MC00g0016 (gene) Bitter gourd (Dali-11) v1

Overview


Name	MC00g0016
Type	gene
Organism	Momordica charantia cv. Dali-11 (Bitter gourd (Dali-11) v1)
Description	Nitronate monooxygenase
Location	scaffold31: 214232 .. 218842 (+)
RNA-Seq Expression	MC00g0016
Synteny	MC00g0016

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: utr5CDSpolypeptideutr3

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGGACCAAGAAAAACGATTCTTTTAATAGATTAAACACGTGCTGCCCTAAAAAAAAATAACAAAACAGAGAGGAAGAATTGTTCACGTCGCCGCATTACGTCGTCGTATCGCACTACCTGACGGGCTAAAGCGATTGTGCGGCGTATTTGCTTACGTGTTGAACCCGGCAGGTCTCACGTAACCTTCCAAGCCGCCTCCCATGGCCGCCACTGCCGCTTTCAACTTCCAATATATTCTCGCTTCTTCCGATCAATCCATTGCCATTGCCAGCGGAAGAGAGAAAGACGCATGGCTTTCGGAGGGATTCTTGGTTTCCAGTACGGCATAGTTCAGGCTCCACTTGGACCTGATATCTCCAGTCCCGAGCTTGTTGCTGCCGTCGCCAATGCCGGTGGACTCGGCCTCCTCAGAGCCCCCGATTGGGTATCTTTTTCTATACTATTTCTTCTTTCTGCCTTTAGATGTACCGCAAAATGGAAAAAATGCTCGTTGAATGTAACGTGTTGTGGAGAAGCATCATCTGATCTGACGAAAATTGAGCAGAAACGGAATTTCTGTTCCTAATTAGTTCAGTGATTGATTCTTATCTCCGTGTGATGTCTGATTGTTCGATCATACGTACGTCCTGTTCGGATTTTGAAACTTCAATTTTTTAATCGTCCTTTTTATTTTACTACTGGTTATTGCATGGTTTTATCGGAGTGCAGACAATAAAACGATAACTGGTTGCGGAATTCTGGGGGATTTTTGAGTTAACTGAACATTTGAAATATTGATCTCGTCAATGCGTCATTGTGAACAGTTCGTATATGGTCTGAATGTAAATAATTCACTGTAAGCATCGTATAGCTATTTATTTGATTAGTCTATAAATTAATACTAAAATTGAGCAAAGCATCGGTTTCGTAACTGTTTTGTTTTTTGTTGTCAAAGAGCTAGTGATGTATTTAGCTTAGTTTCTACTTTTTGTATTGGTTTTGGATTTTAGCCAAATTTTGAACAAGAAATGAGATTTTTGGAAACCACATGTTTTTGGTCTCTGAATTTTTCTGTTACCTCAAAATGATTATGGAATATTGATATGTGTAAAGTGCTGAACAAGCTTATCTTCTTAAATTGAAAACATATAATACAGTGATTATCGAACAGTCTTTTATTATAAAAGTCAAGAGGTGTATAGTTGTATGGAGCTCGTACATTTAAAGCTTTTTATATATATTTATATGTACCAGGAGTCACCTGATTTTATTCGAGAATTGATAAGGAAGACCCGAGCTTTAACAGAAAAACCATTTGGAGTTGGTGTCATTTTAGCATTTCCTCATGAGCAGAATTTAAGGGCCATATTAGATGAAAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAGTGTTCAAAGGATCTTGTAGATCAGGTTCATTCTGCTGGGGTGAAAATTATTCCACAAGTGAGTGTTCTCTTGCAAATCACATTAACTGTACATGTCCAAAAGGTTCAATTCAATGGTTGATTGCTTTGTCTCATCATTATATTTTTATGCACGGTTGCTAGTGGCTTTAAGAACTACTTTTGCAGGTTGGGAGTGTTGAAGAAGCGAGAAAAGCAGTTGATGTTGGTGTTGATGCAATAATTGTCCAAGGACGGGAAGCAGGAGGACATGTTATTGGTCAAGTATAGATCTTCTTCTTCCTTGAAAACTTAATTAATTCGCGATGCTATATATTTGTTTCTAGATTCTTCCATTATAATCCGTTTAATTTTTCTTTTCATTGTCTTAATCACTTGGGGGCCGTGGTTTTGTACGGCCCTTCTCCTTTGTAAAAAGTTCTCTAAATTACTAGGAAAAGAAAAGAATTAACAGGTAAAATAATTTACATAAGACAGTTAATTCATCAAACGTCTGCTTTCAATTCACTTAGAGATTATTGTGTTAGTTCAGATGCCTACCCAGGGTACCCAGTTACCCTGTTCTGTGTTCATCTTTTGAAGGATACAACATTTGTTTATTTGAGATAATGGTTATGTGATAGGTCTCAGTTGACCTGTCTCAGCTAATTCATTCACAAAAACAATAACACAATAAACAAAGTTCCTAGCACTTGGAGAGGCTAGGACTCTCTCACACACAAGTCTAAGAATACCAAAATACTACTAAAACAATATCCCTCCCTACTGGCCCGAACGCTGCTAAGACCCCTCCCCGCCAGGCCTATTGCTGTACAACCCTATTACAAAAATGGGGTCCCGCACGACACTACTAACTAACTTCTGCAGCTTTTTCTTTGTTCCTCCCTGGTGCCGTTTTGCCTTTGTGTGTACGTATAAAATACCTGAGGTGGCTCTTCTCTATCATTATGTTGGAAGCCTGTATTCAATTTGAAATCAACTCTGGAGAGAACTAGATTTTGAATATTGATCTTTATCAGTATGTATTTTGACATTTTTCCCTTTTGACATTAATCATGCATATATTATTGAAGTATTTCCCTTTTGAAATTAATCATGCATATATTTTTGAACTAAGTATATGAACACATGAACCTAGCAGTCTGCCAGGTTTGCTTTTTGCTGCCTCCGGTCGTATCACCTAGATTCATGTGTTCATTAATCATATTCTTCACCTCTCTTCACATTTATCTTTCAACTTTATCATAACGTTGCTCAAATAAATTGTCTATAGAAGTTTGACTTAGTTCTTTACAGGAAGGTTTGATTTCCATCTTACCAAGGGTAGTTGATATTGTTGGAAATGAAGATATACCTGTAATTGCGGCTGGTGGAATTTCAGATTCGCGAGGTTATGCTGCTGCTCTGGCCCTTGGTGCTCGTGGTGTCTGCCTTGGAACTAGGTTTGAAAAGCTCAACTGTGAATACAACTTACAGAACTTGTTTTTAAAAAACTGGTAGACCCGTGCTACATATTGGTGCTCAATTAGAAAGAATTTCCTAGTTGTAAGAATATTGCACATGTTCAGTCAGCGTTTGGTTCAGGTTCAGAGCCACCATATTAATGAACCCGTGTCCATGTTATGAAGGTAAGTATGGTAATAAAACTGCCCCGTGACACATATTCTTTAACATATGTCTAATTTTAGTTCATTTAAGCCATGAAAATGGCGTTGGAGAGAGTTTTATCGAGGTTGACTGGTCACATCGTAATCCATGTACTAAGTAGTGTTTTCATTTAGTGTAATCCAAAACTTGCAGTTATCCTCATATTATCGAACATGGAACGATTAAATAATTTAATGACGAAACATATCTCAAGAATTTTAGGTACTATCACTATTGATTTTTTTGCATCTCCTGAGATTTCAATTTTGCTTTTAGAAGTTTGTTGCTATATATAGTAATTTGAGCACCCAAGAACTAAATGAAATATTGCAGGTTCGTTGCTACTGAAGAAAGCAATGCTCACCCTACATACAAAAGGAAGTTGGTGGAAATGGAGGCAACCGATTATACAAACATTTTCGGCCGCGCAAGATGGCCCGATGCCCCACAACGTGTTCTACAGACGCCCTTTTATGATGATTGGAAGTCACTTCCAGCCCATGAAAATGAAAGCAACCAGCCTGTTATCGGTCGTTCAACAATTAATGGCCTGGTGAGCTCCCCTTACAATGTTCTTCTCCACTTAAACAGGTTTTATAAGAGGGATATGGAAACGCTGTAGTTACATAGTTCATTCAATGGACCATGAAGTAAAAAACATACTAGCGTTCAGCCACACTTTGAATTATATGTATCTTTTTCCCGCATATAATACATCCATGCTTGGACAGGATGTAGGTATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGGCAAAAGGCGACATCGAGAGCATGGCAATGTATGCTGGGCAAGGAGTTGGACTAATCAAGGAGATTCTACCCGCGGGAGAAGTGGTAAGGAGGCTGGTTGAAGGTGCTCAGCATCAGATCCAAACACATTTTAGAAGTTAACTTATGTTCATAAAGTTTGATGATGAGAGCCCCAAAATTTTTGGTTTTGCTGAACAAATAAGATCGAAAATGCATATGTAAGTTGGATGAATGGCTGAAAATTTGAGTTTCACTGTTAATCATGCACTCAGCAAACCTCAAGTTCTAATAAAGTAGTCTGTTCATAACAGTAGAATTTTAGGTCCTTATCCTTCAAGGTTGTGTAGAGGTTTTCGAGTTTCGGAAGGACATAGTAATGGTTCTGTTTCTCGTTTGATCGTAAAAACAAGTATCTTTTTTCCTCTAAAACTACATCTTTTACATTTGCCCTCAGCTTCTATCTTAGAGTATTTATTATTTACCATGTTTTCCATAATGCCAAATGAGCTTGTCCTCAATGTTTCTCGGTGGGTGGGTTACTCAAAATGGAATCAGCATCCATCAATCTGTATAAGGAACGTATTCATTGTGTGTATAACTTGAATGCAATGTTGTAATTATGATTATAGTTATAATTTGAATGCAAAGCCACTAATATAACGTTGAATAACATGTACGATGACCAAAAATAAATGTGTGAATATTTTGATTGCCGTCGTGAAATGTTCCACTTGCAGTTCGTTCAATCCCCCACCTCCACATGTGGGTTTTGTTCAATCCC

mRNA sequence

CCGGACCAAGAAAAACGATTCTTTTAATAGATTAAACACGTGCTGCCCTAAAAAAAAATAACAAAACAGAGAGGAAGAATTGTTCACGTCGCCGCATTACGTCGTCGTATCGCACTACCTGACGGGCTAAAGCGATTGTGCGGCGTATTTGCTTACGTGTTGAACCCGGCAGGTCTCACGTAACCTTCCAAGCCGCCTCCCATGGCCGCCACTGCCGCTTTCAACTTCCAATATATTCTCGCTTCTTCCGATCAATCCATTGCCATTGCCAGCGGAAGAGAGAAAGACGCATGGCTTTCGGAGGGATTCTTGGTTTCCAGTACGGCATAGTTCAGGCTCCACTTGGACCTGATATCTCCAGTCCCGAGCTTGTTGCTGCCGTCGCCAATGCCGGTGGACTCGGCCTCCTCAGAGCCCCCGATTGGGAGTCACCTGATTTTATTCGAGAATTGATAAGGAAGACCCGAGCTTTAACAGAAAAACCATTTGGAGTTGGTGTCATTTTAGCATTTCCTCATGAGCAGAATTTAAGGGCCATATTAGATGAAAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAGTGTTCAAAGGATCTTGTAGATCAGGTTCATTCTGCTGGGGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCGAGAAAAGCAGTTGATGTTGGTGTTGATGCAATAATTGTCCAAGGACGGGAAGCAGGAGGACATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGGTAGTTGATATTGTTGGAAATGAAGATATACCTGTAATTGCGGCTGGTGGAATTTCAGATTCGCGAGGTTATGCTGCTGCTCTGGCCCTTGGTGCTCGTGGTGTCTGCCTTGGAACTAGGTTCGTTGCTACTGAAGAAAGCAATGCTCACCCTACATACAAAAGGAAGTTGGTGGAAATGGAGGCAACCGATTATACAAACATTTTCGGCCGCGCAAGATGGCCCGATGCCCCACAACGTGTTCTACAGACGCCCTTTTATGATGATTGGAAGTCACTTCCAGCCCATGAAAATGAAAGCAACCAGCCTGTTATCGGTCGTTCAACAATTAATGGCCTGGATGTAGGTATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGGCAAAAGGCGACATCGAGAGCATGGCAATGTATGCTGGGCAAGGAGTTGGACTAATCAAGGAGATTCTACCCGCGGGAGAAGTGGTAAGGAGGCTGGTTGAAGGTGCTCAGCATCAGATCCAAACACATTTTAGAAGTTAACTTATGTTCATAAAGTTTGATGATGAGAGCCCCAAAATTTTTGGTTTTGCTGAACAAATAAGATCGAAAATGCATATGTAAGTTGGATGAATGGCTGAAAATTTGAGTTTCACTGTTAATCATGCACTCAGCAAACCTCAAGTTCTAATAAAGTAGTCTGTTCATAACAGTAGAATTTTAGGTCCTTATCCTTCAAGGTTGTGTAGAGGTTTTCGAGTTTCGGAAGGACATAGTAATGGTTCTGTTTCTCGTTTGATCGTAAAAACAAGTATCTTTTTTCCTCTAAAACTACATCTTTTACATTTGCCCTCAGCTTCTATCTTAGAGTATTTATTATTTACCATGTTTTCCATAATGCCAAATGAGCTTGTCCTCAATGTTTCTCGGTGGGTGGGTTACTCAAAATGGAATCAGCATCCATCAATCTGTATAAGGAACGTATTCATTGTGTGTATAACTTGAATGCAATGTTGTAATTATGATTATAGTTATAATTTGAATGCAAAGCCACTAATATAACGTTGAATAACATGTACGATGACCAAAAATAAATGTGTGAATATTTTGATTGCCGTCGTGAAATGTTCCACTTGCAGTTCGTTCAATCCCCCACCTCCACATGTGGGTTTTGTTCAATCCC

Coding sequence (CDS)

ATGGCTTTCGGAGGGATTCTTGGTTTCCAGTACGGCATAGTTCAGGCTCCACTTGGACCTGATATCTCCAGTCCCGAGCTTGTTGCTGCCGTCGCCAATGCCGGTGGACTCGGCCTCCTCAGAGCCCCCGATTGGGAGTCACCTGATTTTATTCGAGAATTGATAAGGAAGACCCGAGCTTTAACAGAAAAACCATTTGGAGTTGGTGTCATTTTAGCATTTCCTCATGAGCAGAATTTAAGGGCCATATTAGATGAAAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAGTGTTCAAAGGATCTTGTAGATCAGGTTCATTCTGCTGGGGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCGAGAAAAGCAGTTGATGTTGGTGTTGATGCAATAATTGTCCAAGGACGGGAAGCAGGAGGACATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGGTAGTTGATATTGTTGGAAATGAAGATATACCTGTAATTGCGGCTGGTGGAATTTCAGATTCGCGAGGTTATGCTGCTGCTCTGGCCCTTGGTGCTCGTGGTGTCTGCCTTGGAACTAGGTTCGTTGCTACTGAAGAAAGCAATGCTCACCCTACATACAAAAGGAAGTTGGTGGAAATGGAGGCAACCGATTATACAAACATTTTCGGCCGCGCAAGATGGCCCGATGCCCCACAACGTGTTCTACAGACGCCCTTTTATGATGATTGGAAGTCACTTCCAGCCCATGAAAATGAAAGCAACCAGCCTGTTATCGGTCGTTCAACAATTAATGGCCTGGATGTAGGTATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGGCAAAAGGCGACATCGAGAGCATGGCAATGTATGCTGGGCAAGGAGTTGGACTAATCAAGGAGATTCTACCCGCGGGAGAAGTGGTAAGGAGGCTGGTTGAAGGTGCTCAGCATCAGATCCAAACACATTTTAGAAGTTAA

Protein sequence

MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRALTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRGYAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQTPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHQIQTHFRS

Homology

[Close]

BLAST of MC00g0016 vs. ExPASy Swiss-Prot
Match: Q0S7Q1 ((3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=ipdC PE=3 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.5e-33
Identity = 94/239 (39.33%), Postives = 133/239 (55.65%), Query Frame = 0


Query: 6   ILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRALTEKP 65
           ++G Q+ +VQ  +G  ++ P LVA  ANAGGLG+L +      + +   I KT+ LT+KP
Sbjct: 11  LVGVQHPVVQTGMG-WVAGPRLVAGTANAGGLGILASATMTYTE-LESAITKTKTLTDKP 70

Query: 66  FGVGV---ILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEE 125
           FGV +       PH  +L  ++ E V V         ++L+ ++ +AGV +IP +G+ + 
Sbjct: 71  FGVNIRADASDAPHRIDL--LIRESVRVASFALAP-KQELIAKLKAAGVVVIPSIGAAKH 130

Query: 126 ARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRGYA 185
           A+K    G DA+IVQG E GGH  G      +LP V+D V   DIPV+AAGG  D RG A
Sbjct: 131 AKKVASWGADAVIVQGGEGGGHT-GPVATTLLLPSVLDAV---DIPVVAAGGFYDGRGLA 190

Query: 186 AALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQT 242
           AALA GA GV +GTRF+ T+ES    + K + +     D T      +    P RVL T
Sbjct: 191 AALAYGAAGVAMGTRFLLTQESTVPDSVKHEYLARGLQDTT---VSRKVDGMPHRVLNT 237

[Close]

BLAST of MC00g0016 vs. ExPASy Swiss-Prot
Match: P71847 ((3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ipdC PE=1 SV=3)

HSP 1 Score: 137.1 bits (344), Expect = 3.6e-31
Identity = 92/247 (37.25%), Postives = 133/247 (53.85%), Query Frame = 0


Query: 6   ILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRALTEKP 65
           ++G ++ +VQ  +G  ++   LV+A ANAGGLG+L A    + D +   I K +A+T+KP
Sbjct: 10  LIGIEHPVVQTGMG-WVAGARLVSATANAGGLGIL-ASATMTLDELAAAITKVKAVTDKP 69

Query: 66  FGVGVIL-AFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAR 125
           FGV +   A      +  ++ E V V         + L+ ++  AG  +IP +G+ + AR
Sbjct: 70  FGVNIRADAADAGDRVELMIREGVRVASFALAP-KQQLIARLKEAGAVVIPSIGAAKHAR 129

Query: 126 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRGYAAA 185
           K    G DA+IVQG E GGH  G      +LP V+D V    IPVIAAGG  D RG AAA
Sbjct: 130 KVAAWGADAMIVQGGEGGGHT-GPVATTLLLPSVLDAVAGTGIPVIAAGGFFDGRGLAAA 189

Query: 186 LALGARGVCLGTRFVATEESNAHPTYKRKLVE--MEATDYTNIFGRARWPDAPQRVLQTP 245
           L  GA GV +GTRF+ T +S      KR+ ++  ++ T  T      R    P RVL+T 
Sbjct: 190 LCYGAAGVAMGTRFLLTSDSTVPDAVKRRYLQAGLDGTVVT-----TRVDGMPHRVLRTE 247

Query: 246 FYDDWKS 250
             +  +S
Sbjct: 250 LVEKLES 247

[Close]

BLAST of MC00g0016 vs. ExPASy Swiss-Prot
Match: B2TEK6 (Nitronate monooxygenase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) OX=398527 GN=Bphyt_4144 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.6e-29
Identity = 117/367 (31.88%), Postives = 170/367 (46.32%), Query Frame = 0


Query: 6   ILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRALTEKP 65
           +LG    I+QAP+   +S+P L AAV NAGGLG L      + D  R++IR+TRALT+KP
Sbjct: 13  LLGIDKPIIQAPMA-GVSTPALAAAVCNAGGLGSLGVGAMNA-DGARKVIRETRALTDKP 72

Query: 66  FGVGVILAFPHEQN---------------------------------------LRAILDE 125
           F + V    P + +                                       L   L+E
Sbjct: 73  FNINVFCHRPAQADAAVEQQWLSWLAPHFEKYGATPPAKLSDIYTSFLADPAMLAVFLEE 132

Query: 126 KVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEARKAVDVGVDAIIVQGREAGGH-- 185
           K A+V  ++G  S D++ ++  AG++++    +++EA +    GVDAI+ QG EAGGH  
Sbjct: 133 KPAIVSFHFGLPSADVIAELKKAGIRLLASATNLQEAAQVEAAGVDAIVAQGIEAGGHRG 192

Query: 186 VIGQEGLISILP--RVVDIVGNE-DIPVIAAGGISDSRGYAAALALGARGVCLGTRFVAT 245
           V   +     L    +  ++  E  +PVIAAGGI D  G AAALALGA+   LGT FVA 
Sbjct: 193 VFDPDAFDDRLGTFALTRLLAKECRLPVIAAGGIMDGAGIAAALALGAQAAQLGTAFVAC 252

Query: 246 EESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQTPFYDDWKSLPAHENESNQPV 305
            E++    Y+R L+   A        R  +  A    L     + + +L A +  S +P 
Sbjct: 253 TETSIDEGYRRALLGEAAR-------RTTFTAAISGRLARSMANSFTALGA-DPRSPEPA 312

Query: 306 IGRSTINGLDVGIRRFAGTVPNAT--AKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVE 327
                       I   AG   NA   AKG+    A +AGQ   L +  LPA E+V +L  
Sbjct: 313 ---------TYPIAYDAGKALNAAAKAKGEFGYGAHWAGQAAALARS-LPAAELVAQLER 359

[Close]

BLAST of MC00g0016 vs. ExPASy Swiss-Prot
Match: Q9HWH9 (Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=nmoA PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.2e-28
Identity = 114/363 (31.40%), Postives = 163/363 (44.90%), Query Frame = 0


Query: 3   FGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRALT 62
           F  +LG Q  I+QAP+   +S+P L AAV+NAGGLG + A    + +  R LIR+ R LT
Sbjct: 5   FTRLLGIQQPIIQAPM-LGVSTPALAAAVSNAGGLGSI-AITGSAAEKGRALIREVRGLT 64

Query: 63  EKPFGVGVILAFPHEQN---------------------------------------LRAI 122
           +KPF V +    P + +                                       L  +
Sbjct: 65  DKPFNVNLFCHRPGQADPARERAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPML 124

Query: 123 LDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEARKAVDVGVDAIIVQGREAGG 182
           L+E+ A V  ++G   +D V  + + G++++    + EEA      G DA++ QG EAGG
Sbjct: 125 LEERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGG 184

Query: 183 H--VIGQE---GLISILPRVVDIVGNEDIPVIAAGGISDSRGYAAALALGARGVCLGTRF 242
           H  V   E     I  L  V  +     +PV+AAGGI D RG  AAL LGA  V +GT F
Sbjct: 185 HRGVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAF 244

Query: 243 VATEESNAHPTYKRKLVEMEA--TDYTNIFGRARWPDAPQRVLQTPFYDDWKSLPAHENE 302
           V   ES+A+  Y+  L    A  T  T           P R+    F+D   + P     
Sbjct: 245 VLCPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRM----FFD--AAAPGVPPL 304

Query: 303 SNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQGVGLIKEILPAGEVVRR 320
            + P +  +T                 A A+G+ +  A +AGQG  L +E LPA E++R 
Sbjct: 305 PDYPFVYDAT-----------KALQTAALARGNHDFAAQWAGQGAALARE-LPAAELLRT 347

[Close]

BLAST of MC00g0016 vs. ExPASy Swiss-Prot
Match: Q01284 (Nitronate monooxygenase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ncd-2 PE=1 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.8e-27
Identity = 105/364 (28.85%), Postives = 168/364 (46.15%), Query Frame = 0


Query: 13  IVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPD------FIREL--IRKTRALTEK 72
           I+ AP+   I++  L A V+ AGG+G +       P          EL   R    LT++
Sbjct: 33  IISAPMYL-IANGTLAAEVSKAGGIGFVAGGSDFRPGSSHLTALSTELASARSRLGLTDR 92

Query: 73  PF----GVGVILAFPHEQNLRAILDEKVAVV-----QVYW----------------GECS 132
           P     G+GV L   H  ++  + D  + ++     Q  W                   +
Sbjct: 93  PLTPLPGIGVGLILTHTISVPYVTDTVLPILIEHSPQAVWLFANDPDFEASSEPGAKGTA 152

Query: 133 KDLVDQVHSAGVKIIPQVGSVEEARKAVDVGVDAIIVQGREAGGHVIGQ-EGLISILPRV 192
           K +++ +H++G  +  QVG+V++ARKA   G D I+ QG +AGGH +    G++S++P V
Sbjct: 153 KQIIEALHASGFVVFFQVGTVKDARKAAADGADVIVAQGIDAGGHQLATGSGIVSLVPEV 212

Query: 193 VDIVGNE----DIPVIAAGGISDSRGYAAALALGARGVCLGTRFVATEESNAHPTYKRKL 252
            D++  E    ++ V+AAGG++D RG   AL LGA GV LGTRF    E++  P ++RK+
Sbjct: 213 RDMLDREFKEREVVVVAAGGVADGRGVVGALGLGAEGVVLGTRFTVAVEAST-PEFRRKV 272

Query: 253 VEMEATD----------YTNIFGRARWPDA-PQRVLQTPFYDDWKS-LPAHENESNQPVI 312
           + +E  D          +  I     W +    R ++   YDD  + +P  EN       
Sbjct: 273 I-LETNDGGLNTVKSHFHDQINCNTIWHNVYDGRAVRNASYDDHAAGVPFEENHKK---- 332

Query: 313 GRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQ 327
                                A + GD      ++G  VGLIK+  PAG++VR L E A+
Sbjct: 333 ------------------FKEAASSGDNSRAVTWSGTAVGLIKDQRPAGDIVRELREEAK 371

[Close]

BLAST of MC00g0016 vs. NCBI nr
Match: XP_022141996.1 (uncharacterized protein LOC111012234 isoform X1 [Momordica charantia])

HSP 1 Score: 656 bits (1692), Expect = 1.90e-237
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA
Sbjct: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG
Sbjct: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ
Sbjct: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331

[Close]

BLAST of MC00g0016 vs. NCBI nr
Match: XP_008455173.1 (PREDICTED: probable nitronate monooxygenase [Cucumis melo] >KAA0031461.1 putative nitronate monooxygenase [Cucumis melo var. makuwa] >TYK06915.1 putative nitronate monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 620 bits (1598), Expect = 4.87e-223
Identity = 309/331 (93.35%), Postives = 320/331 (96.68%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD++RELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+KPFG+GVILAFPHEQNL+AILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVD GVDAIIVQGREAGGHVIGQEGLISILPRVV+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEES+AHPTYKRKLVEMEATDYTNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ IRRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQH IQTH  S
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSS 331

[Close]

BLAST of MC00g0016 vs. NCBI nr
Match: XP_031744639.1 (uncharacterized protein LOC101221123 [Cucumis sativus])

HSP 1 Score: 617 bits (1590), Expect = 8.07e-222
Identity = 307/331 (92.75%), Postives = 320/331 (96.68%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD++RELIRKTRA
Sbjct: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+KPFG+GVILAFPHEQNL+AILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+IVG++DIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEESNAHPTYKRKLVE+EATDYTNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ IRRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQH I+TH  S
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSS 331

[Close]

BLAST of MC00g0016 vs. NCBI nr
Match: XP_038888874.1 (nitronate monooxygenase isoform X1 [Benincasa hispida])

HSP 1 Score: 615 bits (1586), Expect = 3.28e-221
Identity = 311/331 (93.96%), Postives = 316/331 (95.47%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M F GILGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDF+R LIRKTRA
Sbjct: 1   MDFRGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDFVRGLIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+K FGVGVILAFPHEQNL+AILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKTFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVD GVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEES AHPTYKRKLVE+EATDYTNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLDV IRRFAGTVPN T KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDVDIRRFAGTVPNETTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQH IQTHF S
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHFSS 331

[Close]

BLAST of MC00g0016 vs. NCBI nr
Match: KAG6588765.1 (hypothetical protein SDJN03_17330, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 610 bits (1574), Expect = 2.05e-219
Identity = 302/331 (91.24%), Postives = 319/331 (96.37%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M+FGGILGF+YG+VQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD++RELIRKTRA
Sbjct: 1   MSFGGILGFEYGVVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT KPFGVG+ILAFPHEQNLRAIL EKVAVVQVYWGECSKDLVDQVHSAGVKIIPQ+GSV
Sbjct: 61  LTNKPFGVGIILAFPHEQNLRAILAEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQIGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEARKAVDVGVDA+IVQGREAGGHVIGQEGLISILP VVDIVG++DIPVIAAGGISDSRG
Sbjct: 121 EEARKAVDVGVDAVIVQGREAGGHVIGQEGLISILPGVVDIVGDKDIPVIAAGGISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVAT+ES+AHP YKRKLVEM+ATD+TNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATKESHAHPAYKRKLVEMDATDHTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ IRRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEI+PAGEVVR LVEGAQH IQTHF S
Sbjct: 301 VGLIKEIIPAGEVVRMLVEGAQHHIQTHFSS 331

[Close]

BLAST of MC00g0016 vs. ExPASy TrEMBL
Match: A0A6J1CKV8 (uncharacterized protein LOC111012234 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012234 PE=4 SV=1)

HSP 1 Score: 656 bits (1692), Expect = 9.20e-238
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA
Sbjct: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG
Sbjct: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ
Sbjct: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331

[Close]

BLAST of MC00g0016 vs. ExPASy TrEMBL
Match: A0A5A7SPI9 (Putative nitronate monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002230 PE=4 SV=1)

HSP 1 Score: 620 bits (1598), Expect = 2.36e-223
Identity = 309/331 (93.35%), Postives = 320/331 (96.68%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD++RELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+KPFG+GVILAFPHEQNL+AILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVD GVDAIIVQGREAGGHVIGQEGLISILPRVV+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEES+AHPTYKRKLVEMEATDYTNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ IRRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQH IQTH  S
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSS 331

[Close]

BLAST of MC00g0016 vs. ExPASy TrEMBL
Match: A0A1S3C1I8 (probable nitronate monooxygenase OS=Cucumis melo OX=3656 GN=LOC103495406 PE=4 SV=1)

HSP 1 Score: 620 bits (1598), Expect = 2.36e-223
Identity = 309/331 (93.35%), Postives = 320/331 (96.68%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD++RELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+KPFG+GVILAFPHEQNL+AILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVD GVDAIIVQGREAGGHVIGQEGLISILPRVV+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEES+AHPTYKRKLVEMEATDYTNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ IRRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQH IQTH  S
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSS 331

[Close]

BLAST of MC00g0016 vs. ExPASy TrEMBL
Match: A0A0A0K422 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G064010 PE=4 SV=1)

HSP 1 Score: 617 bits (1590), Expect = 2.88e-221
Identity = 307/331 (92.75%), Postives = 320/331 (96.68%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD++RELIRKTRA
Sbjct: 55  MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 114

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+KPFG+GVILAFPHEQNL+AILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 115 LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 174

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+IVG++DIPVIAAG ISDSRG
Sbjct: 175 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 234

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEESNAHPTYKRKLVE+EATDYTNIFGRARWP APQRVLQ
Sbjct: 235 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 294

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ IRRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 295 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 354

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVRRLVEGAQH I+TH  S
Sbjct: 355 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSS 385

[Close]

BLAST of MC00g0016 vs. ExPASy TrEMBL
Match: A0A6J1GL38 (uncharacterized protein LOC111454946 OS=Cucurbita moschata OX=3662 GN=LOC111454946 PE=4 SV=1)

HSP 1 Score: 610 bits (1574), Expect = 1.07e-219
Identity = 303/331 (91.54%), Postives = 318/331 (96.07%), Query Frame = 0


Query: 1   MAFGGILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRA 60
           M  GGILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLR+PDWESPD++RELIRKTRA
Sbjct: 1   MDSGGILGFRYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LT+KPFGVGVILAFPHEQNL+AILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGSV 120

Query: 121 EEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180
           EEA KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIP+IAAGGISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQ 240
           Y AALALGARGVCLGTRFVATEES+AHP YKRKLVE++ATDYTNIFGRARWP APQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRSTINGLD+ +RRFAGTVPNAT KGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 331
           VGLIKEILPAGEVVR+LVEGAQH IQTH  S
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSS 331

[Close]

BLAST of MC00g0016 vs. TAIR 10
Match: AT5G64250.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 525.4 bits (1352), Expect = 3.3e-149
Identity = 251/327 (76.76%), Postives = 290/327 (88.69%), Query Frame = 0


Query: 5   GILGFQYGIVQAPLGPDISSPELVAAVANAGGLGLLRAPDWESPDFIRELIRKTRALTEK 64
           GILGF+YGIVQAPLGPDIS PELVAAVANAGG+GLLR PDWE PD++RELIRKT+ LTEK
Sbjct: 3   GILGFEYGIVQAPLGPDISGPELVAAVANAGGIGLLRCPDWECPDYLRELIRKTKTLTEK 62

Query: 65  PFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAR 124
           PFG+GV+LAFPH+ N++ IL+EKVAV+Q+YWG+CS++LVD  H AGVK+IPQVG+VEEAR
Sbjct: 63  PFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDDAHFAGVKVIPQVGNVEEAR 122

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRGYAAA 184
           KAV VGVDAIIVQGREAGGHVIG++GL S+LPRVVD+VG  DIPVIAAGGI D RGY AA
Sbjct: 123 KAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGERDIPVIAAGGIVDGRGYVAA 182

Query: 185 LALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNIFGRARWPDAPQRVLQTPFY 244
           L+LGA+GVCLGTRFVAT ES AHP YKRKL+E E T+YT++FGRARWP APQRVL+TPF+
Sbjct: 183 LSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDVFGRARWPGAPQRVLETPFF 242

Query: 245 DDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNATAKGDIESMAMYAGQGVGLI 304
           DDW+SLPAHENE NQP+IGRSTI+G++  IRRF+GTVPN T  GD+ESMAMYAGQ VGLI
Sbjct: 243 DDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMTTTGDLESMAMYAGQSVGLI 302

Query: 305 KEILPAGEVVRRLVEGAQHQIQTHFRS 332
           KEILPAGEVV+ LVE AQ  I   F +
Sbjct: 303 KEILPAGEVVKSLVEEAQALILQKFNN 329

[Close]

BLAST of MC00g0016 vs. TAIR 10
Match: AT5G64250.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 448.0 bits (1151), Expect = 6.7e-126
Identity = 214/286 (74.83%), Postives = 251/286 (87.76%), Query Frame = 0


Query: 46  ESPDFIRELIRKTRALTEKPFGVGVILAFPHEQNLRAILDEKVAVVQVYWGECSKDLVDQ 105
           E PD++RELIRKT+ LTEKPFG+GV+LAFPH+ N++ IL+EKVAV+Q+YWG+CS++LVD 
Sbjct: 4   ECPDYLRELIRKTKTLTEKPFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDD 63

Query: 106 VHSAGVKIIPQVGSVEEARKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNE 165
            H AGVK+IPQVG+VEEARKAV VGVDAIIVQGREAGGHVIG++GL S+LPRVVD+VG  
Sbjct: 64  AHFAGVKVIPQVGNVEEARKAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGER 123

Query: 166 DIPVIAAGGISDSRGYAAALALGARGVCLGTRFVATEESNAHPTYKRKLVEMEATDYTNI 225
           DIPVIAAGGI D RGY AAL+LGA+GVCLGTRFVAT ES AHP YKRKL+E E T+YT++
Sbjct: 124 DIPVIAAGGIVDGRGYVAALSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDV 183

Query: 226 FGRARWPDAPQRVLQTPFYDDWKSLPAHENESNQPVIGRSTINGLDVGIRRFAGTVPNAT 285
           FGRARWP APQRVL+TPF+DDW+SLPAHENE NQP+IGRSTI+G++  IRRF+GTVPN T
Sbjct: 184 FGRARWPGAPQRVLETPFFDDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMT 243

Query: 286 AKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHQIQTHFRS 332
             GD+ESMAMYAGQ VGLIKEILPAGEVV+ LVE AQ  I   F +
Sbjct: 244 TTGDLESMAMYAGQSVGLIKEILPAGEVVKSLVEEAQALILQKFNN 289

The following BLAST results are available for this feature:

BLAST of MC00g0016 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0S7Q1	6.5e-33	39.33	(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA deh...	[more]
P71847	3.6e-31	37.25	(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA deh...	[more]
B2TEK6	2.6e-29	31.88	Nitronate monooxygenase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG...	[more]
Q9HWH9	2.2e-28	31.40	Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644...	[more]
Q01284	1.8e-27	28.85	Nitronate monooxygenase OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / C...	[more]

BLAST of MC00g0016 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022141996.1	1.90e-237	100.00	uncharacterized protein LOC111012234 isoform X1 [Momordica charantia]	[more]
XP_008455173.1	4.87e-223	93.35	PREDICTED: probable nitronate monooxygenase [Cucumis melo] >KAA0031461.1 putativ...	[more]
XP_031744639.1	8.07e-222	92.75	uncharacterized protein LOC101221123 [Cucumis sativus]	[more]
XP_038888874.1	3.28e-221	93.96	nitronate monooxygenase isoform X1 [Benincasa hispida]	[more]
KAG6588765.1	2.05e-219	91.24	hypothetical protein SDJN03_17330, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of MC00g0016 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CKV8	9.20e-238	100.00	uncharacterized protein LOC111012234 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A5A7SPI9	2.36e-223	93.35	Putative nitronate monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3C1I8	2.36e-223	93.35	probable nitronate monooxygenase OS=Cucumis melo OX=3656 GN=LOC103495406 PE=4 SV...	[more]
A0A0A0K422	2.88e-221	92.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G064010 PE=4 SV=1	[more]
A0A6J1GL38	1.07e-219	91.54	uncharacterized protein LOC111454946 OS=Cucurbita moschata OX=3662 GN=LOC1114549...	[more]

BLAST of MC00g0016 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64250.2	3.3e-149	76.76	Aldolase-type TIM barrel family protein	[more]
AT5G64250.1	6.7e-126	74.83	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (Dali-11) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004136	Nitronate monooxygenase	PFAM	PF03060	NMO	coord: 6..74 e-value: 3.1E-13 score: 49.8 coord: 76..319 e-value: 3.4E-47 score: 161.5
IPR004136	Nitronate monooxygenase	CDD	cd04730	NPD_like	coord: 10..234 e-value: 1.80527E-70 score: 217.351
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 3..331 e-value: 1.0E-99 score: 335.7
None	No IPR available	PANTHER	PTHR32332:SF35	SUBFAMILY NOT NAMED	coord: 3..326
None	No IPR available	PANTHER	PTHR32332	2-NITROPROPANE DIOXYGENASE	coord: 3..326
None	No IPR available	SUPERFAMILY	51412	Inosine monophosphate dehydrogenase (IMPDH)	coord: 3..325

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MC00g0016.1	MC00g0016.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0018580	nitronate monooxygenase activity
molecular_function	GO:0003824	catalytic activity