Lsi11G015260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G015260
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionLipoyl synthase, mitochondrial
Locationchr11: 23667800 .. 23670100 (+)
RNA-Seq ExpressionLsi11G015260
SyntenyLsi11G015260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCAAGTCAAGCCCACTTCACTTTTACATTGGGCCACAAATATTAATCATCAAGCCCAATACTACCTTTACGTATGGCTGAAAACGCCTTATGAACGAACGCCCTTCAACTGTCCAAATCCGAAGCCACCCAAATTCCGGTCAGGCTTCTCTTCGAGATCGCCGACCACGATGTACAAACGCTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCCTTCTCCTCTTCATCTTCATCCACCTCCGCCTCTGCTACCTCCTCCCAATTCCCCCGCACTTTGGCCGGTCTCCGGGAACGACTCGCCGCCGAGTCCCCTTCACTTTCCGACTTCGTTGACCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGCTGCAGGTTAGTAGTAATTCAGTTTATTAATTGTCAATGTGTTTTTGACGACTGAGGAGAGAAGAACCGTAGTTTCTGGTTATTTTCCTGTGTATAATGTCAACGTATATATAATATAGTGGGGAGCGTGAATGGATTTAGAACTGATTGTTTTTGTCATAGAAATAACTGTGTTGATTACTTTGATATCATCTCGGTGCCTCAGGATTTCTGTTAATTACATTGCTGTTTTGGGATTGTTCGAAATCCAGGTTCTGTAACGTGAAAACTTCACGAACTCCACCTCCACCGGACCCAAATGAGCCGACAAATGTTGCCGAGGCAATCGCATCATGGGGTTTGGATTATGTGGTCATCACTAGTGTTGATCGCGACGATTTGCCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCGAATATGCTGATTGAGGCATTGGGTACAAATTCCCTAGTTTCTATTACGCATAACAGTGTCACGTTCATTGTTGCATTGAAGGATCCTTGATATGACGCTTGATTTTAAAAAGCCTTATTTTATTTGGTTTCGATCCAACTAAGGAATTTGGTGAAACCTACTTATACCCTGTACTATTGATGTCGCAAAAATCAGCATCATATGCTGATTTGCTTCTGAATATGTTGTCCAGTGTGATTTAATCTGTTCTTTGATGTTGCTAATGGTTAACTAATTGATTCTGCAGTTCCTGATTTTCGAGGAGACCCTGGCTGTGTAGAGAAAGTTGCAAAGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAGGGGGCAGTTAGGGATCACCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCTCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTATATGAGACCTTCAAAGCGCCATATGCCTGTATTGGAATACATTACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGTACTCTCTCTTTCTTATTATTTGAACATTTTGATTGTGTACCGTCTTCTTTGTTCTGCTAGGGATTGATTAATCAAGTCCTTAAAGGCTAAAGCCCACTTGATTTTTCTTTTTTTAAATGTCTGGTATTGTATCATTTCCCTCGTATTTTCTTGGACGGATCGACTTATTAAGTGCTTAATTTCTTTTTCAATTAAAAAACTTTGTCAAGCACACTTAAAAAGTCAATCCAAACAGATCCTATTGGATTTATACCGTTGTAATTTTATGTGGGTTTTAATTGTTCTAGGGAGTCCATGTTTTACTTTAAAGCATATACTAGTTCACACATGCATAACCCTCATTTAGTTTATAGGAAATCCTCACCATCATGTGTCACATGATCAATAGTTTGTTAATGGATGGAGTTACTTGCCATGCCTTGAGGGTGCAACAATTATCTAAAAATCCATTTTTTGTTTTGTTGTGGCAGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACCTTGA

mRNA sequence

ATGGATCAAGTCAAGCCCACTTCACTTTTACATTGGGCCACAAATATTAATCATCAAGCCCAATACTACCTTTACGTATGGCTGAAAACGCCTTATGAACGAACGCCCTTCAACTGTCCAAATCCGAAGCCACCCAAATTCCGGTCAGGCTTCTCTTCGAGATCGCCGACCACGATGTACAAACGCTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCCTTCTCCTCTTCATCTTCATCCACCTCCGCCTCTGCTACCTCCTCCCAATTCCCCCGCACTTTGGCCGGTCTCCGGGAACGACTCGCCGCCGAGTCCCCTTCACTTTCCGACTTCGTTGACCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGCTGCAGGTTCTGTAACGTGAAAACTTCACGAACTCCACCTCCACCGGACCCAAATGAGCCGACAAATGTTGCCGAGGCAATCGCATCATGGGGTTTGGATTATGTGGTCATCACTAGTGTTGATCGCGACGATTTGCCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCGAATATGCTGATTGAGGCATTGGTTCCTGATTTTCGAGGAGACCCTGGCTGTGTAGAGAAAGTTGCAAAGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAGGGGGCAGTTAGGGATCACCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCTCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTATATGAGACCTTCAAAGCGCCATATGCCTGTATTGGAATACATTACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACCTTGA

Coding sequence (CDS)

ATGGATCAAGTCAAGCCCACTTCACTTTTACATTGGGCCACAAATATTAATCATCAAGCCCAATACTACCTTTACGTATGGCTGAAAACGCCTTATGAACGAACGCCCTTCAACTGTCCAAATCCGAAGCCACCCAAATTCCGGTCAGGCTTCTCTTCGAGATCGCCGACCACGATGTACAAACGCTGCCAAAGCCTTGCTCGAATCCTCAAATCCGTCAGTAGATCCTTCTCCTCTTCATCTTCATCCACCTCCGCCTCTGCTACCTCCTCCCAATTCCCCCGCACTTTGGCCGGTCTCCGGGAACGACTCGCCGCCGAGTCCCCTTCACTTTCCGACTTCGTTGACCTGCAATCTTCTGATTCGTATTCTGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCTGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAGCTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTAGGGGAATGCTGGTCTGGTGGAGAAACCGGCACTGCCACTGCTACGATTATGATTCTTGGTGACACTTGTACCCGTGGCTGCAGGTTCTGTAACGTGAAAACTTCACGAACTCCACCTCCACCGGACCCAAATGAGCCGACAAATGTTGCCGAGGCAATCGCATCATGGGGTTTGGATTATGTGGTCATCACTAGTGTTGATCGCGACGATTTGCCTGATCAAGGGAGTGGTCATTTTGCAGAGACTGTGCAGAAACTTAAGGTGTTGAAGCCGAATATGCTGATTGAGGCATTGGTTCCTGATTTTCGAGGAGACCCTGGCTGTGTAGAGAAAGTTGCAAAGTCAGGCCTTGACGTCTTTGCTCACAATATAGAGACAGTTGAAGAGCTTCAGGGGGCAGTTAGGGATCACCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCCAAAGAGTTTGCTCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTTGTGCAAACAATGGAGAAGGTGAGAGCAGCTGGAGTTGATGTGATGACGTTTGGTCAGTATATGAGACCTTCAAAGCGCCATATGCCTGTATTGGAATACATTACTCCTGAGGCTTTCGAGAAGTATCAAAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACAGCCTACCCAACCTTGA

Protein sequence

MDQVKPTSLLHWATNINHQAQYYLYVWLKTPYERTPFNCPNPKPPKFRSGFSSRSPTTMYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQP
Homology
[Close]
BLAST of Lsi11G015260 vs. ExPASy Swiss-Prot
Match: Q3LSN5 (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.2e-183
Identity = 318/375 (84.80%), Postives = 348/375 (92.80%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           MY R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHF ETVQKLK LKP+MLIEALVPDFRG+  CVEKV+KSGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQ+MRPSKRHMPV EYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 419 FYIKSMIESDRAASN 434
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

[Close]
BLAST of Lsi11G015260 vs. ExPASy Swiss-Prot
Match: Q3LSN4 (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.6e-183
Identity = 318/375 (84.80%), Postives = 347/375 (92.53%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           MY R +++A  L   ++  SSSS++T+ ++  S+  + LA LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHF ETVQKLK LKP+ LIEALVPDFRG+  CVEKV+KSGLDVFAHNI
Sbjct: 182 SVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 419 FYIKSMIESDRAASN 434
           FYIKSMI+SDRAAS+
Sbjct: 362 FYIKSMIDSDRAASS 376

[Close]
BLAST of Lsi11G015260 vs. ExPASy Swiss-Prot
Match: B9H5L9 (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 638.6 bits (1646), Expect = 5.0e-182
Identity = 317/366 (86.61%), Postives = 342/366 (93.44%), Query Frame = 0 

Query: 71  KSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTK 130
           K+ +R+FSSS+   S++    QF +TLAGLR RLA ESP+LSDF+ LQS+++YSVEVGTK
Sbjct: 19  KAKNRTFSSSTVE-SSTKQPPQFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTK 78

Query: 131 KKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 190
           KKPLPKPKWM+E++PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI
Sbjct: 79  KKPLPKPKWMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATI 138

Query: 191 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGS 250
           MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDL DQGS
Sbjct: 139 MILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLADQGS 198

Query: 251 GHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRD 310
           GHFAETV KLK LKPNMLIEALVPDFRGD GCVEKVAKSGLDVFAHNIETVEELQ +VRD
Sbjct: 199 GHFAETVHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVEELQSSVRD 258

Query: 311 HRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQY 370
           HRANFKQSLDVL+MAKE+AP GTLTKTSIMLGCGE P+QVV+TMEKVRAAGVDVMTFGQY
Sbjct: 259 HRANFKQSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGVDVMTFGQY 318

Query: 371 MRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRA 430
           MRPSKRHMPV EYITP+AFEKY+ LGM+MGFRYVASGPMVRSSYKAGEFYIKSMIESDR+
Sbjct: 319 MRPSKRHMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIKSMIESDRS 378

Query: 431 ASNSQP 437
            S+  P
Sbjct: 379 VSSQLP 383

[Close]
BLAST of Lsi11G015260 vs. ExPASy Swiss-Prot
Match: B7FM45 (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 1.5e-181
Identity = 312/375 (83.20%), Postives = 348/375 (92.80%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           MY R +++A+ LKS ++ FS ++++T+ + +SS+FP+ L  LR RLA ESPSLSDF+ L+
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           S+++YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEAKCPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDP+EPTNVAEAIASWGLDYVVIT
Sbjct: 122 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPDEPTNVAEAIASWGLDYVVIT 181

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SV RDDLPDQGS HF ETVQKLK+LKP++LIEALVPDFRG+  CVEKV+KSGLDVFAHNI
Sbjct: 182 SVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAECVEKVSKSGLDVFAHNI 241

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQ AVRDHRANF QSLDVL MAK++AP+GTLTKTSIMLGCGETPDQ+V+TMEKVR
Sbjct: 242 ETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLGCGETPDQIVKTMEKVR 301

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 302 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFRYVASGPMVRSSYKAGE 361

Query: 419 FYIKSMIESDRAASN 434
           FYIKSMI+SDRA S+
Sbjct: 362 FYIKSMIDSDRAVSS 376

[Close]
BLAST of Lsi11G015260 vs. ExPASy Swiss-Prot
Match: A5CB81 (Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 5.7e-178
Identity = 306/348 (87.93%), Postives = 327/348 (93.97%), Query Frame = 0 

Query: 85  SASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESV 144
           S+  T+ + P+TLA LR RLA ESPSLSDFV LQ+SD YSVEVGTKKKPL KPKWMKES+
Sbjct: 34  SSPPTNPEPPQTLASLRHRLAVESPSLSDFVRLQTSDDYSVEVGTKKKPLSKPKWMKESI 93

Query: 145 PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRFC 204
           PGG KY QIKKKLR+L LHTVCEEA+CPN+GECWSGGETGTATATIMILGDTCTRGCRFC
Sbjct: 94  PGGAKYTQIKKKLRQLNLHTVCEEARCPNMGECWSGGETGTATATIMILGDTCTRGCRFC 153

Query: 205 NVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLK 264
           NVKTSRTPPPPDP+EP+ VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLK+LK
Sbjct: 154 NVKTSRTPPPPDPDEPSKVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKILK 213

Query: 265 PNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVM 324
           PNMLIEALVPDFRGDPGCVEKV+KSGLDVFAHNIETVEELQ AVRDHRANFKQSL+VL +
Sbjct: 214 PNMLIEALVPDFRGDPGCVEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLEVLKL 273

Query: 325 AKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVLEYI 384
           AKE+A +GTLTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPV EYI
Sbjct: 274 AKEYADAGTLTKTSIMLGCGETPDQVVRTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYI 333

Query: 385 TPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAAS 433
           TPEAFEKY+ LGM MGFRYVASGPMVRSSYKAGEFYIKSMI++DRA S
Sbjct: 334 TPEAFEKYRILGMDMGFRYVASGPMVRSSYKAGEFYIKSMIDADRAMS 381

[Close]
BLAST of Lsi11G015260 vs. ExPASy TrEMBL
Match: A0A5A7SKJ1 (Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.0e-210
Identity = 375/380 (98.68%), Postives = 377/380 (99.21%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M KRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQ 439
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

[Close]
BLAST of Lsi11G015260 vs. ExPASy TrEMBL
Match: A0A1S4E162 (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 741.5 bits (1913), Expect = 2.0e-210
Identity = 375/380 (98.68%), Postives = 377/380 (99.21%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M KRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQ 439
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

[Close]
BLAST of Lsi11G015260 vs. ExPASy TrEMBL
Match: A0A0A0K1R3 (Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 1.6e-207
Identity = 370/380 (97.37%), Postives = 374/380 (98.42%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M KRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQG VRDHRANFKQSLDVLVMAKEF+ SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQ 439
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

[Close]
BLAST of Lsi11G015260 vs. ExPASy TrEMBL
Match: A0A6J1D3N5 (Lipoyl synthase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016734 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 9.1e-203
Identity = 362/376 (96.28%), Postives = 365/376 (97.07%), Query Frame = 0 

Query: 63  CQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDS 122
           CQSL RIL+S  RSFSSSSSSTSASATSSQFPRTLA LRERLAAESPSLSDFV LQS+DS
Sbjct: 5   CQSLVRILESFGRSFSSSSSSTSASATSSQFPRTLAALRERLAAESPSLSDFVGLQSTDS 64

Query: 123 YSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGE 182
           YSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGE
Sbjct: 65  YSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGE 124

Query: 183 TGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDR 242
           TGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVITSVDR
Sbjct: 125 TGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDR 184

Query: 243 DDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVE 302
           DDLPDQGSGHFAETVQ LKVLKPNMLIEALVPDFRGD GCVEKVAKSGLDVFAHNIETVE
Sbjct: 185 DDLPDQGSGHFAETVQTLKVLKPNMLIEALVPDFRGDIGCVEKVAKSGLDVFAHNIETVE 244

Query: 303 ELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGV 362
           ELQGAVRDHRANFKQSLDVL MAK+FAPSGTLTKTSIMLGCGE PDQVVQTMEKVRAAGV
Sbjct: 245 ELQGAVRDHRANFKQSLDVLAMAKDFAPSGTLTKTSIMLGCGEKPDQVVQTMEKVRAAGV 304

Query: 363 DVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIK 422
           DVMTFGQYMRPSKRHMPV EYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIK
Sbjct: 305 DVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIK 364

Query: 423 SMIESDRAASNSQPTQ 439
           SMIESDRAASNSQPTQ
Sbjct: 365 SMIESDRAASNSQPTQ 380

[Close]
BLAST of Lsi11G015260 vs. ExPASy TrEMBL
Match: A0A6J1JNE9 (Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111486046 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.8e-201
Identity = 361/381 (94.75%), Postives = 365/381 (95.80%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M  R  SLARI +S+ RS S SSSSTSASATSSQ PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPG VEKVAKSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKY NLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQP 440
           FYIKSMIESDRAASNS PTQP
Sbjct: 361 FYIKSMIESDRAASNSHPTQP 381

[Close]
BLAST of Lsi11G015260 vs. NCBI nr
Match: XP_038887854.1 (lipoyl synthase 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 741.5 bits (1913), Expect = 4.2e-210
Identity = 375/381 (98.43%), Postives = 376/381 (98.69%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M KRCQSL RILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLTRILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGD GCVEKVAKSGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVHKLKVLKPNMLIEALVPDFRGDTGCVEKVAKSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQP 440
           FYIKSMIESDRAASNSQPT+P
Sbjct: 361 FYIKSMIESDRAASNSQPTRP 381

[Close]
BLAST of Lsi11G015260 vs. NCBI nr
Match: XP_016901725.1 (PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo] >KAA0031592.1 lipoyl synthase 2 [Cucumis melo var. makuwa] >TYK07044.1 lipoyl synthase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 741.5 bits (1913), Expect = 4.2e-210
Identity = 375/380 (98.68%), Postives = 377/380 (99.21%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M KRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQ 439
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

[Close]
BLAST of Lsi11G015260 vs. NCBI nr
Match: XP_004136815.1 (lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical protein Csa_020197 [Cucumis sativus])

HSP 1 Score: 731.9 bits (1888), Expect = 3.3e-207
Identity = 370/380 (97.37%), Postives = 374/380 (98.42%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M KRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESP LSDFVDLQ
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPILSDFVDLQ 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
           SSDSYSVEVGTKKKPLPKPKWM+ESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMRESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGD GCVEKVA+SGLDVFAHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGCVEKVARSGLDVFAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQG VRDHRANFKQSLDVLVMAKEF+ SGTLTKTSIMLGCGETPDQVVQTMEKVR
Sbjct: 241 ETVEELQGVVRDHRANFKQSLDVLVMAKEFSLSGTLTKTSIMLGCGETPDQVVQTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EYITPEAFEKYQN+GMKMGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNIGMKMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNSQPTQ 439
           FYIKSMIESDRAASNSQPTQ
Sbjct: 361 FYIKSMIESDRAASNSQPTQ 380

[Close]
BLAST of Lsi11G015260 vs. NCBI nr
Match: KAG6572348.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 718.8 bits (1854), Expect = 2.9e-203
Identity = 370/413 (89.59%), Postives = 384/413 (92.98%), Query Frame = 0 

Query: 29  KTPYERTPFNCPNPKPPKFRSGFSSRSPTTMYKRCQSLARILKSVSRSFSSSSSSTSASA 88
           KT Y+RT F C     P+   G ++    TM    +SLAR+L+SV RSFSSSSSST+ASA
Sbjct: 6   KTLYQRTSFIC-----PELLYGVAN---LTMNSHGRSLARLLESVGRSFSSSSSSTAASA 65

Query: 89  TSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGE 148
           TSSQF RTLAGLRERLA E+PSLSDF+DLQSS+SYSVEVGTKKKPLPKPKWMKESVPGGE
Sbjct: 66  TSSQFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGE 125

Query: 149 KYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCR--FCNV 208
           KYVQIKKKLRELKLHTVCEEA+CPNLGECWSGGETGTATATIMILGDTCTRGCR  FCNV
Sbjct: 126 KYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLVFCNV 185

Query: 209 KTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPN 268
           KTSRTPPPPDPNEP NVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPN
Sbjct: 186 KTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPN 245

Query: 269 MLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAK 328
           MLIEALVPDFRGDPGCVE VAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAK
Sbjct: 246 MLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAK 305

Query: 329 EFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVLEYITP 388
           EFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPV EYITP
Sbjct: 306 EFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITP 365

Query: 389 EAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQP 440
           EAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDR+AS+S PTQP
Sbjct: 366 EAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 410

[Close]
BLAST of Lsi11G015260 vs. NCBI nr
Match: KAG7011959.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 718.4 bits (1853), Expect = 3.8e-203
Identity = 370/414 (89.37%), Postives = 384/414 (92.75%), Query Frame = 0 

Query: 29  KTPYERTPFNCPNPKPPKFRSGFSSRSPTTMYKRCQSLARILKSVSRSFSSSSSSTSASA 88
           KT Y+RT F C     P+   G ++    TM    +SLAR+L+SV RSFSSSSSST+ASA
Sbjct: 6   KTLYQRTSFIC-----PELLYGVAN---LTMNSHGRSLARLLESVGRSFSSSSSSTAASA 65

Query: 89  TSSQFPRTLAGLRERLAAESPSLSDFVDLQSSDSYSVEVGTKKKPLPKPKWMKESVPGGE 148
           TSSQF RTLAGLRERLA E+PSLSDF+DLQSS+SYSVEVGTKKKPLPKPKWMKESVPGGE
Sbjct: 66  TSSQFSRTLAGLRERLAVETPSLSDFLDLQSSESYSVEVGTKKKPLPKPKWMKESVPGGE 125

Query: 149 KYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGC---RFCN 208
           KYVQIKKKLRELKLHTVCEEA+CPNLGECWSGGETGTATATIMILGDTCTRGC   RFCN
Sbjct: 126 KYVQIKKKLRELKLHTVCEEARCPNLGECWSGGETGTATATIMILGDTCTRGCRLSRFCN 185

Query: 209 VKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKP 268
           VKTSRTPPPPDPNEP NVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKP
Sbjct: 186 VKTSRTPPPPDPNEPKNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKP 245

Query: 269 NMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMA 328
           NMLIEALVPDFRGDPGCVE VAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMA
Sbjct: 246 NMLIEALVPDFRGDPGCVENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMA 305

Query: 329 KEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVLEYIT 388
           KEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPV EYIT
Sbjct: 306 KEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYIT 365

Query: 389 PEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQP 440
           PEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDR+AS+S PTQP
Sbjct: 366 PEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 411

[Close]
BLAST of Lsi11G015260 vs. TAIR 10
Match: AT2G20860.1 (lipoic acid synthase 1 )

HSP 1 Score: 624.8 bits (1610), Expect = 5.3e-179
Identity = 313/376 (83.24%), Postives = 339/376 (90.16%), Query Frame = 0 

Query: 59  MYKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 118
           M+ R   L R L+  SR FSSSS+ T  + T S  P++L  LR RLA ESPSL+DF+   
Sbjct: 1   MHSRSALLYRFLRPASRCFSSSSAVTPVTVTQS--PKSLEALRARLANESPSLTDFI--- 60

Query: 119 SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 178
             D+YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 179 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVIT 238
           SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEP NVAEAIASWG+DYVVIT
Sbjct: 121 SGGETGTATATIMILGDTCTRGCRFCNVKTSRTPPPPDPNEPNNVAEAIASWGVDYVVIT 180

Query: 239 SVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNI 298
           SVDRDDLPDQGSGHFAETVQ+LK LKP MLIEALVPDFRGD GCVEKV+KSGLDV AHNI
Sbjct: 181 SVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGCVEKVSKSGLDVLAHNI 240

Query: 299 ETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVR 358
           ETVEELQ  VRDHRANFKQSLDVL MAKE+AP+GTLTKTS+MLGCGETPDQVV+TMEKVR
Sbjct: 241 ETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVR 300

Query: 359 AAGVDVMTFGQYMRPSKRHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGE 418
           AAGVDVMTFGQYMRPSKRHMPV EY+TP+AFE+Y+ LGM+MGFRYVASGPMVRSSYKAGE
Sbjct: 301 AAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYKAGE 360

Query: 419 FYIKSMIESDRAASNS 435
           +YIKSMIE+DR AS S
Sbjct: 361 YYIKSMIEADRVASPS 371

[Close]
BLAST of Lsi11G015260 vs. TAIR 10
Match: AT5G08415.1 (Radical SAM superfamily protein )

HSP 1 Score: 379.4 bits (973), Expect = 3.9e-105
Identity = 176/299 (58.86%), Postives = 231/299 (77.26%), Query Frame = 0 

Query: 136 KPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGD 195
           KP W+++  P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGD
Sbjct: 97  KPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGD 156

Query: 196 TCTRGCRFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAE 255
           TCTRGCRFC VKTSR PPPPDP EP N A+AIASWG+DY+VITSVDRDD+PD GSGHFA+
Sbjct: 157 TCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHFAQ 216

Query: 256 TVQKLKVLKPNMLIEALVPDFRGDPGCVEKVAKSGLDVFAHNIETVEELQGAVRDHRANF 315
           TV+ +K  KP+++IE L  DFRGD   V+ +  SGLDVFAHN+ETV+ LQ  VRD RA +
Sbjct: 217 TVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGY 276

Query: 316 KQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSK 375
           +QS+ VL  AK   P G +TKTSIMLG GET +++ + M  +RA  VD++T GQY++P+ 
Sbjct: 277 EQSMSVLKHAKISKP-GMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQPTP 336

Query: 376 RHMPVLEYITPEAFEKYQNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNS 435
            H+ V EY+TPE F+ ++  G  +GFRYVASGP+VRSSY+AGE ++K+M++   + S S
Sbjct: 337 LHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKSLS 394

The following BLAST results are available for this feature:
BLAST of Lsi11G015260 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q3LSN51.2e-18384.80Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1[more]
Q3LSN41.6e-18384.80Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1[more]
B9H5L95.0e-18286.61Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1[more]
B7FM451.5e-18183.20Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1[more]
A5CB815.7e-17887.93Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1[more]
BLAST of Lsi11G015260 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7SKJ12.0e-21098.68Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE... [more]
A0A1S4E1622.0e-21098.68Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1[more]
A0A0A0K1R31.6e-20797.37Lipoyl synthase, mitochondrial OS=Cucumis sativus OX=3659 GN=LIP1 PE=3 SV=1[more]
A0A6J1D3N59.1e-20396.28Lipoyl synthase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016734 PE... [more]
A0A6J1JNE93.8e-20194.75Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111486046 PE=3 ... [more]
BLAST of Lsi11G015260 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038887854.14.2e-21098.43lipoyl synthase 2, mitochondrial [Benincasa hispida][more]
XP_016901725.14.2e-21098.68PREDICTED: lipoyl synthase 2, mitochondrial isoform X1 [Cucumis melo] >KAA003159... [more]
XP_004136815.13.3e-20797.37lipoyl synthase 2, mitochondrial [Cucumis sativus] >KGN43590.1 hypothetical prot... [more]
KAG6572348.12.9e-20389.59Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7011959.13.8e-20389.37Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
BLAST of Lsi11G015260 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G20860.15.3e-17983.24lipoic acid synthase 1 [more]
AT5G08415.13.9e-10558.86Radical SAM superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006638Elp3/MiaB/NifBSMARTSM00729MiaBcoord: 187..394
e-value: 3.2E-8
score: 43.3
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 180..402
e-value: 6.9E-14
score: 53.6
IPR003698Lipoyl synthaseTIGRFAMTIGR00510TIGR00510coord: 130..424
e-value: 4.7E-121
score: 401.7
IPR003698Lipoyl synthaseSFLDSFLDF00271lipoyl_synthasecoord: 128..424
e-value: 0.0
score: 433.8
IPR003698Lipoyl synthasePANTHERPTHR10949LIPOYL SYNTHASEcoord: 92..433
IPR003698Lipoyl synthaseHAMAPMF_00206Lipoyl_synthcoord: 136..430
score: 133.504227
IPR031691Lipoyl synthase, N-terminalPFAMPF16881LIAS_Ncoord: 63..171
e-value: 2.1E-17
score: 63.5
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 194..356
e-value: 7.8E-12
score: 45.9
IPR007197Radical SAMSFLDSFLDS00029Radical_SAMcoord: 128..424
e-value: 0.0
score: 433.8
IPR007197Radical SAMPROSITEPS51918RADICAL_SAMcoord: 182..402
score: 27.566725
NoneNo IPR availablePANTHERPTHR10949:SF0LIPOYL SYNTHASE, MITOCHONDRIALcoord: 92..433
NoneNo IPR availableCDDcd01335Radical_SAMcoord: 191..371
e-value: 1.42837E-7
score: 49.6394
NoneNo IPR availableSUPERFAMILY102114Radical SAM enzymescoord: 152..427
IPR027527Lipoyl synthase, mitochondrialHAMAPMF_03128Lipoyl_synth_plantMcoord: 78..433
score: 62.254391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G015260.1Lsi11G015260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009107 lipoate biosynthetic process
biological_process GO:0009249 protein lipoylation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016992 lipoate synthase activity
molecular_function GO:0102552 lipoyl synthase activity (acting on glycine-cleavage complex H protein
molecular_function GO:0102553 lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding