Lsi11G010360 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G010360
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	chr11: 17852243 .. 17853129 (-)
RNA-Seq Expression	Lsi11G010360
Synteny	Lsi11G010360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTAGCCTAGTGGTAAAAGAGGAGACATAGTCTTAATAAATGACTAAGATGTCATAGGTTCAATCCATGATAACCACCTACCTAGAAATTGTTAGAGGTTTGATTCTCTCCTCTCACATTTGTCAGAAAAAAAAATGTGGCTAATTTTGTAAATTTAATATCTATGCCACTAACAACTATTTGAATTCAGTTCAAAATCAATCATTTTTATTCTTAAATAATACAAGCATGACTTGCAATTATCAAATTTAAAAAAAAAAATCAACTAACAAAGACTTATTGTACACAAAAGCACATACTAAAATAGGATCAAATAGAGACAAAATTTATAATTTAACCTATTTCAAGATTAACGTGAGATTGAGCAAGGAGGCTTGCGAAGAATATGTTGAGGAATTGGAGAAACTTGGCCACAAATTGATGGAATTGATCACCGTTAGCTTGGGATTACCAGCGGAAAGGTTTTGAGATTATTTCAAAGAGCAAACAAGTAATGTGAGACTGAATCAATATCCATCGTGCCCATCGCCGGAGTTAGCTCTCGGCGTCGGCCGCCACAAGGATTCTAGAGTCTTGACAGTGCTCTCCCAAGACGATGTTGGAGGGCTTGAAGTGAAAAGGAAGAAAGATGGAGAGTGGGTTCCAGTCAAACCTATACCAAATTCTTACATTGTGAATGTCGGAGATATTATTCAGGTACGTAGTAATACCATTGTATATATGATTCTTTATCCACTAAATTATGCTCAAATTAACTTTTCTATAAGCACTTTCCAAATAGAGTACAAATGATTTTTTTTTTTTTTTTAAGAACTTTTTAAAGCACTCTTGTACTAATAGGTTTGGAGCAATGAGAAGTATGAGAGTGTGGAGCACATGGTCA

mRNA sequence

ATGGGATCAAATAGAGACAAAATTTATAATTTAACCTATTTCAAGATTAACGTGAGATTGAGCAAGGAGGCTTGCGAAGAATATGTTGAGGAATTGGAGAAACTTGGCCACAAATTGATGGAATTGATCACCGTTAGCTTGGGATTACCAGCGGAAAGTAATGTGAGACTGAATCAATATCCATCGTGCCCATCGCCGGAGTTAGCTCTCGGCGTCGGCCGCCACAAGGATTCTAGAGTCTTGACAGTGCTCTCCCAAGACGATGTTGGAGGGCTTGAAGTGAAAAGGAAGAAAGATGGAGAGTGGGTTCCAGTCAAACCTATACCAAATTCTTACATTGTGAATGTCGGAGATATTATTCAGAACTTTTTAAAGCACTCTTGTACTAATAGGTTTGGAGCAATGAGAAGTATGAGAGTGTGGAGCACATGGTCA

Coding sequence (CDS)

ATGGGATCAAATAGAGACAAAATTTATAATTTAACCTATTTCAAGATTAACGTGAGATTGAGCAAGGAGGCTTGCGAAGAATATGTTGAGGAATTGGAGAAACTTGGCCACAAATTGATGGAATTGATCACCGTTAGCTTGGGATTACCAGCGGAAAGTAATGTGAGACTGAATCAATATCCATCGTGCCCATCGCCGGAGTTAGCTCTCGGCGTCGGCCGCCACAAGGATTCTAGAGTCTTGACAGTGCTCTCCCAAGACGATGTTGGAGGGCTTGAAGTGAAAAGGAAGAAAGATGGAGAGTGGGTTCCAGTCAAACCTATACCAAATTCTTACATTGTGAATGTCGGAGATATTATTCAGAACTTTTTAAAGCACTCTTGTACTAATAGGTTTGGAGCAATGAGAAGTATGAGAGTGTGGAGCACATGGTCA

Protein sequence

MGSNRDKIYNLTYFKINVRLSKEACEEYVEELEKLGHKLMELITVSLGLPAESNVRLNQYPSCPSPELALGVGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQNFLKHSCTNRFGAMRSMRVWSTWS

Homology

[Close]

BLAST of Lsi11G010360 vs. ExPASy Swiss-Prot
Match: P0C5H5 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.8e-19
Identity = 51/119 (42.86%), Postives = 70/119 (58.82%), Query Frame = 0


Query: 26  EEYVEELEKLGHKLMELITVSLGL----------PAESNVRLNQYPSCPSPELALGVGRH 85
           ++Y EE+++L   +MEL+ +SLG+           + S +R N YP CP PE  LG G H
Sbjct: 190 QKYCEEMKELSLTIMELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPH 249

Query: 86  KDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGD---IIQNFLKHSCTNR 132
            D   LT+L QDDVGGLEV    DGEW PV P+P + ++N+GD    + N    SC +R
Sbjct: 250 CDPTALTILLQDDVGGLEV--LVDGEWRPVSPVPGAMVINIGDTFMALSNGRYKSCLHR 306

[Close]

BLAST of Lsi11G010360 vs. ExPASy Swiss-Prot
Match: Q0JH50 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 1.8e-19
Identity = 51/119 (42.86%), Postives = 70/119 (58.82%), Query Frame = 0


Query: 26  EEYVEELEKLGHKLMELITVSLGL----------PAESNVRLNQYPSCPSPELALGVGRH 85
           ++Y EE+++L   +MEL+ +SLG+           + S +R N YP CP PE  LG G H
Sbjct: 190 QKYCEEMKELSLTIMELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPH 249

Query: 86  KDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGD---IIQNFLKHSCTNR 132
            D   LT+L QDDVGGLEV    DGEW PV P+P + ++N+GD    + N    SC +R
Sbjct: 250 CDPTALTILLQDDVGGLEV--LVDGEWRPVSPVPGAMVINIGDTFMALSNGRYKSCLHR 306

[Close]

BLAST of Lsi11G010360 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.5e-18
Identity = 46/110 (41.82%), Postives = 67/110 (60.91%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +E   EY   +  L   L+E I+ SLGL  +           ++ +N YP CP PEL  G
Sbjct: 156 REVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYG 215

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           +  HKD+ ++TVL QD+V GL+V   KDG+W+ V P+PN++IVN+GD +Q
Sbjct: 216 LPGHKDANLITVLLQDEVSGLQV--FKDGKWIAVNPVPNTFIVNLGDQMQ 263

[Close]

BLAST of Lsi11G010360 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.5e-18
Identity = 46/111 (41.44%), Postives = 65/111 (58.56%), Query Frame = 0


Query: 26  EEYVEELEKLGHKLMELITVSLGLPAE-----------------SNVRLNQYPSCPSPEL 85
           +EYV+E+EKL  +L+  I  SLG+  E                   +RLN YP CP PE 
Sbjct: 161 QEYVDEMEKLASRLLYCILGSLGVTVEDIEWAHKLEKSGSKVGRGAIRLNHYPVCPEPER 220

Query: 86  ALGVGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDI 120
           A+G+  H DS +LT+L Q + GGL+V R++ G WV V+P P   +VN+GD+
Sbjct: 221 AMGLAAHTDSTILTILHQSNTGGLQVFREESG-WVTVEPAPGVLVVNIGDL 270

[Close]

BLAST of Lsi11G010360 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-17
Identity = 48/110 (43.64%), Postives = 65/110 (59.09%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +E   EY   +  L  +L+E I+ SLGL ++           ++  N YP CP PEL  G
Sbjct: 158 REVTAEYATSVRALVLRLLEAISESLGLESDHISNILGKHAQHMAFNYYPPCPEPELTYG 217

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           +  HKD  V+TVL QD V GL+V   KD +WV V PIPN++IVN+GD +Q
Sbjct: 218 LPGHKDPTVITVLLQDQVSGLQV--FKDDKWVAVSPIPNTFIVNIGDQMQ 265

[Close]

BLAST of Lsi11G010360 vs. ExPASy TrEMBL
Match: A0A0A0K2S1 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074930 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 6.8e-38
Identity = 81/112 (72.32%), Postives = 92/112 (82.14%), Query Frame = 0


Query: 20  LSKEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELA 79
           +S+E CE YVEELEKLGHKLMELI  SLGLPA+          S+VRLN YP CPSPELA
Sbjct: 1   MSREICEGYVEELEKLGHKLMELIATSLGLPAKRFGEYFKGQTSSVRLNHYPLCPSPELA 60

Query: 80  LGVGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           LGVGRHKD  VLT+L+QDD+GGLEVKRKKDGEW+ VKP+P+SY V+VGDIIQ
Sbjct: 61  LGVGRHKDPGVLTLLAQDDIGGLEVKRKKDGEWIQVKPVPDSYAVDVGDIIQ 112

[Close]

BLAST of Lsi11G010360 vs. ExPASy TrEMBL
Match: A0A5A7SKK1 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G00230 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 3.2e-35
Identity = 76/110 (69.09%), Postives = 88/110 (80.00%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +E CE YVEELEKLGHKLMELI  SLGLP +          S+VRLN YP CPSPELALG
Sbjct: 160 REICEGYVEELEKLGHKLMELIANSLGLPGKRFRDYFKGQTSSVRLNHYPLCPSPELALG 219

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD  VLTVL+QDD+GGLEV+RK+DG W+ VKP+P+S++VNVGDI Q
Sbjct: 220 VGRHKDPGVLTVLAQDDIGGLEVRRKRDGAWIQVKPVPDSFVVNVGDITQ 269

[Close]

BLAST of Lsi11G010360 vs. ExPASy TrEMBL
Match: A0A6J1GLY0 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111455576 PE=3 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 3.2e-35
Identity = 80/111 (72.07%), Postives = 90/111 (81.08%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           ++ACEEYVEELEKLGHKLMEL+ VSLGLPAE          S  RLN YP CPSPELALG
Sbjct: 155 RKACEEYVEELEKLGHKLMELLAVSLGLPAERFRDYFKETTSFGRLNHYPPCPSPELALG 214

Query: 82  VGRHKDSRVLTVLSQ-DDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD  VLTVL+Q DDV GLEVKRK+DGEW+ VKP+P+SY+VNVG+I Q
Sbjct: 215 VGRHKDPGVLTVLAQDDDVEGLEVKRKRDGEWIRVKPVPDSYVVNVGEITQ 265

[Close]

BLAST of Lsi11G010360 vs. ExPASy TrEMBL
Match: A0A6J1I8C6 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111470930 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.1e-35
Identity = 80/111 (72.07%), Postives = 89/111 (80.18%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           + ACEEYVEELEKLGHKLMEL+ VSLGLPAE          S  RLN YP CPSPELALG
Sbjct: 155 RRACEEYVEELEKLGHKLMELLAVSLGLPAERFRDYFKETTSFGRLNHYPPCPSPELALG 214

Query: 82  VGRHKDSRVLTVLSQ-DDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD  VLTVL+Q DDV GLEVKRK+DGEW+ VKP+P+SY+VNVG+I Q
Sbjct: 215 VGRHKDPGVLTVLAQDDDVEGLEVKRKRDGEWIRVKPVPDSYVVNVGEITQ 265

[Close]

BLAST of Lsi11G010360 vs. ExPASy TrEMBL
Match: A0A5D3C5Z7 (Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00230 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.1e-35
Identity = 76/109 (69.72%), Postives = 87/109 (79.82%), Query Frame = 0


Query: 23  EACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALGV 82
           E CE YVEELEKLGHKLMELI  SLGLP +          S+VRLN YP CPSPELALGV
Sbjct: 160 EICEGYVEELEKLGHKLMELIANSLGLPGKRFRDYFKGQTSSVRLNHYPLCPSPELALGV 219

Query: 83  GRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           GRHKD  VLTVL+QDD+GGLEV+RK+DG W+ VKP+P+S++VNVGDI Q
Sbjct: 220 GRHKDPGVLTVLAQDDIGGLEVRRKRDGAWIQVKPVPDSFVVNVGDITQ 268

[Close]

BLAST of Lsi11G010360 vs. NCBI nr
Match: XP_038887395.1 (protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida])

HSP 1 Score: 173.3 bits (438), Expect = 1.5e-39
Identity = 86/110 (78.18%), Postives = 93/110 (84.55%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +EACEEYVEELEKLGHKLMELI VSLGLPA+          S VRLN YPSCPSPELALG
Sbjct: 158 REACEEYVEELEKLGHKLMELIAVSLGLPAKRFRDYFKEQTSFVRLNHYPSCPSPELALG 217

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD  VLTVL+QDDVGGLEVKRK+DGEWV VKP+P+SY+VNVGDI Q
Sbjct: 218 VGRHKDPSVLTVLAQDDVGGLEVKRKRDGEWVRVKPVPDSYVVNVGDITQ 267

[Close]

BLAST of Lsi11G010360 vs. NCBI nr
Match: KAF5444018.1 (hypothetical protein F2P56_036525 [Juglans regia])

HSP 1 Score: 163.7 bits (413), Expect = 1.2e-36
Identity = 82/135 (60.74%), Postives = 98/135 (72.59%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +EACE+Y  E+EKL +KL+ELI++SLGL A           + +RLN YP CP P LALG
Sbjct: 156 REACEDYAREVEKLAYKLLELISMSLGLSANRLNGYFKDQTTFLRLNHYPPCPFPHLALG 215

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQNFLK---HSC 141
           VG HKD+  LTVL+QDDVGGLEV+RK D EW+PVKP  ++YI+NVGDIIQ   K     C
Sbjct: 216 VGHHKDAGGLTVLAQDDVGGLEVRRKSDREWIPVKPTTDAYIINVGDIIQGIRKIINRVC 275

Query: 142 TNRFGAMRSMRVWST 144
             RFGAMRSMRVWST
Sbjct: 276 LYRFGAMRSMRVWST 290

[Close]

BLAST of Lsi11G010360 vs. NCBI nr
Match: XP_031744488.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X1 [Cucumis sativus] >KAE8645874.1 hypothetical protein Csa_017140 [Cucumis sativus])

HSP 1 Score: 163.7 bits (413), Expect = 1.2e-36
Identity = 80/111 (72.07%), Postives = 90/111 (81.08%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE-----------SNVRLNQYPSCPSPELAL 81
           +E CE YVEELEKLGHKLMELI  SLGLPA+           S+VRLN YP CPSPELAL
Sbjct: 160 REICEGYVEELEKLGHKLMELIATSLGLPAKRFGEYFKAGQTSSVRLNHYPLCPSPELAL 219

Query: 82  GVGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           GVGRHKD  VLT+L+QDD+GGLEVKRKKDGEW+ VKP+P+SY V+VGDIIQ
Sbjct: 220 GVGRHKDPGVLTLLAQDDIGGLEVKRKKDGEWIQVKPVPDSYAVDVGDIIQ 270

[Close]

BLAST of Lsi11G010360 vs. NCBI nr
Match: KAA0031270.1 (protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 157.9 bits (398), Expect = 6.5e-35
Identity = 76/110 (69.09%), Postives = 88/110 (80.00%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +E CE YVEELEKLGHKLMELI  SLGLP +          S+VRLN YP CPSPELALG
Sbjct: 160 REICEGYVEELEKLGHKLMELIANSLGLPGKRFRDYFKGQTSSVRLNHYPLCPSPELALG 219

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD  VLTVL+QDD+GGLEV+RK+DG W+ VKP+P+S++VNVGDI Q
Sbjct: 220 VGRHKDPGVLTVLAQDDIGGLEVRRKRDGAWIQVKPVPDSFVVNVGDITQ 269

[Close]

BLAST of Lsi11G010360 vs. NCBI nr
Match: KAG6572000.1 (putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 157.9 bits (398), Expect = 6.5e-35
Identity = 80/111 (72.07%), Postives = 90/111 (81.08%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           ++ACEEYVEELEKLGHKLMEL+ VSLGLPAE          S  RLN YP CPSPELALG
Sbjct: 155 RKACEEYVEELEKLGHKLMELLAVSLGLPAERFRDYFKETTSFGRLNHYPPCPSPELALG 214

Query: 82  VGRHKDSRVLTVLSQ-DDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD  VLTVL+Q DDV GLEVKRK+DGEW+ VKP+P+SY+VNVG+I Q
Sbjct: 215 VGRHKDPGVLTVLAQDDDVEGLEVKRKRDGEWIRVKPVPDSYVVNVGEITQ 265

[Close]

BLAST of Lsi11G010360 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-31
Identity = 60/110 (54.55%), Postives = 81/110 (73.64%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +E C+EY  E+EKL  +L+EL+++SLGLP +          S +R N YP CP+PELALG
Sbjct: 159 REVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALG 218

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD   LTVL+QD VGGL+V R+ DG+W+PVKPI ++ I+N+G+ IQ
Sbjct: 219 VGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQ 268

[Close]

BLAST of Lsi11G010360 vs. TAIR 10
Match: AT3G19000.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.2e-31
Identity = 60/110 (54.55%), Postives = 81/110 (73.64%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +E C+EY  E+EKL  +L+EL+++SLGLP +          S +R N YP CP+PELALG
Sbjct: 159 REVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYPPCPNPELALG 218

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VGRHKD   LTVL+QD VGGL+V R+ DG+W+PVKPI ++ I+N+G+ IQ
Sbjct: 219 VGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQ 268

[Close]

BLAST of Lsi11G010360 vs. TAIR 10
Match: AT3G19010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 6.7e-30
Identity = 56/110 (50.91%), Postives = 80/110 (72.73%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +EACE Y    EKL  KL+ELI++SLGLP E          S  R+N+YP CP P+LALG
Sbjct: 154 REACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALG 213

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VG HKD+ V+++L+QDDVGGL+V R+ DG W P++P+PN+ ++N+G+ ++
Sbjct: 214 VGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNCME 263

[Close]

BLAST of Lsi11G010360 vs. TAIR 10
Match: AT3G19010.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 127.9 bits (320), Expect = 6.7e-30
Identity = 56/110 (50.91%), Postives = 80/110 (72.73%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAE----------SNVRLNQYPSCPSPELALG 81
           +EACE Y    EKL  KL+ELI++SLGLP E          S  R+N+YP CP P+LALG
Sbjct: 154 REACEVYARHAEKLAFKLLELISLSLGLPKERFHDYFKEQMSFFRINRYPPCPRPDLALG 213

Query: 82  VGRHKDSRVLTVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           VG HKD+ V+++L+QDDVGGL+V R+ DG W P++P+PN+ ++N+G+ ++
Sbjct: 214 VGHHKDADVISLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNCME 263

[Close]

BLAST of Lsi11G010360 vs. TAIR 10
Match: AT3G19010.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 6.8e-22
Identity = 43/100 (43.00%), Postives = 64/100 (64.00%), Query Frame = 0


Query: 22  KEACEEYVEELEKLGHKLMELITVSLGLPAESNVRLNQYPSCPSPELALGVGRHKDSRVL 81
           +EACE Y    EKL                     +N+YP CP P+LALGVG HKD+ V+
Sbjct: 154 REACEVYARHAEKLA--------------------INRYPPCPRPDLALGVGHHKDADVI 213

Query: 82  TVLSQDDVGGLEVKRKKDGEWVPVKPIPNSYIVNVGDIIQ 122
           ++L+QDDVGGL+V R+ DG W P++P+PN+ ++N+G+ ++
Sbjct: 214 SLLAQDDVGGLQVSRRSDGVWFPIRPVPNALVINIGNCME 233

The following BLAST results are available for this feature:

BLAST of Lsi11G010360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C5H5	1.8e-19	42.86	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV...	[more]
Q0JH50	1.8e-19	42.86	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA20OX2 PE=...	[more]
Q9ZSA7	7.5e-18	41.82	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q9C971	7.5e-18	41.44	Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ...	[more]
Q9ZSA8	1.3e-17	43.64	Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1	[more]

BLAST of Lsi11G010360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2S1	6.8e-38	72.32	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G...	[more]
A0A5A7SKK1	3.2e-35	69.09	Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C...	[more]
A0A6J1GLY0	3.2e-35	72.07	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX...	[more]
A0A6J1I8C6	4.1e-35	72.07	probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X1 OS=Cucurbita ...	[more]
A0A5D3C5Z7	4.1e-35	69.72	Protein DMR6-LIKE OXYGENASE 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]

BLAST of Lsi11G010360 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038887395.1	1.5e-39	78.18	protein DMR6-LIKE OXYGENASE 2-like [Benincasa hispida]	[more]
KAF5444018.1	1.2e-36	60.74	hypothetical protein F2P56_036525 [Juglans regia]	[more]
XP_031744488.1	1.2e-36	72.07	probable 2-oxoglutarate-dependent dioxygenase At5g05600 isoform X1 [Cucumis sati...	[more]
KAA0031270.1	6.5e-35	69.09	protein DMR6-LIKE OXYGENASE 2-like [Cucumis melo var. makuwa]	[more]
KAG6572000.1	6.5e-35	72.07	putative 2-oxoglutarate-dependent dioxygenase, partial [Cucurbita argyrosperma s...	[more]

BLAST of Lsi11G010360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19000.1	1.2e-31	54.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.2	1.2e-31	54.55	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.1	6.7e-30	50.91	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.2	6.7e-30	50.91	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19010.3	6.8e-22	43.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 53..121 e-value: 2.6E-17 score: 63.1
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 6..142 e-value: 9.1E-34 score: 119.4
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 18..122
None	No IPR available	PANTHER	PTHR10209:SF727	DIRIGENT PROTEIN	coord: 18..122
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 22..121
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 50..145 score: 9.767441

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G010360.1	Lsi11G010360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding