Homology
BLAST of MC00g0428 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 890.6 bits (2300), Expect = 2.5e-257
Identity = 439/743 (59.08%), Postives = 545/743 (73.35%), Query Frame = 0
Query: 2 SYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMI 61
SY+VY G+HSH + ++ E+HY+ LGS GS E A +AIFYSY +HI+GFAA +
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 62 EEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIG 121
+ +A +++K+P V SV NKA KLHTT SW+FLGLEH+ +PS+SIW A FGE TII
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 122 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEG--GSKFHCNRKLIGARYFNKGYAAYAGPL 181
NLDTGVWPESKSF D+G GPIPSRW+G C+ + FHCNRKLIGARYFNKGYAA G L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 182 NASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFS 241
N+S+++ RD DGHG+HTLSTA G+FV G S+FG GNGTAKGGSPRARV AYKVCWP V
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPV-K 270
Query: 242 GGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGN 301
G EC+DAD+LAA +AAI DG DV+SVSLGG+ F +D AIG+FHA + ++VVCSAGN
Sbjct: 271 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 330
Query: 302 SGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISA 361
SGPA TV N+APW +TVGAST++R F S + LGN KH KG SLS LP KFYP++++
Sbjct: 331 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 390
Query: 362 VDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANA 421
V+AKAKN SA +AQ+C GSLDP K KGKI+VCLRG+NGRV+KG A G +GM+L N
Sbjct: 391 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 450
Query: 422 QENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFS 481
G++LLAD H+LPA+ ++ D V +YI TK P+A++T RT+LG+KPAP+MASFS
Sbjct: 451 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 510
Query: 482 SRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISG 541
S+GP+ + ILKPDITAPGV++IAA++ P+ FD RR+ +N +SGTSMSCPHISG
Sbjct: 511 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 570
Query: 542 IVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVN 601
I GLLKT YP WSPAAI+SAIMTTA + PI N KA P ++GAGHV+P+ AVN
Sbjct: 571 IAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVN 630
Query: 602 PGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQ-SFKLTDFNYPSISIPNLKSGP 661
PGLVYDL DYLNFLC+ GYN +QI FS +F CS L + NYPSI++PNL S
Sbjct: 631 PGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSK 690
Query: 662 VTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRG 721
VT+ R VKNVG Y +V P GV V+V+P L FT++ E+K FKV++ + +G
Sbjct: 691 VTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG 750
Query: 722 YVFGSLAWSDGKRHVRSPIAVNL 742
YVFG L WSD K VRSPI V L
Sbjct: 751 YVFGELVWSDKKHRVRSPIVVKL 772
BLAST of MC00g0428 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 884.4 bits (2284), Expect = 1.8e-255
Identity = 451/745 (60.54%), Postives = 548/745 (73.56%), Query Frame = 0
Query: 738 AVNLSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGF 797
A+ SYI YLGSH+H S+ L SH L S +GS+ AK+AIFYSY ++INGF
Sbjct: 37 ALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGF 96
Query: 798 AAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDGEIPSNSIWNLASFGES 857
AAILDE A E+AKHP VVSV NK RKLHTT SW+F+ L +G + +S+WN A +GE
Sbjct: 97 AAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGED 156
Query: 858 TIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCNRKLIGARYFNKGYVASIG 917
TII NLDTGVWPESKSFSD+GYG +P+RW+G C CNRKLIGARYFNKGY+A G
Sbjct: 157 TIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTG 216
Query: 918 -PLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPA 977
P NASYET RD +GHG+HTLSTA GNFV GA+VFG GNGTA GGSP+ARVAAY+VCWP
Sbjct: 217 LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPP 276
Query: 978 VLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSA 1037
V CF ADILA EAAI DGVDVLS S+GG ++ DG+AIG+FHAV++G+TVVCSA
Sbjct: 277 VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSA 336
Query: 1038 GNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLI 1097
GNSGP GTVSNVAPW+ITVGAS+ DR F ++V L N + KG SLS K LP +K Y LI
Sbjct: 337 GNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLI 396
Query: 1098 STADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILA 1157
S ADA +N ++ A LC++GSLDPKKV+GKI+VCLRGDNARVDKG AA AGA GM+L
Sbjct: 397 SAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLC 456
Query: 1158 NAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMAS 1217
N + +G+E+++DAH+LPAS + Y DGE +F Y+ STK P Y+ L KPAPFMAS
Sbjct: 457 NDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMAS 516
Query: 1218 FSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHV 1277
FSSRGPNTI ILKPDI APGV+IIAA++E P+ D RR+PFN ESGTSMSCPH+
Sbjct: 517 FSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHI 576
Query: 1278 SGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKA 1337
SG+VGLLKT +P WSPAAI+SA+MTT+ T+ N P+++ + KANP SYG+GHV+PNKA
Sbjct: 577 SGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKA 636
Query: 1338 LNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAP-FACSHSFKLTDFNYPSISIPNLKF 1397
+PGLVYDLT DYL+FLCA GYN T ++ F+ P + C L DFNYPSI++PNL
Sbjct: 637 AHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLT- 696
Query: 1398 GPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGEEQSFRVVVRRVENEER 1457
G + R++KNVG P TY A+ + P GV VSVEP L F GE + F++ +R +
Sbjct: 697 GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLP-VTP 756
Query: 1458 RGYVFGWLAWSDGNHRVRSPIAVNL 1481
GYVFG L W+D +H VRSPI V L
Sbjct: 757 SGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of MC00g0428 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 762.3 bits (1967), Expect = 1.0e-218
Identity = 419/761 (55.06%), Postives = 512/761 (67.28%), Query Frame = 0
Query: 3 YIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMIE 62
YIVY+G+HSHG SP++ DLELAT+SHY+LLGS+ GS E AKEAI YSYNRHI+GFAA++E
Sbjct: 32 YIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLE 91
Query: 63 EKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIGN 122
E+ A D+AKNP V SV +K KLHTT SW FLGL G NS W FGE+TIIGN
Sbjct: 92 EEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENTIIGN 151
Query: 123 LDTGVWPESKSFSDKGYGPIPSRWRGS-CE-----GGSKFHCNRKLIGARYFNKGYAAYA 182
+DTGVWPES+SFSDKGYG +PS+WRG C+ G K CNRKLIGARY+NK + A+
Sbjct: 152 IDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHN 211
Query: 183 GPLNASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQ 242
G L+ TARD GHGTHTLSTAGGNFV GA VF GNGTAKGGSPRARV AYKVCW
Sbjct: 212 GQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCW-S 271
Query: 243 VFSGGECFDADILAAIEAAISDGVDVLSVSLGGD----SKDFSDDVTAIGAFHAVQNGVI 302
+ C+ AD+LAAI+ AI DGVDV++VS G ++ D +IGAFHA+ ++
Sbjct: 272 LTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNIL 331
Query: 303 VVCSAGNSGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQK 362
+V SAGN GP PGTV N+APW+ T+ AST++R F+S + + N+ I+G SL + P Q
Sbjct: 332 LVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQL-IEGASLFVNLPPNQA 391
Query: 363 FYPLISAVDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLR-GENGRVDKGHVAAQAGA 422
F LI + DAK N + +AQ+C G+LD KV GKI++C R G+ V +G A AGA
Sbjct: 392 F-SLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGA 451
Query: 423 VGMILANAQENGDELLADAHLLPASHVSYADGELVFQYIKST-----KNPM-----AYMT 482
GMIL N +NG L A+ H+ + + +K+T +P+ M+
Sbjct: 452 RGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMS 511
Query: 483 HVRTELGIKPAPLMASFSSRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRR 542
RT G KPAP+MASFSSRGPN I+ SILKPD+TAPGVNI+AA+SE A S DNRR
Sbjct: 512 RARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRR 571
Query: 543 -IPYNVVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTI-K 602
+NV+ GTSMSCPH SGI GLLKT +P WSPAAIKSAIMTTA T N PI + K
Sbjct: 572 GFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDK 631
Query: 603 HKANPLAYGAGHVRPSRAVNPGLVYDLSTNDYLNFLCARGYNQTQIRKFS-NTSFVCSQS 662
A+ AYG+GHVRP A+ PGLVYDLS DYLNFLCA GY+Q I + N +F+CS S
Sbjct: 632 TLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGS 691
Query: 663 FKLTDFNYPSISIPNLKSGPVTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRI 722
+ D NYPSI++PNL+ PVTI R V NVG TY + P G S++V P L FT+I
Sbjct: 692 HSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKI 751
Query: 723 DEEKAFKVVVRRVGKGKRRGYVFGSLAWSDGKRHVRSPIAV 740
E K FKV+V+ RR Y FG L W+DGK VRSPI V
Sbjct: 752 GERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786
BLAST of MC00g0428 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 627.5 bits (1617), Expect = 3.9e-178
Identity = 346/748 (46.26%), Postives = 475/748 (63.50%), Query Frame = 0
Query: 2 SYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMI 61
+YIV++ + PS+ DL H N S L S + E + Y+Y I GF+ +
Sbjct: 31 TYIVHM---AKSQMPSSFDL------HSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRL 90
Query: 62 EEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIG 121
++ A+ L P V SV +LHTT + FLGL+ + ++ A ++G
Sbjct: 91 TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----TADLFPEAGSYSDVVVG 150
Query: 122 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFH---CNRKLIGARYFNKGYAAYAGP 181
LDTGVWPESKS+SD+G+GPIPS W+G CE G+ F CNRKLIGAR+F +GY + GP
Sbjct: 151 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 210
Query: 182 LNASYE--TARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQ 241
++ S E + RD DGHGTHT STA G+ V GAS+ G +GTA+G +PRARV YKVCW
Sbjct: 211 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL- 270
Query: 242 VFSGGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCS 301
G CF +DILAAI+ AI+D V+VLS+SLGG D+ D AIGAF A++ G++V CS
Sbjct: 271 ----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 330
Query: 302 AGNSGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPL 361
AGN+GP+ ++ N+APW+ TVGA T++R F + LGN K+ G SL K P
Sbjct: 331 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 390
Query: 362 ISAVDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMIL 421
I A +A +A +C G+L P+KVKGKI++C RG N RV KG V AG VGMIL
Sbjct: 391 IYAGNAS----NATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMIL 450
Query: 422 ANAQENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMA 481
AN NG+EL+ADAHLLPA+ V G+++ Y+ + NP A ++ + T +G+KP+P++A
Sbjct: 451 ANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVA 510
Query: 482 SFSSRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPH 541
+FSSRGPN+I +ILKPD+ APGVNI+AA++ A P+G + D+RR+ +N++SGTSMSCPH
Sbjct: 511 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 570
Query: 542 ISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHK-ANPLAYGAGHVRPS 601
+SG+ LLK+++P+WSPAAI+SA+MTTA + P+++ K + P +GAGHV P+
Sbjct: 571 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPT 630
Query: 602 RAVNPGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVC--SQSFKLTDFNYPSISIPN 661
A NPGL+YDL+T DYL FLCA Y QIR S ++ C S+S+ + D NYPS ++
Sbjct: 631 TATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNV 690
Query: 662 LKSGPVTIRRRVKNVGSAGTYVARV-KVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVG 721
G R V +VG AGTY +V GV +SVEP L F +E+K++ V
Sbjct: 691 DGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDS 750
Query: 722 KGKRRGYVFGSLAWSDGKRHVRSPIAVN 741
FGS+ WSDGK V SP+A++
Sbjct: 751 SKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of MC00g0428 vs. ExPASy Swiss-Prot
Match:
Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)
HSP 1 Score: 583.9 bits (1504), Expect = 4.9e-165
Identity = 345/777 (44.40%), Postives = 460/777 (59.20%), Query Frame = 0
Query: 743 YIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAILD 802
YI Y G H I+ E H++ L S+ S A+ ++ YSY INGFAA L
Sbjct: 27 YIVYFGEHKGDKAFHEIE-----EHHHSYLQSVKESEEDARASLLYSYKHSINGFAAELT 86
Query: 803 EKVAQELAKHPSVVSVHENKARK--LHTTGSWSFLGL---ENDGEIP------------S 862
A +L K VVSV ++ RK HTT SW F+GL E D ++P
Sbjct: 87 PDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVG 146
Query: 863 NSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKF---RCNRKL 922
+ A G+ I+G LD+GVWPESKSF+DKG GP+P W+G C+ G F CNRK+
Sbjct: 147 RNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNRKI 206
Query: 923 IGARYFNKGYVASIGPLNAS----YETARDDEGHGTHTLSTAGGNFVSGASVFGN-GNGT 982
IGARY+ KGY G NA+ + + RD +GHG+HT STA G V GAS G G+
Sbjct: 207 IGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAKGS 266
Query: 983 AKGGSPRARVAAYRVCW-----PAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGS-PE 1042
A GG+P AR+A Y+ CW V C D+LA + AI DGV V+S+S+G + P
Sbjct: 267 ASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTEPF 326
Query: 1043 EFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGL 1102
F+ DG+A+GA HAV+ I V SAGNSGP GT+SN+APW+ITVGAST DR F + L
Sbjct: 327 PFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGLVL 386
Query: 1103 GNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVC 1162
GN IK S++ KF PL+ ++ ++L C SL P+ V GK+V+C
Sbjct: 387 GNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVVLC 446
Query: 1163 LRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKS 1222
LRG +R+ KG +AG GMIL N NG+E+ +D+H +P + V+ + I +YIK+
Sbjct: 447 LRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYIKT 506
Query: 1223 TKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASP 1282
K P A++ KT + AP M FSSRGPN ++ +ILKPDI APG+ I+AA+S SP
Sbjct: 507 DKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGADSP 566
Query: 1283 SGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLH 1342
S S D+R + +N SGTSMSCPHV+G + LLK +PKWS AAI+SALMTTA +
Sbjct: 567 SKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDKKK 626
Query: 1343 PILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAP 1402
PI +TT L ANP + G+GH RP KA +PGLVYD + + YL + C+ N T I P
Sbjct: 627 PIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCS--VNITNIDPTFKCP 686
Query: 1403 FACSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVG---SPGTYVAQVKAPPGVAVSVE 1462
+ + NYPSI++PNLK V KR V NVG S TY+ VK P G++V
Sbjct: 687 SKIPPGY---NHNYPSIAVPNLK-KTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAI 746
Query: 1463 PNMLKFTGIGEEQSFRVVVRRVENE-----ERRGYVFGWLAWSDGNHRVRSPIAVNL 1481
PN+L F IG++Q F++V++ ++N+ E+ Y FGW +W+D H VRSPIAV+L
Sbjct: 747 PNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSL 790
BLAST of MC00g0428 vs. NCBI nr
Match:
XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])
HSP 1 Score: 2382 bits (6173), Expect = 0.0
Identity = 1180/1499 (78.72%), Postives = 1322/1499 (88.19%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAM 60
QSYIVYLGSHSHGS+PSA DLE+ATESHY+LLGSLLGSNEAAKEAIFYSYNRHI+GFAA+
Sbjct: 6 QSYIVYLGSHSHGSNPSAADLEIATESHYSLLGSLLGSNEAAKEAIFYSYNRHINGFAAI 65
Query: 61 IEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTII 120
+++KV EDLA+NPAVAS+HENK RKLHTTSSW FLG+EHD GIP NSIWN ASFGESTII
Sbjct: 66 LDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFGESTII 125
Query: 121 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLN 180
GNLDTGVWPESKSF+D+GYGP+P+RW+GSCEGGSKFHCNRKLIGARYFNKGYAAYAG LN
Sbjct: 126 GNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAYAGSLN 185
Query: 181 ASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFSG 240
ASYETARD++GHGTHTLSTAGGNF+SGA+VFGNGNGTAKGGSP+A V AYKVCWPQV SG
Sbjct: 186 ASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVDSG 245
Query: 241 GECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGNS 300
G CFDADILAAIEAAISDGVD+LS+SLGG +KDFS+DVTAIGAFHAVQ G+IVVCSAGNS
Sbjct: 246 GGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVCSAGNS 305
Query: 301 GPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISAV 360
GPAPGT++N APW++TVGASTINR FTSYVALGN+KHIKG SLSDKILP QKFYPLI+A
Sbjct: 306 GPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHIKGASLSDKILPEQKFYPLINAA 365
Query: 361 DAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANAQ 420
DAKA NVS+ AQ+C GSLDPKKVKGKII+CLRGEN RVDKG+ AAQAGAVGMILANA+
Sbjct: 366 DAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMILANAE 425
Query: 421 ENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFSS 480
+NGDEL+ADAHLLP SHVSY DG+ ++QYI TK PMAYMTHVRTELGIKPAP+MASFSS
Sbjct: 426 QNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVMASFSS 485
Query: 481 RGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISGI 540
RGPNTIEESILKPDITAPGVNI+AA+SEDA PSGS FDNRRIP+N+VSGTSMSCPHISGI
Sbjct: 486 RGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCPHISGI 545
Query: 541 VGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVNP 600
VGLLKTLYP WSPAAIKSAIMTTAETRAN+ HPI+NT+ KANP AYGAGHV+P+RA+NP
Sbjct: 546 VGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPNRAMNP 605
Query: 601 GLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSGPVT 660
GLVYDL+TNDY+NFLCA+GYN++QI KFS TSFVCS+SFKLTDFNYPSISIP++KSG VT
Sbjct: 606 GLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMKSGVVT 665
Query: 661 IRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRGYV 720
I RRVKNVG TYVARVKVP GVSVSVEP LKFT IDEEK+FKVV+ V K RGYV
Sbjct: 666 INRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNKHRGYV 725
Query: 721 FGSLAWSDGKRHVRSPIAVNL-SYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLG-- 780
FGSL W DGK HVRSPI VNL SYI YLGSHSHGLNPS+ID Q+ATESHYNLLGSLLG
Sbjct: 726 FGSLIWEDGKHHVRSPIVVNLGSYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGRY 785
Query: 781 ----------SNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLH 840
SN AK+AIFYSYN++INGFAA++D+KVA++LAKHP VVSV ENK RKLH
Sbjct: 786 LHSVCCVSKYSNEEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLH 845
Query: 841 TTGSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWR 900
TT SWSFLG+E++G IPSNSIWNLASFGESTIIGNLDTGVWPE+KSF DK YGPIPSRW+
Sbjct: 846 TTNSWSFLGVESNGVIPSNSIWNLASFGESTIIGNLDTGVWPEAKSFDDKEYGPIPSRWK 905
Query: 901 GSCEGGSKFRCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSG 960
GSCEGGS F+CNRKLIGARY+NKGY GPLN+SYE+ARD EGHGTHTLSTAGGNFV
Sbjct: 906 GSCEGGSNFQCNRKLIGARYYNKGYAEIAGPLNSSYESARDHEGHGTHTLSTAGGNFVPN 965
Query: 961 ASVFGNGNGTAKGGSPRARVAAYRVCWPAV-LTGGCFMADILAGFEAAIHDGVDVLSVSL 1020
A++FG GNGTAKGGSP+A VAAY+VCWP + L G CF ADILAGFEAAI DGVDVLSVSL
Sbjct: 966 ANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVDVLSVSL 1025
Query: 1021 GGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFA 1080
GG P +F D +AIG+FHAVQ+GITVVCSAGNSGP GTVSNVAPW+ITVGASTADRL+
Sbjct: 1026 GGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGASTADRLYT 1085
Query: 1081 SYVGLGNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEG 1140
+YV +G+++H KGASLS+K LP QKFYPLI++ DAK N S +YAQLCE SLDP+KV+G
Sbjct: 1086 NYVAIGDKRHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKG 1145
Query: 1141 KIVVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIF 1200
KIVVCLRGDNAR +KGYV A+AG VGMILANA++NGD++ ADAHLLPASH++Y+DG+L++
Sbjct: 1146 KIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVY 1205
Query: 1201 QYIKSTKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYS 1260
QYI STKIPMAY+TH +TE GVKPAP MASFSSRGPN+I+ SILKPDI APG +I+AAYS
Sbjct: 1206 QYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYS 1265
Query: 1261 EEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATK 1320
E+ SPSG FDKRR PFN ESGTSMSCPHVSGIVGLLKT YPKWSPAAI+SA+MTTA TK
Sbjct: 1266 EDGSPSGLYFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTK 1325
Query: 1321 ANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKK 1380
ANDL+PIL+T Q KAN +YGAGHVRPNKA +PGLVYDL+ +DYLN+LCA GY QIK+
Sbjct: 1326 ANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQ 1385
Query: 1381 FSS-APFACSHSFKLTDFNYPSISIPNLKFG-PVKAKRRVKNVGSPGTYVAQVKAPPGVA 1440
FS+ F CS SFK+TD NYPSISIPNL+ +K KRR+KNVGSPGTYV QV AP GV+
Sbjct: 1386 FSNDTSFVCSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVS 1445
Query: 1441 VSVEPNMLKFTGIGEEQSFRVVVR-RVENEERRGYVFGWLAWSDGN-HRVRSPIAVNLG 1481
VSVEP LKFTGI EE+SFRVV++ V N YVFG L WSDGN HRVRSPI V LG
Sbjct: 1446 VSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 1504
BLAST of MC00g0428 vs. NCBI nr
Match:
KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])
HSP 1 Score: 2306 bits (5975), Expect = 0.0
Identity = 1140/1484 (76.82%), Postives = 1293/1484 (87.13%), Query Frame = 0
Query: 2 SYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMI 61
SYIVYLGSH+HGS+PSA DLE+ATESHY+LLGSLLGS+EAAKEAIFYSYNRHI+GFAA++
Sbjct: 743 SYIVYLGSHTHGSNPSAFDLEIATESHYSLLGSLLGSHEAAKEAIFYSYNRHINGFAAIL 802
Query: 62 EEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIG 121
++KV +DLA+NPAV S+HENK RKLHTTSSW FLG+EHD GIP NSIWN ASFGESTIIG
Sbjct: 803 DQKVVQDLARNPAVVSIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFGESTIIG 862
Query: 122 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLNA 181
NLDTGVWPES+SF+D+GYGP+P+R KLIGA+YFNKGYAA AG LNA
Sbjct: 863 NLDTGVWPESRSFNDEGYGPVPTR---------------KLIGAKYFNKGYAANAGSLNA 922
Query: 182 SYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFSGG 241
SYETARD++GHGTHTLSTAGGNF+SGA+VFGNGNGTAKGGSP+A V AYKVCWPQV SGG
Sbjct: 923 SYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVDSGG 982
Query: 242 ECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGNSG 301
C+DADILAA+EAAISDGVDVLS+SLGG SKDFSDDVTAIGAFHAVQ G++VVCSAGNSG
Sbjct: 983 GCYDADILAAMEAAISDGVDVLSLSLGGGSKDFSDDVTAIGAFHAVQQGIVVVCSAGNSG 1042
Query: 302 PAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISAVD 361
PAPGTV+N+APW++TVGASTINR FTS+V+LGN+KHIKG SLSDKILP QKFYPLI AVD
Sbjct: 1043 PAPGTVENVAPWIITVGASTINRDFTSFVSLGNKKHIKGASLSDKILPEQKFYPLIDAVD 1102
Query: 362 AKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANAQE 421
AKA VS+ AQ+C GSLDP+KVKGKII+CLRGEN R DKG+ A +AGAVGMILANA+E
Sbjct: 1103 AKANKVSSDIAQLCLVGSLDPEKVKGKIIICLRGENARADKGYAAVKAGAVGMILANAEE 1162
Query: 422 NGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFSSR 481
NGDE++ADAHLLP SHVSY DG+ ++QYI STK PMAYMTHVRTELGIKPAP+MASFSSR
Sbjct: 1163 NGDEIIADAHLLPVSHVSYTDGQSIYQYINSTKTPMAYMTHVRTELGIKPAPVMASFSSR 1222
Query: 482 GPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISGIV 541
GPNT+EE+ILKPDITAPGVNI+AA+SEDA PSGS FD RRIP+N+VSGTSMSCPHISGIV
Sbjct: 1223 GPNTVEETILKPDITAPGVNILAAYSEDASPSGSFFDKRRIPFNIVSGTSMSCPHISGIV 1282
Query: 542 GLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVNPG 601
GLLKTLYP WSPAAIKSAIMTTAE+RAN+ HPI N KAN AYGAGHV+P+RA+NPG
Sbjct: 1283 GLLKTLYPNWSPAAIKSAIMTTAESRANDMHPIQNGGNLKANSFAYGAGHVQPNRAMNPG 1342
Query: 602 LVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSGPVTI 661
LVYDL+T DY+NFLCA+GYN++QI KFS SFVCS+SFKLTDFNYPSISIP++KSG VTI
Sbjct: 1343 LVYDLTTKDYMNFLCAQGYNKSQISKFSAMSFVCSKSFKLTDFNYPSISIPDMKSGVVTI 1402
Query: 662 RRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRGYVF 721
+RRVKNVG TYVARVKVP GVSVSVEP LKFTRIDEEK+FKVV+ V K +GYVF
Sbjct: 1403 KRRVKNVGKPSTYVARVKVPHGVSVSVEPRTLKFTRIDEEKSFKVVIGSVANNKHKGYVF 1462
Query: 722 GSLAWSDGKRHVRSPIAVNLSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVA 781
GSL W DGK H SYI YLGSHSHG NPS+ D Q+ATESH+NLLGS LGSN
Sbjct: 1463 GSLIWEDGKHH---------SYIVYLGSHSHGFNPSSTDAQIATESHFNLLGSFLGSNEE 1522
Query: 782 AKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDG 841
AK+AIFYSYN++INGFAA++D+KVA++LAKHP VVSV ENK RKLHTT SW FLGLEN+G
Sbjct: 1523 AKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGLENNG 1582
Query: 842 EIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCNRK 901
IPSNS+WNLASFGESTIIGNLDTGVWPESKSFSDK YGPIPSRW+GSCEGGSKF CNRK
Sbjct: 1583 AIPSNSLWNLASFGESTIIGNLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFYCNRK 1642
Query: 902 LIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKGG 961
LIGARY+NKGY A +GPLN+SYE+ARD EGHGTHTLSTAGG+FV A++FG GNGTAKGG
Sbjct: 1643 LIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTAKGG 1702
Query: 962 SPRARVAAYRVCWPAVLT-GGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLAI 1021
SP+A VAAY+VCWP VL G CF ADILAGFEAAI DGVDVLSVSLGGSP +F+ D ++I
Sbjct: 1703 SPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDSISI 1762
Query: 1022 GAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKGA 1081
G+FHAVQ+GI VVCSAGNSGP G+VSNVAPW+ITVGAST DRL+ SYV +G+++H KGA
Sbjct: 1763 GSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHFKGA 1822
Query: 1082 SLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARVD 1141
S+SDK LP QKFYPLIS+ DAKA NV+ A LCEEGSLDPKKV GKI++CLRGDNARV
Sbjct: 1823 SVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNARVA 1882
Query: 1142 KGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYMT 1201
KGYVAA+AGAVGMILANA++NGDE+LADAHLLPASH++Y+DG+L++QYI STKIPMAYMT
Sbjct: 1883 KGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMAYMT 1942
Query: 1202 HVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKRR 1261
HV+TE G+KPAP MASFSSRGPNT++ SILKPDI APG +I+AAYS +ASP+G+ FDKRR
Sbjct: 1943 HVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFDKRR 2002
Query: 1262 SPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQLK 1321
PFN ESGTSMSCPHVSGIVGLLKT YPKWSPAAI+SA+MTTA TKANDL PIL+T Q K
Sbjct: 2003 VPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTNQEK 2062
Query: 1322 ANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSS-APFACSHSFK 1381
AN +YGAGHV PN+A +PGLVYDL+ KDYLN+LCA+GYN QIK+FS+ F CS SFK
Sbjct: 2063 ANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSKSFK 2122
Query: 1382 LTDFNYPSISIPNLKFG-PVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIG 1441
LTD NYPSISIP L++ VK KR++KNVGSPGTYV QVK P GV+VSVEP LKFTGI
Sbjct: 2123 LTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFTGID 2182
Query: 1442 EEQSFRVVVRRVE-NEERRGYVFGWLAWSDGNHRVRSPIAVNLG 1481
EE+SFRVV++ E N Y+FG L WSDG HRVRSPI V LG
Sbjct: 2183 EEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIVVRLG 2202
BLAST of MC00g0428 vs. NCBI nr
Match:
KAG6577540.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 2275 bits (5895), Expect = 0.0
Identity = 1125/1482 (75.91%), Postives = 1274/1482 (85.96%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAM 60
QSYIVYLGSHSHGS+PS+VDL +ATESHY+LLGSLLGSNE AKEAIFYSY+RHI+GFAA+
Sbjct: 22 QSYIVYLGSHSHGSNPSSVDLRIATESHYSLLGSLLGSNEIAKEAIFYSYHRHINGFAAV 81
Query: 61 IEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTII 120
++ KVA+DL ++PAV SVHENK RKLHTTSSW FL LE+ G P NSIWN A+FGESTII
Sbjct: 82 LDHKVAQDLTRHPAVVSVHENKMRKLHTTSSWEFLELENGEGTPPNSIWNGANFGESTII 141
Query: 121 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLN 180
NLDTGVWPESKSF+D+GYG IPS+WRGSCEGGS FHCNRKLIGARYFNKGY A AG L+
Sbjct: 142 ANLDTGVWPESKSFNDEGYGAIPSKWRGSCEGGSNFHCNRKLIGARYFNKGYTALAGSLD 201
Query: 181 ASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFSG 240
S++T RDHDGHGTHTLSTAGGNFVSGA+VFGNGNGTAKGGSP+A V AYKVCWP F G
Sbjct: 202 DSFDTTRDHDGHGTHTLSTAGGNFVSGANVFGNGNGTAKGGSPKAFVAAYKVCWPT-FHG 261
Query: 241 GECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGNS 300
G+C DADILAAIEAAI+DGVDVLS+SLG S +F DDVTAIG+FHAVQ G++VVCS GNS
Sbjct: 262 GQCSDADILAAIEAAITDGVDVLSLSLGRGSTEFFDDVTAIGSFHAVQQGIVVVCSGGNS 321
Query: 301 GPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISAV 360
GP P +V+N+APWL TV ASTI R TSYVALGN+KHI G S+SDKILPAQ+FYPLI++V
Sbjct: 322 GPDPQSVENVAPWLFTVAASTITRQLTSYVALGNKKHITGASISDKILPAQQFYPLITSV 381
Query: 361 DAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRG-ENGRVDKGHVAAQAGAVGMILANA 420
DAKA N+S A++C EGSLDP+KVKGKIIVC+RG ++ RVDKG+VAAQAGAVGMILAN+
Sbjct: 382 DAKAINISVETAKLCVEGSLDPRKVKGKIIVCVRGGDSARVDKGYVAAQAGAVGMILANS 441
Query: 421 QENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFS 480
+E+G+EL+ADAHLLP SH+SY DGE V++YI STK P+AYMTHVRTE GIKPAP+MASFS
Sbjct: 442 EEDGNELIADAHLLPVSHISYIDGETVYEYINSTKTPVAYMTHVRTETGIKPAPVMASFS 501
Query: 481 SRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISG 540
SRGPN+IEESILKPDITAPGVNIIAA+SEDA PSGS FDNRRIP+NVVSGTSMSCPHISG
Sbjct: 502 SRGPNSIEESILKPDITAPGVNIIAAYSEDASPSGSPFDNRRIPFNVVSGTSMSCPHISG 561
Query: 541 IVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVN 600
IV LLKT+Y KWSPAAIKSAIMTTAETRAN+ HPI+++ K ANPLAYGAGHV P+RA N
Sbjct: 562 IVALLKTIYSKWSPAAIKSAIMTTAETRANDLHPILDSTKLVANPLAYGAGHVHPNRAAN 621
Query: 601 PGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSGPV 660
PGLVYDL+TNDYLNFLCARGYN+TQ+ KFSNTSFVCS+SFKLTDFNYPSISIP +KS PV
Sbjct: 622 PGLVYDLTTNDYLNFLCARGYNKTQLSKFSNTSFVCSKSFKLTDFNYPSISIPKMKSEPV 681
Query: 661 TIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRGY 720
TI+R VKNVGS TYVARVKVPPGV VSVEP+ LKFTR DEEK FKVV R V K RGY
Sbjct: 682 TIKRTVKNVGSPSTYVARVKVPPGVLVSVEPSTLKFTRTDEEKTFKVVFRSVANNKHRGY 741
Query: 721 VFGSLAWSDGKRHVRSPIAVNLSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSN 780
VFGSL W DGK H SYI YLGSHSHG NPS+ DL++ATESHY+LLGSLLGSN
Sbjct: 742 VFGSLKWLDGKHH---------SYIVYLGSHSHGSNPSSADLRVATESHYSLLGSLLGSN 801
Query: 781 VAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLEN 840
AK+AIFYSYN+YINGFAA+LD VAQ+LA+HP+VVSV ENK RKLHTT SW FL LEN
Sbjct: 802 EMAKEAIFYSYNRYINGFAAVLDHTVAQDLARHPAVVSVQENKMRKLHTTNSWEFLELEN 861
Query: 841 DGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCN 900
G P NSIWN+++FGESTII NLDTGVWPESKSFSD+GYG IPSRWRGSCEGGS F CN
Sbjct: 862 GGGTPPNSIWNVSNFGESTIIANLDTGVWPESKSFSDEGYGAIPSRWRGSCEGGSNFHCN 921
Query: 901 RKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAK 960
RKLIGARYFNKG VA G L+ S++TARD EGHGTHTLSTAGG+FVSGA+VFG GNGTAK
Sbjct: 922 RKLIGARYFNKGVVALAGSLDVSFDTARDQEGHGTHTLSTAGGSFVSGANVFGYGNGTAK 981
Query: 961 GGSPRARVAAYRVCWPAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLA 1020
GGSP+A VAAY+VCW G C ADILA EAAI DGVDVLS+SLG P EF +D A
Sbjct: 982 GGSPKALVAAYKVCWRTFRGGQCSDADILAAIEAAITDGVDVLSLSLGRGPMEFFNDVAA 1041
Query: 1021 IGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKG 1080
IGAFHAVQ GI VVCS GNSGP ++ NVAPW+ TV AST +R F SYV LGN K+I G
Sbjct: 1042 IGAFHAVQQGIVVVCSGGNSGPDPQSIENVAPWLFTVAASTINRQFTSYVALGNEKNITG 1101
Query: 1081 ASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARV 1140
SLSDKILPAQ+FYPLI++ADAKA+NVS+E A+LC EGSLDP+KV+GKI+VC+RGD+ARV
Sbjct: 1102 ESLSDKILPAQQFYPLITSADAKANNVSVEIAKLCVEGSLDPRKVKGKIIVCVRGDDARV 1161
Query: 1141 DKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYM 1200
DKG+VAA+AGAVGMILAN + G++++ADAH+LPASH+SY DGE +++YI STK P+AYM
Sbjct: 1162 DKGFVAAKAGAVGMILANNEKYGNDVVADAHILPASHISYIDGETVYKYINSTKTPVAYM 1221
Query: 1201 THVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKR 1260
THV+TE G+KPAP MASFSSRGP++IEESILKPDI APG++IIAAYSE+ASPSGS FD R
Sbjct: 1222 THVRTETGIKPAPVMASFSSRGPSSIEESILKPDITAPGINIIAAYSEDASPSGSPFDNR 1281
Query: 1261 RSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQL 1320
R PFN SGTSMSCPH++GIV LLKT YPKWSPAAIKSA+MTTA T+ANDLHPIL+ T+L
Sbjct: 1282 RIPFNVVSGTSMSCPHIAGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKL 1341
Query: 1321 KANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFACSHSFK 1380
ANPL+YGAGHV+PN+A NPGLVYDLT DYLNFLCARGYN+ Q+ KFS+ F CS SFK
Sbjct: 1342 VANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFK 1401
Query: 1381 LTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGE 1440
LTDFNYPSISIPN+K GP+ KR VKNVGSP TYVAQV+ PPGV VSVEPN LKFT E
Sbjct: 1402 LTDFNYPSISIPNMKSGPMTIKRTVKNVGSPSTYVAQVEVPPGVLVSVEPNTLKFTRTDE 1461
Query: 1441 EQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 1481
E++F+VV R V N + RGYVFG L W DG H VRS I VNLG
Sbjct: 1462 EKTFKVVFRSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNLG 1493
BLAST of MC00g0428 vs. NCBI nr
Match:
XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])
HSP 1 Score: 2052 bits (5317), Expect = 0.0
Identity = 1044/1562 (66.84%), Postives = 1220/1562 (78.10%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGS-SPSAVD--LELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGF 60
+SYIVYLGS S PS++ T HY+LLG+LLGS + +EAIFYSY +GF
Sbjct: 28 KSYIVYLGSERSSSLDPSSLSEHSRQVTAFHYDLLGTLLGSQKMTEEAIFYSYTTSFNGF 87
Query: 61 AAMIEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGES 120
AA ++EK A +LA+NP V SV ENKARKLHTT SWNFLG+E+D GIPSNSIWN A FG+
Sbjct: 88 AAKLDEKEAANLARNPKVISVFENKARKLHTTRSWNFLGVENDVGIPSNSIWNAAKFGQD 147
Query: 121 TIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAG 180
II N+DTGVWPESKSFSD+GYGP+PS+WRG C+ S FHCNRKLIG RYF KGY A G
Sbjct: 148 IIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLIGGRYFYKGYVAAGG 207
Query: 181 PLNASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQV 240
LNA+ T RDHDGHGTHTLSTA GNFV+GA+VFG+G+GTAKGG+P+ARV AYKVCWP
Sbjct: 208 TLNATSLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGSGTAKGGAPKARVAAYKVCWPP- 267
Query: 241 FSGGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSA 300
F G +C DADILAA EAA++DGVDV+S SLGG + ++ +D AI AFHAVQ GV+VV SA
Sbjct: 268 FLGSQCLDADILAAFEAAVADGVDVISTSLGGAADEYFNDPLAIAAFHAVQQGVVVVFSA 327
Query: 301 GNSGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLI 360
GNSGP P TV N+APW+ TV A T++R F S VALGN++ G SLS +KFYPLI
Sbjct: 328 GNSGPFPMTVTNIAPWINTVAAGTVDRDFASNVALGNKE---GVSLSSIAPLPKKFYPLI 387
Query: 361 SAVDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILA 420
+V+AK NV+ + A+ C EG+LDP KVKGKI++C GE V+K + AA+AGAVG+I+A
Sbjct: 388 DSVNAKLSNVTEFHARFCGEGTLDPMKVKGKIVICQVGEIEGVEKSYQAARAGAVGVIVA 447
Query: 421 NAQENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMAS 480
N E GDE+ + H +PAS ++ D +L+ +Y+ ST PMA++T V+T L IKPAP++A+
Sbjct: 448 NDIEKGDEIYPELHFIPASDITNNDAQLLQKYLNSTTTPMAHLTKVKTLLNIKPAPIIAT 507
Query: 481 FSSRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHI 540
FSSRGPN I+ ILKPDITAPGVNI+A++S P+ SS D RRIP+NV+SGTSMSCPHI
Sbjct: 508 FSSRGPNPIDPFILKPDITAPGVNILASYSTGNAPTFSSNDQRRIPFNVISGTSMSCPHI 567
Query: 541 SGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRA 600
+GI GLLK+++P WSPAAIKSAIMTTA+TR NN I+++ K KA AYGAG V P+ A
Sbjct: 568 AGIAGLLKSIHPDWSPAAIKSAIMTTAKTRGNNLQTILDSTKLKATSYAYGAGMVHPNDA 627
Query: 601 VNPGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSG 660
+PGLVYD + DYLNFLCARGYN +++KF F C +SFK TD NYPSIS+ L+ G
Sbjct: 628 RDPGLVYDTTIEDYLNFLCARGYNAMEMKKFYAKPFNCVKSFKATDLNYPSISVGVLRIG 687
Query: 661 -PVTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKR 720
PVTI RRVK+VGS GTYVARVKV PGV+V VEP L+F+ + EEKAFKVV++ GK KR
Sbjct: 688 APVTINRRVKSVGSPGTYVARVKVSPGVAVLVEPRTLQFSSVGEEKAFKVVLQNTGKVKR 747
Query: 721 RGYVFGSLAWSDGKRHVRSPIAVNL----------------------------------- 780
G VFG+L WSDGK VRSPIAV+L
Sbjct: 748 -GDVFGTLIWSDGKHFVRSPIAVHLGPGSMSLLGAAVMDRWDNLSGIGVVSRWIPPPTLR 807
Query: 781 ----------------------------------------SYIFYLGSHSHGLNPSAIDL 840
SYI YLGSHSHG NPS++DL
Sbjct: 808 LSCDGGGGIGLAMEAFNLSSLLLLFFLFPLLQTSTIATQKSYIVYLGSHSHGSNPSSVDL 867
Query: 841 QLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHEN 900
Q+ATESHY+LLGSLLGSN AAK+AIFYSYN++INGFAA+LD KVA++LA++P+V SVHEN
Sbjct: 868 QIATESHYSLLGSLLGSNEAAKEAIFYSYNRHINGFAAMLDHKVAEDLARNPAVASVHEN 927
Query: 901 KARKLHTTGSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGP 960
K RKLHTT SW FLG+E+D IP+ SIWNLASFGESTII NLDTGVWPESKSFSD+GYGP
Sbjct: 928 KGRKLHTTSSWKFLGVEHDDGIPTKSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGP 987
Query: 961 IPSRWRGSCEGGSKFRCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAG 1020
+P+RW+GSCEGGSKF CNRKLIGARYFNKGY A +G LNA+YETARD +GHGTHTLSTAG
Sbjct: 988 VPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAYVGSLNATYETARDHDGHGTHTLSTAG 1047
Query: 1021 GNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPAVLTGG-CFMADILAGFEAAIHDGVD 1080
GNF+SGA+VFGNGNGTAKGGSP+A VAAY+VCWP V +GG CF ADILA EAAI DGVD
Sbjct: 1048 GNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPPVDSGGSCFDADILAAIEAAISDGVD 1107
Query: 1081 VLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGAST 1140
VLS+SLGG ++FSDD AIGAFHAVQ GI VVCSAGNSGP GTV NVAPW+ TVGAST
Sbjct: 1108 VLSLSLGGDSKDFSDDVAAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIFTVGAST 1167
Query: 1141 ADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLD 1200
+R F SYV LGN+KHIKGASLSDKILPAQKFYPLIS A AKA++VS + AQLCEEGSLD
Sbjct: 1168 INREFTSYVALGNKKHIKGASLSDKILPAQKFYPLISAAYAKANHVSFDDAQLCEEGSLD 1227
Query: 1201 PKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYA 1260
P+KV+GKI++CLRG+NARVDKGYVAAQAGAVGMILAN + N DEL+ADAHLLP SHVSY
Sbjct: 1228 PRKVKGKIIICLRGENARVDKGYVAAQAGAVGMILANTEQNEDELIADAHLLPVSHVSYT 1287
Query: 1261 DGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVS 1320
DG+ I+QYI STK PMAYMTH +TELG+KPAP MASFSSRGPNT+EESILKPDI APGV+
Sbjct: 1288 DGQSIYQYINSTKTPMAYMTHARTELGIKPAPVMASFSSRGPNTMEESILKPDITAPGVN 1347
Query: 1321 IIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALM 1380
I+AAYSE+ASPSGSSFD RR PFN SGTSMSCPH+SGIVGLLKT YPKWSPAAIKSA+M
Sbjct: 1348 ILAAYSEDASPSGSSFDNRRIPFNIVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIM 1407
Query: 1381 TTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYN 1440
TTA T+AND HPILNT +LKANP +YGAGHV+PN+A+NPGLVYDLT KDYLNFLC GYN
Sbjct: 1408 TTAETRANDKHPILNTEELKANPFAYGAGHVQPNRAMNPGLVYDLTTKDYLNFLCVLGYN 1467
Query: 1441 QTQIKKFSSAPFACSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAP 1481
+TQI KFS+ F CS SFKLTDFNYPSISIPN+K G V KRRVKNVG P TYVA+VK P
Sbjct: 1468 KTQISKFSNTSFVCSKSFKLTDFNYPSISIPNMKPGLVTIKRRVKNVGKPSTYVARVKVP 1527
BLAST of MC00g0428 vs. NCBI nr
Match:
XP_008449184.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])
HSP 1 Score: 1954 bits (5062), Expect = 0.0
Identity = 956/1486 (64.33%), Postives = 1173/1486 (78.94%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAM 60
+SYIVYLG+HS G +P+ D+E ATESHY+LLGS++G+N AK++I YSYN++I+GFAA+
Sbjct: 27 KSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAV 86
Query: 61 IEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTII 120
++E+ A DLAKNP V SV ENK R+LHTT SW+FLG+E+D GIPSNSIW A FGE TII
Sbjct: 87 LDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTII 146
Query: 121 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLN 180
GNLD+GVWPESKSF+D GYGP+PSRWRG+CEGG+ F CNRKLIGARYF+ G+ +G +N
Sbjct: 147 GNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLIN 206
Query: 181 ASY-ETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFS 240
S+ +TARD DGHG+HTLSTAGGNFV GAS+FG GNGTAKGGSP+ARV AY+VCWP
Sbjct: 207 VSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALF 266
Query: 241 GGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGN 300
GG C+DADILA EAAISDGVDVLSVSLG ++++FS D +IGAFHAV+ G++VVCSAGN
Sbjct: 267 GGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGN 326
Query: 301 SGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISA 360
SGP P TV N++PW+ TVGASTI+R FTSY LGN+K KG+SLS L KFYPLI+A
Sbjct: 327 SGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINA 386
Query: 361 VDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANA 420
VDA+ N + AQ C+ GSLDP KVKGKI+VCLRG RV+KG+V QAG VGMIL N
Sbjct: 387 VDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVND 446
Query: 421 QENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFS 480
+ +G L++D+H+LPA+ ++Y DG V QYI ST P+A +T V T+LG+KP+P+MA FS
Sbjct: 447 KTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFS 506
Query: 481 SRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISG 540
SRGPN I +++LKPDIT PGVNI+A+ + D + FD RR+P+NV SGTSMSCPHI+G
Sbjct: 507 SRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAG 566
Query: 541 IVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVN 600
+ GLLKTLYP WSPAAIKSAIMTTA+TR N+ H + +T+K KA P YGAGHV P+ A++
Sbjct: 567 VSGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMD 626
Query: 601 PGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSG-P 660
PGLVYD + +DYLNFLCARGYN + F + FVC+ F LTD NYPSISIP LKSG P
Sbjct: 627 PGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAP 686
Query: 661 VTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRG 720
VT+ RRVKNVG+ GTYVARVK +SV+VEP+ L+F + EEKAFKV+ G G+ +
Sbjct: 687 VTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVLFEYKGTGQGKS 746
Query: 721 YVFGSLAWSD-GKRHVRSPIAVNL-SYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLL 780
YVFG+L WSD GK +VRSPI V L SYI YLGSH G NPS D+QLATES Y LL S++
Sbjct: 747 YVFGTLIWSDNGKHNVRSPIVVKLGSYIVYLGSHFFGSNPSIHDVQLATESQYELLESVV 806
Query: 781 GSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLG 840
GS +AAK++IFYSYN+YINGFAAILDE A LA++P+VVS+ EN+ RKLHTT SWSFLG
Sbjct: 807 GSKLAAKESIFYSYNRYINGFAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLG 866
Query: 841 LENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKF 900
+E+D IP NSIW A FGE TIIGNLDTG WPESKSF+D GYGP+PSRW G CEGG+ F
Sbjct: 867 MESDEGIPPNSIWKAARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANF 926
Query: 901 RCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNG 960
CN+KLIGARYFNKG+ A GP++A+ TARD EGHG+HTLSTAGGNFV GA+VFGNGNG
Sbjct: 927 TCNKKLIGARYFNKGFEAENGPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNG 986
Query: 961 TAKGGSPRARVAAYRVCWPAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDD 1020
TAKGGSPRAR+AAY+VCWP+ TGGC+ ADILA E+AIHDGVDVLS+SLG S +F+ D
Sbjct: 987 TAKGGSPRARLAAYKVCWPS-FTGGCYDADILAAVESAIHDGVDVLSISLGSSARDFASD 1046
Query: 1021 GLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKH 1080
L+IGAFHAVQ GI VVCS GN GP GTV+NV+PWMITV AST DR F +YV LGN++H
Sbjct: 1047 TLSIGAFHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRH 1106
Query: 1081 IKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDN 1140
KG SLS LP KFYPL+ KA N + + A LCE+GSLDP K +GKIV+CLRGD+
Sbjct: 1107 FKGVSLSSGGLPRGKFYPLVDGVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDS 1166
Query: 1141 ARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPM 1200
AR+DK + +AG +G+IL N +++G+++ AD H LPASH++YADG IFQYI STK PM
Sbjct: 1167 ARMDKSFEVRRAGGIGLILVNDKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPM 1226
Query: 1201 AYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSF 1260
A++THVKTE+G+KP+P +A FSSRGPN I +S++KPDI APGVSI+AA+SE A+ +
Sbjct: 1227 AFITHVKTEMGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPL 1286
Query: 1261 DKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNT 1320
D RR FN ESGTSM+CPH+SG+VGLLKT YPKWSPAAIKSA+MTTA T+ N + IL+
Sbjct: 1287 DTRRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDY 1346
Query: 1321 TQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFACSH 1380
+ KA P YGAGHV PN A++PGLVYD T++DY+NF+CA+GYN T +K+F + P+ C
Sbjct: 1347 NKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPK 1406
Query: 1381 SFKLTDFNYPSISIPNLKFG-PVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFT 1440
SF LTD NYPSIS+P L G PV RR+KNVG+PGTYVA+VK V+V+V+P+ L+F
Sbjct: 1407 SFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFN 1466
Query: 1441 GIGEEQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 1481
+GEE++F+VV + +G+VFG L WSDGNH VRSP+AV LG
Sbjct: 1467 SVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGNHFVRSPMAVKLG 1511
BLAST of MC00g0428 vs. ExPASy TrEMBL
Match:
A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)
HSP 1 Score: 2382 bits (6173), Expect = 0.0
Identity = 1180/1499 (78.72%), Postives = 1322/1499 (88.19%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAM 60
QSYIVYLGSHSHGS+PSA DLE+ATESHY+LLGSLLGSNEAAKEAIFYSYNRHI+GFAA+
Sbjct: 6 QSYIVYLGSHSHGSNPSAADLEIATESHYSLLGSLLGSNEAAKEAIFYSYNRHINGFAAI 65
Query: 61 IEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTII 120
+++KV EDLA+NPAVAS+HENK RKLHTTSSW FLG+EHD GIP NSIWN ASFGESTII
Sbjct: 66 LDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEHDDGIPHNSIWNRASFGESTII 125
Query: 121 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLN 180
GNLDTGVWPESKSF+D+GYGP+P+RW+GSCEGGSKFHCNRKLIGARYFNKGYAAYAG LN
Sbjct: 126 GNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCNRKLIGARYFNKGYAAYAGSLN 185
Query: 181 ASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFSG 240
ASYETARD++GHGTHTLSTAGGNF+SGA+VFGNGNGTAKGGSP+A V AYKVCWPQV SG
Sbjct: 186 ASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTAKGGSPKALVAAYKVCWPQVDSG 245
Query: 241 GECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGNS 300
G CFDADILAAIEAAISDGVD+LS+SLGG +KDFS+DVTAIGAFHAVQ G+IVVCSAGNS
Sbjct: 246 GGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDVTAIGAFHAVQQGIIVVCSAGNS 305
Query: 301 GPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISAV 360
GPAPGT++N APW++TVGASTINR FTSYVALGN+KHIKG SLSDKILP QKFYPLI+A
Sbjct: 306 GPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHIKGASLSDKILPEQKFYPLINAA 365
Query: 361 DAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANAQ 420
DAKA NVS+ AQ+C GSLDPKKVKGKII+CLRGEN RVDKG+ AAQAGAVGMILANA+
Sbjct: 366 DAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENARVDKGYAAAQAGAVGMILANAE 425
Query: 421 ENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFSS 480
+NGDEL+ADAHLLP SHVSY DG+ ++QYI TK PMAYMTHVRTELGIKPAP+MASFSS
Sbjct: 426 QNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMAYMTHVRTELGIKPAPVMASFSS 485
Query: 481 RGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISGI 540
RGPNTIEESILKPDITAPGVNI+AA+SEDA PSGS FDNRRIP+N+VSGTSMSCPHISGI
Sbjct: 486 RGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFDNRRIPFNIVSGTSMSCPHISGI 545
Query: 541 VGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVNP 600
VGLLKTLYP WSPAAIKSAIMTTAETRAN+ HPI+NT+ KANP AYGAGHV+P+RA+NP
Sbjct: 546 VGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTVNLKANPFAYGAGHVQPNRAMNP 605
Query: 601 GLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSGPVT 660
GLVYDL+TNDY+NFLCA+GYN++QI KFS TSFVCS+SFKLTDFNYPSISIP++KSG VT
Sbjct: 606 GLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKSFKLTDFNYPSISIPDMKSGVVT 665
Query: 661 IRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRGYV 720
I RRVKNVG TYVARVKVP GVSVSVEP LKFT IDEEK+FKVV+ V K RGYV
Sbjct: 666 INRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGIDEEKSFKVVIGSVANNKHRGYV 725
Query: 721 FGSLAWSDGKRHVRSPIAVNL-SYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLG-- 780
FGSL W DGK HVRSPI VNL SYI YLGSHSHGLNPS+ID Q+ATESHYNLLGSLLG
Sbjct: 726 FGSLIWEDGKHHVRSPIVVNLGSYIVYLGSHSHGLNPSSIDAQIATESHYNLLGSLLGRY 785
Query: 781 ----------SNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLH 840
SN AK+AIFYSYN++INGFAA++D+KVA++LAKHP VVSV ENK RKLH
Sbjct: 786 LHSVCCVSKYSNEEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLH 845
Query: 841 TTGSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWR 900
TT SWSFLG+E++G IPSNSIWNLASFGESTIIGNLDTGVWPE+KSF DK YGPIPSRW+
Sbjct: 846 TTNSWSFLGVESNGVIPSNSIWNLASFGESTIIGNLDTGVWPEAKSFDDKEYGPIPSRWK 905
Query: 901 GSCEGGSKFRCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSG 960
GSCEGGS F+CNRKLIGARY+NKGY GPLN+SYE+ARD EGHGTHTLSTAGGNFV
Sbjct: 906 GSCEGGSNFQCNRKLIGARYYNKGYAEIAGPLNSSYESARDHEGHGTHTLSTAGGNFVPN 965
Query: 961 ASVFGNGNGTAKGGSPRARVAAYRVCWPAV-LTGGCFMADILAGFEAAIHDGVDVLSVSL 1020
A++FG GNGTAKGGSP+A VAAY+VCWP + L G CF ADILAGFEAAI DGVDVLSVSL
Sbjct: 966 ANLFGYGNGTAKGGSPKALVAAYKVCWPKLWLFGECFDADILAGFEAAIADGVDVLSVSL 1025
Query: 1021 GGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFA 1080
GG P +F D +AIG+FHAVQ+GITVVCSAGNSGP GTVSNVAPW+ITVGASTADRL+
Sbjct: 1026 GGDPSDFGQDSIAIGSFHAVQNGITVVCSAGNSGPAPGTVSNVAPWIITVGASTADRLYT 1085
Query: 1081 SYVGLGNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEG 1140
+YV +G+++H KGASLS+K LP QKFYPLI++ DAK N S +YAQLCE SLDP+KV+G
Sbjct: 1086 NYVAIGDKRHFKGASLSEKGLPVQKFYPLINSVDAKVQNFSNKYAQLCEIESLDPEKVKG 1145
Query: 1141 KIVVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIF 1200
KIVVCLRGDNAR +KGYV A+AG VGMILANA++NGD++ ADAHLLPASH++Y+DG+L++
Sbjct: 1146 KIVVCLRGDNARTEKGYVVAKAGGVGMILANAEENGDDISADAHLLPASHITYSDGQLVY 1205
Query: 1201 QYIKSTKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYS 1260
QYI STKIPMAY+TH +TE GVKPAP MASFSSRGPN+I+ SILKPDI APG +I+AAYS
Sbjct: 1206 QYINSTKIPMAYLTHPRTESGVKPAPIMASFSSRGPNSIDPSILKPDITAPGENILAAYS 1265
Query: 1261 EEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATK 1320
E+ SPSG FDKRR PFN ESGTSMSCPHVSGIVGLLKT YPKWSPAAI+SA+MTTA TK
Sbjct: 1266 EDGSPSGLYFDKRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTK 1325
Query: 1321 ANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKK 1380
ANDL+PIL+T Q KAN +YGAGHVRPNKA +PGLVYDL+ +DYLN+LCA GY QIK+
Sbjct: 1326 ANDLNPILSTKQEKANEWAYGAGHVRPNKAADPGLVYDLSTEDYLNYLCALGYKIKQIKQ 1385
Query: 1381 FSS-APFACSHSFKLTDFNYPSISIPNLKFG-PVKAKRRVKNVGSPGTYVAQVKAPPGVA 1440
FS+ F CS SFK+TD NYPSISIPNL+ +K KRR+KNVGSPGTYV QV AP GV+
Sbjct: 1386 FSNDTSFVCSKSFKITDLNYPSISIPNLESDVALKIKRRLKNVGSPGTYVVQVNAPLGVS 1445
Query: 1441 VSVEPNMLKFTGIGEEQSFRVVVR-RVENEERRGYVFGWLAWSDGN-HRVRSPIAVNLG 1481
VSVEP LKFTGI EE+SFRVV++ V N YVFG L WSDGN HRVRSPI V LG
Sbjct: 1446 VSVEPTSLKFTGIDEEKSFRVVLKSNVPNGFHPEYVFGKLEWSDGNNHRVRSPIVVKLG 1504
BLAST of MC00g0428 vs. ExPASy TrEMBL
Match:
A0A1S3BLG8 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=1)
HSP 1 Score: 1954 bits (5062), Expect = 0.0
Identity = 956/1486 (64.33%), Postives = 1173/1486 (78.94%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAM 60
+SYIVYLG+HS G +P+ D+E ATESHY+LLGS++G+N AK++I YSYN++I+GFAA+
Sbjct: 27 KSYIVYLGAHSFGPNPTNFDIEAATESHYDLLGSVVGNNLEAKDSIKYSYNKYINGFAAV 86
Query: 61 IEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTII 120
++E+ A DLAKNP V SV ENK R+LHTT SW+FLG+E+D GIPSNSIW A FGE TII
Sbjct: 87 LDEQQATDLAKNPKVVSVFENKKRQLHTTRSWSFLGMENDEGIPSNSIWKAARFGEDTII 146
Query: 121 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLN 180
GNLD+GVWPESKSF+D GYGP+PSRWRG+CEGG+ F CNRKLIGARYF+ G+ +G +N
Sbjct: 147 GNLDSGVWPESKSFNDAGYGPVPSRWRGACEGGNNFQCNRKLIGARYFHAGFVMGSGLIN 206
Query: 181 ASY-ETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFS 240
S+ +TARD DGHG+HTLSTAGGNFV GAS+FG GNGTAKGGSP+ARV AY+VCWP
Sbjct: 207 VSFNKTARDEDGHGSHTLSTAGGNFVPGASIFGYGNGTAKGGSPKARVAAYRVCWPPALF 266
Query: 241 GGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGN 300
GG C+DADILA EAAISDGVDVLSVSLG ++++FS D +IGAFHAV+ G++VVCSAGN
Sbjct: 267 GGGCYDADILAGFEAAISDGVDVLSVSLGSEAQEFSHDSMSIGAFHAVEQGIVVVCSAGN 326
Query: 301 SGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISA 360
SGP P TV N++PW+ TVGASTI+R FTSY LGN+K KG+SLS L KFYPLI+A
Sbjct: 327 SGPGPKTVSNVSPWMFTVGASTIDRDFTSYATLGNKKQYKGSSLSSSSLAGGKFYPLINA 386
Query: 361 VDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANA 420
VDA+ N + AQ C+ GSLDP KVKGKI+VCLRG RV+KG+V QAG VGMIL N
Sbjct: 387 VDARNPNSTDSFAQKCETGSLDPAKVKGKIVVCLRGVTARVEKGYVVLQAGGVGMILVND 446
Query: 421 QENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFS 480
+ +G L++D+H+LPA+ ++Y DG V QYI ST P+A +T V T+LG+KP+P+MA FS
Sbjct: 447 KTDGSGLISDSHILPATSLTYTDGLAVAQYINSTTTPVALITPVETQLGVKPSPVMAGFS 506
Query: 481 SRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISG 540
SRGPN I +++LKPDIT PGVNI+A+ + D + FD RR+P+NV SGTSMSCPHI+G
Sbjct: 507 SRGPNPITDAMLKPDITGPGVNILASVTTDVTATKFPFDTRRVPFNVESGTSMSCPHIAG 566
Query: 541 IVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVN 600
+ GLLKTLYP WSPAAIKSAIMTTA+TR N+ H + +T+K KA P YGAGHV P+ A++
Sbjct: 567 VSGLLKTLYPTWSPAAIKSAIMTTAKTRDNSMHTLSDTVKAKATPFDYGAGHVNPNNAMD 626
Query: 601 PGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSG-P 660
PGLVYD + +DYLNFLCARGYN + F + FVC+ F LTD NYPSISIP LKSG P
Sbjct: 627 PGLVYDTTIDDYLNFLCARGYNAQTLMSFHSKPFVCATPFTLTDLNYPSISIPELKSGAP 686
Query: 661 VTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRG 720
VT+ RRVKNVG+ GTYVARVK +SV+VEP+ L+F + EEKAFKV+ G G+ +
Sbjct: 687 VTVNRRVKNVGTPGTYVARVKASSKISVTVEPSTLQFNSVGEEKAFKVLFEYKGTGQGKS 746
Query: 721 YVFGSLAWSD-GKRHVRSPIAVNL-SYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLL 780
YVFG+L WSD GK +VRSPI V L SYI YLGSH G NPS D+QLATES Y LL S++
Sbjct: 747 YVFGTLIWSDNGKHNVRSPIVVKLGSYIVYLGSHFFGSNPSIHDVQLATESQYELLESVV 806
Query: 781 GSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLG 840
GS +AAK++IFYSYN+YINGFAAILDE A LA++P+VVS+ EN+ RKLHTT SWSFLG
Sbjct: 807 GSKLAAKESIFYSYNRYINGFAAILDENQAIALARNPNVVSIFENQKRKLHTTRSWSFLG 866
Query: 841 LENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKF 900
+E+D IP NSIW A FGE TIIGNLDTG WPESKSF+D GYGP+PSRW G CEGG+ F
Sbjct: 867 MESDEGIPPNSIWKAARFGEDTIIGNLDTGAWPESKSFNDAGYGPVPSRWMGVCEGGANF 926
Query: 901 RCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNG 960
CN+KLIGARYFNKG+ A GP++A+ TARD EGHG+HTLSTAGGNFV GA+VFGNGNG
Sbjct: 927 TCNKKLIGARYFNKGFEAENGPMSANLTTARDQEGHGSHTLSTAGGNFVPGANVFGNGNG 986
Query: 961 TAKGGSPRARVAAYRVCWPAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDD 1020
TAKGGSPRAR+AAY+VCWP+ TGGC+ ADILA E+AIHDGVDVLS+SLG S +F+ D
Sbjct: 987 TAKGGSPRARLAAYKVCWPS-FTGGCYDADILAAVESAIHDGVDVLSISLGSSARDFASD 1046
Query: 1021 GLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKH 1080
L+IGAFHAVQ GI VVCS GN GP GTV+NV+PWMITV AST DR F +YV LGN++H
Sbjct: 1047 TLSIGAFHAVQQGIVVVCSGGNDGPTPGTVTNVSPWMITVAASTVDRDFVNYVALGNKRH 1106
Query: 1081 IKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDN 1140
KG SLS LP KFYPL+ KA N + + A LCE+GSLDP K +GKIV+CLRGD+
Sbjct: 1107 FKGVSLSSGGLPRGKFYPLVDGVQVKAGNATDKLALLCEDGSLDPAKAKGKIVLCLRGDS 1166
Query: 1141 ARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPM 1200
AR+DK + +AG +G+IL N +++G+++ AD H LPASH++YADG IFQYI STK PM
Sbjct: 1167 ARMDKSFEVRRAGGIGLILVNDKEDGNDITADPHFLPASHLNYADGIAIFQYINSTKSPM 1226
Query: 1201 AYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSF 1260
A++THVKTE+G+KP+P +A FSSRGPN I +S++KPDI APGVSI+AA+SE A+ +
Sbjct: 1227 AFITHVKTEMGIKPSPMVADFSSRGPNPIIDSMIKPDIAAPGVSILAAFSEYATATDFPL 1286
Query: 1261 DKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNT 1320
D RR FN ESGTSM+CPH+SG+VGLLKT YPKWSPAAIKSA+MTTA T+ N + IL+
Sbjct: 1287 DTRRVSFNFESGTSMACPHISGVVGLLKTLYPKWSPAAIKSAIMTTAKTRDNSMKSILDY 1346
Query: 1321 TQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFACSH 1380
+ KA P YGAGHV PN A++PGLVYD T++DY+NF+CA+GYN T +K+F + P+ C
Sbjct: 1347 NKAKATPFQYGAGHVHPNNAIDPGLVYDTTIEDYMNFICAQGYNSTTLKRFYNKPYLCPK 1406
Query: 1381 SFKLTDFNYPSISIPNLKFG-PVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFT 1440
SF LTD NYPSIS+P L G PV RR+KNVG+PGTYVA+VK V+V+V+P+ L+F
Sbjct: 1407 SFPLTDLNYPSISVPKLTIGVPVTINRRLKNVGTPGTYVARVKVSSKVSVTVKPSTLQFN 1466
Query: 1441 GIGEEQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 1481
+GEE++F+VV + +G+VFG L WSDGNH VRSP+AV LG
Sbjct: 1467 SVGEEKAFKVVFEYKGKGQGKGHVFGTLIWSDGNHFVRSPMAVKLG 1511
BLAST of MC00g0428 vs. ExPASy TrEMBL
Match:
A0A6J1KUL8 (uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820 PE=3 SV=1)
HSP 1 Score: 1929 bits (4997), Expect = 0.0
Identity = 956/1518 (62.98%), Postives = 1168/1518 (76.94%), Query Frame = 0
Query: 1 QSYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAM 60
+SYIVYLGS S +PS D++LATES Y++LG++ GS AAKE+I Y+YNR I+GFAA+
Sbjct: 26 KSYIVYLGSES---NPSIYDVQLATESKYDILGTVKGSKVAAKESILYTYNRCINGFAAV 85
Query: 61 IEEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTII 120
+++K LAKNP+V S+ EN+ RKLHTT SW+FLG+ D GIP NSIW + FGE II
Sbjct: 86 LDDKEVTALAKNPSVVSIFENRERKLHTTRSWSFLGVGSDRGIPWNSIWKASKFGEDVII 145
Query: 121 GNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFHCNRKLIGARYFNKGYAAYAGPLN 180
GNLDTGVWPES SF D GYGP+PSRW G+CEGGS F CNRKLIGARYF +GY GPLN
Sbjct: 146 GNLDTGVWPESHSFHDSGYGPVPSRWMGACEGGSNFSCNRKLIGARYFYRGYEMINGPLN 205
Query: 181 ASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFSG 240
S ARDH+GHGTHTLSTAGGNFV GA+VFGNGNGTAKGG+P+ARV AYKVCWPQ
Sbjct: 206 ISSLNARDHEGHGTHTLSTAGGNFVRGANVFGNGNGTAKGGAPKARVAAYKVCWPQ---- 265
Query: 241 GECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGNS 300
G+C DAD+LA IEAAISDGVDVLS+SLG ++DF+DD ++GAFHA+Q G+IVVCSAGN
Sbjct: 266 GKCSDADLLAGIEAAISDGVDVLSISLGAAAQDFADDPISVGAFHAIQQGIIVVCSAGND 325
Query: 301 GPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISAV 360
GP PGTV N++PW+ TVGAS+I+R F SYV LGN+K +KG+SLS LP K YPL+++V
Sbjct: 326 GPLPGTVTNVSPWMFTVGASSIDRGFFSYVYLGNKKQVKGSSLSSGGLPRGKLYPLMNSV 385
Query: 361 DAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANAQ 420
+AKA N S AQ+C+EGSLDP K +GKIIVCLRG+NGR+DK + G VGMIL N +
Sbjct: 386 NAKASNASDGLAQLCEEGSLDPMKARGKIIVCLRGDNGRMDKSFEVLRVGGVGMILVNDK 445
Query: 421 ENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFSS 480
+G ++ DAH+LP SHVSY DG + +Y+KSTK P+A +T VRTE+GIKP+P+MA FSS
Sbjct: 446 ISGSDIETDAHMLPTSHVSYIDGLSIAEYLKSTKRPVASITPVRTEIGIKPSPVMAPFSS 505
Query: 481 RGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISGI 540
RGPN I E+++KPDI+APGVNIIA+F++ + FD RR+P+NV SGTSMSCPHI+G+
Sbjct: 506 RGPNHIAEAMIKPDISAPGVNIIASFTKAVAATDLPFDKRRVPFNVQSGTSMSCPHIAGV 565
Query: 541 VGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVNP 600
GLLK L+P WSPAAIKSAIMTTA+TR N + +++ K KA P YGAGHV P+ A++P
Sbjct: 566 AGLLKKLHPTWSPAAIKSAIMTTAKTRDNTKNTMLDFNKVKATPFDYGAGHVHPNDAMDP 625
Query: 601 GLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSG-PV 660
GLVYD + +DYLNFLC +GYN ++KFSN FVC+++F TD NYPSIS+P L+ G PV
Sbjct: 626 GLVYDTTIDDYLNFLCMQGYNSLTLKKFSNKPFVCAKNFASTDLNYPSISVPKLQIGVPV 685
Query: 661 TIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRGY 720
T+ RRVKNVGSAGTYVARV++P G++V VEP+ L+F + EEKAFK+V K +R GY
Sbjct: 686 TVNRRVKNVGSAGTYVARVRMPKGITVMVEPSTLQFHSVGEEKAFKLVFHYAQKVRRPGY 745
Query: 721 VFGSLAWSDGKRHVRSPIAVNL---------------------------------SYIFY 780
VFG+L WSDGK VRSPIAVNL SYI Y
Sbjct: 746 VFGALVWSDGKHFVRSPIAVNLVNFKAKAMEEYNKISPLLLFFFMLHTAAVPTKNSYIVY 805
Query: 781 LGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAILDEKVA 840
LGSHS NPS D+QLATES Y++LGS+ GS VAAKD+I YSYN+YINGFAA+LDE+ A
Sbjct: 806 LGSHSFAPNPSVYDVQLATESQYDILGSVKGSKVAAKDSILYSYNRYINGFAAVLDEQEA 865
Query: 841 QELAKHPSVVSVHENKARKLHTTGSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTG 900
LAK+PSVVSV ENK RKLHTT SW FLG+++D IP NSIW A FG TIIGNLDTG
Sbjct: 866 TALAKNPSVVSVFENKERKLHTTRSWGFLGVDSDRGIPQNSIWKAARFGADTIIGNLDTG 925
Query: 901 VWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCNRKLIGARYFNKGYVASIGPL---NASY 960
VWPES SF+D GYGP+PSRWRG+CEGGSKFRCNRKLIGARYF +G+ A+ GPL N S+
Sbjct: 926 VWPESPSFNDAGYGPVPSRWRGACEGGSKFRCNRKLIGARYFYRGFQAAEGPLTTHNISF 985
Query: 961 ETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPAVLTGGCF 1020
++ARD EGHG+HTLSTAGGNFV G +VFGNGNGTAKGGSPRARV AY+VCWP+ GGC+
Sbjct: 986 DSARDHEGHGSHTLSTAGGNFVHGVNVFGNGNGTAKGGSPRARVVAYKVCWPSK-NGGCY 1045
Query: 1021 MADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGR 1080
+DILAG EAAI DGVDVLS S+G +EF++D ++IGAFHAVQHGI VVCSAGN GP
Sbjct: 1046 DSDILAGIEAAISDGVDVLSASIGTLAQEFANDAISIGAFHAVQHGIVVVCSAGNDGPSP 1105
Query: 1081 GTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLISTADAKA 1140
G+VSNV+PWM+TVGAST DR F SYV LGN+K +G+SLS LPA KFYPLI KA
Sbjct: 1106 GSVSNVSPWMVTVGASTIDRDFVSYVVLGNKKRFRGSSLSSSRLPAGKFYPLIKAVQVKA 1165
Query: 1141 SNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGD 1200
+N + +AQLC +G+LDP K +GKI+VCLRG+NARV KG+ + G VGM+L N Q +G
Sbjct: 1166 ANATDGFAQLCMDGTLDPTKAKGKIIVCLRGENARVSKGFEVFRVGGVGMVLVNNQMDGS 1225
Query: 1201 ELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMASFSSRGPN 1260
L+AD H+LPASH+SYADG I QY+ STK P+A +TH TE+G+KP+P MASFSSRGP+
Sbjct: 1226 ALVADPHILPASHLSYADGVSIAQYLSSTKTPVASITHASTEMGIKPSPLMASFSSRGPD 1285
Query: 1261 TIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLL 1320
I ++++KPDI APGV+IIA+ +++ S SG DKRR PFN ESGTSMSCPH+SG+ GLL
Sbjct: 1286 FITQAVIKPDITAPGVNIIASVTDDISASGLPLDKRRVPFNIESGTSMSCPHISGVAGLL 1345
Query: 1321 KTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKALNPGLVY 1380
KT +P WSPAAIKSA+MTTA T+ N + +L+ T++KA P YGAGHV PN A++PGLVY
Sbjct: 1346 KTLHPTWSPAAIKSAVMTTAKTRDNTKNTLLDYTKVKATPFDYGAGHVHPNDAMDPGLVY 1405
Query: 1381 DLTLKDYLNFLCARGYNQTQIKKFSSAPFACSHSFKLTDFNYPSISIPNLKFG-PVKAKR 1440
D T+ DYLNFLC RGYN +KKFS+ PF C+++F TDFNYPSI +P L+ G V R
Sbjct: 1406 DTTVDDYLNFLCTRGYNSRTLKKFSNKPFVCANNFATTDFNYPSILVPRLQIGGSVTVNR 1465
Query: 1441 RVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGEEQSFRVVVRRVENEERRGYVFGW 1480
RVKNVGS GTYVA+V+ P G+ V VEP+ L+F +GEE+ F+++ R+GYVFG
Sbjct: 1466 RVKNVGSTGTYVARVRMPKGITVMVEPSKLQFHSVGEERPFKLIFHYAHKLRRQGYVFGA 1525
BLAST of MC00g0428 vs. ExPASy TrEMBL
Match:
A0A6J5V7Q5 (Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS35141 PE=3 SV=1)
HSP 1 Score: 1894 bits (4907), Expect = 0.0
Identity = 941/1488 (63.24%), Postives = 1158/1488 (77.82%), Query Frame = 0
Query: 2 SYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMI 61
SYIVYLG+ H PS+VDL+ T SHYNLLGS+LGSNE A+EAIFYSYNR+I+GFAA++
Sbjct: 29 SYIVYLGAQPHVLDPSSVDLDSVTNSHYNLLGSVLGSNERAQEAIFYSYNRNINGFAAIL 88
Query: 62 EEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIG 121
+++ A +A++P V SV N+ RKLHTT SW+FLGLE +G + SIW A FG +TIIG
Sbjct: 89 DDEEAAQIARDPNVLSVFPNRGRKLHTTRSWDFLGLEENGEVRRGSIWKKAQFGANTIIG 148
Query: 122 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSK--FHCNRKLIGARYFNKGYAAYAGPL 181
NLDTGVWPESKSFSD+G GPIPS+WRG C+ +K HCNRKLIGARYF+KGY AYA +
Sbjct: 149 NLDTGVWPESKSFSDEGIGPIPSKWRGICQLDTKNGSHCNRKLIGARYFSKGYLAYASTV 208
Query: 182 NAS-----YETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCW 241
N+S ARD GHG+HTLSTA GNFV ASVFGNGNGTAKGGSP+ARV AYKVCW
Sbjct: 209 NSSAAKTIQPNARDFGGHGSHTLSTAAGNFVPRASVFGNGNGTAKGGSPKARVAAYKVCW 268
Query: 242 PQVFSGGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVV 301
P + +G ECFDADI+AA +AAISDGVDVLSVSLGG + +F D AIG+FHAV+ G+ VV
Sbjct: 269 PPI-NGNECFDADIMAAFDAAISDGVDVLSVSLGGQAAEFFSDGIAIGSFHAVKKGISVV 328
Query: 302 CSAGNSGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFY 361
SAGNSGP PGTV N++PWL+TVGASTI+R F+SYVALGN+KH+KG SLS LP++ FY
Sbjct: 329 SSAGNSGPTPGTVSNVSPWLLTVGASTIDREFSSYVALGNKKHLKGASLSSGALPSKMFY 388
Query: 362 PLISAVDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGM 421
PLISAVD KA N S+ +AQ+C GSL+ KKV+GKI+VC+RGEN R DKG A AGAVGM
Sbjct: 389 PLISAVDGKAANASSSDAQLCKAGSLEKKKVEGKILVCIRGENARADKGQQAVLAGAVGM 448
Query: 422 ILANAQENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPL 481
IL N + +G+E++AD HLLP SHV+Y+DG+ VF YIKSTK P+AY+T V+TE+G KPAP
Sbjct: 449 ILVNDKLSGNEIIADPHLLPTSHVNYSDGKAVFAYIKSTKTPVAYLTRVKTEVGAKPAPF 508
Query: 482 MASFSSRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSC 541
MASFSSRGPNTIE+SILKPDITAPGV+IIAA++ P+ FD RR+ +N SGTSMSC
Sbjct: 509 MASFSSRGPNTIEQSILKPDITAPGVSIIAAYTGAEGPTDQKFDKRRVSFNTESGTSMSC 568
Query: 542 PHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRP 601
PH+SGIVGLLKTL+P WSPAAIKSAIMTTA R NN + ++ K +A P AYGAGHV+P
Sbjct: 569 PHVSGIVGLLKTLHPSWSPAAIKSAIMTTARKRDNNKEAMQDSSKARATPFAYGAGHVQP 628
Query: 602 SRAVNPGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNL 661
+RA++PGLVYDL+T+DYLNFLCARGYN T ++ FSN C +++ L DFNYPS+++P+L
Sbjct: 629 NRAMDPGLVYDLTTDDYLNFLCARGYNATLLKVFSNEPHTCPKAYSLADFNYPSVTVPDL 688
Query: 662 KSGPVTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKG 721
PV + RRVKNVGS GTYV +K P GVSVSV+P+ ++F I EEK FKVV++ +G
Sbjct: 689 HDKPVAVTRRVKNVGSPGTYVVHIKEPAGVSVSVKPSSMQFKTIGEEKKFKVVLKPKVQG 748
Query: 722 KRRGYVFGSLAWSDGKRHVRSPIAVNLSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGS 781
+ YVFG L WSDGK N S+I YLG+HSHG +PS++DL + HY+ LGS
Sbjct: 749 TQ-DYVFGELNWSDGKH--------NQSFIVYLGAHSHGPDPSSLDLDSVRKFHYDFLGS 808
Query: 782 LLGSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSF 841
L SN +AKD IFYSY ++INGFAAIL+E+ A ++A+HP+V+SV NK KL TT SW+F
Sbjct: 809 FLRSNKSAKDVIFYSYTRHINGFAAILEEEEAADIAEHPNVISVFLNKGSKLETTRSWNF 868
Query: 842 LGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGS 901
LGLE +G IPS+SIW A GE TII N+DTGVWPESKSFSD+G GP+PS+WRG C+ +
Sbjct: 869 LGLERNGLIPSHSIWMKARLGEDTIIANIDTGVWPESKSFSDEGLGPVPSKWRGICQHDT 928
Query: 902 K-FRCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGN 961
K RCNRKLIG RYFN G GPLN+S+ TARD +GHG+HTL+TA GNFV G SVFGN
Sbjct: 929 KRVRCNRKLIGTRYFNNGLAMYAGPLNSSFSTARDYDGHGSHTLATAAGNFVPGVSVFGN 988
Query: 962 GNGTAKGGSPRARVAAYRVCWPAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEF 1021
GNGTAKGGSPRA VAAY+VCWP CF AD+LA F+AAI DGVD++SVSLGG +EF
Sbjct: 989 GNGTAKGGSPRAHVAAYKVCWPPYEGVQCFDADVLAAFDAAISDGVDIISVSLGGGAQEF 1048
Query: 1022 SDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGN 1081
++IGAFHAV+HGI VV +AGN+GP GTV N++PW++TVGA T DR F SYV LGN
Sbjct: 1049 FKSSISIGAFHAVKHGIVVVSAAGNTGPNPGTVLNLSPWLLTVGAGTIDREFTSYVSLGN 1108
Query: 1082 RKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLR 1141
+KH+KG SLS K LP++KFYPL+S A+AK +N S A +C+ G+LDP+KV+GKI+VCLR
Sbjct: 1109 KKHLKGVSLSAKGLPSEKFYPLVSAAEAKHANASTAEAIICQGGTLDPRKVKGKILVCLR 1168
Query: 1142 --GDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKS 1201
DNAR +K + A AGAVGMIL N + +G++++AD H+L SHV+Y DG+ IF YIKS
Sbjct: 1169 EYNDNARTEKSWQADMAGAVGMILVNDEQSGNDVVADPHVLLVSHVNYTDGKYIFDYIKS 1228
Query: 1202 TKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASP 1261
TK PMAY+T VKTELG KPAPF+A+FSSRGPN E+ ILKPDIIAPGVSIIAAY+E A P
Sbjct: 1229 TKTPMAYLTRVKTELGSKPAPFVATFSSRGPNLQEQGILKPDIIAPGVSIIAAYTEAAGP 1288
Query: 1262 SGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLH 1321
+ D RR PFN ++G+SM+CPH SGI GLL+T +P WSPAAIKSA+MTTA T+ + +
Sbjct: 1289 TSQISDTRRVPFNVQTGSSMACPHASGIAGLLRTLHPDWSPAAIKSAIMTTATTQDDHME 1348
Query: 1322 PILN-TTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSA 1381
PIL+ ++ +KA P +YG+GH++PNKA++PGLVY+LT DYLNFLCARGYN+T IK FS++
Sbjct: 1349 PILDDSSYVKATPFAYGSGHIQPNKAMDPGLVYNLTTLDYLNFLCARGYNETMIKSFSNS 1408
Query: 1382 PFACSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPN 1441
+ CS SF L DFNYPSIS+PNL V R+V NVGSPGTY VK P V V VEP
Sbjct: 1409 TYKCSKSFSLADFNYPSISVPNLSEDSVTINRKVTNVGSPGTYKVHVKEPSEVEVLVEPR 1468
Query: 1442 MLKFTGIGEEQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAV 1478
LKF IGE + F+V+++ + +GYVFG L WSDG+H V+SP+AV
Sbjct: 1469 RLKFKRIGEVKMFKVILKAKVKGKPQGYVFGELIWSDGSHYVKSPLAV 1506
BLAST of MC00g0428 vs. ExPASy TrEMBL
Match:
A0A4Y1RKN9 (Subtilase family protein OS=Prunus dulcis OX=3755 GN=Prudu_015594 PE=3 SV=1)
HSP 1 Score: 1846 bits (4781), Expect = 0.0
Identity = 926/1485 (62.36%), Postives = 1136/1485 (76.50%), Query Frame = 0
Query: 5 VYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMIEEK 64
+YLG+ H PS+VDL T SHYNLLGS+LGSNE A+EAIFYSYNR+I+GFAA+++E+
Sbjct: 3 MYLGAQPHVLDPSSVDLNSVTNSHYNLLGSVLGSNERAQEAIFYSYNRNINGFAAILDEE 62
Query: 65 VAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIGNLD 124
A +AK+P V SV N+ RKLHTT SW+FLGLE +G + SIW A FG +TIIGNLD
Sbjct: 63 EAAQIAKDPNVLSVFPNRGRKLHTTRSWDFLGLEENGEVRPGSIWKKAQFGANTIIGNLD 122
Query: 125 TGVWPESKSFSDKGYGPIPSRWRGSCEGGSK--FHCNRKLIGARYFNKGYAAYAGPLNAS 184
TGVWPESKSFSD+G GPIPS+WRG C+ +K HCNRKLIGARYF+KGY AYA +N+S
Sbjct: 123 TGVWPESKSFSDEGIGPIPSKWRGICQLDTKNGSHCNRKLIGARYFSKGYLAYASTVNSS 182
Query: 185 -----YETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQV 244
ARD GHG+HTLSTA GNFV ASVFGNGNGTAKGGSP+ARV AYKVCWP +
Sbjct: 183 AAKTIQPNARDFGGHGSHTLSTAAGNFVPRASVFGNGNGTAKGGSPKARVAAYKVCWPPI 242
Query: 245 FSGGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSA 304
+G ECFDADI+AA +AAISDGVDVLSVSLGG++ + HAV+ G+ VV SA
Sbjct: 243 -NGNECFDADIMAAFDAAISDGVDVLSVSLGGEAAE---------VLHAVKKGISVVSSA 302
Query: 305 GNSGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLI 364
GNSGP PGTV N++PWL+TVGASTI+R F+SYVALGN+KH+KG SLS LP++ FYPLI
Sbjct: 303 GNSGPTPGTVSNVSPWLLTVGASTIDREFSSYVALGNKKHLKGASLSSGALPSKMFYPLI 362
Query: 365 SAVDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILA 424
SAVDAKA N S+ +AQ+C GSL+ KKV+GKI+VC+RGEN R DKG A AGAVGMIL
Sbjct: 363 SAVDAKAANASSSDAQLCKAGSLEKKKVEGKILVCIRGENARADKGQQAVLAGAVGMILV 422
Query: 425 NAQENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMAS 484
N + +G+E++AD HLLP SHV+Y+DG+ VF YIKSTK P+AY+T V+TE+G KPAP MAS
Sbjct: 423 NDKLSGNEIIADPHLLPTSHVNYSDGKAVFAYIKSTKTPVAYLTRVKTEVGAKPAPFMAS 482
Query: 485 FSSRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHI 544
FSSRGPNTIE+SILKPDITAPGV+IIAA++ P+ FD RR+ +N SGTSMSCPH+
Sbjct: 483 FSSRGPNTIEQSILKPDITAPGVSIIAAYTGAEGPTDQKFDKRRVSFNTESGTSMSCPHV 542
Query: 545 SGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRA 604
SGIVGLLKTL+P WSPAAIKSAIMTTA R N + ++ K +A P AYGAGHV+P+RA
Sbjct: 543 SGIVGLLKTLHPSWSPAAIKSAIMTTARKRDNKKEAMQDSSKARATPFAYGAGHVQPNRA 602
Query: 605 VNPGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQSFKLTDFNYPSISIPNLKSG 664
++PGLVYDL+T+DYLNFLC+RGYN T ++ FSN C +++ L DFNYPSI++P+L
Sbjct: 603 MDPGLVYDLTTDDYLNFLCSRGYNATLLKVFSNEPHTCPKAYSLADFNYPSITVPDLHDK 662
Query: 665 PVTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRR 724
PVT+ RRVKNVGS GTYV +K P GVSVSV+P L+F I EEK FKVV++ +G +
Sbjct: 663 PVTVTRRVKNVGSPGTYVVHIKEPAGVSVSVKPGSLQFKTIGEEKKFKVVLKPKVQGTQ- 722
Query: 725 GYVFGSLAWSDGKRHVRSPIAVNLSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLG 784
YVFG L WSDGK +VRSPI S+I YLG+HSHG NPS++DL + HY+ LGS L
Sbjct: 723 DYVFGELNWSDGKHNVRSPI----SFIVYLGAHSHGPNPSSVDLDSVRKFHYDFLGSFLR 782
Query: 785 SNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGL 844
SN +AKD IFYSY ++INGFAAIL+E+ A ++A+HP+V+SV NK KL TT SW+FLGL
Sbjct: 783 SNKSAKDVIFYSYTRHINGFAAILEEEEAADIAEHPNVISVFLNKGSKLETTRSWNFLGL 842
Query: 845 ENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSK-F 904
E +G IPS SIW A GE TII N+DTGVWPESKSFSD+G GP+PS+WRG C+ +K
Sbjct: 843 ERNGLIPSYSIWMKARLGEDTIIANIDTGVWPESKSFSDEGLGPVPSKWRGICQHDTKRV 902
Query: 905 RCNRKLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNG 964
RCNRKLIG RYFN G GPLN+S+ TARD +GHG+HTL+TA GNFV G SVFGNGNG
Sbjct: 903 RCNRKLIGTRYFNNGLAMYAGPLNSSFSTARDYDGHGSHTLATAAGNFVPGVSVFGNGNG 962
Query: 965 TAKGGSPRARVAAYRVCWPAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDD 1024
TAKGGSPRARVAAY+VCW CF AD+LA F+AAI DGVD++SVSLGG +EF
Sbjct: 963 TAKGGSPRARVAAYKVCWAPYEGVQCFDADVLAAFDAAISDGVDIISVSLGGGAQEFFKS 1022
Query: 1025 GLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKH 1084
++IGAFHAV+HGI VV +AGN+GP GTV N++PW++TVGA T DR F SYV LGN+KH
Sbjct: 1023 SISIGAFHAVKHGIVVVSAAGNTGPNPGTVLNLSPWLLTVGAGTIDREFTSYVSLGNKKH 1082
Query: 1085 IKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLR--G 1144
+KG SLS K LP++KFYPL+S A+AK +N S A +C+ G+LDP+KV+GKI+VCLR
Sbjct: 1083 LKGVSLSAKGLPSEKFYPLVSAAEAKHANASTAEAIICQGGTLDPRKVKGKILVCLREYN 1142
Query: 1145 DNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKI 1204
DNAR +K + A GAVGMIL N + +G++++AD H+L SHV+Y DG+ IF YIKSTK
Sbjct: 1143 DNARTEKSWQADMEGAVGMILVNDEQSGNDVVADPHVLLVSHVNYTDGKYIFDYIKSTKT 1202
Query: 1205 PMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGS 1264
PMAY+T VKTELG KPAPF PDIIAPGVSIIAAY+E A P+
Sbjct: 1203 PMAYLTRVKTELGSKPAPF------------------PDIIAPGVSIIAAYTEAAGPTSQ 1262
Query: 1265 SFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPIL 1324
D RR PFN ++G+SM+CPH SGI GLL+T +P WSPAAIKSA+MTTA T+ + + PIL
Sbjct: 1263 ISDTRRVPFNVQTGSSMACPHASGIAGLLRTLHPDWSPAAIKSAIMTTATTQDDSMEPIL 1322
Query: 1325 N-TTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFA 1384
+ ++ +KA P +YG+GH++PNKA++PGLVY+LT DYLNFLCA GYN+T IK FS++ +
Sbjct: 1323 DDSSYVKATPFAYGSGHIQPNKAMDPGLVYNLTTLDYLNFLCAHGYNETMIKSFSNSTYK 1382
Query: 1385 CSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLK 1444
CS SF L DFNYPSIS+PNL PV R+V NVGSPGTY VK P V V V+P LK
Sbjct: 1383 CSKSFSLADFNYPSISVPNLCEDPVTINRKVTNVGSPGTYKVHVKEPSEVEVLVQPRRLK 1442
Query: 1445 FTGIGEEQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAV 1478
F IGE + F+V+++ + +GYVFG L WSDG+H V+SP+AV
Sbjct: 1443 FKRIGEVKMFKVILKAKVKGKPQGYVFGELIWSDGSHYVKSPLAV 1454
BLAST of MC00g0428 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 890.6 bits (2300), Expect = 1.8e-258
Identity = 439/743 (59.08%), Postives = 545/743 (73.35%), Query Frame = 0
Query: 2 SYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMI 61
SY+VY G+HSH + ++ E+HY+ LGS GS E A +AIFYSY +HI+GFAA +
Sbjct: 31 SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90
Query: 62 EEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIG 121
+ +A +++K+P V SV NKA KLHTT SW+FLGLEH+ +PS+SIW A FGE TII
Sbjct: 91 DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150
Query: 122 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEG--GSKFHCNRKLIGARYFNKGYAAYAGPL 181
NLDTGVWPESKSF D+G GPIPSRW+G C+ + FHCNRKLIGARYFNKGYAA G L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210
Query: 182 NASYETARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQVFS 241
N+S+++ RD DGHG+HTLSTA G+FV G S+FG GNGTAKGGSPRARV AYKVCWP V
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPV-K 270
Query: 242 GGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCSAGN 301
G EC+DAD+LAA +AAI DG DV+SVSLGG+ F +D AIG+FHA + ++VVCSAGN
Sbjct: 271 GNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGN 330
Query: 302 SGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPLISA 361
SGPA TV N+APW +TVGAST++R F S + LGN KH KG SLS LP KFYP++++
Sbjct: 331 SGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMAS 390
Query: 362 VDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMILANA 421
V+AKAKN SA +AQ+C GSLDP K KGKI+VCLRG+NGRV+KG A G +GM+L N
Sbjct: 391 VNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENT 450
Query: 422 QENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMASFS 481
G++LLAD H+LPA+ ++ D V +YI TK P+A++T RT+LG+KPAP+MASFS
Sbjct: 451 YVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFS 510
Query: 482 SRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPHISG 541
S+GP+ + ILKPDITAPGV++IAA++ P+ FD RR+ +N +SGTSMSCPHISG
Sbjct: 511 SKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISG 570
Query: 542 IVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHKANPLAYGAGHVRPSRAVN 601
I GLLKT YP WSPAAI+SAIMTTA + PI N KA P ++GAGHV+P+ AVN
Sbjct: 571 IAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVN 630
Query: 602 PGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVCSQ-SFKLTDFNYPSISIPNLKSGP 661
PGLVYDL DYLNFLC+ GYN +QI FS +F CS L + NYPSI++PNL S
Sbjct: 631 PGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSK 690
Query: 662 VTIRRRVKNVGSAGTYVARVKVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVGKGKRRG 721
VT+ R VKNVG Y +V P GV V+V+P L FT++ E+K FKV++ + +G
Sbjct: 691 VTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKG 750
Query: 722 YVFGSLAWSDGKRHVRSPIAVNL 742
YVFG L WSD K VRSPI V L
Sbjct: 751 YVFGELVWSDKKHRVRSPIVVKL 772
BLAST of MC00g0428 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 884.4 bits (2284), Expect = 1.3e-256
Identity = 451/745 (60.54%), Postives = 548/745 (73.56%), Query Frame = 0
Query: 738 AVNLSYIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGF 797
A+ SYI YLGSH+H S+ L SH L S +GS+ AK+AIFYSY ++INGF
Sbjct: 37 ALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGF 96
Query: 798 AAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDGEIPSNSIWNLASFGES 857
AAILDE A E+AKHP VVSV NK RKLHTT SW+F+ L +G + +S+WN A +GE
Sbjct: 97 AAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGED 156
Query: 858 TIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCNRKLIGARYFNKGYVASIG 917
TII NLDTGVWPESKSFSD+GYG +P+RW+G C CNRKLIGARYFNKGY+A G
Sbjct: 157 TIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCNRKLIGARYFNKGYLAYTG 216
Query: 918 -PLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPA 977
P NASYET RD +GHG+HTLSTA GNFV GA+VFG GNGTA GGSP+ARVAAY+VCWP
Sbjct: 217 LPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPP 276
Query: 978 VLTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLAIGAFHAVQHGITVVCSA 1037
V CF ADILA EAAI DGVDVLS S+GG ++ DG+AIG+FHAV++G+TVVCSA
Sbjct: 277 VDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSA 336
Query: 1038 GNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKGASLSDKILPAQKFYPLI 1097
GNSGP GTVSNVAPW+ITVGAS+ DR F ++V L N + KG SLS K LP +K Y LI
Sbjct: 337 GNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLS-KPLPEEKMYSLI 396
Query: 1098 STADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILA 1157
S ADA +N ++ A LC++GSLDPKKV+GKI+VCLRGDNARVDKG AA AGA GM+L
Sbjct: 397 SAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLC 456
Query: 1158 NAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYMTHVKTELGVKPAPFMAS 1217
N + +G+E+++DAH+LPAS + Y DGE +F Y+ STK P Y+ L KPAPFMAS
Sbjct: 457 NDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMAS 516
Query: 1218 FSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHV 1277
FSSRGPNTI ILKPDI APGV+IIAA++E P+ D RR+PFN ESGTSMSCPH+
Sbjct: 517 FSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHI 576
Query: 1278 SGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQLKANPLSYGAGHVRPNKA 1337
SG+VGLLKT +P WSPAAI+SA+MTT+ T+ N P+++ + KANP SYG+GHV+PNKA
Sbjct: 577 SGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKA 636
Query: 1338 LNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAP-FACSHSFKLTDFNYPSISIPNLKF 1397
+PGLVYDLT DYL+FLCA GYN T ++ F+ P + C L DFNYPSI++PNL
Sbjct: 637 AHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLT- 696
Query: 1398 GPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGEEQSFRVVVRRVENEER 1457
G + R++KNVG P TY A+ + P GV VSVEP L F GE + F++ +R +
Sbjct: 697 GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLP-VTP 756
Query: 1458 RGYVFGWLAWSDGNHRVRSPIAVNL 1481
GYVFG L W+D +H VRSPI V L
Sbjct: 757 SGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of MC00g0428 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 627.5 bits (1617), Expect = 2.8e-179
Identity = 346/748 (46.26%), Postives = 475/748 (63.50%), Query Frame = 0
Query: 2 SYIVYLGSHSHGSSPSAVDLELATESHYNLLGSLLGSNEAAKEAIFYSYNRHIDGFAAMI 61
+YIV++ + PS+ DL H N S L S + E + Y+Y I GF+ +
Sbjct: 31 TYIVHM---AKSQMPSSFDL------HSNWYDSSLRSISDSAE-LLYTYENAIHGFSTRL 90
Query: 62 EEKVAEDLAKNPAVASVHENKARKLHTTSSWNFLGLEHDGGIPSNSIWNLASFGESTIIG 121
++ A+ L P V SV +LHTT + FLGL+ + ++ A ++G
Sbjct: 91 TQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----TADLFPEAGSYSDVVVG 150
Query: 122 NLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFH---CNRKLIGARYFNKGYAAYAGP 181
LDTGVWPESKS+SD+G+GPIPS W+G CE G+ F CNRKLIGAR+F +GY + GP
Sbjct: 151 VLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGP 210
Query: 182 LNASYE--TARDHDGHGTHTLSTAGGNFVSGASVFGNGNGTAKGGSPRARVVAYKVCWPQ 241
++ S E + RD DGHGTHT STA G+ V GAS+ G +GTA+G +PRARV YKVCW
Sbjct: 211 IDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWL- 270
Query: 242 VFSGGECFDADILAAIEAAISDGVDVLSVSLGGDSKDFSDDVTAIGAFHAVQNGVIVVCS 301
G CF +DILAAI+ AI+D V+VLS+SLGG D+ D AIGAF A++ G++V CS
Sbjct: 271 ----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 330
Query: 302 AGNSGPAPGTVQNLAPWLVTVGASTINRLFTSYVALGNRKHIKGTSLSDKILPAQKFYPL 361
AGN+GP+ ++ N+APW+ TVGA T++R F + LGN K+ G SL K P
Sbjct: 331 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 390
Query: 362 ISAVDAKAKNVSAYEAQICDEGSLDPKKVKGKIIVCLRGENGRVDKGHVAAQAGAVGMIL 421
I A +A +A +C G+L P+KVKGKI++C RG N RV KG V AG VGMIL
Sbjct: 391 IYAGNAS----NATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMIL 450
Query: 422 ANAQENGDELLADAHLLPASHVSYADGELVFQYIKSTKNPMAYMTHVRTELGIKPAPLMA 481
AN NG+EL+ADAHLLPA+ V G+++ Y+ + NP A ++ + T +G+KP+P++A
Sbjct: 451 ANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVA 510
Query: 482 SFSSRGPNTIEESILKPDITAPGVNIIAAFSEDAPPSGSSFDNRRIPYNVVSGTSMSCPH 541
+FSSRGPN+I +ILKPD+ APGVNI+AA++ A P+G + D+RR+ +N++SGTSMSCPH
Sbjct: 511 AFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPH 570
Query: 542 ISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANNFHPIMNTIKHK-ANPLAYGAGHVRPS 601
+SG+ LLK+++P+WSPAAI+SA+MTTA + P+++ K + P +GAGHV P+
Sbjct: 571 VSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPT 630
Query: 602 RAVNPGLVYDLSTNDYLNFLCARGYNQTQIRKFSNTSFVC--SQSFKLTDFNYPSISIPN 661
A NPGL+YDL+T DYL FLCA Y QIR S ++ C S+S+ + D NYPS ++
Sbjct: 631 TATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNV 690
Query: 662 LKSGPVTIRRRVKNVGSAGTYVARV-KVPPGVSVSVEPNELKFTRIDEEKAFKVVVRRVG 721
G R V +VG AGTY +V GV +SVEP L F +E+K++ V
Sbjct: 691 DGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDS 750
Query: 722 KGKRRGYVFGSLAWSDGKRHVRSPIAVN 741
FGS+ WSDGK V SP+A++
Sbjct: 751 SKPSGSNSFGSIEWSDGKHVVGSPVAIS 755
BLAST of MC00g0428 vs. TAIR 10
Match:
AT5G45650.1 (subtilase family protein )
HSP 1 Score: 583.9 bits (1504), Expect = 3.5e-166
Identity = 345/777 (44.40%), Postives = 460/777 (59.20%), Query Frame = 0
Query: 743 YIFYLGSHSHGLNPSAIDLQLATESHYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAILD 802
YI Y G H I+ E H++ L S+ S A+ ++ YSY INGFAA L
Sbjct: 27 YIVYFGEHKGDKAFHEIE-----EHHHSYLQSVKESEEDARASLLYSYKHSINGFAAELT 86
Query: 803 EKVAQELAKHPSVVSVHENKARK--LHTTGSWSFLGL---ENDGEIP------------S 862
A +L K VVSV ++ RK HTT SW F+GL E D ++P
Sbjct: 87 PDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVG 146
Query: 863 NSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKF---RCNRKL 922
+ A G+ I+G LD+GVWPESKSF+DKG GP+P W+G C+ G F CNRK+
Sbjct: 147 RNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNRKI 206
Query: 923 IGARYFNKGYVASIGPLNAS----YETARDDEGHGTHTLSTAGGNFVSGASVFGN-GNGT 982
IGARY+ KGY G NA+ + + RD +GHG+HT STA G V GAS G G+
Sbjct: 207 IGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAKGS 266
Query: 983 AKGGSPRARVAAYRVCW-----PAVLTGGCFMADILAGFEAAIHDGVDVLSVSLGGS-PE 1042
A GG+P AR+A Y+ CW V C D+LA + AI DGV V+S+S+G + P
Sbjct: 267 ASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTEPF 326
Query: 1043 EFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGL 1102
F+ DG+A+GA HAV+ I V SAGNSGP GT+SN+APW+ITVGAST DR F + L
Sbjct: 327 PFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGLVL 386
Query: 1103 GNRKHIKGASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVC 1162
GN IK S++ KF PL+ ++ ++L C SL P+ V GK+V+C
Sbjct: 387 GNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVVLC 446
Query: 1163 LRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKS 1222
LRG +R+ KG +AG GMIL N NG+E+ +D+H +P + V+ + I +YIK+
Sbjct: 447 LRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYIKT 506
Query: 1223 TKIPMAYMTHVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASP 1282
K P A++ KT + AP M FSSRGPN ++ +ILKPDI APG+ I+AA+S SP
Sbjct: 507 DKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGADSP 566
Query: 1283 SGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLH 1342
S S D+R + +N SGTSMSCPHV+G + LLK +PKWS AAI+SALMTTA +
Sbjct: 567 SKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDKKK 626
Query: 1343 PILNTTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAP 1402
PI +TT L ANP + G+GH RP KA +PGLVYD + + YL + C+ N T I P
Sbjct: 627 PIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASYRAYLLYGCS--VNITNIDPTFKCP 686
Query: 1403 FACSHSFKLTDFNYPSISIPNLKFGPVKAKRRVKNVG---SPGTYVAQVKAPPGVAVSVE 1462
+ + NYPSI++PNLK V KR V NVG S TY+ VK P G++V
Sbjct: 687 SKIPPGY---NHNYPSIAVPNLK-KTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAI 746
Query: 1463 PNMLKFTGIGEEQSFRVVVRRVENE-----ERRGYVFGWLAWSDGNHRVRSPIAVNL 1481
PN+L F IG++Q F++V++ ++N+ E+ Y FGW +W+D H VRSPIAV+L
Sbjct: 747 PNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSL 790
BLAST of MC00g0428 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 569.7 bits (1467), Expect = 6.9e-162
Identity = 342/738 (46.34%), Postives = 454/738 (61.52%), Query Frame = 0
Query: 768 HYNLLGSLLGSNVAAKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLH 827
H +LL SL S A + YSY++ ++GF+A L L +HPSV+SV ++AR++H
Sbjct: 53 HVSLLRSLPSSPQPA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIH 112
Query: 828 TTGSWSFLGLENDGEIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWR 887
TT + +FLG ++ +W+ +++GE I+G LDTG+WPE SFSD G GPIPS W+
Sbjct: 113 TTHTPAFLGFSQ-----NSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWK 172
Query: 888 GSCEGGSKF---RCNRKLIGARYFNKGYVASIG--PLNASYE--TARDDEGHGTHTLSTA 947
G CE G F CNRKLIGAR F +GY+ +A+ E + RD EGHGTHT STA
Sbjct: 173 GECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTA 232
Query: 948 GGNFVSGASVFGNGNGTAKGGSPRARVAAYRVCWPAVLTGGCFMADILAGFEAAIHDGVD 1007
G+ V+ AS++ GTA G + +AR+AAY++CW TGGC+ +DILA + A+ DGV
Sbjct: 233 AGSVVANASLYQYARGTATGMASKARIAAYKICW----TGGCYDSDILAAMDQAVADGVH 292
Query: 1008 VLSVSLG--GSPEEFSDDGLAIGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGA 1067
V+S+S+G GS E+ D +AIGAF A +HGI V CSAGNSGP T +N+APW++TVGA
Sbjct: 293 VISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGA 352
Query: 1068 STADRLFASYVGLGNRKHIKGASL-SDKILPAQKFYPLISTADAKASNVSLEYAQLCEEG 1127
ST DR FA+ G+ K G SL + + LP + L+ + D ++LC G
Sbjct: 353 STVDREFAANAITGDGKVFTGTSLYAGESLPDSQL-SLVYSGDCG--------SRLCYPG 412
Query: 1128 SLDPKKVEGKIVVCLRGDNARVDKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHV 1187
L+ VEGKIV+C RG NARV+KG AG GMILAN ++G+EL AD+HL+PA+ V
Sbjct: 413 KLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMV 472
Query: 1188 SYADGELIFQYIKSTKIPMAYMTHVKTELGVK-PAPFMASFSSRGPNTIEESILKPDIIA 1247
G+ I YIK++ P A ++ + T +G P+P +A+FSSRGPN + ILKPD+IA
Sbjct: 473 GAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIA 532
Query: 1248 PGVSIIAAYSEEASPSGSSFDKRRSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIK 1307
PGV+I+A ++ P+ D RR FN SGTSMSCPHVSG+ LL+ +P WSPAAIK
Sbjct: 533 PGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWSPAAIK 592
Query: 1308 SALMTTAATKANDLHPILN-TTQLKANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLC 1367
SAL+TTA N PI + T +N +GAGHV PNKALNPGLVYD+ +K+Y+ FLC
Sbjct: 593 SALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLC 652
Query: 1368 ARGYNQTQIKKFSSAPF---ACSHSFKLT--DFNYPSISIPNLKFGP-VKAKRRVKNVGS 1427
A GY I F P AC S T D NYPS S+ G VK KR VKNVGS
Sbjct: 653 AVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKRVVKNVGS 712
Query: 1428 --PGTYVAQVKAPPGVAVSVEPNMLKFTG----IGEEQSFRVVVRRVENEERRGYVFGWL 1482
Y VK+P V + V P+ L F+ + E +F+ VV G+ FG +
Sbjct: 713 NVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSI 770
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9ZSP5 | 2.5e-257 | 59.08 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| F4JXC5 | 1.8e-255 | 60.54 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| I1N462 | 1.0e-218 | 55.06 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 3.9e-178 | 46.26 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| Q9FK76 | 4.9e-165 | 44.40 | Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_016900594.1 | 0.0 | 78.72 | PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo] | [more] |
| KAE8650095.1 | 0.0 | 76.82 | hypothetical protein Csa_011146 [Cucumis sativus] | [more] |
| KAG6577540.1 | 0.0 | 75.91 | Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_038904203.1 | 0.0 | 66.84 | uncharacterized protein LOC120090550 [Benincasa hispida] | [more] |
| XP_008449184.1 | 0.0 | 64.33 | PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S4DX85 | 0.0 | 78.72 | subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=... | [more] |
| A0A1S3BLG8 | 0.0 | 64.33 | subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491134 PE=3 SV=... | [more] |
| A0A6J1KUL8 | 0.0 | 62.98 | uncharacterized protein LOC111498820 OS=Cucurbita maxima OX=3661 GN=LOC111498820... | [more] |
| A0A6J5V7Q5 | 0.0 | 63.24 | Uncharacterized protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS35141 PE=3 S... | [more] |
| A0A4Y1RKN9 | 0.0 | 62.36 | Subtilase family protein OS=Prunus dulcis OX=3755 GN=Prudu_015594 PE=3 SV=1 | [more] |
