MC00g0153 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0153
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	DNA-directed RNA polymerase V subunit 5A
Location	scaffold15: 581189 .. 586386 (-)
RNA-Seq Expression	MC00g0153
Synteny	MC00g0153

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5polypeptideCDSutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATAACCAAAATAAAGCATCAAAATTTAATCAAGAAACGTTGAGCGAGCTCTATTCGCAGGGAGAGAGAGCGAAAATGGAGGAAGATGGGTTTGAGGGAGAACCGAATAGAGTGGAGCTAGGGACTTGCTTGAGTAGCTACATCGACAATGGCTCCACAGAGAGCCACAGATACTTCCTTTGCCGAAGAACAGTTCTGGAGATGCTTAGGGACCGCGGTTACACTCCCACCACCTCTGATGCCGACCTCACTCTCCAACAATTTCGAGAAATTTACGGCCAATCGCCCGATGTTGATCGCCTCAAACTCTCCGCCGTTCATCGCTCCGATCCCTCCAAAAGAGTAAGACTCCATGCCTTCCTGTGAACCTTTTTTTTTCATTTGATTCTGTAAATCCTCGGTTTGTTAATGCCATGGGGTGGGAGATCAACGCAATGATGTGTTATGGTTTCTCGGGAGAGTGGATTAGGCATGAAGACTCCGCCATGGTTTAGTTTTGGTTACTTCGGGATTGTTGATTCGGCATATTACTTTTAGGTTTCAGTCAGTAGATGGTGCTGCATGTTTTCTTTTTTGATTTTCATTAAAATGCAGTAGACCAGACTATAGTTCTTATGAATTGTGATCATGGAGTAAAGTTTCTCTACGACGGATTCTTATAATGATACTTTACAACTATGTAAGTAGTCTAACATCTTTATTTATTCTTTCAAACTGTTTTTGTGGCTTTCCAAAAATCTTCAACATCTAGCTCCTACTTGATTGTGTTTACTTACCTGGATTTAGAAGTATGCACTTCTTTTGGCTGGTTGGAAATATCTCATTGTATTTGTATGGTTTAACCAGTAGTAGATCTTTGAGATTCAAATATTTTATCTCCCAACCGTGCATTGGCTCTTCGGTTATGAGGTTGTTCTACCTAACAATATTTCTTTTTGACTGATAAGTAAATGTTTATGCTATGTGTTCATAGTTTTGGCTTGTTTTGCACTCCTTTAGGTTCACTTGAAAGTAGCAGTAGCACCTTTCCCTTCAAACCTGATACTTTGTTTTGCAGATCCTGGTAATATTTTGTGGGACTGGTTCAGTCAAGGTTAATGCTATTCGCAATATTGCTGGCCAAATTGTTAATAAAGACACTTTGCACGGTCTCATTCTAATTGTGCAAAATCAAATTACAAACCAAGCTATGAAAGCAGTTGATCTTTTCTCATTCAAGGTTGAAATATTCCAGGTTTGTTTCTTGAAACTGTTTATGCTTTTCTTTGGTTGACAGTGGCATCTTGAAAAATACTTGGTTTCAACAAGTCATTTATTTGTTGTCATGTTAAATTTTAGTATTTTCTTCTCACTTCAGTTTTTAGTGTTTTCTTCTTAGTGGACACTGCTGAGGCTGTGATCTACGTATAATAAAAACGTAGGCTGGTTAACTAAAAAAATTAAATGCATCATTAATCAGTAACGTACTATAATGGTTAAAGGAAAAAGAAGCCTTCGATTGCTATATCATTGGGACTATGAATGTAAAAATAATAAAATATATACTAGATATTTGGAGGTGGTATATTTGCTTACATGCACAGAACTGTAGAGAAGCTTGACCATTTTGTTAACGTGATTGTAATGGGGAACAAATAAGGGAGAAAACAACATTGGACGTGAAATATGTCAAAATTTTTTGCTTCCTCTTATACTTCAAACCTACAATTTGTTTAATGAAAACCGGATTTTGGAATCAATAATGTTGTTGCTATGGCAGTGGTAAGTGGTAACAATTGCAGTAGAAAGTTGCTTTAGAAGCCAAAATTTAAAGTTAAGGCTGCAGATAATCTAAAAAAGTTCATATGTTGAATTTTTATGTTACTCATTTTTCTCTAATACTTTTATGTCATGTCTTATCAAATTTTTAAGACATTATTCTAAATTTCCTCTATGATTTCTATTCACTGTTATTTTTTGTCTGACATCAGATTACTGACTTACTCGTTAATATCACAAAGCATGCGTTAAAGCCAAACCATCAAGTGCTAACCGAGGAAGAAAAACAAAAGCTTTTGAAACAATACAGCATAGAGGAGAAGCAGGTAAGATATCGATCCAATGAAGTATCATAAACTATATTTAGACAGCTTACTTTTCTATATATAATCTTTCACTGTTATTTGCTTTGGTGGACAACTTAATTATGAAGTATATTTCCTTCTGCACAATTGCTTGTAGATTAAATGCTGGATATTTTCCTCTCAAATGGAATTAATAAACCAAAGCCCAAAAGAGCAAAAACATCCTAATGGTGGAGGGAAGAGAAAACCCTCCCCAAAGAACTATAAAACCGTCGCTGCCTTATGCTGGATATTTTCCTCTCTGCCGTTGCCTTATGCTTTAAAAACCCTTAATAAAATATTCCAAGGAATGAAACTCTTTCTCATGTTAAACATGCTTTACTTGAATGACCAACAATTTTTCCTTTCTAATATTTATGTTAAATGCAGATATGAGTCATACATCAATGAACATCTTTCAATATTATTATAGCTGGAACTCTGAGCGAGCAAATGACATAGTATCTCATTCGAGACTATTAACTTTTTGGCAAAACTTATCCAAAGTATGACATGATATAGACTTATTTCGTTCCGCAAATCATTAGGTTCTTTTAGTGGCTATGCAGTGAATGATTGGAAAGTATTAATAATTGTGGTTTTAGACTTTGGGCTGAAGTGACTCAGCCTATCCCATAAATAGAAAAGTGCCTCAGCCCACAGTACATTGCTTCATTACCTCAGTTAGAATCTTATAATTGAATTTTTTGTGCTTGGTAGAAGGAATATTCAACTTCGAAGAGCTCTTTCCCATTCTTTTAGTTTCTAAGAAATAAAAGAACGGGAGGTTTGACTAAGCCCATGGTACGCCAACGTCACTTCAGTTGGAAGCTAATAATGGTAATTTTTGTGCTTGGTAAAAGAAATGTGTTGAACTTATTTTTTTTAGTGTAAGAAACAATTTCATTGATGTGTGAAATTACAAAAGGCTCTTAAGAAGATTTACATGAATCACTTCCAATTAACTAATAGGGAAGTGTAACTATCATGATGAAAAAAGGAGTGTTCTTACGCCAAGTGACAACTTTGTAAACAATAGCATCAAAAAACTTCTCATAAGGATGTGGTTTATCGTTGAAGGTTCTGTGGTTTATCGTTGAAATGTGTTGAACTTTGAAGAGCTATTTTCCTCGGGTTTTATGACTTGAGTATCTGAGTTACTACATTAGAGCACAAAAACACAACTTCATTTCCCTTGCTTGGATCAATTTGTCTAATGAGACTACACAGGGTGAGGGAGCTAAACATTTTTCAAGTTGTTGAAGGTTCCAAACAGGATTTTTTTTTCAATATTTTGTTTATGAGAAACAAATATTTCATTAATGTTATGAAATATTACAAAAGGGGAGGAAAACCACAAAAGACTGGGAGTTACAAAAAAACTCTCCAATTTAACAAAAGATCATCGTGGCTATAATTTGTAGAATGACTGTAAAAACATGTACACCAGCTAAGAGCCTTTACAAAACCTGATTCTGAAAAGGATTCAAAGGAGTGGTATTTGTTGTTTAAGAGTCTGTTGTTCCTTTCTATCCAAAGAGTTCATGGAAAAGTTGTAATGAGTTGGGTCCAAAGAATGCCCTTCTCCTCCTTGAAAGGATAGCCATCGAGAAAAGCATGGAGGATAGAGTTCAGATTCGTGTGGATAGGACTGACAGCAGAAGGAGAGAAGGATCTTGGTCCAGAATCTATGAGCAAAGACAGTTAAACAGGTGGCCTTAGGATTCAGCATTCTTCATGCACATGATGCAGCAGCTTGGGTAAATGGCCATATTAGGGAGCCTTCTTTGGAGAATGTCCTGAGTATTTATAGAAGCATGACTGAGTACCCAGCAAAAAAACTGTGAAAACCATAGTATGTGGTATACAAATGAATCATAGTAATACAACCCTCTGGTAGACAAATGAATCAAAGTAATACTACCCTTGATTTTGAGCATAACCATTGGGGTTGCCATTTTGCTTAGTCACTGTGGGCTTGCTTTTTGGAGATGCTCAATGTTGGTTCGGTGTACAATAGAGACTGTCGTTTTATGATAGAGGAGGTGTTATTGCATTCTCCCGTTCAGGATAGGGGCAGAGTTCTTTGGCATGCTTGTTTTTCCCATCTTGTGGGGTACTTGGCTTAATAGAGACTGCCGTTTTATGATAGAGGAGGTGTTATTGCATTCTCCCATGCGAGATAGGGGCAGAGTTCTTTGGCATTCTTGTTTTTCCCGTCTTGTGGGGTACTTGGCTTAATAGAGACTGCCGTTTTATGATAGAGGAGGTGTTATTGCATTCCCCCGTTCAGGATAGGGGCAGAGTTCTTTGGCATGCTTGTTTTTCCCGTCTTGTCGGGTACTTGGCTTAAGAGGAATAGTAGAATTATCAGAGGGTTTGAGAGGTCATGGAAATAAGTTTGGGATATTGTTAGATTTAATACCTCTTTGTAGGCAACGGTCAAGCCTTTTGTAATTATTTTCTAGGTCTTATTCTTTTGGGTTGAAGTCCCTTTCTGATTAGTTAATGTTTGTTTGGGCCTTTGGGCTTCTTTTTATGCTCTCCTGCATTGTTTCATTTTATTTAAAAAAGTATAACCCTTAATGCCATGTGTAATTACTGTTTAAATTCTCATGCACGATAAATTTCTTAAACTACCAACAGTTGCTTAAAGTTTTTATGCGTACTTTTGATACAATGCAGCTTCCTCGCATGCTACGCACTGATGCTATTGCTCGATACTATGGACTTGAGAAGGGGCAAGTCGTAAAAGTGACTTACGATGGCGATATGACCATGTCGCATGTAACTTACCGTTGTGTCTGGTGATAGCGTATAAATGCAGGTTTCGCCAACCATATTTTGGATTTTTTTCTCATTGAACTTGATTAGTTTTGTCTCTTAAACATGTATGCTCTAGAACTCCTTGTATTTACTAGGTTTCCAGTGTAGATTTTCATGACTACAGGTTTCTGCAAGATATTTGTATCCACAATTACTGCAGACATGACTGAAAGGAGGTACTTGAAACTCCCTCCCGCCCCAACCCCGCCCCAGATATTTTGAAATATGCAAGTTGTGTATTAAAGGATAACCACATGAATCAGATAAGG

mRNA sequence

GGATAACCAAAATAAAGCATCAAAATTTAATCAAGAAACGTTGAGCGAGCTCTATTCGCAGGGAGAGAGAGCGAAAATGGAGGAAGATGGGTTTGAGGGAGAACCGAATAGAGTGGAGCTAGGGACTTGCTTGAGTAGCTACATCGACAATGGCTCCACAGAGAGCCACAGATACTTCCTTTGCCGAAGAACAGTTCTGGAGATGCTTAGGGACCGCGGTTACACTCCCACCACCTCTGATGCCGACCTCACTCTCCAACAATTTCGAGAAATTTACGGCCAATCGCCCGATGTTGATCGCCTCAAACTCTCCGCCGTTCATCGCTCCGATCCCTCCAAAAGAATCCTGGTAATATTTTGTGGGACTGGTTCAGTCAAGGTTAATGCTATTCGCAATATTGCTGGCCAAATTGTTAATAAAGACACTTTGCACGGTCTCATTCTAATTGTGCAAAATCAAATTACAAACCAAGCTATGAAAGCAGTTGATCTTTTCTCATTCAAGGTTGAAATATTCCAGATTACTGACTTACTCGTTAATATCACAAAGCATGCGTTAAAGCCAAACCATCAAGTGCTAACCGAGGAAGAAAAACAAAAGCTTTTGAAACAATACAGCATAGAGGAGAAGCAGCTTCCTCGCATGCTACGCACTGATGCTATTGCTCGATACTATGGACTTGAGAAGGGGCAAGTCGTAAAAGTGACTTACGATGGCGATATGACCATGTCGCATGTAACTTACCGTTGTGTCTGGTGATAGCGTATAAATGCAGGTTTCGCCAACCATATTTTGGATTTTTTTCTCATTGAACTTGATTAGTTTTGTCTCTTAAACATGTATGCTCTAGAACTCCTTGTATTTACTAGGTTTCCAGTGTAGATTTTCATGACTACAGGTTTCTGCAAGATATTTGTATCCACAATTACTGCAGACATGACTGAAAGGAGGTACTTGAAACTCCCTCCCGCCCCAACCCCGCCCCAGATATTTTGAAATATGCAAGTTGTGTATTAAAGGATAACCACATGAATCAGATAAGG

Coding sequence (CDS)

ATGGAGGAAGATGGGTTTGAGGGAGAACCGAATAGAGTGGAGCTAGGGACTTGCTTGAGTAGCTACATCGACAATGGCTCCACAGAGAGCCACAGATACTTCCTTTGCCGAAGAACAGTTCTGGAGATGCTTAGGGACCGCGGTTACACTCCCACCACCTCTGATGCCGACCTCACTCTCCAACAATTTCGAGAAATTTACGGCCAATCGCCCGATGTTGATCGCCTCAAACTCTCCGCCGTTCATCGCTCCGATCCCTCCAAAAGAATCCTGGTAATATTTTGTGGGACTGGTTCAGTCAAGGTTAATGCTATTCGCAATATTGCTGGCCAAATTGTTAATAAAGACACTTTGCACGGTCTCATTCTAATTGTGCAAAATCAAATTACAAACCAAGCTATGAAAGCAGTTGATCTTTTCTCATTCAAGGTTGAAATATTCCAGATTACTGACTTACTCGTTAATATCACAAAGCATGCGTTAAAGCCAAACCATCAAGTGCTAACCGAGGAAGAAAAACAAAAGCTTTTGAAACAATACAGCATAGAGGAGAAGCAGCTTCCTCGCATGCTACGCACTGATGCTATTGCTCGATACTATGGACTTGAGAAGGGGCAAGTCGTAAAAGTGACTTACGATGGCGATATGACCATGTCGCATGTAACTTACCGTTGTGTCTGGTGA

Protein sequence

MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW

Homology

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BLAST of MC00g0153 vs. ExPASy Swiss-Prot
Match: Q9M1J2 (DNA-directed RNA polymerase V subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPE5A PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-76
Identity = 135/210 (64.29%), Postives = 173/210 (82.38%), Query Frame = 0


Query: 18  CLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQSPDVDRLK 77
           CLS Y+D  S ESHRY+L RR  L+MLRDRGY  +  D +L+L  FR +YG+ PDVDRL+
Sbjct: 13  CLSKYVDLSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLR 72

Query: 78  LSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAV 137
           +SA+HRSD +K++ ++F GT  VKVNAIR++   I++++T+ GLIL++QN +TNQA+KA+
Sbjct: 73  ISALHRSDSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAI 132

Query: 138 DLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIA 197
           +LFSFKVEIFQITDLLVNITKH+LKP HQVL +EEK  LLK++SIEEKQLPR+ + DAI 
Sbjct: 133 ELFSFKVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIV 192

Query: 198 RYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 228
           RYYGLEKGQVVKV Y G++T SHV +RCVW
Sbjct: 193 RYYGLEKGQVVKVNYRGELTESHVAFRCVW 222

[Close]

BLAST of MC00g0153 vs. ExPASy Swiss-Prot
Match: Q9ZVB9 (DNA-directed RNA polymerases IV and V subunit 5B OS=Arabidopsis thaliana OX=3702 GN=NRPD5B PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 3.5e-70
Identity = 129/220 (58.64%), Postives = 172/220 (78.18%), Query Frame = 0


Query: 7   EGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREI 66
           EG+   + +G  +S      S E H+Y+L RRT +EMLRDRGY  +  D +L+LQQFR +
Sbjct: 2   EGKGKEIVVGHSIS----KSSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRAL 61

Query: 67  YGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQ 126
           YG+ PDVD L++SA HR D SK+I V+FCGTG VKVNA+R IA  +++++ + GLIL++Q
Sbjct: 62  YGEHPDVDLLRISAKHRFDSSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQ 121

Query: 127 NQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQ 186
           + ITNQA+KAV+LFSFKVE+F+ITDLLVN++KH L+P HQVL ++EK+ LLK++SIEEKQ
Sbjct: 122 SHITNQALKAVELFSFKVELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQ 181

Query: 187 LPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCV 227
           LPR+   D I RYYGLE GQV+KVTY  +++ SHVTYRCV
Sbjct: 182 LPRLSSKDPIVRYYGLETGQVMKVTYKDELSESHVTYRCV 217

[Close]

BLAST of MC00g0153 vs. ExPASy Swiss-Prot
Match: Q9M1H8 (DNA-directed RNA polymerase V subunit 5C OS=Arabidopsis thaliana OX=3702 GN=NRPE5C PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 2.4e-50
Identity = 101/226 (44.69%), Postives = 149/226 (65.93%), Query Frame = 0


Query: 2   EEDGFEGEPNRVELGT-CLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 61
           EE   E   +  + GT C+S   D G  ES R++L R T  EMLRDRGY    ++  LTL
Sbjct: 7   EEGCCENVESTFDDGTNCISKTEDTGGIESKRFYLARTTAFEMLRDRGYEVNEAELSLTL 66

Query: 62  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 121
            +FR ++G+ P+++RL++    RSDP K+ILV+F GT  + V ++R +  QI N   LH 
Sbjct: 67  SEFRSVFGEKPELERLRICVPLRSDPKKKILVVFMGTEPITVKSVRALHIQISNNVGLHA 126

Query: 122 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 181
           +IL++Q+++ + A KA+  F F VE F I DLLVNITKH  +P  ++L +EEK++LL+++
Sbjct: 127 MILVLQSKMNHFAQKALTTFPFTVETFPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKH 186

Query: 182 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCV 227
           ++E+KQLP +   D+  RYYGL+K QVVK+TY  +     VTYRC+
Sbjct: 187 ALEDKQLPYLQEKDSFVRYYGLKKKQVVKITYSKEPVGDFVTYRCI 232

[Close]

BLAST of MC00g0153 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.3e-29
Identity = 75/200 (37.50%), Postives = 118/200 (59.00%), Query Frame = 0


Query: 29  ESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQSPDVDRLKLSAVHRSDPSK 88
           E  R +  ++T+++MLRDRGY    S+  +T QQF   +G +   + L      R+D S 
Sbjct: 6   ELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSD 65

Query: 89  RILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAVDLFS--FKVEI 148
           ++ + F     V V  ++    ++ +++     IL+VQ  +T  A   +   S  F +E+
Sbjct: 66  QLYIFFPDEAKVGVKTMKMYTNRMKSENVFRA-ILVVQQNLTPFARTCISEISSKFHLEV 125

Query: 149 FQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIARYYGLEKGQ 208
           FQ  ++LVNI +H L P HQVLT EEK+ LL++Y+++E QLPR+  TD IARY+GL++GQ
Sbjct: 126 FQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKRGQ 185

Query: 209 VVKVTYDGDMTMSHVTYRCV 227
           VVK+    +    +VTYR V
Sbjct: 186 VVKIIRPSETAGRYVTYRYV 204

[Close]

BLAST of MC00g0153 vs. ExPASy Swiss-Prot
Match: A8XGH1 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis briggsae OX=6238 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 4.3e-28
Identity = 69/205 (33.66%), Postives = 117/205 (57.07%), Query Frame = 0


Query: 29  ESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQSPDVDR-----LKLSAVHR 88
           E++R +  R+TVL+M+ DRGY     + D  L+ F+E +G  P   +     L +   H 
Sbjct: 7   ETYRLWRIRKTVLQMVHDRGYLVAQEELDQPLETFKETFGDRPSEKKPARSDLTILVAHN 66

Query: 89  SDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAVDLFS-- 148
            DP+ ++ V F     + +  I+ I  Q + +  +   I++VQ  +T  A +++   +  
Sbjct: 67  DDPADQMFVFFPEDTKIGIKTIKAICQQ-MQEQNISRAIIVVQTGMTPSAKQSIGDMAPK 126

Query: 149 FKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIARYYG 208
           + +E F   +L+VNIT+H L P H V+T EEK +LL +Y +++ QLPR+ + D +ARY+G
Sbjct: 127 YMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPVARYFG 186

Query: 209 LEKGQVVKVTYDGDMTMSHVTYRCV 227
           L +GQVVK+    +    ++TYR V
Sbjct: 187 LRRGQVVKIIRPSETAGRYITYRLV 210

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BLAST of MC00g0153 vs. NCBI nr
Match: XP_022158561.1 (DNA-directed RNA polymerase V subunit 5A [Momordica charantia])

HSP 1 Score: 451 bits (1160), Expect = 7.66e-160
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL
Sbjct: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG
Sbjct: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY
Sbjct: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. NCBI nr
Match: XP_038903402.1 (DNA-directed RNA polymerase V subunit 5A [Benincasa hispida] >XP_038903403.1 DNA-directed RNA polymerase V subunit 5A [Benincasa hispida])

HSP 1 Score: 417 bits (1073), Expect = 1.41e-146
Identity = 208/227 (91.63%), Postives = 218/227 (96.04%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGFEGEPN VE GTCLSSYIDNG+TESHRYFL RRTVLEMLRDRGYT  +SD DL+L
Sbjct: 1   MEEDGFEGEPNEVEFGTCLSSYIDNGTTESHRYFLSRRTVLEMLRDRGYTLNSSDIDLSL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQSPDVDRLKLSAVHRSDPSKR+LVIFCGTG+VKVNAIRNIAGQI NKD+LHG
Sbjct: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRVLVIFCGTGAVKVNAIRNIAGQIANKDSLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV+LFSFKVEIFQITDLLVNITKHALKPNHQVL+EEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVELFSFKVEIFQITDLLVNITKHALKPNHQVLSEEEKQKLLQQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLR DA+ARYYGLEKGQVVKVTYDGDMT SHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRMDAVARYYGLEKGQVVKVTYDGDMTRSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. NCBI nr
Match: XP_023539155.1 (DNA-directed RNA polymerase V subunit 5A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 410 bits (1055), Expect = 7.79e-144
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGF GEPNRVELGTCLSSYID+G+TESHRY+L RRTVLEMLRDRGYT   SD DLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQ PDVDRLKLSA H +DPSKRILVIFCGTG+VKV+AIRNIAGQIVNK+TLHG
Sbjct: 61  QQFREIYGQFPDVDRLKLSAAHGADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV++FSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQ+VKVTYDGDMT SHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. NCBI nr
Match: XP_022971518.1 (DNA-directed RNA polymerase V subunit 5A isoform X2 [Cucurbita maxima])

HSP 1 Score: 410 bits (1054), Expect = 1.11e-143
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGF GEPNRVELGTCLSSYID+G+TESHRY+L RRTVLEMLRDRGYT   SD DLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQ PDVDRLKLSA HR+DPSKRILVIFCGTG+VKV+AIRNIAGQIVNK+TLHG
Sbjct: 61  QQFREIYGQFPDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV++FSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYY LEKGQ+VKVTYDGDMT SHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYRLEKGQIVKVTYDGDMTRSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. NCBI nr
Match: XP_022950955.1 (DNA-directed RNA polymerase V subunit 5A isoform X2 [Cucurbita moschata])

HSP 1 Score: 409 bits (1052), Expect = 2.23e-143
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGF GEPNRVELGTCLSSYID+G+TESHRY+L RRTVLEMLRDRGYT   SD DLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQ  DVDRLKLSA HR+DPSKRILVIFCGTG+VKV+AIRNIAGQIVNK+TLHG
Sbjct: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV++FSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQ+VKVTYDGDMT SHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. ExPASy TrEMBL
Match: A0A6J1E191 (DNA-directed RNA polymerase V subunit 5A OS=Momordica charantia OX=3673 GN=LOC111025016 PE=3 SV=1)

HSP 1 Score: 451 bits (1160), Expect = 3.71e-160
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL
Sbjct: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG
Sbjct: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY
Sbjct: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. ExPASy TrEMBL
Match: A0A6J1I733 (DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111470215 PE=3 SV=1)

HSP 1 Score: 410 bits (1054), Expect = 5.36e-144
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGF GEPNRVELGTCLSSYID+G+TESHRY+L RRTVLEMLRDRGYT   SD DLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQ PDVDRLKLSA HR+DPSKRILVIFCGTG+VKV+AIRNIAGQIVNK+TLHG
Sbjct: 61  QQFREIYGQFPDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV++FSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYY LEKGQ+VKVTYDGDMT SHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYRLEKGQIVKVTYDGDMTRSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. ExPASy TrEMBL
Match: A0A6J1GH86 (DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453892 PE=3 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 1.08e-143
Identity = 206/227 (90.75%), Postives = 217/227 (95.59%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGF GEPNRVELGTCLSSYID+G+TESHRY+L RRTVLEMLRDRGYT   SD DLTL
Sbjct: 1   MEEDGFLGEPNRVELGTCLSSYIDDGTTESHRYYLSRRTVLEMLRDRGYTINPSDIDLTL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFREIYGQ  DVDRLKLSA HR+DPSKRILVIFCGTG+VKV+AIRNIAGQIVNK+TLHG
Sbjct: 61  QQFREIYGQFLDVDRLKLSAAHRADPSKRILVIFCGTGAVKVSAIRNIAGQIVNKETLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV++FSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLL+QY
Sbjct: 121 LILIVQNQITNQAMKAVEVFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLEQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQ+VKVTYDGDMT SHVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQIVKVTYDGDMTRSHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. ExPASy TrEMBL
Match: A0A1S3B7M5 (DNA-directed RNA polymerase V subunit 5A OS=Cucumis melo OX=3656 GN=LOC103486906 PE=3 SV=1)

HSP 1 Score: 402 bits (1032), Expect = 1.21e-140
Identity = 199/227 (87.67%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGFE EPN VE GTCLSSYID+G+TESHRYFL RRTV+EML+DRGYT  + D DL+L
Sbjct: 1   MEEDGFEAEPNPVEFGTCLSSYIDDGTTESHRYFLSRRTVVEMLKDRGYTINSPDIDLSL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFR+I+GQ PDVDRLKL A H SDPSKR+LV+FCGTG+VKVNAIRNIAGQI NKD+LHG
Sbjct: 61  QQFRDIFGQYPDVDRLKLYAAHHSDPSKRVLVVFCGTGAVKVNAIRNIAGQIANKDSLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV+LFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY
Sbjct: 121 LILIVQNQITNQAMKAVELFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMT +HVTYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTSAHVTYRCVW 227

[Close]

BLAST of MC00g0153 vs. ExPASy TrEMBL
Match: A0A0A0LCG9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822200 PE=3 SV=1)

HSP 1 Score: 399 bits (1026), Expect = 9.92e-140
Identity = 197/227 (86.78%), Postives = 212/227 (93.39%), Query Frame = 0


Query: 1   MEEDGFEGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 60
           MEEDGFE EPN VE GTCLSSYID+G+TESHRYFL RRTV+EML+DRGYT  + + D +L
Sbjct: 1   MEEDGFEAEPNPVEFGTCLSSYIDDGTTESHRYFLSRRTVVEMLKDRGYTINSPNIDFSL 60

Query: 61  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 120
           QQFRE +GQ PDVDRLKLSA H SDPSKR+LV+FCGTG+VKVNAIRNIAGQI NKD+LHG
Sbjct: 61  QQFRETFGQFPDVDRLKLSAAHHSDPSKRVLVVFCGTGAVKVNAIRNIAGQIANKDSLHG 120

Query: 121 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180
           LILIVQNQITNQAMKAV+LFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY
Sbjct: 121 LILIVQNQITNQAMKAVELFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 180

Query: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 227
           SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMT +H+TYRCVW
Sbjct: 181 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTRAHITYRCVW 227

[Close]

BLAST of MC00g0153 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 287.7 bits (735), Expect = 8.0e-78
Identity = 135/210 (64.29%), Postives = 173/210 (82.38%), Query Frame = 0


Query: 18  CLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQSPDVDRLK 77
           CLS Y+D  S ESHRY+L RR  L+MLRDRGY  +  D +L+L  FR +YG+ PDVDRL+
Sbjct: 13  CLSKYVDLSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLR 72

Query: 78  LSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAV 137
           +SA+HRSD +K++ ++F GT  VKVNAIR++   I++++T+ GLIL++QN +TNQA+KA+
Sbjct: 73  ISALHRSDSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAI 132

Query: 138 DLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIA 197
           +LFSFKVEIFQITDLLVNITKH+LKP HQVL +EEK  LLK++SIEEKQLPR+ + DAI 
Sbjct: 133 ELFSFKVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIV 192

Query: 198 RYYGLEKGQVVKVTYDGDMTMSHVTYRCVW 228
           RYYGLEKGQVVKV Y G++T SHV +RCVW
Sbjct: 193 RYYGLEKGQVVKVNYRGELTESHVAFRCVW 222

[Close]

BLAST of MC00g0153 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 266.2 bits (679), Expect = 2.5e-71
Identity = 129/220 (58.64%), Postives = 172/220 (78.18%), Query Frame = 0


Query: 7   EGEPNRVELGTCLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREI 66
           EG+   + +G  +S      S E H+Y+L RRT +EMLRDRGY  +  D +L+LQQFR +
Sbjct: 2   EGKGKEIVVGHSIS----KSSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRAL 61

Query: 67  YGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQ 126
           YG+ PDVD L++SA HR D SK+I V+FCGTG VKVNA+R IA  +++++ + GLIL++Q
Sbjct: 62  YGEHPDVDLLRISAKHRFDSSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQ 121

Query: 127 NQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQ 186
           + ITNQA+KAV+LFSFKVE+F+ITDLLVN++KH L+P HQVL ++EK+ LLK++SIEEKQ
Sbjct: 122 SHITNQALKAVELFSFKVELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQ 181

Query: 187 LPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCV 227
           LPR+   D I RYYGLE GQV+KVTY  +++ SHVTYRCV
Sbjct: 182 LPRLSSKDPIVRYYGLETGQVMKVTYKDELSESHVTYRCV 217

[Close]

BLAST of MC00g0153 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 200.3 bits (508), Expect = 1.7e-51
Identity = 101/226 (44.69%), Postives = 149/226 (65.93%), Query Frame = 0


Query: 2   EEDGFEGEPNRVELGT-CLSSYIDNGSTESHRYFLCRRTVLEMLRDRGYTPTTSDADLTL 61
           EE   E   +  + GT C+S   D G  ES R++L R T  EMLRDRGY    ++  LTL
Sbjct: 7   EEGCCENVESTFDDGTNCISKTEDTGGIESKRFYLARTTAFEMLRDRGYEVNEAELSLTL 66

Query: 62  QQFREIYGQSPDVDRLKLSAVHRSDPSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHG 121
            +FR ++G+ P+++RL++    RSDP K+ILV+F GT  + V ++R +  QI N   LH 
Sbjct: 67  SEFRSVFGEKPELERLRICVPLRSDPKKKILVVFMGTEPITVKSVRALHIQISNNVGLHA 126

Query: 122 LILIVQNQITNQAMKAVDLFSFKVEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQY 181
           +IL++Q+++ + A KA+  F F VE F I DLLVNITKH  +P  ++L +EEK++LL+++
Sbjct: 127 MILVLQSKMNHFAQKALTTFPFTVETFPIEDLLVNITKHIQQPKIEILNKEEKEQLLRKH 186

Query: 182 SIEEKQLPRMLRTDAIARYYGLEKGQVVKVTYDGDMTMSHVTYRCV 227
           ++E+KQLP +   D+  RYYGL+K QVVK+TY  +     VTYRC+
Sbjct: 187 ALEDKQLPYLQEKDSFVRYYGLKKKQVVKITYSKEPVGDFVTYRCI 232

[Close]

BLAST of MC00g0153 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-30
Identity = 75/200 (37.50%), Postives = 118/200 (59.00%), Query Frame = 0


Query: 29  ESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQSPDVDRLKLSAVHRSDPSK 88
           E  R +  ++T+++MLRDRGY    S+  +T QQF   +G +   + L      R+D S 
Sbjct: 6   ELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNSD 65

Query: 89  RILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAVDLFS--FKVEI 148
           ++ + F     V V  ++    ++ +++     IL+VQ  +T  A   +   S  F +E+
Sbjct: 66  QLYIFFPDEAKVGVKTMKMYTNRMKSENVFRA-ILVVQQNLTPFARTCISEISSKFHLEV 125

Query: 149 FQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIARYYGLEKGQ 208
           FQ  ++LVNI +H L P HQVLT EEK+ LL++Y+++E QLPR+  TD IARY+GL++GQ
Sbjct: 126 FQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGLKRGQ 185

Query: 209 VVKVTYDGDMTMSHVTYRCV 227
           VVK+    +    +VTYR V
Sbjct: 186 VVKIIRPSETAGRYVTYRYV 204

[Close]

BLAST of MC00g0153 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 119.0 bits (297), Expect = 4.9e-27
Identity = 74/201 (36.82%), Postives = 117/201 (58.21%), Query Frame = 0


Query: 29  ESHRYFLCRRTVLEMLRDRGYTPTTSDADLTLQQFREIYGQS---PDVDRLKLSAVHRSD 88
           E  R F  RRTVL+MLRDRGYT   SD +L  ++F + + ++    + + L +SA    +
Sbjct: 7   EITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKGPN 66

Query: 89  PSKRILVIFCGTGSVKVNAIRNIAGQIVNKDTLHGLILIVQNQITNQAMKAVDLFS--FK 148
           P+ +I V +     V V  I+      +  D +H  I++V   IT  A  AV   +    
Sbjct: 67  PADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKMLT 126

Query: 149 VEIFQITDLLVNITKHALKPNHQVLTEEEKQKLLKQYSIEEKQLPRMLRTDAIARYYGLE 208
           +E+F+  +L+ NIT+H L   + VL ++ K+KLL  Y++++ QLPR+L TD +ARYYGL+
Sbjct: 127 IEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYYGLK 186

Query: 209 KGQVVKVTYDGDMTMSHVTYR 225
           +GQVVK+      ++ + TYR
Sbjct: 187 RGQVVKIRRSDATSLDYYTYR 207

The following BLAST results are available for this feature:

BLAST of MC00g0153 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1J2	1.1e-76	64.29	DNA-directed RNA polymerase V subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPE...	[more]
Q9ZVB9	3.5e-70	58.64	DNA-directed RNA polymerases IV and V subunit 5B OS=Arabidopsis thaliana OX=3702...	[more]
Q9M1H8	2.4e-50	44.69	DNA-directed RNA polymerase V subunit 5C OS=Arabidopsis thaliana OX=3702 GN=NRPE...	[more]
O81098	2.3e-29	37.50	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
A8XGH1	4.3e-28	33.66	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis bri...	[more]

BLAST of MC00g0153 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158561.1	7.66e-160	100.00	DNA-directed RNA polymerase V subunit 5A [Momordica charantia]	[more]
XP_038903402.1	1.41e-146	91.63	DNA-directed RNA polymerase V subunit 5A [Benincasa hispida] >XP_038903403.1 DNA...	[more]
XP_023539155.1	7.79e-144	90.75	DNA-directed RNA polymerase V subunit 5A [Cucurbita pepo subsp. pepo]	[more]
XP_022971518.1	1.11e-143	90.75	DNA-directed RNA polymerase V subunit 5A isoform X2 [Cucurbita maxima]	[more]
XP_022950955.1	2.23e-143	90.75	DNA-directed RNA polymerase V subunit 5A isoform X2 [Cucurbita moschata]	[more]

BLAST of MC00g0153 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E191	3.71e-160	100.00	DNA-directed RNA polymerase V subunit 5A OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1I733	5.36e-144	90.75	DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1GH86	1.08e-143	90.75	DNA-directed RNA polymerase V subunit 5A isoform X2 OS=Cucurbita moschata OX=366...	[more]
A0A1S3B7M5	1.21e-140	87.67	DNA-directed RNA polymerase V subunit 5A OS=Cucumis melo OX=3656 GN=LOC103486906...	[more]
A0A0A0LCG9	9.92e-140	86.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G822200 PE=3 SV=1	[more]

BLAST of MC00g0153 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57080.1	8.0e-78	64.29	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT2G41340.1	2.5e-71	58.64	RNA polymerase II fifth largest subunit, D	[more]
AT3G54490.1	1.7e-51	44.69	RNA polymerase II fifth largest subunit, E	[more]
AT3G22320.1	1.6e-30	37.50	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	4.9e-27	36.82	RNA polymerase II fifth largest subunit, C	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 22..227 e-value: 6.0E-80 score: 265.5
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 24..151 e-value: 7.7E-36 score: 125.1
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 28..155
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 28..111 e-value: 3.3E-22 score: 78.9
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 154..226 e-value: 5.4E-30 score: 103.1
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 152..227 e-value: 5.1E-27 score: 95.3
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 152..227
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 9..227
None	No IPR available	PANTHER	PTHR10535:SF2	DNA-DIRECTED RNA POLYMERASE V SUBUNIT 5A	coord: 9..227

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0153.1	MC00g0153.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0055029	nuclear DNA-directed RNA polymerase complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity