Lsi11G012240 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi11G012240 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 6.5e-249
Identity = 495/999 (49.55%), Postives = 631/999 (63.16%), Query Frame = 0

Query: 56   DPSDRTEVILKID--DGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDA--IGIGESASR 115
            D +DR EVI+KID  +G ++ VS      G   GK+WR+  YDFW +    +  G +A+ 
Sbjct: 4    DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 116  VSGARTSDSGVD--RNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEEL 175
            V   R++ +  +  ++EGFEF +     EDPPTKLIG+FLHKQ+  GE  LDMDL M+EL
Sbjct: 64   VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 176  R----PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQPPWQQ 235
            +     P++ESP   T +D  V  +   +  ++ND                         
Sbjct: 124  QSRGLTPVSESPRVSTKRD-PVGRRDSRSNTNNNDD------------------------ 183

Query: 236  SHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSH-LLRAKTKSRLIDPPA-- 295
                                E ++C+ N         QR S  LL+ +T+SRL DPP   
Sbjct: 184  -------------------GEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPTPQ 243

Query: 296  ----EPDRLSGLIPKSGQLRSGFLGKI-----DDDDDDPFLDEDLPDDFKRGNFNALTVL 355
                  D  SG IPKSGQ++SGF GK      ++++DDPF  EDLP+++++   +   VL
Sbjct: 244  LPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVL 303

Query: 356  QWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIE 415
            +W+SLILI A  VCTL+IP LR+K LWEL +WKWE MVL+LICGRLVS W ++IVVFFIE
Sbjct: 304  EWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIE 363

Query: 416  RNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCL 475
            RNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K   T  L  V ++ VCL
Sbjct: 364  RNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCL 423

Query: 476  LISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEER 535
            L+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPLIE++KNEEEEER
Sbjct: 424  LVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEER 483

Query: 536  IADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNG--- 595
            I+ EV+  QN G                 G  I SG       T  S F   +  NG   
Sbjct: 484  ISVEVKKFQNPG-----------------GVEIQSGAQKSPMKTGKSPFLSHVLSNGGGG 543

Query: 596  ---NDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTEIK-- 655
               N GITID LHKL+PKNVSAW MKRL+NI+R GS++TLDEQ+  P +D++   +I+  
Sbjct: 544  GGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSE 603

Query: 656  ---KLGG--LSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSALKNW 715
               KL    +   +++   K+IY +D+MRF+ +DE  KT+SLFEGA E  RISKS+LKNW
Sbjct: 604  FEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNW 663

Query: 716  VKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCV 775
            V                                                           
Sbjct: 664  V----------------------------------------------------------- 723

Query: 776  LCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATSKFFV 835
                   VNAFRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI ++KF V
Sbjct: 724  -------VNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLV 783

Query: 836  FVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLL 895
             +SSQ+VVVAFIFGN CK +FE++I+LFV+HPFDVGDRCEIDG+Q               
Sbjct: 784  VMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQ--------------- 835

Query: 896  NLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSV 955
                      MVVEEMNILTTVFLR+DN K++ PNS+L TK I N+YRSPDMG+ +EFS+
Sbjct: 844  ----------MVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSI 835

Query: 956  HIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSG 1015
            HI TPAEKI  +KQRI SYIEG K+HWYP+PMIVFKD++ LN +++AVW +HRMNHQD G
Sbjct: 904  HITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMG 835

Query: 1016 ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSS 1020
            E+WARRS LVEE+ K+C+ELDI+YRL P+DIN+R+LP+S
Sbjct: 964  EKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLPTS 835

BLAST of Lsi11G012240 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 826.2 bits (2133), Expect = 4.0e-238
Identity = 493/1061 (46.47%), Postives = 660/1061 (62.21%), Query Frame = 0

Query: 4    SFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDRTEV 63
            SFK + S+K   +I + G  SE + E LPIL +H P DH   +     V+D  P      
Sbjct: 6    SFKSHSSYK---QIRSPGDQSEPSPEHLPILHDHHP-DHSGMV-----VDDQKPDSTRS- 65

Query: 64   ILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSDSGV- 123
               +DDG ++ V               R++ Y FW ++  G     + V   RTSD    
Sbjct: 66   --SLDDGRNAPVE--------------RDASYKFWQDNTTGTSTDHTAV---RTSDKDPI 125

Query: 124  ------DRNEG-FEFAQPGYGMEDPPTKLI-GEFLHKQ---KMRGETTLDMDLEMEELRP 183
                  DR  G F+F      +++ PTK++ GE +++Q   +   E TLD+D E +++  
Sbjct: 126  AISRKGDRLSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSH 185

Query: 184  PLAESPLS------QTSKDLKVSFQ--------------QDSTEISSNDQSMRRRYRDSH 243
                +P S        S++++VSF                 S+  SS+  +  R  +D  
Sbjct: 186  QTMPTPTSTARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQP 245

Query: 244  DLQEEYKGQPPWQQSHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLL-R 303
             LQEE                             E +RC SN      +SFQRKS L+ R
Sbjct: 246  QLQEE-----------------------------EVVRCTSN------MSFQRKSELISR 305

Query: 304  AKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNA 363
             KT+SRL DPP E +   SG   +SGQL+SG L  I D++DDP  +ED+PD++KRG  +A
Sbjct: 306  VKTRSRLQDPPREEETPYSGW--RSGQLKSGLLADI-DEEDDPLAEEDVPDEYKRGKLDA 365

Query: 364  LTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVV 423
            +T+LQW+SL+ I AAL C+LSI   ++  +W L +WKWEV +L+LICGRLVSGWGIRIVV
Sbjct: 366  ITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVV 425

Query: 424  FFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRV 483
            FFIERNFLLRKRVLYFVYGVR+ VQNCLWLGLVL+AWH LF+K+V+++T++  L YV ++
Sbjct: 426  FFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKI 485

Query: 484  LVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEE 543
            LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP+IE+ + EE
Sbjct: 486  LVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEE 545

Query: 544  EEERIADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKN 603
            EEER  DE+  +QNAG N+PPDL AA F   KSGR++            + K S  +PK+
Sbjct: 546  EEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVM------------NPKLSPIIPKS 605

Query: 604  GND-GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTEI---K 663
              D GI+++HLH+++ KN+SAWNMKRL+ IVR  S++TLDEQ+     ++EST +I   K
Sbjct: 606  TTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEK 665

Query: 664  KLGGLSREISEGTR----KYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSALKNWV 723
            +    +R+I +       KYIYL+DLMRF+REDE  KTM LFEGA EN+RISKSALKNW+
Sbjct: 666  EAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWL 725

Query: 724  KAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVL 783
                                                                        
Sbjct: 726  ------------------------------------------------------------ 785

Query: 784  CIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATSKFFVF 843
                  VNAFRERRALALTLNDTKTAV+KLHHM+N++  I+I+++WL++L IA+SK  +F
Sbjct: 786  ------VNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLF 845

Query: 844  VSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLN 903
            VSSQ+V++AFIFGNT KT+FE++IFLF++HP+DVGDRCEID +Q                
Sbjct: 846  VSSQVVLLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQ---------------- 896

Query: 904  LGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVH 963
                     +VVEEMNILTTVFLRYDNLKI+ PNS+L  K I+N+YRSPDMG+++EF VH
Sbjct: 906  ---------LVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNYYRSPDMGDAIEFCVH 896

Query: 964  IATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGE 1023
            I TP EKI+ +KQRI +YI+   E+WYP   I+ KD++ L++++LA+W  HR+NHQD  E
Sbjct: 966  ITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPCHRINHQDMAE 896

BLAST of Lsi11G012240 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 810.4 bits (2092), Expect = 2.3e-233
Identity = 466/1004 (46.41%), Postives = 628/1004 (62.55%), Query Frame = 0

Query: 56   DPSDRTEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGA 115
            D +DR + I+ I+   S AV  +   + + GG +W+ES YDFW+ +              
Sbjct: 5    DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK------------G 64

Query: 116  RTSDSGVDRNE---GFEFAQPG------YGMEDPPTKLIGEFLHKQKMRG-ETTLDMDLE 175
            +    G D +E    F F Q G        + DPP+KLIG+FLHKQ+  G E +LD++L 
Sbjct: 65   KNDKKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELN 124

Query: 176  MEELRPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQPPWQQ 235
            M EL+      P + ++       ++  T IS +          S  ++ + K     ++
Sbjct: 125  MAELQSNTPPRPATASNTP-----RRGLTTISES----------SSPVKTKVKADAVRRR 184

Query: 236  SHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPA--- 295
             +   LG  +    +N   AE ++C S           +K  L R KTKSRL DPP    
Sbjct: 185  QNRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTH 244

Query: 296  ----EPDRLSGLIPKSGQLRSGFLGKI------------DDDDDDPFLDEDLPDDFKRGN 355
                + +  SG   +SG  +SGFLGK             +++++DPFLDEDLP++FKR  
Sbjct: 245  PAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDK 304

Query: 356  FNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIR 415
             +    L+W+SL+LI  +LVC+L+I  L+ K+ W+LD+WKWEV VL+LICGRLVS W +R
Sbjct: 305  LSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVR 364

Query: 416  IVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYV 475
            I+VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T+++ L YV
Sbjct: 365  IIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYV 424

Query: 476  RRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRK 535
             RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+E+++
Sbjct: 425  TRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQR 484

Query: 536  NEEEEERIADEVQNLQN-AGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTKSSKFSQA 595
             EEEE+++A++V++L+  AG  +PP LKA   + +K G+  G  R               
Sbjct: 485  MEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGS------------ 544

Query: 596  LPKNGND--GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTE 655
              K G D  GI ID L +++ KNVSAWNMKRL+NI+  G+ISTLD+ +     ++E  T 
Sbjct: 545  --KRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATH 604

Query: 656  IKK-------LGGLSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSA 715
            I+           +   ++E   +YIYL+D +RF+ E+E  + M+LFEGA E+ +ISKS 
Sbjct: 605  IRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSC 664

Query: 716  LKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISS 775
            LKNWV                                                       
Sbjct: 665  LKNWV------------------------------------------------------- 724

Query: 776  KSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATS 835
                       V AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIAT+
Sbjct: 725  -----------VKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 784

Query: 836  KFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTT 895
            +F + +SSQ+++VAF+FGN+CKTIFEA+IFLFVMHPFDVGDRCEIDG+Q           
Sbjct: 785  RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQ----------- 844

Query: 896  IVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESV 955
                          +VVEEMNILTTVFLRYDN KII PNSVL TK I N+YRSPDMG++V
Sbjct: 845  --------------LVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAV 863

Query: 956  EFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNH 1015
            EF VHIATP EKITA+KQRI+SY++  K++WYP+PMIVF  +D LN +K+AVWL+HRMNH
Sbjct: 905  EFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNH 863

Query: 1016 QDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSA 1021
            QD GER+ RR +L+EEV K C+ELDI+YRL P++IN+RSLP +A
Sbjct: 965  QDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTA 863

BLAST of Lsi11G012240 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 8.3e-220
Identity = 447/1007 (44.39%), Postives = 623/1007 (61.87%), Query Frame = 0

Query: 51   AVNDSDPSDRTEVILKID--DGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGES 110
            AV+ +D   R + +++ID  D G S              K WRES  +FW+ND       
Sbjct: 2    AVDSTD--QRRDFVVRIDGEDNGDSE-------------KFWRESSINFWHND------K 61

Query: 111  ASRVSGARTSDSGVD--RNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRG-ETTLDMDLE 170
            +S+  G    D   D  R    +  +P     DPP+KLI +FL+KQK  G E +LDM+  
Sbjct: 62   SSKPPGGEEDDGSFDFMRRSSEKSEEP-----DPPSKLINQFLNKQKASGDEISLDMEAN 121

Query: 171  MEELR----PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQP 230
            M EL+    PPL+ + +S ++    V+    ++  +    ++RRR       Q      P
Sbjct: 122  MPELQKNTVPPLSSTAVSGSAS--PVTAPVTASYRNGTGDAIRRR-------QNRVTLSP 181

Query: 231  PWQQSHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPP 290
              +           + G      +E ++C SN       S  R   L++ KT+SRL+DPP
Sbjct: 182  SVKDGDSSEDEENRVDG------SEVVKCTSN------RSTMRTKTLMKMKTRSRLMDPP 241

Query: 291  AE--PDRLSGLIPKSGQLRSGFLG-------------KIDDDDDDPFLDEDLPDDFKRGN 350
                PD +SG  P+SG L  GF G             K  ++++DPF +EDLP+  ++  
Sbjct: 242  TPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEK 301

Query: 351  FNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIR 410
                 +++W+ LILI A+L+C+L IPYLR K+LW+L +WKWEVMVL+LICGRLVS W ++
Sbjct: 302  ICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVK 361

Query: 411  IVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYV 470
            + V+F+E NFL RK+VLYFVYG+RKPVQNCLWLGLVL+AWH LF+K+VE++ ++++L YV
Sbjct: 362  LFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYV 421

Query: 471  RRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRK 530
             +VL+CLL++ +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP IE+  
Sbjct: 422  TKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI-- 481

Query: 531  NEEEEERIADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHK--RFCTKSSKFSQ 590
               EEE++A++V+  +  G  + P        S      +GSGR  K      KS   S+
Sbjct: 482  -HIEEEKVANDVKTFEIVGRKLSP--LGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSR 541

Query: 591  ALPK--NGNDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCV-DNEST 650
            +  K   G +GI IDHL +++ KNVSAW MK+L+N+++ G++STLDEQI      +++  
Sbjct: 542  SGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKA 601

Query: 651  TEIK-----KLGG--LSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISK 710
            T+I+     KL    + + ++E   +YIY++D MRF+ EDE  + M LFEGA E  +ISK
Sbjct: 602  TQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISK 661

Query: 711  SALKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKI 770
            S LKNWV                                                     
Sbjct: 662  SCLKNWV----------------------------------------------------- 721

Query: 771  SSKSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIA 830
                         VNAFRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA
Sbjct: 722  -------------VNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIA 781

Query: 831  TSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFY 890
            T+KF + +SSQ+++V F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+Q         
Sbjct: 782  TTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQ--------- 841

Query: 891  TTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGE 950
                            M+VEEMNILTTVFLR+DN KI+ PNS+L TK I N+YRSPDM +
Sbjct: 842  ----------------MIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQD 865

Query: 951  SVEFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRM 1010
            ++EF VHIATP EK TA++QRI+SY++  K+HW+PSPMIVF+D+ GLN +K+A+W +H+M
Sbjct: 902  AIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKM 865

Query: 1011 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAP 1022
            NHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++SLP++ P
Sbjct: 962  NHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATP 865

BLAST of Lsi11G012240 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 1.8e-206
Identity = 388/772 (50.26%), Postives = 523/772 (67.75%), Query Frame = 0

Query: 264  RKSHLL-RAKTKSRLIDPPAEPD-RLSGLIPKSGQLRSGFLGKIDDD---DDDPFLDEDL 323
            RK+ L+ RAK +SRLIDPP E + + S  I  S QLRSG LG+  DD   +DD   +ED+
Sbjct: 161  RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 324  PDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGR 383
            P ++++   +A+T+LQW+SLI +  ALV +L +   R  +LW L +WKWEV++L+LICGR
Sbjct: 221  PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 384  LVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQT 443
            LVSG GIRI+VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281  LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 444  KTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 503
            ++ +L  + ++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341  QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 504  PPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTK 563
            PP++EL + EEEE+R  DE+  +Q  G ++ P+L +A F   KSG             T 
Sbjct: 401  PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSG------------STM 460

Query: 564  SSKFSQALPKNGND-GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVD 623
            + KFS  +PK G+D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461  NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 624  NESTTEIKK-------LGGLSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENR 683
            +EST +I+           + + +++   K+IYL+DLMRF+R DE  KTM LFEGA+  +
Sbjct: 521  DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 684  RISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPI 743
            +I+KSALKNW+                                                 
Sbjct: 581  KITKSALKNWL------------------------------------------------- 640

Query: 744  SDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIV 803
                             VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+
Sbjct: 641  -----------------VNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLIL 700

Query: 804  LGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLIC 863
            L IATSK+ +F++SQ+V++AF+FGN+ KT+FE++IFLF++HP+DVGDR  ID        
Sbjct: 701  LEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLID-------- 760

Query: 864  FDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSP 923
                               EMVVEEMNILTTVFLR DNLKI+ PN +L  K IHN+ RSP
Sbjct: 761  -----------------TVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSP 820

Query: 924  DMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWL 983
            DMG+ V   VHI TP EKI A+KQRI SYI+   E+WYP   ++ KD++ LN++++A+WL
Sbjct: 821  DMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWL 829

Query: 984  SHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPS 1023
             H++NHQ+ GER+ RR++L+EEV+K+  ELDIQYR  P+DIN++++P+   S
Sbjct: 881  CHKINHQNMGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSS 829

BLAST of Lsi11G012240 vs. ExPASy TrEMBL
Match: A0A1S3BID3 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=3 SV=1)

HSP 1 Score: 1585.9 bits (4105), Expect = 0.0e+00
Identity = 840/1041 (80.69%), Postives = 876/1041 (84.15%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGG  SEINHEELPILLNH+  DH       H VNDSDPSDR
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDPSDR 63

Query: 61   TEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSDS 120
            TEVILKIDDGGSSAVSRS D   +NGGKVWRESRYDFWNND  GIGESASRV GAR SDS
Sbjct: 64   TEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDS 123

Query: 121  GVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLAE 180
            G D NEGF+F Q GYGMEDPPTKLIG+FLHKQK+RGETTLDMDLEMEEL+     PPLAE
Sbjct: 124  GDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAE 183

Query: 181  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKG-QPPWQQSHHERLGSP 240
            SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDL+EE+KG QPPWQQSHHERLGSP
Sbjct: 184  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSP 243

Query: 241  TISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
            TISGVQNE+LAEAMRCASNLSFHSELSFQRKS+LLRAKTKSRL DPPAEPDRLSGLIPKS
Sbjct: 244  TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKS 303

Query: 301  GQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYL 360
            GQLRSGFLGKI+DDDDDPFL++DLPDDFKRGNF ALTVLQW SLILITAA +CTLS+PYL
Sbjct: 304  GQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYL 363

Query: 361  REKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQ 420
            REKSLWELDIWKWEVM+LILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPVQ
Sbjct: 364  REKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQ 423

Query: 421  NCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSF 480
            NCLWLGLVL+AWHLLFNKRVEKQT TSILNYV RVLVCLLISTLIWLVKTLMVKVLASSF
Sbjct: 424  NCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSF 483

Query: 481  HVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKA 540
            HVSTYFDRIQESLFNQYVIETLSGPPL+E+RKNEEEEERIADEVQ LQNAG+ IPPDLK 
Sbjct: 484  HVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLK- 543

Query: 541  ATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMKR 600
            ATFAS+KSGR I S RTHK FC KSSKFS+AL KNGNDGITIDHLHKLSPKNVSAWNMKR
Sbjct: 544  ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKR 603

Query: 601  LLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDDL 660
            LLNIVRYGSISTLDEQI GPC+D+ESTTEIK           + + ++    KYIYLDDL
Sbjct: 604  LLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDL 663

Query: 661  MRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKL 720
            MRFMREDEV KT SLFEGA ENRRISKS LKNWV                          
Sbjct: 664  MRFMREDEVLKTTSLFEGAAENRRISKSVLKNWV-------------------------- 723

Query: 721  MLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTA 780
                                                    VN FRERRALALTLNDTKTA
Sbjct: 724  ----------------------------------------VNVFRERRALALTLNDTKTA 783

Query: 781  VDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFL 840
            VDKLHHMVNVIFGILILILWLIVLGIA+SKFF+F+SSQIVVVAFIFGNTCKTIFEA+IFL
Sbjct: 784  VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 843

Query: 841  FVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYD 900
            FVMHPFDVGDRCEIDG                         T+MVVEEMNILTTVFLRYD
Sbjct: 844  FVMHPFDVGDRCEIDG-------------------------TQMVVEEMNILTTVFLRYD 903

Query: 901  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHW 960
            NLKIIIPNSVLATKLIHNFYRSPDMGESVEF VHIATPAEKITAMKQRIISYIEG+KEHW
Sbjct: 904  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHW 945

Query: 961  YPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLL 1020
             P+PMIVFKDIDGLN LKLAVWLSHRMNHQDS ERWARRSVLVEEVVKVCQELDIQYRLL
Sbjct: 964  CPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLL 945

Query: 1021 PIDINIRSLPSSAPSIGFPSN 1029
            PIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 PIDINIRSLPSSAPSIGFPSN 945

BLAST of Lsi11G012240 vs. ExPASy TrEMBL
Match: A0A5D3DI81 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004470 PE=3 SV=1)

HSP 1 Score: 1583.5 bits (4099), Expect = 0.0e+00
Identity = 839/1041 (80.60%), Postives = 875/1041 (84.05%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGG  SEINHEELPILLNH+  DH       H VNDSDPSDR
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDPSDR 63

Query: 61   TEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSDS 120
            TEVILKIDD GSSAVSRS D   +NGGKVWRESRYDFWNND  GIGESASRV GAR SDS
Sbjct: 64   TEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDS 123

Query: 121  GVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLAE 180
            G D NEGF+F Q GYGMEDPPTKLIG+FLHKQK+RGETTLDMDLEMEEL+     PPLAE
Sbjct: 124  GDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAE 183

Query: 181  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKG-QPPWQQSHHERLGSP 240
            SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDL+EE+KG QPPWQQSHHERLGSP
Sbjct: 184  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSP 243

Query: 241  TISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
            TISGVQNE+LAEAMRCASNLSFHSELSFQRKS+LLRAKTKSRL DPPAEPDRLSGLIPKS
Sbjct: 244  TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKS 303

Query: 301  GQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYL 360
            GQLRSGFLGKI+DDDDDPFL++DLPDDFKRGNF ALTVLQW SLILITAA +CTLS+PYL
Sbjct: 304  GQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYL 363

Query: 361  REKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQ 420
            REKSLWELDIWKWEVM+LILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPVQ
Sbjct: 364  REKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQ 423

Query: 421  NCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSF 480
            NCLWLGLVL+AWHLLFNKRVEKQT TSILNYV RVLVCLLISTLIWLVKTLMVKVLASSF
Sbjct: 424  NCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSF 483

Query: 481  HVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKA 540
            HVSTYFDRIQESLFNQYVIETLSGPPL+E+RKNEEEEERIADEVQ LQNAG+ IPPDLK 
Sbjct: 484  HVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLK- 543

Query: 541  ATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMKR 600
            ATFAS+KSGR I S RTHK FC KSSKFS+AL KNGNDGITIDHLHKLSPKNVSAWNMKR
Sbjct: 544  ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKR 603

Query: 601  LLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDDL 660
            LLNIVRYGSISTLDEQI GPC+D+ESTTEIK           + + ++    KYIYLDDL
Sbjct: 604  LLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDL 663

Query: 661  MRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKL 720
            MRFMREDEV KT SLFEGA ENRRISKS LKNWV                          
Sbjct: 664  MRFMREDEVLKTTSLFEGAAENRRISKSVLKNWV-------------------------- 723

Query: 721  MLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTA 780
                                                    VN FRERRALALTLNDTKTA
Sbjct: 724  ----------------------------------------VNVFRERRALALTLNDTKTA 783

Query: 781  VDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFL 840
            VDKLHHMVNVIFGILILILWLIVLGIA+SKFF+F+SSQIVVVAFIFGNTCKTIFEA+IFL
Sbjct: 784  VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 843

Query: 841  FVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYD 900
            FVMHPFDVGDRCEIDG                         T+MVVEEMNILTTVFLRYD
Sbjct: 844  FVMHPFDVGDRCEIDG-------------------------TQMVVEEMNILTTVFLRYD 903

Query: 901  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHW 960
            NLKIIIPNSVLATKLIHNFYRSPDMGESVEF VHIATPAEKITAMKQRIISYIEG+KEHW
Sbjct: 904  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHW 945

Query: 961  YPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLL 1020
             P+PMIVFKDIDGLN LKLAVWLSHRMNHQDS ERWARRSVLVEEVVKVCQELDIQYRLL
Sbjct: 964  CPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLL 945

Query: 1021 PIDINIRSLPSSAPSIGFPSN 1029
            PIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 PIDINIRSLPSSAPSIGFPSN 945

BLAST of Lsi11G012240 vs. ExPASy TrEMBL
Match: A0A0A0K619 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 PE=3 SV=1)

HSP 1 Score: 1560.8 bits (4040), Expect = 0.0e+00
Identity = 833/1042 (79.94%), Postives = 876/1042 (84.07%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGGAGSEINHEELPILLNH+  DH R LRDRH VNDSD SDR
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDTSDR 63

Query: 61   TEVILKIDDGG-SSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSD 120
            TEVILKID GG SSAVSRS D AG+NGG VWRESRYDFWNND IGIGESA         +
Sbjct: 64   TEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA-----RMGDN 123

Query: 121  SGVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLA 180
            SGVDRNEGFEF Q GYGMEDPPTKLIG+FL KQK+ GETTLDMDLEMEEL+     PPLA
Sbjct: 124  SGVDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLA 183

Query: 181  ESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYK-GQPPWQQSHHERLGS 240
            ESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DL+EE K GQ P QQ HHERLGS
Sbjct: 184  ESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGS 243

Query: 241  PTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPK 300
            PTISGVQNE+LAEAMRCASNLSFHSELSFQRKS+LLRAKTKSRLIDPPAEPDRLSGLIPK
Sbjct: 244  PTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPK 303

Query: 301  SGQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPY 360
            SGQLRSGFLGKI+DDDDDPFL++DLPD+FKRGNF+ALTVLQWVSLI+ITAAL+CTLS+PY
Sbjct: 304  SGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 363

Query: 361  LREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPV 420
            LREKSLWELDIWKWEVM+ ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPV
Sbjct: 364  LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 423

Query: 421  QNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASS 480
            QNCLWLGLVL+AWHLLFNKRVEKQT TSILNYV RVLVCLLISTLIWLVKTLMVKVLASS
Sbjct: 424  QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 483

Query: 481  FHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLK 540
            FHVSTYFDRIQESLFNQYVIETLSGPPL+E+RKNEEEEERIADEVQ LQNAGI IPPDLK
Sbjct: 484  FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 543

Query: 541  AATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMK 600
            AATFAS+KSGR IGSGRTHK FC KS K S+AL KN NDGITIDHLHKLS KNVSAWNMK
Sbjct: 544  AATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMK 603

Query: 601  RLLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDD 660
            RLLNIVRYGSISTLDEQI GPC+D+ESTTEIK           + + ++    KYIYLDD
Sbjct: 604  RLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDD 663

Query: 661  LMRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNK 720
            L+RFMREDEV KTMSLFEGA EN+RISKSALKNWV                         
Sbjct: 664  LVRFMREDEVLKTMSLFEGAAENQRISKSALKNWV------------------------- 723

Query: 721  LMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKT 780
                                                     VNAFRERRALALTLNDTKT
Sbjct: 724  -----------------------------------------VNAFRERRALALTLNDTKT 783

Query: 781  AVDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIF 840
            AVDKLHHMVNVIFGILILILWLIVLGIA+SKFF+F+SSQIVVVAFIFGNTCKTIFEA+IF
Sbjct: 784  AVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIF 843

Query: 841  LFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRY 900
            LFVMHPFDVGDRCEIDGMQ                         MVVEEMNILTTVFLRY
Sbjct: 844  LFVMHPFDVGDRCEIDGMQ-------------------------MVVEEMNILTTVFLRY 903

Query: 901  DNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEH 960
            DNLK+IIPNSVLATKLIHNFYRSPDMGES+EF VHIATPAEKITAMK RIISYIEG+KEH
Sbjct: 904  DNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEH 949

Query: 961  WYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 1020
            WYP+PMIVFKDIDGLN +KLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL
Sbjct: 964  WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 949

Query: 1021 LPIDINIRSLPSSAPSIGFPSN 1029
            LPIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 LPIDINIRSLPSSAPSIGFPSN 949

BLAST of Lsi11G012240 vs. ExPASy TrEMBL
Match: A0A6J1C288 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111007608 PE=3 SV=1)

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 765/1047 (73.07%), Postives = 835/1047 (79.75%), Query Frame = 0

Query: 3    KSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDRTE 62
            KSFK NVSFKHTRKISAGG G+EI+H+ELPILL+HEP   HR  R R ++ND DPSDRTE
Sbjct: 6    KSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRH-RGRRSMNDCDPSDRTE 65

Query: 63   VILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIG-----IGESASRVSGART 122
            VILKIDDGGS+AVSR   P   NGGKVWRE+ YDFWNND  G         ASRVSGAR 
Sbjct: 66   VILKIDDGGSAAVSR---PVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGARA 125

Query: 123  SDSGVDRNEGFEFAQPGYG--MEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR----- 182
            SD+G DRNEGFEF Q GYG  +EDPP KLIGEFLHKQK+RGETTLDMDLEMEEL+     
Sbjct: 126  SDNG-DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII 185

Query: 183  PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKG-QPPWQQSHHE 242
            PPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +LQ+EYKG Q PWQQSHHE
Sbjct: 186  PPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQSHHE 245

Query: 243  RLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSG 302
            R GSPTISGVQN+  AEA RC SNLSF  ELSFQR SHLLRAKTKSRL+DPP EP  LSG
Sbjct: 246  RHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSG 305

Query: 303  LIPKSGQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTL 362
            LIPKSG LRSGFLGK +++DDDPFL+EDLP+DFKR +F+ LT+LQW+SL+LIT ALVCTL
Sbjct: 306  LIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTL 365

Query: 363  SIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGV 422
             IPYLR  SLWEL+IWKWEVMVLILICGRLVSGWGIRI+VF IERNFLLRKR+LYFVYGV
Sbjct: 366  YIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGV 425

Query: 423  RKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKV 482
            RKPVQNC+WLGLVL+AW LLFNKRVE +T  ++L YVRRVLV LL+STLIWLVKTLMVKV
Sbjct: 426  RKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKV 485

Query: 483  LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIP 542
            LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIE+RKNEE EER+ADEVQ LQNAG++IP
Sbjct: 486  LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIP 545

Query: 543  PDLKAATFASVKSGRLIGSGRTHKRFCTKSS--KFSQALPKNGNDGITIDHLHKLSPKNV 602
            PDLKA T +SVKSGR+IG G   K    KSS  K S+ L KNGNDGITIDHLHKLSPKNV
Sbjct: 546  PDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNV 605

Query: 603  SAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRK 662
            SAWNMKRLLN+VRYGSISTLDEQIPGPC+++EST EI+           + + ++    K
Sbjct: 606  SAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELK 665

Query: 663  YIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAI 722
            YIY +DLMRFMREDEV +TMSLFEGA E+RRISKS+LKNWV                   
Sbjct: 666  YIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWV------------------- 725

Query: 723  VFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALT 782
                                                           VNAFRERR+LALT
Sbjct: 726  -----------------------------------------------VNAFRERRSLALT 785

Query: 783  LNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTI 842
            LNDTKTAVD+LHHMVNVIFGILILILWLI+LGIA+SKFF FVSSQIVVVAFIFGNTCKTI
Sbjct: 786  LNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTI 845

Query: 843  FEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILT 902
            FEA+IFLFVMHPFDVGDRCEIDGMQ                         MVVEEMNILT
Sbjct: 846  FEAIIFLFVMHPFDVGDRCEIDGMQ-------------------------MVVEEMNILT 905

Query: 903  TVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYI 962
            TVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGESVEF +HIATPAE I  MKQRIISYI
Sbjct: 906  TVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYI 954

Query: 963  EGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQEL 1022
            EG+K HW PSPM VFKD++ LN L+LAVWLSHRMNHQDSGERWARRSVLVE+VVKVCQEL
Sbjct: 966  EGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQEL 954

Query: 1023 DIQYRLLPIDINIRSLPSSAPSIGFPS 1028
            DIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 1026 DIQYRLLPIDINVHSLPSSAPSMGFTS 954

BLAST of Lsi11G012240 vs. ExPASy TrEMBL
Match: A0A6J1EJZ4 (mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC111435046 PE=4 SV=1)

HSP 1 Score: 1235.7 bits (3196), Expect = 0.0e+00
Identity = 672/1022 (65.75%), Postives = 776/1022 (75.93%), Query Frame = 0

Query: 3    KSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDRTE 62
            KS K N  FKH+RKISAGGAGSEIN EELPILL+H+P D H R + R   ND +PS  +E
Sbjct: 6    KSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEPSGHSE 65

Query: 63   VILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFW-NNDAIGIGESASRVSGARTSDSG 122
            VILK+DDGGSSAVSRSQ+ AG  GGKVWRESRYDFW NND IG G SAS  SGAR SDSG
Sbjct: 66   VILKVDDGGSSAVSRSQESAG-YGGKVWRESRYDFWSNNDGIGTGGSASWASGARASDSG 125

Query: 123  VDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLAES 182
             DRNEGFEF + G G +DPPTKLIG+FLHKQK+RGETTLD+DLEMEELR      P+ +S
Sbjct: 126  -DRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPMMDS 185

Query: 183  PLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYK-GQPPWQQSHHERLGSPT 242
            PLS+ SKDLKVSFQ DSTEISSN +S+RRRY++  ++QEE K GQP W QSHHE  GS +
Sbjct: 186  PLSRASKDLKVSFQHDSTEISSN-ESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS-S 245

Query: 243  ISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPKSG 302
            ISGVQN+++AEAMR ASNLSF S LS  RKSHLL+AK KSRL DPPAEPDRLSGL+ KSG
Sbjct: 246  ISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKSG 305

Query: 303  QLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYLR 362
            QLRSGFLG+ DD++DDPFL+ED PDDF+RG  +ALTVLQW+SL LITAALVCTLSI +LR
Sbjct: 306  QLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHLR 365

Query: 363  EKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQN 422
            E SLWE  IWKWEVMVL+LICGRLVSGWGI I VFFIERNFLLRK+VLYFVYGV++PVQN
Sbjct: 366  ENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQN 425

Query: 423  CLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSFH 482
            CLWLGLVL+AWHLLF+KRV+ QT + IL+YVR+ LVCLL+ TLIWL KTLM+KVLASSFH
Sbjct: 426  CLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVLASSFH 485

Query: 483  VSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKAA 542
            VSTYFDRIQESLFNQYVIETLSGPP IEL+K+EEE+++IADEVQ LQ+AG+ +PPDL+AA
Sbjct: 486  VSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRAA 545

Query: 543  TFASVKSGRLIGSGRTHKRFCT-KSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMKR 602
              +S+KSG  + S    +R    K  K S+   KNG++GI IDHLHKL+ KNVSAWNMKR
Sbjct: 546  ALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMKR 605

Query: 603  LLNIVRYGSISTLDEQIPGPCVDNES-TTEIKK-------LGGLSREISEGTRKYIYLDD 662
            LL IVRYGSI+TLDEQI GP +D+ES TTEIK           + + ++    KYIYLDD
Sbjct: 606  LLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKYIYLDD 665

Query: 663  LMRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNK 722
            LMRFM+E+EV KT+S FEGA E RRISKSALKNWV                         
Sbjct: 666  LMRFMKEEEVLKTLSRFEGATETRRISKSALKNWV------------------------- 725

Query: 723  LMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKT 782
                                                     VNAFRERRAL+LTLNDTKT
Sbjct: 726  -----------------------------------------VNAFRERRALSLTLNDTKT 785

Query: 783  AVDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIF 842
            AV++L  +VNVIF I IL+LW+I+LGIA+ K  +++SSQIV+VAFIFGNT K IFEA+IF
Sbjct: 786  AVERLGLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIF 845

Query: 843  LFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRY 902
            LFVMHPFDVGDRCEID +Q                         MVVEEMNILTTVFLR+
Sbjct: 846  LFVMHPFDVGDRCEIDDIQ-------------------------MVVEEMNILTTVFLRH 905

Query: 903  DNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEH 962
            DNLKII+PNSVLATK+IHN YRSPDMGE +E  VHI TP EKITAMKQRIIS+IE  KEH
Sbjct: 906  DNLKIIVPNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISFIEADKEH 932

Query: 963  WYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 1009
            W PSP+I+ KDID  + L +++WLSH MNHQD  ERWARRSV+VEEV+K+CQE DI   L
Sbjct: 966  WCPSPIILMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDITPTL 932

BLAST of Lsi11G012240 vs. NCBI nr
Match: XP_038887578.1 (mechanosensitive ion channel protein 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1608.2 bits (4163), Expect = 0.0e+00
Identity = 853/1041 (81.94%), Postives = 887/1041 (85.21%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEP DH RRLRDRH VNDSD SD 
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPADHRRRLRDRHPVNDSDSSDG 63

Query: 61   TEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSDS 120
            TEVILKIDDGGSS VSRS D  GSNGGKVWRESRY FWNN+AIGIGESASRVSGAR SDS
Sbjct: 64   TEVILKIDDGGSSTVSRSLDSVGSNGGKVWRESRYGFWNNNAIGIGESASRVSGARMSDS 123

Query: 121  GVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELRP-----PLAE 180
            GVDRNEGFEF QPGYGMEDPPTKLIGEFLHKQK+RGETTLDMDLEMEEL+P     PL E
Sbjct: 124  GVDRNEGFEFVQPGYGMEDPPTKLIGEFLHKQKIRGETTLDMDLEMEELKPDRSMTPLPE 183

Query: 181  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYK-GQPPWQQSHHERLGSP 240
            SPL+QTSKDLKVSFQQDSTEISSNDQS+RRR RDS DL EE+K GQPPWQQSHHERLGSP
Sbjct: 184  SPLNQTSKDLKVSFQQDSTEISSNDQSVRRRCRDSRDLPEEFKGGQPPWQQSHHERLGSP 243

Query: 241  TISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
            TISGVQNE+ AEAMR ASNLSFHSELSFQRKS+LLRAKTKSRLID PAEPDRLSGLIPKS
Sbjct: 244  TISGVQNESSAEAMRSASNLSFHSELSFQRKSNLLRAKTKSRLIDSPAEPDRLSGLIPKS 303

Query: 301  GQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYL 360
            GQLRSGFLGKIDDDDDDPFL+EDLPDDFKRGNFNALTVLQW+SLILITAALVCTLSIPYL
Sbjct: 304  GQLRSGFLGKIDDDDDDPFLEEDLPDDFKRGNFNALTVLQWISLILITAALVCTLSIPYL 363

Query: 361  REKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQ 420
            REKSLWELDIWKWEVM+LILICGRLVSGWGI+IVVFFIERNFLLRK+VLYFVYGVRKPVQ
Sbjct: 364  REKSLWELDIWKWEVMILILICGRLVSGWGIKIVVFFIERNFLLRKKVLYFVYGVRKPVQ 423

Query: 421  NCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSF 480
            NCLWLGLVL+AWHLLFNKRVEKQT  SILNYVRRVLVCLLIST +WLVKTLMVKVLASSF
Sbjct: 424  NCLWLGLVLIAWHLLFNKRVEKQTNASILNYVRRVLVCLLISTFLWLVKTLMVKVLASSF 483

Query: 481  HVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKA 540
            HVSTYFDRIQESLFNQYVIETLSGPPLIE+ KNEEEEER+ADEVQ LQNAGINIP DLKA
Sbjct: 484  HVSTYFDRIQESLFNQYVIETLSGPPLIEILKNEEEEERVADEVQKLQNAGINIPSDLKA 543

Query: 541  ATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMKR 600
            ATF+SVKSGR+IGSGRTHK FC KSSK S+AL KNGNDGITIDHLHKLSPKNVSAWNMKR
Sbjct: 544  ATFSSVKSGRVIGSGRTHKSFCAKSSKLSRALTKNGNDGITIDHLHKLSPKNVSAWNMKR 603

Query: 601  LLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDDL 660
            LLNIVRYGSISTLDEQI GP  D+ESTTEIK           + + ++    KYIYLDDL
Sbjct: 604  LLNIVRYGSISTLDEQIQGPGRDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDL 663

Query: 661  MRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKL 720
            MRFMREDEVFKTMSLFEGA ENRRISKSALKNWV                          
Sbjct: 664  MRFMREDEVFKTMSLFEGAAENRRISKSALKNWV-------------------------- 723

Query: 721  MLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTA 780
                                                    VNAFRERRALALTLNDTKTA
Sbjct: 724  ----------------------------------------VNAFRERRALALTLNDTKTA 783

Query: 781  VDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFL 840
            VDKLHHMVNVIF ILILILWLIVLGIA+SKFFVF+SSQIVVVAFIFGNTCKTIFEA+IFL
Sbjct: 784  VDKLHHMVNVIFSILILILWLIVLGIASSKFFVFLSSQIVVVAFIFGNTCKTIFEAIIFL 843

Query: 841  FVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYD 900
            FVMHPFDVGDRCEIDG                         T+MVVEEMNILTTVFLRYD
Sbjct: 844  FVMHPFDVGDRCEIDG-------------------------TQMVVEEMNILTTVFLRYD 903

Query: 901  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHW 960
            NLKIIIPNSVLATKLIHNFYRSP+MGESVEF VHIATPAEKITAMKQRIISYIEG+KEHW
Sbjct: 904  NLKIIIPNSVLATKLIHNFYRSPEMGESVEFYVHIATPAEKITAMKQRIISYIEGNKEHW 953

Query: 961  YPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLL 1020
             PSPM+VF DID LN LKLAVWLSHRMNHQD+GERWARRSVLVEEVVKVCQELDIQYRLL
Sbjct: 964  CPSPMVVFMDIDDLNKLKLAVWLSHRMNHQDAGERWARRSVLVEEVVKVCQELDIQYRLL 953

Query: 1021 PIDINIRSLPSSAPSIGFPSN 1029
            PIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 PIDINIRSLPSSAPSIGFPSN 953

BLAST of Lsi11G012240 vs. NCBI nr
Match: XP_008448113.1 (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1585.9 bits (4105), Expect = 0.0e+00
Identity = 840/1041 (80.69%), Postives = 876/1041 (84.15%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGG  SEINHEELPILLNH+  DH       H VNDSDPSDR
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDPSDR 63

Query: 61   TEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSDS 120
            TEVILKIDDGGSSAVSRS D   +NGGKVWRESRYDFWNND  GIGESASRV GAR SDS
Sbjct: 64   TEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDS 123

Query: 121  GVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLAE 180
            G D NEGF+F Q GYGMEDPPTKLIG+FLHKQK+RGETTLDMDLEMEEL+     PPLAE
Sbjct: 124  GDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAE 183

Query: 181  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKG-QPPWQQSHHERLGSP 240
            SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDL+EE+KG QPPWQQSHHERLGSP
Sbjct: 184  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSP 243

Query: 241  TISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
            TISGVQNE+LAEAMRCASNLSFHSELSFQRKS+LLRAKTKSRL DPPAEPDRLSGLIPKS
Sbjct: 244  TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKS 303

Query: 301  GQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYL 360
            GQLRSGFLGKI+DDDDDPFL++DLPDDFKRGNF ALTVLQW SLILITAA +CTLS+PYL
Sbjct: 304  GQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYL 363

Query: 361  REKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQ 420
            REKSLWELDIWKWEVM+LILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPVQ
Sbjct: 364  REKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQ 423

Query: 421  NCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSF 480
            NCLWLGLVL+AWHLLFNKRVEKQT TSILNYV RVLVCLLISTLIWLVKTLMVKVLASSF
Sbjct: 424  NCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSF 483

Query: 481  HVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKA 540
            HVSTYFDRIQESLFNQYVIETLSGPPL+E+RKNEEEEERIADEVQ LQNAG+ IPPDLK 
Sbjct: 484  HVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLK- 543

Query: 541  ATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMKR 600
            ATFAS+KSGR I S RTHK FC KSSKFS+AL KNGNDGITIDHLHKLSPKNVSAWNMKR
Sbjct: 544  ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKR 603

Query: 601  LLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDDL 660
            LLNIVRYGSISTLDEQI GPC+D+ESTTEIK           + + ++    KYIYLDDL
Sbjct: 604  LLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDL 663

Query: 661  MRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKL 720
            MRFMREDEV KT SLFEGA ENRRISKS LKNWV                          
Sbjct: 664  MRFMREDEVLKTTSLFEGAAENRRISKSVLKNWV-------------------------- 723

Query: 721  MLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTA 780
                                                    VN FRERRALALTLNDTKTA
Sbjct: 724  ----------------------------------------VNVFRERRALALTLNDTKTA 783

Query: 781  VDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFL 840
            VDKLHHMVNVIFGILILILWLIVLGIA+SKFF+F+SSQIVVVAFIFGNTCKTIFEA+IFL
Sbjct: 784  VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 843

Query: 841  FVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYD 900
            FVMHPFDVGDRCEIDG                         T+MVVEEMNILTTVFLRYD
Sbjct: 844  FVMHPFDVGDRCEIDG-------------------------TQMVVEEMNILTTVFLRYD 903

Query: 901  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHW 960
            NLKIIIPNSVLATKLIHNFYRSPDMGESVEF VHIATPAEKITAMKQRIISYIEG+KEHW
Sbjct: 904  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHW 945

Query: 961  YPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLL 1020
             P+PMIVFKDIDGLN LKLAVWLSHRMNHQDS ERWARRSVLVEEVVKVCQELDIQYRLL
Sbjct: 964  CPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLL 945

Query: 1021 PIDINIRSLPSSAPSIGFPSN 1029
            PIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 PIDINIRSLPSSAPSIGFPSN 945

BLAST of Lsi11G012240 vs. NCBI nr
Match: KAA0040324.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa] >TYK23381.1 mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1583.5 bits (4099), Expect = 0.0e+00
Identity = 839/1041 (80.60%), Postives = 875/1041 (84.05%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGG  SEINHEELPILLNH+  DH       H VNDSDPSDR
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDPSDR 63

Query: 61   TEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSDS 120
            TEVILKIDD GSSAVSRS D   +NGGKVWRESRYDFWNND  GIGESASRV GAR SDS
Sbjct: 64   TEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARMSDS 123

Query: 121  GVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLAE 180
            G D NEGF+F Q GYGMEDPPTKLIG+FLHKQK+RGETTLDMDLEMEEL+     PPLAE
Sbjct: 124  GDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIPPLAE 183

Query: 181  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKG-QPPWQQSHHERLGSP 240
            SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDL+EE+KG QPPWQQSHHERLGSP
Sbjct: 184  SPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHERLGSP 243

Query: 241  TISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPKS 300
            TISGVQNE+LAEAMRCASNLSFHSELSFQRKS+LLRAKTKSRL DPPAEPDRLSGLIPKS
Sbjct: 244  TISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGLIPKS 303

Query: 301  GQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYL 360
            GQLRSGFLGKI+DDDDDPFL++DLPDDFKRGNF ALTVLQW SLILITAA +CTLS+PYL
Sbjct: 304  GQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLSVPYL 363

Query: 361  REKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQ 420
            REKSLWELDIWKWEVM+LILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPVQ
Sbjct: 364  REKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVRKPVQ 423

Query: 421  NCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSF 480
            NCLWLGLVL+AWHLLFNKRVEKQT TSILNYV RVLVCLLISTLIWLVKTLMVKVLASSF
Sbjct: 424  NCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSF 483

Query: 481  HVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKA 540
            HVSTYFDRIQESLFNQYVIETLSGPPL+E+RKNEEEEERIADEVQ LQNAG+ IPPDLK 
Sbjct: 484  HVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPPDLK- 543

Query: 541  ATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMKR 600
            ATFAS+KSGR I S RTHK FC KSSKFS+AL KNGNDGITIDHLHKLSPKNVSAWNMKR
Sbjct: 544  ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAWNMKR 603

Query: 601  LLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDDL 660
            LLNIVRYGSISTLDEQI GPC+D+ESTTEIK           + + ++    KYIYLDDL
Sbjct: 604  LLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDL 663

Query: 661  MRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKL 720
            MRFMREDEV KT SLFEGA ENRRISKS LKNWV                          
Sbjct: 664  MRFMREDEVLKTTSLFEGAAENRRISKSVLKNWV-------------------------- 723

Query: 721  MLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTA 780
                                                    VN FRERRALALTLNDTKTA
Sbjct: 724  ----------------------------------------VNVFRERRALALTLNDTKTA 783

Query: 781  VDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFL 840
            VDKLHHMVNVIFGILILILWLIVLGIA+SKFF+F+SSQIVVVAFIFGNTCKTIFEA+IFL
Sbjct: 784  VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 843

Query: 841  FVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYD 900
            FVMHPFDVGDRCEIDG                         T+MVVEEMNILTTVFLRYD
Sbjct: 844  FVMHPFDVGDRCEIDG-------------------------TQMVVEEMNILTTVFLRYD 903

Query: 901  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHW 960
            NLKIIIPNSVLATKLIHNFYRSPDMGESVEF VHIATPAEKITAMKQRIISYIEG+KEHW
Sbjct: 904  NLKIIIPNSVLATKLIHNFYRSPDMGESVEFFVHIATPAEKITAMKQRIISYIEGNKEHW 945

Query: 961  YPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLL 1020
             P+PMIVFKDIDGLN LKLAVWLSHRMNHQDS ERWARRSVLVEEVVKVCQELDIQYRLL
Sbjct: 964  CPAPMIVFKDIDGLNKLKLAVWLSHRMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLL 945

Query: 1021 PIDINIRSLPSSAPSIGFPSN 1029
            PIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 PIDINIRSLPSSAPSIGFPSN 945

BLAST of Lsi11G012240 vs. NCBI nr
Match: XP_004144925.1 (mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus] >KGN43281.1 hypothetical protein Csa_020565 [Cucumis sativus])

HSP 1 Score: 1560.8 bits (4040), Expect = 0.0e+00
Identity = 833/1042 (79.94%), Postives = 876/1042 (84.07%), Query Frame = 0

Query: 1    MAKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDR 60
            + KSFKGNVSFKHTRKISAGGAGSEINHEELPILLNH+  DH R LRDRH VNDSD SDR
Sbjct: 4    LKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDTSDR 63

Query: 61   TEVILKIDDGG-SSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGARTSD 120
            TEVILKID GG SSAVSRS D AG+NGG VWRESRYDFWNND IGIGESA         +
Sbjct: 64   TEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESA-----RMGDN 123

Query: 121  SGVDRNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR-----PPLA 180
            SGVDRNEGFEF Q GYGMEDPPTKLIG+FL KQK+ GETTLDMDLEMEEL+     PPLA
Sbjct: 124  SGVDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLA 183

Query: 181  ESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYK-GQPPWQQSHHERLGS 240
            ESPLSQTSKDLKVSFQ DSTEISSNDQS+RRR RDS+DL+EE K GQ P QQ HHERLGS
Sbjct: 184  ESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGS 243

Query: 241  PTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSGLIPK 300
            PTISGVQNE+LAEAMRCASNLSFHSELSFQRKS+LLRAKTKSRLIDPPAEPDRLSGLIPK
Sbjct: 244  PTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPK 303

Query: 301  SGQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTLSIPY 360
            SGQLRSGFLGKI+DDDDDPFL++DLPD+FKRGNF+ALTVLQWVSLI+ITAAL+CTLS+PY
Sbjct: 304  SGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPY 363

Query: 361  LREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPV 420
            LREKSLWELDIWKWEVM+ ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVRKPV
Sbjct: 364  LREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPV 423

Query: 421  QNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASS 480
            QNCLWLGLVL+AWHLLFNKRVEKQT TSILNYV RVLVCLLISTLIWLVKTLMVKVLASS
Sbjct: 424  QNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASS 483

Query: 481  FHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIPPDLK 540
            FHVSTYFDRIQESLFNQYVIETLSGPPL+E+RKNEEEEERIADEVQ LQNAGI IPPDLK
Sbjct: 484  FHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLK 543

Query: 541  AATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNGNDGITIDHLHKLSPKNVSAWNMK 600
            AATFAS+KSGR IGSGRTHK FC KS K S+AL KN NDGITIDHLHKLS KNVSAWNMK
Sbjct: 544  AATFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMK 603

Query: 601  RLLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRKYIYLDD 660
            RLLNIVRYGSISTLDEQI GPC+D+ESTTEIK           + + ++    KYIYLDD
Sbjct: 604  RLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDD 663

Query: 661  LMRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAIVFATNK 720
            L+RFMREDEV KTMSLFEGA EN+RISKSALKNWV                         
Sbjct: 664  LVRFMREDEVLKTMSLFEGAAENQRISKSALKNWV------------------------- 723

Query: 721  LMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKT 780
                                                     VNAFRERRALALTLNDTKT
Sbjct: 724  -----------------------------------------VNAFRERRALALTLNDTKT 783

Query: 781  AVDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIF 840
            AVDKLHHMVNVIFGILILILWLIVLGIA+SKFF+F+SSQIVVVAFIFGNTCKTIFEA+IF
Sbjct: 784  AVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIF 843

Query: 841  LFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRY 900
            LFVMHPFDVGDRCEIDGMQ                         MVVEEMNILTTVFLRY
Sbjct: 844  LFVMHPFDVGDRCEIDGMQ-------------------------MVVEEMNILTTVFLRY 903

Query: 901  DNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEH 960
            DNLK+IIPNSVLATKLIHNFYRSPDMGES+EF VHIATPAEKITAMK RIISYIEG+KEH
Sbjct: 904  DNLKVIIPNSVLATKLIHNFYRSPDMGESIEFLVHIATPAEKITAMKHRIISYIEGNKEH 949

Query: 961  WYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 1020
            WYP+PMIVFKDIDGLN +KLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL
Sbjct: 964  WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 949

Query: 1021 LPIDINIRSLPSSAPSIGFPSN 1029
            LPIDINIRSLPSSAPSIGFPSN
Sbjct: 1024 LPIDINIRSLPSSAPSIGFPSN 949

BLAST of Lsi11G012240 vs. NCBI nr
Match: XP_022135714.1 (mechanosensitive ion channel protein 6-like [Momordica charantia])

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 765/1047 (73.07%), Postives = 835/1047 (79.75%), Query Frame = 0

Query: 3    KSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPKDHHRRLRDRHAVNDSDPSDRTE 62
            KSFK NVSFKHTRKISAGG G+EI+H+ELPILL+HEP   HR  R R ++ND DPSDRTE
Sbjct: 6    KSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHRH-RGRRSMNDCDPSDRTE 65

Query: 63   VILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIG-----IGESASRVSGART 122
            VILKIDDGGS+AVSR   P   NGGKVWRE+ YDFWNND  G         ASRVSGAR 
Sbjct: 66   VILKIDDGGSAAVSR---PVEGNGGKVWRETNYDFWNNDGKGENGASRASGASRVSGARA 125

Query: 123  SDSGVDRNEGFEFAQPGYG--MEDPPTKLIGEFLHKQKMRGETTLDMDLEMEELR----- 182
            SD+G DRNEGFEF Q GYG  +EDPP KLIGEFLHKQK+RGETTLDMDLEMEEL+     
Sbjct: 126  SDNG-DRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQDRII 185

Query: 183  PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKG-QPPWQQSHHE 242
            PPLAESPLSQTSKDLKVSFQQDSTE SSND S+RRRYRDS +LQ+EYKG Q PWQQSHHE
Sbjct: 186  PPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQSHHE 245

Query: 243  RLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPAEPDRLSG 302
            R GSPTISGVQN+  AEA RC SNLSF  ELSFQR SHLLRAKTKSRL+DPP EP  LSG
Sbjct: 246  RHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPALLSG 305

Query: 303  LIPKSGQLRSGFLGKIDDDDDDPFLDEDLPDDFKRGNFNALTVLQWVSLILITAALVCTL 362
            LIPKSG LRSGFLGK +++DDDPFL+EDLP+DFKR +F+ LT+LQW+SL+LIT ALVCTL
Sbjct: 306  LIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGALVCTL 365

Query: 363  SIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGV 422
             IPYLR  SLWEL+IWKWEVMVLILICGRLVSGWGIRI+VF IERNFLLRKR+LYFVYGV
Sbjct: 366  YIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYFVYGV 425

Query: 423  RKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCLLISTLIWLVKTLMVKV 482
            RKPVQNC+WLGLVL+AW LLFNKRVE +T  ++L YVRRVLV LL+STLIWLVKTLMVKV
Sbjct: 426  RKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTLMVKV 485

Query: 483  LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEERIADEVQNLQNAGINIP 542
            LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIE+RKNEE EER+ADEVQ LQNAG++IP
Sbjct: 486  LASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAGVSIP 545

Query: 543  PDLKAATFASVKSGRLIGSGRTHKRFCTKSS--KFSQALPKNGNDGITIDHLHKLSPKNV 602
            PDLKA T +SVKSGR+IG G   K    KSS  K S+ L KNGNDGITIDHLHKLSPKNV
Sbjct: 546  PDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLSPKNV 605

Query: 603  SAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTEIKK-------LGGLSREISEGTRK 662
            SAWNMKRLLN+VRYGSISTLDEQIPGPC+++EST EI+           + + ++    K
Sbjct: 606  SAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVARRELK 665

Query: 663  YIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSALKNWVKAKLISENVPSLSSLVSAI 722
            YIY +DLMRFMREDEV +TMSLFEGA E+RRISKS+LKNWV                   
Sbjct: 666  YIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWV------------------- 725

Query: 723  VFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCVLCIFTAYVNAFRERRALALT 782
                                                           VNAFRERR+LALT
Sbjct: 726  -----------------------------------------------VNAFRERRSLALT 785

Query: 783  LNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATSKFFVFVSSQIVVVAFIFGNTCKTI 842
            LNDTKTAVD+LHHMVNVIFGILILILWLI+LGIA+SKFF FVSSQIVVVAFIFGNTCKTI
Sbjct: 786  LNDTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTI 845

Query: 843  FEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLLNLGSDYHPTEMVVEEMNILT 902
            FEA+IFLFVMHPFDVGDRCEIDGMQ                         MVVEEMNILT
Sbjct: 846  FEAIIFLFVMHPFDVGDRCEIDGMQ-------------------------MVVEEMNILT 905

Query: 903  TVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSVHIATPAEKITAMKQRIISYI 962
            TVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGESVEF +HIATPAE I  MKQRIISYI
Sbjct: 906  TVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFCIHIATPAETIATMKQRIISYI 954

Query: 963  EGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQEL 1022
            EG+K HW PSPM VFKD++ LN L+LAVWLSHRMNHQDSGERWARRSVLVE+VVKVCQEL
Sbjct: 966  EGNKAHWSPSPMFVFKDVEELNRLRLAVWLSHRMNHQDSGERWARRSVLVEQVVKVCQEL 954

Query: 1023 DIQYRLLPIDINIRSLPSSAPSIGFPS 1028
            DIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 1026 DIQYRLLPIDINVHSLPSSAPSMGFTS 954

BLAST of Lsi11G012240 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 862.1 bits (2226), Expect = 4.6e-250
Identity = 495/999 (49.55%), Postives = 631/999 (63.16%), Query Frame = 0

Query: 56   DPSDRTEVILKID--DGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDA--IGIGESASR 115
            D +DR EVI+KID  +G ++ VS      G   GK+WR+  YDFW +    +  G +A+ 
Sbjct: 4    DAADRREVIVKIDGENGNNNGVS------GETVGKIWRDGSYDFWTDGEGNLNKGHNAAA 63

Query: 116  VSGARTSDSGVD--RNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRGETTLDMDLEMEEL 175
            V   R++ +  +  ++EGFEF +     EDPPTKLIG+FLHKQ+  GE  LDMDL M+EL
Sbjct: 64   VDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDEL 123

Query: 176  R----PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQPPWQQ 235
            +     P++ESP   T +D  V  +   +  ++ND                         
Sbjct: 124  QSRGLTPVSESPRVSTKRD-PVGRRDSRSNTNNNDD------------------------ 183

Query: 236  SHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSH-LLRAKTKSRLIDPPA-- 295
                                E ++C+ N         QR S  LL+ +T+SRL DPP   
Sbjct: 184  -------------------GEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLSDPPTPQ 243

Query: 296  ----EPDRLSGLIPKSGQLRSGFLGKI-----DDDDDDPFLDEDLPDDFKRGNFNALTVL 355
                  D  SG IPKSGQ++SGF GK      ++++DDPF  EDLP+++++   +   VL
Sbjct: 244  LPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVL 303

Query: 356  QWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIRIVVFFIE 415
            +W+SLILI A  VCTL+IP LR+K LWEL +WKWE MVL+LICGRLVS W ++IVVFFIE
Sbjct: 304  EWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIE 363

Query: 416  RNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYVRRVLVCL 475
            RNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K   T  L  V ++ VCL
Sbjct: 364  RNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCL 423

Query: 476  LISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRKNEEEEER 535
            L+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPLIE++KNEEEEER
Sbjct: 424  LVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEER 483

Query: 536  IADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTKSSKFSQALPKNG--- 595
            I+ EV+  QN G                 G  I SG       T  S F   +  NG   
Sbjct: 484  ISVEVKKFQNPG-----------------GVEIQSGAQKSPMKTGKSPFLSHVLSNGGGG 543

Query: 596  ---NDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTEIK-- 655
               N GITID LHKL+PKNVSAW MKRL+NI+R GS++TLDEQ+  P +D++   +I+  
Sbjct: 544  GGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGNQIRSE 603

Query: 656  ---KLGG--LSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSALKNW 715
               KL    +   +++   K+IY +D+MRF+ +DE  KT+SLFEGA E  RISKS+LKNW
Sbjct: 604  FEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNW 663

Query: 716  VKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISSKSCV 775
            V                                                           
Sbjct: 664  V----------------------------------------------------------- 723

Query: 776  LCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATSKFFV 835
                   VNAFRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI ++KF V
Sbjct: 724  -------VNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLV 783

Query: 836  FVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTTIVLL 895
             +SSQ+VVVAFIFGN CK +FE++I+LFV+HPFDVGDRCEIDG+Q               
Sbjct: 784  VMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQ--------------- 835

Query: 896  NLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESVEFSV 955
                      MVVEEMNILTTVFLR+DN K++ PNS+L TK I N+YRSPDMG+ +EFS+
Sbjct: 844  ----------MVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSI 835

Query: 956  HIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNHQDSG 1015
            HI TPAEKI  +KQRI SYIEG K+HWYP+PMIVFKD++ LN +++AVW +HRMNHQD G
Sbjct: 904  HITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMG 835

Query: 1016 ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSS 1020
            E+WARRS LVEE+ K+C+ELDI+YRL P+DIN+R+LP+S
Sbjct: 964  EKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLPTS 835

BLAST of Lsi11G012240 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 810.4 bits (2092), Expect = 1.6e-234
Identity = 466/1004 (46.41%), Postives = 628/1004 (62.55%), Query Frame = 0

Query: 56   DPSDRTEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGA 115
            D +DR + I+ I+   S AV  +   + + GG +W+ES YDFW+ +              
Sbjct: 5    DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK------------G 64

Query: 116  RTSDSGVDRNE---GFEFAQPG------YGMEDPPTKLIGEFLHKQKMRG-ETTLDMDLE 175
            +    G D +E    F F Q G        + DPP+KLIG+FLHKQ+  G E +LD++L 
Sbjct: 65   KNDKKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELN 124

Query: 176  MEELRPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQPPWQQ 235
            M EL+      P + ++       ++  T IS +          S  ++ + K     ++
Sbjct: 125  MAELQSNTPPRPATASNTP-----RRGLTTISES----------SSPVKTKVKADAVRRR 184

Query: 236  SHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPA--- 295
             +   LG  +    +N   AE ++C S           +K  L R KTKSRL DPP    
Sbjct: 185  QNRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTH 244

Query: 296  ----EPDRLSGLIPKSGQLRSGFLGKI------------DDDDDDPFLDEDLPDDFKRGN 355
                + +  SG   +SG  +SGFLGK             +++++DPFLDEDLP++FKR  
Sbjct: 245  PAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDK 304

Query: 356  FNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIR 415
             +    L+W+SL+LI  +LVC+L+I  L+ K+ W+LD+WKWEV VL+LICGRLVS W +R
Sbjct: 305  LSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVR 364

Query: 416  IVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYV 475
            I+VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T+++ L YV
Sbjct: 365  IIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYV 424

Query: 476  RRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRK 535
             RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+E+++
Sbjct: 425  TRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQR 484

Query: 536  NEEEEERIADEVQNLQN-AGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTKSSKFSQA 595
             EEEE+++A++V++L+  AG  +PP LKA   + +K G+  G  R               
Sbjct: 485  MEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGS------------ 544

Query: 596  LPKNGND--GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTE 655
              K G D  GI ID L +++ KNVSAWNMKRL+NI+  G+ISTLD+ +     ++E  T 
Sbjct: 545  --KRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATH 604

Query: 656  IKK-------LGGLSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSA 715
            I+           +   ++E   +YIYL+D +RF+ E+E  + M+LFEGA E+ +ISKS 
Sbjct: 605  IRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSC 664

Query: 716  LKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISS 775
            LKNWV                                                       
Sbjct: 665  LKNWV------------------------------------------------------- 724

Query: 776  KSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATS 835
                       V AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIAT+
Sbjct: 725  -----------VKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 784

Query: 836  KFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTT 895
            +F + +SSQ+++VAF+FGN+CKTIFEA+IFLFVMHPFDVGDRCEIDG+Q           
Sbjct: 785  RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQ----------- 844

Query: 896  IVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESV 955
                          +VVEEMNILTTVFLRYDN KII PNSVL TK I N+YRSPDMG++V
Sbjct: 845  --------------LVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAV 863

Query: 956  EFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNH 1015
            EF VHIATP EKITA+KQRI+SY++  K++WYP+PMIVF  +D LN +K+AVWL+HRMNH
Sbjct: 905  EFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNH 863

Query: 1016 QDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSA 1021
            QD GER+ RR +L+EEV K C+ELDI+YRL P++IN+RSLP +A
Sbjct: 965  QDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTA 863

BLAST of Lsi11G012240 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 765.4 bits (1975), Expect = 5.9e-221
Identity = 447/1007 (44.39%), Postives = 623/1007 (61.87%), Query Frame = 0

Query: 51   AVNDSDPSDRTEVILKID--DGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGES 110
            AV+ +D   R + +++ID  D G S              K WRES  +FW+ND       
Sbjct: 2    AVDSTD--QRRDFVVRIDGEDNGDSE-------------KFWRESSINFWHND------K 61

Query: 111  ASRVSGARTSDSGVD--RNEGFEFAQPGYGMEDPPTKLIGEFLHKQKMRG-ETTLDMDLE 170
            +S+  G    D   D  R    +  +P     DPP+KLI +FL+KQK  G E +LDM+  
Sbjct: 62   SSKPPGGEEDDGSFDFMRRSSEKSEEP-----DPPSKLINQFLNKQKASGDEISLDMEAN 121

Query: 171  MEELR----PPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQP 230
            M EL+    PPL+ + +S ++    V+    ++  +    ++RRR       Q      P
Sbjct: 122  MPELQKNTVPPLSSTAVSGSAS--PVTAPVTASYRNGTGDAIRRR-------QNRVTLSP 181

Query: 231  PWQQSHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPP 290
              +           + G      +E ++C SN       S  R   L++ KT+SRL+DPP
Sbjct: 182  SVKDGDSSEDEENRVDG------SEVVKCTSN------RSTMRTKTLMKMKTRSRLMDPP 241

Query: 291  AE--PDRLSGLIPKSGQLRSGFLG-------------KIDDDDDDPFLDEDLPDDFKRGN 350
                PD +SG  P+SG L  GF G             K  ++++DPF +EDLP+  ++  
Sbjct: 242  TPTYPDMVSGRTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEK 301

Query: 351  FNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIR 410
                 +++W+ LILI A+L+C+L IPYLR K+LW+L +WKWEVMVL+LICGRLVS W ++
Sbjct: 302  ICVWVIIEWIFLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVK 361

Query: 411  IVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYV 470
            + V+F+E NFL RK+VLYFVYG+RKPVQNCLWLGLVL+AWH LF+K+VE++ ++++L YV
Sbjct: 362  LFVYFVESNFLWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYV 421

Query: 471  RRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRK 530
             +VL+CLL++ +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP IE+  
Sbjct: 422  TKVLICLLVAVIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI-- 481

Query: 531  NEEEEERIADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHK--RFCTKSSKFSQ 590
               EEE++A++V+  +  G  + P        S      +GSGR  K      KS   S+
Sbjct: 482  -HIEEEKVANDVKTFEIVGRKLSP--LGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSR 541

Query: 591  ALPK--NGNDGITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCV-DNEST 650
            +  K   G +GI IDHL +++ KNVSAW MK+L+N+++ G++STLDEQI      +++  
Sbjct: 542  SGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKA 601

Query: 651  TEIK-----KLGG--LSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISK 710
            T+I+     KL    + + ++E   +YIY++D MRF+ EDE  + M LFEGA E  +ISK
Sbjct: 602  TQIRSEFEAKLAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISK 661

Query: 711  SALKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKI 770
            S LKNWV                                                     
Sbjct: 662  SCLKNWV----------------------------------------------------- 721

Query: 771  SSKSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIA 830
                         VNAFRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA
Sbjct: 722  -------------VNAFRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIA 781

Query: 831  TSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFY 890
            T+KF + +SSQ+++V F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+Q         
Sbjct: 782  TTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQ--------- 841

Query: 891  TTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGE 950
                            M+VEEMNILTTVFLR+DN KI+ PNS+L TK I N+YRSPDM +
Sbjct: 842  ----------------MIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQD 865

Query: 951  SVEFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRM 1010
            ++EF VHIATP EK TA++QRI+SY++  K+HW+PSPMIVF+D+ GLN +K+A+W +H+M
Sbjct: 902  AIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKM 865

Query: 1011 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAP 1022
            NHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++SLP++ P
Sbjct: 962  NHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATP 865

BLAST of Lsi11G012240 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 745.3 bits (1923), Expect = 6.3e-215
Identity = 440/1004 (43.82%), Postives = 596/1004 (59.36%), Query Frame = 0

Query: 56   DPSDRTEVILKIDDGGSSAVSRSQDPAGSNGGKVWRESRYDFWNNDAIGIGESASRVSGA 115
            D +DR + I+ I+   S AV  +   + + GG +W+ES YDFW+ +              
Sbjct: 5    DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK------------G 64

Query: 116  RTSDSGVDRNE---GFEFAQPG------YGMEDPPTKLIGEFLHKQKMRG-ETTLDMDLE 175
            +    G D +E    F F Q G        + DPP+KLIG+FLHKQ+  G E +LD++L 
Sbjct: 65   KNDKKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELN 124

Query: 176  MEELRPPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLQEEYKGQPPWQQ 235
            M EL+      P + ++       ++  T IS +          S  ++ + K     ++
Sbjct: 125  MAELQSNTPPRPATASNTP-----RRGLTTISES----------SSPVKTKVKADAVRRR 184

Query: 236  SHHERLGSPTISGVQNETLAEAMRCASNLSFHSELSFQRKSHLLRAKTKSRLIDPPA--- 295
             +   LG  +    +N   AE ++C S           +K  L R KTKSRL DPP    
Sbjct: 185  QNRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTH 244

Query: 296  ----EPDRLSGLIPKSGQLRSGFLGKI------------DDDDDDPFLDEDLPDDFKRGN 355
                + +  SG   +SG  +SGFLGK             +++++DPFLDEDLP++FKR  
Sbjct: 245  PAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDK 304

Query: 356  FNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGRLVSGWGIR 415
             +    L+W+SL+LI  +LVC+L+I  L+ K+ W+LD+WKWEV VL+LICGRLVS W +R
Sbjct: 305  LSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVR 364

Query: 416  IVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQTKTSILNYV 475
            I+VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T+++ L   
Sbjct: 365  IIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALR-- 424

Query: 476  RRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIELRK 535
                                           TYFDRIQESLF QYVIETLSGPPL+E+++
Sbjct: 425  -------------------------------TYFDRIQESLFTQYVIETLSGPPLMEIQR 484

Query: 536  NEEEEERIADEVQNLQN-AGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTKSSKFSQA 595
             EEEE+++A++V++L+  AG  +PP LKA   + +K G+  G  R               
Sbjct: 485  MEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPGLNRIGS------------ 544

Query: 596  LPKNGND--GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVDNESTTE 655
              K G D  GI ID L +++ KNVSAWNMKRL+NI+  G+ISTLD+ +     ++E  T 
Sbjct: 545  --KRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDATH 604

Query: 656  IKK-------LGGLSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENRRISKSA 715
            I+           +   ++E   +YIYL+D +RF+ E+E  + M+LFEGA E+ +ISKS 
Sbjct: 605  IRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSC 664

Query: 716  LKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPISDKISS 775
            LKNWV                                                       
Sbjct: 665  LKNWV------------------------------------------------------- 724

Query: 776  KSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIATS 835
                         AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIAT+
Sbjct: 725  -------------AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 784

Query: 836  KFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLICFDFYTT 895
            +F + +SSQ+++VAF+FGN+CKTIFEA+IFLFVMHPFDVGDRCEIDG+Q           
Sbjct: 785  RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQ----------- 828

Query: 896  IVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGESV 955
                          +VVEEMNILTTVFLRYDN KII PNSVL TK I N+YRSPDMG++V
Sbjct: 845  --------------LVVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAV 828

Query: 956  EFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWLSHRMNH 1015
            EF VHIATP EKITA+KQRI+SY++  K++WYP+PMIVF  +D LN +K+AVWL+HRMNH
Sbjct: 905  EFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNH 828

Query: 1016 QDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSA 1021
            QD GER+ RR +L+EEV K C+ELDI+YRL P++IN+RSLP +A
Sbjct: 965  QDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRSLPPTA 828

BLAST of Lsi11G012240 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 721.1 bits (1860), Expect = 1.3e-207
Identity = 388/772 (50.26%), Postives = 523/772 (67.75%), Query Frame = 0

Query: 264  RKSHLL-RAKTKSRLIDPPAEPD-RLSGLIPKSGQLRSGFLGKIDDD---DDDPFLDEDL 323
            RK+ L+ RAK +SRLIDPP E + + S  I  S QLRSG LG+  DD   +DD   +ED+
Sbjct: 161  RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 324  PDDFKRGNFNALTVLQWVSLILITAALVCTLSIPYLREKSLWELDIWKWEVMVLILICGR 383
            P ++++   +A+T+LQW+SLI +  ALV +L +   R  +LW L +WKWEV++L+LICGR
Sbjct: 221  PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 384  LVSGWGIRIVVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLVAWHLLFNKRVEKQT 443
            LVSG GIRI+VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281  LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 444  KTSILNYVRRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 503
            ++ +L  + ++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341  QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 504  PPLIELRKNEEEEERIADEVQNLQNAGINIPPDLKAATFASVKSGRLIGSGRTHKRFCTK 563
            PP++EL + EEEE+R  DE+  +Q  G ++ P+L +A F   KSG             T 
Sbjct: 401  PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSG------------STM 460

Query: 564  SSKFSQALPKNGND-GITIDHLHKLSPKNVSAWNMKRLLNIVRYGSISTLDEQIPGPCVD 623
            + KFS  +PK G+D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461  NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 624  NESTTEIKK-------LGGLSREISEGTRKYIYLDDLMRFMREDEVFKTMSLFEGAIENR 683
            +EST +I+           + + +++   K+IYL+DLMRF+R DE  KTM LFEGA+  +
Sbjct: 521  DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 684  RISKSALKNWVKAKLISENVPSLSSLVSAIVFATNKLMLMKLCPKLSSKIVQMLSMVVPI 743
            +I+KSALKNW+                                                 
Sbjct: 581  KITKSALKNWL------------------------------------------------- 640

Query: 744  SDKISSKSCVLCIFTAYVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIV 803
                             VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+
Sbjct: 641  -----------------VNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLIL 700

Query: 804  LGIATSKFFVFVSSQIVVVAFIFGNTCKTIFEALIFLFVMHPFDVGDRCEIDGMQVMLIC 863
            L IATSK+ +F++SQ+V++AF+FGN+ KT+FE++IFLF++HP+DVGDR  ID        
Sbjct: 701  LEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLID-------- 760

Query: 864  FDFYTTIVLLNLGSDYHPTEMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSP 923
                               EMVVEEMNILTTVFLR DNLKI+ PN +L  K IHN+ RSP
Sbjct: 761  -----------------TVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSP 820

Query: 924  DMGESVEFSVHIATPAEKITAMKQRIISYIEGHKEHWYPSPMIVFKDIDGLNMLKLAVWL 983
            DMG+ V   VHI TP EKI A+KQRI SYI+   E+WYP   ++ KD++ LN++++A+WL
Sbjct: 821  DMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWL 829

Query: 984  SHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPS 1023
             H++NHQ+ GER+ RR++L+EEV+K+  ELDIQYR  P+DIN++++P+   S
Sbjct: 881  CHKINHQNMGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSS 829