Lsi11G012940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G012940
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionHomoserine kinase
Locationchr11: 21587330 .. 21588451 (+)
RNA-Seq ExpressionLsi11G012940
SyntenyLsi11G012940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATGATCTCCTATCAACCGCCATTGAAGTCGTTGGCGATTCCTCCAGTTTCTTTATCTAACCCTAAATCTGTCCTCTTCAGGTGCAGTTTGTCTCTTCCATCCAGAACCGCCGTCACTTCCGTTGAACCTGAACCAGTATTCTCCTCCGTGAAGGCATTCGCACCTGCAACCGTCGCTAATTTAGGCCCTGGCTTCGATTTCCTTGGCTGTGCCGTCGATGGCTTGGGAGATTATGTCTCTCTCAGCGTTGACTCCAATGTTCATCCAGGTGAAGTTGCAATTACCAATATTACAGGAAATAACACTAATAAACTTAGTAAAAATCCTCTGTATAATTGCGCTGGAATTGCTGCCATTGAGGTTATGAAAATGTTAGGGATTCGATCTGTCGGTCTCTCTCTTTCGCTTGAGAAAGGTTTGCCGTTGGGGAGTGGATTGGGATCTAGTGCAGCGAGTGCAGCCGCTGCGGCGATTGCTGTTAATGGATTGTTCGGTGGAAAATTAGGAGTCGAGGAATTAGTTCTCGCCGGGTTAAAATCGGAAGAGAAGGTTTCTGGATACCATGCGGACAATGTCGCACCGGCTATTATGGGGGGATTCATTCTGATTAGGAATTACGAACCCTTGGAATTGATCCGCTTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTCAGCCCAGAATTCGAAGCTCCGACGAAGAAAATGCGGGCTGCTTTGCCGGCTGAAGTTGGGATGCCGCACCATGTATGGAATTCCAGCCAAGCCGGGGCGTTGGTAGCTGCAGTGCTGCAGGGCGACGCAATGGGATTGGGGAAAGCATTGTCGTCAGACAAAATCGTGGAGCCAAGGCGGTCGCCATTGATTCCAGGGATGGATGGGGTTAAGAAGGCAGCCATTGCTGCCGGGGCATTTGGGTGTACAATAAGCGGGGCGGGGCCAACGGCGGTGGCGGTAACCGACAACGAAGAGAAGGGGAAGGAGATTGGTGAGAGGATGGTTATGGCGTTTATGAAGGAAGGGAATTTGAAAGCTGAGGCATCTGTAAAGAGACTGGATCGAGTTGGTGCAAGGCTTATTGGATCAACTCCATTAGATAGAGTTTTATGA

mRNA sequence

ATGGCTATGATCTCCTATCAACCGCCATTGAAGTCGTTGGCGATTCCTCCAGTTTCTTTATCTAACCCTAAATCTGTCCTCTTCAGGTGCAGTTTGTCTCTTCCATCCAGAACCGCCGTCACTTCCGTTGAACCTGAACCAGTATTCTCCTCCGTGAAGGCATTCGCACCTGCAACCGTCGCTAATTTAGGCCCTGGCTTCGATTTCCTTGGCTGTGCCGTCGATGGCTTGGGAGATTATGTCTCTCTCAGCGTTGACTCCAATGTTCATCCAGGTGAAGTTGCAATTACCAATATTACAGGAAATAACACTAATAAACTTAGTAAAAATCCTCTGTATAATTGCGCTGGAATTGCTGCCATTGAGGTTATGAAAATGTTAGGGATTCGATCTGTCGGTCTCTCTCTTTCGCTTGAGAAAGGTTTGCCGTTGGGGAGTGGATTGGGATCTAGTGCAGCGAGTGCAGCCGCTGCGGCGATTGCTGTTAATGGATTGTTCGGTGGAAAATTAGGAGTCGAGGAATTAGTTCTCGCCGGGTTAAAATCGGAAGAGAAGGTTTCTGGATACCATGCGGACAATGTCGCACCGGCTATTATGGGGGGATTCATTCTGATTAGGAATTACGAACCCTTGGAATTGATCCGCTTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTCAGCCCAGAATTCGAAGCTCCGACGAAGAAAATGCGGGCTGCTTTGCCGGCTGAAGTTGGGATGCCGCACCATGTATGGAATTCCAGCCAAGCCGGGGCGTTGGTAGCTGCAGTGCTGCAGGGCGACGCAATGGGATTGGGGAAAGCATTGTCGTCAGACAAAATCGTGGAGCCAAGGCGGTCGCCATTGATTCCAGGGATGGATGGGGTTAAGAAGGCAGCCATTGCTGCCGGGGCATTTGGGTGTACAATAAGCGGGGCGGGGCCAACGGCGGTGGCGGTAACCGACAACGAAGAGAAGGGGAAGGAGATTGGTGAGAGGATGGTTATGGCGTTTATGAAGGAAGGGAATTTGAAAGCTGAGGCATCTGTAAAGAGACTGGATCGAGTTGGTGCAAGGCTTATTGGATCAACTCCATTAGATAGAGTTTTATGA

Coding sequence (CDS)

ATGGCTATGATCTCCTATCAACCGCCATTGAAGTCGTTGGCGATTCCTCCAGTTTCTTTATCTAACCCTAAATCTGTCCTCTTCAGGTGCAGTTTGTCTCTTCCATCCAGAACCGCCGTCACTTCCGTTGAACCTGAACCAGTATTCTCCTCCGTGAAGGCATTCGCACCTGCAACCGTCGCTAATTTAGGCCCTGGCTTCGATTTCCTTGGCTGTGCCGTCGATGGCTTGGGAGATTATGTCTCTCTCAGCGTTGACTCCAATGTTCATCCAGGTGAAGTTGCAATTACCAATATTACAGGAAATAACACTAATAAACTTAGTAAAAATCCTCTGTATAATTGCGCTGGAATTGCTGCCATTGAGGTTATGAAAATGTTAGGGATTCGATCTGTCGGTCTCTCTCTTTCGCTTGAGAAAGGTTTGCCGTTGGGGAGTGGATTGGGATCTAGTGCAGCGAGTGCAGCCGCTGCGGCGATTGCTGTTAATGGATTGTTCGGTGGAAAATTAGGAGTCGAGGAATTAGTTCTCGCCGGGTTAAAATCGGAAGAGAAGGTTTCTGGATACCATGCGGACAATGTCGCACCGGCTATTATGGGGGGATTCATTCTGATTAGGAATTACGAACCCTTGGAATTGATCCGCTTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTCAGCCCAGAATTCGAAGCTCCGACGAAGAAAATGCGGGCTGCTTTGCCGGCTGAAGTTGGGATGCCGCACCATGTATGGAATTCCAGCCAAGCCGGGGCGTTGGTAGCTGCAGTGCTGCAGGGCGACGCAATGGGATTGGGGAAAGCATTGTCGTCAGACAAAATCGTGGAGCCAAGGCGGTCGCCATTGATTCCAGGGATGGATGGGGTTAAGAAGGCAGCCATTGCTGCCGGGGCATTTGGGTGTACAATAAGCGGGGCGGGGCCAACGGCGGTGGCGGTAACCGACAACGAAGAGAAGGGGAAGGAGATTGGTGAGAGGATGGTTATGGCGTTTATGAAGGAAGGGAATTTGAAAGCTGAGGCATCTGTAAAGAGACTGGATCGAGTTGGTGCAAGGCTTATTGGATCAACTCCATTAGATAGAGTTTTATGA

Protein sequence

MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATVANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVGARLIGSTPLDRVL
Homology
BLAST of Lsi11G012940 vs. ExPASy Swiss-Prot
Match: Q8L7R2 (Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 7.1e-137
Identity = 268/371 (72.24%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MAMISYQPPLKSLA-IPPVSLSNP----KSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAF 60
           MA + +Q P K ++   P S  +P    K  +FRC  S+ +   + +VEPEPVF SVK F
Sbjct: 1   MASLCFQSPSKPISYFQPKSNPSPPLFAKVSVFRCRASVQT---LVAVEPEPVFVSVKTF 60

Query: 61  APATVANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNC 120
           APATVANLGPGFDFLGCAVDGLGD+V+L VD +V  GEV+I+ ITG  T KLS NPL NC
Sbjct: 61  APATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITG-TTTKLSTNPLRNC 120

Query: 121 AGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEEL 180
           AGIAAI  MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAA+AVN +FG KLG ++L
Sbjct: 121 AGIAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQL 180

Query: 181 VLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEA 240
           VLAGL+SE KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVSP+FEA
Sbjct: 181 VLAGLESEAKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVSPDFEA 240

Query: 241 PTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPG 300
           PTKKMRAALP E+ M HHVWNSSQA ALVAAVL+GDA+ LGKALSSDKIVEP R+PLIPG
Sbjct: 241 PTKKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRAPLIPG 300

Query: 301 MDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKR 360
           M+ VKKAA+ AGAFGCTISGAGPTAVAV D+EEKG+ IGE+MV AF K G+LK+ ASVK+
Sbjct: 301 MEAVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSVASVKK 360

Query: 361 LDRVGARLIGS 367
           LD VGARL+ S
Sbjct: 361 LDNVGARLVNS 367

BLAST of Lsi11G012940 vs. ExPASy Swiss-Prot
Match: B3QWX6 (Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=517418 GN=thrB PE=3 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 5.2e-47
Identity = 133/320 (41.56%), Postives = 183/320 (57.19%), Query Frame = 0

Query: 52  VKAFAPATVANLGPGFDFLGCAVDGLGDYVSLS-VDSNVHPGEVAITNITGNNTNKLSKN 111
           V  FAPA+V N+  GFD LG A+   GD V++S  D    P +V I+ I G+    L  +
Sbjct: 4   VTGFAPASVGNVACGFDVLGFALTEPGDEVTVSFTDDENSPAQVIISKIIGDG-GALPTD 63

Query: 112 PLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNGL 171
           P  N A I  I+ ++ L    GI   G  S+ L+K LPL SG+GSSA+SAAAA IA N L
Sbjct: 64  PYKNTASIVVIKFLEFLKTHKGIEQSGTFSIELKKNLPLSSGMGSSASSAAAALIAANSL 123

Query: 172 FGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKEL 231
            G      ELV   ++ E    G  HADN APA++G FIL R+Y+PL+LI +  P   EL
Sbjct: 124 LGSPCTKMELVPFVMEGERIACGSIHADNAAPAMLGNFILTRSYDPLDLIPISTP--PEL 183

Query: 232 FFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKI 291
           +  LV P  E PT   R+ L  E+ +   V      GALVA +L+ D   +G+AL  D +
Sbjct: 184 YCSLVHPHIEVPTSHARSILKKEIALSSAVRQWGNVGALVAGLLRCDYELIGRAL-EDVV 243

Query: 292 VEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKE 351
            EP R+PLIPG   VK AA+ AGA G +I+G+GP+  A +D+  K + I + M   F K 
Sbjct: 244 AEPVRAPLIPGFYDVKHAALDAGALGGSIAGSGPSIFAFSDSLAKAETIAKAMQETFRKV 303

Query: 352 GNLKAEASVKRLDRVGARLI 365
            NL+++     + R G+R++
Sbjct: 304 SNLESDIWFCPVSREGSRIL 319

BLAST of Lsi11G012940 vs. ExPASy Swiss-Prot
Match: A4SGN0 (Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=290318 GN=thrB PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.0e-46
Identity = 133/322 (41.30%), Postives = 187/322 (58.07%), Query Frame = 0

Query: 51  SVKAFAPATVANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPG-EVAITNITGNNTNKLSK 110
           SV+ FA ATV N+  GFD LG A+   GD V L+     H G  V I++I+G+   KL  
Sbjct: 3   SVRGFASATVGNVACGFDILGFAITEPGDEVILTRSERKHTGCPVTISSISGDG-GKLPL 62

Query: 111 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNG 170
           +P  N +    ++ ++ +    G    G +SL L+K LPL SG+GSSAASAAAA  A N 
Sbjct: 63  DPRKNTSSFVVLKFLEHIRTHKGQDCTGHISLELKKHLPLSSGMGSSAASAAAALAAANE 122

Query: 171 LFGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 230
           L G      ELV   ++ E    G  HADN APAI+G F+LIR+Y PL+LI +  P  ++
Sbjct: 123 LLGRPCTKMELVHFAIEGERVACGSAHADNAAPAILGNFVLIRSYTPLDLIVI--PPPEK 182

Query: 231 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDK 290
           LF  LV P  E  T   R+ LP ++ +          GALVA +L  D   +G++L  D 
Sbjct: 183 LFCSLVHPHTELRTSFARSVLPTDITLKDATRQWGNVGALVAGLLTSDYALVGRSL-VDV 242

Query: 291 IVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFM- 350
           + EP+R+PLIPG   VK+AA+ AGA GC+I+G+GP+  A +D+ E     GE M  AF+ 
Sbjct: 243 VAEPKRAPLIPGFAEVKEAALNAGALGCSIAGSGPSIFAFSDSAETAHAAGEAMQKAFLA 302

Query: 351 KEGNLKAEASVKRLDRVGARLI 365
            + +LK++  V  + R GAR++
Sbjct: 303 SKKSLKSDMWVSPICREGARIL 320

BLAST of Lsi11G012940 vs. ExPASy Swiss-Prot
Match: Q8KAW7 (Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=thrB PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.4e-46
Identity = 132/325 (40.62%), Postives = 186/325 (57.23%), Query Frame = 0

Query: 51  SVKAFAPATVANLGPGFDFLGCAVDGLGDYVSLSV-DSNVHPGEVAITNITGNNTNKLSK 110
           +V  FA ATV N+  GFD LG A+   GD V L++ D       V+IT+I G+    L  
Sbjct: 3   TVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDG-GALPL 62

Query: 111 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNG 170
           +P  N +    ++ ++ +    GI   G + L L+K LPL SG+GSSAASAAAA IA N 
Sbjct: 63  DPKKNTSSFVVLKFLEYIRTTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAANE 122

Query: 171 LFGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 230
           LFG      ELV   ++ E    G  HADN APA++G FILIR+Y PL+LI +K P  K 
Sbjct: 123 LFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNFILIRSYNPLDLITIKPP--KN 182

Query: 231 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDK 290
           LF  LV P  E  T   R+ LP  + +          GAL+A +L  D   +G+AL  D 
Sbjct: 183 LFGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRAL-VDV 242

Query: 291 IVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFM- 350
           + EP+R+PLIPG + VK+AA+ AGA GC+I+G+GP+  A + + +  + +G  M  AF+ 
Sbjct: 243 VAEPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLH 302

Query: 351 KEGNLKAEASVKRLDRVGARLIGST 368
               L+++  V  +   GAR+I +T
Sbjct: 303 SRAALQSDMWVSPICSQGARIISTT 323

BLAST of Lsi11G012940 vs. ExPASy Swiss-Prot
Match: A1BJF6 (Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=thrB PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.9e-44
Identity = 128/322 (39.75%), Postives = 185/322 (57.45%), Query Frame = 0

Query: 51  SVKAFAPATVANLGPGFDFLGCAVDGLGDYVSLSV-DSNVHPGEVAITNITGNNTNKLSK 110
           +V+ FA ATV N+  GFD LG A+   GD V L++ +       V+IT I+G+    L  
Sbjct: 3   TVRGFASATVGNVACGFDVLGFAITEPGDEVILTLHEERKSECPVSITAISGDG-GALPL 62

Query: 111 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAIAVNG 170
           +P  N +    ++ ++ +    GI   G + L L+K LPL SG+GSSAASAAAA  A N 
Sbjct: 63  DPKKNTSSFVVLKFLEYIRTSKGIDFKGHIDLELKKNLPLSSGMGSSAASAAAALAAANE 122

Query: 171 LFGGKLGVEELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 230
           L G      ELV   ++ E    G  HADN APAI+G F+LIR+Y PL+LI +  P  + 
Sbjct: 123 LLGQPCSKMELVHFAVEGERVACGSAHADNAAPAILGNFVLIRSYAPLDLITI--PSPEH 182

Query: 231 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDK 290
           LF  LV P  E  T   R+ LP  + +          GAL+A +L+ D   +G+AL  D 
Sbjct: 183 LFCTLVHPHTELKTSFARSVLPRSIPLKTATQQWGNVGALIAGLLKSDYDLIGRAL-VDV 242

Query: 291 IVEPRRSPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAF-M 350
           + EP+R+PLIPG   VK AAI  GA GC+I+G+GP+  A + ++E  + +G+ M  AF +
Sbjct: 243 VAEPKRAPLIPGFLDVKHAAIDCGALGCSIAGSGPSVFAFSSSKETAERVGQAMREAFLL 302

Query: 351 KEGNLKAEASVKRLDRVGARLI 365
            E NLK++  V  + + GA+++
Sbjct: 303 PETNLKSDMWVSPICKEGAKVL 320

BLAST of Lsi11G012940 vs. ExPASy TrEMBL
Match: A0A0A0K4Y4 (Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.8e-195
Identity = 363/373 (97.32%), Postives = 368/373 (98.66%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAM+SYQPPLKSL IPPVSLSNPK VLFRCSLSLPSRTAVTSVEP+PVFSSVKAFAPATV
Sbjct: 1   MAMLSYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRTAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAI++ITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEEKGKEIGERMVMAF+KEGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. ExPASy TrEMBL
Match: A0A5A7V1E5 (Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00200 PE=3 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 3.9e-194
Identity = 362/373 (97.05%), Postives = 367/373 (98.39%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAMISYQPPLKSL IPPVSLSNPK VLFRCSLSLPSR AVTSVEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAI++ITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEEKGKEIGERMVMAF+KEGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. ExPASy TrEMBL
Match: A0A1S3BR18 (Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.1e-193
Identity = 361/373 (96.78%), Postives = 367/373 (98.39%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAMISYQPPLKSL IPPVSLSNPK VLFRCSLSLPSR AVTSVEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAI++ITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEE+GKEIGERMVMAF+KEGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. ExPASy TrEMBL
Match: A0A6J1C3W0 (Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.5e-185
Identity = 344/365 (94.25%), Postives = 357/365 (97.81%), Query Frame = 0

Query: 4   ISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATVANL 63
           I +QP LK +A+PPVSLSNPKSVLFRC+LSLPSRTAVT+VEPEPVFSSVKAFAPATVANL
Sbjct: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62

Query: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAAIEV 123
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAITNI+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122

Query: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182

Query: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242

Query: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSP+IPGMDGVKKAA
Sbjct: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKKAA 302

Query: 304 IAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVGARL 363
           IAAGAFGCTISGAGPTAVAV D+EE+GKEIGERMVMAF+KEGNLKA A VKRLDRVGARL
Sbjct: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGARL 362

Query: 364 IGSTP 369
           +GSTP
Sbjct: 363 VGSTP 367

BLAST of Lsi11G012940 vs. ExPASy TrEMBL
Match: A0A6J1GJP3 (Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.6e-182
Identity = 342/372 (91.94%), Postives = 354/372 (95.16%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAMIS+QPPLKS A+P V L NPKSV   CSLSLPSRT VT+VEP PVF+SVKAFAPATV
Sbjct: 1   MAMISFQPPLKSFAVPQVPLYNPKSVAVCCSLSLPSRTVVTAVEPAPVFASVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD+VSLSVDSNV PGEVAITNITGN+ NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDFVSLSVDSNVRPGEVAITNITGNDPNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVE+LVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEDLVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEP R PLIPGM GVK
Sbjct: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDVIGLGKALSSDKIVEPTRVPLIPGMGGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAVTDN+E+GKEIGE MVMAFM+EGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVTDNKERGKEIGESMVMAFMREGNLKAVASVKRLDRVG 360

Query: 361 ARLIGSTPLDRV 373
           ARLIGSTP+DRV
Sbjct: 361 ARLIGSTPIDRV 372

BLAST of Lsi11G012940 vs. NCBI nr
Match: XP_038888998.1 (homoserine kinase [Benincasa hispida])

HSP 1 Score: 701.4 bits (1809), Expect = 4.1e-198
Identity = 370/373 (99.20%), Postives = 372/373 (99.73%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAMISYQPPLKSLAIPPVSLSNPKSV+FRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLAIPPVSLSNPKSVIFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALP+EVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPSEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. NCBI nr
Match: XP_004153462.1 (homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 [Cucumis sativus])

HSP 1 Score: 689.5 bits (1778), Expect = 1.6e-194
Identity = 363/373 (97.32%), Postives = 368/373 (98.66%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAM+SYQPPLKSL IPPVSLSNPK VLFRCSLSLPSRTAVTSVEP+PVFSSVKAFAPATV
Sbjct: 1   MAMLSYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRTAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAI++ITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEEKGKEIGERMVMAF+KEGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. NCBI nr
Match: KAA0059609.1 (homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucumis melo var. makuwa])

HSP 1 Score: 687.2 bits (1772), Expect = 8.0e-194
Identity = 362/373 (97.05%), Postives = 367/373 (98.39%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAMISYQPPLKSL IPPVSLSNPK VLFRCSLSLPSR AVTSVEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAI++ITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEEKGKEIGERMVMAF+KEGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. NCBI nr
Match: XP_008451216.1 (PREDICTED: homoserine kinase [Cucumis melo])

HSP 1 Score: 685.6 bits (1768), Expect = 2.3e-193
Identity = 361/373 (96.78%), Postives = 367/373 (98.39%), Query Frame = 0

Query: 1   MAMISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60
           MAMISYQPPLKSL IPPVSLSNPK VLFRCSLSLPSR AVTSVEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAI++ITGNNTNKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300
           RAALPAEVGMPHHVWNSSQAGALVAAVLQGD MGLGKALSSDKIVEPRRSPLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DNEE+GKEIGERMVMAF+KEGNLKA ASVKRLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTPLDRVL 374
           ARLIGSTPLDRVL
Sbjct: 361 ARLIGSTPLDRVL 373

BLAST of Lsi11G012940 vs. NCBI nr
Match: XP_022135832.1 (homoserine kinase [Momordica charantia])

HSP 1 Score: 658.7 bits (1698), Expect = 3.0e-185
Identity = 344/365 (94.25%), Postives = 357/365 (97.81%), Query Frame = 0

Query: 4   ISYQPPLKSLAIPPVSLSNPKSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATVANL 63
           I +QP LK +A+PPVSLSNPKSVLFRC+LSLPSRTAVT+VEPEPVFSSVKAFAPATVANL
Sbjct: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62

Query: 64  GPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAAIEV 123
           GPGFDFLGCAVDGLGD+VSLSVDSNVHPGEVAITNI+GNNTNKLSKNPLYNCAGIAAIEV
Sbjct: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122

Query: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 183
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE
Sbjct: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182

Query: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242

Query: 244 LPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVKKAA 303
           LPAEVGMPHHVWN SQAGALVAAVLQGD +GLGKALSSDKIVEPRRSP+IPGMDGVKKAA
Sbjct: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKKAA 302

Query: 304 IAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVGARL 363
           IAAGAFGCTISGAGPTAVAV D+EE+GKEIGERMVMAF+KEGNLKA A VKRLDRVGARL
Sbjct: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGARL 362

Query: 364 IGSTP 369
           +GSTP
Sbjct: 363 VGSTP 367

BLAST of Lsi11G012940 vs. TAIR 10
Match: AT2G17265.1 (homoserine kinase )

HSP 1 Score: 488.4 bits (1256), Expect = 5.1e-138
Identity = 268/371 (72.24%), Postives = 308/371 (83.02%), Query Frame = 0

Query: 1   MAMISYQPPLKSLA-IPPVSLSNP----KSVLFRCSLSLPSRTAVTSVEPEPVFSSVKAF 60
           MA + +Q P K ++   P S  +P    K  +FRC  S+ +   + +VEPEPVF SVK F
Sbjct: 1   MASLCFQSPSKPISYFQPKSNPSPPLFAKVSVFRCRASVQT---LVAVEPEPVFVSVKTF 60

Query: 61  APATVANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNC 120
           APATVANLGPGFDFLGCAVDGLGD+V+L VD +V  GEV+I+ ITG  T KLS NPL NC
Sbjct: 61  APATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITG-TTTKLSTNPLRNC 120

Query: 121 AGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEEL 180
           AGIAAI  MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAA+AVN +FG KLG ++L
Sbjct: 121 AGIAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKLGSDQL 180

Query: 181 VLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEA 240
           VLAGL+SE KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVSP+FEA
Sbjct: 181 VLAGLESEAKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVSPDFEA 240

Query: 241 PTKKMRAALPAEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPG 300
           PTKKMRAALP E+ M HHVWNSSQA ALVAAVL+GDA+ LGKALSSDKIVEP R+PLIPG
Sbjct: 241 PTKKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRAPLIPG 300

Query: 301 MDGVKKAAIAAGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKR 360
           M+ VKKAA+ AGAFGCTISGAGPTAVAV D+EEKG+ IGE+MV AF K G+LK+ ASVK+
Sbjct: 301 MEAVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSVASVKK 360

Query: 361 LDRVGARLIGS 367
           LD VGARL+ S
Sbjct: 361 LDNVGARLVNS 367

BLAST of Lsi11G012940 vs. TAIR 10
Match: AT4G35295.1 (homoserine kinase, putative / HSK, putative )

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-12
Identity = 41/61 (67.21%), Postives = 46/61 (75.41%), Query Frame = 0

Query: 306 AGAFGCTISGAGPTAVAVTDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVGARLIG 365
           AGAFG TI     TAVAV D  EKG EIGE+MV  F+K GNLK+ ASVK+LD+VGARLI 
Sbjct: 55  AGAFGRTI-----TAVAVIDTAEKGYEIGEKMVQVFLKIGNLKSTASVKKLDKVGARLIE 110

Query: 366 S 367
           S
Sbjct: 115 S 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7R27.1e-13772.24Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1[more]
B3QWX65.2e-4741.56Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=51... [more]
A4SGN02.0e-4641.30Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=29031... [more]
Q8KAW74.4e-4640.62Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC... [more]
A1BJF61.9e-4439.75Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=t... [more]
Match NameE-valueIdentityDescription
A0A0A0K4Y47.8e-19597.32Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1[more]
A0A5A7V1E53.9e-19497.05Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00... [more]
A0A1S3BR181.1e-19396.78Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1[more]
A0A6J1C3W01.5e-18594.25Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1[more]
A0A6J1GJP32.6e-18291.94Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888998.14.1e-19899.20homoserine kinase [Benincasa hispida][more]
XP_004153462.11.6e-19497.32homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 ... [more]
KAA0059609.18.0e-19497.05homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucu... [more]
XP_008451216.12.3e-19396.78PREDICTED: homoserine kinase [Cucumis melo][more]
XP_022135832.13.0e-18594.25homoserine kinase [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G17265.15.1e-13872.24homoserine kinase [more]
AT4G35295.13.3e-1267.21homoserine kinase, putative / HSK, putative [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00958HOMSERKINASEcoord: 149..164
score: 45.83
coord: 188..201
score: 52.78
coord: 228..245
score: 38.89
coord: 59..74
score: 61.11
coord: 308..323
score: 47.92
NoneNo IPR availablePANTHERPTHR20861HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASEcoord: 11..366
NoneNo IPR availablePANTHERPTHR20861:SF8BNAA09G09000D PROTEINcoord: 11..366
IPR036554GHMP kinase, C-terminal domain superfamilyGENE3D3.30.70.890coord: 222..353
e-value: 3.5E-37
score: 129.2
IPR036554GHMP kinase, C-terminal domain superfamilySUPERFAMILY55060GHMP Kinase, C-terminal domaincoord: 223..337
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3D3.30.230.10coord: 46..221
e-value: 7.3E-46
score: 157.8
IPR000870Homoserine kinaseTIGRFAMTIGR00191TIGR00191coord: 54..363
e-value: 3.4E-65
score: 217.8
IPR000870Homoserine kinaseHAMAPMF_00384Homoser_kinasecoord: 52..364
score: 27.401833
IPR013750GHMP kinase, C-terminal domainPFAMPF08544GHMP_kinases_Ccoord: 264..337
e-value: 1.5E-9
score: 38.0
IPR006204GHMP kinase N-terminal domainPFAMPF00288GHMP_kinases_Ncoord: 136..201
e-value: 3.5E-10
score: 40.0
IPR006203GHMP kinase, ATP-binding, conserved sitePROSITEPS00627GHMP_KINASES_ATPcoord: 142..153
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILY54211Ribosomal protein S5 domain 2-likecoord: 51..219

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G012940.1Lsi11G012940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0016310 phosphorylation
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006566 threonine metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004413 homoserine kinase activity
molecular_function GO:0015276 ligand-gated ion channel activity