LsiUNG000320 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsiUNG000320
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionprotein TORNADO 2
Locationchr00: 518972 .. 520887 (+)
RNA-Seq ExpressionLsiUNG000320
SyntenyLsiUNG000320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACTAAACAAGACAGTAATGGGAGCCATAAACTTCATAGCCATGGTTCTATCAGTCCCCATCATCGGCGCCGGAATCTGGCTCGCCACCGAACAAGACAACGCCTGCGTCCAAATCCTCCAATGGCCTCTCATCATTTTCGGTGTCATCGTCCTTCTCGTTGCGGTCGCCGGTTTCATCGGAGCCTTTTGTCGCATCACTTGGCTACTCCTTCTTTACCTTGTAGCGATGCTCATTCTCATTATCCTCCTTGCTTGCTTGGTGGGCTTCATTTACGTGGTTACTATTAGAGGGAATGGCCATTTGGAGCCTAATAGAAGTTACTTGGAATATCATCTTGATGACTTTTCTGGGTTTCTTAGGCATAGAGTTCAAAGCTCTTTTAAATGGGATTTGATTCGAAGTTGTTTGAGTTCTTCTTCTATGTGTGCTGAATTGAATCAAAGCTTTCGTTTGGCTCAGGATTTCTTTACTGCTCCCATTACTCCTCTTCAGGTATGTATATATATGTAGATGAGTCTTTTTGTTTTTTTACCTACTTTTCTAACCATGATTTCAAAATATAAGCTAGGTTTAGAAAACTAAAAAATAAAAATAAAAATACTTTTTGAAAACCGTACAAAAGAATTTGAGACAAAAACAAAAATAAATTTCAAAAAACAGAAACTAAAAACAAAATGGTTATCAAACGAGGTCAAAGTATTTGAGAGAAAAAAAAAGACATAAATTATGTTTAAAAAAAATACTTTTGTGTCTCATTGTTACTTTTCATAGACGTAACAATTTAATTTTTATGAAATTGATAGCTAACAAATTATATAATCTCGGACGTGAAAAATATTAATAAAATCAAGTCATTACATAATAGAAAAAACAACACTTACTTTTTGACTGAGATTTTTCATAATAAAGATTAAATTAACAAGAACTGAAGTACTATACACTAAAGTTTATATACTAAAATTGTTACGAATGAACAAAAGTTTGCAAATTAAAACATAGAATATTCGTTCTAAAGATCAAAAATTGGAATCTCCACCGTACTTGTAGATACTTGTAAACGTAAACCAAATAAACCCTAATTTGGAAAAGTGAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTGACACAGTCAGGATGCTGCAAGCCACCAACATTATGTGGATACACATTTGTGAACCCAACATATTGGATAATGCCTATAAACAATGCTGCTGACATGGATTGTTTGAAGTGGAACAATGACCAAACTCAGCTCTGTTATGGTTGTGATTCTTGCAAGGCTGGTTTGCTTGAGAGCTTGAAGAATCAATGGAGGAAGGCTGATATTATACTCCTCTTGTCTCTTATTGCTCTCATTTCTATCTATATGATTGCTTGTTGTGTCTTTAGAAATGCCAAAACTGAGAAACTCTTTGACAAATACAAGCAAGGACAACCTCAACCATATGTCTAA

mRNA sequence

ATGGCACTAAACAAGACAGTAATGGGAGCCATAAACTTCATAGCCATGGTTCTATCAGTCCCCATCATCGGCGCCGGAATCTGGCTCGCCACCGAACAAGACAACGCCTGCGTCCAAATCCTCCAATGGCCTCTCATCATTTTCGGTGTCATCGTCCTTCTCGTTGCGGTCGCCGGTTTCATCGGAGCCTTTTGTCGCATCACTTGGCTACTCCTTCTTTACCTTGTAGCGATGCTCATTCTCATTATCCTCCTTGCTTGCTTGGTGGGCTTCATTTACGTGGTTACTATTAGAGGGAATGGCCATTTGGAGCCTAATAGAAGTTACTTGGAATATCATCTTGATGACTTTTCTGGGTTTCTTAGGCATAGAGTTCAAAGCTCTTTTAAATGGGATTTGATTCGAAGATGCTGCAAGCCACCAACATTATGTGGATACACATTTGTGAACCCAACATATTGGATAATGCCTATAAACAATGCTGCTGACATGGATTGTTTGAAGTGGAACAATGACCAAACTCAGCTCTGTTATGGTTGTGATTCTTGCAAGGCTGGTTTGCTTGAGAGCTTGAAGAATCAATGGAGGAAGGCTGATATTATACTCCTCTTGTCTCTTATTGCTCTCATTTCTATCTATATGATTGCTTGTTGTGTCTTTAGAAATGCCAAAACTGAGAAACTCTTTGACAAATACAAGCAAGGACAACCTCAACCATATGTCTAA

Coding sequence (CDS)

ATGGCACTAAACAAGACAGTAATGGGAGCCATAAACTTCATAGCCATGGTTCTATCAGTCCCCATCATCGGCGCCGGAATCTGGCTCGCCACCGAACAAGACAACGCCTGCGTCCAAATCCTCCAATGGCCTCTCATCATTTTCGGTGTCATCGTCCTTCTCGTTGCGGTCGCCGGTTTCATCGGAGCCTTTTGTCGCATCACTTGGCTACTCCTTCTTTACCTTGTAGCGATGCTCATTCTCATTATCCTCCTTGCTTGCTTGGTGGGCTTCATTTACGTGGTTACTATTAGAGGGAATGGCCATTTGGAGCCTAATAGAAGTTACTTGGAATATCATCTTGATGACTTTTCTGGGTTTCTTAGGCATAGAGTTCAAAGCTCTTTTAAATGGGATTTGATTCGAAGATGCTGCAAGCCACCAACATTATGTGGATACACATTTGTGAACCCAACATATTGGATAATGCCTATAAACAATGCTGCTGACATGGATTGTTTGAAGTGGAACAATGACCAAACTCAGCTCTGTTATGGTTGTGATTCTTGCAAGGCTGGTTTGCTTGAGAGCTTGAAGAATCAATGGAGGAAGGCTGATATTATACTCCTCTTGTCTCTTATTGCTCTCATTTCTATCTATATGATTGCTTGTTGTGTCTTTAGAAATGCCAAAACTGAGAAACTCTTTGACAAATACAAGCAAGGACAACCTCAACCATATGTCTAA

Protein sequence

MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGFIGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGFLRHRVQSSFKWDLIRRCCKPPTLCGYTFVNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIALISIYMIACCVFRNAKTEKLFDKYKQGQPQPYV
Homology
BLAST of LsiUNG000320 vs. ExPASy Swiss-Prot
Match: Q9FIQ5 (Protein TORNADO 2 OS=Arabidopsis thaliana OX=3702 GN=TRN2 PE=1 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 1.5e-87
Identity = 161/267 (60.30%), Postives = 196/267 (73.41%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           M L+  V+G INFI ++LS+P+IGAGIWLA    N+CV++LQWP+II GV++LLV +AGF
Sbjct: 1   MPLSNNVIGCINFITVLLSIPVIGAGIWLAIGTVNSCVKLLQWPVIILGVLILLVGLAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IG F RITWLL++YL+AMLILI+LL CLVGFIY+VTIRG+GH EP+R+YLEY L DFSG+
Sbjct: 61  IGGFWRITWLLVVYLIAMLILIVLLGCLVGFIYMVTIRGSGHPEPSRAYLEYSLQDFSGW 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LR RVQ S+KW+ IR                                 CCKPPT CG+TF
Sbjct: 121 LRRRVQRSYKWERIRTCLSTTTICPELNQRYTLAQDFFNAHLDPIQSGCCKPPTKCGFTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 236
           VNPTYWI PI+ +ADMDCL W+NDQ  LCY CDSCKAGLL ++K  W KADI LLL+LI 
Sbjct: 181 VNPTYWISPIDMSADMDCLNWSNDQNTLCYTCDSCKAGLLANIKVDWLKADIFLLLALIG 240

BLAST of LsiUNG000320 vs. ExPASy Swiss-Prot
Match: Q9ZUN5 (Tetraspanin-2 OS=Arabidopsis thaliana OX=3702 GN=TET2 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 2.7e-60
Identity = 120/268 (44.78%), Postives = 165/268 (61.57%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MAL   +   +N +A++ S+PI  +GIWLA++ DN CV +L+WP+++ GV++L+V+  GF
Sbjct: 1   MALANNLTAILNLLALLCSIPITASGIWLASKPDNECVNLLRWPVVVLGVLILVVSATGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGA+     LL +YL  M ILI LL  ++ F +VVT     +  P R Y EY L+ FS +
Sbjct: 61  IGAYKYKETLLAVYLCCMAILIGLLLVVLIFAFVVTRPDGSYRVPGRGYKEYRLEGFSNW 120

Query: 121 LRHRVQSSFKWDLIRR---------------------------------CCKPPTLCGYT 180
           L+  V  S  W  +R                                  CCKPPT CGY 
Sbjct: 121 LKENVVDSKNWGRLRACLADTNVCPKLNQEFITADQFFSSSKITPLQSGCCKPPTACGYN 180

Query: 181 FVNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLI 236
           FVNPT W+ P N AAD DC  W+NDQ+QLCY C+SCKAGLL +L+ +WRKA++IL+++++
Sbjct: 181 FVNPTLWLNPTNMAADADCYLWSNDQSQLCYNCNSCKAGLLGNLRKEWRKANLILIITVV 240

BLAST of LsiUNG000320 vs. ExPASy Swiss-Prot
Match: Q9M1E7 (Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.9e-45
Identity = 102/262 (38.93%), Postives = 151/262 (57.63%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNA-CVQILQWPLIIFGVIVLLVAVAG 60
           M  +  ++G +NF+  +LS+PI+G GIWL++  ++  C++ LQWPLI+ G+ +++V++AG
Sbjct: 1   MRTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSG 120
           F GA  R  +L+ LYLV ML++I  L   + F Y VT +G+G    NR YL+Y+L+D+SG
Sbjct: 61  FAGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSG 120

Query: 121 FLRHRVQSSFKWD-----------------------------LIRR-------CCKPPTL 180
           +L+ RV     W                               +RR       CCKPPT 
Sbjct: 121 WLKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRRLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINN-AADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIIL 225
           CG+++VN T W         + DC+ W+NDQ+ LCY C SCKAG+L SLK  WRK  +I 
Sbjct: 181 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

BLAST of LsiUNG000320 vs. ExPASy Swiss-Prot
Match: Q8S8Q6 (Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 4.1e-45
Identity = 93/263 (35.36%), Postives = 146/263 (55.51%), Query Frame = 0

Query: 4   NKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGFIGA 63
           +  ++G +NF+  +LS+PI+  GIWL+ +    C + L  P+I  GV +++VA+AG IG+
Sbjct: 5   SNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAGLIGS 64

Query: 64  FCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGFLRH 123
            CR+TWLL +YL  M +LI+L+ C+  F +VVT +G G     + Y EY L D+S +L+ 
Sbjct: 65  CCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYSTWLQK 124

Query: 124 RVQSSFKWDLIRR----------------------------------CCKPPTLCGYTFV 183
           RV++   W+ IR                                   CCKP   CG+ +V
Sbjct: 125 RVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECGFEYV 184

Query: 184 NPTYWIMPINNA-ADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 232
           NPT W         + DC  W+N + +LC+ C SCKAGLL+++K+ W+K  I+ ++ L+ 
Sbjct: 185 NPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIVFLVF 244

BLAST of LsiUNG000320 vs. ExPASy Swiss-Prot
Match: Q9LSS4 (Tetraspanin-4 OS=Arabidopsis thaliana OX=3702 GN=TET4 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.6e-44
Identity = 102/262 (38.93%), Postives = 145/262 (55.34%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNA-CVQILQWPLIIFGVIVLLVAVAG 60
           M     ++G INF   +LS+PI+G GIWL++  ++  C++ LQWPLII G+ ++++++AG
Sbjct: 1   MRSRSNLIGLINFFTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIIIGISIMVISLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSG 120
             GA  +  +L+ LYL  M  +I  L     F YVVT +G+G    NR YL+Y+L+D+SG
Sbjct: 61  IAGACYQNKFLMWLYLFTMFFVIAALIGFTIFAYVVTDKGSGRFVMNRRYLDYYLNDYSG 120

Query: 121 FLRHRVQSSFKWDLI------------------------------------RRCCKPPTL 180
           +L+ RV  +  W  I                                      CCKPPT 
Sbjct: 121 WLKDRVTDNGYWRDIGSCVRDSGVCKKIGRDLNGVPETAHMFYFRNLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINNAA-DMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIIL 225
           CGYT+VN T WI        + DC+ WNNDQ  LCY C SCKAG+L SLK  WRK  +I 
Sbjct: 181 CGYTYVNETVWIPGGEMVGPNPDCMLWNNDQRLLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

BLAST of LsiUNG000320 vs. ExPASy TrEMBL
Match: A0A0A0LEA8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G901690 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 8.2e-121
Identity = 225/274 (82.12%), Postives = 236/274 (86.13%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MALNKTVMGAINFIAMV+S+PIIGAGIWLAT+QDNACVQILQWPLIIFGVIVLLVAVAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVIVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAFCRI WLL+ YLVAMLILI+LL CLVGFIY+VTIRG+GHLEPNRSYLEYHL+DFSGF
Sbjct: 61  IGAFCRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRVQSSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQGQ-PQPYV 242
           LIS+YMIA CVFRNAKT+KLFDKYKQGQ PQPY+
Sbjct: 241 LISVYMIAACVFRNAKTQKLFDKYKQGQPPQPYI 274

BLAST of LsiUNG000320 vs. ExPASy TrEMBL
Match: A0A5A7T5J8 (Protein TORNADO 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004550 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.5e-119
Identity = 221/274 (80.66%), Postives = 235/274 (85.77%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MALNKTVMGAINFIAMV+S+PIIGAGIWLAT+QDNACVQILQWPLIIFGV+VLLVAVAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVVVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI WLL+ YLVAMLILI+LL CLVGFIY+VTIRG+GHLEPNRSYLEYHL+DFSGF
Sbjct: 61  IGAFLRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRVQSSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQGQ-PQPYV 242
           L+S+Y+IA CVFRNAKT+KLFDKYKQGQ PQPY+
Sbjct: 241 LVSVYLIAACVFRNAKTQKLFDKYKQGQPPQPYI 274

BLAST of LsiUNG000320 vs. ExPASy TrEMBL
Match: A0A1S3CQH2 (protein TORNADO 2 OS=Cucumis melo OX=3656 GN=LOC103503551 PE=4 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 4.5e-119
Identity = 221/274 (80.66%), Postives = 235/274 (85.77%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MALNKTVMGAINFIAMV+S+PIIGAGIWLAT+QDNACVQILQWPLIIFGV+VLLVAVAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVVVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI WLL+ YLVAMLILI+LL CLVGFIY+VTIRG+GHLEPNRSYLEYHL+DFSGF
Sbjct: 61  IGAFLRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRVQSSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQGQ-PQPYV 242
           L+S+Y+IA CVFRNAKT+KLFDKYKQGQ PQPY+
Sbjct: 241 LVSVYLIAACVFRNAKTQKLFDKYKQGQPPQPYI 274

BLAST of LsiUNG000320 vs. ExPASy TrEMBL
Match: A0A6J1KA46 (protein TORNADO 2 OS=Cucurbita maxima OX=3661 GN=LOC111491491 PE=4 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 3.0e-115
Identity = 212/274 (77.37%), Postives = 234/274 (85.40%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MAL KTV+GAINFIAM+LSVPIIGAGIWLAT+QDNACVQILQWP+I FGVIVL+VAVAGF
Sbjct: 1   MALTKTVIGAINFIAMLLSVPIIGAGIWLATQQDNACVQILQWPIIFFGVIVLIVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI+WLL++YL+AML+LI+LLACLVGFIY+VTIRG+GHLEPNR+YLEYHLDDFSGF
Sbjct: 61  IGAFWRISWLLIVYLIAMLVLIVLLACLVGFIYMVTIRGSGHLEPNRAYLEYHLDDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRV+SSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVESSFKWDLIRSCLSSSSMCGQLNQSFRMAQDFFNAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADI+LLL+L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIVLLLALVA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQG-QPQPYV 242
           LIS+Y+IACC F+NAKTEKLFDKYKQG + Q YV
Sbjct: 241 LISVYLIACCAFKNAKTEKLFDKYKQGRERQGYV 274

BLAST of LsiUNG000320 vs. ExPASy TrEMBL
Match: A0A6J1EYR1 (protein TORNADO 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437433 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.1e-114
Identity = 212/274 (77.37%), Postives = 233/274 (85.04%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MAL KTV+GAINFIAM+LSVPIIGAGIWLAT+QDNACVQILQWP+I FGVIVL+VAVAGF
Sbjct: 1   MALIKTVIGAINFIAMLLSVPIIGAGIWLATQQDNACVQILQWPIIFFGVIVLIVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI+WLL++YL+AML+LI+LLACLVGFIY+VTIRG+GHLEPNR+YLEYHLDDFSGF
Sbjct: 61  IGAFWRISWLLIVYLIAMLVLIVLLACLVGFIYMVTIRGSGHLEPNRAYLEYHLDDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRV+SSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVESSFKWDLIRSCLSSSSTCGQLNQSFRMAQDFFNAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADI+LLL L+A
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIVLLLVLVA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQG-QPQPYV 242
           LIS+Y+IACC F+NAKTEKLFDKYKQG + Q YV
Sbjct: 241 LISVYLIACCAFKNAKTEKLFDKYKQGRESQGYV 274

BLAST of LsiUNG000320 vs. NCBI nr
Match: XP_038888578.1 (protein TORNADO 2-like [Benincasa hispida])

HSP 1 Score: 448.4 bits (1152), Expect = 4.0e-122
Identity = 226/273 (82.78%), Postives = 236/273 (86.45%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MALNKTVMGAINFIAMV+S+PIIGAGIWLAT+QDNACVQILQWP+IIFGV+VLLVA+AGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPIIIFGVVVLLVAIAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAFCRITWLLL+YLVAMLILI+LL CLVGFIY+VTIRG+GHLEPNRSYLEYHLDDFSGF
Sbjct: 61  IGAFCRITWLLLVYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLDDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRVQSSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRMAQDFFTAPITPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINN ADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA
Sbjct: 181 VNPTYWIMPINNGADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQGQPQPYV 242
           LISIYMIA CVFRNAKTEKLFDKYKQ QPQPYV
Sbjct: 241 LISIYMIAFCVFRNAKTEKLFDKYKQAQPQPYV 273

BLAST of LsiUNG000320 vs. NCBI nr
Match: XP_004136394.1 (protein TORNADO 2 [Cucumis sativus] >KGN60375.1 hypothetical protein Csa_001399 [Cucumis sativus])

HSP 1 Score: 443.0 bits (1138), Expect = 1.7e-120
Identity = 225/274 (82.12%), Postives = 236/274 (86.13%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MALNKTVMGAINFIAMV+S+PIIGAGIWLAT+QDNACVQILQWPLIIFGVIVLLVAVAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVIVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAFCRI WLL+ YLVAMLILI+LL CLVGFIY+VTIRG+GHLEPNRSYLEYHL+DFSGF
Sbjct: 61  IGAFCRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRVQSSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQGQ-PQPYV 242
           LIS+YMIA CVFRNAKT+KLFDKYKQGQ PQPY+
Sbjct: 241 LISVYMIAACVFRNAKTQKLFDKYKQGQPPQPYI 274

BLAST of LsiUNG000320 vs. NCBI nr
Match: XP_008465983.1 (PREDICTED: protein TORNADO 2 [Cucumis melo] >KAA0038570.1 protein TORNADO 2 [Cucumis melo var. makuwa] >TYK31173.1 protein TORNADO 2 [Cucumis melo var. makuwa])

HSP 1 Score: 437.2 bits (1123), Expect = 9.3e-119
Identity = 221/274 (80.66%), Postives = 235/274 (85.77%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MALNKTVMGAINFIAMV+S+PIIGAGIWLAT+QDNACVQILQWPLIIFGV+VLLVAVAGF
Sbjct: 1   MALNKTVMGAINFIAMVVSIPIIGAGIWLATQQDNACVQILQWPLIIFGVVVLLVAVAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI WLL+ YLVAMLILI+LL CLVGFIY+VTIRG+GHLEPNRSYLEYHL+DFSGF
Sbjct: 61  IGAFLRINWLLIAYLVAMLILIVLLGCLVGFIYMVTIRGSGHLEPNRSYLEYHLEDFSGF 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRVQSSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 121 LRHRVQSSFKWDLIRSCLSSSSMCAELNQSFRLAQDFFTAPISPLQSGCCKPPTLCGYTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA
Sbjct: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQGQ-PQPYV 242
           L+S+Y+IA CVFRNAKT+KLFDKYKQGQ PQPY+
Sbjct: 241 LVSVYLIAACVFRNAKTQKLFDKYKQGQPPQPYI 274

BLAST of LsiUNG000320 vs. NCBI nr
Match: KAG7036197.1 (Protein TORNADO 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 424.9 bits (1091), Expect = 4.8e-115
Identity = 212/274 (77.37%), Postives = 234/274 (85.40%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MAL KTV+GAINFIAM+LSVPIIGAGIWLAT+QDNACVQILQWP+I FGVIVL+VAVAGF
Sbjct: 67  MALTKTVIGAINFIAMLLSVPIIGAGIWLATQQDNACVQILQWPIIFFGVIVLIVAVAGF 126

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI+WLL++YL+AML+LI+LLACLVGFIY+VTIRG+GHLEPNR+YLEYHLDDFSGF
Sbjct: 127 IGAFWRISWLLIVYLIAMLVLIVLLACLVGFIYMVTIRGSGHLEPNRAYLEYHLDDFSGF 186

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRV+SSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 187 LRHRVESSFKWDLIRSCLSSSSTCGQLNQSFRMAQDFFNAPISPLQSGCCKPPTLCGYTF 246

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADI+LLL+L+A
Sbjct: 247 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIVLLLALVA 306

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQG-QPQPYV 242
           LIS+Y+IACC F+NAKTEKLFDKYKQG + Q YV
Sbjct: 307 LISVYLIACCAFKNAKTEKLFDKYKQGRESQGYV 340

BLAST of LsiUNG000320 vs. NCBI nr
Match: XP_023533678.1 (protein TORNADO 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.9 bits (1091), Expect = 4.8e-115
Identity = 212/274 (77.37%), Postives = 234/274 (85.40%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MAL KTV+GAINFIAM+LSVPIIGAGIWLAT+QDNACVQILQWP+I FGVIVL+VAVAGF
Sbjct: 58  MALTKTVIGAINFIAMLLSVPIIGAGIWLATQQDNACVQILQWPIIFFGVIVLIVAVAGF 117

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGAF RI+WLL++YL+AML+LI+LLACLVGFIY+VTIRG+GHLEPNR+YLEYHLDDFSGF
Sbjct: 118 IGAFWRISWLLIVYLIAMLVLIVLLACLVGFIYMVTIRGSGHLEPNRAYLEYHLDDFSGF 177

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LRHRV+SSFKWDLIR                                 CCKPPTLCGYTF
Sbjct: 178 LRHRVESSFKWDLIRSCLSSSSTCGQLNQSFRMAQDFFNAPISPLQSGCCKPPTLCGYTF 237

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 240
           VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADI+LLL+L+A
Sbjct: 238 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIVLLLALVA 297

Query: 241 LISIYMIACCVFRNAKTEKLFDKYKQG-QPQPYV 242
           LIS+Y+IACC F+NAKTEKLFDKYKQG + Q YV
Sbjct: 298 LISVYLIACCAFKNAKTEKLFDKYKQGRESQGYV 331

BLAST of LsiUNG000320 vs. TAIR 10
Match: AT5G46700.1 (Tetraspanin family protein )

HSP 1 Score: 323.9 bits (829), Expect = 1.1e-88
Identity = 161/267 (60.30%), Postives = 196/267 (73.41%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           M L+  V+G INFI ++LS+P+IGAGIWLA    N+CV++LQWP+II GV++LLV +AGF
Sbjct: 1   MPLSNNVIGCINFITVLLSIPVIGAGIWLAIGTVNSCVKLLQWPVIILGVLILLVGLAGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IG F RITWLL++YL+AMLILI+LL CLVGFIY+VTIRG+GH EP+R+YLEY L DFSG+
Sbjct: 61  IGGFWRITWLLVVYLIAMLILIVLLGCLVGFIYMVTIRGSGHPEPSRAYLEYSLQDFSGW 120

Query: 121 LRHRVQSSFKWDLIRR--------------------------------CCKPPTLCGYTF 180
           LR RVQ S+KW+ IR                                 CCKPPT CG+TF
Sbjct: 121 LRRRVQRSYKWERIRTCLSTTTICPELNQRYTLAQDFFNAHLDPIQSGCCKPPTKCGFTF 180

Query: 181 VNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 236
           VNPTYWI PI+ +ADMDCL W+NDQ  LCY CDSCKAGLL ++K  W KADI LLL+LI 
Sbjct: 181 VNPTYWISPIDMSADMDCLNWSNDQNTLCYTCDSCKAGLLANIKVDWLKADIFLLLALIG 240

BLAST of LsiUNG000320 vs. TAIR 10
Match: AT2G19580.1 (tetraspanin2 )

HSP 1 Score: 233.4 bits (594), Expect = 1.9e-61
Identity = 120/268 (44.78%), Postives = 165/268 (61.57%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGF 60
           MAL   +   +N +A++ S+PI  +GIWLA++ DN CV +L+WP+++ GV++L+V+  GF
Sbjct: 1   MALANNLTAILNLLALLCSIPITASGIWLASKPDNECVNLLRWPVVVLGVLILVVSATGF 60

Query: 61  IGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGF 120
           IGA+     LL +YL  M ILI LL  ++ F +VVT     +  P R Y EY L+ FS +
Sbjct: 61  IGAYKYKETLLAVYLCCMAILIGLLLVVLIFAFVVTRPDGSYRVPGRGYKEYRLEGFSNW 120

Query: 121 LRHRVQSSFKWDLIRR---------------------------------CCKPPTLCGYT 180
           L+  V  S  W  +R                                  CCKPPT CGY 
Sbjct: 121 LKENVVDSKNWGRLRACLADTNVCPKLNQEFITADQFFSSSKITPLQSGCCKPPTACGYN 180

Query: 181 FVNPTYWIMPINNAADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLI 236
           FVNPT W+ P N AAD DC  W+NDQ+QLCY C+SCKAGLL +L+ +WRKA++IL+++++
Sbjct: 181 FVNPTLWLNPTNMAADADCYLWSNDQSQLCYNCNSCKAGLLGNLRKEWRKANLILIITVV 240

BLAST of LsiUNG000320 vs. TAIR 10
Match: AT3G45600.1 (tetraspanin3 )

HSP 1 Score: 184.1 bits (466), Expect = 1.3e-46
Identity = 102/262 (38.93%), Postives = 151/262 (57.63%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNA-CVQILQWPLIIFGVIVLLVAVAG 60
           M  +  ++G +NF+  +LS+PI+G GIWL++  ++  C++ LQWPLI+ G+ +++V++AG
Sbjct: 1   MRTSNHLIGLVNFLTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIVIGISIMVVSLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSG 120
           F GA  R  +L+ LYLV ML++I  L   + F Y VT +G+G    NR YL+Y+L+D+SG
Sbjct: 61  FAGACYRNKFLMWLYLVVMLLIIAALIGFIIFAYAVTDKGSGRTVLNRGYLDYYLEDYSG 120

Query: 121 FLRHRVQSSFKWD-----------------------------LIRR-------CCKPPTL 180
           +L+ RV     W                               +RR       CCKPPT 
Sbjct: 121 WLKDRVSDDSYWGKISSCLRDSGACRKIGRNFNGVPETADMFFLRRLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINN-AADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIIL 225
           CG+++VN T W         + DC+ W+NDQ+ LCY C SCKAG+L SLK  WRK  +I 
Sbjct: 181 CGFSYVNETGWDTRGGMIGPNQDCMVWSNDQSMLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

BLAST of LsiUNG000320 vs. TAIR 10
Match: AT2G23810.1 (tetraspanin8 )

HSP 1 Score: 183.0 bits (463), Expect = 2.9e-46
Identity = 93/263 (35.36%), Postives = 146/263 (55.51%), Query Frame = 0

Query: 4   NKTVMGAINFIAMVLSVPIIGAGIWLATEQDNACVQILQWPLIIFGVIVLLVAVAGFIGA 63
           +  ++G +NF+  +LS+PI+  GIWL+ +    C + L  P+I  GV +++VA+AG IG+
Sbjct: 5   SNNLVGILNFLVFLLSIPILAGGIWLSQKGSTECERFLDKPVIALGVFLMVVAIAGLIGS 64

Query: 64  FCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSGFLRH 123
            CR+TWLL +YL  M +LI+L+ C+  F +VVT +G G     + Y EY L D+S +L+ 
Sbjct: 65  CCRVTWLLWVYLFVMFLLILLVFCITVFAFVVTNKGAGEAIEGKGYKEYKLGDYSTWLQK 124

Query: 124 RVQSSFKWDLIRR----------------------------------CCKPPTLCGYTFV 183
           RV++   W+ IR                                   CCKP   CG+ +V
Sbjct: 125 RVENGKNWNKIRSCLVESKVCSKLEAKFVNVPVNSFYKEHLTALQSGCCKPSDECGFEYV 184

Query: 184 NPTYWIMPINNA-ADMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIILLLSLIA 232
           NPT W         + DC  W+N + +LC+ C SCKAGLL+++K+ W+K  I+ ++ L+ 
Sbjct: 185 NPTTWTKNTTGTHTNPDCQTWDNAKEKLCFDCQSCKAGLLDNVKSAWKKVAIVNIVFLVF 244

BLAST of LsiUNG000320 vs. TAIR 10
Match: AT5G60220.1 (tetraspanin4 )

HSP 1 Score: 181.0 bits (458), Expect = 1.1e-45
Identity = 102/262 (38.93%), Postives = 145/262 (55.34%), Query Frame = 0

Query: 1   MALNKTVMGAINFIAMVLSVPIIGAGIWLATEQDNA-CVQILQWPLIIFGVIVLLVAVAG 60
           M     ++G INF   +LS+PI+G GIWL++  ++  C++ LQWPLII G+ ++++++AG
Sbjct: 1   MRSRSNLIGLINFFTFLLSIPILGGGIWLSSRANSTDCLRFLQWPLIIIGISIMVISLAG 60

Query: 61  FIGAFCRITWLLLLYLVAMLILIILLACLVGFIYVVTIRGNGHLEPNRSYLEYHLDDFSG 120
             GA  +  +L+ LYL  M  +I  L     F YVVT +G+G    NR YL+Y+L+D+SG
Sbjct: 61  IAGACYQNKFLMWLYLFTMFFVIAALIGFTIFAYVVTDKGSGRFVMNRRYLDYYLNDYSG 120

Query: 121 FLRHRVQSSFKWDLI------------------------------------RRCCKPPTL 180
           +L+ RV  +  W  I                                      CCKPPT 
Sbjct: 121 WLKDRVTDNGYWRDIGSCVRDSGVCKKIGRDLNGVPETAHMFYFRNLSPVESGCCKPPTD 180

Query: 181 CGYTFVNPTYWIMPINNAA-DMDCLKWNNDQTQLCYGCDSCKAGLLESLKNQWRKADIIL 225
           CGYT+VN T WI        + DC+ WNNDQ  LCY C SCKAG+L SLK  WRK  +I 
Sbjct: 181 CGYTYVNETVWIPGGEMVGPNPDCMLWNNDQRLLCYQCSSCKAGVLGSLKKSWRKVSVIN 240

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIQ51.5e-8760.30Protein TORNADO 2 OS=Arabidopsis thaliana OX=3702 GN=TRN2 PE=1 SV=1[more]
Q9ZUN52.7e-6044.78Tetraspanin-2 OS=Arabidopsis thaliana OX=3702 GN=TET2 PE=2 SV=1[more]
Q9M1E71.9e-4538.93Tetraspanin-3 OS=Arabidopsis thaliana OX=3702 GN=TET3 PE=1 SV=1[more]
Q8S8Q64.1e-4535.36Tetraspanin-8 OS=Arabidopsis thaliana OX=3702 GN=TET8 PE=2 SV=1[more]
Q9LSS41.6e-4438.93Tetraspanin-4 OS=Arabidopsis thaliana OX=3702 GN=TET4 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LEA88.2e-12182.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G901690 PE=4 SV=1[more]
A0A5A7T5J84.5e-11980.66Protein TORNADO 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00... [more]
A0A1S3CQH24.5e-11980.66protein TORNADO 2 OS=Cucumis melo OX=3656 GN=LOC103503551 PE=4 SV=1[more]
A0A6J1KA463.0e-11577.37protein TORNADO 2 OS=Cucurbita maxima OX=3661 GN=LOC111491491 PE=4 SV=1[more]
A0A6J1EYR11.1e-11477.37protein TORNADO 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437433 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888578.14.0e-12282.78protein TORNADO 2-like [Benincasa hispida][more]
XP_004136394.11.7e-12082.12protein TORNADO 2 [Cucumis sativus] >KGN60375.1 hypothetical protein Csa_001399 ... [more]
XP_008465983.19.3e-11980.66PREDICTED: protein TORNADO 2 [Cucumis melo] >KAA0038570.1 protein TORNADO 2 [Cuc... [more]
KAG7036197.14.8e-11577.37Protein TORNADO 2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023533678.14.8e-11577.37protein TORNADO 2-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G46700.11.1e-8860.30Tetraspanin family protein [more]
AT2G19580.11.9e-6144.78tetraspanin2 [more]
AT3G45600.11.3e-4638.93tetraspanin3 [more]
AT2G23810.12.9e-4635.36tetraspanin8 [more]
AT5G60220.11.1e-4538.93tetraspanin4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 5..218
e-value: 4.0E-25
score: 88.9
IPR044991Tetraspani, plantPANTHERPTHR32191TETRASPANIN-8-RELATEDcoord: 1..137
IPR044991Tetraspani, plantPANTHERPTHR32191TETRASPANIN-8-RELATEDcoord: 137..235
NoneNo IPR availablePANTHERPTHR32191:SF14PROTEIN TORNADO 2coord: 137..235
NoneNo IPR availablePANTHERPTHR32191:SF14PROTEIN TORNADO 2coord: 1..137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
LsiUNG000320.1LsiUNG000320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma