Lsi11G017000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G017000
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionmitotic checkpoint serine/threonine-protein kinase BUB1
Locationchr11: 25171808 .. 25176496 (-)
RNA-Seq ExpressionLsi11G017000
SyntenyLsi11G017000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCTCTTCTTGGAGCCAATGTACAGTCCGAGTCGGCATCTTCCACTTCTCTTCCTTAAACGAGCAGAAAGTTACTAGCAATGGCGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAGGACCCTTTGTTGCCTTGGCTCTGGTATTCCTCGACTACTTTTATGCGATCGAGCTTATTGACAGCCGTTCTTACATTCAATCTCAGGAAAATTGTTGGTGCTATTTGATTCCAATTGCAGGTCCATCAAAGATGCTCTTGAAAATTCAACTTCAGGCAGTGTTTCTGGGCCAGAACTCTCCAAGCTTCTCTCTGATTGCATTGAAAATTTCAGCGGCAATGTTATGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTGTGCGTAACTATCTTTCTTTCACTGTTACATTTGTGGGTTTAACTTATCATCTTTGTTTCATACATGCGGTTGATTAGATAACTTGTATGTGACTATGTGTCATGCTTTTGATGAAGTATCTTTTGAAGGTTTCTTATGCCTACCTATTTCGCTGCCCTTCTCTTTCCTTTTCTAATCATGCTCACTTTAGGTTATTGCGGGTTTATCTGGGTTCATAATAAAATCGATTCTTCCTTTGGTTATTATACTTGAATTTTTTTGTATTATGTGTAAATGCTTGGATCTCTTCTAAAACTTGGGCATTTCCGACAAGTTTTTAATTCGTTTATGGATTTTGAATAATAATTAACATTATGTTCGTGTAAGTAGATTGGTATGAGGGAGGATTTTGAAACTGGTTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTCCTCGAATCCAAGGGGAGGTTAAATGACGCTAATATAGTTTATGAGCTTGGGTTGATGAGGTATTCCTATCTCTCTCTCATTGACATGCTGTTAAATACATGTAGATTATGCTTGTGTGAAAACTTTTGTTGATTAGGATTTTTTGTTTGCTAGTGTTCTGTGTGTTTTGTTCCTTACATTTTTAAGTGATAAATTGATAATAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAACATTGTTTATTGATAGGATATCTGAGTTGGTCAATACTCATTCAATGCAGGGGGTACGTGATCTTAAAGCAAAAAAACCATCAAGGTTCATGTTCCTTAAATTGTTTAATTTAAAGAATTTTGACACGGTTGGTTTCATTTTTGTAGAATGATGGTAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAGCAAGATTAATCCAGAGATCATGAAGTATGATGTAAGAAAATGCAGTCTCTTTTGCCAGATGATGAAAGCAAGCTGGTTATATTGTTCAAATAAATTACTCCCAAACTAACTTTACTAAGAATTTAATGCGTGCTTCAGGGTTACCATTCCAGTACCAAAGTTTACTCTAAAAAGATGGCTCTAAAGTCATTGCGCAATTCATCAAGAAATAAGATTATTGAGATTGGTAATTTAATGTTATTCATTATAGTTAGAATTGTCATCTAAATGTATAAAACTACCTTCAGTCCATCCTTCAAAAGAACGAGCTTTCTTGAAATATTGTGTAAGAAATGAAGGAAAAAGATTTCTTATGTCACAGATTCGTTTTTCATTATAATAATACACTTGCCAACATTTACAGGTGGAAAGAAGTATGAGATCAAAGGCTGTGCAGGTACTGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGACCATGTAGTAGCACTGAAGGTAAAAACTCTTATTGATTAACATTCAATCTGCATATGTCCTTTACTTCCAGCATTCCTCATAAAGTTATACGTCATACCTGAATGTGAACTAGTTGGATATCGTGATTCCTTTCAAATGCTTGTCAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTCGATCTTCGTGTCACCGGGAAGGAAGTATGTAGTAGCCCTTATTTTCATTAAGCATTTATTACTCTGAATGTTAGCAACTCGATTTATACTCATCCTCGATCTCTCATAATGTCAGAGGTCAAGTTTTGGTTGTGCTCATGACATGTATCTCTATTCTGACTACAGCATCCTTATATGTGATTACTTGGCTCATGGAACATTACAGGTGACTTTTCAGATTCTGAACCCTTCTATACTGTATGATCCTTTAAAATATTCATTGTAAGTAACCGTCTAGTTAAAACCTTGTGCAGGATGCCATAAATTTGTATGTAGTTCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTTGAAACTTTGCACAGCATTGGCATTATCCATGGAGATTTTAAACCTGACAATCTGCTTATTCGTTATGCGGGGTAAAAACATTTTATTGTTCTAAATTTTGATGGATGACATCCATCTTTTTGAGAGTTGATTTCATTATATAGTTCCCAATTACAATACTCGTGAAAAAAAGAAAAGAACTGTAACAAATGAAAAGAAAAGACGTCTAGTTGTTATTTATAGGGAAAAGTACCATTTTGGTCCTTAAGTTTTGAGAAATAGGTGTGTTTAGTCCCTCATGTTTTAAATTGGCCAATAGAGTCCTCAAGTTTTCAATAATGGGTTTAAATGGTACCTCTATTAAAAAAATTCATTAAAATTGAATAACAAATTGACTAAATACTGAAGTGGCGGGATGAGAGACTAAATGCACCTATTCCTCAAAACTCAGGGACTAAAAAGGTATTTTTTCCTTATTTATAATTTCAGCCTACCAATAATGATTAGCACCCGACTGGAGAATGTACTCCAAATCAATAGTTCTGTACCAATTGATTTTATTTAACCGTTATGCAGAGACAACCTAATAGAAGATGAATTTCAGGAACGTAGTGGTCCTTGGCAAGATCAGGTATTTAGTATCATTCCCTTTTTTGTTCCCAAGTCATTGCATGTCTATATGGAATGACTTTTTAAGTGTTGAAAAAAAGCGTTTTTGAGTGAAAAAATGTGCTTAAAGCACTTTAAAAGCCATTTCAAACCAACCCTTCATTAACTGTGAGCAAACAAGGTTATTTGTAGCTTTATATTGTAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATTTGCACCTCTTTCCTGAAAATGTAGAATTTAAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTCCAGAAATGCTAGAGAATAAACCCTGGAAATATCAGGTCAGTTTCTTCTGCAATGTGGTTATTTATAAAAACTCTAGAGAAACCCTCTGCAAAGAAGAAAACAAAAGCTCTACCAATAGCATTCTGTCTTCTGTACAGGTAGACATATATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAAACATCATCTGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTAGTTAACTATTCTGACACCTTTATTTGTCTCTTTTCTGGTTTTTTTCCCCATAAATATGTTCTTGAAATGCCTCGAATCCATTGTCTAGCGTTCCGAATGACTAAACCAGAGATCTCGCTGCCCAGTCAGTGAGTCAGAGTCTAGGGTCAGTTATTTACAAATTTGACCCTAGGGTTTAGAGAAGGAATGCGCTATATAAACTCTTTAACTCTACTGGCGCTTATGAATTTGTAATTTGATATTTTCTCTCTAATAAAAAATTGCTCTCTCCCTTGGCCCATGAACATAGCTTACACTGTTAGTGAACCACATACATCTGTGTCGATTCTCTATTGTTTACGTTGTTTATTTTCTTCATTTGTCAATTCCATAACATATGTTGTGCATAAATTGTAACTTTGGTAGATTCTTTGTAGAGTTTCAGATCAGCGATTTAGCTTTATTAGAGAGCATCCATCCTCTTCACCTCCTCCCGTCTGATTTGATTTCTCTGTTCCTCCATATAGGTATTGGAACATTGAACTGTGGAAGAATCTCTTCACAAAGTTGCTTAATATCACACCAGGTGATTGCAACAAAGAGTTGTTGCAGAGTATAAGGAAATCATTCCAAGAATACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTTGTAAAGCAAAGGGCTTCCTTGTGCACTGCCTAATCAGGAAACCCCCTTGGAAGGTGTAAATTCCCCCAAAAATGAAGGTGGTAGAAGTCAACATTTGTATGGCATGGTTCATGTTTGCCGAAGTCAACATTTGTATGGCATGGTTCATGTTTGCCACTTGCAAACTTGGATTAGTTCAGTTGATTTGTGATGAAATGTTTGTATTCTTTTTTCTCGGATGTATTAAAACCAAAATAGATTGAAGAGTTGTAAGCTAAGGGGTCTTCTTTGAAACGGTGTGTCTTGTAGGCTTCATTGTTTGTCTTAGAGGTTCTACTGTAATGTAGTAGGTTGTAGATATTCATTTATTTCACTTTGTTTTCTTTAAAATTTTATTAGATCTGATTTCTCTTGGAAGTCAAAAAGAATAGAGAATCATTTCACATAGGCTGCATTGTTCACTTCAATCAAAATATAACTCACTTCAT

mRNA sequence

CGCTCTTCTTGGAGCCAATGTACAGTCCGAGTCGGCATCTTCCACTTCTCTTCCTTAAACGAGCAGAAAGTTACTAGCAATGGCGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAGGACCCTTTGTTGCCTTGGCTCTGGTATTCCTCGACTACTTTTATGCGATCGAGCTTATTGACAGCCGTTCTTACATTCAATCTCAGGAAAATTGTTGGTGCTATTTGATTCCAATTGCAGGCAGTGTTTCTGGGCCAGAACTCTCCAAGCTTCTCTCTGATTGCATTGAAAATTTCAGCGGCAATGTTATGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGTTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTCCTCGAATCCAAGGGGAGGTTAAATGACGCTAATATAGTTTATGAGCTTGGGTTGATGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAACATTGTTTATTGATAGGATATCTGAGTTGGTCAATACTCATTCAATGCAGGGGAATGATGGTAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAGCAAGATTAATCCAGAGATCATGAAGTATGATTTAGAATTGTCATCTAAATGTATAAAACTACCTTCAGTCCATCCTTCAAAAGAACGAGCTTTCTTGAAATATTGTGTAAGAAATGAAGGAAAAAGATTTCTTATGTCACAGATTCGTGGAAAGAAGTATGAGATCAAAGGCTGTGCAGGTACTGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGACCATGTAGTAGCACTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTCGATCTTCGTGTCACCGGGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGACATGTATCTCTATTCTGACTACAGCATCCTTATATGTGATTACTTGGCTCATGGAACATTACAGGATGCCATAAATTTGTATGTAGTTCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTTGAAACTTTGCACAGCATTGGCATTATCCATGGAGATTTTAAACCTGACAATCTGCTTATTCGTTATGCGGGAGACAACCTAATAGAAGATGAATTTCAGGAACGTAGTGAATTTAAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTCCAGAAATGCTAGAGAATAAACCCTGGAAATATCAGGTCAGTTTCTTCTGCAATGTGGTTATTTATAAAAACTCTAGAGAAACCCTCTGCAAAGAAGAAAACAAAAGCTCTACCAATAGCATTCTGTCTTCTGTACAGGTAGACATATATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAAACATCATCTGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAACTGTGGAAGAATCTCTTCACAAAGTTGCTTAATATCACACCAGGTGATTGCAACAAAGAGTTGTTGCAGAGTATAAGGAAATCATTCCAAGAATACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTTGTAAAGCAAAGGGCTTCCTTGTGCACTGCCTAATCAGGAAACCCCCTTGGAAGGTGTAAATTCCCCCAAAAATGAAGGTGGTAGAAGTCAACATTTGTATGGCATGGTTCATGTTTGCCGAAGTCAACATTTGTATGGCATGGTTCATGTTTGCCACTTGCAAACTTGGATTAGTTCAGTTGATTTGTGATGAAATGTTTGTATTCTTTTTTCTCGGATGTATTAAAACCAAAATAGATTGAAGAGTTGTAAGCTAAGGGGTCTTCTTTGAAACGGTGTGTCTTGTAGGCTTCATTGTTTGTCTTAGAGGTTCTACTGTAATGTAGTAGGTTGTAGATATTCATTTATTTCACTTTGTTTTCTTTAAAATTTTATTAGATCTGATTTCTCTTGGAAGTCAAAAAGAATAGAGAATCATTTCACATAGGCTGCATTGTTCACTTCAATCAAAATATAACTCACTTCAT

Coding sequence (CDS)

CGCTCTTCTTGGAGCCAATGTACAGTCCGAGTCGGCATCTTCCACTTCTCTTCCTTAAACGAGCAGAAAGTTACTAGCAATGGCGATTTTCTTCGCCGATTCTCAAATCTCCACCACCACAGCATCCGCTCAGGACCCTTTGTTGCCTTGGCTCTGGTATTCCTCGACTACTTTTATGCGATCGAGCTTATTGACAGCCGTTCTTACATTCAATCTCAGGAAAATTGTTGGTGCTATTTGATTCCAATTGCAGGCAGTGTTTCTGGGCCAGAACTCTCCAAGCTTCTCTCTGATTGCATTGAAAATTTCAGCGGCAATGTTATGTACAAAAACGATGTTCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACTGGTTTCAAGGAATTGTTGGAGCAGGAGATATGCATTGGCAATTCTTTACTTTATATTTGGTTGGCAGCGTTCCTCGAATCCAAGGGGAGGTTAAATGACGCTAATATAGTTTATGAGCTTGGGTTGATGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAACATTGTTTATTGATAGGATATCTGAGTTGGTCAATACTCATTCAATGCAGGGGAATGATGGTAGTGAATCCACTAAATTTGCAGAAAGTTACATCAACCCTTGGTCTTCCTCCATTATGAACGATTTATTAAGCAAGATTAATCCAGAGATCATGAAGTATGATTTAGAATTGTCATCTAAATGTATAAAACTACCTTCAGTCCATCCTTCAAAAGAACGAGCTTTCTTGAAATATTGTGTAAGAAATGAAGGAAAAAGATTTCTTATGTCACAGATTCGTGGAAAGAAGTATGAGATCAAAGGCTGTGCAGGTACTGGCGGTTTTGCTCAAGTATATAAATCATATCTTGACAGCAACCCAGACCATGTAGTAGCACTGAAGATACAAACACCTCCTTTTCCTTGGGAGTTCTACATGTATCGTCAACTCGATCTTCGTGTCACCGGGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGACATGTATCTCTATTCTGACTACAGCATCCTTATATGTGATTACTTGGCTCATGGAACATTACAGGATGCCATAAATTTGTATGTAGTTCTTGGAAAGACAATGGAAGAAGTTTTATGTATTTATTACACCATAGAGATGCTCTACATGCTTGAAACTTTGCACAGCATTGGCATTATCCATGGAGATTTTAAACCTGACAATCTGCTTATTCGTTATGCGGGAGACAACCTAATAGAAGATGAATTTCAGGAACGTAGTGAATTTAAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTCCAGAAATGCTAGAGAATAAACCCTGGAAATATCAGGTCAGTTTCTTCTGCAATGTGGTTATTTATAAAAACTCTAGAGAAACCCTCTGCAAAGAAGAAAACAAAAGCTCTACCAATAGCATTCTGTCTTCTGTACAGGTAGACATATATGGCTTTTGTGTAGTCGTCCACACAATGCTTCACGGTTCCTACATGGAAGTTGAGAAAAAAACATCATCTGATGGTGACTTTGTTTATTTACCCAAATCATCTTTTAAGCGGTATTGGAACATTGAACTGTGGAAGAATCTCTTCACAAAGTTGCTTAATATCACACCAGGTGATTGCAACAAAGAGTTGTTGCAGAGTATAAGGAAATCATTCCAAGAATACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTTGTAAAGCAAAGGGCTTCCTTGTGCACTGCCTAA

Protein sequence

RSSWSQCTVRVGIFHFSSLNEQKVTSNGDFLRRFSNLHHHSIRSGPFVALALVFLDYFYAIELIDSRSYIQSQENCWCYLIPIAGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSMQGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYDLELSSKCIKLPSVHPSKERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQTPPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERSEFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRASLCTA
Homology
[Close]
BLAST of Lsi11G017000 vs. ExPASy Swiss-Prot
Match: F4IVI0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 7.3e-152
Identity = 276/542 (50.92%), Postives = 359/542 (66.24%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           AG  SG +L KLL DCI  +  +  Y+ND+RFLKIWFL +  REDFE  ++E+ E EIC 
Sbjct: 29  AGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEGREDFERVYREIEETEICK 88

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
           G+SLLY W A FLE KG    AN VY+ GL R AEP + LK+A +LF+ RIS+     S+
Sbjct: 89  GHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKEAHSLFLQRISKRTKASSL 148

Query: 204 QG-NDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-LELSSKCIKLPSVHPSKER 263
           +   D +++T     ++NPW +S +N L+ KI P+++KYD   +S+K      V P K  
Sbjct: 149 EKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKYDGYHVSNK------VFPGKAN 208

Query: 264 -AFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQTPP 323
            + L+   RN+     + +I G+KY++KGCAG GGFAQV+K+++DSNPD VVALK+Q PP
Sbjct: 209 LSSLQNYSRNK-----IIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDEVVALKVQKPP 268

Query: 324 FPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGK 383
           FPWEF+MYRQLD R+   +RSSFG A  +++YSDYSIL+CDYL+HGTLQD IN YVV+GK
Sbjct: 269 FPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQDVINSYVVVGK 328

Query: 384 TMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQER------- 443
           +MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  +NL E  F E+       
Sbjct: 329 SMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGFHEKTGSWSKK 388

Query: 444 -------------------SEFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRE 503
                              +EF GDCRTSGFRC EM E+KPWK+                
Sbjct: 389 GLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKF---------------- 448

Query: 504 TLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKR 563
                             QVD YG CV+VH MLH  YME+EKK S DG ++ +P++SFKR
Sbjct: 449 ------------------QVDTYGLCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKR 508

Query: 564 YWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRASLC 597
           YW ++LWK LFTKLLN    + + E L+++RKS +EY+CS+P+L++KL ELL KQR SLC
Sbjct: 509 YWKVDLWKELFTKLLNRETCEDDTETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLC 525

[Close]
BLAST of Lsi11G017000 vs. ExPASy Swiss-Prot
Match: O43683 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 4.3e-35
Identity = 113/400 (28.25%), Postives = 175/400 (43.75%), Query Frame = 0 

Query: 220  NPWSSSIMNDLLSKINPEIMKYDLELSSKCIKLPSVHPSKERAFLKYCVRNEGKRFLMSQ 279
            NPW   ++  LLS ++  +  Y      +C KLP++ P  E                  Q
Sbjct: 740  NPWDDKLIFKLLSGLSKPVSSYPNTFEWQC-KLPAIKPKTE-----------------FQ 799

Query: 280  IRGKKYEIKGCAGTGGFAQVYKS----YLDSNPDHVVALKIQTPPFPWEFYMYRQLDLRV 339
            +  K   +    G G FAQVY++      D+       LK+Q P  PWEFY+  QL  R+
Sbjct: 800  LGSKLVYVHHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERL 859

Query: 340  TGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEM 399
                +  F   +  +L+ + S+L+ +  ++GTL +AINLY     K M + L I + + M
Sbjct: 860  KPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRM 919

Query: 400  LYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDE----------------------FQE 459
            LYM+E +H   IIHGD KPDN ++   G+  +E +                      F +
Sbjct: 920  LYMIEQVHDCEIIHGDIKPDNFIL---GNGFLEQDDEDDLSAGLALIDLGQSIDMKLFPK 979

Query: 460  RSEFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRETLCKEENKSSTNSILSSV 519
             + F   C TSGF+C EML NKPW YQ+ +F                             
Sbjct: 980  GTIFTAKCETSGFQCVEMLSNKPWNYQIDYF----------------------------- 1039

Query: 520  QVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNIT 579
                 G    V+ ML G+YM+V+ +          P+  F+R  ++++W   F  +LNI 
Sbjct: 1040 -----GVAATVYCMLFGTYMKVKNEGGE-----CKPEGLFRRLPHLDMWNEFFHVMLNIP 1074

Query: 580  PGDCN-----KELLQSIRKSFQEYMCSNPQLIRKLKELLV 588
              DC+       L Q ++K FQ++  +    IR L+  L+
Sbjct: 1100 --DCHHLPSLDLLRQKLKKVFQQHYTNK---IRALRNRLI 1074

[Close]
BLAST of Lsi11G017000 vs. ExPASy Swiss-Prot
Match: O08901 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090 GN=Bub1 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.1e-33
Identity = 108/390 (27.69%), Postives = 173/390 (44.36%), Query Frame = 0 

Query: 220  NPWSSSIMNDLLSKINPEIMKYDLELSSKCIKLPSVHPSKERAFLKYCVRNEGKRFLMSQ 279
            NPW   ++  LLS ++  +  Y      +  KLP++    E                  Q
Sbjct: 714  NPWDDELILKLLSGLSKPVTSYSNTFEWQS-KLPAIKTKTE-----------------YQ 773

Query: 280  IRGKKYEIKGCAGTGGFAQVYKS----YLDSNPDHVVALKIQTPPFPWEFYMYRQLDLRV 339
            +      +    G G FAQV+++      ++  +    LK+Q P   WEFY+  QL  R+
Sbjct: 774  LGSLLVYVNHLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERL 833

Query: 340  TGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEM 399
              +    F   +  +L+ + SIL+ +  ++GTL + INLY     K M + L + + I M
Sbjct: 834  KPEVHHMFIKFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRM 893

Query: 400  LYMLETLHSIGIIHGDFKPDNLLIRY------------------AGDNLIEDEFQERSEF 459
            LYM+E +HS  IIHGD KPDN ++ +                   G ++    F + + F
Sbjct: 894  LYMVEQVHSCEIIHGDIKPDNFILGHRFLEQADEDLATGLALIDLGQSIDMKLFPKGTVF 953

Query: 460  KGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRETLCKEENKSSTNSILSSVQVDI 519
             G C TSGF+CPEML NKPW YQ+ +F                                 
Sbjct: 954  TGKCETSGFQCPEMLSNKPWNYQIDYF--------------------------------- 1013

Query: 520  YGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDC 579
             G    ++ ML GSYM+V+ +       V+ P+  F+R  ++++W+  F  +LNI   DC
Sbjct: 1014 -GVAATIYCMLFGSYMKVKNEGG-----VWKPEGLFRRLPHLDMWEEFFHIMLNIP--DC 1042

Query: 580  -NKELLQSIRKSFQEYMCSNPQLIRKLKEL 586
             N   L  +R++ ++ +    Q   K+K L
Sbjct: 1074 HNLPSLDFLRQNMKKLL--EQQYSNKIKTL 1042


HSP 2 Score: 51.6 bits (122), Expect = 3.6e-05
Identity = 31/104 (29.81%), Postives = 49/104 (47.12%), Query Frame = 0 

Query: 92  LSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIW 151
           L  LL   ++ F     Y ND RF+           D    F+ L  Q I   +S +Y+ 
Sbjct: 45  LMTLLEHLMKEFLHKKNYHNDSRFINYCLKFAEYNSDRHQFFEFLYNQGIGTKSSYIYMS 104

Query: 152 LAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRIS 196
            A  LE++G L  A+ +++ G+   AEP E L++   LF  R++
Sbjct: 105 WAGHLEAQGELQHASAIFQTGIHNEAEPKELLQQQYRLFQARLT 148

[Close]
BLAST of Lsi11G017000 vs. ExPASy Swiss-Prot
Match: P41695 (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BUB1 PE=1 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 3.2e-30
Identity = 104/366 (28.42%), Postives = 170/366 (46.45%), Query Frame = 0 

Query: 220 NPWSSSIMNDLLSKINPEIMKYDLELS-SKCIKLPSVHPSKERAFLKYCVRNEGKRFLMS 279
           NP S+++    LS+I+P + +Y+   + ++ +K+ S+     R       RNE K  ++ 
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKKIHRV-----SRNENKNPIVD 702

Query: 280 -QIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQTPPFPWEFYMYRQLDLRVTG 339
            +  G  Y I+G  G GG+A VY +  +S+  H+ ALK++ P   WE+Y+  Q++ R+  
Sbjct: 703 FKKTGDLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRK 762

Query: 340 KE-RSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLY----VVLGKTMEEVLCIYYTI 399
                S   A  ++L+ D S L+ +Y + GT+ D INL     +     M+E LC++ T+
Sbjct: 763 STILKSIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITV 822

Query: 400 EMLYMLETLHSIGIIHGDFKPDNLLIRY--AGDNL-------IEDEFQER---------- 459
           E++ +LE +H +GIIHGD KPDN +IR    G+ L        ED ++ +          
Sbjct: 823 ELMKVLEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRS 882

Query: 460 ---------SEFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRETLCKEENKSS 519
                    ++FK + +     C EM   KPW Y                          
Sbjct: 883 FDMTLLPPGTKFKSNWKADQQDCWEMRAGKPWSY-------------------------- 942

Query: 520 TNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKRYWNIELWKNL 551
                   + D YG   V+H+ML G ++E  +  +         K+ FKRYW  E+W  +
Sbjct: 943 --------EADYYGLAGVIHSMLFGKFIETIQLQNGRCKL----KNPFKRYWKKEIWGVI 963

[Close]
BLAST of Lsi11G017000 vs. ExPASy Swiss-Prot
Match: O94751 (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bub1 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.3e-27
Identity = 113/434 (26.04%), Postives = 179/434 (41.24%), Query Frame = 0 

Query: 219  INPWSSSIMNDLLSKINPEIMK--------------YDLELSSKCIKLPSVHPSKERAFL 278
            INP   S+ + L   + P +++                +E     IK  +  P + R+  
Sbjct: 640  INPLDQSLRDLLFQVLRPSLLRDKDYHEHETSFALVEHIESFVSKIKPKAGGPGRRRSSN 699

Query: 279  KYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVY--KSYL------------DSNPDH 338
            ++ +  +G  F +         +    G G FA VY  KS +            ++N   
Sbjct: 700  RHSL--DGPEFHLFYPPNTNLSVISKLGQGAFAPVYLVKSKIETENGDVSQGGAENNESK 759

Query: 339  VVALKIQTPPFPWEFYMYRQLDLRVTG-KERSSFGCAHDMYLYSDYSILICDYLAHGTLQ 398
            + ALKI+TPP  +EFY+ RQ   R+ G +E +S    H ++++ D S L+ DY   G++ 
Sbjct: 760  LFALKIETPPSCFEFYLTRQAMTRLKGLRETNSILPVHQLHMFHDTSHLLMDYRPQGSIL 819

Query: 399  DAINLY---VVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIR------- 458
            D +N           M+E+L ++++IE L ++E LH+  IIHGD K DN L+R       
Sbjct: 820  DLVNSMHNSTFSSSGMDEILVVFFSIEFLRIIEALHTHKIIHGDLKADNALLRLETVADS 879

Query: 459  ---------------YAGDNLIE-------DEFQERSEFKGDCRTSGFRCPEMLENKPWK 518
                           + G  LI+         F+E+ +F  D  T    C EM E +PW 
Sbjct: 880  EWSPIYSPEGLYGWSFKGIYLIDFGRGIDLSLFEEKVKFIADWDTDLQDCIEMREGRPWT 939

Query: 519  YQVSFFCNVVIYKNSRETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKK 578
            Y                                  Q+D +G   +++TML G Y+E   +
Sbjct: 940  Y----------------------------------QIDYHGLAAIIYTMLFGQYIETRIE 999

Query: 579  TSSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDCNKEL-----LQSIRKSFQEYM 587
               +G    +     KRYWN +LW  LF  LLN T     + L     L  IR   +E++
Sbjct: 1000 V-INGQRRQVLTQRMKRYWNQDLWHRLFDLLLNPTLHVSEENLPMTEELSKIRIEMEEWL 1036

[Close]
BLAST of Lsi11G017000 vs. ExPASy TrEMBL
Match: A0A5D3C9K3 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003120 PE=3 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.4e-230
Identity = 416/544 (76.47%), Postives = 434/544 (79.78%), Query Frame = 0 

Query: 84   AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
            +G+VSG EL+KLLSDCI NFSGNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 852  SGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 911

Query: 144  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
            GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 912  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 971

Query: 204  QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
            Q ND SESTKFAESYINPWSSSIMNDLL+KINP+IMKYD      +  SK + L S+  S
Sbjct: 972  QVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNS 1031

Query: 264  KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                 +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 1032 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 1091

Query: 324  PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
            PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLA+GTLQDAIN YVVL
Sbjct: 1092 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVL 1151

Query: 384  GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
            GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLL+RYAG+NL ED+FQER     
Sbjct: 1152 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQERRGPWQ 1211

Query: 444  ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                  EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 1212 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 1271

Query: 504  RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                                QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 1272 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 1331

Query: 564  KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
            KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKLKELLVKQRAS
Sbjct: 1332 KRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRAS 1347

[Close]
BLAST of Lsi11G017000 vs. ExPASy TrEMBL
Match: A0A1S3C1A0 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 GN=LOC103495479 PE=4 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.4e-230
Identity = 416/544 (76.47%), Postives = 434/544 (79.78%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           +G+VSG EL+KLLSDCI NFSGNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 37  SGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 96

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
           GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 97  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 156

Query: 204 QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
           Q ND SESTKFAESYINPWSSSIMNDLL+KINP+IMKYD      +  SK + L S+  S
Sbjct: 157 QVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNS 216

Query: 264 KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 217 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 276

Query: 324 PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
           PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLA+GTLQDAIN YVVL
Sbjct: 277 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVL 336

Query: 384 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
           GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLL+RYAG+NL ED+FQER     
Sbjct: 337 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQERRGPWQ 396

Query: 444 ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                 EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 397 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 456

Query: 504 RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                               QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 457 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 516

Query: 564 KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
           KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKLKELLVKQRAS
Sbjct: 517 KRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRAS 532

[Close]
BLAST of Lsi11G017000 vs. ExPASy TrEMBL
Match: A0A5A7SMW7 (Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003080 PE=3 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 4.1e-230
Identity = 415/544 (76.29%), Postives = 434/544 (79.78%), Query Frame = 0 

Query: 84   AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
            +G+VSG EL+KLLSDCI NFSGNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 879  SGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 938

Query: 144  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
            GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 939  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 998

Query: 204  QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
            Q ND SESTKFAESYINPWSSSIMNDLL+KINP+IMKYD      +  SK + L S+  S
Sbjct: 999  QVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNS 1058

Query: 264  KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                 +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 1059 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 1118

Query: 324  PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
            PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLA+GTLQDAIN YVVL
Sbjct: 1119 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVL 1178

Query: 384  GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
            GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLL+RYAG+NL ED+FQER     
Sbjct: 1179 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQERRGPWQ 1238

Query: 444  ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                  EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 1239 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 1298

Query: 504  RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                                QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 1299 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 1358

Query: 564  KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
            KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKL+ELLVKQRAS
Sbjct: 1359 KRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLQELLVKQRAS 1374

[Close]
BLAST of Lsi11G017000 vs. ExPASy TrEMBL
Match: A0A0A0K5T0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 9.4e-227
Identity = 411/544 (75.55%), Postives = 430/544 (79.04%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           +G+VSG EL+KLLSDCI NF GNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 37  SGNVSGSELAKLLSDCIGNFRGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 96

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
            NSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 97  SNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 156

Query: 204 QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
           Q ND SESTKFAESYINPWSSSIM+DLL+KI+P+IMKYD      +  SK + L S+  S
Sbjct: 157 QVNDVSESTKFAESYINPWSSSIMSDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNS 216

Query: 264 KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 217 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 276

Query: 324 PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
           PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLAHGTLQDAIN YVVL
Sbjct: 277 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLAHGTLQDAINSYVVL 336

Query: 384 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
           GKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNLLIRYAG+++ ED FQER     
Sbjct: 337 GKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNLLIRYAGESVTEDGFQERHGPWQ 396

Query: 444 ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                 EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 397 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 456

Query: 504 RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                               QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 457 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 516

Query: 564 KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
           KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKLKELLVKQRAS
Sbjct: 517 KRYWNIELWKNLFTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRAS 532

[Close]
BLAST of Lsi11G017000 vs. ExPASy TrEMBL
Match: A0A6J1D1C8 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 1.3e-220
Identity = 408/629 (64.86%), Postives = 452/629 (71.86%), Query Frame = 0 

Query: 2   SSWSQCTVRVGIFHFSSLNEQKVTSNGDFLRRFSNLHHHSIRSGPFVALALVFLDYFYAI 61
           SSWS+CTVRVGIFHF                               +A+A++F D     
Sbjct: 121 SSWSKCTVRVGIFHFPLFK--------------------------LLAMAIIFPD----- 180

Query: 62  ELIDSRSYIQSQENC--WCYLIPIA------GSVSGPELSKLLSDCIENFSGNVMYKNDV 121
               + S I +Q+    W + I  A      G+ SGP+L+KLLSDCI+NF GNV YKND+
Sbjct: 181 --SQNTSTISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNVKYKNDI 240

Query: 122 RFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWLAAFLESKGRLNDANIVYELGL 181
           RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIW AAFLESKG+L+DAN+VYELGL
Sbjct: 241 RFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANMVYELGL 300

Query: 182 MRNAEPLEWLKKAKTLFIDRISELVNTHSMQGNDGSESTKFAESYINPWSSSIMNDLLSK 241
           +RNAEP EWLKKAKTLFIDR+SELV+ HS Q NDG ES KFAE+Y+NPWS SIM DLLSK
Sbjct: 301 LRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIMKDLLSK 360

Query: 242 INPEIMKYDLELSSKCIKLPSVHPSKERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGT 301
           INP+I+KY+   SS          +  R      +RN  +   + +I GK+YEIKGCAGT
Sbjct: 361 INPKILKYEGYHSSS--------KAYSRKMALNSLRNSSRNKTI-EIGGKRYEIKGCAGT 420

Query: 302 GGFAQVYKSYLDSNPDHVVALKIQTPPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYS 361
           GGFAQVYK+Y+D NP+ VVALKIQTPPFPWEFYMYRQLD  VT KERSSFGCAH MY YS
Sbjct: 421 GGFAQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYS 480

Query: 362 DYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKP 421
           DYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKP
Sbjct: 481 DYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKP 540

Query: 422 DNLLIRYAGDNLIEDEFQERS--------------------------EFKGDCRTSGFRC 481
           DNLLIRYA DNL ED F+ERS                          EFKGDCRTSGFRC
Sbjct: 541 DNLLIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRC 600

Query: 482 PEMLENKPWKYQVSFFCNVVIYKNSRETLCKEENKSSTNSILSSVQVDIYGFCVVVHTML 541
            EMLENKPWKY                                  QVDIYG CVVVHTML
Sbjct: 601 TEMLENKPWKY----------------------------------QVDIYGLCVVVHTML 660

Query: 542 HGSYMEVEKKTSSDGDFVYLPKSSFKRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKS 597
           HGSYM+V+KK SS+G F+YLPK+SFKRYWNIELWKNLFTKLLNITPGD N++LLQSIR S
Sbjct: 661 HGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNLFTKLLNITPGDYNQKLLQSIRVS 673

[Close]
BLAST of Lsi11G017000 vs. NCBI nr
Match: XP_038888854.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hispida] >XP_038888855.1 mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hispida])

HSP 1 Score: 818.1 bits (2112), Expect = 4.8e-233
Identity = 420/539 (77.92%), Postives = 440/539 (81.63%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           +G+VSGPEL+KLLSDCIENFSG+V YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 37  SGNVSGPELAKLLSDCIENFSGDVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 96

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
           G+SLLYIWLA FLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM
Sbjct: 97  GHSLLYIWLAVFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 156

Query: 204 QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYDLELSSKCIKLPSVHPSKERAF 263
           Q ND SESTKFAESYINPWSSS MNDLLSKI+P+IMKYD   S       S     ++  
Sbjct: 157 QVNDVSESTKFAESYINPWSSSTMNDLLSKISPKIMKYDGYHS-------STKAYSKKMT 216

Query: 264 LKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQTPPFPW 323
           LK  +RN  +  ++ +I GKKYEIKGCAGTGGFAQVY+SYLDSNPD VVALKIQTPPFPW
Sbjct: 217 LK-SLRNSSRNKII-EIGGKKYEIKGCAGTGGFAQVYRSYLDSNPDDVVALKIQTPPFPW 276

Query: 324 EFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTME 383
           EFYMYRQLDLRV GKERSSFGCAH MYLYSDYSILICD+LAHGTLQDAIN YVVLGKTME
Sbjct: 277 EFYMYRQLDLRVNGKERSSFGCAHAMYLYSDYSILICDFLAHGTLQDAINSYVVLGKTME 336

Query: 384 EVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS--------- 443
           EVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNL EDEFQERS         
Sbjct: 337 EVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLTEDEFQERSGPWQDQGLC 396

Query: 444 -----------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRETLC 503
                            EFKGDCRTSGFRCPEMLENKPWK+                   
Sbjct: 397 LVDWGRGIDLHLFPENVEFKGDCRTSGFRCPEMLENKPWKF------------------- 456

Query: 504 KEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKRYWN 563
                          QVDIYGFCVVVHTMLHGSYMEVEKK SSDGDFVYLPK+SFKRYWN
Sbjct: 457 ---------------QVDIYGFCVVVHTMLHGSYMEVEKKPSSDGDFVYLPKTSFKRYWN 516

Query: 564 IELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRASLCTA 597
           IELWKNLFTKLLNITPGD NKEL+QSIRKSFQ+YMCSNPQLIRKLKELL KQRASLC A
Sbjct: 517 IELWKNLFTKLLNITPGDYNKELIQSIRKSFQDYMCSNPQLIRKLKELLAKQRASLCNA 532

[Close]
BLAST of Lsi11G017000 vs. NCBI nr
Match: TYK06996.1 (protein TPX2 [Cucumis melo var. makuwa])

HSP 1 Score: 808.9 bits (2088), Expect = 2.9e-230
Identity = 416/544 (76.47%), Postives = 434/544 (79.78%), Query Frame = 0 

Query: 84   AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
            +G+VSG EL+KLLSDCI NFSGNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 852  SGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 911

Query: 144  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
            GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 912  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 971

Query: 204  QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
            Q ND SESTKFAESYINPWSSSIMNDLL+KINP+IMKYD      +  SK + L S+  S
Sbjct: 972  QVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNS 1031

Query: 264  KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                 +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 1032 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 1091

Query: 324  PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
            PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLA+GTLQDAIN YVVL
Sbjct: 1092 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVL 1151

Query: 384  GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
            GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLL+RYAG+NL ED+FQER     
Sbjct: 1152 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQERRGPWQ 1211

Query: 444  ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                  EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 1212 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 1271

Query: 504  RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                                QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 1272 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 1331

Query: 564  KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
            KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKLKELLVKQRAS
Sbjct: 1332 KRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRAS 1347

[Close]
BLAST of Lsi11G017000 vs. NCBI nr
Match: XP_008455277.1 (PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo])

HSP 1 Score: 808.9 bits (2088), Expect = 2.9e-230
Identity = 416/544 (76.47%), Postives = 434/544 (79.78%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           +G+VSG EL+KLLSDCI NFSGNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 37  SGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 96

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
           GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 97  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 156

Query: 204 QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
           Q ND SESTKFAESYINPWSSSIMNDLL+KINP+IMKYD      +  SK + L S+  S
Sbjct: 157 QVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNS 216

Query: 264 KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 217 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 276

Query: 324 PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
           PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLA+GTLQDAIN YVVL
Sbjct: 277 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVL 336

Query: 384 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
           GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLL+RYAG+NL ED+FQER     
Sbjct: 337 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQERRGPWQ 396

Query: 444 ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                 EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 397 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 456

Query: 504 RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                               QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 457 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 516

Query: 564 KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
           KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKLKELLVKQRAS
Sbjct: 517 KRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRAS 532

[Close]
BLAST of Lsi11G017000 vs. NCBI nr
Match: KAA0031543.1 (protein TPX2 [Cucumis melo var. makuwa])

HSP 1 Score: 807.4 bits (2084), Expect = 8.4e-230
Identity = 415/544 (76.29%), Postives = 434/544 (79.78%), Query Frame = 0 

Query: 84   AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
            +G+VSG EL+KLLSDCI NFSGNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 879  SGNVSGSELAKLLSDCIGNFSGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 938

Query: 144  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
            GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 939  GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 998

Query: 204  QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
            Q ND SESTKFAESYINPWSSSIMNDLL+KINP+IMKYD      +  SK + L S+  S
Sbjct: 999  QVNDVSESTKFAESYINPWSSSIMNDLLNKINPKIMKYDGYHSSTKAYSKKMNLKSLRNS 1058

Query: 264  KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                 +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 1059 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 1118

Query: 324  PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
            PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLA+GTLQDAIN YVVL
Sbjct: 1119 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLANGTLQDAINSYVVL 1178

Query: 384  GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
            GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLL+RYAG+NL ED+FQER     
Sbjct: 1179 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLVRYAGENLTEDDFQERRGPWQ 1238

Query: 444  ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                  EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 1239 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 1298

Query: 504  RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                                QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 1299 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 1358

Query: 564  KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
            KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKL+ELLVKQRAS
Sbjct: 1359 KRYWNIELWKNLFTKLLNITPGQYNKELLQSLRKSFQDYMCSNPQLIRKLQELLVKQRAS 1374

[Close]
BLAST of Lsi11G017000 vs. NCBI nr
Match: XP_004136844.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43647.1 hypothetical protein Csa_017323 [Cucumis sativus])

HSP 1 Score: 796.2 bits (2055), Expect = 1.9e-226
Identity = 411/544 (75.55%), Postives = 430/544 (79.04%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           +G+VSG EL+KLLSDCI NF GNV YKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI
Sbjct: 37  SGNVSGSELAKLLSDCIGNFRGNVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 96

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
            NSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAK LFIDRISELVNTHSM
Sbjct: 97  SNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKRLFIDRISELVNTHSM 156

Query: 204 QGNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-----LELSSKCIKLPSVHPS 263
           Q ND SESTKFAESYINPWSSSIM+DLL+KI+P+IMKYD      +  SK + L S+  S
Sbjct: 157 QVNDVSESTKFAESYINPWSSSIMSDLLNKISPKIMKYDGYHSSTKAYSKKMNLKSLRNS 216

Query: 264 KERAFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQT 323
                +              +I GKKYEIKGCAGTGGFAQVYKSYLDSNPD VVALKIQT
Sbjct: 217 SRNKTI--------------EIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQT 276

Query: 324 PPFPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVL 383
           PPFPWEFYMYRQLDLRV  KERSSFGCAH MYLYSDYSILICDYLAHGTLQDAIN YVVL
Sbjct: 277 PPFPWEFYMYRQLDLRVAAKERSSFGCAHAMYLYSDYSILICDYLAHGTLQDAINSYVVL 336

Query: 384 GKTMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQERS---- 443
           GKTMEEVLCIYYTIEMLYMLETLHSI IIHGDFKPDNLLIRYAG+++ ED FQER     
Sbjct: 337 GKTMEEVLCIYYTIEMLYMLETLHSIDIIHGDFKPDNLLIRYAGESVTEDGFQERHGPWQ 396

Query: 444 ----------------------EFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNS 503
                                 EFKGDCRTSGFRCPEMLEN+PWKY              
Sbjct: 397 DQGLCLVDWGRGIDLQLFPENVEFKGDCRTSGFRCPEMLENRPWKY-------------- 456

Query: 504 RETLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSF 563
                               QVDIYG CVVVHTMLHGSYMEVEKK SSDGDFVYLPKSSF
Sbjct: 457 --------------------QVDIYGLCVVVHTMLHGSYMEVEKKASSDGDFVYLPKSSF 516

Query: 564 KRYWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRAS 597
           KRYWNIELWKNLFTKLLNITPG  NKELLQS+RKSFQ+YMCSNPQLIRKLKELLVKQRAS
Sbjct: 517 KRYWNIELWKNLFTKLLNITPGHYNKELLQSLRKSFQDYMCSNPQLIRKLKELLVKQRAS 532

[Close]
BLAST of Lsi11G017000 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 538.9 bits (1387), Expect = 5.2e-153
Identity = 276/542 (50.92%), Postives = 359/542 (66.24%), Query Frame = 0 

Query: 84  AGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICI 143
           AG  SG +L KLL DCI  +  +  Y+ND+RFLKIWFL +  REDFE  ++E+ E EIC 
Sbjct: 29  AGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEGREDFERVYREIEETEICK 88

Query: 144 GNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTHSM 203
           G+SLLY W A FLE KG    AN VY+ GL R AEP + LK+A +LF+ RIS+     S+
Sbjct: 89  GHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKEAHSLFLQRISKRTKASSL 148

Query: 204 QG-NDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYD-LELSSKCIKLPSVHPSKER 263
           +   D +++T     ++NPW +S +N L+ KI P+++KYD   +S+K      V P K  
Sbjct: 149 EKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKYDGYHVSNK------VFPGKAN 208

Query: 264 -AFLKYCVRNEGKRFLMSQIRGKKYEIKGCAGTGGFAQVYKSYLDSNPDHVVALKIQTPP 323
            + L+   RN+     + +I G+KY++KGCAG GGFAQV+K+++DSNPD VVALK+Q PP
Sbjct: 209 LSSLQNYSRNK-----IIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDEVVALKVQKPP 268

Query: 324 FPWEFYMYRQLDLRVTGKERSSFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGK 383
           FPWEF+MYRQLD R+   +RSSFG A  +++YSDYSIL+CDYL+HGTLQD IN YVV+GK
Sbjct: 269 FPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQDVINSYVVVGK 328

Query: 384 TMEEVLCIYYTIEMLYMLETLHSIGIIHGDFKPDNLLIRYAGDNLIEDEFQER------- 443
           +MEEVLC+YYTIEML MLETLHS+GIIHGDFKPDNLLIRY  +NL E  F E+       
Sbjct: 329 SMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGFHEKTGSWSKK 388

Query: 444 -------------------SEFKGDCRTSGFRCPEMLENKPWKYQVSFFCNVVIYKNSRE 503
                              +EF GDCRTSGFRC EM E+KPWK+                
Sbjct: 389 GLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKF---------------- 448

Query: 504 TLCKEENKSSTNSILSSVQVDIYGFCVVVHTMLHGSYMEVEKKTSSDGDFVYLPKSSFKR 563
                             QVD YG CV+VH MLH  YME+EKK S DG ++ +P++SFKR
Sbjct: 449 ------------------QVDTYGLCVIVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKR 508

Query: 564 YWNIELWKNLFTKLLNITPGDCNKELLQSIRKSFQEYMCSNPQLIRKLKELLVKQRASLC 597
           YW ++LWK LFTKLLN    + + E L+++RKS +EY+CS+P+L++KL ELL KQR SLC
Sbjct: 509 YWKVDLWKELFTKLLNRETCEDDTETLRNLRKSMEEYICSDPKLMKKLNELLAKQRISLC 525

[Close]
BLAST of Lsi11G017000 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 67.8 bits (164), Expect = 3.4e-11
Identity = 46/168 (27.38%), Postives = 79/168 (47.02%), Query Frame = 0 

Query: 82  PIAGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEI 141
           P  G  SG  L  +   C+  F  +  YK+D+R+LK+W        D E  +K L   EI
Sbjct: 97  PPGGECSG--LLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEHCADAEVIYKFLEVNEI 156

Query: 142 CIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTH 201
              +++ YI  A  +E K ++  AN ++ LG+ R+A+P+E L  A   F+ R     NT 
Sbjct: 157 GKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLNDAYKKFMVRTMRRSNTA 216

Query: 202 SMQGNDGSE-STKFAESYINPWSSSIMNDLLSKINPEIMKYDLELSSK 249
             +  + ++  ++   + ++   ++     L   NP+  K     SSK
Sbjct: 217 DEEPKENNDLPSRSFGTLLSRGDNNARRQALGSSNPQAKKLKPNQSSK 262

[Close]
BLAST of Lsi11G017000 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 67.8 bits (164), Expect = 3.4e-11
Identity = 50/170 (29.41%), Postives = 78/170 (45.88%), Query Frame = 0 

Query: 82  PIAGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEI 141
           P  G  SG  L  +   C+  F  +  YK+D+R+LK+W        D E  +K L   EI
Sbjct: 97  PPGGECSG--LLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEHCADAEVIYKFLEVNEI 156

Query: 142 CIGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRISELVNTH 201
              +++ YI  A  +E K ++  AN ++ LG+ R+A+P+E L  A   F+ R     NT 
Sbjct: 157 GKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLNDAYKKFMVRTMRRSNTA 216

Query: 202 SMQ---GNDGSESTKFAESYINPWSSSIMNDLLSKINPEIMKYDLELSSK 249
             Q    N+   S  F  + ++   ++     L   NP+  K     SSK
Sbjct: 217 DEQEPKENNDLPSRSFG-TLLSRGDNNARRQALGSSNPQAKKLKPNQSSK 263

[Close]
BLAST of Lsi11G017000 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 63.2 bits (152), Expect = 8.4e-10
Identity = 31/112 (27.68%), Postives = 54/112 (48.21%), Query Frame = 0 

Query: 83  IAGSVSGPELSKLLSDCIENFSGNVMYKNDVRFLKIWFLSIGMREDFETGFKELLEQEIC 142
           +   +   +L + L  C E+F  +  YKND R++++W   +   +D     + +  + I 
Sbjct: 42  LPSQILNEKLPRFLQKCAESFESDKRYKNDSRYIRVWLQLMDFVDDPRALLRTMEAKSIG 101

Query: 143 IGNSLLYIWLAAFLESKGRLNDANIVYELGLMRNAEPLEWLKKAKTLFIDRI 195
              SL Y   A   E   R  DA  +Y LG+   AEP++ L+K+   F+ R+
Sbjct: 102 TKRSLFYQAYALHYEKMKRFEDAEKMYRLGVQNLAEPMDELQKSYLQFVTRM 153

[Close]
BLAST of Lsi11G017000 vs. TAIR 10
Match: AT1G45160.1 (Protein kinase superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-05
Identity = 33/89 (37.08%), Postives = 47/89 (52.81%), Query Frame = 0 

Query: 342 SFGCAHDMYLYSDYSILICDYLAHGTLQDAINLYVVLGKT--MEEVLCIYYTIEMLYMLE 401
           SF C  ++Y       L+ +YL  G      +LY +L K   ++E +   Y  E++  LE
Sbjct: 736 SFTCRDNLY-------LVMEYLNGG------DLYSLLQKVGCLDEEIARIYIAELVLALE 795

Query: 402 TLHSIGIIHGDFKPDNLLIRYAGDNLIED 429
            LHS+ I+H D KPDNLLI Y G   + D
Sbjct: 796 YLHSLKIVHRDLKPDNLLIAYNGHIKLTD 811

The following BLAST results are available for this feature:
BLAST of Lsi11G017000 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4IVI07.3e-15250.92Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ... [more]
O436834.3e-3528.25Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 ... [more]
O089011.1e-3327.69Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090... [more]
P416953.2e-3028.42Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (str... [more]
O947514.3e-2726.04Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (st... [more]
BLAST of Lsi11G017000 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3C9K31.4e-23076.47Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003120 P... [more]
A0A1S3C1A01.4e-23076.47mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7SMW74.1e-23076.29Protein TPX2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G003080 ... [more]
A0A0A0K5T09.4e-22775.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G050780 PE=4 SV=1[more]
A0A6J1D1C81.3e-22064.86mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Momordica ... [more]
BLAST of Lsi11G017000 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038888854.14.8e-23377.92mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 [Benincasa hi... [more]
TYK06996.12.9e-23076.47protein TPX2 [Cucumis melo var. makuwa][more]
XP_008455277.12.9e-23076.47PREDICTED: mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis melo... [more]
KAA0031543.18.4e-23076.29protein TPX2 [Cucumis melo var. makuwa][more]
XP_004136844.11.9e-22675.55mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucumis sativus] >KGN43... [more]
BLAST of Lsi11G017000 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G20635.15.2e-15350.92ATP binding;protein kinases;protein serine/threonine kinases [more]
AT2G33560.13.4e-1127.38BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT2G33560.23.4e-1129.41BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT5G05510.18.4e-1027.68Mad3/BUB1 homology region 1 [more]
AT1G45160.11.3e-0537.08Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 285..568
e-value: 1.4E-7
score: -27.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 287..488
e-value: 2.1E-12
score: 47.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 285..596
score: 14.831527
IPR013212Mad3/Bub1 homology region 1SMARTSM00777mad3_bub1_icoord: 50..173
e-value: 9.9E-8
score: 18.3
IPR013212Mad3/Bub1 homology region 1PFAMPF08311Mad3_BUB1_Icoord: 91..173
e-value: 9.0E-17
score: 61.2
IPR013212Mad3/Bub1 homology region 1PROSITEPS51489BUB1_Ncoord: 57..211
score: 24.165354
NoneNo IPR availableGENE3D1.25.40.430coord: 68..210
e-value: 2.4E-26
score: 94.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 282..593
e-value: 1.7E-77
score: 261.9
NoneNo IPR availablePANTHERPTHR14030:SF4BUB1 KINASE, ISOFORM A-RELATEDcoord: 202..595
coord: 87..197
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 87..197
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 202..595
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 406..418
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 277..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G017000.1Lsi11G017000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0051754 meiotic sister chromatid cohesion, centromeric
biological_process GO:0007094 mitotic spindle assembly checkpoint signaling
biological_process GO:0060236 regulation of mitotic spindle organization
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000776 kinetochore
cellular_component GO:0005874 microtubule
cellular_component GO:0005634 nucleus
cellular_component GO:0005819 spindle
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity