Query: 370 SIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGIGAPKWQGPE 429
           S +Q+++GRKPVTG+RLSLEGSKQNRLSIHLQHL SLPKILQP+WD+HV IGAPKWQGPE
Sbjct: 380 SADQVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPE 439

Query: 430 EQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGPRNVLYMKLL 489
           EQDSRWFEP+KWKNFSHVST+PIE+ ET +G+LSGV+IVTGAQLGVWDFG +NVL++KLL
Sbjct: 440 EQDSRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLL 499

Query: 490 YSRLPGCTIRRTLWDHLLNDKSKKLVSSSDNSNADDSSSGSRNIQGNKLAKLVDTSEMSK 549
           +S++PGCTIRR++WDH     S +L     +++   SS+   + Q  KLAK+VD+SEM K
Sbjct: 500 FSKVPGCTIRRSVWDHTPVASSGRLEPGGPSTS---SSTEEVSGQSGKLAKIVDSSEMLK 559

Query: 550 GPHDPPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 584
           GP D PGHWLVTGAKLGVEKGKIVLRVKYSLLNY
Sbjct: 560 GPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLLNY 561

BLAST of MC00g0469 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 422.5 bits (1085), Expect = 5.3e-118
Identity = 251/599 (41.90%), Postives = 359/599 (59.93%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVS-GSTIVEV-DDEHTRDICLYDDVVLLNVSWDIKNFQ 73
           A+ ++G G+D+  D RL YCKG S  S ++++ + +   +I L   + + NVS  IK  +
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 74  EPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMDGF 133
                  S I  F +M   FNQ+  L+    SG FN+ F F+   Q DAA TKNL+ DG 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 134 YIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYVKQ 193
           +I L      K+ ++L+E+VK+A+P++WDP +LA FI+ +GTH+I S+ +GGKDVIY KQ
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 194 HQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGS--------IKLKEKGLDGGLFNNQGV 253
             SS L   ++++ ++++A++RF +   +  +GS        ++ KE+ L     ++ G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 254 YPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRLSPDVIEMTFFPITALLDG 313
           Y           KED   + +RRGG+D     H  W +TV++ PDVI M+F PIT+LL+G
Sbjct: 254 Y---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 313

Query: 314 VTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVP-GHQRKEPVCPSLQFS 373
           V G   L+ AI LYL YKPP+EEL  FLEFQ+PR WAPV   +P G QRK+  C SLQFS
Sbjct: 314 VPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCASLQFS 373

Query: 374 IMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQPYWDTHVGIG 433
             G KLYV+   + +G++P+TGMRL LEG + NRL+IHLQHL+SLPKI Q   D +  I 
Sbjct: 374 FFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLNRSI- 433

Query: 434 APKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFGPR 493
                  E  D R++E V WKN+SHV T P+E+ +    +LS   +VTGAQL V   G +
Sbjct: 434 -----RQESHDRRYYEKVNWKNYSHVCTEPVESDD----DLS---VVTGAQLHVESHGFK 493

Query: 494 NVLYMKLLYSRLPGCT-IRRTLWDHLLNDKSKK-----LVS-------------SSDNSN 553
           NVL+++L +SR+ G T ++ + WD  +    K      L+S             +  N N
Sbjct: 494 NVLFLRLCFSRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNIN 553

Query: 554 ADDSSSGSR-NIQGNKLAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKYSL 581
           +     G     Q  KL K VDTSEM++GP + PG+W+V+GA+L VEKGKI L+VKYSL
Sbjct: 554 SAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSL 590

BLAST of MC00g0469 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 379.0 bits (972), Expect = 6.8e-105
Identity = 237/603 (39.30%), Postives = 332/603 (55.06%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGV-SGSTIVEVDDEHTRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMD 130
            +       S I SF++M   FNQ   LS    SG FN+ F F+     DA++ K L+ D
Sbjct: 75  DKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASSVKTLAYD 134

Query: 131 GFYIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYV 190
           G++I L   +  +  L L++ VKR +P+SWD  +LA FIE +GTHV+  +T+GGKDVI+V
Sbjct: 135 GWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMGGKDVIHV 194

Query: 191 KQHQSSPLSTMEVKRYVQDIANQRFS----DTQGLTGSGSIKLKEKGLDGGLFNNQGVYP 250
           KQ + S     E+++ ++   ++RF     +++      S K KE+ L        G+ P
Sbjct: 195 KQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENL-----LQWGLQP 254

Query: 251 QPTSAPYL----TGKEDVTVIFRRRGGDDLEQNHVRWARTVRLSPDVIEMTFFPITALLD 310
             TS        T  E++  +  RRGG DL Q+H RW  TV  +P+VI M F PIT+LL 
Sbjct: 255 FGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQSHERWLSTVSQAPNVISMCFVPITSLLS 314

Query: 311 GVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRDTVP-GHQRKEPVCPSLQF 370
           G+ G   L+ A+ LYL YKPP+EEL  FLEFQ+PR WAPV   +P G +R +   PSLQF
Sbjct: 315 GLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPSLQF 374

Query: 371 SIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQHLASLPKILQ-PYWDTHVG 430
           S+MG KLYV+  ++  G +PVTG+R  LEG K N L+IHLQHL++ P  L   + DT+  
Sbjct: 375 SLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDTYEP 434

Query: 431 IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIENPETFMGELSGVYIVTGAQLGVWDFG 490
           I  P  +G       ++ PVKW  FSHV T P++       + +   IVT A L V   G
Sbjct: 435 IEEPVEKG-------YYVPVKWGIFSHVCTYPVQYNGARSDDTAS--IVTKAWLEVKGMG 494

Query: 491 PRNVLYMKLLYSRLPGCTIRRTLWDHLLNDKSKKLV-----------------SSSDNSN 550
            R VL+++L +S       R++ WD+L  +  K  V                 ++   S 
Sbjct: 495 MRKVLFLRLGFSLDASAVTRKSCWDNLSTNSRKSGVFSMISTRLSTGLSPNPATTKPQSK 554

Query: 551 ADDSSS----GSRNIQGNKLAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKGKIVLRVKY 582
            D +S+    G       KL  LVDT E+ +GP + PG+W+VTGAKL VE GKI ++ KY
Sbjct: 555 IDINSAVYPRGPSPPVKPKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKY 603

BLAST of MC00g0469 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 352.1 bits (902), Expect = 8.8e-97
Identity = 221/633 (34.91%), Postives = 329/633 (51.97%), Query Frame = 0

Query: 3   DTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGST----IVEVDDEHTRDICLYDDV 62
           D G  + T   AV++LG+GFD+  D RL YCK   GS     +V +D    R++ +    
Sbjct: 4   DGGDVIET---AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFG 63

Query: 63  VLLNVSWDIKNFQEPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGF-NGSKQ 122
           V  NVS DI   +       S I  F++M  YFNQ+  ++    SG+FN+ FGF +GS  
Sbjct: 64  VFQNVSADINCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWA 123

Query: 123 IDAATTKNLSMDGFYIPLAKFQF-TKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVI 182
            DAA  K+L +D   + L          L L + V+ A+P+SWDP  LA FIE +GTHVI
Sbjct: 124 TDAANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVI 183

Query: 183 TSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGSIKLKEKGLDGG 242
           T +++GG+DV+ V+Q +SS L    ++ ++ D+ +Q F        +GS  L  + L+  
Sbjct: 184 TGVSVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLF--------TGSCLLSTRRLNKA 243

Query: 243 LFNNQ---------GVYPQPTSAPY----LTGKEDVTVIFRRRGGDDLEQNHVRWARTVR 302
             ++           V+    +  +    +  +  +TVI  +RGGD   ++H  W  TV 
Sbjct: 244 YHHSHSQPKFPEAFNVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVP 303

Query: 303 LSPDVIEMTFFPITALLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEFQIPRVWAPVRD 362
             PD I   F PIT+LL  V G   L+ A+ LYL YKPPL +L+YFL+F  PR WAPV +
Sbjct: 304 DKPDAINFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHN 363

Query: 363 TVP--GHQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSKQNRLSIHLQ 422
            +P           P+L  + MG KLYV+   ++  + PVTGMR  LEG K NRL+IHLQ
Sbjct: 364 DLPFGAAPNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQ 423

Query: 423 HLASL-PKILQPYWDTHVGIGAPKWQGPEE--QDSRWFEPVKWKNFSHVSTAPIENPETF 482
           HL +    + +   D H+      W+G ++   + R+FEP+  K FSHV T P++    +
Sbjct: 424 HLDNTRTTVGEKITDEHI------WRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNW 483

Query: 483 MGELSG-------VYIVTGAQLGVWDFGPRNVLYMKLLYSRLPGCTIRRTLWDH------ 542
           +   S         +IVTGAQL V   G ++VL+++L Y+++    + +  W H      
Sbjct: 484 IKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTS 543

Query: 543 ----LLNDKSKKLVSSSDNSNADDSS------------SGSRNIQGNKLAKLVDTSEMSK 583
               + +  S  L S S + N                  G      NK+ K VD S++ +
Sbjct: 544 QKSGIFSSMSMPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPVPANNKIVKFVDLSQLCR 603

BLAST of MC00g0469 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 207.2 bits (526), Expect = 3.5e-53
Identity = 125/331 (37.76%), Postives = 192/331 (58.01%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVS-GSTIVEV-DDEHTRDICLYDDVVLLNVSWDIKNFQ 73
           A+ ++G G+D+  D RL YCKG S  S ++++ + +   +I L   + + NVS  IK  +
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIKCDK 73

Query: 74  EPIGHRSSGICSFDEMVNYFNQKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMDGF 133
                  S I  F +M   FNQ+  L+    SG FN+ F F+   Q DAA TKNL+ DG 
Sbjct: 74  GERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQKDAAYTKNLAFDGV 133

Query: 134 YIPLAKFQFTKTPLVLQENVKRAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYVKQ 193
           +I L      K+ ++L+E+VK+A+P++WDP +LA FI+ +GTH+I S+ +GGKDVIY KQ
Sbjct: 134 FISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVSVKMGGKDVIYAKQ 193

Query: 194 HQSSPLSTMEVKRYVQDIANQRFSDTQGLTGSGS--------IKLKEKGLDGGLFNNQGV 253
             SS L   ++++ ++++A++RF +   +  +GS        ++ KE+ L     ++ G 
Sbjct: 194 QHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKEQRLRFADTSSLGS 253

Query: 254 YPQPTSAPYLTGKEDVTVIFRRRGGDDLEQ-NHVRWARTVRLSPDVIEMTFFPITALLDG 313
           Y           KED   + +RRGG+D     H  W +TV++ PDVI M+F PIT+LL+G
Sbjct: 254 Y---------ANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITSLLNG 313

Query: 314 VTGKEHLTRAILLYLEYKP--PLEELRYFLE 332
           V G   L+ AI LYL  KP   L  + +FLE
Sbjct: 314 VPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335