Lsi11G016150 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G016150
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	GDSL esterase/lipase At5g03980-like
Location	chr11: 24474466 .. 24475827 (-)
RNA-Seq Expression	Lsi11G016150
Synteny	Lsi11G016150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCACTGGTCGTTGTTCTAATGGCTTACTAATCATCGATCAATTTGGTATGTATTATTCTTAAACCAAATGATTATCAAACGGAACCTTTATTTTTTTATGATTTAGTTTTTTTTACAAATTAGCTCATTTCTTTTATGATTATCTTATAGCTTTTGCAGCTGGACTTCCTTTGATAAACCCATATTTGAATAAAGAGGCACCGATGAGTCAAGGGGTGAATTTTGCTGTGGCTGGTTCTACAGCTTTACCTTCTGAAATTCTTGCTGAGAAAATGATATATTCTCCTGTCACTAATTCATCTTTGACTAAACAACTTGATTGGATGGCTACTCATTTTAACACTACTTGTTCTCATCAAAGAGGTTTGTGTGTGTGTATATACTTCCTTTTGTCATTATATTAAACTTGCATTTATGTATATATTATTCTATATGTACAAATTCTCTTTTCAATTATAATTATAATTACTCATCTCCAATGTAACTTCCAATGTTTATTTTACAGATTGTACCGAGAAATTAAAGAATGCTTTGTTTTTTGTTGGTGAGATTGGAGGGAATGATTACAACTATGCTTTATTTCAAGGAAAGAGCATAGAAGAAGTTGAAAATATGGTTCCTGAGGTTGTTCAAACCATAAAGGAAGCTGTCACTGTATGTCTTGCTCAATTTTGTTTAATTTTTTGTGAATTATATTTATTTTTACGTTAACTAGAAGTTCTTGTAAAAATAAAGACAAAAAAGACTTGAAGTTGTTATCTAATTTGCTTTTCTTTGATCATAGCAGAGAGTCATAAGTTATGGTGCTACTCGAGTTATTGTGCCAGGAAACTTTCCAATAGGTTGCATGTCAATCTATCTCACTGGATTCAAAACTAAGGATTACAATGCTTATGATGAGCTTCATTGTTTGAAGAATTTGAACAATTTTGCCCGCTATCACAATTACCATCTTAAAGACGCTATTAGATCCTTGAGAAAAGATAACCCAAATGTAATGATCGTGTATGGTGATTATTATAATGCACTATTGTGGGTTCTACGACATGCTTCCTTACTTGGTAAGCTTATTTATACACTATATAATATATGTTTGTTTCTTGCTTTATGATCTAATATATTTATGCAATCTCTATTCTAGGTTTTGATGAAACATCTTTGCAAAAGTCAGGTTGTGGGATGGGCGGTGATTATGACTTCAACTTAATGCAAATGTGTGGACTTCCTGGAGTACCTGTTTGTCCTAATCCTGAAGAACGAATTAGTTGGGACGGAGTTCATTTGACCCAAGAGACTTACAAATATATGACATATTGGCTCATTCATGACATATTTCCAAAGCTTCATTGCATAGTTTAA

mRNA sequence

ATGCCACTGGCACCGATGAGTCAAGGGGTGAATTTTGCTGTGGCTGGTTCTACAGCTTTACCTTCTGAAATTCTTGCTGAGAAAATGATATATTCTCCTGTCACTAATTCATCTTTGACTAAACAACTTGATTGGATGGCTACTCATTTTAACACTACTTGTTCTCATCAAAGAGATTGTACCGAGAAATTAAAGAATGCTTTGTTTTTTGTTGGTGAGATTGGAGGGAATGATTACAACTATGCTTTATTTCAAGGAAAGAGCATAGAAGAAGTTGAAAATATGGTTCCTGAGGTTGTTCAAACCATAAAGGAAGCTGTCACTAGAGTCATAAGTTATGGTGCTACTCGAGTTATTGTGCCAGGAAACTTTCCAATAGGTTGCATGTCAATCTATCTCACTGGATTCAAAACTAAGGATTACAATGCTTATGATGAGCTTCATTGTTTGAAGAATTTGAACAATTTTGCCCGCTATCACAATTACCATCTTAAAGACGCTATTAGATCCTTGAGAAAAGATAACCCAAATGTAATGATCGTGTATGGTTTTGATGAAACATCTTTGCAAAAGTCAGGTTGTGGGATGGGCGGTGATTATGACTTCAACTTAATGCAAATGTGTGGACTTCCTGGAGTACCTGTTTGTCCTAATCCTGAAGAACGAATTAGTTGGGACGGAGTTCATTTGACCCAAGAGACTTACAAATATATGACATATTGGCTCATTCATGACATATTTCCAAAGCTTCATTGCATAGTTTAA

Coding sequence (CDS)

ATGCCACTGGCACCGATGAGTCAAGGGGTGAATTTTGCTGTGGCTGGTTCTACAGCTTTACCTTCTGAAATTCTTGCTGAGAAAATGATATATTCTCCTGTCACTAATTCATCTTTGACTAAACAACTTGATTGGATGGCTACTCATTTTAACACTACTTGTTCTCATCAAAGAGATTGTACCGAGAAATTAAAGAATGCTTTGTTTTTTGTTGGTGAGATTGGAGGGAATGATTACAACTATGCTTTATTTCAAGGAAAGAGCATAGAAGAAGTTGAAAATATGGTTCCTGAGGTTGTTCAAACCATAAAGGAAGCTGTCACTAGAGTCATAAGTTATGGTGCTACTCGAGTTATTGTGCCAGGAAACTTTCCAATAGGTTGCATGTCAATCTATCTCACTGGATTCAAAACTAAGGATTACAATGCTTATGATGAGCTTCATTGTTTGAAGAATTTGAACAATTTTGCCCGCTATCACAATTACCATCTTAAAGACGCTATTAGATCCTTGAGAAAAGATAACCCAAATGTAATGATCGTGTATGGTTTTGATGAAACATCTTTGCAAAAGTCAGGTTGTGGGATGGGCGGTGATTATGACTTCAACTTAATGCAAATGTGTGGACTTCCTGGAGTACCTGTTTGTCCTAATCCTGAAGAACGAATTAGTTGGGACGGAGTTCATTTGACCCAAGAGACTTACAAATATATGACATATTGGCTCATTCATGACATATTTCCAAAGCTTCATTGCATAGTTTAA

Protein sequence

MPLAPMSQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKLKNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYGFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHLTQETYKYMTYWLIHDIFPKLHCIV

Homology

[Close]

BLAST of Lsi11G016150 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-62
Identity = 124/258 (48.06%), Postives = 161/258 (62.40%), Query Frame = 0


Query: 10  VNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTEKLKNAL 69
           VNF V+GSTAL S   +E+ ++ P TN+ L+ QL W   H  +TC     DC   LK++L
Sbjct: 66  VNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSL 125

Query: 70  FFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIGC 129
           F VGEIGGNDYNY  FQGK +EE+ + +P VV  I  A   VI  GA  V+VPGNFP+GC
Sbjct: 126 FMVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGC 185

Query: 130 MSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYG----- 189
             IYLT F  KD   YD+  CL +LN FA  HN  L++AI SLRK+ P+V IVYG     
Sbjct: 186 FPIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNA 245

Query: 190 ---------FDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHLTQET 249
                    FD++   KS CG GG Y+++  +  G  GVPVC NP + ISWDGVHLTQ+ 
Sbjct: 246 FQYVLRSERFDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKA 305

Query: 250 YKYMTYWLIHDIFPKLHC 253
           Y++M+ +L + I  ++ C
Sbjct: 306 YRFMSKFLNNQILSQIKC 320

[Close]

BLAST of Lsi11G016150 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 203.4 bits (516), Expect = 3.1e-51
Identity = 107/258 (41.47%), Postives = 153/258 (59.30%), Query Frame = 0


Query: 4   APMSQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEK 63
           A   +GVNFAVAG+TAL    L E+ I+S +TN SL+ QL          C    DC + 
Sbjct: 107 ANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDM 166

Query: 64  LKNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGN 123
           ++NAL  +GEIGGNDYN+ALFQ K ++EVE +VP V+ TI  A+T ++  G    +VPGN
Sbjct: 167 IENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGN 226

Query: 124 FPIGCMSIYLTGFKTKDYNAYDEL-HCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY 183
           FPIG  + YLT +KT +   YD L  CLK LN+F+ Y+N  L++ +  LRK  P+V I+Y
Sbjct: 227 FPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIY 286

Query: 184 -----------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISW 243
                            GF    L  + CG+GG Y+FN  + CG  GV  C +P + +++
Sbjct: 287 ADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNY 346

[Close]

BLAST of Lsi11G016150 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 3.8e-49
Identity = 105/261 (40.23%), Postives = 146/261 (55.94%), Query Frame = 0


Query: 9   GVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKLKNAL 68
           GVNFAVAG+TAL    LA + +     +S L+ QL+W  T+  + CS  ++C+ KLKNAL
Sbjct: 104 GVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNAL 163

Query: 69  FFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIGC 128
           F +G IG ND NYA F  ++IEE+   VP + + +  A   +I  G +RVIVPG FPIGC
Sbjct: 164 FILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATREIIRLGGSRVIVPGIFPIGC 223

Query: 129 MSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY------ 188
           ++  L           D+L CL +LNN + Y N   + A+ SL  + P  +I+Y      
Sbjct: 224 VARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNA 283

Query: 189 -----------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHLT 248
                      G + TSL K  CG+GG Y+++  + CG  GVPVCPNP + I WDG H T
Sbjct: 284 WRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFT 343

Query: 249 QETYKYMTYWLIHDIFPKLHC 253
           Q  Y+ +  ++I  I   L C
Sbjct: 344 QAAYRRVAEYVIPGIIKALKC 363

[Close]

BLAST of Lsi11G016150 vs. ExPASy Swiss-Prot
Match: Q3ZFI4 (Sinapine esterase OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 5.5e-48
Identity = 104/255 (40.78%), Postives = 146/255 (57.25%), Query Frame = 0


Query: 8   QGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTEKLKN 67
           QGVNFAV G+TAL      EK I S  TN SL+ QL+       T C S  RDC E L +
Sbjct: 109 QGVNFAVYGATALDRAFFIEKGIVSDFTNVSLSVQLNTFKQILPTLCASSSRDCREMLGD 168

Query: 68  ALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPI 127
           +L  +GE GGNDYNY  F+ KSI E++ + P +++ I +A+  +I  G    +VPG+FP+
Sbjct: 169 SLILMGESGGNDYNYPFFEDKSINEIKELTPLIIKAISDAIVDLIDLGGKTFLVPGSFPV 228

Query: 128 GCMSIYLTGFKTKDYNAYDEL-HCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYGFD 187
           GC + YLT F+T     YD L  CL  LN+F ++H+  LK  IR LRK  P+V I+Y   
Sbjct: 229 GCSAAYLTLFQTAKEKDYDPLTGCLPWLNDFGKHHDEQLKTEIRRLRKLYPHVNIMYADY 288

Query: 188 ETSLQK----------------SGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVH 245
             SL +                + CG+GG Y+F + + CG  GV  C NP E I+WDG H
Sbjct: 289 YNSLYRLYQKPTKYGFKNRPLAACCGVGGQYNFTIGEECGYEGVGYCQNPSEYINWDGYH 348

[Close]

BLAST of Lsi11G016150 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 7.2e-48
Identity = 103/248 (41.53%), Postives = 139/248 (56.05%), Query Frame = 0


Query: 8   QGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKLKNA 67
           +GVNFAVAG+TAL S  L ++ I  P TN SL  QL          C    DC + + NA
Sbjct: 106 KGVNFAVAGATALKSSFLKKRGI-QPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNA 165

Query: 68  LFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIG 127
           L  +GEIGGNDYN+  F  K ++EVE +VP V+ +I   +T +I  G    +VPG FPIG
Sbjct: 166 LILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIG 225

Query: 128 CMSIYLTGFKTKDYNAYD-ELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYGFDE 187
           C  +YLT +KT + + YD    CLK LN F  YH+  LK  +  LRK  P+V I+Y    
Sbjct: 226 CSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYY 285

Query: 188 TSLQK----------------SGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHL 239
            SL +                + CG+GG Y+FN  + CG  GV  C +P + + WDGVH+
Sbjct: 286 NSLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHM 345

[Close]

BLAST of Lsi11G016150 vs. ExPASy TrEMBL
Match: A0A6J1GKN7 (acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111454872 PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.3e-104
Identity = 191/268 (71.27%), Postives = 213/268 (79.48%), Query Frame = 0


Query: 4   APMSQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTE 63
           APM+ GVNFAVAGST LPSE+LA+K I S VTNSSL+KQLDWM+THFN TC   + DCT+
Sbjct: 101 APMTHGVNFAVAGSTPLPSEVLAQKRISSRVTNSSLSKQLDWMSTHFNITCLFKENDCTK 160

Query: 64  KLKNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPG 123
           KLKNALFFVGEIGGNDYNYALFQGK+IEEV NMVPEVVQ IKEAV RVI+YGA +VIVPG
Sbjct: 161 KLKNALFFVGEIGGNDYNYALFQGKTIEEVRNMVPEVVQIIKEAVERVINYGAFQVIVPG 220

Query: 124 NFPIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY 183
           NFPIGC+ I+L GF+T D  AYDELHCLK+LNN A YHN HL DAI+S RK+NPNV IVY
Sbjct: 221 NFPIGCLPIFLAGFQTDDATAYDELHCLKDLNNLAIYHNTHLNDAIKSFRKENPNVNIVY 280

Query: 184 -----------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISW 243
                            GFDETSL K  CGM GDY+FNLMQMCG PGVPVC NP ERISW
Sbjct: 281 GDYYNALLWVLREASSLGFDETSLHKPCCGMDGDYEFNLMQMCGAPGVPVCGNPGERISW 340

Query: 244 DGVHLTQETYKYMTYWLIHDIFPKLHCI 254
           DG HLTQ  Y+ +TYWLI +I P+L+CI
Sbjct: 341 DGFHLTQNAYRLVTYWLILNILPRLYCI 368

[Close]

BLAST of Lsi11G016150 vs. ExPASy TrEMBL
Match: A0A1S4E629 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.8e-102
Identity = 184/267 (68.91%), Postives = 212/267 (79.40%), Query Frame = 0


Query: 6   MSQGVNFAVAGSTALPSEILAEK-MIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKL 65
           M  GVNFAVAGSTAL S+ L+ K  I SPVTNSSL  QL+WM +HFN+ C +QRDC EKL
Sbjct: 104 MDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKL 163

Query: 66  KNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNF 125
           ++ALFFVGEIGGNDYNYALFQGK+I+E ++MVP+VVQTIK AV +VISYGATRV+VPGNF
Sbjct: 164 RSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNF 223

Query: 126 PIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY-- 185
           PIGC  IYLTGF T D +AYDELHCLK+LN FA YHN  +K  I  L+K+NP+ +IVY  
Sbjct: 224 PIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGD 283

Query: 186 ---------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDG 245
                          GFDE SLQKS CG+GGDY+FNLMQMCG+ GV  CPNP E ISWDG
Sbjct: 284 YYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDG 343

Query: 246 VHLTQETYKYMTYWLIHDIFPKLHCIV 255
           VHLTQ+TYK+MT+WLIHDIFPKLHCIV
Sbjct: 344 VHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of Lsi11G016150 vs. ExPASy TrEMBL
Match: A0A6J1KFS3 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 4.0e-102
Identity = 178/263 (67.68%), Postives = 215/263 (81.75%), Query Frame = 0


Query: 9   GVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKLKNAL 68
           GVNFAVAGSTAL S++L++K I SPVTNSSL +QL WM +HFN+ C +QRDC EKL+NAL
Sbjct: 112 GVNFAVAGSTALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNAL 171

Query: 69  FFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIGC 128
           F VGEIGGNDYNYALFQGK+I++V++MVP+VVQTIK AV +VISYGATRV+VPGNFPIGC
Sbjct: 172 FLVGEIGGNDYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGC 231

Query: 129 MSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY------ 188
           + IYLTGF+T D  AYDELHCLK+LN  A YHN  +K+AI  L+++NP+ MIVY      
Sbjct: 232 LPIYLTGFQTNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNA 291

Query: 189 -----------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHLT 248
                      GFDETSLQKS CG+GGDY+F+LM+MCG+ GV VC NP+ER+SWDG+HLT
Sbjct: 292 LLWILRRAFILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCXNPDERVSWDGIHLT 351

Query: 249 QETYKYMTYWLIHDIFPKLHCIV 255
           Q+ YK+M YWLIHDI P+LHCIV
Sbjct: 352 QKAYKFMAYWLIHDILPQLHCIV 374

[Close]

BLAST of Lsi11G016150 vs. ExPASy TrEMBL
Match: A0A5D3CNR6 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00130 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.9e-102
Identity = 183/267 (68.54%), Postives = 212/267 (79.40%), Query Frame = 0


Query: 6   MSQGVNFAVAGSTALPSEILAEK-MIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKL 65
           M  GVNFAVAGSTAL S+ L+ K  I SPVTNSSL  QL+WM +HFN+ C +QRDC EKL
Sbjct: 104 MDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKL 163

Query: 66  KNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNF 125
           ++ALFFVGEIGGNDYNYALFQGK+I+E ++MVP+VVQTIK AV +VISYGATRV+VPGNF
Sbjct: 164 RSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNF 223

Query: 126 PIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY-- 185
           PIGC  IYLTGF T D +AYDELHCLK+LN FA +HN  +K  I  L+K+NP+ +IVY  
Sbjct: 224 PIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATHHNDQIKQTIEVLKKENPHTVIVYGD 283

Query: 186 ---------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDG 245
                          GFDE SLQKS CG+GGDY+FNLMQMCG+ GV  CPNP E ISWDG
Sbjct: 284 YYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDG 343

Query: 246 VHLTQETYKYMTYWLIHDIFPKLHCIV 255
           VHLTQ+TYK+MT+WLIHDIFPKLHCIV
Sbjct: 344 VHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of Lsi11G016150 vs. ExPASy TrEMBL
Match: A0A6J1K9I7 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493500 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.2e-101
Identity = 177/263 (67.30%), Postives = 214/263 (81.37%), Query Frame = 0


Query: 9   GVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKLKNAL 68
           GVNFAVAGSTAL S++L++K I SPVTNSSL +QL WM +HFN+ C +QRDC EKL+NAL
Sbjct: 112 GVNFAVAGSTALSSQLLSQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNAL 171

Query: 69  FFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIGC 128
           F VGEIGGNDYNYALFQGK+I++V++MVP+VVQTIK AV + ISYGATRV+VPGNFPIGC
Sbjct: 172 FLVGEIGGNDYNYALFQGKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGC 231

Query: 129 MSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY------ 188
           + IYLTGF+T D  AYDELHCLK+LN  A YHN  +K+AI  L+++NP+ MIVY      
Sbjct: 232 LPIYLTGFQTNDTTAYDELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNA 291

Query: 189 -----------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHLT 248
                      GFDETSLQKS CG+GGDY+F+LM+MCG+ GV VC NP+ER+SWDG+HLT
Sbjct: 292 LLWILRRAFILGFDETSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLT 351

Query: 249 QETYKYMTYWLIHDIFPKLHCIV 255
           Q+ YK+M YWLIHDI P+LHCIV
Sbjct: 352 QKAYKFMAYWLIHDILPQLHCIV 374

[Close]

BLAST of Lsi11G016150 vs. NCBI nr
Match: XP_023553636.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 396.0 bits (1016), Expect = 2.5e-106
Identity = 195/268 (72.76%), Postives = 216/268 (80.60%), Query Frame = 0


Query: 4   APMSQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTE 63
           APM+ GVNFAVAGSTALPSE+LA+K I S VTNSSL+KQLDWM+THFN TC   + DCT+
Sbjct: 106 APMTHGVNFAVAGSTALPSEVLAQKKISSRVTNSSLSKQLDWMSTHFNITCLFKENDCTK 165

Query: 64  KLKNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPG 123
           KLKNALFFVGEIGGNDYNYALFQGK+IEEV NMVPEVVQ IKEAV RVI+YGA +VIVPG
Sbjct: 166 KLKNALFFVGEIGGNDYNYALFQGKTIEEVRNMVPEVVQIIKEAVQRVINYGAFQVIVPG 225

Query: 124 NFPIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY 183
           NFPIGC+ IYL GF+T D  AYDELHCLK+LNN A YHN HL DAI+SLRK+NPNV IVY
Sbjct: 226 NFPIGCLPIYLAGFQTDDATAYDELHCLKDLNNLAIYHNTHLNDAIKSLRKENPNVNIVY 285

Query: 184 -----------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISW 243
                            GFDETSL K  CGM GDY+FNLMQMCG PGVPVC NP ERISW
Sbjct: 286 GDYYNALLWVLREASSLGFDETSLHKPCCGMDGDYEFNLMQMCGAPGVPVCGNPGERISW 345

Query: 244 DGVHLTQETYKYMTYWLIHDIFPKLHCI 254
           DG+HLTQ  Y+ MTYWLI +I P+L+CI
Sbjct: 346 DGIHLTQNAYRLMTYWLILNILPRLYCI 373

[Close]

BLAST of Lsi11G016150 vs. NCBI nr
Match: XP_022952109.1 (acetylajmalan esterase-like [Cucurbita moschata])

HSP 1 Score: 387.9 bits (995), Expect = 6.8e-104
Identity = 191/268 (71.27%), Postives = 213/268 (79.48%), Query Frame = 0


Query: 4   APMSQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTE 63
           APM+ GVNFAVAGST LPSE+LA+K I S VTNSSL+KQLDWM+THFN TC   + DCT+
Sbjct: 101 APMTHGVNFAVAGSTPLPSEVLAQKRISSRVTNSSLSKQLDWMSTHFNITCLFKENDCTK 160

Query: 64  KLKNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPG 123
           KLKNALFFVGEIGGNDYNYALFQGK+IEEV NMVPEVVQ IKEAV RVI+YGA +VIVPG
Sbjct: 161 KLKNALFFVGEIGGNDYNYALFQGKTIEEVRNMVPEVVQIIKEAVERVINYGAFQVIVPG 220

Query: 124 NFPIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY 183
           NFPIGC+ I+L GF+T D  AYDELHCLK+LNN A YHN HL DAI+S RK+NPNV IVY
Sbjct: 221 NFPIGCLPIFLAGFQTDDATAYDELHCLKDLNNLAIYHNTHLNDAIKSFRKENPNVNIVY 280

Query: 184 -----------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISW 243
                            GFDETSL K  CGM GDY+FNLMQMCG PGVPVC NP ERISW
Sbjct: 281 GDYYNALLWVLREASSLGFDETSLHKPCCGMDGDYEFNLMQMCGAPGVPVCGNPGERISW 340

Query: 244 DGVHLTQETYKYMTYWLIHDIFPKLHCI 254
           DG HLTQ  Y+ +TYWLI +I P+L+CI
Sbjct: 341 DGFHLTQNAYRLVTYWLILNILPRLYCI 368

[Close]

BLAST of Lsi11G016150 vs. NCBI nr
Match: XP_011659746.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 2.9e-102
Identity = 184/267 (68.91%), Postives = 212/267 (79.40%), Query Frame = 0


Query: 6   MSQGVNFAVAGSTALPSEILAEK-MIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKL 65
           M  GVNFAVAGSTALPS+ L+    I SPVTNSSL  QLDWM +HFN+ C +QR+C EKL
Sbjct: 104 MDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKL 163

Query: 66  KNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNF 125
           ++ALF VGEIGGNDYNYALFQGK+I+E ++MVP+VVQTIK AV +VISYGATRV+VPGNF
Sbjct: 164 RSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNF 223

Query: 126 PIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY-- 185
           PIGC  IYLTGF T D +AYDELHCLK+LN+FA YHN  +K AI  L+K+NP+ +IVY  
Sbjct: 224 PIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAVIVYGD 283

Query: 186 ---------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDG 245
                          GFDE SLQKS CG+GGDY FNLMQMCG+ GV  CPNP E ISWDG
Sbjct: 284 YYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDG 343

Query: 246 VHLTQETYKYMTYWLIHDIFPKLHCIV 255
           VHLTQ+TYK+MT+WLIHDIFPKLHCIV
Sbjct: 344 VHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of Lsi11G016150 vs. NCBI nr
Match: KAE8646130.1 (hypothetical protein Csa_016115 [Cucumis sativus])

HSP 1 Score: 382.5 bits (981), Expect = 2.9e-102
Identity = 184/267 (68.91%), Postives = 212/267 (79.40%), Query Frame = 0


Query: 6   MSQGVNFAVAGSTALPSEILAEK-MIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKL 65
           M  GVNFAVAGSTALPS+ L+    I SPVTNSSL  QLDWM +HFN+ C +QR+C EKL
Sbjct: 73  MDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNSICHNQRECNEKL 132

Query: 66  KNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNF 125
           ++ALF VGEIGGNDYNYALFQGK+I+E ++MVP+VVQTIK AV +VISYGATRV+VPGNF
Sbjct: 133 RSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNF 192

Query: 126 PIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY-- 185
           PIGC  IYLTGF T D +AYDELHCLK+LN+FA YHN  +K AI  L+K+NP+ +IVY  
Sbjct: 193 PIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLKKENPHAVIVYGD 252

Query: 186 ---------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDG 245
                          GFDE SLQKS CG+GGDY FNLMQMCG+ GV  CPNP E ISWDG
Sbjct: 253 YYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEACPNPNEHISWDG 312

Query: 246 VHLTQETYKYMTYWLIHDIFPKLHCIV 255
           VHLTQ+TYK+MT+WLIHDIFPKLHCIV
Sbjct: 313 VHLTQKTYKFMTHWLIHDIFPKLHCIV 339

[Close]

BLAST of Lsi11G016150 vs. NCBI nr
Match: XP_016903430.1 (PREDICTED: acetylajmalan esterase-like [Cucumis melo])

HSP 1 Score: 382.1 bits (980), Expect = 3.7e-102
Identity = 184/267 (68.91%), Postives = 212/267 (79.40%), Query Frame = 0


Query: 6   MSQGVNFAVAGSTALPSEILAEK-MIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKL 65
           M  GVNFAVAGSTAL S+ L+ K  I SPVTNSSL  QL+WM +HFN+ C +QRDC EKL
Sbjct: 104 MDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNSICHNQRDCNEKL 163

Query: 66  KNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNF 125
           ++ALFFVGEIGGNDYNYALFQGK+I+E ++MVP+VVQTIK AV +VISYGATRV+VPGNF
Sbjct: 164 RSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVISYGATRVVVPGNF 223

Query: 126 PIGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY-- 185
           PIGC  IYLTGF T D +AYDELHCLK+LN FA YHN  +K  I  L+K+NP+ +IVY  
Sbjct: 224 PIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLKKENPHTVIVYGD 283

Query: 186 ---------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDG 245
                          GFDE SLQKS CG+GGDY+FNLMQMCG+ GV  CPNP E ISWDG
Sbjct: 284 YYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEACPNPNEHISWDG 343

Query: 246 VHLTQETYKYMTYWLIHDIFPKLHCIV 255
           VHLTQ+TYK+MT+WLIHDIFPKLHCIV
Sbjct: 344 VHLTQKTYKFMTHWLIHDIFPKLHCIV 370

[Close]

BLAST of Lsi11G016150 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 241.1 bits (614), Expect = 9.6e-64
Identity = 124/258 (48.06%), Postives = 161/258 (62.40%), Query Frame = 0


Query: 10  VNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTEKLKNAL 69
           VNF V+GSTAL S   +E+ ++ P TN+ L+ QL W   H  +TC     DC   LK++L
Sbjct: 66  VNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSL 125

Query: 70  FFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIGC 129
           F VGEIGGNDYNY  FQGK +EE+ + +P VV  I  A   VI  GA  V+VPGNFP+GC
Sbjct: 126 FMVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGC 185

Query: 130 MSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYG----- 189
             IYLT F  KD   YD+  CL +LN FA  HN  L++AI SLRK+ P+V IVYG     
Sbjct: 186 FPIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNA 245

Query: 190 ---------FDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHLTQET 249
                    FD++   KS CG GG Y+++  +  G  GVPVC NP + ISWDGVHLTQ+ 
Sbjct: 246 FQYVLRSERFDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKA 305

Query: 250 YKYMTYWLIHDIFPKLHC 253
           Y++M+ +L + I  ++ C
Sbjct: 306 YRFMSKFLNNQILSQIKC 320

[Close]

BLAST of Lsi11G016150 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.2e-52
Identity = 107/258 (41.47%), Postives = 153/258 (59.30%), Query Frame = 0


Query: 4   APMSQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEK 63
           A   +GVNFAVAG+TAL    L E+ I+S +TN SL+ QL          C    DC + 
Sbjct: 107 ANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNLCGSPSDCRDM 166

Query: 64  LKNALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGN 123
           ++NAL  +GEIGGNDYN+ALFQ K ++EVE +VP V+ TI  A+T ++  G    +VPGN
Sbjct: 167 IENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGN 226

Query: 124 FPIGCMSIYLTGFKTKDYNAYDEL-HCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVY 183
           FPIG  + YLT +KT +   YD L  CLK LN+F+ Y+N  L++ +  LRK  P+V I+Y
Sbjct: 227 FPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIY 286

Query: 184 -----------------GFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISW 243
                            GF    L  + CG+GG Y+FN  + CG  GV  C +P + +++
Sbjct: 287 ADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNY 346

[Close]

BLAST of Lsi11G016150 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 192.2 bits (487), Expect = 5.1e-49
Identity = 103/248 (41.53%), Postives = 139/248 (56.05%), Query Frame = 0


Query: 8   QGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTCSHQRDCTEKLKNA 67
           +GVNFAVAG+TAL S  L ++ I  P TN SL  QL          C    DC + + NA
Sbjct: 106 KGVNFAVAGATALKSSFLKKRGI-QPHTNVSLGVQLKSFKKSLPNLCGSPSDCRDMIGNA 165

Query: 68  LFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPIG 127
           L  +GEIGGNDYN+  F  K ++EVE +VP V+ +I   +T +I  G    +VPG FPIG
Sbjct: 166 LILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIG 225

Query: 128 CMSIYLTGFKTKDYNAYD-ELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYGFDE 187
           C  +YLT +KT + + YD    CLK LN F  YH+  LK  +  LRK  P+V I+Y    
Sbjct: 226 CSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYY 285

Query: 188 TSLQK----------------SGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVHL 239
            SL +                + CG+GG Y+FN  + CG  GV  C +P + + WDGVH+
Sbjct: 286 NSLLRIFKEPAKFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHM 345

[Close]

BLAST of Lsi11G016150 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 8.7e-49
Identity = 104/256 (40.62%), Postives = 144/256 (56.25%), Query Frame = 0


Query: 7   SQGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTEKLK 66
           +QG+NFAV G+TAL    L ++ I S  TN SL+ QL+         C S  RDC E L 
Sbjct: 111 NQGINFAVYGATALDRAFLVKQGIKSDFTNISLSVQLNTFKQILPNLCASSTRDCREMLG 170

Query: 67  NALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFP 126
           ++L  +GEIGGNDYNY  F+GKSI E++ +VP +++ I  A+  +I  G    +VPGNFP
Sbjct: 171 DSLILMGEIGGNDYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFP 230

Query: 127 IGCMSIYLTGFKTKDYNAYDELHCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIV---- 186
           IGC + YLT F+T          C+  LN F  +HN  LK  ++ L+K  P+V I+    
Sbjct: 231 IGCSTAYLTLFQTATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADY 290

Query: 187 -------------YGFDETSLQKSGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGV 245
                        YGF    L  + CG+GG Y+F + + CG  GV  C NP E ++WDG 
Sbjct: 291 YNSLYGLFQEPAKYGFKNRPL-AACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGY 350

[Close]

BLAST of Lsi11G016150 vs. TAIR 10
Match: AT1G28670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 190.3 bits (482), Expect = 1.9e-48
Identity = 105/255 (41.18%), Postives = 141/255 (55.29%), Query Frame = 0


Query: 8   QGVNFAVAGSTALPSEILAEKMIYSPVTNSSLTKQLDWMATHFNTTC-SHQRDCTEKLKN 67
           QG+NFAV G+TAL    L  K I S  TN SL+ QLD         C S  RDC E L +
Sbjct: 110 QGINFAVYGATALDRAFLLGKGIESDFTNVSLSVQLDTFKQILPNLCASSTRDCKEMLGD 169

Query: 68  ALFFVGEIGGNDYNYALFQGKSIEEVENMVPEVVQTIKEAVTRVISYGATRVIVPGNFPI 127
           +L  +GEIGGNDYNY  F+GKSI E++ +VP +V+ I  A+  +I  G    +VPG FP 
Sbjct: 170 SLILMGEIGGNDYNYPFFEGKSINEIKELVPLIVKAISSAIVDLIDLGGKTFLVPGGFPT 229

Query: 128 GCMSIYLTGFKTKDYNAYDEL-HCLKNLNNFARYHNYHLKDAIRSLRKDNPNVMIVYGFD 187
           GC + YLT F+T      D L  C   LN F  +HN  LK  ++ L+K  P+V I+Y   
Sbjct: 230 GCSAAYLTLFQTVAEKDQDPLTGCYPLLNEFGEHHNEQLKTELKRLQKFYPHVNIIYADY 289

Query: 188 ETSLQK----------------SGCGMGGDYDFNLMQMCGLPGVPVCPNPEERISWDGVH 245
             SL +                + CG+GG Y+F + + CG  GV  C NP E ++WDG H
Sbjct: 290 HNSLYRFYQEPAKYGFKNKPLAACCGVGGKYNFTIGKECGYEGVNYCQNPSEYVNWDGYH 349

The following BLAST results are available for this feature:

BLAST of Lsi11G016150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZB2	1.3e-62	48.06	GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2...	[more]
Q8RXT9	3.1e-51	41.47	GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2...	[more]
Q3MKY2	3.8e-49	40.23	Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1	[more]
Q3ZFI4	5.5e-48	40.78	Sinapine esterase OS=Brassica napus OX=3708 PE=1 SV=1	[more]
Q94F40	7.2e-48	41.53	GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2...	[more]

BLAST of Lsi11G016150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GKN7	3.3e-104	71.27	acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111454872 PE=3 S...	[more]
A0A1S4E629	1.8e-102	68.91	acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1	[more]
A0A6J1KFS3	4.0e-102	67.68	acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=...	[more]
A0A5D3CNR6	6.9e-102	68.54	Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A6J1K9I7	1.2e-101	67.30	acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493500 PE=3 SV=...	[more]

BLAST of Lsi11G016150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023553636.1	2.5e-106	72.76	LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like [Cucurbita pepo subsp. ...	[more]
XP_022952109.1	6.8e-104	71.27	acetylajmalan esterase-like [Cucurbita moschata]	[more]
XP_011659746.1	2.9e-102	68.91	acetylajmalan esterase-like [Cucumis sativus]	[more]
KAE8646130.1	2.9e-102	68.91	hypothetical protein Csa_016115 [Cucumis sativus]	[more]
XP_016903430.1	3.7e-102	68.91	PREDICTED: acetylajmalan esterase-like [Cucumis melo]	[more]

BLAST of Lsi11G016150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G03980.1	9.6e-64	48.06	SGNH hydrolase-type esterase superfamily protein	[more]
AT1G28590.1	2.2e-52	41.47	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28600.1	5.1e-49	41.53	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28650.1	8.7e-49	40.63	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G28670.1	1.9e-48	41.18	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 23..240 e-value: 5.3E-18 score: 66.3
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 2..246 e-value: 1.5E-39 score: 138.3
None	No IPR available	PANTHER	PTHR22835:SF581	ACETYLAJMALAN ESTERASE-LIKE	coord: 8..252
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 8..252
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 60..243

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G016150.1	Lsi11G016150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds