MC00g0042 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0042
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	(1->3)-beta-glucan endohydrolase
Location	scaffold31: 778283 .. 779852 (-)
RNA-Seq Expression	MC00g0042
Synteny	MC00g0042

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTATAAATATCTTAATATGTTATTTTGTTTGTGTTTGGCAGGTGGTTTGGGTGGCATTGGGGTCAACTATGGGCTGAATGGTGACAATCTACCACAACCAAATGATGTTATAAGTCTGTATGGGCGGTGTGGTATCGATATCGTGCGGATATTTGAGCCTAATCACGGCGTTTTGGATGCGATGCGTGGGAAACCAAATGCCCTGTGGCTGGGAACAAGAAATGAAGACATACCCAGCTTCGCCATCAACCAAACAGCAGCCAACGCTTGGGTTAACGCTAACGTGGTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTCGGTAACGAAGTCATCCCTGCCGACCCAGCCGCACCTTTTGTTGCTAATGCCATTAGAAATATAATGCAGGCGCTTGCCATTACCGGAGTCAAGAAGGCCATAAAGGTAATGTGTAAGCTAAGTTTTTTTTTTTCTTTTTTTGTTTAAATTTGGTGTCACCGACATTTGGGTTTAGTTTCACTCAAATTTCTATAATTTCAAAATTTTAATTTTGTTAAGATGGTTAGATAGTTATTATTATTATATTGAACATGTAACATGTTTATTATTTATTTAGCAATGAATTAATGTTTTCTATCGAAGTGAGCATAACTCAACGGTAATTGATATATATCTTCAATCGAGAGGTTGTGAGTTCGATCCTCCACCCACATGTTGTGCTAAAAAAAAATTAATGTTTTCTATTTATATATTAGTTAACACAATATTAATTGAGTGATAAATAAAAAAATAAGGAAAATAAATAGATAGGTATCGTAAAAGTGACCATTTATGGATAAATCTGTGAGATTTAACCTAAACATAAATATATTAGATTAAAACTTTAAAATTTTATAGATGAAAATAAAAATTTTGAAAAGATTAAATTGAAATTAAGCCAAAGCCACGTAAACCAAGTTTGCAATTTAATCCTAAAAATTAAAAATTATCCAAATTTCTTATTATATATATATATACACTTGCACAGGTAACGACGGTGGTCCCTATGAGCGCCATGGGAGTCTCGTACCCACCCTCAGCTGGAACATTCACAGCTGTCGCCTTGGGGCCTCTAAGCAACATCGCAAAGGTGGTCGGTTCTAGCGGCGCACCACTACTATTGAATGTGTATCCATATTTTGCATACGCATCAAACCCACAACAGATTTCTTTAAATTATGCACTCTTCACTTCGACGACGCCTATTGTGGATGGAAATTTGCACTATTACAATCTGTTTGATGCTATGGTGGATTCTTTCTATGCGGCGTTAGAGAAGATTGGCGCTACCAATGTAACACTGGAGATTTCGGAGACTGGATGGCCGACTAAAGGGAATGAGCCGTTCACAAGTGTTGAGAATGCAGTGACATATAACAAAAATTTTGTGAACCATGTGAGCAGTGGTGCAGGAACTCCGAGGAGGCCGAATGTGAAGTATAGTGGAATTTTATTTGAAATGTTCAATGAAGATCTTAAGGCGGCTGGAATTGAGCAGAATTTTGGGTTCTTTTATCCTAATATGCACCCGGTTTATCCA

mRNA sequence

TTTTATAAATATCTTAATATGTTATTTTGTTTGTGTTTGGCAGGTGGTTTGGGTGGCATTGGGGTCAACTATGGGCTGAATGGTGACAATCTACCACAACCAAATGATGTTATAAGTCTGTATGGGCGGTGTGGTATCGATATCGTGCGGATATTTGAGCCTAATCACGGCGTTTTGGATGCGATGCGTGGGAAACCAAATGCCCTGTGGCTGGGAACAAGAAATGAAGACATACCCAGCTTCGCCATCAACCAAACAGCAGCCAACGCTTGGGTTAACGCTAACGTGGTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTCGGTAACGAAGTCATCCCTGCCGACCCAGCCGCACCTTTTGTTGCTAATGCCATTAGAAATATAATGCAGGCGCTTGCCATTACCGGAGTCAAGAAGGCCATAAAGGTAACGACGGTGGTCCCTATGAGCGCCATGGGAGTCTCGTACCCACCCTCAGCTGGAACATTCACAGCTGTCGCCTTGGGGCCTCTAAGCAACATCGCAAAGGTGGTCGGTTCTAGCGGCGCACCACTACTATTGAATGTGTATCCATATTTTGCATACGCATCAAACCCACAACAGATTTCTTTAAATTATGCACTCTTCACTTCGACGACGCCTATTGTGGATGGAAATTTGCACTATTACAATCTGTTTGATGCTATGGTGGATTCTTTCTATGCGGCGTTAGAGAAGATTGGCGCTACCAATGTAACACTGGAGATTTCGGAGACTGGATGGCCGACTAAAGGGAATGAGCCGTTCACAAGTGTTGAGAATGCAGTGACATATAACAAAAATTTTGTGAACCATGTGAGCAGTGGTGCAGGAACTCCGAGGAGGCCGAATGTGAAGTATAGTGGAATTTTATTTGAAATGTTCAATGAAGATCTTAAGGCGGCTGGAATTGAGCAGAATTTTGGGTTCTTTTATCCTAATATGCACCCGGTTTATCCA

Coding sequence (CDS)

TTTTATAAATATCTTAATATGTTATTTTGTTTGTGTTTGGCAGGTGGTTTGGGTGGCATTGGGGTCAACTATGGGCTGAATGGTGACAATCTACCACAACCAAATGATGTTATAAGTCTGTATGGGCGGTGTGGTATCGATATCGTGCGGATATTTGAGCCTAATCACGGCGTTTTGGATGCGATGCGTGGGAAACCAAATGCCCTGTGGCTGGGAACAAGAAATGAAGACATACCCAGCTTCGCCATCAACCAAACAGCAGCCAACGCTTGGGTTAACGCTAACGTGGTTCCTTATTATGCAGATGTCAATATCGCATACATCACCGTCGGTAACGAAGTCATCCCTGCCGACCCAGCCGCACCTTTTGTTGCTAATGCCATTAGAAATATAATGCAGGCGCTTGCCATTACCGGAGTCAAGAAGGCCATAAAGGTAACGACGGTGGTCCCTATGAGCGCCATGGGAGTCTCGTACCCACCCTCAGCTGGAACATTCACAGCTGTCGCCTTGGGGCCTCTAAGCAACATCGCAAAGGTGGTCGGTTCTAGCGGCGCACCACTACTATTGAATGTGTATCCATATTTTGCATACGCATCAAACCCACAACAGATTTCTTTAAATTATGCACTCTTCACTTCGACGACGCCTATTGTGGATGGAAATTTGCACTATTACAATCTGTTTGATGCTATGGTGGATTCTTTCTATGCGGCGTTAGAGAAGATTGGCGCTACCAATGTAACACTGGAGATTTCGGAGACTGGATGGCCGACTAAAGGGAATGAGCCGTTCACAAGTGTTGAGAATGCAGTGACATATAACAAAAATTTTGTGAACCATGTGAGCAGTGGTGCAGGAACTCCGAGGAGGCCGAATGTGAAGTATAGTGGAATTTTATTTGAAATGTTCAATGAAGATCTTAAGGCGGCTGGAATTGAGCAGAATTTTGGGTTCTTTTATCCTAATATGCACCCGGTTTATCCA

Protein sequence

FYKYLNMLFCLCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNFGFFYPNMHPVYP

Homology

[Close]

BLAST of MC00g0042 vs. ExPASy Swiss-Prot
Match: O22317 (Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 2.9e-81
Identity = 154/310 (49.68%), Postives = 203/310 (65.48%), Query Frame = 0


Query: 20  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNEDIP 79
           IGV YG+ G+NLP P++V+SLY    I  +R+++PN   L A+R     + L     D+ 
Sbjct: 29  IGVCYGMLGNNLPPPSEVVSLYKSNNIARMRLYDPNQAALQALRNSNIQVLLDVPRSDVQ 88

Query: 80  SFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAITG 139
           S A N +AA  W+  NVV Y+  V+  YI VGNE+IP    A ++  A+RNI  AL+  G
Sbjct: 89  SLASNPSAAGDWIRRNVVAYWPSVSFRYIAVGNELIPGSDLAQYILPAMRNIYNALSSAG 148

Query: 140 VKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFAYA 199
           ++  IKV+T V    +G SYPPSAG F++ A   LS I + + S+GAPLL+NVYPYF+Y 
Sbjct: 149 LQNQIKVSTAVDTGVLGTSYPPSAGAFSSAAQAYLSPIVQFLASNGAPLLVNVYPYFSYT 208

Query: 200 SNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETGWP 259
            NP QISL YALFT++  +V DG   Y NLFDA+VD+ +AALE++G  NV + +SE+GWP
Sbjct: 209 GNPGQISLPYALFTASGVVVQDGRFSYQNLFDAIVDAVFAALERVGGANVAVVVSESGWP 268

Query: 260 TKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNFG 319
           + G     S  NA TYN+N + HV  G GTPRRP  +    +FEMFNE+ KA GIEQNFG
Sbjct: 269 SAGGGAEASTSNARTYNQNLIRHV--GGGTPRRPGKEIEAYIFEMFNENQKAGGIEQNFG 328

Query: 320 FFYPNMHPVY 329
            FYPN  PVY
Sbjct: 329 LFYPNKQPVY 336

[Close]

BLAST of MC00g0042 vs. ExPASy Swiss-Prot
Match: O49352 (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN=BG4 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.5e-74
Identity = 148/312 (47.44%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 20  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRG-KPNALWLGTRNEDI 79
           IG+NYGL GDNLP P++VI+LY   GI  +RIF+PN  VL+A+RG +   + +G +++D+
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 80  PSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAIT 139
            + A ++ A   W  AN+  Y ADVNI +ITVGNEVIP  P  P V   ++++   +   
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG-PIGPQVLPVMQSLTNLVKSR 153

Query: 140 GVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFAY 199
            +   I ++TVV MS +  SYPPSAG FT+ A   L  + K++  +  P+L+N+YPYFAY
Sbjct: 154 NL--PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAY 213

Query: 200 ASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETGW 259
           AS+P  I L+YA F + + +V DG+L Y N+FDA+ D+F  A+EK G  N+ + +SETGW
Sbjct: 214 ASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGW 273

Query: 260 PTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNF 319
           P+ GN  FT+   A TYN+NFV H++SG GTP+RPN   +G LF  FNE+ K AG EQNF
Sbjct: 274 PSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNF 333

Query: 320 GFFYP-NMHPVY 329
           G + P +M P+Y
Sbjct: 334 GLYNPSDMKPIY 342

[Close]

BLAST of MC00g0042 vs. ExPASy Swiss-Prot
Match: O49353 (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN=BG5 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-71
Identity = 143/328 (43.60%), Postives = 209/328 (63.72%), Query Frame = 0


Query: 4   YLNMLFCLCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMR 63
           ++N    +        IG+NYGL GDNLP P+ VI+LY    I  +RIF+PN  VL+A+R
Sbjct: 27  FVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALR 86

Query: 64  G-KPNALWLGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAP 123
           G +  A+ +G R++D+ + + ++ A   W   N+ PY +D+NIA+ITVGNEVIP  P  P
Sbjct: 87  GHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPG-PIGP 146

Query: 124 FVANAIRNIMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVG 183
            V   ++++   +    +   I ++TVV M  +  SYPPSAG FT+ A   L  + K++ 
Sbjct: 147 QVLPVMQSLTNLVKSRNL--PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLS 206

Query: 184 SSGAPLLLNVYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALE 243
            + +P+L+ +YPYF+YAS+P  I L+YA F +   +V DG+L Y N+FDA+ D+F  A+E
Sbjct: 207 QTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAME 266

Query: 244 KIGATNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILF 303
           K G  ++ + +SETGWP+ GN   T+ + A TYN+NFV H++SG GTP+RPN    G LF
Sbjct: 267 KEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLF 326

Query: 304 EMFNEDLKAAGIEQNFGFFYPN-MHPVY 329
             FNE+ K  G EQNFG + PN M P+Y
Sbjct: 327 ATFNENQKPVGTEQNFGLYNPNDMKPIY 351

[Close]

BLAST of MC00g0042 vs. ExPASy Swiss-Prot
Match: Q02439 (Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4513 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.1e-68
Identity = 143/316 (45.25%), Postives = 196/316 (62.03%), Query Frame = 0


Query: 16  GLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRN 75
           G+ GIGVNYG+ G +LP P+ V++LY    I  VRIF P+  VL+A+R     + LGT N
Sbjct: 3   GVEGIGVNYGMMGSDLPSPDKVVALYKANNITDVRIFHPDTNVLEALRNSGLGVVLGTLN 62

Query: 76  EDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQAL 135
            D+   A + + A +WV++ V P+   V+  YI  GNEVIP + AA  V  A++N+  AL
Sbjct: 63  SDLAPLASDASYAASWVHSYVQPFAGAVSFRYINAGNEVIPGESAA-LVLPAMKNLEAAL 122

Query: 136 AITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPY 195
              G+  ++ VTT +  S +G SYPPS GTF+  AL  +  I   + SSG PLL+NVYPY
Sbjct: 123 QAAGL--SVPVTTAMATSVLGTSYPPSQGTFSEAALPTVGPIVSHLASSGTPLLVNVYPY 182

Query: 196 FAYASNPQQISLNYALFTST--TPIVDGNLHYYNLFDAMVDSFYAALEKI-GATNVTLEI 255
           FAY+++P  + L+YAL +S+    + D  + Y N+FDA++D+ YAA+EK  G  ++ L +
Sbjct: 183 FAYSADPSSVRLDYALLSSSAAVAVTDNGVEYANMFDAILDAVYAAVEKAGGGESLELVV 242

Query: 256 SETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAG 315
           SETGWP+ G     SVENA  Y  N V HV    GTPRRP       +F MFNE+ K  G
Sbjct: 243 SETGWPSGGGGYGASVENAAAYINNLVRHV---GGTPRRPGKAVETYIFAMFNENQKPEG 302

Query: 316 IEQNFGFFYPNMHPVY 329
           +EQNFG F P+M  VY
Sbjct: 303 VEQNFGMFQPDMSQVY 312

[Close]

BLAST of MC00g0042 vs. ExPASy Swiss-Prot
Match: P52408 (Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS1 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.4e-64
Identity = 136/310 (43.87%), Postives = 194/310 (62.58%), Query Frame = 0


Query: 20  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALWLGTRNEDIP 79
           IGV  G+ GD+LP   +V++LY    I  +R+++PN   L+A+RG    L LG  NE++ 
Sbjct: 40  IGVCNGMVGDDLPPQAEVVALYKTNNIPRMRLYDPNPAALEALRGSNIKLLLGVPNENLQ 99

Query: 80  SFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAITG 139
             A++Q  ANAWV  N V  YA+V   YI VGNEV P+D  A F+  A+RNI +A+++ G
Sbjct: 100 YIALSQANANAWVQNN-VRNYANVKFKYIAVGNEVKPSDSFAQFLVPAMRNIQEAISLAG 159

Query: 140 VKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFAYA 199
           + K IKV+T +    +G ++PPS G+F +     L  I + + S  +PLL+N+YPYFAY+
Sbjct: 160 LAKKIKVSTAIDTGVLGETFPPSIGSFKSEYNALLYPIIRFLVSHQSPLLVNLYPYFAYS 219

Query: 200 SNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETGWP 259
            N Q I L+YALFT+ + +V DGN  Y NLFDAM+D  YAALEK G  ++ + ISETGWP
Sbjct: 220 GNTQDIRLDYALFTAPSVVVQDGNFGYRNLFDAMLDGVYAALEKAGGGSLKVVISETGWP 279

Query: 260 TKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNFG 319
           +      T+++NA T+  N + HV    GTPRRP       +F MF+E+ K   +E+++G
Sbjct: 280 SAAGTA-TTIDNARTFISNLIQHVKE--GTPRRPGRPIETYIFAMFDENRKTPELEKHWG 339

Query: 320 FFYPNMHPVY 329
            F P   P Y
Sbjct: 340 LFSPTKQPKY 345

[Close]

BLAST of MC00g0042 vs. NCBI nr
Match: XP_022141986.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 628 bits (1620), Expect = 2.59e-226
Identity = 316/319 (99.06%), Postives = 316/319 (99.06%), Query Frame = 0


Query: 11  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 70
           L L  GLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 80

Query: 71  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 130
           LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN
Sbjct: 81  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 140

Query: 131 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 190
           IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL
Sbjct: 141 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 200

Query: 191 NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL 250
           NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL
Sbjct: 201 NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL 260

Query: 251 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 310
           EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA
Sbjct: 261 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 320

Query: 311 AGIEQNFGFFYPNMHPVYP 329
           AGIEQNFGFFYPNMHPVYP
Sbjct: 321 AGIEQNFGFFYPNMHPVYP 339

[Close]

BLAST of MC00g0042 vs. NCBI nr
Match: XP_022152302.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 556 bits (1434), Expect = 5.85e-198
Identity = 278/320 (86.88%), Postives = 294/320 (91.88%), Query Frame = 0


Query: 11  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 70
           L L  GLGGIGVNYGLNGDNLPQP+DVI+LYGRCGIDIVRIFEPNHGVLDA+ GKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVRIFEPNHGVLDALHGKPNALW 80

Query: 71  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 130
           LGTRNEDI SFA +Q AANAWVNA+V+PYYADVNI YITVGNEV+PADPA PFVANAIRN
Sbjct: 81  LGTRNEDIHSFATDQMAANAWVNAHVLPYYADVNIEYITVGNEVVPADPATPFVANAIRN 140

Query: 131 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 190
           IMQAL   G+KK IKVTTVV M AMGVSYPPSAG FT  A GPLS+IAKVVGSSG PLLL
Sbjct: 141 IMQALVTAGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAAAGPLSDIAKVVGSSGTPLLL 200

Query: 191 NVYPYFAYASNPQQISLNYALFTSTTP-IVDGNLHYYNLFDAMVDSFYAALEKIGATNVT 250
           NVYPYFAYAS+PQQI LNYALFTSTTP +VDGNL+YYNLFDAMVDSFYAALEKI A NV 
Sbjct: 201 NVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIDAANVR 260

Query: 251 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 310
           LEISETGWPTKGNEPFTSVENAVTYNKNF+NHV+SGAGTPRRPNVKY+GILFEMFNEDLK
Sbjct: 261 LEISETGWPTKGNEPFTSVENAVTYNKNFMNHVASGAGTPRRPNVKYNGILFEMFNEDLK 320

Query: 311 AAGIEQNFGFFYPNMHPVYP 329
           AAG+EQNFGFFYPNM PVYP
Sbjct: 321 AAGVEQNFGFFYPNMQPVYP 340

[Close]

BLAST of MC00g0042 vs. NCBI nr
Match: XP_022135630.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia])

HSP 1 Score: 543 bits (1400), Expect = 6.12e-193
Identity = 272/320 (85.00%), Postives = 292/320 (91.25%), Query Frame = 0


Query: 11  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 70
           L L  GLGGIGVNYGLNG+NLPQ NDVI+LYGRCGIDIVRIFEPNH VLDA+RGKPN LW
Sbjct: 11  LPLICGLGGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLW 70

Query: 71  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 130
           LGTRNEDI SFA +Q AANAWVN++VVPYYADVNIAYITVGNEVIP D AAPFVANAIRN
Sbjct: 71  LGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRN 130

Query: 131 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 190
           IMQAL +TGVK+ IKVTTVV MS +GVSYPPS G FT  A GPLS IAKVVGSSGAPLLL
Sbjct: 131 IMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLL 190

Query: 191 NVYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVT 250
           NVYPYFAYASNPQQI LNYALFTSTTP+V DGNL+YYNLFDAMVDSFYAALEKIGA +V 
Sbjct: 191 NVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVR 250

Query: 251 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 310
           LEISETGWPTKGN+PFTS++NAVTYNKNF+NHV+SGAGTP+RP VKY+GILFEMFNEDLK
Sbjct: 251 LEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLK 310

Query: 311 AAGIEQNFGFFYPNMHPVYP 329
            AG+EQNFGFFYPNM PVYP
Sbjct: 311 LAGVEQNFGFFYPNMQPVYP 330

[Close]

BLAST of MC00g0042 vs. NCBI nr
Match: XP_022935980.1 (probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata])

HSP 1 Score: 497 bits (1280), Expect = 1.70e-174
Identity = 242/319 (75.86%), Postives = 276/319 (86.52%), Query Frame = 0


Query: 13  LAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWL 72
           L  GLG +GVNYGLNGDNLP P++V+ LYGRCGIDI+R++EPNH VLDA++G+ N  +WL
Sbjct: 23  LISGLGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWL 82

Query: 73  GTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNI 132
           GTRNEDI  FA +Q AAN+WVNANVVPYY  VNIAYITVGNEV+P D AAPFVANAI+NI
Sbjct: 83  GTRNEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNI 142

Query: 133 MQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLN 192
           MQAL I GVK  IKVTTVVPMS + VSYPPSAG F+A A+GPL +I +V+G+SGAPLLLN
Sbjct: 143 MQALVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLN 202

Query: 193 VYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTL 252
            YPYFAYASNPQQISL+YALF ST P+V DGNL Y+NLFDAMVDSFY+ALEKIGA  V +
Sbjct: 203 AYPYFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRI 262

Query: 253 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 312
           +ISETGWP+KGN+PFTSV+NA TYNK FV HV SGAGTP+RPNVKY G+LFEMFNEDLK 
Sbjct: 263 DISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKT 322

Query: 313 AGIEQNFGFFYPNMHPVYP 329
           AG+EQNFG FYPNMH VYP
Sbjct: 323 AGVEQNFGMFYPNMHSVYP 341

[Close]

BLAST of MC00g0042 vs. NCBI nr
Match: XP_038896614.1 (glucan endo-1,3-beta-glucosidase-like [Benincasa hispida])

HSP 1 Score: 497 bits (1279), Expect = 2.78e-174
Identity = 247/317 (77.92%), Postives = 278/317 (87.70%), Query Frame = 0


Query: 15  GGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWLGT 74
           GG G +GVNYGLNGDNLP P+DVI+LYGRCGI+I+RIFEPNHGVLDA++G+ N  LWLGT
Sbjct: 29  GGSGTVGVNYGLNGDNLPIPSDVINLYGRCGINIIRIFEPNHGVLDALKGQANLVLWLGT 88

Query: 75  RNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQ 134
           RNEDI  FA +Q AANAWVNANVVPYY DVNIAYITVGNEV+PAD AAPFVANAI NIMQ
Sbjct: 89  RNEDIEGFATDQVAANAWVNANVVPYYKDVNIAYITVGNEVVPADAAAPFVANAITNIMQ 148

Query: 135 ALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVY 194
           AL   G++  IKVTTVV M+A+ VSYPPSAG FTA A G + +IA V+GSSGAP+L+NVY
Sbjct: 149 ALVNAGIQSDIKVTTVVAMTALEVSYPPSAGAFTATAAGVMKDIANVLGSSGAPILVNVY 208

Query: 195 PYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEI 254
           PYFAYASNP+QISLNYALFTS+TP+V DG+LHY+NLFDAMVDSFYAALEKI A  V + I
Sbjct: 209 PYFAYASNPEQISLNYALFTSSTPVVVDGDLHYFNLFDAMVDSFYAALEKIDAGGVGIAI 268

Query: 255 SETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAG 314
           SETGWPT GNEPFTSV+NA+TYNKNFV HV+SG GTP+RPNV Y  ILFEMFNEDLKA G
Sbjct: 269 SETGWPTDGNEPFTSVDNALTYNKNFVKHVTSGVGTPKRPNVFYDAILFEMFNEDLKAPG 328

Query: 315 IEQNFGFFYPNMHPVYP 329
           +EQNFGFFYPNM+PVYP
Sbjct: 329 VEQNFGFFYPNMNPVYP 345

[Close]

BLAST of MC00g0042 vs. ExPASy TrEMBL
Match: A0A6J1CKU8 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 PE=3 SV=1)

HSP 1 Score: 628 bits (1620), Expect = 1.25e-226
Identity = 316/319 (99.06%), Postives = 316/319 (99.06%), Query Frame = 0


Query: 11  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 70
           L L  GLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 80

Query: 71  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 130
           LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN
Sbjct: 81  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 140

Query: 131 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 190
           IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL
Sbjct: 141 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 200

Query: 191 NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL 250
           NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL
Sbjct: 201 NVYPYFAYASNPQQISLNYALFTSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTL 260

Query: 251 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 310
           EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA
Sbjct: 261 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 320

Query: 311 AGIEQNFGFFYPNMHPVYP 329
           AGIEQNFGFFYPNMHPVYP
Sbjct: 321 AGIEQNFGFFYPNMHPVYP 339

[Close]

BLAST of MC00g0042 vs. ExPASy TrEMBL
Match: A0A6J1DFU0 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 PE=3 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 2.83e-198
Identity = 278/320 (86.88%), Postives = 294/320 (91.88%), Query Frame = 0


Query: 11  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 70
           L L  GLGGIGVNYGLNGDNLPQP+DVI+LYGRCGIDIVRIFEPNHGVLDA+ GKPNALW
Sbjct: 21  LPLISGLGGIGVNYGLNGDNLPQPSDVINLYGRCGIDIVRIFEPNHGVLDALHGKPNALW 80

Query: 71  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 130
           LGTRNEDI SFA +Q AANAWVNA+V+PYYADVNI YITVGNEV+PADPA PFVANAIRN
Sbjct: 81  LGTRNEDIHSFATDQMAANAWVNAHVLPYYADVNIEYITVGNEVVPADPATPFVANAIRN 140

Query: 131 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 190
           IMQAL   G+KK IKVTTVV M AMGVSYPPSAG FT  A GPLS+IAKVVGSSG PLLL
Sbjct: 141 IMQALVTAGIKKDIKVTTVVHMGAMGVSYPPSAGVFTVAAAGPLSDIAKVVGSSGTPLLL 200

Query: 191 NVYPYFAYASNPQQISLNYALFTSTTP-IVDGNLHYYNLFDAMVDSFYAALEKIGATNVT 250
           NVYPYFAYAS+PQQI LNYALFTSTTP +VDGNL+YYNLFDAMVDSFYAALEKI A NV 
Sbjct: 201 NVYPYFAYASSPQQIPLNYALFTSTTPGVVDGNLNYYNLFDAMVDSFYAALEKIDAANVR 260

Query: 251 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 310
           LEISETGWPTKGNEPFTSVENAVTYNKNF+NHV+SGAGTPRRPNVKY+GILFEMFNEDLK
Sbjct: 261 LEISETGWPTKGNEPFTSVENAVTYNKNFMNHVASGAGTPRRPNVKYNGILFEMFNEDLK 320

Query: 311 AAGIEQNFGFFYPNMHPVYP 329
           AAG+EQNFGFFYPNM PVYP
Sbjct: 321 AAGVEQNFGFFYPNMQPVYP 340

[Close]

BLAST of MC00g0042 vs. ExPASy TrEMBL
Match: A0A6J1C1J7 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 PE=3 SV=1)

HSP 1 Score: 543 bits (1400), Expect = 2.96e-193
Identity = 272/320 (85.00%), Postives = 292/320 (91.25%), Query Frame = 0


Query: 11  LCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 70
           L L  GLGGIGVNYGLNG+NLPQ NDVI+LYGRCGIDIVRIFEPNH VLDA+RGKPN LW
Sbjct: 11  LPLICGLGGIGVNYGLNGNNLPQANDVINLYGRCGIDIVRIFEPNHYVLDALRGKPNVLW 70

Query: 71  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 130
           LGTRNEDI SFA +Q AANAWVN++VVPYYADVNIAYITVGNEVIP D AAPFVANAIRN
Sbjct: 71  LGTRNEDIQSFAADQLAANAWVNSHVVPYYADVNIAYITVGNEVIPGDAAAPFVANAIRN 130

Query: 131 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 190
           IMQAL +TGVK+ IKVTTVV MS +GVSYPPS G FT  A GPLS IAKVVGSSGAPLLL
Sbjct: 131 IMQALVVTGVKRDIKVTTVVHMSVIGVSYPPSTGAFTDAAAGPLSEIAKVVGSSGAPLLL 190

Query: 191 NVYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVT 250
           NVYPYFAYASNPQQI LNYALFTSTTP+V DGNL+YYNLFDAMVDSFYAALEKIGA +V 
Sbjct: 191 NVYPYFAYASNPQQIPLNYALFTSTTPVVVDGNLNYYNLFDAMVDSFYAALEKIGAVDVR 250

Query: 251 LEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLK 310
           LEISETGWPTKGN+PFTS++NAVTYNKNF+NHV+SGAGTP+RP VKY+GILFEMFNEDLK
Sbjct: 251 LEISETGWPTKGNQPFTSIDNAVTYNKNFMNHVASGAGTPKRPKVKYNGILFEMFNEDLK 310

Query: 311 AAGIEQNFGFFYPNMHPVYP 329
            AG+EQNFGFFYPNM PVYP
Sbjct: 311 LAGVEQNFGFFYPNMQPVYP 330

[Close]

BLAST of MC00g0042 vs. ExPASy TrEMBL
Match: A0A6J1FC84 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 PE=3 SV=1)

HSP 1 Score: 497 bits (1280), Expect = 8.21e-175
Identity = 242/319 (75.86%), Postives = 276/319 (86.52%), Query Frame = 0


Query: 13  LAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWL 72
           L  GLG +GVNYGLNGDNLP P++V+ LYGRCGIDI+R++EPNH VLDA++G+ N  +WL
Sbjct: 23  LISGLGAVGVNYGLNGDNLPVPSEVVGLYGRCGIDILRVYEPNHSVLDALKGRRNLVVWL 82

Query: 73  GTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNI 132
           GTRNEDI  FA +Q AAN+WVNANVVPYY  VNIAYITVGNEV+P D AAPFVANAI+NI
Sbjct: 83  GTRNEDIQGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNI 142

Query: 133 MQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLN 192
           MQAL I GVK  IKVTTVVPMS + VSYPPSAG F+A A+GPL +I +V+G+SGAPLLLN
Sbjct: 143 MQALVIAGVKSDIKVTTVVPMSVLAVSYPPSAGAFSATAIGPLKDITQVLGTSGAPLLLN 202

Query: 193 VYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTL 252
            YPYFAYASNPQQISL+YALF ST P+V DGNL Y+NLFDAMVDSFY+ALEKIGA  V +
Sbjct: 203 AYPYFAYASNPQQISLDYALFKSTAPVVVDGNLKYFNLFDAMVDSFYSALEKIGAEGVRI 262

Query: 253 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 312
           +ISETGWP+KGN+PFTSV+NA TYNK FV HV SGAGTP+RPNVKY G+LFEMFNEDLK 
Sbjct: 263 DISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNEDLKT 322

Query: 313 AGIEQNFGFFYPNMHPVYP 329
           AG+EQNFG FYPNMH VYP
Sbjct: 323 AGVEQNFGMFYPNMHSVYP 341

[Close]

BLAST of MC00g0042 vs. ExPASy TrEMBL
Match: A0A6J1IM63 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111476518 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 2.73e-173
Identity = 240/319 (75.24%), Postives = 276/319 (86.52%), Query Frame = 0


Query: 13  LAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWL 72
           L  GLG +GVNYGLNGDNLP P++V+ LYGRCGIDI+R++EPNH VLDA++G+ N  +WL
Sbjct: 23  LISGLGAVGVNYGLNGDNLPVPSEVVELYGRCGIDILRVYEPNHSVLDALKGRRNLVVWL 82

Query: 73  GTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNI 132
           GTRNEDI  FA +Q AAN+WVNANVVPYY  VNIAYITVGNEV+P D AAPFVANAI+NI
Sbjct: 83  GTRNEDIKGFAADQLAANSWVNANVVPYYKSVNIAYITVGNEVVPDDAAAPFVANAIKNI 142

Query: 133 MQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLN 192
           MQAL I GVK  IKV+TVVPMSA+ VS+PPSAG F+  A+GPL +I  V+G+SGAPLLLN
Sbjct: 143 MQALVIAGVKSDIKVSTVVPMSALAVSHPPSAGAFSTTAIGPLKDITLVLGTSGAPLLLN 202

Query: 193 VYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTL 252
            Y YFAYASNPQQISL+YALFTST P+V DGNL Y+NLFDAMVDSFY+ALEKIGA  V +
Sbjct: 203 AYTYFAYASNPQQISLDYALFTSTAPVVVDGNLQYFNLFDAMVDSFYSALEKIGAEGVRI 262

Query: 253 EISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKA 312
           +ISETGWP+KGN+PFTSV+NA TYNK FV HV SGAGTP+RPNVKY G+LFEMFNE+LK 
Sbjct: 263 DISETGWPSKGNDPFTSVDNAATYNKKFVEHVRSGAGTPKRPNVKYDGVLFEMFNENLKT 322

Query: 313 AGIEQNFGFFYPNMHPVYP 329
           AG+EQNFG FYPNMHPVYP
Sbjct: 323 AGVEQNFGMFYPNMHPVYP 341

[Close]

BLAST of MC00g0042 vs. TAIR 10
Match: AT5G20330.1 (beta-1,3-glucanase 4 )

HSP 1 Score: 281.2 bits (718), Expect = 1.1e-75
Identity = 148/312 (47.44%), Postives = 209/312 (66.99%), Query Frame = 0


Query: 20  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRG-KPNALWLGTRNEDI 79
           IG+NYGL GDNLP P++VI+LY   GI  +RIF+PN  VL+A+RG +   + +G +++D+
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 80  PSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAIT 139
            + A ++ A   W  AN+  Y ADVNI +ITVGNEVIP  P  P V   ++++   +   
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPG-PIGPQVLPVMQSLTNLVKSR 153

Query: 140 GVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFAY 199
            +   I ++TVV MS +  SYPPSAG FT+ A   L  + K++  +  P+L+N+YPYFAY
Sbjct: 154 NL--PISISTVVAMSNLEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAY 213

Query: 200 ASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETGW 259
           AS+P  I L+YA F + + +V DG+L Y N+FDA+ D+F  A+EK G  N+ + +SETGW
Sbjct: 214 ASDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGW 273

Query: 260 PTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNF 319
           P+ GN  FT+   A TYN+NFV H++SG GTP+RPN   +G LF  FNE+ K AG EQNF
Sbjct: 274 PSAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNF 333

Query: 320 GFFYP-NMHPVY 329
           G + P +M P+Y
Sbjct: 334 GLYNPSDMKPIY 342

[Close]

BLAST of MC00g0042 vs. TAIR 10
Match: AT1G77790.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 2.3e-73
Identity = 154/321 (47.98%), Postives = 203/321 (63.24%), Query Frame = 0


Query: 12  CLAGGLGGI-GVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNALW 71
           C AG  G I G  YG NG+NLP P D ++LY    ID +R++EP   +L+A+RG    + 
Sbjct: 21  CTAGVAGDITGDCYGRNGNNLPTPADTVALYKSNNIDAIRMYEPFADMLEALRGSGLLVA 80

Query: 72  LGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRN 131
            G RNEDI S A +  AA  +V+  + PY  DV I +IT+GNEV P +  A FVA AI+N
Sbjct: 81  FGPRNEDIQSLAHDPAAATNFVSTWITPYQNDVAIKWITIGNEVFPGE-IAQFVAAAIKN 140

Query: 132 IMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLL 191
           +  AL  +GV   I VTTV+ M+A+  +YPPSA TF       ++ I  ++  + +PL+ 
Sbjct: 141 VNVALTNSGV-TGISVTTVLAMTALTNTYPPSAATFLPDLTEIMTEITSILSETNSPLMT 200

Query: 192 NVYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNVT 251
           N+YPYFAYAS+P  ISL+YA F S TP+V DG+L+Y N+F+AMVD F AALEKI A NV 
Sbjct: 201 NIYPYFAYASDPYHISLDYASFKSNTPVVIDGDLYYNNMFEAMVDGFNAALEKINAANVV 260

Query: 252 LEISETGWPTKGNEPFTSVENAVTYNKNF--VNHVSSGAGTPRRPNVKYSGILFEMFNED 311
           + ++ETGWPT+GN P TSV+NA  YN         +    TPRR N      LF MF E+
Sbjct: 261 VMVAETGWPTEGNPPHTSVDNAKAYNMGIRTCGRSAERKRTPRRQNTPVDVFLFAMFKEN 320

Query: 312 LKAAGIEQNFGFFYPNMHPVY 329
            K   +EQ+FG F P+M PVY
Sbjct: 321 QKDGPVEQSFGIFAPDMTPVY 339

[Close]

BLAST of MC00g0042 vs. TAIR 10
Match: AT1G77780.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-73
Identity = 148/320 (46.25%), Postives = 202/320 (63.12%), Query Frame = 0


Query: 11  LCLAGGLGGI-GVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPNAL 70
           L  AG +    GV YG NGDNLP P   +SLY +  +  +R++EP   ++ +++G    +
Sbjct: 14  LTTAGNMNSFAGVCYGRNGDNLPSPAKTVSLYKKINVGGIRLYEPFPDLIVSLQGTGLLV 73

Query: 71  WLGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIR 130
            +G RNE I + A     A  W    + P Y +V   +ITVGNEVI  +    +V  A++
Sbjct: 74  AIGPRNEAIKTLAEEYQFALNWDKTFIAP-YKNVAFNWITVGNEVIEGE-IGRYVPQAMK 133

Query: 131 NIMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLL 190
           NI  AL   G  K I VTTV+  +A+  SYPPSAG F       ++ I  ++ S+ +PL+
Sbjct: 134 NIKAALTEIGNSK-IHVTTVISTAALANSYPPSAGVFKPAITELITEIVSILSSTDSPLM 193

Query: 191 LNVYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALEKIGATNV 250
           +NVYPYFAYAS+P  +SL YA F ST+P+V DG   Y N+FDA +D+F  ALEKI   +V
Sbjct: 194 VNVYPYFAYASDPSHVSLEYATFRSTSPVVTDGKYQYTNIFDATLDAFNVALEKINHGSV 253

Query: 251 TLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDL 310
            + ++ETGWPT+GN+P+TSVENA  YN+  +  +++G GTPRRPNV      FEMFNEDL
Sbjct: 254 KVYVAETGWPTRGNDPYTSVENARAYNQGLLKKLTTGKGTPRRPNVPVITFFFEMFNEDL 313

Query: 311 KAAGIEQNFGFFYPNMHPVY 329
           K   +EQ+FGFF PNM PVY
Sbjct: 314 KQGAVEQSFGFFDPNMAPVY 330

[Close]

BLAST of MC00g0042 vs. TAIR 10
Match: AT5G20340.1 (beta-1,3-glucanase 5 )

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-72
Identity = 143/328 (43.60%), Postives = 209/328 (63.72%), Query Frame = 0


Query: 4   YLNMLFCLCLAGGLGGIGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMR 63
           ++N    +        IG+NYGL GDNLP P+ VI+LY    I  +RIF+PN  VL+A+R
Sbjct: 27  FVNAANSIGFVNAANSIGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALR 86

Query: 64  G-KPNALWLGTRNEDIPSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAP 123
           G +  A+ +G R++D+ + + ++ A   W   N+ PY +D+NIA+ITVGNEVIP  P  P
Sbjct: 87  GHRDIAVTVGVRDQDLAALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPG-PIGP 146

Query: 124 FVANAIRNIMQALAITGVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVG 183
            V   ++++   +    +   I ++TVV M  +  SYPPSAG FT+ A   L  + K++ 
Sbjct: 147 QVLPVMQSLTNLVKSRNL--PISISTVVAMWNLEQSYPPSAGMFTSQAREQLVPVLKLLS 206

Query: 184 SSGAPLLLNVYPYFAYASNPQQISLNYALFTSTTPIV-DGNLHYYNLFDAMVDSFYAALE 243
            + +P+L+ +YPYF+YAS+P  I L+YA F +   +V DG+L Y N+FDA+ D+F  A+E
Sbjct: 207 QTNSPILVKIYPYFSYASDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAME 266

Query: 244 KIGATNVTLEISETGWPTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILF 303
           K G  ++ + +SETGWP+ GN   T+ + A TYN+NFV H++SG GTP+RPN    G LF
Sbjct: 267 KEGVKDLPMVVSETGWPSAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLF 326

Query: 304 EMFNEDLKAAGIEQNFGFFYPN-MHPVY 329
             FNE+ K  G EQNFG + PN M P+Y
Sbjct: 327 ATFNENQKPVGTEQNFGLYNPNDMKPIY 351

[Close]

BLAST of MC00g0042 vs. TAIR 10
Match: AT1G33220.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 6.8e-70
Identity = 140/311 (45.02%), Postives = 197/311 (63.34%), Query Frame = 0


Query: 20  IGVNYGLNGDNLPQPNDVISLYGRCGIDIVRIFEPNHGVLDAMRGKPN-ALWLGTRNEDI 79
           IG+NYGL GDN P P++V++LY   GI  +RIF+P   VL+A+RG  N  + +G R++D+
Sbjct: 34  IGLNYGLLGDNFPTPSNVLNLYKSIGITKIRIFDPKTEVLNALRGHRNIEVTVGVRDQDL 93

Query: 80  PSFAINQTAANAWVNANVVPYYADVNIAYITVGNEVIPADPAAPFVANAIRNIMQALAIT 139
            + + N+ A   W   N+ PY ADVNIA+ITVGNEVIP  P  P V   ++++   + + 
Sbjct: 94  GALSANEEAVKGWFATNIEPYLADVNIAFITVGNEVIPG-PIGPQVLPVMQSL--TILVK 153

Query: 140 GVKKAIKVTTVVPMSAMGVSYPPSAGTFTAVALGPLSNIAKVVGSSGAPLLLNVYPYFAY 199
            +   I ++TVV MS +  SYPPSAG FT+ A   L  + K++  +  P+L+N+YPYF Y
Sbjct: 154 SMNLPISISTVVAMSNLEQSYPPSAGEFTSQAREQLVPVLKLLSQTSTPILVNIYPYFPY 213

Query: 200 ASNPQQISLNYALF-TSTTPIVDGNLHYYNLFDAMVDSFYAALEKIGATNVTLEISETGW 259
           AS+P  I L+YA F T  T + DG L Y N+FDA+ D+F  A+EK G  ++ + +SETGW
Sbjct: 214 ASDPTNIPLDYATFNTKATVVQDGPLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGW 273

Query: 260 PTKGNEPFTSVENAVTYNKNFVNHVSSGAGTPRRPNVKYSGILFEMFNEDLKAAGIEQNF 319
           P+ GN   T+ + A TYN+NFV H+ SG GTP+RPN    G LF  FNE+ K AG +   
Sbjct: 274 PSAGNGNLTTPDIAGTYNRNFVKHIVSGKGTPKRPNNGMDGFLFATFNENQKPAGTD--- 332

Query: 320 GFFYPNMHPVY 329
                 M P+Y
Sbjct: 334 ------MKPIY 332

The following BLAST results are available for this feature:

BLAST of MC00g0042 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22317	2.9e-81	49.68	Glucan endo-1,3-beta-glucosidase OS=Musa acuminata OX=4641 GN=BANGLUC PE=1 SV=2	[more]
O49352	1.5e-74	47.44	Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O49353	2.1e-71	43.60	Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q02439	8.1e-68	45.25	Putative glucan endo-1,3-beta-glucosidase GVI (Fragment) OS=Hordeum vulgare OX=4...	[more]
P52408	2.4e-64	43.87	Glucan endo-1,3-beta-glucosidase, basic isoform OS=Prunus persica OX=3760 GN=GNS...	[more]

BLAST of MC00g0042 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141986.1	2.59e-226	99.06	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022152302.1	5.85e-198	86.88	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022135630.1	6.12e-193	85.00	probable glucan endo-1,3-beta-glucosidase BG4 [Momordica charantia]	[more]
XP_022935980.1	1.70e-174	75.86	probable glucan endo-1,3-beta-glucosidase BG4 [Cucurbita moschata]	[more]
XP_038896614.1	2.78e-174	77.92	glucan endo-1,3-beta-glucosidase-like [Benincasa hispida]	[more]

BLAST of MC00g0042 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKU8	1.25e-226	99.06	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012224 ...	[more]
A0A6J1DFU0	2.83e-198	86.88	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111020050 ...	[more]
A0A6J1C1J7	2.96e-193	85.00	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111007535 ...	[more]
A0A6J1FC84	8.21e-175	75.86	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111442718 P...	[more]
A0A6J1IM63	2.73e-173	75.24	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111476518 PE=...	[more]

BLAST of MC00g0042 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20330.1	1.1e-75	47.44	beta-1,3-glucanase 4	[more]
AT1G77790.1	2.3e-73	47.98	Glycosyl hydrolase superfamily protein	[more]
AT1G77780.1	5.0e-73	46.25	Glycosyl hydrolase superfamily protein	[more]
AT5G20340.1	1.5e-72	43.60	beta-1,3-glucanase 5	[more]
AT1G33220.1	6.8e-70	45.02	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 20..328 e-value: 5.8E-88 score: 295.5
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 19..329 e-value: 7.5E-112 score: 375.4
None	No IPR available	PANTHER	PTHR32227:SF248	GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN	coord: 14..328
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 14..328
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 20..329

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0042.1	MC00g0042.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds