Lsi11G007440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G007440
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description(R)-mandelonitrile lyase 1-like
Locationchr11: 8174773 .. 8181268 (-)
RNA-Seq ExpressionLsi11G007440
SyntenyLsi11G007440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACAATTAGTATGGAGCATTCTATGGCTACCATTCTCTTATTCATCCTCATCTCCATCTTTCAATTAGGAGTTCTCTCCTCCCACAATATACCCAATCAAGGTAACAACCCTCTCTAATTCTTTTTTTTTTTTTAATATATATATGTAATGTGGGTTTGTAAGTGCACTTGAATTCTTTTTTTTTTTAAGCTATTATTGTTATAATAACATTGATATAATACTAAATTTATTATAACTCACAAATTTAAGTTTTGGATTTATTAGTAATTTAACATGTATCAGATTAGGAGTCTAGAATACATTTCCTCCCTAGCTAATATTAAATTGACAATTAAGTAAAAAATCACTTTTAGTCTCTAAACTTTCATAAAAGTAACAATTTAGTCTTTGAATTTTGGTTTGTAATGATTTAGTCCCTATACTTTGAATTTTGTAACAATTTAGTTTGTGAACTTTATTATGTAACAATCTAGTCTTTGTACTTTAAAATAACAATTTAGTTCCTATTGTAGAAATTAGTGTTAAGATTTAATGTGTCGATAAACTAATTAGAGACTCAACATTTTGATATAATACAGAGTTTACATCATAAAATAATAAAAATTAACATCTAATTTTGATTTTTTTTCTTTTACGTTCGAGATTAAATTATTACAAATTTAAAAGTACAAAGACCAAATTGTTACATACTAAAGTTTCGAGATTAAATTGTTACAAAATTAAAAGTATAAGGACTAAATCGTTACAAACATAAGTTCACAGAGTCTAAGTTGTTACTTTCGTGAAAGTTTAAAGACTAAAAGTGTTTTTTCACCTTGAATTGATGGGTGTTTTTGGAGCATAAAAGTGAGGGAGATTTAACAATTGTTATTGTGTTTGGAACTTTTCTTTTTCAGATACTAGTTACATGAAGTTTGTACATGAGGCCAGTGAGTTACCAGAAAAAGAAGAATATGACTACATAATAATAGGAGGAGGAACAGCAGGCTGTCCATTAGCTGCGACATTATCATCAAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCTAACAAATATCCCTCTGTATTAAATGAACAAGGTCTATTGAATGTGTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACAGTTTATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGTGTTCTTGGTGGTGGTAGCATGGCCAATGCCGGCTTCTACTCGAGGGGTCATCGAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGGACATGGAAATGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAAGCCAAGTTTGAGTGCTTGGCAATCTGCTTTTAGGAGTGCTTTGTTGGAAGGTGGGGTTGGTGCTGATAATGGGTTTGATTTGAGGCACCATGTGGGGACTAAAACTGGAGGGTCTATCTTTGATAACAAAGGAAACAGACATGGAGCTGTGGAACTTCTCAACAAGGCTGAACCCCAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAAAATCATCTTCTCTGGTTAGTTCCTATCAAATCTTTGGTTTAAATTTTATGTCGGTTAATGAATTTTTGAAAATATACCATTTGTATATGTTTTCTTGTTACTAAATATATTATCCACTCACACTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATACACATACATATATATATATACATACATACATACGAGAGAAAAAATTATTTGAAGAGACTGTCACTATAGAAAAATTATGAAGCCACCACAATGTACTACGCAACTTTACAAATAGAAAAATTATGAAGCCACCACAATGTACTAGGCAATTTTACACTATTGGTCGATGGGTAAGAGAAAAATAGGAAAAGAAAGGATAATTGCGCAAAACTTTTGGAAATATTTTCAAATATAACAAAATGTCACTGTCTATCGGTGATAAATATCGATAGATGTGACATCAGTGATAGATAATAGTACTCTATCAATGTACTCTATCAATGTCTATCAGTGTCTATCACTGATAGACAATGACATTTTGCTATATTTATAAATAAATTAGCTCATTTTGCTATATTTGAAAATAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

AAAACAATTAGTATGGAGCATTCTATGGCTACCATTCTCTTATTCATCCTCATCTCCATCTTTCAATTAGGAGTTCTCTCCTCCCACAATATACCCAATCAAGATACTAGTTACATGAAGTTTGTACATGAGGCCAGTGAGTTACCAGAAAAAGAAGAATATGACTACATAATAATAGGAGGAGGAACAGCAGGCTGTCCATTAGCTGCGACATTATCATCAAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCTAACAAATATCCCTCTGTATTAAATGAACAAGGTCTATTGAATGTGTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACAGTTTATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGTGTTCTTGGTGGTGGTAGCATGGCCAATGCCGGCTTCTACTCGAGGGGTCATCGAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGGACATGGAAATGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAAGCCAAGTTTGAGTGCTTGGCAATCTGCTTTTAGGAGTGCTTTGTTGGAAGGTGGGGTTGGTGCTGATAATGGGTTTGATTTGAGGCACCATGTGGGGACTAAAACTGGAGGGTCTATCTTTGATAACAAAGGAAACAGACATGGAGCTGTGGAACTTCTCAACAAGGCTGAACCCCAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAAAATCATCTTCTCTGGTTTATCAGCAAATGGGGTTTCATATTCAGATTCGAAAGGAAAGTTGCATACAGCATTCATTCGCAAGAAAGGAGAGATTATTTTGAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTCCTCGACCAACCACATGTCGGAGAATTCATGTCGGACAATCCCCGTTTCAGTCCCACCATTGTACTTCCATTCCAACTAGTTGCTTCATCTGCACAAGTCGTTGGAACATTAGACAACAATATCTACTTGCAATCATTTGCTAGCCCTTTACCATTTTTTGCTCCACCATCTTTTAGTCTTCTTCCTCCTCAATCCACTTCTATCGTCCCTAGCGTAGCCATTTTTGTTGGAAAATTCTCTGATGTCCATTCTGAGGGGTCACTCCGATTGAATTCTTCCACTGATGTGAAGAATAATCCTATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTAAAACTCAAACCATGGAAAAGTTTAAGACCCAAGATTTGGAGGGTAATAAAGGATTTCTGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTGGGATGATAGCTCCGTTGAAGAATACTGTCAGAAGACGGTGATGACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAACCACAAAGTCATCGGAATTGAGAATTTGCGTGTAGTGGACGGATCCACTTTCTCTGTCTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTATGTTGGCCTCACGGTGCTGCAACAAAGATCAAGTTAAAAGAGTGCAATAACTAAGAGATACCATTTTTTTTTTTTTGAGAAAAGCATGTATCTTGTTTTTAATTATTATTATTGGAAATAAAAATATTTTTTTCTCAAGTTCAACAA

Coding sequence (CDS)

ATGGAGCATTCTATGGCTACCATTCTCTTATTCATCCTCATCTCCATCTTTCAATTAGGAGTTCTCTCCTCCCACAATATACCCAATCAAGATACTAGTTACATGAAGTTTGTACATGAGGCCAGTGAGTTACCAGAAAAAGAAGAATATGACTACATAATAATAGGAGGAGGAACAGCAGGCTGTCCATTAGCTGCGACATTATCATCAAAATTTTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCTAACAAATATCCCTCTGTATTAAATGAACAAGGTCTATTGAATGTGTTTGTTGCAGAAGATGATGGCCAAAATCCCTTCCAACAGTTTATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGTGTTCTTGGTGGTGGTAGCATGGCCAATGCCGGCTTCTACTCGAGGGGTCATCGAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGGACATGGAAATGGTGGAGAAGGCTTATGAATGGGTTGAAGAAACTGTGGTATCTAAGCCAAGTTTGAGTGCTTGGCAATCTGCTTTTAGGAGTGCTTTGTTGGAAGGTGGGGTTGGTGCTGATAATGGGTTTGATTTGAGGCACCATGTGGGGACTAAAACTGGAGGGTCTATCTTTGATAACAAAGGAAACAGACATGGAGCTGTGGAACTTCTCAACAAGGCTGAACCCCAAAACCTTAAAGTTGCAACTCAGGCCACAGTCCAAAAAATCATCTTCTCTGGTTTATCAGCAAATGGGGTTTCATATTCAGATTCGAAAGGAAAGTTGCATACAGCATTCATTCGCAAGAAAGGAGAGATTATTTTGAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTCCTCGACCAACCACATGTCGGAGAATTCATGTCGGACAATCCCCGTTTCAGTCCCACCATTGTACTTCCATTCCAACTAGTTGCTTCATCTGCACAAGTCGTTGGAACATTAGACAACAATATCTACTTGCAATCATTTGCTAGCCCTTTACCATTTTTTGCTCCACCATCTTTTAGTCTTCTTCCTCCTCAATCCACTTCTATCGTCCCTAGCGTAGCCATTTTTGTTGGAAAATTCTCTGATGTCCATTCTGAGGGGTCACTCCGATTGAATTCTTCCACTGATGTGAAGAATAATCCTATTGTTCGATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGTGTTAGAGGAGTAAGAAAAGTGGGAGATTTGCTTAAAACTCAAACCATGGAAAAGTTTAAGACCCAAGATTTGGAGGGTAATAAAGGATTTCTGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTGGGATGATAGCTCCGTTGAAGAATACTGTCAGAAGACGGTGATGACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTGGTCGATGATAACCACAAAGTCATCGGAATTGAGAATTTGCGTGTAGTGGACGGATCCACTTTCTCTGTCTCACCGGGAACTAATCCTATGGCCACCCTCATGATGCTCGGCCGGTATGTTGGCCTCACGGTGCTGCAACAAAGATCAAGTTAA

Protein sequence

MEHSMATILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAPPSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRSS
Homology
BLAST of Lsi11G007440 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 4.5e-156
Identity = 278/545 (51.01%), Postives = 381/545 (69.91%), Query Frame = 0

Query: 4   SMATILLFILISIFQLGVLSSHNI---PNQDTSYMKFVHEASELPEKEEYDYIIIGGGTA 63
           +M+ ILL + + +  L     H++    N D SY++F ++A++L  +  YDY+I+GGGT+
Sbjct: 5   TMSAILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTS 64

Query: 64  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVEN 123
           GCPLAATLS K+ VL+LERGS P  YP+VL   G +     EDDG+ P ++F+SEDG++N
Sbjct: 65  GCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDN 124

Query: 124 IRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSA 183
           +RGRVLGG SM NAG Y+R +   + ++GVDWDM++V K YEWVE+T+V KP+   WQS 
Sbjct: 125 VRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSV 184

Query: 184 FRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATV 243
             +A LE GV  ++GF L H  GT+  GS FDNKG RH A ELLNK    NL+V   A+V
Sbjct: 185 TGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 244

Query: 244 QKIIFS---GLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHL 303
           +KIIFS   GL+A GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSGVGP+S+L
Sbjct: 245 EKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 304

Query: 304 SSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPF 363
           SSL +PVVL  P+VG+F+ DNPR    I+ P  +  +   V+G + N+ Y  SF+S LPF
Sbjct: 305 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS-LPF 364

Query: 364 FAPPSFSLLPPQSTSIVPSV-AIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPD 423
             PP FS  P  S  +  S  A F  K +   S GSL L SS++V+ +P V+FNYYS+P 
Sbjct: 365 TTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPT 424

Query: 424 DLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTV 483
           DL+ CV G++K+G+LL T  ++ +K +DL G +GF  LG+PLP++  DD++ E +C+++V
Sbjct: 425 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESV 484

Query: 484 MTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVL 542
            +YWHYHGGCLVGKV+D + +V GI+ LRVVDGSTF  +P ++P    +MLGRYVG+ +L
Sbjct: 485 ASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKIL 544

BLAST of Lsi11G007440 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 2.1e-153
Identity = 274/545 (50.28%), Postives = 379/545 (69.54%), Query Frame = 0

Query: 4   SMATILLFILISIFQLGVLSSHNI---PNQDTSYMKFVHEASELPEKEEYDYIIIGGGTA 63
           +M+ ILL + I +  L     H++    + D SY+ F ++A++L  +  YDY+I+GGGT+
Sbjct: 5   TMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTS 64

Query: 64  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVEN 123
           GCPLAATLS K+ VL+LERGS P  YP+VL   G +     EDDG+ P ++F+SEDG++N
Sbjct: 65  GCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDN 124

Query: 124 IRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSA 183
           +RGRVLGG S+ NAG Y+R +   + ++GVDWDM++V + YEWVE+T+V KP+  +WQS 
Sbjct: 125 VRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSV 184

Query: 184 FRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATV 243
            ++A LE GV  ++GF L H  GT+  GS FDNKG RH A ELLNK    NL+V   A+V
Sbjct: 185 TKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASV 244

Query: 244 QKIIFS---GLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHL 303
           +KIIFS   GL+A GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSGVGP+S+L
Sbjct: 245 EKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYL 304

Query: 304 SSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPF 363
           SSL +PVVL  P+VG+F+ DNPR    I+ P  +  +   V+G + N+ Y  SF+S LPF
Sbjct: 305 SSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLG-ISNDFYQCSFSS-LPF 364

Query: 364 FAPPSFSLLPPQSTSIVPSV-AIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPD 423
             PP F   P  S  +  S  A F  K +   S GSL L SS++V+ +P V+FNYYS+  
Sbjct: 365 TTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLT 424

Query: 424 DLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTV 483
           DL+ CV G++K+G+LL T  ++ +K +DL G +GF  LG+PLP++  DD++ E +C+++V
Sbjct: 425 DLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESV 484

Query: 484 MTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVL 542
            +YWHYHGGCLVGKV+D + +V GI  LRVVDGSTF  +P ++P    +MLGRYVG+ +L
Sbjct: 485 ASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKIL 544

BLAST of Lsi11G007440 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.9e-151
Identity = 272/549 (49.54%), Postives = 376/549 (68.49%), Query Frame = 0

Query: 1   MEHSMATILLFILISIFQL---GVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGG 60
           ++ +M+ +LL + I +  L    V S  N  + D SY+ FV++A++   +  YDYII+GG
Sbjct: 2   VKSTMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGG 61

Query: 61  GTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDG 120
           GTAGCPLAATLS+ +SVL+LERGS P +YP++L   G +     EDDG+ P ++F+SEDG
Sbjct: 62  GTAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDG 121

Query: 121 VENIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAW 180
           ++N+RGRVLGG SM NAG Y R +  FF   G++WDM++V + YEWVE+T+V +P    W
Sbjct: 122 IDNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTW 181

Query: 181 QSAFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQ 240
           Q+   +A LE G+  +NGF + H  GT+  GS FDN G RH + ELLNK +P NL+VA Q
Sbjct: 182 QTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQ 241

Query: 241 ATVQKIIF----SGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGP 300
           A V+KIIF    SG++A GV Y+DS G  H AF+R +GE+ILSAG IGSPQLLLLSGVGP
Sbjct: 242 AAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGP 301

Query: 301 KSHLSSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFAS 360
           +S+L+SL + VV   P+VG+++ DNPR    I+ P  + AS+  V+G   +  + Q   S
Sbjct: 302 ESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSIS 361

Query: 361 PLPFFAPPSFSLLPPQSTSIV-PSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYY 420
            LPF  PP FS  P  S  +   + A  V K     S G++ LNSS+DV+  P V+FNYY
Sbjct: 362 SLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYY 421

Query: 421 SHPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYC 480
           S+  DL+ CV G++K+G++L T  +E +K +DL G  GF  LG+PLPEN  DD++ E +C
Sbjct: 422 SNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFC 481

Query: 481 QKTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVG 540
           +++V +YWHYHGGCLVGKV+DD  +V GI  LRVVDGSTF  +P ++P    +MLGRY+G
Sbjct: 482 RESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMG 541

Query: 541 LTVLQQRSS 542
           + +LQ+RS+
Sbjct: 542 IQILQERSA 547

BLAST of Lsi11G007440 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 9.0e-149
Identity = 272/549 (49.54%), Postives = 367/549 (66.85%), Query Frame = 0

Query: 1   MEHSMATILLFIL-ISIFQLGVLSSHNIPN---QDTSYMKFVHEASELPEKEEYDYIIIG 60
           ME S  +++LF+L + +  L     H++ N    D SY+KFV+ A++   +  YDYI+IG
Sbjct: 1   MEKSTMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIG 60

Query: 61  GGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISED 120
           GGT+GCPLAATLS K+ VLLLERG+   +YP+ L   G       +DDG+ P ++F+SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSED 120

Query: 121 GVENIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSA 180
           G++N+R R+LGG ++ NAG Y+R +  F+   G++WD+++V K YEWVE+ +V KP+  +
Sbjct: 121 GIDNVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQS 180

Query: 181 WQSAFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVAT 240
           WQS      LE G+  DNGF L H  GT+  GS FDN G RH A ELLNK +P NL VA 
Sbjct: 181 WQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAV 240

Query: 241 QATVQKIIF----SGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVG 300
           QA+V+KI+F    S LSA GV Y+DS G  H AF+R  GE+I+SAG IG+PQLLLLSGVG
Sbjct: 241 QASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVG 300

Query: 301 PKSHLSSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFA 360
           P+S+LSSL + VV   P+VG+F+ +NPR       P  + AS   V+G   +  Y Q   
Sbjct: 301 PESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSL 360

Query: 361 SPLPFFAPPSFSLLPPQSTSIVPSV-AIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNY 420
           S LPF  PP FSL P  S  +  S  A  V +     S GS+ LNSS+DV+  P ++FNY
Sbjct: 361 SSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNY 420

Query: 421 YSHPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEY 480
           YS+  DLA CV G++K+GDLL+T+ +E +K +D+ G  GF +LG+PLPEN  DD+S E +
Sbjct: 421 YSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETF 480

Query: 481 CQKTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYV 540
           C   V +YWHYHGG LVGKV+DD+ +V+GI+ LRVVD STF   P ++P    +MLGRYV
Sbjct: 481 CLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYV 540

BLAST of Lsi11G007440 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.5e-148
Identity = 270/546 (49.45%), Postives = 369/546 (67.58%), Query Frame = 0

Query: 6   ATILLFILISIFQLGVLSSHNI---PNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGC 65
           +++L   L+ +  LGV+   N     N+   +M+F+  A++   ++ YDYII+GGGTAGC
Sbjct: 7   SSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGC 66

Query: 66  PLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIR 125
           PLAATLS  F VLLLERG  P   P+V++  G L      ++  +P Q FISE+GV N R
Sbjct: 67  PLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNAR 126

Query: 126 GRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFR 185
           GRVLGG S  NAGFYSR  ++FFE++G+ WD+  V ++YEWVE  +V +P L  WQ+A R
Sbjct: 127 GRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIR 186

Query: 186 SALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQK 245
            ALLE GV   NGF L H VGTK GGS FD  G RH + +LL  A   N++VA  ATV++
Sbjct: 187 DALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVER 246

Query: 246 IIF--------SGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPK 305
           ++         S +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL LSG+GP+
Sbjct: 247 VLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPR 306

Query: 306 SHLSSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASP 365
           S+LS+  +PV LDQPHVG+F+ DNPR   +IV P  +  S  QVVG  ++  +L++ ++ 
Sbjct: 307 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 366

Query: 366 LPFFAP-PSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYS 425
           +PF +P  S  +  P S   VP V   + K     S G LRL +STDV+ NP+VRFNY+S
Sbjct: 367 IPFASPLHSVFIRAPASPLYVP-VTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFS 426

Query: 426 HPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQ 485
            P DL RCV G RK+G++L+++ M+ F  ++  GN+ F F+G PLP +  +D  + ++C+
Sbjct: 427 DPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCR 486

Query: 486 KTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL 540
           +TV T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF++SPGTNP ATLMMLGRY+GL
Sbjct: 487 RTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGL 546

BLAST of Lsi11G007440 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.9e-282
Identity = 489/534 (91.57%), Postives = 510/534 (95.51%), Query Frame = 0

Query: 8   ILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPLAAT 67
           ILLF LISIFQLGV+SSH IPNQDTSYMKFVHEASEL E EEYDYIIIGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 68  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIRGRVLG 127
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLN FVAEDDGQNPFQ FISEDGVEN+RGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 128 GGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSALLE 187
           GGSM NAGFYSRGHREFFE+AGVDWDME+VEKAYEWVEETVVSKPSLS WQ+AFRSALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 188 GGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKIIFSG 247
           GGVG DNGFDL+H VGTKTGGSIFDNKGNRHGAVELLNK EP+N KVATQATVQ+IIF+G
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 248 LSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLD 307
           LSA+GVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SLKLPVVLD
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 308 QPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAPPSFSLLP 367
           QPHVGEFMSDNPRF+PTIVLPFQ+VASSAQVVGTLDNNI+LQ+FASPLPFFAPPSFSLLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 368 PQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVRK 427
           PQSTSIVPS+AIFVGKFSDV+SEGSLRLNSS DVK +PIVRFNYYSHPDDLARCVRGVRK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 428 VGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYWHYHGGCL 487
           VGDLL+T TMEK KTQDLEGNK F FLGL LPENL +DS+VEEYCQKTV TYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 488 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRSS 542
           VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL VLQQRSS
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

BLAST of Lsi11G007440 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.9e-282
Identity = 489/534 (91.57%), Postives = 510/534 (95.51%), Query Frame = 0

Query: 8   ILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPLAAT 67
           ILLF LISIFQLGV+SSH IPNQDTSYMKFVHEASEL E EEYDYIIIGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 68  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIRGRVLG 127
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLN FVAEDDGQNPFQ FISEDGVEN+RGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 128 GGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSALLE 187
           GGSM NAGFYSRGHREFFE+AGVDWDME+VEKAYEWVEETVVSKPSLS WQ+AFRSALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 188 GGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKIIFSG 247
           GGVG DNGFDL+H VGTKTGGSIFDNKGNRHGAVELLNK EP+N KVATQATVQ+IIF+G
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 248 LSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLD 307
           LSA+GVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SLKLPVVLD
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 308 QPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAPPSFSLLP 367
           QPHVGEFMSDNPRF+PTIVLPFQ+VASSAQVVGTLDNNI+LQ+FASPLPFFAPPSFSLLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 368 PQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVRK 427
           PQSTSIVPS+AIFVGKFSDV+SEGSLRLNSS DVK +PIVRFNYYSHPDDLARCVRGVRK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 428 VGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYWHYHGGCL 487
           VGDLL+T TMEK KTQDLEGNK F FLGL LPENL +DS+VEEYCQKTV TYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 488 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRSS 542
           VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL VLQQRSS
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

BLAST of Lsi11G007440 vs. ExPASy TrEMBL
Match: A0A0A0LK79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.6e-281
Identity = 488/542 (90.04%), Postives = 516/542 (95.20%), Query Frame = 0

Query: 1   MEHSMAT-ILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGT 60
           M++SMA  ILLF LISIFQLG+++SH IPNQDTSYMKFV+EASEL E EEYDYIIIGGGT
Sbjct: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVE 120
           AGCPLAATLSSKFSVLLLERG+DPNKYPSVLNEQGLLN FVAEDDGQNPFQ FISEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQS 180
           N+RGRVLGGGSM NAGFYSRGHR+FFE+AGVDWD E+VEKAYEWVEETVVSKPSLS WQ+
Sbjct: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180

Query: 181 AFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQAT 240
           AFRSALLEGGVG D GFDLRH VGTKTGGSIFDNKGNRHGAVELLNK EP+NLKVATQAT
Sbjct: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240

Query: 241 VQKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS 300
           VQ+IIF+GLSA+GVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300

Query: 301 LKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFA 360
           L+LPVVLDQPHVGEFMSDNPRFSPTIVLPFQLV+SSAQVVGTLD+NI+LQSFASPLPFFA
Sbjct: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ TSIVPS+AIFVGKFSDVHSEGSLRLNSS DVK +PIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTY 480
           RCVRGVRKVGDLLKT TMEK KTQDLEGNK F FLGL LPENL +D++VEEYCQKTV TY
Sbjct: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQR 540
           WHYHGGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGL +LQQR
Sbjct: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540

Query: 541 SS 542
           SS
Sbjct: 541 SS 542

BLAST of Lsi11G007440 vs. ExPASy TrEMBL
Match: A0A6J1FC90 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.8e-262
Identity = 459/541 (84.84%), Postives = 492/541 (90.94%), Query Frame = 0

Query: 1   MEHSMATILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTA 60
           +EHSMATIL  ILISIF L VLSS+ IPNQD SYMK VH+A+ELPE E+YDYIIIGGGTA
Sbjct: 4   IEHSMATILPLILISIFLLRVLSSNTIPNQDVSYMKLVHDATELPENEDYDYIIIGGGTA 63

Query: 61  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVEN 120
           GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFV EDDG+NPFQ+F+SEDGVE 
Sbjct: 64  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSEDGVEI 123

Query: 121 IRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSA 180
           IRGRVLGGGSM NAGFYSRGH EFFESAGVDWDME+VEK YEWVE+TVVS+P LSAWQSA
Sbjct: 124 IRGRVLGGGSMVNAGFYSRGHGEFFESAGVDWDMELVEKGYEWVEKTVVSRPRLSAWQSA 183

Query: 181 FRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATV 240
           FR ALLEGGVG DNGFDLRH VGTKTGGSIFD  GNRHGAVELLN  EP NLKVA +ATV
Sbjct: 184 FRRALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATV 243

Query: 241 QKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           QKIIFS LSA GVSYSDSKGK HTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL
Sbjct: 244 QKIIFSDLSAIGVSYSDSKGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 303

Query: 301 KLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAP 360
           KLPVVL QPHVGEFMSDNPRFS  IVLPF LVASSAQVVG LD NI+LQ+FASP PFF  
Sbjct: 304 KLPVVLHQPHVGEFMSDNPRFSAAIVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFIL 363

Query: 361 PSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLAR 420
           P+F LLPPQST I PS+A+ +GKF+DV S+GSL LNSSTDVKNNPIVRFNYYS+P+DLA+
Sbjct: 364 PTFGLLPPQSTLINPSLALLIGKFTDVLSKGSLHLNSSTDVKNNPIVRFNYYSNPNDLAQ 423

Query: 421 CVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYW 480
           CVRG+RKVGDLLKT+ MEK KT+DLEGNKGFLFLG PLPENL D SSVEEYC++TV TYW
Sbjct: 424 CVRGLRKVGDLLKTRMMEKIKTRDLEGNKGFLFLGPPLPENLSDISSVEEYCKETVTTYW 483

Query: 481 HYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRS 540
           HYHGGCLVGKVVD N++VIG+ENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL +LQ+RS
Sbjct: 484 HYHGGCLVGKVVDGNYRVIGVENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERS 543

Query: 541 S 542
           S
Sbjct: 544 S 544

BLAST of Lsi11G007440 vs. ExPASy TrEMBL
Match: A0A6J1EP46 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111436113 PE=3 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 2.0e-260
Identity = 444/541 (82.07%), Postives = 490/541 (90.57%), Query Frame = 0

Query: 1   MEHSMATILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTA 60
           ME S+ATIL  ILIS FQ G +SS+ IP+QD SYMKFVH A +LPEK+EYDYIIIGGG A
Sbjct: 1   MERSIATILFLILISTFQFGAISSNTIPDQDVSYMKFVHNAGDLPEKQEYDYIIIGGGAA 60

Query: 61  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVEN 120
           GCPLAATLSSKF VLLLERGS+PNKYPSVLNEQGLLN F+A+DDGQNPFQ+F SEDGVEN
Sbjct: 61  GCPLAATLSSKFKVLLLERGSEPNKYPSVLNEQGLLNAFLAQDDGQNPFQRFTSEDGVEN 120

Query: 121 IRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSA 180
           IRGR+LGG +M NAGFYSRGHR+FFE+AGV+WDMEMVE AY+WVEETVVSKP L+AWQSA
Sbjct: 121 IRGRILGGSTMVNAGFYSRGHRQFFETAGVNWDMEMVENAYQWVEETVVSKPILNAWQSA 180

Query: 181 FRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATV 240
           F+SALLE GVG DNGF+L H +GTKTGGSIFD KGNRHGAVELLNKAEP NLKVA  ATV
Sbjct: 181 FKSALLEAGVGPDNGFNLTHLIGTKTGGSIFDGKGNRHGAVELLNKAEPMNLKVAVNATV 240

Query: 241 QKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           ++I+F+GLSANGVSYSDSKGK+HTAFIRKKGEI LSAGAIGSP LLL SGVGPKSHLSSL
Sbjct: 241 RQILFNGLSANGVSYSDSKGKIHTAFIRKKGEIFLSAGAIGSPLLLLQSGVGPKSHLSSL 300

Query: 301 KLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAP 360
           KLPVV  QP+VGEFMSDNPRF  TIVLPFQL +SS +VVGTL +N++LQ+FASP PF AP
Sbjct: 301 KLPVVHHQPYVGEFMSDNPRFGATIVLPFQLASSSVKVVGTLQDNVHLQAFASPAPFLAP 360

Query: 361 PSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLAR 420
           P+FSLLPPQ+TSI PS+ IFVGKFS+VHSEG LRLNS+TD KNN IVRFNYYSHPDDLAR
Sbjct: 361 PTFSLLPPQATSINPSLVIFVGKFSEVHSEGFLRLNSTTDAKNNVIVRFNYYSHPDDLAR 420

Query: 421 CVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYW 480
           CV GVRKVGDLLKTQTMEK KTQDLEGNKGF F+G+PLPENL DDSSVEEYC+KTV TYW
Sbjct: 421 CVEGVRKVGDLLKTQTMEKIKTQDLEGNKGFQFMGVPLPENLGDDSSVEEYCRKTVTTYW 480

Query: 481 HYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRS 540
           HYHGGCLVGKVVD +++VIG++NLRVVDGSTFS SPGTNPMATLMMLGRYVGL VLQ+RS
Sbjct: 481 HYHGGCLVGKVVDGDYRVIGMKNLRVVDGSTFSDSPGTNPMATLMMLGRYVGLKVLQERS 540

Query: 541 S 542
           S
Sbjct: 541 S 541

BLAST of Lsi11G007440 vs. NCBI nr
Match: XP_008451922.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa] >TYK10909.1 (R)-mandelonitrile lyase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 981.1 bits (2535), Expect = 3.9e-282
Identity = 489/534 (91.57%), Postives = 510/534 (95.51%), Query Frame = 0

Query: 8   ILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPLAAT 67
           ILLF LISIFQLGV+SSH IPNQDTSYMKFVHEASEL E EEYDYIIIGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 68  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIRGRVLG 127
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLN FVAEDDGQNPFQ FISEDGVEN+RGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 128 GGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSALLE 187
           GGSM NAGFYSRGHREFFE+AGVDWDME+VEKAYEWVEETVVSKPSLS WQ+AFRSALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 188 GGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKIIFSG 247
           GGVG DNGFDL+H VGTKTGGSIFDNKGNRHGAVELLNK EP+N KVATQATVQ+IIF+G
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 248 LSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLD 307
           LSA+GVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SLKLPVVLD
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 308 QPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAPPSFSLLP 367
           QPHVGEFMSDNPRF+PTIVLPFQ+VASSAQVVGTLDNNI+LQ+FASPLPFFAPPSFSLLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 368 PQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVRK 427
           PQSTSIVPS+AIFVGKFSDV+SEGSLRLNSS DVK +PIVRFNYYSHPDDLARCVRGVRK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 428 VGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYWHYHGGCL 487
           VGDLL+T TMEK KTQDLEGNK F FLGL LPENL +DS+VEEYCQKTV TYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 488 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRSS 542
           VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL VLQQRSS
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

BLAST of Lsi11G007440 vs. NCBI nr
Match: XP_038889699.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 980.3 bits (2533), Expect = 6.6e-282
Identity = 489/541 (90.39%), Postives = 512/541 (94.64%), Query Frame = 0

Query: 1   MEHSMATILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTA 60
           M+ SMATILL ILISIFQLGV SSH IP+QDTSY+KFVHEA+ELPE  EYDYIIIGGGTA
Sbjct: 1   MKRSMATILLIILISIFQLGVFSSHTIPDQDTSYLKFVHEANELPETGEYDYIIIGGGTA 60

Query: 61  GCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVEN 120
           GCPLAATLSSK+SVLLLERGSDPNKYPSVLNEQGLLN FVAEDDGQNPFQ FISEDGVEN
Sbjct: 61  GCPLAATLSSKYSVLLLERGSDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVEN 120

Query: 121 IRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSA 180
           IRGRVLGGGSM NAGFYSRGHREFFE+AGVDWDME+VEKAY+WVE+TVVS+PSLSAWQSA
Sbjct: 121 IRGRVLGGGSMINAGFYSRGHREFFETAGVDWDMELVEKAYQWVEQTVVSRPSLSAWQSA 180

Query: 181 FRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATV 240
           FR ALLE GVG DNGFDL+H VGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKV TQA V
Sbjct: 181 FRGALLEAGVGPDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVGTQAIV 240

Query: 241 QKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSL 300
           ++IIF+GLSA+GVSYSD KGKLHTAFIR+KGEIILSAGAIGSPQLLLLSGVGPK HLSSL
Sbjct: 241 ERIIFTGLSASGVSYSDPKGKLHTAFIREKGEIILSAGAIGSPQLLLLSGVGPKYHLSSL 300

Query: 301 KLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFAP 360
           KLPVVL QPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTL NNI+LQSFASPLPFFAP
Sbjct: 301 KLPVVLHQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLANNIHLQSFASPLPFFAP 360

Query: 361 PSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLAR 420
           PSFSLLPPQSTSI+PS+AIFVGKFSDV SEGSLRLNSS DVK +PIVRFNYYS+ DDLAR
Sbjct: 361 PSFSLLPPQSTSIIPSLAIFVGKFSDVLSEGSLRLNSSIDVKESPIVRFNYYSNHDDLAR 420

Query: 421 CVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTYW 480
           CVRGVRKVGDLLKTQTMEK K QDLEGNKGF FLGLPLPENLW+DS+VEEYCQKTV TYW
Sbjct: 421 CVRGVRKVGDLLKTQTMEKIKNQDLEGNKGFQFLGLPLPENLWNDSAVEEYCQKTVTTYW 480

Query: 481 HYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQRS 540
           HYHGGCLVGKVVD NHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL VLQQRS
Sbjct: 481 HYHGGCLVGKVVDHNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRS 540

Query: 541 S 542
           S
Sbjct: 541 S 541

BLAST of Lsi11G007440 vs. NCBI nr
Match: XP_031736478.1 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical protein Csa_021176 [Cucumis sativus])

HSP 1 Score: 976.5 bits (2523), Expect = 9.5e-281
Identity = 488/542 (90.04%), Postives = 516/542 (95.20%), Query Frame = 0

Query: 1   MEHSMAT-ILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGT 60
           M++SMA  ILLF LISIFQLG+++SH IPNQDTSYMKFV+EASEL E EEYDYIIIGGGT
Sbjct: 1   MKNSMANQILLFTLISIFQLGLIASHTIPNQDTSYMKFVYEASELQESEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVE 120
           AGCPLAATLSSKFSVLLLERG+DPNKYPSVLNEQGLLN FVAEDDGQNPFQ FISEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVE 120

Query: 121 NIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQS 180
           N+RGRVLGGGSM NAGFYSRGHR+FFE+AGVDWD E+VEKAYEWVEETVVSKPSLS WQ+
Sbjct: 121 NLRGRVLGGGSMINAGFYSRGHRDFFETAGVDWDRELVEKAYEWVEETVVSKPSLSPWQA 180

Query: 181 AFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQAT 240
           AFRSALLEGGVG D GFDLRH VGTKTGGSIFDNKGNRHGAVELLNK EP+NLKVATQAT
Sbjct: 181 AFRSALLEGGVGHDKGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKGEPKNLKVATQAT 240

Query: 241 VQKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS 300
           VQ+IIF+GLSA+GVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQRIIFTGLSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSYLSS 300

Query: 301 LKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFA 360
           L+LPVVLDQPHVGEFMSDNPRFSPTIVLPFQLV+SSAQVVGTLD+NI+LQSFASPLPFFA
Sbjct: 301 LRLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVSSSAQVVGTLDHNIHLQSFASPLPFFA 360

Query: 361 PPSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ TSIVPS+AIFVGKFSDVHSEGSLRLNSS DVK +PIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFTSIVPSLAIFVGKFSDVHSEGSLRLNSSIDVKESPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTY 480
           RCVRGVRKVGDLLKT TMEK KTQDLEGNK F FLGL LPENL +D++VEEYCQKTV TY
Sbjct: 421 RCVRGVRKVGDLLKTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDTAVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQR 540
           WHYHGGCLVGKVVDDNHKVIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGL +LQQR
Sbjct: 481 WHYHGGCLVGKVVDDNHKVIGIDNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKLLQQR 540

Query: 541 SS 542
           SS
Sbjct: 541 SS 542

BLAST of Lsi11G007440 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 920.2 bits (2377), Expect = 8.1e-264
Identity = 455/542 (83.95%), Postives = 493/542 (90.96%), Query Frame = 0

Query: 1   MEHSMATIL-LFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGT 60
           MEHS +TIL L ++ISIF LG+LSSH IPNQD  YMKFVH A++LP KEEYDYIIIGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVE 120
           AGCPL++TLSSKFSVLLLERGSDPNKYPSVLNEQGLLN F AEDDG+NPFQ FISEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 121 NIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQS 180
           NIRGRVLGGGSM N GFYSRGH++FFE+AGVDWDME+VEKAY+WVEETVVS+P L+AWQS
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 181 AFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQAT 240
           AFRSA L+ GV  DNGFDLRH VGTK GGSIFDNKGNRHGAVELLNKA P N+KV  +AT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 241 VQKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS 300
           VQ+I+FSGLSA GV YSDSKGKLH AFIRK+GEII+SAGA+GSPQLLLLSGVGPKSHLSS
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 301 LKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFA 360
           LKLPVVL QP+VGEFMSDNPRF   I+LPFQLV SS +VVG L +NIY+QSFASP PFFA
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ  SIVPS+A+FVGKFS+VHSEGSLRLNSST+VK +PIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTY 480
           RCV+GVRK+GDLLKTQTMEK KT DLEGNKGF FLGLPLPENL +DSSVEEYCQKTV TY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQR 540
           WHYHGGCLVGKVVD N+KVIGIENLRVVDGSTF  SPGTNPMATLMMLGRYVGL VLQQR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SS 542
           SS
Sbjct: 541 SS 542

BLAST of Lsi11G007440 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 917.1 bits (2369), Expect = 6.9e-263
Identity = 459/542 (84.69%), Postives = 496/542 (91.51%), Query Frame = 0

Query: 1   MEHSMATIL-LFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGT 60
           MEHS ATIL L ++ISI QLGVL+ + IP +D  YMKFVH+AS+LP KEEYDYIIIGGGT
Sbjct: 1   MEHSKATILILSLMISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGGT 60

Query: 61  AGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVE 120
           AGCPLAATLSSKFSVL+LERGSDPNKYPSVLNEQGLLNVF A DDG+NPFQ+FISEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGVE 120

Query: 121 NIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQS 180
           NIR RVLGGGSM NAGFYSRGH++FFE+AGVDWDME+VEKAY+WVEETV S+P L+AWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQS 180

Query: 181 AFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQAT 240
           AFRSALLE GV  DNGFDLRH VGTKTGGSIFD+KGNRHGAVELLNKAEP N+KVA +AT
Sbjct: 181 AFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEAT 240

Query: 241 VQKIIFSGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSS 300
           VQKI+FSGLSA GV YSDSKGKLH AFIRKKGEII+SAGAIGSPQLLLLSGVGPKSHLSS
Sbjct: 241 VQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLSS 300

Query: 301 LKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASPLPFFA 360
           LKLPVVL QP+VGEFMSDNPRF   I+LPFQLV SS +VVG L +NIY+QSFASP PFFA
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 361 PPSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLA 420
           PPSFSLLPPQ  SIVPS+A+FVGKFS+VHSEGSLRLNSST+VK +PIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 421 RCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQKTVMTY 480
           RCV+GVRK+GDLLKTQTMEK KT DLEGNKGF FLGLPLPENL +DSSV EYCQKTV TY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTTY 480

Query: 481 WHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQR 540
           WHYHGGCLVGKVVD N+KVIGI+NLRVVDGSTFS SPGTNPMATLMMLGRYVGL VLQQR
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKVLQQR 540

Query: 541 SS 542
           SS
Sbjct: 541 SS 542

BLAST of Lsi11G007440 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 1.1e-149
Identity = 270/546 (49.45%), Postives = 369/546 (67.58%), Query Frame = 0

Query: 6   ATILLFILISIFQLGVLSSHNI---PNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGC 65
           +++L   L+ +  LGV+   N     N+   +M+F+  A++   ++ YDYII+GGGTAGC
Sbjct: 7   SSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGC 66

Query: 66  PLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIR 125
           PLAATLS  F VLLLERG  P   P+V++  G L      ++  +P Q FISE+GV N R
Sbjct: 67  PLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNAR 126

Query: 126 GRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFR 185
           GRVLGG S  NAGFYSR  ++FFE++G+ WD+  V ++YEWVE  +V +P L  WQ+A R
Sbjct: 127 GRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIR 186

Query: 186 SALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQK 245
            ALLE GV   NGF L H VGTK GGS FD  G RH + +LL  A   N++VA  ATV++
Sbjct: 187 DALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVER 246

Query: 246 IIF--------SGLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPK 305
           ++         S +SA GV Y D  G+ H A IR +GE+ILSAGA+GSPQLL LSG+GP+
Sbjct: 247 VLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPR 306

Query: 306 SHLSSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQSFASP 365
           S+LS+  +PV LDQPHVG+F+ DNPR   +IV P  +  S  QVVG  ++  +L++ ++ 
Sbjct: 307 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 366

Query: 366 LPFFAP-PSFSLLPPQSTSIVPSVAIFVGKFSDVHSEGSLRLNSSTDVKNNPIVRFNYYS 425
           +PF +P  S  +  P S   VP V   + K     S G LRL +STDV+ NP+VRFNY+S
Sbjct: 367 IPFASPLHSVFIRAPASPLYVP-VTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFS 426

Query: 426 HPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLPENLWDDSSVEEYCQ 485
            P DL RCV G RK+G++L+++ M+ F  ++  GN+ F F+G PLP +  +D  + ++C+
Sbjct: 427 DPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCR 486

Query: 486 KTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGL 540
           +TV T WHYHGG +VGKVVD + KVIG+ +LR+VDGSTF++SPGTNP ATLMMLGRY+GL
Sbjct: 487 RTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGL 546

BLAST of Lsi11G007440 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.3e-115
Identity = 241/569 (42.36%), Postives = 330/569 (58.00%), Query Frame = 0

Query: 10  LFILISIF-QLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPLAATL 69
           LF+ +S+F    + SS   PN       F+ +A+  P    YDYIIIGGGTAGCPLAATL
Sbjct: 10  LFVALSLFLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATL 69

Query: 70  SSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIRGRVLGG 129
           S   SVLLLERG  P   P++          +++    +P Q+F+SEDGV N R RVLGG
Sbjct: 70  SQNASVLLLERGDSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGG 129

Query: 130 GSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSALLEG 189
           GS  NAGFY+R   ++  + G  WD  +  ++Y+WVE  V  +P +  WQ+A R  LLE 
Sbjct: 130 GSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEA 189

Query: 190 GVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKIIFSGL 249
           G+  +NGF   H  GTK GG+IFD  GNRH A +LL  A+P+ + V   ATV +I+F   
Sbjct: 190 GIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTR 249

Query: 250 S-----ANGVSYSDSKGKLHTAFIRK--KGEIILSAGAIGSPQLLLLSGVGPKSHLSSLK 309
                 ANGV Y D  G+ H A++++    EIILSAG +GSPQLL+LSGVGP + L +  
Sbjct: 250 GTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQN 309

Query: 310 LPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQS----------- 369
           + VV+DQPHVG+ M DNP  +  +  P  +  S  +VVG      Y+++           
Sbjct: 310 ITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGG 369

Query: 370 ----FASPLPFFA--PPSFSLLPPQSTSIVPSVAIFVG-----KFSDVHSEGSLRLNSST 429
                +S   ++A   P  +LL   S + + S   F G     K     S G L L  + 
Sbjct: 370 GSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTR 429

Query: 430 DVKNNPIVRFNYYSHPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPLP 489
           + K+NPIV FNY+ HPDDL RCVRG++ +  +++++   ++K  D+   +  L L    P
Sbjct: 430 NPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV-SFEYLLNLTASTP 489

Query: 490 ENLWD---------DSSVEEYCQKTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVDGS 540
            NL             S EE+CQ TV T WHYHGGC+VG+VVD ++KVIGI+ LRV+D S
Sbjct: 490 VNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMS 549

BLAST of Lsi11G007440 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 6.9e-112
Identity = 235/538 (43.68%), Postives = 307/538 (57.06%), Query Frame = 0

Query: 46  EKEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNVFVAED 105
           +   YDYI+IGGGTAGCPLAATLS  FSVL+LERG  P  N   S L      ++ +A+ 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 106 DGQNPFQQFISEDGVENIRGRVLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEW 165
              +  Q F+S DGV N R RVLGGGS  NAGFYSR    F + AG  WD ++V+++Y W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 166 VEETVVSKPSLSAWQSAFRSALLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVEL 225
           VE  +V +P L+ WQ A R +LLE GV   NGF   H  GTK GG+IFD  G RH A EL
Sbjct: 179 VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 226 LNKAEPQNLKVATQATVQKIIF--SGL--SANGVSYSDSKGKLHTAFI--RKKGEIILSA 285
           L  A PQ L+V   ATVQKI+F  SG      GV + D KG  H A +  RK  E+ILS+
Sbjct: 239 LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 286 GAIGSPQLLLLSGVGPKSHLSSLKLPVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQ 345
           GAIGSPQ+L+LSG+GPK  L  LK+PVVL+  HVG+ M+DNP  +  +     +  S  Q
Sbjct: 299 GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 346 VVGTLDNNIYLQSFASPLPFFAPPS---------------FSLLPPQSTSIVPSVAIFVG 405
            VG     +Y+++      F   P                FS +P +      + A    
Sbjct: 359 TVGITKMGVYVEASTG---FGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITR 418

Query: 406 KFSDVH----------------SEGSLRLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVR 465
               +H                S G L L  +T+V +NP V FNY+ HP DL RCV  +R
Sbjct: 419 NKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIR 478

Query: 466 KVGDLLKTQTMEKFKTQDLEGNKGFLFLGLPL-----PENLWDDSSVEEYCQKTVMTYWH 525
            V  ++ +     +   D +     L L +       P+ L D  S+ ++C+ TV+T WH
Sbjct: 479 LVSKVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWH 538

Query: 526 YHGGCLVGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLTVLQQR 540
           YHGGCLVGKVV  N KV+G++ LRV+DGSTF  SPGTNP AT+MM+GRY+G+ +L++R
Sbjct: 539 YHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

BLAST of Lsi11G007440 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.4e-109
Identity = 245/571 (42.91%), Postives = 328/571 (57.44%), Query Frame = 0

Query: 11  FILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPLAATLSS 70
           FIL+++F    +   +  + +    +F+ +A+  P+   +DYIIIGGGTAGC LAATLS 
Sbjct: 11  FILVAVF----IFHDSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQ 70

Query: 71  KFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQ-FISEDGVENIRGRVLGGG 130
             +VL+LERG  P   P+  +     N  +  +   N + Q FISEDGV N R RVLGGG
Sbjct: 71  NATVLVLERGGSPYDDPAATDIGNFANTLL--NITPNSWSQLFISEDGVFNSRARVLGGG 130

Query: 131 SMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSALLEGG 190
           ++ NAGFYSR   +F   AG  W+ + VE AYEWVE+ VV +P ++ WQSAFR  LLE G
Sbjct: 131 TVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAG 190

Query: 191 VGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKIIFS--- 250
           V   NGF   H VGTK GG+IFD  G+RH A  LL  A P  + V   A+V KI+F+   
Sbjct: 191 VTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKG 250

Query: 251 ----------GLSANGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSH 310
                      L ANGVSY   K +L T       E+ILSAGAI SPQLL+LSGVGP +H
Sbjct: 251 NQRPKAYGVIFLDANGVSY---KAELATQ-DSTMSEVILSAGAIASPQLLMLSGVGPAAH 310

Query: 311 LSSLKL-PVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQ-SFASP 370
           L++ ++ PV++DQP VG+ M DNP     I  P  +  S  Q VG      Y++   A  
Sbjct: 311 LAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALS 370

Query: 371 LPFFAPPSF----------SLLPPQSTS-----------IVPSVAIFVGKFSDVHSEGSL 430
           L      SF          + LP QS S           +     + + K +   S G L
Sbjct: 371 LSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHL 430

Query: 431 RLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVRKVGDLLKTQTMEKFKTQDLEGNKGFLF 490
            L  +T+  +NP V FNY+  P+DL +CV G+  +  ++ ++   K+K   L   +G L 
Sbjct: 431 ELR-NTNPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARGLLN 490

Query: 491 LGLPLPENL-----WDDSSVEEYCQKTVMTYWHYHGGCLVGKVVDDNHKVIGIENLRVVD 540
           L L LP NL          +E+YC  TVMT +HYHGGC VGKVVD+N+KV+G++ LR++D
Sbjct: 491 LILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALRIID 550

BLAST of Lsi11G007440 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 387.1 bits (993), Expect = 2.3e-107
Identity = 237/581 (40.79%), Postives = 326/581 (56.11%), Query Frame = 0

Query: 5   MATILLFILISIFQLGVLSSHNIPNQDTSYMKFVHEASELPEKEEYDYIIIGGGTAGCPL 64
           M   +L++   IF +  +   +       Y  F+ +A+  P    +DYIIIGGGT+GC L
Sbjct: 1   MGLQILWLFRKIFIITYIFHASCSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCAL 60

Query: 65  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVAEDDGQNPFQQFISEDGVENIRGR 124
           AATLS   SVL+LERG  P   P+  + +      ++    ++  Q FISEDGV N R R
Sbjct: 61  AATLSQNASVLVLERGGAPYDNPTATDIENFATT-LSNTSPKSWSQLFISEDGVYNTRAR 120

Query: 125 VLGGGSMANAGFYSRGHREFFESAGVDWDMEMVEKAYEWVEETVVSKPSLSAWQSAFRSA 184
           VLGGGS+ NAGFY+R   E+ +    +W  + VE AYEWVE+ V  +P +  WQ+AF+  
Sbjct: 121 VLGGGSVLNAGFYTRAGDEYVKE--TEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDG 180

Query: 185 LLEGGVGADNGFDLRHHVGTKTGGSIFDNKGNRHGAVELLNKAEPQNLKVATQATVQKII 244
           LLE G    NGF   H  GTK GG+IFD  G+RH A +LL  A P N+ V   A+V KI+
Sbjct: 181 LLEAGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKIL 240

Query: 245 FSGL-----SANGVSYSDSKGKLHTAFIRKK--GEIILSAGAIGSPQLLLLSGVGPKSHL 304
           F+        A GV + D+ G LH A + K    E+ILSAGAIGSPQLL+LSG+GP +HL
Sbjct: 241 FTTKGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHL 300

Query: 305 SSLKL-PVVLDQPHVGEFMSDNPRFSPTIVLPFQLVASSAQVVGTLDNNIYLQ------- 364
           ++  + P+VLD P VG+ M DNP  +  I  P  +  S  QVVG      Y++       
Sbjct: 301 AAHGIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIF 360

Query: 365 SFASPLPFF------------APPSFSLLPPQSTSIVPS--------------VAIFVGK 424
           S++    FF            +  + +  P  ST  +                  + + K
Sbjct: 361 SYSWTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQK 420

Query: 425 FSDVHSEGSLRLNSSTDVKNNPIVRFNYYSHPDDLARCVRGVRKVGDLLKTQTMEKFKTQ 484
            +   S G L L  +T+  +NP VRFNYY  P+DL  CV G+  +  ++ ++   KFK  
Sbjct: 421 IAGPISRGHLELR-NTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYP 480

Query: 485 DLEGNKGFLFLGLPLPENL-----WDDSSVEEYCQKTVMTYWHYHGGCLVGKVVDDNHKV 540
           D   + G L L L +P NL         ++ ++C  TVMT WHYHGGC VG+VVD N++V
Sbjct: 481 DATIH-GLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRV 540

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527064.5e-15651.01(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K22.1e-15350.28(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527071.9e-15149.54(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O242439.0e-14949.54(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Q9SSM21.5e-14849.45(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3CL001.9e-28291.57(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BSP41.9e-28291.57(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=... [more]
A0A0A0LK794.6e-28190.04Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055050 PE=3 SV=1[more]
A0A6J1FC902.8e-26284.84(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE... [more]
A0A6J1EP462.0e-26082.07(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_008451922.13.9e-28291.57PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo] >KAA0067298.1 (R)-mand... [more]
XP_038889699.16.6e-28290.39(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_031736478.19.5e-28190.04(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61117.1 hypothetical prote... [more]
XP_038889315.18.1e-26483.95(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_038889314.16.9e-26384.69(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G73050.11.1e-14949.45Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.12.3e-11542.36Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.16.9e-11243.68Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.11.4e-10942.91Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.12.3e-10740.79Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 29..352
e-value: 7.3E-61
score: 204.6
coord: 355..541
e-value: 5.9E-31
score: 105.8
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 388..529
e-value: 1.1E-28
score: 100.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 50..318
e-value: 1.6E-34
score: 119.7
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 278..292
NoneNo IPR availableGENE3D3.30.410.40coord: 170..478
e-value: 9.5E-172
score: 574.3
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 5..540
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 5..540
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 388..485
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 50..528
e-value: 9.5E-172
score: 574.3
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 37..538

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G007440.1Lsi11G007440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors