Lsi11G007420 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G007420
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	(R)-mandelonitrile lyase 1-like
Location	chr11: 8136907 .. 8139446 (-)
RNA-Seq Expression	Lsi11G007420
Synteny	Lsi11G007420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGAGAACGGCAGGCTGCCCATTAGCTGCAACTTTATCATCAAAATTCTCAATCCTCCTTCTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTTTGATGAACGCTTTTGTTGAAGAAGATGATGGCAAAAATCCCTTCCAACGCTTCATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGGGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGGCATCGACAGTTTTTTGAAAGAGCAGGTGTTGATTGGGAGATGGAATTGGTGGAGAAGGCTTATGAATGGGTTGAAGAGACTGTAGTATCTCGACCAATTATTTTAAAAGCTTGGCCAGCTGCTTTTAGAAGTGCTTTGTTGGAAGGTGGTGTTGCACCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGAACTAAAATTGGTGGACCTGATAAGGAAACAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCTAAAAACCTTCAAGTTGCAATTCAAGCCACAATCCAGAAATTCCTATTCTCTGGTTAGTCCTTGTAAATATTTGGTTTTAATTCTGTTTCAAGACTTAAATTTTAGAAAATAAATCATTTTAATTTGGCTAAATTATAAAAAAATGACCGTAAATTTTGTACTTTCTATCCAAAAAAAAAAAATACTTCTGAAGTTTCAAAACTTTAAAAAATACTCTTAAAACTATCAAGAAGATCAAAAATACATGTGCTATTAATTTTGGATGGAAACCATTAAAGTTTTGTTTAAAAAATATCTATGATTCATGAACTTAAAAAAGTTAAAAAATACCTTCATTGTTAGTATATGAACAGAAACTGTTAATACCTCGTTAGTTAGAAAATACTTTCATCATTTGGATGATTTTTTTTGTCCCTTTATTCTTCCCCGGAGAAGAAAATCAAAAAGTAATTATTTTTTTTTTGGTCAACCAGAAAGCATAAAACTAAAAATATATATAGCTCATAATTAGAAAAGAAAAGGATTGAATCAATAAATTAAAGGAAAAAAAGTATTATTTTTTTTGTCGAAATCAATGAACATGAAAGAGAAAAAAAAATGATGGAAAATGCCCCCATAGTTAAACTATAAAAACTTGTTAGGAATAGAAAAGTTGTACTTGAAAAGATTGTTATCACCTAAAAAGCAAAAACAAAAATACAATAGCCGGACAAAATATTGAAACTAACATTGAATTTGAGATAGCTGATTAAACATAAGTTGTTTTTAAATATAGCCAAATGAGTTAATTTATTTACAAATATAGTAAAATAATATCATCTATCCATGATAGACACTAATAAACACCGATATACATCTATTAGTGTCAAACTTATAGATGACACTGATAGATGTCTATTCGTGTCTATCCGTGTATATCAACAATAACATTTTGTTATATTTGTAAATATTTTCAGCAATTTTACCATTTAAAATAATTACCCTTCAACAACTCGAGTTTGCTAAGGTAATGTACGTGTAAATTTAATAAATAATAATTTAAAATCTTTCTTCTTTTATTTTTTATTTTCTAGTTTTTTTATCCCAACACATGAAATGATTTCAAATCAAGGGGATCCAAATAGGGAGTTAATTTTATTACATTGAAGCACTTAATTTTTCATATTGTTTCACATGCATTAGGTTTATCTGCACGTGGGGTTTCCTATTCCGATTCAAAAGGAAATTTGCATACAGCATTCATTCGCAAGAAAGGTGAAATCATTCTAAGTGCTGGAGCCATTGGAAGCTCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATTCATGTCCGACAATCCCCGTTTTGGGACCAACATCGTTCTTCCATTCCAATTGGTTCCTTTGTCCGGAAAAGTTGTTGGAATTTTAGAAGACAATATATATTTCCAATCTCTTGCAGGCCCTTCACCATTTTTAGTTCCACCAACCTTCAGTCTTCTTCCTCCTCATTCCACTTCAATTAATCCTAGCTTAGCCACTTTTTTTGGGAAATTCTCAGAGGTGCATTCTGAAGGGTCGCTTCGATTGAATTCCTCCATTGATGTAAAGAAGAGTCCCATTGTTCAATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGCGTTAGAGGAGTAAGAAAATTTGGAGATTTGCTTAAAACTCCAACAATGGAAAAAATTAAGACCCAAGATTTGGAGGGTAACAAAGGATTTCTGTTTTTTGGGGTTTCCCTTGCCGGATAATTTGTGGAATGATAGCTCTGTTCAAGAATACTGTAAAAACACTGTGGCTACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGATAATTACAGAGTCATCGGAATTGAAAATTTGCGTGTAGTGGATGGCTCCACTTTCTCTATCTCACCGGGAACAAATCCTATGGCCACACTCATGATGCTCGGC

mRNA sequence

TGGAGAACGGCAGGCTGCCCATTAGCTGCAACTTTATCATCAAAATTCTCAATCCTCCTTCTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTTTGATGAACGCTTTTGTTGAAGAAGATGATGGCAAAAATCCCTTCCAACGCTTCATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGGGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGGCATCGACAGTTTTTTGAAAGAGCAGGTGTTGATTGGGAGATGGAATTGGTGGAGAAGGCTTATGAATGGGTTGAAGAGACTGTAGTATCTCGACCAATTATTTTAAAAGCTTGGCCAGCTGCTTTTAGAAGTGCTTTGTTGGAAGGTGGTGTTGCACCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGAACTAAAATTGGTGGACCTGATAAGGAAACAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCTAAAAACCTTCAAGTTGCAATTCAAGCCACAATCCAGAAATTCCTATTCTCTGGTTAGTTTATCTGCACGTGGGGTTTCCTATTCCGATTCAAAAGGAAATTTGCATACAGCATTCATTCGCAAGAAAGGTGAAATCATTCTAAGTGCTGGAGCCATTGGAAGCTCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATTCATGTCCGACAATCCCCGTTTTGGGACCAACATCGTTCTTCCATTCCAATTGGTTCCTTTGTCCGGAAAAGTTGTTGGAATTTTAGAAGACAATATATATTTCCAATCTCTTGCAGGCCCTTCACCATTTTTAGTTCCACCAACCTTCAGTCTTCTTCCTCCTCATTCCACTTCAATTAATCCTAGCTTAGCCACTTTTTTTGGGAAATTCTCAGAGGTGCATTCTGAAGGGTCGCTTCGATTGAATTCCTCCATTGATGTAAAGAAGAGTCCCATTGTTCAATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGCGTTAGAGGAGTAAGAAAATTTGGAGATTTGCTTAAAACTCCAACAATGGAAAAAATTAAGACCCAAGATTTGGAGGAATACTGTAAAAACACTGTGGCTACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGATAATTACAGAGTCATCGGAATTGAAAATTTGCGTGTAGTGGATGGCTCCACTTTCTCTATCTCACCGGGAACAAATCCTATGGCCACACTCATGATGCTCGGC

Coding sequence (CDS)

TGGAGAACGGCAGGCTGCCCATTAGCTGCAACTTTATCATCAAAATTCTCAATCCTCCTTCTAGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTTTGATGAACGCTTTTGTTGAAGAAGATGATGGCAAAAATCCCTTCCAACGCTTCATCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGGGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGGCATCGACAGTTTTTTGAAAGAGCAGGTGTTGATTGGGAGATGGAATTGGTGGAGAAGGCTTATGAATGGGTTGAAGAGACTGTAGTATCTCGACCAATTATTTTAAAAGCTTGGCCAGCTGCTTTTAGAAGTGCTTTGTTGGAAGGTGGTGTTGCACCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGAACTAAAATTGGTGGACCTGATAAGGAAACAGACATGGAGCTGTGGAGCTTCTTAATAAGGCTAATCCTAAAAACCTTCAAGTTGCAATTCAAGCCACAATCCAGAAATTCCTATTCTCTGGTTAGTTTATCTGCACGTGGGGTTTCCTATTCCGATTCAAAAGGAAATTTGCATACAGCATTCATTCGCAAGAAAGGTGAAATCATTCTAAGTGCTGGAGCCATTGGAAGCTCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCATCTTTAAAACTACCTGTCGTTCTTCACCAACCACATGTCGGCCAATTCATGTCCGACAATCCCCGTTTTGGGACCAACATCGTTCTTCCATTCCAATTGGTTCCTTTGTCCGGAAAAGTTGTTGGAATTTTAGAAGACAATATATATTTCCAATCTCTTGCAGGCCCTTCACCATTTTTAGTTCCACCAACCTTCAGTCTTCTTCCTCCTCATTCCACTTCAATTAATCCTAGCTTAGCCACTTTTTTTGGGAAATTCTCAGAGGTGCATTCTGAAGGGTCGCTTCGATTGAATTCCTCCATTGATGTAAAGAAGAGTCCCATTGTTCAATTCAATTATTATTCACATCCTGATGATCTTGCTCGATGCGTTAGAGGAGTAAGAAAATTTGGAGATTTGCTTAAAACTCCAACAATGGAAAAAATTAAGACCCAAGATTTGGAGGAATACTGTAAAAACACTGTGGCTACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGATAATTACAGAGTCATCGGAATTGAAAATTTGCGTGTAGTGGATGGCTCCACTTTCTCTATCTCACCGGGAACAAATCCTATGGCCACACTCATGATGCTCGGC

Protein sequence

WRTAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAWPAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPDKETDMELWSFLIRLILKTFKLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSPFLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPDDLARCVRGVRKFGDLLKTPTMEKIKTQDLEEYCKNTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG

Homology

[Close]

BLAST of Lsi11G007420 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.2e-111
Identity = 220/475 (46.32%), Postives = 294/475 (61.89%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           T+GCPLAATLS K+ +L+LERGS P  YP+VL   G +    +EDDGK P +RF+SEDG+
Sbjct: 63  TSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGI 122

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           +N+RGRVLGG SM+NAG Y+R +   +  +GVDW+M+LV K YEWVE+T+V +P   + W
Sbjct: 123 DNVRGRVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKP-NYQPW 182

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            +   +A LE GV P++GF L H  GT+I G    +K T       L +      ++   
Sbjct: 183 QSVTGTAFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVH 242

Query: 183 PQSRNSY--SLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPK 242
                    +   L+A GV Y DS G  H AF+R KGE+I+SAG IG+ QLLLLSGVGP+
Sbjct: 243 ASVEKIIFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPE 302

Query: 243 SHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGP 302
           S+LSSL +PVVL  P+VGQF+ DNPR   NI+ P  + P    V+GI  D  ++Q     
Sbjct: 303 SYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSS 362

Query: 303 SPFLVPPTFSLLPPHSTSI-NPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYS 362
            PF  PP FS  P  S  + N + A F  K +   S GSL L SS +V+ SP V+FNYYS
Sbjct: 363 LPFTTPP-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYS 422

Query: 363 HPDDLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCK 422
           +P DL+ CV G++K G+LL T  ++  K +DL                       E +C+
Sbjct: 423 NPTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCR 482

Query: 423 NTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
            +VA+YWHYHGGCLVGKV+D ++RV GI+ LRVVDGSTF  +P ++P    +MLG
Sbjct: 483 ESVASYWHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLG 533

[Close]

BLAST of Lsi11G007420 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.4e-109
Identity = 216/475 (45.47%), Postives = 293/475 (61.68%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           T+GCPLAATLS K+ +L+LERGS P  YP+VL   G +    +EDDGK P +RF+SEDG+
Sbjct: 63  TSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGI 122

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           +N+RGRVLGG S++NAG Y+R +   +  +GVDW+M+LV + YEWVE+T+V +P   ++W
Sbjct: 123 DNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKP-NSQSW 182

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            +  ++A LE GV P++GF L H  GT+I G    +K T       L +      ++   
Sbjct: 183 QSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVH 242

Query: 183 PQSRNSY--SLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPK 242
                    +   L+A GV Y DS G  H AF+R KGE+I+SAG IG+ QLLLLSGVGP+
Sbjct: 243 ASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPE 302

Query: 243 SHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGP 302
           S+LSSL +PVVL  P+VGQF+ DNPR   NI+ P  + P    V+GI  D  ++Q     
Sbjct: 303 SYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSS 362

Query: 303 SPFLVPPTFSLLPPHSTSI-NPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYS 362
            PF  PP F   P  S  + N + A F  K +   S GSL L SS +V+ SP V+FNYYS
Sbjct: 363 LPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYS 422

Query: 363 HPDDLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCK 422
           +  DL+ CV G++K G+LL T  ++  K +DL                       E +C+
Sbjct: 423 NLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCR 482

Query: 423 NTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
            +VA+YWHYHGGCLVGKV+D ++RV GI  LRVVDGSTF  +P ++P    +MLG
Sbjct: 483 ESVASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLG 533

[Close]

BLAST of Lsi11G007420 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 5.8e-109
Identity = 216/479 (45.09%), Postives = 292/479 (60.96%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLAATLS  F +LLLERG  P   P+V++  G +    + ++  +P Q FISE+GV
Sbjct: 63  TAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGV 122

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
            N RGRVLGG S +NAGFYSR  +QFFE +G+ W++  V ++YEWVE  +V RP  L+ W
Sbjct: 123 PNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRP-QLRTW 182

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPD------KETDMELWSFL----IRLILK 182
             A R ALLE GV P NGF L H VGTKIGG        + +  +L  +     IR+ + 
Sbjct: 183 QTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVY 242

Query: 183 TFKLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLS 242
               +    S  S S  ++SA GV Y D  G  H A IR +GE+ILSAGA+GS QLL LS
Sbjct: 243 ATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLS 302

Query: 243 GVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQ 302
           G+GP+S+LS+  +PV L QPHVG F+ DNPR G +IV P  +     +VVG+ ED  + +
Sbjct: 303 GIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLE 362

Query: 303 SLAGPSPFLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQF 362
           + +   PF  P     +   ++ +   + T   K     S G LRL +S DV+ +P+V+F
Sbjct: 363 AASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRF 422

Query: 363 NYYSHPDDLARCVRGVRKFGDLLKTPTMEKIKTQD-----------------------LE 422
           NY+S P DL RCV G RK G++L++  M+    ++                       + 
Sbjct: 423 NYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMA 482

Query: 423 EYCKNTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ++C+ TV+T WHYHGG +VGKVVD + +VIG+ +LR+VDGSTF+ISPGTNP ATLMMLG
Sbjct: 483 DFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLG 539

[Close]

BLAST of Lsi11G007420 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-107
Identity = 212/476 (44.54%), Postives = 291/476 (61.13%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLAATLS+ +S+L+LERGS P +YP++L   G +    +EDDGK P +RF+SEDG+
Sbjct: 63  TAGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGI 122

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           +N+RGRVLGG SM+NAG Y R +  FF + G++W+M+LV + YEWVE+T+V  P   + W
Sbjct: 123 DNVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEP-DSQTW 182

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGP------DKETDMELWSFLIRLILKTFKL 182
                +A LE G+ P+NGF + HL GT++ G        +    EL +      L+    
Sbjct: 183 QTVIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQ 242

Query: 183 QFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGP 242
               +   S +   ++A GV Y+DS G  H AF+R +GE+ILSAG IGS QLLLLSGVGP
Sbjct: 243 AAVEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGP 302

Query: 243 KSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAG 302
           +S+L+SL + VV   P+VGQ++ DNPR   NI+ P  +   +  V+GI  D  ++Q    
Sbjct: 303 ESYLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSIS 362

Query: 303 PSPFLVPPTFSLLPPHSTSI-NPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYY 362
             PF  PP FS  P  S  + N + A    K     S G++ LNSS DV+  P V+FNYY
Sbjct: 363 SLPFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYY 422

Query: 363 SHPDDLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYC 422
           S+  DL+ CV G++K G++L T  +E  K +DL                       E +C
Sbjct: 423 SNLTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFC 482

Query: 423 KNTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           + +VA+YWHYHGGCLVGKV+DD +RV GI  LRVVDGSTF  +P ++P    +MLG
Sbjct: 483 RESVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLG 534

[Close]

BLAST of Lsi11G007420 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 5.6e-104
Identity = 207/483 (42.86%), Postives = 290/483 (60.04%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           T+GCPLAATLS K+ +LLLERG+   +YP+ L   G      ++DDGK P +RF+SEDG+
Sbjct: 63  TSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGI 122

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           +N+R R+LGG +++NAG Y+R +  F+ + G++W+++LV K YEWVE+ +V +P   ++W
Sbjct: 123 DNVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKP-NNQSW 182

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGP------DKETDMELW------SFLIRLI 182
            +      LE G+ PDNGF L H  GT++ G        +    EL       + L+ + 
Sbjct: 183 QSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQ 242

Query: 183 LKTFKLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLL 242
               K+ F   + N      LSA GV Y+DS GN H AF+R  GE+I+SAG IG+ QLLL
Sbjct: 243 ASVEKILFSSNTSN------LSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLL 302

Query: 243 LSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILE-DNI 302
           LSGVGP+S+LSSL + VV   P+VGQF+ +NPR   N +  F   P+   VV +L   + 
Sbjct: 303 LSGVGPESYLSSLNITVVQPNPYVGQFLYNNPR---NFINNFPPNPIEASVVTVLGIRSD 362

Query: 303 YFQSLAGPSPFLVPPTFSLLPPHSTSI-NPSLATFFGKFSEVHSEGSLRLNSSIDVKKSP 362
           Y+Q      PF  PP FSL P  S  + N + A    +     S GS+ LNSS DV+ +P
Sbjct: 363 YYQVSLSSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAP 422

Query: 363 IVQFNYYSHPDDLARCVRGVRKFGDLLKTPTMEKIKTQDL-------------------- 422
            ++FNYYS+  DLA CV G++K GDLL+T  +E  K +D+                    
Sbjct: 423 NIKFNYYSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDD 482

Query: 423 ---EEYCKNTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLM 449
              E +C + VA+YWHYHGG LVGKV+DD++RV+GI+ LRVVD STF   P ++P    +
Sbjct: 483 ASFETFCLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYL 534

[Close]

BLAST of Lsi11G007420 vs. ExPASy TrEMBL
Match: A0A0A0LZU8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 3.3e-200
Identity = 359/472 (76.06%), Postives = 388/472 (82.20%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFS+LLLERGSDPNKYPSVLNEQGL+ AF  EDDGK PFQ F+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVNAGFYSR H++FFE AGVDW+MELVEKAYEWVEE+VVS+P ILK W
Sbjct: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQP-ILKDW 180

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            +AFRSALLEGG+ PDNGFDLRHLVGTK GG    DK         L +   K  K+  +
Sbjct: 181 QSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIE 240

Query: 183 PQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
              R       LSA GVSY DSKG LHTAFI +KGEI LSAGAIGS QLLLLSG+GPKSH
Sbjct: 241 ATVRR-ILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSH 300

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQPHVGQ M+DNPRFGTNI+LPFQ+VP SGKVVGIL+DNIYFQS+A PSP
Sbjct: 301 LSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSP 360

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPPTFSLLPPH TSINP+LA FFGKFSEVHS+GSL+LNSSIDVKKSPIVQFNYYSHPD
Sbjct: 361 FLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPD 420

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+GVRK GD+ KTPT+EKIKTQ+L                       EEYCK TV
Sbjct: 421 DLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTV 480

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ATYWHYHGGCLVGKVVD NY+VI  ENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 481 ATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of Lsi11G007420 vs. ExPASy TrEMBL
Match: A0A1S3BTS5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 3.2e-195
Identity = 353/472 (74.79%), Postives = 383/472 (81.14%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFSIL+LERGSDPNKYPSVLN QG+ NAF   DDGKNPFQ F+SEDGV
Sbjct: 60  TAGCPLATTLSSKFSILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGV 119

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVNAGFYSRGHR+FFE AGVDW+MELVEKAYEWVEETVVS+PI+  AW
Sbjct: 120 ENIRGRVLGGGSMVNAGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAW 179

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPDKETDMELWSFLIRLILKTFKLQFKPQS 182
            AAFRSALLEGGV PDNGFDLRHLVGTKIGG   +         + L+ K      K   
Sbjct: 180 QAAFRSALLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHG-AVELLNKANPTNIKVAI 239

Query: 183 RNSYSLV---SLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
           + +   +    LSA GVSYSDSKG LHTA I K GEII+SAGAIGS QLLLLSG+GPKSH
Sbjct: 240 QATVQKILFSGLSATGVSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSH 299

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQPHVGQFM+DNPRFGT IVLPFQL+P +GKVVGIL++NIY QS AGPS 
Sbjct: 300 LSSLKLPVVLHQPHVGQFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPST 359

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPP FSLLPP STSINP+LA+F GKFS+V+S+G L LNSS+DVKKSPIV+FNYYSH D
Sbjct: 360 FLVPPAFSLLPPQSTSINPTLASFVGKFSDVNSKGWLHLNSSLDVKKSPIVRFNYYSHHD 419

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDLE-----------------------EYCKNTV 422
           DL RCVRGVRK GDLLKT T+EKIKTQDLE                       EYCK TV
Sbjct: 420 DLDRCVRGVRKVGDLLKTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTV 479

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
            TYWHYHGGCLVGKVVD NY+VIGIENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 480 TTYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of Lsi11G007420 vs. ExPASy TrEMBL
Match: A0A5A7VHS6 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00760 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.1e-192
Identity = 351/472 (74.36%), Postives = 382/472 (80.93%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFS+LLLERGSDPNKYP VLNEQGL  AF  EDDGK PFQRF+SEDGV
Sbjct: 25  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 84

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVN GFYSRGH++FFE AGVDW+MELVEKAYEWVEETVVS+P ILK W
Sbjct: 85  ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQP-ILKDW 144

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            AAFRSALLEGG+ PDNGFDLRHLVGTK GG    +K         L +   K  K+  +
Sbjct: 145 QAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIE 204

Query: 183 PQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
              +       LSA+GVSY DS G LHTAFI KKGEI+LSAGAIGS QLLLLSG+GPKSH
Sbjct: 205 VTVQR-ILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSH 264

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQP+VG+ MSDNPRFG ++V+ FQ+VP + KVVGIL+DNIYFQSLAGPSP
Sbjct: 265 LSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSP 324

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPPTFSLLP   TSIN +LA F GKFSEV+S+GSL+LNSSIDVKKSPIVQFNYYSHP 
Sbjct: 325 FLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPG 384

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+ VRK GD+ KTPTMEKIKTQDL                       EEYCK TV
Sbjct: 385 DLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTV 444

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ATYWHYHGGCLVGKVVD NY+VIGIENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 445 ATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLG 494

[Close]

BLAST of Lsi11G007420 vs. ExPASy TrEMBL
Match: A0A1S3BSS8 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.1e-192
Identity = 351/472 (74.36%), Postives = 382/472 (80.93%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFS+LLLERGSDPNKYP VLNEQGL  AF  EDDGK PFQRF+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 120

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVN GFYSRGH++FFE AGVDW+MELVEKAYEWVEETVVS+P ILK W
Sbjct: 121 ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQP-ILKDW 180

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            AAFRSALLEGG+ PDNGFDLRHLVGTK GG    +K         L +   K  K+  +
Sbjct: 181 QAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIE 240

Query: 183 PQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
              +       LSA+GVSY DS G LHTAFI KKGEI+LSAGAIGS QLLLLSG+GPKSH
Sbjct: 241 VTVQR-ILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSH 300

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQP+VG+ MSDNPRFG ++V+ FQ+VP + KVVGIL+DNIYFQSLAGPSP
Sbjct: 301 LSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSP 360

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPPTFSLLP   TSIN +LA F GKFSEV+S+GSL+LNSSIDVKKSPIVQFNYYSHP 
Sbjct: 361 FLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPG 420

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+ VRK GD+ KTPTMEKIKTQDL                       EEYCK TV
Sbjct: 421 DLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTV 480

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ATYWHYHGGCLVGKVVD NY+VIGIENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 481 ATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of Lsi11G007420 vs. ExPASy TrEMBL
Match: A0A1S3BSR3 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.1e-192
Identity = 351/472 (74.36%), Postives = 382/472 (80.93%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFS+LLLERGSDPNKYP VLNEQGL  AF  EDDGK PFQRF+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 120

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVN GFYSRGH++FFE AGVDW+MELVEKAYEWVEETVVS+P ILK W
Sbjct: 121 ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQP-ILKDW 180

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            AAFRSALLEGG+ PDNGFDLRHLVGTK GG    +K         L +   K  K+  +
Sbjct: 181 QAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIE 240

Query: 183 PQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
              +       LSA+GVSY DS G LHTAFI KKGEI+LSAGAIGS QLLLLSG+GPKSH
Sbjct: 241 VTVQR-ILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSH 300

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQP+VG+ MSDNPRFG ++V+ FQ+VP + KVVGIL+DNIYFQSLAGPSP
Sbjct: 301 LSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSP 360

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPPTFSLLP   TSIN +LA F GKFSEV+S+GSL+LNSSIDVKKSPIVQFNYYSHP 
Sbjct: 361 FLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPG 420

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+ VRK GD+ KTPTMEKIKTQDL                       EEYCK TV
Sbjct: 421 DLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTV 480

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ATYWHYHGGCLVGKVVD NY+VIGIENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 481 ATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of Lsi11G007420 vs. NCBI nr
Match: XP_011650118.2 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical protein Csa_021306 [Cucumis sativus])

HSP 1 Score: 713.4 bits (1840), Expect = 1.2e-201
Identity = 362/472 (76.69%), Postives = 390/472 (82.63%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFS+LLLERGSDPNKYPSVLNE+GL  AF  EDDGK PFQRF+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEKGLSKAFDTEDDGKTPFQRFVSEDGV 120

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGR+LGGGSMVNAGFYSRGH++FFE AGVDW+MELVEKAYEW+EE+VVS+P ILK W
Sbjct: 121 ENIRGRILGGGSMVNAGFYSRGHKEFFESAGVDWDMELVEKAYEWIEESVVSQP-ILKDW 180

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            +AFRSALLEGG+ PDNGFDL HLVGTK GG    DK         L +   K  K+  +
Sbjct: 181 QSAFRSALLEGGIVPDNGFDLSHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIE 240

Query: 183 PQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
              +       LSA GVSY DSKG LHT FI +KGEII+SAGAIGS QLLLLSG+GPKSH
Sbjct: 241 ATVQR-ILFSDLSANGVSYLDSKGKLHTTFIHEKGEIIVSAGAIGSPQLLLLSGIGPKSH 300

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQPHVGQ MSDNPRFGTNIVLPFQ+VP SGKVVGIL+DNIYFQSLA PSP
Sbjct: 301 LSSLKLPVVLHQPHVGQSMSDNPRFGTNIVLPFQVVPTSGKVVGILQDNIYFQSLASPSP 360

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPPTFSLLPPH TSINP+LATFFGKFSEVHS+GSL+LNSSIDVKKSPIVQFNYYSHPD
Sbjct: 361 FLVPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPD 420

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+GVRK GD+ KTPTMEKIKTQDL                       EEYCK TV
Sbjct: 421 DLARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTV 480

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ATYWHYHGGCLVGKVVD NY+VIG ENLRVVDGSTFSISPGTNPMAT+MMLG
Sbjct: 481 ATYWHYHGGCLVGKVVDGNYKVIGTENLRVVDGSTFSISPGTNPMATVMMLG 530

[Close]

BLAST of Lsi11G007420 vs. NCBI nr
Match: XP_031736583.1 ((R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein Csa_021303 [Cucumis sativus])

HSP 1 Score: 704.9 bits (1818), Expect = 4.4e-199
Identity = 357/472 (75.64%), Postives = 388/472 (82.20%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFS+LLLERGSDPNKYPSVLNEQGL+ AF  EDDGK PFQ F+SEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVNAGFYSR H++FFE AGVDW+MELVEKAYEWVEE+VVS+P ILK W
Sbjct: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQP-ILKDW 180

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGG---PDKETDMELWSFLIRLILKTFKLQFK 182
            +AFRSALLEGG+ PDNGFDLRHLVGTK GG    DK         L +   K  K+  +
Sbjct: 181 QSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIE 240

Query: 183 PQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
              R       LSA GVSY DSKG LHTAFI +KGEI LSAGAIGS QLLLLSG+GPKSH
Sbjct: 241 ATVRR-ILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSH 300

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQPHVGQ M+DNPRFGTNI+LPFQ+VP SGKVVGIL+DNIYFQS+A PSP
Sbjct: 301 LSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSP 360

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPPTFSLLPPH TSINP+LA FFGKFSEVHS+GSL+LNSSIDVKK+PIV+FNYYSHPD
Sbjct: 361 FLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPD 420

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+GVRK GD+ KTPT+EKIKTQ+L                       EEYCK TV
Sbjct: 421 DLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTV 480

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ATYWHYHGGCLVGKVVD NY+VI  ENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 481 ATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of Lsi11G007420 vs. NCBI nr
Match: XP_008452065.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 691.0 bits (1782), Expect = 6.6e-195
Identity = 353/472 (74.79%), Postives = 383/472 (81.14%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLA TLSSKFSIL+LERGSDPNKYPSVLN QG+ NAF   DDGKNPFQ F+SEDGV
Sbjct: 60  TAGCPLATTLSSKFSILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGV 119

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVNAGFYSRGHR+FFE AGVDW+MELVEKAYEWVEETVVS+PI+  AW
Sbjct: 120 ENIRGRVLGGGSMVNAGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAW 179

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPDKETDMELWSFLIRLILKTFKLQFKPQS 182
            AAFRSALLEGGV PDNGFDLRHLVGTKIGG   +         + L+ K      K   
Sbjct: 180 QAAFRSALLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHG-AVELLNKANPTNIKVAI 239

Query: 183 RNSYSLV---SLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
           + +   +    LSA GVSYSDSKG LHTA I K GEII+SAGAIGS QLLLLSG+GPKSH
Sbjct: 240 QATVQKILFSGLSATGVSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSH 299

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQPHVGQFM+DNPRFGT IVLPFQL+P +GKVVGIL++NIY QS AGPS 
Sbjct: 300 LSSLKLPVVLHQPHVGQFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPST 359

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           FLVPP FSLLPP STSINP+LA+F GKFS+V+S+G L LNSS+DVKKSPIV+FNYYSH D
Sbjct: 360 FLVPPAFSLLPPQSTSINPTLASFVGKFSDVNSKGWLHLNSSLDVKKSPIVRFNYYSHHD 419

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDLE-----------------------EYCKNTV 422
           DL RCVRGVRK GDLLKT T+EKIKTQDLE                       EYCK TV
Sbjct: 420 DLDRCVRGVRKVGDLLKTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTV 479

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
            TYWHYHGGCLVGKVVD NY+VIGIENLRVVDGSTFS+SPGTNPMATLMMLG
Sbjct: 480 TTYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLG 530

[Close]

BLAST of Lsi11G007420 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 688.0 bits (1774), Expect = 5.6e-194
Identity = 356/472 (75.42%), Postives = 380/472 (80.51%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLAATLSSKFS+L+LERGSDPNKYPSVLNEQGL+N F   DDGKNPFQRFISEDGV
Sbjct: 60  TAGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGV 119

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIR RVLGGGSMVNAGFYSRGH+QFFE AGVDW+MELVEKAY+WVEETV SRP IL AW
Sbjct: 120 ENIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRP-ILNAW 179

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPDKETDMELWSFLIRLILKTFKLQFKPQS 182
            +AFRSALLE GV PDNGFDLRHLVGTK GG   + D       + L+ K      K   
Sbjct: 180 QSAFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFD-DKGNRHGAVELLNKAEPTNIKVAI 239

Query: 183 RNSYSLV---SLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
             +   +    LSARGV YSDSKG LH AFIRKKGEII+SAGAIGS QLLLLSGVGPKSH
Sbjct: 240 EATVQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSH 299

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQP+VG+FMSDNPRFGTNI+LPFQLVP S KVVGIL+DNIY QS A PSP
Sbjct: 300 LSSLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSP 359

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           F  PP+FSLLPP   SI PSLA F GKFSEVHSEGSLRLNSS +VKKSPIV+FNYYSHPD
Sbjct: 360 FFAPPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPD 419

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDLE-----------------------EYCKNTV 422
           DLARCV+GVRK GDLLKT TMEKIKT DLE                       EYC+ TV
Sbjct: 420 DLARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTV 479

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
            TYWHYHGGCLVGKVVD NY+VIGI+NLRVVDGSTFS SPGTNPMATLMMLG
Sbjct: 480 TTYWHYHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLG 529

[Close]

BLAST of Lsi11G007420 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 682.9 bits (1761), Expect = 1.8e-192
Identity = 351/472 (74.36%), Postives = 379/472 (80.30%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPL++TLSSKFS+LLLERGSDPNKYPSVLNEQGL+NAF  EDDGKNPFQ FISEDGV
Sbjct: 60  TAGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGV 119

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
           ENIRGRVLGGGSMVN GFYSRGH+QFFE AGVDW+MELVEKAY+WVEETVVS+P IL AW
Sbjct: 120 ENIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQP-ILNAW 179

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPDKETDMELWSFLIRLILKTFKLQFKPQS 182
            +AFRSA L+ GV PDNGFDLRH VGTKIGG   +         + L+ K      K   
Sbjct: 180 QSAFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHG-AVELLNKANPTNIKVGI 239

Query: 183 RNSYSLV---SLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLSGVGPKSH 242
             +   +    LSARGV YSDSKG LH AFIRK+GEII+SAGA+GS QLLLLSGVGPKSH
Sbjct: 240 EATVQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSH 299

Query: 243 LSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQSLAGPSP 302
           LSSLKLPVVLHQP+VG+FMSDNPRFGTNI+LPFQLVP S KVVGIL+DNIY QS A PSP
Sbjct: 300 LSSLKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSP 359

Query: 303 FLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPD 362
           F  PP+FSLLPP   SI PSLA F GKFSEVHSEGSLRLNSS +VKKSPIV+FNYYSHPD
Sbjct: 360 FFAPPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPD 419

Query: 363 DLARCVRGVRKFGDLLKTPTMEKIKTQDL-----------------------EEYCKNTV 422
           DLARCV+GVRK GDLLKT TMEKIKT DL                       EEYC+ TV
Sbjct: 420 DLARCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTV 479

Query: 423 ATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
            TYWHYHGGCLVGKVVD NY+VIGIENLRVVDGSTF  SPGTNPMATLMMLG
Sbjct: 480 TTYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFCDSPGTNPMATLMMLG 529

[Close]

BLAST of Lsi11G007420 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 4.1e-110
Identity = 216/479 (45.09%), Postives = 292/479 (60.96%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLAATLS  F +LLLERG  P   P+V++  G +    + ++  +P Q FISE+GV
Sbjct: 63  TAGCPLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGV 122

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
            N RGRVLGG S +NAGFYSR  +QFFE +G+ W++  V ++YEWVE  +V RP  L+ W
Sbjct: 123 PNARGRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRP-QLRTW 182

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGPD------KETDMELWSFL----IRLILK 182
             A R ALLE GV P NGF L H VGTKIGG        + +  +L  +     IR+ + 
Sbjct: 183 QTAIRDALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVY 242

Query: 183 TFKLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIGSSQLLLLS 242
               +    S  S S  ++SA GV Y D  G  H A IR +GE+ILSAGA+GS QLL LS
Sbjct: 243 ATVERVLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLS 302

Query: 243 GVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIYFQ 302
           G+GP+S+LS+  +PV L QPHVG F+ DNPR G +IV P  +     +VVG+ ED  + +
Sbjct: 303 GIGPRSYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLE 362

Query: 303 SLAGPSPFLVPPTFSLLPPHSTSINPSLATFFGKFSEVHSEGSLRLNSSIDVKKSPIVQF 362
           + +   PF  P     +   ++ +   + T   K     S G LRL +S DV+ +P+V+F
Sbjct: 363 AASNVIPFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRF 422

Query: 363 NYYSHPDDLARCVRGVRKFGDLLKTPTMEKIKTQD-----------------------LE 422
           NY+S P DL RCV G RK G++L++  M+    ++                       + 
Sbjct: 423 NYFSDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMA 482

Query: 423 EYCKNTVATYWHYHGGCLVGKVVDDNYRVIGIENLRVVDGSTFSISPGTNPMATLMMLG 449
           ++C+ TV+T WHYHGG +VGKVVD + +VIG+ +LR+VDGSTF+ISPGTNP ATLMMLG
Sbjct: 483 DFCRRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLG 539

[Close]

BLAST of Lsi11G007420 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 308.5 bits (789), Expect = 8.6e-84
Identity = 193/511 (37.77%), Postives = 267/511 (52.25%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGCPLAATLS   S+LLLERG  P   P++        A + +    +P QRF+SEDGV
Sbjct: 55  TAGCPLAATLSQNASVLLLERGDSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGV 114

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
            N R RVLGGGS +NAGFY+R   ++    G  W+  L  ++Y+WVE  V  +P  +  W
Sbjct: 115 INARARVLGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQP-PMGRW 174

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGP------DKETDMELWSFL----IRLILK 182
             A R  LLE G+ P+NGF   H+ GTK GG       ++ T  +L  +     I ++L 
Sbjct: 175 QTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLH 234

Query: 183 TFKLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRK--KGEIILSAGAIGSSQLLL 242
               +   ++R +   +   A GV Y D  G  H A++++    EIILSAG +GS QLL+
Sbjct: 235 ATVHRILFRTRGTTKPI---ANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLM 294

Query: 243 LSGVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVGILEDNIY 302
           LSGVGP + L +  + VV+ QPHVGQ M DNP     +  P  +     +VVGI  +  Y
Sbjct: 295 LSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTY 354

Query: 303 FQSLAGPS-----------------PFLVPPTFSLLPPHSTSINPSLATFFG-----KFS 362
            ++  G +                   +  P  +LL  +S +   S   F G     K  
Sbjct: 355 VEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVM 414

Query: 363 EVHSEGSLRLNSSIDVKKSPIVQFNYYSHPDDLARCVRGVRKFGDLLKTPTMEKIKTQDL 422
              S G L L +  + K +PIV FNY+ HPDDL RCVRG++    ++++    + K  D+
Sbjct: 415 GPLSTGHLELKTR-NPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV 474

Query: 423 -------------------------------EEYCKNTVATYWHYHGGCLVGKVVDDNYR 449
                                          EE+C++TV T WHYHGGC+VG+VVD +Y+
Sbjct: 475 SFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYK 534

[Close]

BLAST of Lsi11G007420 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 300.4 bits (768), Expect = 2.3e-81
Identity = 203/507 (40.04%), Postives = 277/507 (54.64%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGC LAATLS   S+L+LERG  P + P+  +    +N  +  +   +  Q FISEDGV
Sbjct: 82  TAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSVNTLL-NNTPNSWSQLFISEDGV 141

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
            N R RVLGGGS++N GFYSR    + E A  +WEME VE AYEWVE+ +V  P +++ W
Sbjct: 142 YNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIE-W 201

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGP--DKETDMELWSFLIRLILKTFKLQFKP 182
             AF+  LLE G +PDNGF   H+ GTKIGG   D+       + L+        + +  
Sbjct: 202 QKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLH 261

Query: 183 QSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKK--GEIILSAGAIGSSQLLLLSGVGPKS 242
            S +   L +  A  V + D+ G  H A +  K   E+ILSAGA+GS QLL+LSGVGP  
Sbjct: 262 ASVHKV-LFTTEAYEVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAV 321

Query: 243 HLSSLKL-PVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLS-GKVVGILEDNIYFQSLAG 302
           HL +  + P+VL QP VGQ M+DNP     I  P Q V LS  + VGI + + Y + L+G
Sbjct: 322 HLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSP-QPVELSLIQAVGITKFDSYIEGLSG 381

Query: 303 PS-PFLVPPTF-----SLL--PPHSTS---INPSLAT-----------------FFGKFS 362
            S  F +   F     +LL    H+TS   +  S+A                   F K  
Sbjct: 382 LSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVD 441

Query: 363 EVHSEGSLRLNSSIDVKKSPIVQFNYYSHPDDLARCVRGVRKFGDLLKTPTMEKIK---- 422
              S+G ++L ++ + + +P V FNYY  P+DL +CV+G+     ++ +    K K    
Sbjct: 442 GPASKGHMKLRNT-NPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGV 501

Query: 423 -----------------------TQDLEEYCKNTVATYWHYHGGCLVGKVVDDNYRVIGI 449
                                    +L+++C +TV + WHYHGGC VGKVVD NY+V+GI
Sbjct: 502 TARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGI 561

[Close]

BLAST of Lsi11G007420 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 298.5 bits (763), Expect = 8.9e-81
Identity = 199/516 (38.57%), Postives = 267/516 (51.74%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDP--NKYPSVLNEQGLMNAFVEEDDGKNPFQRFISED 62
           TAGCPLAATLS  FS+L+LERG  P  N   S L      +  + +    +  Q F+S D
Sbjct: 72  TAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADISASSASQAFVSTD 131

Query: 63  GVENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILK 122
           GV N R RVLGGGS +NAGFYSR    F +RAG  W+ +LV+++Y WVE  +V +P  L 
Sbjct: 132 GVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVEREIVHQP-KLT 191

Query: 123 AWPAAFRSALLEGGVAPDNGFDLRHLVGTKIGGP------DKETDMELWSFLIRLILKTF 182
            W  A R +LLE GV P NGF   H+ GTKIGG        + T  EL ++     L+  
Sbjct: 192 LWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYANPQKLRVL 251

Query: 183 KLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFI--RKKGEIILSAGAIGSSQLLLLS 242
                 +     S       GV + D KGN H A +  RK  E+ILS+GAIGS Q+L+LS
Sbjct: 252 IYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAIGSPQMLMLS 311

Query: 243 GVGPKSHLSSLKLPVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSG---KVVGILEDNI 302
           G+GPK  L  LK+PVVL   HVG+ M+DNP    N +L     P+     + VGI +  +
Sbjct: 312 GIGPKKELQRLKIPVVLENEHVGKGMADNP---MNTILVPSKAPIEQSLIQTVGITKMGV 371

Query: 303 YFQSLA--GPSPFLV----------PPTFSLLPPHSTSINPSLATFFGKFSEVH------ 362
           Y ++    G SP  +             FS +P        + A       ++H      
Sbjct: 372 YVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQLHEAFNGS 431

Query: 363 ----------SEGSLRL-NSSIDVKKSPIVQFNYYSHPDDLARCVRGVRKFGDLLKT--- 422
                     S G L L N+++D   +P V FNY+ HP DL RCV  +R    ++ +   
Sbjct: 432 FILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKHPVDLQRCVEAIRLVSKVVTSNRF 491

Query: 423 --------PTMEKI-----------------KTQDLEEYCKNTVATYWHYHGGCLVGKVV 449
                     + K+                  T+ + ++CK+TV T WHYHGGCLVGKVV
Sbjct: 492 LNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHGGCLVGKVV 551

[Close]

BLAST of Lsi11G007420 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.3e-79
Identity = 202/514 (39.30%), Postives = 263/514 (51.17%), Query Frame = 0


Query: 3   TAGCPLAATLSSKFSILLLERGSDPNKYPSVLNEQGLMNAFVEEDDGKNPFQRFISEDGV 62
           TAGC LAATLS   ++L+LERG  P   P+  +     N  +      +  Q FISEDGV
Sbjct: 55  TAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNFANTLLNITP-NSWSQLFISEDGV 114

Query: 63  ENIRGRVLGGGSMVNAGFYSRGHRQFFERAGVDWEMELVEKAYEWVEETVVSRPIILKAW 122
            N R RVLGGG+++NAGFYSR    F   AG  WE + VE AYEWVE+ VV  P + K W
Sbjct: 115 FNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNK-W 174

Query: 123 PAAFRSALLEGGVAPDNGFDLRHLVGTKIGGP------DKETDMELWSFL---------- 182
            +AFR  LLE GV P NGF   H+VGTK GG        + T   L  +           
Sbjct: 175 QSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLH 234

Query: 183 --IRLILKTFKLQFKPQSRNSYSLVSLSARGVSYSDSKGNLHTAFIRKKGEIILSAGAIG 242
             +  IL T K   +P+   +Y ++ L A GVSY   K  L T       E+ILSAGAI 
Sbjct: 235 ASVHKILFTIKGNQRPK---AYGVIFLDANGVSY---KAELATQ-DSTMSEVILSAGAIA 294

Query: 243 SSQLLLLSGVGPKSHLSSLKL-PVVLHQPHVGQFMSDNPRFGTNIVLPFQLVPLSGKVVG 302
           S QLL+LSGVGP +HL++ ++ PV++ QP VGQ M DNP     I  P  +     + VG
Sbjct: 295 SPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVG 354

Query: 303 ILEDNIYFQ--SLAGPSPFLVPPTF---------SLLPPHSTS-----------INPSLA 362
           I +   Y +  S    S  L    F         + LP  S S           +     
Sbjct: 355 ITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAG 414

Query: 363 TFFGKFSEVHSEGSLRLNSSIDVKKSPIVQFNYYSHPDDLARCVRGVRKFGDLLKTPTME 422
               K +   S G L L ++ +   +P V FNY+  P+DL +CV G+     ++ +    
Sbjct: 415 VIIQKVNGPLSRGHLELRNT-NPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYS 474

Query: 423 KIK---------------------------TQDLEEYCKNTVATYWHYHGGCLVGKVVDD 449
           K K                           T DLE+YC +TV T +HYHGGC VGKVVD+
Sbjct: 475 KYKYPLASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDN 534

The following BLAST results are available for this feature:

BLAST of Lsi11G007420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52706	1.2e-111	46.32	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	3.4e-109	45.47	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
Q9SSM2	5.8e-109	45.09	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
P52707	1.9e-107	44.54	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
O24243	5.6e-104	42.86	(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1	[more]

BLAST of Lsi11G007420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LZU8	3.3e-200	76.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1	[more]
A0A1S3BTS5	3.2e-195	74.79	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=...	[more]
A0A5A7VHS6	1.1e-192	74.36	(R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BSS8	1.1e-192	74.36	(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034931...	[more]
A0A1S3BSR3	1.1e-192	74.36	(R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034931...	[more]

BLAST of Lsi11G007420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650118.2	1.2e-201	76.69	(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical prote...	[more]
XP_031736583.1	4.4e-199	75.64	(R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein ...	[more]
XP_008452065.1	6.6e-195	74.79	PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo]	[more]
XP_038889314.1	5.6e-194	75.42	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]
XP_038889315.1	1.8e-192	74.36	(R)-mandelonitrile lyase 1-like [Benincasa hispida]	[more]

BLAST of Lsi11G007420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	4.1e-110	45.09	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	8.6e-84	37.77	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	2.3e-81	40.04	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	8.9e-81	38.57	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT3G56060.1	1.3e-79	39.30	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 3..448 e-value: 8.5E-131 score: 439.2
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 4..448
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 4..109 e-value: 2.0E-5 score: 24.1 coord: 193..261 e-value: 5.1E-13 score: 49.1
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 221..235
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 67..90
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 301..448 e-value: 2.3E-27 score: 93.2 coord: 3..131 e-value: 5.3E-17 score: 59.0 coord: 171..288 e-value: 6.8E-20 score: 68.5
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 331..448 e-value: 9.6E-26 score: 91.0
None	No IPR available	GENE3D	3.30.410.40		coord: 114..398 e-value: 8.5E-131 score: 439.2
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 3..389 coord: 387..448
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 3..389 coord: 387..448
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 313..405

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G007420.1	Lsi11G007420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors