Lsi11G010520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G010520
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Allene oxide synthase
Location	chr11: 18217005 .. 18218981 (+)
RNA-Seq Expression	Lsi11G010520
Synteny	Lsi11G010520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAGTTTACTATTTATTATATTGCGTTGCTAAATGTGAATGCAAAGTCCAATCAAAACCTTAATTTAAGACAAAGACACACACGTAACTTTAAACATGCTTTTTAAATATCATTCATTAAAACTCTGTTCCTATATTCTAACCTCAAACTTTCCGCTCCCATCTTCCCCTTTCTTCAAAAAAATGACTTCATCTTCTTCCTTAGAACAGCCCCTGATTCCCCTGCCTCTCAAACCCATCCCCGGCAGCTATGGCCTCCCCATTTTCGGACCCATCAAAGATCGCTACGATTACTTCTACATCCAAGGCAGAGAAAGCTTCTTTCGAACTCGAATGGCTAAATATAACTCCACTGTCTTCCGAGCCAACATGCCGCCGGGCCCCTTCGTCTCTTCCAATTCCAGAGTCATCGTCCTTCTCGACGCCCGCAGTTTTCCCATCCTCTTCGACACAACTAAAGTCGAGAAACGCAACGTTCTCGACGGAACTTACATGCCCTCCCTCGCCTTCACCGGCGGAATTCGTGCCTGCGCCTTCTTGGACCCCTCCGAAACAGAGCACTCTGTTCTCAAAGGCCACTTCCTCAAATTTCTCGCCTCTCGTCACCACCAATTCATTCCTCTATTTCGAAGCTCCGTGTCGGAGATGTTCGATAAGCTCGAAAAGGAATTGGAAAAGAACAACGTCGCGGATTTCAACCCCGTCAGTGATTCGGCCTCGTTCGATTTCGTTTTCCGATTGCTTTCTGATGGGAGCCCCGATAAGAATTTGAACTCCGAAGGACCGGGTTTGGTAGATCGATGGCTAACTTTGCAGCTCGCTCCTTTAGCCACCCTCGGTTTGCCCAAATTTTTCAGCTGCTTCGAAGATCTCGTCATCCATACCTTCCGTTTACCGTTTGCACTCGCCAGAAGCGCTTACAGGTAAAGGAATCAGAATTGAAATTAATTAACCTTCCTATATTACTAAAAACTGTGTAGAATCAGAATCAGAAACTGATTAATGTTGTATTACAGCAAACTTTACGACTCATTTTACGAGTCGTCGGGTTCATTTCTAGACGAAGCAGAGAAGCAGGGGATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTCTAGCCGGATTCAACGCATACGCCGGAATGAAAGTGTTGTTACCGATTATACTAAACTGGGTCGGATCTGCCGGCGAGGGACTTCACCGGAAACTCGCCGGCGAAATCAGAGCCGCCGTGAAGGAAGATGGAGGACTGACATTCAGCGCCTTAGAGAAGATGAGTCTGCTGAAATCCGTCGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCGAAGGCGAAACAGGACATTGTGATCGAGAGCCATGATTCTGCTTTCAAGATCAAGAAAGGCGAAATGATTTTTGGGTATCAGCCGATCGCGACGAAAGATCCGAAGGTGTTTGAGAATCCGGAGGAATTTGTCGGCGACAGGTTCATGGGAGAGAAAGGGGACAAGCTTCTGAAGTATGTTTACTGGTCTAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTGCGGCGAGGGATCTGGTCGTGCTGGTGTCCAGAGTAGTTCTGGTGGAACTTTTCCTCCGTTACGACACGTTTGCCGTCGAGGGAACTCGCTCGTCGTTGGGTTGGTCCGTTAAGGTCAAATCGTTGACCAAAGCCAAAGCCTCATCGTAGTCAACGGTTGCACCGGGTTTCTGTATGCTTGACAGCACTTGATTGTTTGTATACGTTAAAAAACAAATAAGACTACAATTAGTGATTGTACTCTTAAATTTTTAATTTAATCCATGTATTTTTAATATTAGTTTATTGTTATTTTTTTTAAAAAAAAAAACTTTATATAGTAGGTTAACATTATGATAACATATTCACATTGTTGTATTTATTTACATGATAATTATTATTATTATTTAACTTAAAAA

mRNA sequence

AAAAAGTTTACTATTTATTATATTGCGTTGCTAAATGTGAATGCAAAGTCCAATCAAAACCTTAATTTAAGACAAAGACACACACGTAACTTTAAACATGCTTTTTAAATATCATTCATTAAAACTCTGTTCCTATATTCTAACCTCAAACTTTCCGCTCCCATCTTCCCCTTTCTTCAAAAAAATGACTTCATCTTCTTCCTTAGAACAGCCCCTGATTCCCCTGCCTCTCAAACCCATCCCCGGCAGCTATGGCCTCCCCATTTTCGGACCCATCAAAGATCGCTACGATTACTTCTACATCCAAGGCAGAGAAAGCTTCTTTCGAACTCGAATGGCTAAATATAACTCCACTGTCTTCCGAGCCAACATGCCGCCGGGCCCCTTCGTCTCTTCCAATTCCAGAGTCATCGTCCTTCTCGACGCCCGCAGTTTTCCCATCCTCTTCGACACAACTAAAGTCGAGAAACGCAACGTTCTCGACGGAACTTACATGCCCTCCCTCGCCTTCACCGGCGGAATTCGTGCCTGCGCCTTCTTGGACCCCTCCGAAACAGAGCACTCTGTTCTCAAAGGCCACTTCCTCAAATTTCTCGCCTCTCGTCACCACCAATTCATTCCTCTATTTCGAAGCTCCGTGTCGGAGATGTTCGATAAGCTCGAAAAGGAATTGGAAAAGAACAACGTCGCGGATTTCAACCCCGTCAGTGATTCGGCCTCGTTCGATTTCGTTTTCCGATTGCTTTCTGATGGGAGCCCCGATAAGAATTTGAACTCCGAAGGACCGGGTTTGGTAGATCGATGGCTAACTTTGCAGCTCGCTCCTTTAGCCACCCTCGGTTTGCCCAAATTTTTCAGCTGCTTCGAAGATCTCGTCATCCATACCTTCCGTTTACCGTTTGCACTCGCCAGAAGCGCTTACAGCAAACTTTACGACTCATTTTACGAGTCGTCGGGTTCATTTCTAGACGAAGCAGAGAAGCAGGGGATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTCTAGCCGGATTCAACGCATACGCCGGAATGAAAGTGTTGTTACCGATTATACTAAACTGGGTCGGATCTGCCGGCGAGGGACTTCACCGGAAACTCGCCGGCGAAATCAGAGCCGCCGTGAAGGAAGATGGAGGACTGACATTCAGCGCCTTAGAGAAGATGAGTCTGCTGAAATCCGTCGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCGAAGGCGAAACAGGACATTGTGATCGAGAGCCATGATTCTGCTTTCAAGATCAAGAAAGGCGAAATGATTTTTGGGTATCAGCCGATCGCGACGAAAGATCCGAAGGTGTTTGAGAATCCGGAGGAATTTGTCGGCGACAGGTTCATGGGAGAGAAAGGGGACAAGCTTCTGAAGTATGTTTACTGGTCTAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTGCGGCGAGGGATCTGGTCGTGCTGGTGTCCAGAGTAGTTCTGGTGGAACTTTTCCTCCGTTACGACACGTTTGCCGTCGAGGGAACTCGCTCGTCGTTGGGTTGGTCCGTTAAGGTCAAATCGTTGACCAAAGCCAAAGCCTCATCGTAGTCAACGGTTGCACCGGGTTTCTGTATGCTTGACAGCACTTGATTGTTTGTATACGTTAAAAAACAAATAAGACTACAATTAGTGATTGTACTCTTAAATTTTTAATTTAATCCATGTATTTTTAATATTAGTTTATTGTTATTTTTTTTAAAAAAAAAAACTTTATATAGTAGGTTAACATTATGATAACATATTCACATTGTTGTATTTATTTACATGATAATTATTATTATTATTTAACTTAAAAA

Coding sequence (CDS)

ATGCTTTTTAAATATCATTCATTAAAACTCTGTTCCTATATTCTAACCTCAAACTTTCCGCTCCCATCTTCCCCTTTCTTCAAAAAAATGACTTCATCTTCTTCCTTAGAACAGCCCCTGATTCCCCTGCCTCTCAAACCCATCCCCGGCAGCTATGGCCTCCCCATTTTCGGACCCATCAAAGATCGCTACGATTACTTCTACATCCAAGGCAGAGAAAGCTTCTTTCGAACTCGAATGGCTAAATATAACTCCACTGTCTTCCGAGCCAACATGCCGCCGGGCCCCTTCGTCTCTTCCAATTCCAGAGTCATCGTCCTTCTCGACGCCCGCAGTTTTCCCATCCTCTTCGACACAACTAAAGTCGAGAAACGCAACGTTCTCGACGGAACTTACATGCCCTCCCTCGCCTTCACCGGCGGAATTCGTGCCTGCGCCTTCTTGGACCCCTCCGAAACAGAGCACTCTGTTCTCAAAGGCCACTTCCTCAAATTTCTCGCCTCTCGTCACCACCAATTCATTCCTCTATTTCGAAGCTCCGTGTCGGAGATGTTCGATAAGCTCGAAAAGGAATTGGAAAAGAACAACGTCGCGGATTTCAACCCCGTCAGTGATTCGGCCTCGTTCGATTTCGTTTTCCGATTGCTTTCTGATGGGAGCCCCGATAAGAATTTGAACTCCGAAGGACCGGGTTTGGTAGATCGATGGCTAACTTTGCAGCTCGCTCCTTTAGCCACCCTCGGTTTGCCCAAATTTTTCAGCTGCTTCGAAGATCTCGTCATCCATACCTTCCGTTTACCGTTTGCACTCGCCAGAAGCGCTTACAGCAAACTTTACGACTCATTTTACGAGTCGTCGGGTTCATTTCTAGACGAAGCAGAGAAGCAGGGGATAAACAGAGAGAAAGCATGTCATAATTTAGTGTTTCTAGCCGGATTCAACGCATACGCCGGAATGAAAGTGTTGTTACCGATTATACTAAACTGGGTCGGATCTGCCGGCGAGGGACTTCACCGGAAACTCGCCGGCGAAATCAGAGCCGCCGTGAAGGAAGATGGAGGACTGACATTCAGCGCCTTAGAGAAGATGAGTCTGCTGAAATCCGTCGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAATACGCGAAGGCGAAACAGGACATTGTGATCGAGAGCCATGATTCTGCTTTCAAGATCAAGAAAGGCGAAATGATTTTTGGGTATCAGCCGATCGCGACGAAAGATCCGAAGGTGTTTGAGAATCCGGAGGAATTTGTCGGCGACAGGTTCATGGGAGAGAAAGGGGACAAGCTTCTGAAGTATGTTTACTGGTCTAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTGCGGCGAGGGATCTGGTCGTGCTGGTGTCCAGAGTAGTTCTGGTGGAACTTTTCCTCCGTTACGACACGTTTGCCGTCGAGGGAACTCGCTCGTCGTTGGGTTGGTCCGTTAAGGTCAAATCGTTGACCAAAGCCAAAGCCTCATCGTAG

Protein sequence

MLFKYHSLKLCSYILTSNFPLPSSPFFKKMTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFRANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKAKASS

Homology

[Close]

BLAST of Lsi11G010520 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.2e-154
Identity = 260/474 (54.85%), Postives = 349/474 (73.63%), Query Frame = 0


Query: 37  EQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFRANMPPGP 96
           E  +  LP+K IPG YG+P FG IKDRYD+ Y QG + FFR+RM KY+STVFR N+PPGP
Sbjct: 17  ESSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPPGP 76

Query: 97  FVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHS 156
           F + NS+V+VL+DA S+PILFD ++V+K N  +GT+M S +F GG + C FL  S+ +H+
Sbjct: 77  FNARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPKHT 136

Query: 157 VLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLL 216
            LKG FL  L   H +FIP+F +S++ MF  LEKEL +   + FNP+ D+ SF+F+FRL 
Sbjct: 137 TLKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKGTSYFNPIGDNLSFEFLFRLF 196

Query: 217 SDGSP--DKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSA 276
            +G    D ++   GP +VD+W+ LQLAPL +LGL    +  EDLV+HTF LP+ L +  
Sbjct: 197 CEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYILVKRD 256

Query: 277 YSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAG 336
           + KLY++FY S    LDEAEK G+ R++ACHN VFLAGFN+Y G+KV  P ++ W+G++G
Sbjct: 257 HQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWIGTSG 316

Query: 337 EGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIE 396
             LH +L  EIR AVKE GG+T SA++KM L+KSVVYE LR+DPPVP+Q  KA+++I+I 
Sbjct: 317 PSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKNIIIT 376

Query: 397 SHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETE 456
           +H+S+F IKK E+IFGYQP+ATKD KVF+N EEF  DRF+G  G+KLLKYVYWSNG+E +
Sbjct: 377 NHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWSNGKEID 436

Query: 457 EPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
            P+  +KQC  +DL+VL+ R+++VE F+RYDTF VE  +  LG  V  KSLTKA
Sbjct: 437 NPSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKA 489

[Close]

BLAST of Lsi11G010520 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.5e-149
Identity = 265/491 (53.97%), Postives = 351/491 (71.49%), Query Frame = 0


Query: 20  PLPSSPFFKKMTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTR 79
           P  SSP  +  +SSSS       LP + +PG YGLP+ GP KDR DYFY QG+  FF++R
Sbjct: 46  PYISSP-SQSPSSSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSR 105

Query: 80  MAKYNSTVFRANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFT 139
           + K+ STVFR NMPPGPF+S N  V+VLLD +SFP+LFD +KVEK+++  GT+MPS   T
Sbjct: 106 IQKHQSTVFRTNMPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLT 165

Query: 140 GGIRACAFLDPSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVAD 199
           GG R  ++LDPSE  H+ LK      L+SR ++ IP F +S SE+F+ LE EL     A 
Sbjct: 166 GGYRVLSYLDPSEPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAG 225

Query: 200 FNPVSDSASFDFVFRLLSDGSP-DKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFED 259
            N  +D A+F+F+ R L   +P D  L ++GP L+ +W+  QL PL  LGLPK     ED
Sbjct: 226 LNAANDQAAFNFLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPK---VLED 285

Query: 260 LVIHTFRLPFALARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAG 319
           LV+HTFRLP AL +  Y +LY+ FYE+S S LDEAEK GI+RE+ACHNL+F   FN++ G
Sbjct: 286 LVMHTFRLPPALVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGG 345

Query: 320 MKVLLPIILNWVGSAGEGLHRKLAGEIRAAVKEDGG-LTFSALEKMSLLKSVVYEVLRID 379
           +K+  P +L W+G AG  LH +LA EIR+ +  + G +T +A+EKM L+KSVVYE LRI+
Sbjct: 346 IKIFFPNMLKWIGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIE 405

Query: 380 PPVPYQYAKAKQDIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEK 439
           PPV  QY +AK D+VIESHD++F+IK+GE+++GYQP ATKDPK+F+  EEFV DRF+GE+
Sbjct: 406 PPVASQYGRAKHDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEE 465

Query: 440 GDKLLKYVYWSNGRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLG 499
           G+KLLK+V WSNG ETE  +  NKQCA +D VVLVSR++LVELFLRYD+F +E   S LG
Sbjct: 466 GEKLLKHVLWSNGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLG 525

Query: 500 WSVKVKSLTKA 509
            ++ + SL +A
Sbjct: 526 AAITLTSLRRA 532

[Close]

BLAST of Lsi11G010520 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.0e-146
Identity = 261/511 (51.08%), Postives = 349/511 (68.30%), Query Frame = 0


Query: 15  LTSNFPLPSSPFFKKMTSSSSLEQPL---------------IPLPLKPIPGSYGLPIFGP 74
           LT +F LP     +K+ S  S  +P+               I L  + IPG YGLP  GP
Sbjct: 3   LTLSFSLPLPSLHQKIPSKYSTFRPIIVSLSDKSTIEITQPIKLSTRTIPGDYGLPGIGP 62

Query: 75  IKDRYDYFYIQGRESFFRTRMAKYNSTVFRANMPPGPFVSSNSRVIVLLDARSFPILFDT 134
            KDR DYFY QG+  FF +R+AKY ST+FR NMPPGPF++SN +VIVLLD +SFP+LFD 
Sbjct: 63  WKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSNPKVIVLLDGKSFPVLFDA 122

Query: 135 TKVEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHSVLKGHFLKFLASRHHQFIPLFRS 194
           +KVEK+++  GT++PS   TGG R  ++LDPSE  H  LK      L+SR    IP F  
Sbjct: 123 SKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKLMFFLLSSRRDHVIPEFHE 182

Query: 195 SVSEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLLSDGSP-DKNLNSEGPGLVDRWLT 254
           + +E+F+ L+KE+E+     FN  SD A+F+F+ R L   +P +  L ++GP L+ +W+ 
Sbjct: 183 TYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFGVNPVETKLGTDGPALIGKWIL 242

Query: 255 LQLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSAYSKLYDSFYESSGSFLDEAEKQGI 314
           LQL P+ TLGLPKF    +D+++HTFRLP  L +  Y +LYD FY +S +   EAEK GI
Sbjct: 243 LQLHPVITLGLPKF---LDDVLLHTFRLPPILVKKDYQRLYDFFYTNSANLFIEAEKLGI 302

Query: 315 NREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAGEGLHRKLAGEIRAAVKEDGG-LTF 374
           ++++ACHNL+F   FN++ GMK+  P +L  +  AG  +H +LA EIR+ VK  GG +T 
Sbjct: 303 SKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHTRLANEIRSEVKSAGGKITM 362

Query: 375 SALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAFKIKKGEMIFGYQPIATK 434
           SA+EKM L+KSVVYE LR+DPPV  QY +AKQD+ IESHD+ F++KKGE++FGYQP ATK
Sbjct: 363 SAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDAVFEVKKGEILFGYQPFATK 422

Query: 435 DPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPTAENKQCAARDLVVLVSRVVL 494
           DPK+F+ P EFV DRF+GE+G+KLLK+V WSNG ETE PT  NKQCA +D VV+VSR+ +
Sbjct: 423 DPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTVGNKQCAGKDFVVMVSRLFV 482

Query: 495 VELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
            E FLRY T  V+   S+LG S+ + SL KA
Sbjct: 483 TEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

[Close]

BLAST of Lsi11G010520 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 512.7 bits (1319), Expect = 4.8e-144
Identity = 249/468 (53.21%), Postives = 335/468 (71.58%), Query Frame = 0


Query: 43  LPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFRANMPPGPFVSSNS 102
           LP++ IPG+YGLPI GPIKDR+DYFY QG E FF++R+ KYNSTV+R NMPPG F++ N 
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENP 111

Query: 103 RVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHSVLKGHF 162
           +V+ LLD +SFP+LFD  KVEK+++  GTYMPS   TGG R  ++LDPSE +H  LK   
Sbjct: 112 QVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLL 171

Query: 163 LKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLLSDGSP- 222
              L S  ++  P F+++ SE+FD LEKEL     ADF   SD  +F+F+ R     +P 
Sbjct: 172 FFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPA 231

Query: 223 DKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSAYSKLYDS 282
           D  L ++ PGL+ +W+   L PL ++GLP+     E+ +IHTF LP AL +S Y +LY+ 
Sbjct: 232 DTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 283 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAGEGLHRKL 342
           F ES+G  L EA+K GI+RE+A HNL+F   FN + GMK+L P ++  +G AG  +H +L
Sbjct: 292 FLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRL 351

Query: 343 AGEIRAAVKEDGG-LTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAF 402
           A EIR+ +K +GG LT  A+EKM L KSVVYE LR +PPV  QY +AK+D+VIESHD+AF
Sbjct: 352 AEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAF 411

Query: 403 KIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPTAEN 462
           K+K GEM++GYQP+AT+DPK+F+  +EFV +RF+GE+G+KLL++V WSNG ETE PT  N
Sbjct: 412 KVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGN 471

Query: 463 KQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           KQCA +D VVLV+R+ ++E+F RYD+F +E   S LG SV   SL KA
Sbjct: 472 KQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

[Close]

BLAST of Lsi11G010520 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 506.9 bits (1304), Expect = 2.7e-142
Identity = 252/469 (53.73%), Postives = 333/469 (71.00%), Query Frame = 0


Query: 44  PLKPIPGSYGLPIFGPIKDRYDYFY-IQGRESFFRTRMAKYNSTVFRANMPPGPFVSSNS 103
           PL+ IPGSYG+P F PIKDR +YFY   GR+ +FR+RM KY STVFRANMPPGPFVSSN 
Sbjct: 7   PLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPFVSSNP 66

Query: 104 RVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHSVLKGHF 163
           +VIVLLDA+SFPILFD +KVEK+++  GTYMPS   TGG R  ++LDPSE  H+ LK   
Sbjct: 67  KVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQLKNLL 126

Query: 164 LKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLLSDGSPD 223
              L +  ++ IP F ++ +E+F+ LE EL KN  A FN V + A+F F+ R   + +P+
Sbjct: 127 FFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYFNSNPE 186

Query: 224 K-NLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSAYSKLYDS 283
           +  L +  P L+  W+   LAP   LGLP F    ++ ++HTFRLP  L +S Y+KLYD 
Sbjct: 187 ETKLGTSAPTLISSWVLFNLAPTLDLGLPWF---LQEPLLHTFRLPAFLIKSTYNKLYDY 246

Query: 284 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAGEGLHRKL 343
           F   +   +++AEK G+ +++A HN++F   FN + G+K+L P  L W+G AGE LH +L
Sbjct: 247 FQSVATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHTQL 306

Query: 344 AGEIRAAVKE--DGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSA 403
           A EIR A+K   DG +T  A+E+M L KSVVYE LRI+PPVP QY KAK +  IESHD+ 
Sbjct: 307 AEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHDAT 366

Query: 404 FKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPTAE 463
           F++KKGEM+FGYQP ATKDPKVF+ PEE+V DRF+G+ G+ LLKYV+WSNG ETE PT E
Sbjct: 367 FEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGD-GEALLKYVWWSNGPETESPTVE 426

Query: 464 NKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           NKQCA +D VVL++R+ ++ELF RYD+F +E   S LG +V +  L +A
Sbjct: 427 NKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRA 471

[Close]

BLAST of Lsi11G010520 vs. ExPASy TrEMBL
Match: A0A0A0K2U8 (Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.2e-250
Identity = 431/479 (89.98%), Postives = 454/479 (94.78%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P IPLPLKPIPGSYG PIFGPI DRY YFYIQGRE+FFR+RMAKYNSTVFR
Sbjct: 1   MTSSSS-EHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SSNS+VIVLLDA +FPILFDTTKVEKRNVLDGTYMPSLAFTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFLKFLASRHHQFIPLFR+S+SEMFDKLEKEL+ NNVA+FNP+SD ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSD SPDKN +SEGPGLVDRWLT+QLAPLATLGLPK FSCFEDL+IHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L +SAY KLY+SFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAGE LHRKL GEIRAAVK DGG+TF ALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DIVIESHDSAF+IKKGEMIFGYQPIATKDPKVFEN EEFVG+RF+GEKG+KLLKYVYWSN
Sbjct: 361 DIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVL+ RVVLVELFLRYDTF VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

[Close]

BLAST of Lsi11G010520 vs. ExPASy TrEMBL
Match: A0A5A7SKU9 (Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00100 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.4e-249
Identity = 429/479 (89.56%), Postives = 453/479 (94.57%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P IPLPLKPIPG+ G PIFGPIKDRY YFYIQGRE+FFR+RMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SSNS+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPSLAFTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFL FLASRHHQFIPLFR+S+SEMFDKLEKEL+ NNVA+FNPVSDSASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSD SP+KN  SEGPGLVDRWLT+QLAPLATLGLPK FSCFEDL+IHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L +SAY KLY+SFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAGE LHRKL  EIRAAVK+D G+TF ALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DIVIE+HDSAFKIKKGEMIFGYQPIATKDPKVFEN EEFVGDRF+GEKG+KLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVL+SRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

[Close]

BLAST of Lsi11G010520 vs. ExPASy TrEMBL
Match: A0A1S3BZQ0 (allene oxide synthase 3-like OS=Cucumis melo OX=3656 GN=LOC103495194 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.4e-249
Identity = 429/479 (89.56%), Postives = 453/479 (94.57%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P IPLPLKPIPG+ G PIFGPIKDRY YFYIQGRE+FFR+RMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SSNS+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPSLAFTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFL FLASRHHQFIPLFR+S+SEMFDKLEKEL+ NNVA+FNPVSDSASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSD SP+KN  SEGPGLVDRWLT+QLAPLATLGLPK FSCFEDL+IHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L +SAY KLY+SFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAGE LHRKL  EIRAAVK+D G+TF ALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DIVIE+HDSAFKIKKGEMIFGYQPIATKDPKVFEN EEFVGDRF+GEKG+KLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVL+SRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

[Close]

BLAST of Lsi11G010520 vs. ExPASy TrEMBL
Match: A0A6J1GLX0 (allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.4e-241
Identity = 413/478 (86.40%), Postives = 445/478 (93.10%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P  PLPLKPIPGSYGLP FGPIK RYDYFY QGRE FFR+RMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SS+SRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPS++FTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFLKFLASRH +FIPLFR+SVSEMF KLEKELEKNN ADFN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSDG PD+ L S+G  LVDRWL +QLAPLATLGLPKF SCFEDL+IHTF LPFA
Sbjct: 181 DFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L ++AY KLYD+FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW
Sbjct: 241 LVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAG+ LH+KLAGEIRAAVKE+ GLTF+A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DI+IESHDSAFKIKKGEMIFGYQPIAT+DPKVFE PEEF+GDRF+GE+G+KLLKYVYWSN
Sbjct: 361 DIIIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTK 508
           GRETEEPTAENKQC ARDLVVLVSRVVL ELFLRYDTFAVEGT+SSLGWSVKVKSLTK
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

[Close]

BLAST of Lsi11G010520 vs. ExPASy TrEMBL
Match: A0A6J1IB84 (allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 6.6e-237
Identity = 408/479 (85.18%), Postives = 439/479 (91.65%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P  P  LKPIPG YGLP FGPIK RYDYFY QGRE FFR+RMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPPSLKPIPGGYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SS+SRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPS++FTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFLKFLASRH +FIPLFR+SVSEMF KLEKELEK N ADFN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRTSVSEMFSKLEKELEKTNAADFNSVSDAASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSDG P + L S G  LVDRWL +QLAPLATLGLPKF SCFEDL+IHTF LPFA
Sbjct: 181 DFIFRLLSDGKPGEKLGSNGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L ++AY KLYD+FYESS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW
Sbjct: 241 LVKNAYRKLYDAFYESSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAG+ LHRKLAGEIRAAVKE+ GLTF+A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DI+IESHDSAF+IKKGEMIFGYQPIAT+DPKVFE PEEF+G+RF+GE+G+KLLKYVYWSN
Sbjct: 361 DIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGERFVGEEGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVLVSRVVL ELFLRYDTF VEGT+SSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFTVEGTQSSLGWSVKVKSLTKA 479

[Close]

BLAST of Lsi11G010520 vs. NCBI nr
Match: XP_038888826.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 902.5 bits (2331), Expect = 1.7e-258
Identity = 445/481 (92.52%), Postives = 467/481 (97.09%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSS S EQPLIPLPLKPIPGSYG PIFGPIKDRYDYFYIQGRE FFRTRMAKY+STVFR
Sbjct: 1   MTSSPSSEQPLIPLPLKPIPGSYGFPIFGPIKDRYDYFYIQGREKFFRTRMAKYDSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SSNS+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPSLAFTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHS+LK HFLKFLASRHHQFIPLFRSSVSEMF+KL++ELEKN VADFNPVSDSASF
Sbjct: 121 PSETEHSLLKRHFLKFLASRHHQFIPLFRSSVSEMFNKLDRELEKNKVADFNPVSDSASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DFVFRLLSD SPDK+L+S+GPGLVDRWL LQLAPLATLGLPKF SCFED++IHTFRLPFA
Sbjct: 181 DFVFRLLSDRSPDKSLSSKGPGLVDRWLALQLAPLATLGLPKFLSCFEDIIIHTFRLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           LARSAYSKLY+SFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW
Sbjct: 241 LARSAYSKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAGEGLHRKLA EIRAAVKEDGG+TFSALEKMSLLKSV+YEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGEGLHRKLAVEIRAAVKEDGGVTFSALEKMSLLKSVIYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DIVIESHDSAFKIKKGEMIFGYQPIATKDP+VFENPEEF+GDRF+GEKG+KLLKYVYWSN
Sbjct: 361 DIVIESHDSAFKIKKGEMIFGYQPIATKDPRVFENPEEFIGDRFVGEKGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKAK 509
           GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGT+SSLGWSVKVKSL+KA 
Sbjct: 421 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTQSSLGWSVKVKSLSKAS 480

Query: 510 A 511
           +
Sbjct: 481 S 481

[Close]

BLAST of Lsi11G010520 vs. NCBI nr
Match: XP_004137005.1 (allene oxide synthase 3 [Cucumis sativus] >KGN43980.1 hypothetical protein Csa_017715 [Cucumis sativus])

HSP 1 Score: 873.2 bits (2255), Expect = 1.1e-249
Identity = 431/479 (89.98%), Postives = 454/479 (94.78%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P IPLPLKPIPGSYG PIFGPI DRY YFYIQGRE+FFR+RMAKYNSTVFR
Sbjct: 1   MTSSSS-EHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SSNS+VIVLLDA +FPILFDTTKVEKRNVLDGTYMPSLAFTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFLKFLASRHHQFIPLFR+S+SEMFDKLEKEL+ NNVA+FNP+SD ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNNVANFNPISDYASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSD SPDKN +SEGPGLVDRWLT+QLAPLATLGLPK FSCFEDL+IHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L +SAY KLY+SFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAGE LHRKL GEIRAAVK DGG+TF ALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DIVIESHDSAF+IKKGEMIFGYQPIATKDPKVFEN EEFVG+RF+GEKG+KLLKYVYWSN
Sbjct: 361 DIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVL+ RVVLVELFLRYDTF VEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

[Close]

BLAST of Lsi11G010520 vs. NCBI nr
Match: XP_008454884.1 (PREDICTED: allene oxide synthase 3-like [Cucumis melo] >KAA0031258.1 allene oxide synthase 3-like [Cucumis melo var. makuwa] >TYK06709.1 allene oxide synthase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 870.2 bits (2247), Expect = 9.1e-249
Identity = 429/479 (89.56%), Postives = 453/479 (94.57%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P IPLPLKPIPG+ G PIFGPIKDRY YFYIQGRE+FFR+RMAKYNSTVFR
Sbjct: 1   MTSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SSNS+VIVLLDA SFPILFDTTKVEKRNVLDGTYMPSLAFTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFL FLASRHHQFIPLFR+S+SEMFDKLEKEL+ NNVA+FNPVSDSASF
Sbjct: 121 PSETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKELKNNNVANFNPVSDSASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSD SP+KN  SEGPGLVDRWLT+QLAPLATLGLPK FSCFEDL+IHTFRLPFA
Sbjct: 181 DFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L +SAY KLY+SFYESSG+FLDEAEKQGINREKACHNLVFLAGFNAYAGMKVL PI+LNW
Sbjct: 241 LVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAGE LHRKL  EIRAAVK+D G+TF ALEKMSLLKSVVYEVLRIDPPVPYQYA+AKQ
Sbjct: 301 VGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DIVIE+HDSAFKIKKGEMIFGYQPIATKDPKVFEN EEFVGDRF+GEKG+KLLKYVYWSN
Sbjct: 361 DIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVL+SRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

[Close]

BLAST of Lsi11G010520 vs. NCBI nr
Match: XP_022952977.1 (allene oxide synthase 3-like [Cucurbita moschata])

HSP 1 Score: 843.6 bits (2178), Expect = 9.1e-241
Identity = 413/478 (86.40%), Postives = 445/478 (93.10%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P  PLPLKPIPGSYGLP FGPIK RYDYFY QGRE FFR+RMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SS+SRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPS++FTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFLKFLASRH +FIPLFR+SVSEMF KLEKELEKNN ADFN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSDG PD+ L S+G  LVDRWL +QLAPLATLGLPKF SCFEDL+IHTF LPFA
Sbjct: 181 DFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
           L ++AY KLYD+FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW
Sbjct: 241 LVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAG+ LH+KLAGEIRAAVKE+ GLTF+A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DI+IESHDSAFKIKKGEMIFGYQPIAT+DPKVFE PEEF+GDRF+GE+G+KLLKYVYWSN
Sbjct: 361 DIIIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTK 508
           GRETEEPTAENKQC ARDLVVLVSRVVL ELFLRYDTFAVEGT+SSLGWSVKVKSLTK
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

[Close]

BLAST of Lsi11G010520 vs. NCBI nr
Match: KAG6572010.1 (Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 843.2 bits (2177), Expect = 1.2e-240
Identity = 413/479 (86.22%), Postives = 445/479 (92.90%), Query Frame = 0


Query: 30  MTSSSSLEQPLIPLPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFR 89
           MTSSSS E P  PLPLKPIPGSYGLP FGPIK RYDYFY QGRE FFR+RMA+Y STVFR
Sbjct: 1   MTSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFR 60

Query: 90  ANMPPGPFVSSNSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLD 149
            NMPPGPF+SS+SRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPS++FTGGIR CAFLD
Sbjct: 61  TNMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLD 120

Query: 150 PSETEHSVLKGHFLKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASF 209
           PSETEHSVLK HFLKFLASRH +FIPLFR+SVSEMF KLEKELEKNN ADFN VSD+ASF
Sbjct: 121 PSETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNNAADFNSVSDAASF 180

Query: 210 DFVFRLLSDGSPDKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFA 269
           DF+FRLLSDG PD+ L S+G  LVDRWL +QLAPLATLGLPKF SCFEDL+IHTF LPFA
Sbjct: 181 DFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPFA 240

Query: 270 LARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 329
             ++AY KLYD+FY+SS +FL+EAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW
Sbjct: 241 FVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNW 300

Query: 330 VGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQ 389
           VGSAG+ LHRKLAGEIRAAVKE+ GLTF+A+E MSLLKSVVYEVLRIDPPVPYQYAKAKQ
Sbjct: 301 VGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAKQ 360

Query: 390 DIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSN 449
           DI+IESHDSAF+IKKGEMIFGYQPIAT+DPKVFE PEEF+GDRF+GE+G+KLLKYVYWSN
Sbjct: 361 DIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWSN 420

Query: 450 GRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           GRETEEPTAENKQC ARDLVVLVSRVVL ELFLRYDTFAVEGT+SSLGWSVKVKSLTKA
Sbjct: 421 GRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTKA 479

[Close]

BLAST of Lsi11G010520 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 512.7 bits (1319), Expect = 3.4e-145
Identity = 249/468 (53.21%), Postives = 335/468 (71.58%), Query Frame = 0


Query: 43  LPLKPIPGSYGLPIFGPIKDRYDYFYIQGRESFFRTRMAKYNSTVFRANMPPGPFVSSNS 102
           LP++ IPG+YGLPI GPIKDR+DYFY QG E FF++R+ KYNSTV+R NMPPG F++ N 
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENP 111

Query: 103 RVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHSVLKGHF 162
           +V+ LLD +SFP+LFD  KVEK+++  GTYMPS   TGG R  ++LDPSE +H  LK   
Sbjct: 112 QVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLL 171

Query: 163 LKFLASRHHQFIPLFRSSVSEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLLSDGSP- 222
              L S  ++  P F+++ SE+FD LEKEL     ADF   SD  +F+F+ R     +P 
Sbjct: 172 FFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPA 231

Query: 223 DKNLNSEGPGLVDRWLTLQLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSAYSKLYDS 282
           D  L ++ PGL+ +W+   L PL ++GLP+     E+ +IHTF LP AL +S Y +LY+ 
Sbjct: 232 DTKLKADAPGLITKWVLFNLHPLLSIGLPR---VIEEPLIHTFSLPPALVKSDYQRLYEF 291

Query: 283 FYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAGEGLHRKL 342
           F ES+G  L EA+K GI+RE+A HNL+F   FN + GMK+L P ++  +G AG  +H +L
Sbjct: 292 FLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRL 351

Query: 343 AGEIRAAVKEDGG-LTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAF 402
           A EIR+ +K +GG LT  A+EKM L KSVVYE LR +PPV  QY +AK+D+VIESHD+AF
Sbjct: 352 AEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAF 411

Query: 403 KIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPTAEN 462
           K+K GEM++GYQP+AT+DPK+F+  +EFV +RF+GE+G+KLL++V WSNG ETE PT  N
Sbjct: 412 KVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGN 471

Query: 463 KQCAARDLVVLVSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 509
           KQCA +D VVLV+R+ ++E+F RYD+F +E   S LG SV   SL KA
Sbjct: 472 KQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKA 516

[Close]

BLAST of Lsi11G010520 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 289.3 bits (739), Expect = 6.2e-78
Identity = 150/391 (38.36%), Postives = 231/391 (59.08%), Query Frame = 0


Query: 122 VEKRNVLDGTYMPSLAFTGGIRACAFLDPSETEHSVLKGHFLKFLASRHHQFIPLFRSSV 181
           V+KR+VL G + PSL F GG+R   +LD +E +H+ +KG  ++ L      ++   RS++
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 182 SEMFDKLEKELEKNNVADFNPVSDSASFDFVFRLLS--DGSPDKNLNSEGPGLVDRWLTL 241
           +  +  +E E+ KN  A +        F F+   L+  D S   ++   G   ++ WL L
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 242 QLAPLATLGLPKFFSCFEDLVIHTFRLPFALARSAYSKLYDSFYESSGSFLDEAEKQ-GI 301
           Q+ P A LG+       E++++HT+  P  L    Y KLY+   E++G  L   +++ G+
Sbjct: 124 QVIPTAKLGVVP--QPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFGL 183

Query: 302 NREKACHNLVFLAGFNAYAGMKVLLPIILNWVGSAGEGLHRKLAGEIRAAVKEDGGLTFS 361
            R++A  NL+F+ GFNAY G  V LP ++  +     GL  ++  E+R        L F 
Sbjct: 184 TRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNFK 243

Query: 362 ALEKMSLLKSVVYEVLRIDPPVPYQYAKAKQDIVIESHDSAFKIKKGEMIFGYQPIATKD 421
            + +M L+KSVVYE LR  PPVP Q+A+A++D  I SHD+ F++KKGE++ GYQP+  +D
Sbjct: 244 TVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRD 303

Query: 422 PKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPTAENKQCAARDLVVLVSRVVLV 481
             VF+ PEEF  DR++GE G +LL Y+YWSNG +T  P+A NKQCAA+D+V L + +++ 
Sbjct: 304 ANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVA 363

Query: 482 ELFLRYDTFAVEGTRSSLGWSVKVKSLTKAK 510
           +LFLRYDT          G S  +K++ KAK
Sbjct: 364 DLFLRYDTIT--------GDSGSIKAVVKAK 384

[Close]

BLAST of Lsi11G010520 vs. TAIR 10
Match: AT3G20120.1 (cytochrome P450, family 705, subfamily A, polypeptide 21 )

HSP 1 Score: 53.5 bits (127), Expect = 5.7e-07
Identity = 64/252 (25.40%), Postives = 108/252 (42.86%), Query Frame = 0


Query: 223 KNLNSEGPGLVDRWL--TLQLAPLATLGLPKF---FSCFED---------LVIHTFRLPF 282
           + L +E  GL  + L  TL   PL  LG+  F     C  D         LV H  +L  
Sbjct: 77  RGLVTELDGLTKKVLFATLLHRPLEKLGISLFKKEIMCVSDSFDEVLERVLVEHEQKLDD 136

Query: 283 ALARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYA-GMKVLLPIIL 342
              R     L  ++ + +        K   N  KA    +F AG +  A  ++  +  I+
Sbjct: 137 HQDRDMMDVLLAAYGDENAE-----HKISRNHIKAFFVELFFAGTDTSAQSIQWTMAEII 196

Query: 343 NWVGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKA 402
           N        + ++L  EI + V +   +  + L K+  L++VV E LR+ PP+P      
Sbjct: 197 N-----NPNILKRLREEIDSVVGKTRLIQETDLPKLPYLQAVVKEGLRLHPPLPLFVRTF 256

Query: 403 KQDIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMG--------EKGD 452
           ++   I      F + +   + G   +  +DP V+E+PEEF  +RF+         E+ +
Sbjct: 257 QEGCKI----GGFYVPEKTTLIGNAYVMMRDPSVWEDPEEFKPERFLSSSRSTQEEERRE 314

[Close]

BLAST of Lsi11G010520 vs. TAIR 10
Match: AT3G20120.2 (cytochrome P450, family 705, subfamily A, polypeptide 21 )

HSP 1 Score: 53.5 bits (127), Expect = 5.7e-07
Identity = 64/252 (25.40%), Postives = 108/252 (42.86%), Query Frame = 0


Query: 223 KNLNSEGPGLVDRWL--TLQLAPLATLGLPKF---FSCFED---------LVIHTFRLPF 282
           + L +E  GL  + L  TL   PL  LG+  F     C  D         LV H  +L  
Sbjct: 77  RGLVTELDGLTKKVLFATLLHRPLEKLGISLFKKEIMCVSDSFDEVLERVLVEHEQKLDD 136

Query: 283 ALARSAYSKLYDSFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYA-GMKVLLPIIL 342
              R     L  ++ + +        K   N  KA    +F AG +  A  ++  +  I+
Sbjct: 137 HQDRDMMDVLLAAYGDENAE-----HKISRNHIKAFFVELFFAGTDTSAQSIQWTMAEII 196

Query: 343 NWVGSAGEGLHRKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAKA 402
           N        + ++L  EI + V +   +  + L K+  L++VV E LR+ PP+P      
Sbjct: 197 N-----NPNILKRLREEIDSVVGKTRLIQETDLPKLPYLQAVVKEGLRLHPPLPLFVRTF 256

Query: 403 KQDIVIESHDSAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMG--------EKGD 452
           ++   I      F + +   + G   +  +DP V+E+PEEF  +RF+         E+ +
Sbjct: 257 QEGCKI----GGFYVPEKTTLIGNAYVMMRDPSVWEDPEEFKPERFLSSSRSTQEEERRE 314

[Close]

BLAST of Lsi11G010520 vs. TAIR 10
Match: AT5G25180.1 (cytochrome P450, family 71, subfamily B, polypeptide 14 )

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-06
Identity = 42/154 (27.27%), Postives = 70/154 (45.45%), Query Frame = 0


Query: 339 RKLAGEIRAAVKEDGGLTFSALEKMSLLKSVVYEVLRIDPPVPYQYAK-AKQDIVIESHD 398
           +K   E+R  +K    +T   +E++  LK V+ E LRI+P VP    + A + I I  +D
Sbjct: 321 KKAQAEVREVIKNKDDITEEDIERLEYLKMVIKETLRINPLVPLLIPREASKYIKIGGYD 380

Query: 399 SAFKIKKGEMIFGYQPIATKDPKVFENPEEFVGDRFMGEKGDKLLKYVYWSNGRETEEPT 458
               I K   I+       ++P V+++PE F+ +RFM  + D      Y     E     
Sbjct: 381 ----IPKKTWIYVNIWAVQRNPNVWKDPEVFIPERFMHSEID------YKGVDFELLPFG 440

Query: 459 AENKQCAARDLVVLVSRVVLVELFLRYDTFAVEG 492
           +  + C    L + +  + L+ L  R+D    EG
Sbjct: 441 SGRRMCPGMGLGMALVHLTLINLLYRFDWKLPEG 464

The following BLAST results are available for this feature:

BLAST of Lsi11G010520 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GZP5	5.2e-154	54.85	Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1	[more]
K4BV52	6.5e-149	53.97	Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P...	[more]
Q9LLB0	1.0e-146	51.08	Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P...	[more]
Q96242	4.8e-144	53.21	Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P...	[more]
Q40778	2.7e-142	53.73	Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2	[more]

BLAST of Lsi11G010520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2U8	5.2e-250	89.98	Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4...	[more]
A0A5A7SKU9	4.4e-249	89.56	Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BZQ0	4.4e-249	89.56	allene oxide synthase 3-like OS=Cucumis melo OX=3656 GN=LOC103495194 PE=4 SV=1	[more]
A0A6J1GLX0	4.4e-241	86.40	allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 ...	[more]
A0A6J1IB84	6.6e-237	85.18	allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV...	[more]

BLAST of Lsi11G010520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888826.1	1.7e-258	92.52	allene oxide synthase 3-like [Benincasa hispida]	[more]
XP_004137005.1	1.1e-249	89.98	allene oxide synthase 3 [Cucumis sativus] >KGN43980.1 hypothetical protein Csa_0...	[more]
XP_008454884.1	9.1e-249	89.56	PREDICTED: allene oxide synthase 3-like [Cucumis melo] >KAA0031258.1 allene oxid...	[more]
XP_022952977.1	9.1e-241	86.40	allene oxide synthase 3-like [Cucurbita moschata]	[more]
KAG6572010.1	1.2e-240	86.22	Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi11G010520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42650.1	3.4e-145	53.21	allene oxide synthase	[more]
AT4G15440.1	6.2e-78	38.36	hydroperoxide lyase 1	[more]
AT3G20120.1	5.7e-07	25.40	cytochrome P450, family 705, subfamily A, polypeptide 21	[more]
AT3G20120.2	5.7e-07	25.40	cytochrome P450, family 705, subfamily A, polypeptide 21	[more]
AT5G25180.1	1.7e-06	27.27	cytochrome P450, family 71, subfamily B, polypeptide 14	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 400..414 score: 16.92 coord: 416..434 score: 31.17 coord: 363..379 score: 37.1
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 339..446 e-value: 3.4E-17 score: 62.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 42..510 e-value: 7.4E-111 score: 373.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 42..493
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 43..489
None	No IPR available	PANTHER	PTHR24286:SF302	HYDROPEROXIDE LYASE	coord: 43..489

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G010520.1	Lsi11G010520.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen