Lsi11G007410 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G007410
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description(R)-mandelonitrile lyase 1-like
Locationchr11: 8116773 .. 8125557 (-)
RNA-Seq ExpressionLsi11G007410
SyntenyLsi11G007410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAGTTAGCTAATCTACACGTCATTATTTGAATAATTTCTTATATATAATTCATCATTATATTCAAATCATCATCCTAAACCTAATTGAAATTGAAAATTCAACCAATATGAGCATTAGTTTTCAACTTTTCTAGAGTCTCTTTAATTTAATCCTCCCTTATATTATTCTATAAATATTGACTTCATTGTCTTTTTAATATAGTCAATTTTGTATATGCTTATTCATTTCCTACTAGGAATAAATTGAGCCCATTCACCAATCGGATTAGATTAGAGCAACATTTATAACAACATATATATTACTTTTATTTGAAAATAATTAGGGTGGCGGATCAAACTCTTGATTCTCTTAAACTCGAAGCCTAATGGAGGACAAATAGAAATCTTATCGTGGAAGTCATTAAGAAGAAATATCTCCATTACATTGAGTAACAAGCTTGGATCATTGGCTAATACAAGCTAATCTTCATTTTCTTTAATTACATTTTAATTAAGCTTTTAAATATAAGATTATTTTATAGCATTATTATCAACAGATTAGAAGTGTACGTGAATATATATTGCAAGGGCATAATATTATTTTTTTTAGATAAGAGTCAAATATACATTTCATTGTATTTCTAAAAAAATAAATACATAACTATCACATTTGGGTTTTTATATATTATTACACATCATTATCATTTTTGTCATCATCGCAAGGAATATAAAATACATCCAGTATATAAAGCCTGTATCAGGAAGAAAATAATTTCTTTATTTAATAATAATTATTACAATATGCTATTCAATTCTGTCTTCATTACAAATTAAGATAACCTAGCCCAACCCTTTGTTCTCTTTTAATTATACTAGTTTTTTTTTTTTTAAAGTACGATTGGGATAGGAAATTCCAACCACAAACTTCGTGGTCACTAATACACTCGTATACCGGTTGAGTTAGGCTCGCTTTAGCTTAATTATACTAGTTTGTTACTAATAGGAAAAATTAAGGTTATATTATAAGGTTAGTCTATGAAGATTCAACTTTTATGTCATTATATTTTGATTCTTGAAAGTTTAAGAAGTGTTTAATAAGTTATTAAACTTTTGATTTTGTGTTTTTTTAACAAGATACTAGTGTAAGGATCAGTAGGTGTACCGGGACATCTCAACTAGGTTGACACCCCCTCTAGCACCCTCATCATGCCCCAGAGCCAGTGTATAACATTAAGCCAAAAAGAGGCGATCGGAGAGATCAAATGTTAAGTACAAATCGAGCTAGAGTGAAGCTCCGATCCAAAAAAAGAGTGAGGAAAGAGTAAAAGGAAAAACTAAAGAAAAGCCGTCCAGTTTAAAGCAATAGTAGGAGCGTCATCATTACAAAATAAAGGTGACTTTGTGCTACAAAGGGCGGCCAATGCGTTAATGTCCTCCCACCGTTGATGTTGTGATATTGTGATTTGTTATTTAGATCTTTCAACTTTTTAAAATATCTAATAAAGTCCTAAAAAATTTCAATTTTATATCCCATATGTCTCTTAATTATTCATTGGTTTACTAAATCTATGATGTATTCAACATTTATTTTTAAATTGATGGATGCACTAAATAAAAATTGAAATATTAAATTTACATTAAACATAAAATTCAACTGAATATCTATTACATTAGTTAATTTTAAATTTTTTTTAATATGTGATAGAACTATTGAATAGAAAATTGAAAGTTGAGGAATTATTAGACAATTTTAAAACCTAGGGATATGTGAGACATAAAATTCAAAGTTGAAAATCTATACTTAATTGTTCAAAAACAAAATAGATGTAGCAACCATTTAGAATCAATTCTTAAGATGTTCTCACCTCTTTGCTTTCTAAATAACATAGATAATTAATACAAAAATATAAAAATTAGAACAAATAGTACTAAACATATTTACTCATCTCTATTTATCATCCAATATTATTAATACCAAAAGAAATATAAGGAAAAATAATAAATTTTTATTTCTTTTATAATATGTGGGGTTGAAATTTTTTTTTTTTTGGAACAAAATACTAGAGACAAGTGGGACAGGAGATACACAAGGATATCTCAACTAGGTTGACACATTCGTAGCACTCTCATCATGCCCTGAGATCAAAAGTGATGCATTCATTCAAAAAATAGTATTCGTACAGGATGTGGAATCGGAGAATAGAACTTTACACACGAGATTGATTAATAATAAAAGTTTTATACCAACTAAATTATGCTTAATTTGACTATATATATATATATATATATATAATTGGGTGCTCAACAACTTATTTTTTATTCTTGATCATAGCATCATAATCTTATTCGAAAGTGAATGTCACAACACGCACCGAATCCCATCATAATTCTGCAGTTAAGCGTGCTTGAACAAGAATAATACTGTAAACACGTCAATTGTGGCACTTTGGACAATATAGATGGAAAGGAATAATCGCACCTTCAAAGACATGTTCAGAAGCAATGTCAATGTTTGGGAAGATATTTGCATCTTCTGGCGAAGCTGGTCACACAAGTTGAAACTACTCAAAGACTACAACCCCACTTCTATTGCCATTAACTTAGCTGCTTTATGTATGCTTAACGCTAGCTGCCTTTGTTGTTTTTTCCTTCATCAGGCTTTCTTCTAGCCCCTCTGTTTTTTTTAACTGCTTTTTGTATGATCTTTCGGGCTTTATTGAATATTTATAAAAGATCGTTATGATGAAGGTGCTAAGAATATGTCAACTTAAATAAGATATTTGAGTGCATCTACTAACCTATATCTTATCTTCTTCTAAAACAAAATTAAAGAAAGTCAATGTTACAGCAACTTTTATGAAATTAAAGTATTCATGAATTAATATTTTAGCTTTAAACACTGCTTATACTCATATATAATAATTATTATCTATTTTTTGTTCAAGATGTCGTATTTCTTCCACCCCAATTGCAAGAAATCTTCATGATATATTTCAGATCAAAGATCTCAATCCTCTAAAAAATTGTTGTTAGAAATCATAGTAATTCAAAAAAGAATTAGTAGAAAGTCATATACCATATCTACAAAATATCATTGAACTCAAGAAGAAAGAGTTTAACTTTATTTTGGATGATGAAAAATCTGTGTAATAATTTCATTCCTTTTCTTTTGTTTTTTTAGAAAATTAAATCATTTCTTAAACAAAATATAAAATTTGATTTAAAAATTTGGTAGCACACAAGAAAAGACTTGTTAAATTTGTTTTTAAAAAATGATAGTTAAATTTGACCAACCATTTACATGCATTCAAATTAAGAGTCAAACGTAACTCTGTATGTATATAAATATATATACCAACAGTTTCAAGCTGGAGAAGGAACAAAAACTTGTATCCTTACATTTAGTTTTTAGAAACTATGGAGCATTCTAGGGGTACCATTCTCATACTCAGCCTTATAATCTCTATATTTCAATTAGCAGTTCTCTCCTCACATGCAATTCCCAATCAAGGTAAAATTTTGCCTCTCAAATTACAAACTTTTCTCTTAATTAATGAATTCTTATTGATCTAAAATTCCTTTTTTCAGATTTTAGCTACATGAAGTTTGTACATAATGCCAGCGATTTGCCAGCAAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGTTGTCCATTAGCTACAACATTATCATCAAATTTCTCAGTCCTCCTTCTTGAAAGAGGCAGTGATCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAATGTTTTTGCTGCTGGAGATGATGGTAAAAATCCCTTCCAACGCTTCACCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGAGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGCCATCGACAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGACATCTCGACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGCTGGTGTTTTACCTGATAATGGGTTTGATTTGAGGCATCTTGTCGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTTGAGCTTCTCAATAAGGCTAAACCCACCAATATTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTTTCTGGTTAGTCCTAGTAAATATTCTATTTTAATTATATTTTTTGTATTTATTTTTTTGAGTTTTTTTTTTTTTTTTTTTTTTTTTCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN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mRNA sequence

ATGGAAACTATGGAGCATTCTAGGGGTACCATTCTCATACTCAGCCTTATAATCTCTATATTTCAATTAGCAGTTCTCTCCTCACATGCAATTCCCAATCAAGATTTTAGCTACATGAAGTTTGTACATAATGCCAGCGATTTGCCAGCAAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGTTGTCCATTAGCTACAACATTATCATCAAATTTCTCAGTCCTCCTTCTTGAAAGAGGCAGTGATCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAATGTTTTTGCTGCTGGAGATGATGGTAAAAATCCCTTCCAACGCTTCACCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGAGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGCCATCGACAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGACATCTCGACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGCTGGTGTTTTACCTGATAATGGGTTTGATTTGAGGCATCTTGTCGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTTGAGCTTCTCAATAAGGCTAAACCCACCAATATTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTTTCTGGTTTAACTGCAAGTGGGGTTTTGTATTCTGATTCAAAAGGAAAACTACATAGAGCATTCATCCGTGAGAAAGGTGAAATAATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCTTTAAAATTACCTGTCGTTCTTCACCAACCACATATCGGTGAATTCATGTCGGACAATCCCCGTTTTGGGACAAACATCATCCTTCCATTCAAATTGCTTCCTTCCTCTGTAAAAGTTGTTGGAATTTTAGAAGACAATATCTATATACAATCTTTTGCCAGCCCTTCACCAATTTTAGTTCCCCCAATTTTCAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCCAGCTTAGCCATGTTTGTTGGAAAATTCTCGGAGGTGCATTCTGAAGGCTCACTCCGATTGAATTCCTCCACTAATGTGAAGAAGAGCCCCATTGTTCGATTCAATTACTATTCACATCCTGATGATCTTGCTCGATGTGTTAGTGGAGTAAGAAAAATGGGAGATTTGCTTAAAACCCAAACCATGGAAAAGATTAAGACCCAAGATTTGGAGGGTAATAAAGTATTTGAGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTTGAATGATAGCTCCGTAGAAGAGTATTGTCATAAGACAGTGACAACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAAGTCATCGGAATAAAAAATTTGCGTATATGTTGGCCTGAAGGTACTGCAACAAAGATCGAGTGTCAACTAAGAGAGGCCATTATTGCTTAATATTCTTGGTTGTCGGGTGCATGTATCGTGTCTTCTTTAATTTTTGAACTATGTGTTTTATTATAATTGTTATTTATTTTTCGTTTTTTATATTTTCAGTTTCTAAAGATTGTATACAAATTTTATAAAACAAAAAGATGGGTTTTCTTATTCCCATTTTCTAGTTCGTCCAACAACATGAATAAATTAAGTCCAAGTTTGGAAGTAATTCTACCATTGTTATGAGTGTATTGTTGAATGAAAAATTTTGGACCAATCAC

Coding sequence (CDS)

ATGGAAACTATGGAGCATTCTAGGGGTACCATTCTCATACTCAGCCTTATAATCTCTATATTTCAATTAGCAGTTCTCTCCTCACATGCAATTCCCAATCAAGATTTTAGCTACATGAAGTTTGTACATAATGCCAGCGATTTGCCAGCAAAAGAAGAATATGATTACATAATAATAGGAGGAGGAACAGCAGGTTGTCCATTAGCTACAACATTATCATCAAATTTCTCAGTCCTCCTTCTTGAAAGAGGCAGTGATCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAATGTTTTTGCTGCTGGAGATGATGGTAAAAATCCCTTCCAACGCTTCACCTCTGAGGATGGTGTAGAGAATATAAGAGGGCGAGTCCTTGGCGGTGGAAGTATGGTTAATGCTGGCTTTTACTCGAGAGGCCATCGACAGTTCTTTGAAACAGCAGGTGTTGATTGGGATATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGACTGTGACATCTCGACCAATTTTGAATGCTTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGCTGGTGTTTTACCTGATAATGGGTTTGATTTGAGGCATCTTGTCGGAACTAAAACTGGAGGTTCCATCTTTGATGACAAAGGAAACAGACATGGAGCTGTTGAGCTTCTCAATAAGGCTAAACCCACCAATATTAAAGTTGCAATTGAAGCCACAGTCCAAAGAATCCTCTTTTCTGGTTTAACTGCAAGTGGGGTTTTGTATTCTGATTCAAAAGGAAAACTACATAGAGCATTCATCCGTGAGAAAGGTGAAATAATTGTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTTATCTTTCATCTTTAAAATTACCTGTCGTTCTTCACCAACCACATATCGGTGAATTCATGTCGGACAATCCCCGTTTTGGGACAAACATCATCCTTCCATTCAAATTGCTTCCTTCCTCTGTAAAAGTTGTTGGAATTTTAGAAGACAATATCTATATACAATCTTTTGCCAGCCCTTCACCAATTTTAGTTCCCCCAATTTTCAGTCTTCTTCCTCCTCAATCCACTTCCATCATCCCCAGCTTAGCCATGTTTGTTGGAAAATTCTCGGAGGTGCATTCTGAAGGCTCACTCCGATTGAATTCCTCCACTAATGTGAAGAAGAGCCCCATTGTTCGATTCAATTACTATTCACATCCTGATGATCTTGCTCGATGTGTTAGTGGAGTAAGAAAAATGGGAGATTTGCTTAAAACCCAAACCATGGAAAAGATTAAGACCCAAGATTTGGAGGGTAATAAAGTATTTGAGTTTTTGGGGCTTCCCTTGCCGGAAAATTTGTTGAATGATAGCTCCGTAGAAGAGTATTGTCATAAGACAGTGACAACTTATTGGCATTACCATGGAGGATGTTTGGTCGGAAAAGTGGTCGACGGTAATTATAAAGTCATCGGAATAAAAAATTTGCGTATATGTTGGCCTGAAGGTACTGCAACAAAGATCGAGTGTCAACTAAGAGAGGCCATTATTGCTTAA

Protein sequence

METMEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRICWPEGTATKIECQLREAIIA
Homology
BLAST of Lsi11G007410 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.3e-147
Identity = 261/505 (51.68%), Postives = 351/505 (69.50%), Query Frame = 0

Query: 11  ILILSLIISIFQLAVLSSHA-IPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLA 70
           +L+L L + + Q + + S A   N DFSY++F ++A+DL  +  YDY+I+GGGT+GCPLA
Sbjct: 10  LLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLA 69

Query: 71  TTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRV 130
            TLS  + VL+LERGS P  YP+VL   G +      DDGK P +RF SEDG++N+RGRV
Sbjct: 70  ATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRV 129

Query: 131 LGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSAL 190
           LGG SM+NAG Y+R +   +  +GVDWDM+LV K YEWVE+T+  +P    WQS   +A 
Sbjct: 130 LGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAF 189

Query: 191 LEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF 250
           LEAGV P++GF L H  GT+  GS FD+KG RH A ELLNK    N++V + A+V++I+F
Sbjct: 190 LEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIF 249

Query: 251 S---GLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKL 310
           S   GLTA+GV+Y DS G  HRAF+R KGE+IVSAG IG+PQLLLLSGVGP+SYLSSL +
Sbjct: 250 SNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNI 309

Query: 311 PVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPPI 370
           PVVL  P++G+F+ DNPR   NI+ P  + P+ V V+GI  D  + Q   S  P   PP 
Sbjct: 310 PVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTPP- 369

Query: 371 FSLLPPQSTSIIPS-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARC 430
           FS  P  S  +  S  A F  K +   S GSL L SS+NV+ SP V+FNYYS+P DL+ C
Sbjct: 370 FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHC 429

Query: 431 VSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYWH 490
           VSG++K+G+LL T  ++  K +DL G + F  LG+PLP++  +D++ E +C ++V +YWH
Sbjct: 430 VSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWH 489

Query: 491 YHGGCLVGKVVDGNYKVIGIKNLRI 511
           YHGGCLVGKV+DG+++V GI  LR+
Sbjct: 490 YHGGCLVGKVLDGDFRVTGIDALRV 511

BLAST of Lsi11G007410 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 3.5e-145
Identity = 257/514 (50.00%), Postives = 352/514 (68.48%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAI---PNQDFSYMKFVHNASDLPAKEEYDYIIIG 63
           ME S  + ++L L I +  L     H++    + DFSY+ F ++A+DL  +  YDY+I+G
Sbjct: 1   MEKSTMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVG 60

Query: 64  GGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSED 123
           GGT+GCPLA TLS  + VL+LERGS P  YP+VL   G +      DDGK P +RF SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSED 120

Query: 124 GVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNA 183
           G++N+RGRVLGG S++NAG Y+R +   +  +GVDWDM+LV + YEWVE+T+  +P   +
Sbjct: 121 GIDNVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQS 180

Query: 184 WQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAI 243
           WQS  ++A LEAGV P++GF L H  GT+  GS FD+KG RH A ELLNK    N++V +
Sbjct: 181 WQSVTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGV 240

Query: 244 EATVQRILFS---GLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGP 303
            A+V++I+FS   GLTA+GV+Y DS G  H+AF+R KGE+IVSAG IG+PQLLLLSGVGP
Sbjct: 241 HASVEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGP 300

Query: 304 KSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFAS 363
           +SYLSSL +PVVL  P++G+F+ DNPR   NI+ P  + P+ V V+GI  D  + Q   S
Sbjct: 301 ESYLSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFS 360

Query: 364 PSPILVPPIFSLLPPQSTSIIPS-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYY 423
             P   PP F   P  S  +  S  A F  K +   S GSL L SS+NV+ SP V+FNYY
Sbjct: 361 SLPFTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYY 420

Query: 424 SHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYC 483
           S+  DL+ CVSG++K+G+LL T  ++  K +DL G + F  LG+PLP++  +D++ E +C
Sbjct: 421 SNLTDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFC 480

Query: 484 HKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRI 511
            ++V +YWHYHGGCLVGKV+DG+++V GI  LR+
Sbjct: 481 RESVASYWHYHGGCLVGKVLDGDFRVTGINALRV 511

BLAST of Lsi11G007410 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.4e-141
Identity = 250/506 (49.41%), Postives = 347/506 (68.58%), Query Frame = 0

Query: 11  ILILSLIISIFQLAVLSSHA-IPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLA 70
           +L+L + +   Q + + S A   + DFSY+ FV++A+D   +  YDYII+GGGTAGCPLA
Sbjct: 10  LLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLA 69

Query: 71  TTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRV 130
            TLS+N+SVL+LERGS P +YP++L   G +      DDGK P +RF SEDG++N+RGRV
Sbjct: 70  ATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRV 129

Query: 131 LGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSAL 190
           LGG SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+   P    WQ+   +A 
Sbjct: 130 LGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAY 189

Query: 191 LEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF 250
           LEAG+LP+NGF + HL GT+  GS FD+ G RH + ELLNK  P N++VA++A V++I+F
Sbjct: 190 LEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIF 249

Query: 251 ----SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLK 310
               SG+TA GV+Y+DS G  H+AF+R +GE+I+SAG IGSPQLLLLSGVGP+SYL+SL 
Sbjct: 250 SSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLN 309

Query: 311 LPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPP 370
           + VV   P++G+++ DNPR   NI+ P  +  S+V V+GI  D  + Q   S  P   PP
Sbjct: 310 ISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPP 369

Query: 371 IFSLLPPQSTSI-IPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLAR 430
            FS  P  S  +   + A  V K     S G++ LNSS++V+  P V+FNYYS+  DL+ 
Sbjct: 370 -FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSH 429

Query: 431 CVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYW 490
           CVSG++K+G++L T  +E  K +DL G   F  LG+PLPEN  +D++ E +C ++V +YW
Sbjct: 430 CVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYW 489

Query: 491 HYHGGCLVGKVVDGNYKVIGIKNLRI 511
           HYHGGCLVGKV+D  ++V GI  LR+
Sbjct: 490 HYHGGCLVGKVLDDGFRVTGINALRV 512

BLAST of Lsi11G007410 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 4.0e-141
Identity = 255/516 (49.42%), Postives = 343/516 (66.47%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPN----QDFSYMKFVHNASDLPAKEEYDYIII 63
           ME S  + ++L L + +  L     H++ N     DF Y+KFV+NA DL  +  YDYII+
Sbjct: 1   MEKSTMSAVVLVLNLLVLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIV 60

Query: 64  GGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSE 123
           GGGT+GCPLA TLS+N+SVL+LERG+   +YP+ L   G        DDGK P +RF SE
Sbjct: 61  GGGTSGCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSE 120

Query: 124 DGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILN 183
           DG++N+R R+LGG +++NAG Y+R +  F+  +GV+WD++LV +AYEWVE+ +  +P   
Sbjct: 121 DGIDNVRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQ 180

Query: 184 AWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVA 243
           +WQS   +A LEAGV PDNGF L H  GT+  GS FD+ G RH + ELLNK  P N+KVA
Sbjct: 181 SWQSITGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVA 240

Query: 244 IEATVQRILF----SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGV 303
           +EA VQ+I+F    SGLTA GV+Y+DS G  HRA +  KGE+I+SAG +G+PQLLLLSGV
Sbjct: 241 VEAAVQKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGV 300

Query: 304 GPKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSF 363
           GP+SYL+SL + VV   P++G++++DNPR   NI+ P  + PS+V V+GI  D  + Q  
Sbjct: 301 GPESYLTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCS 360

Query: 364 ASPSPILVPPIFSLLPPQSTSI-IPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFN 423
            S  P   PP FSL P  S  +   + A  V K     S GSL L SS+NV  +P V+FN
Sbjct: 361 LSSLPFDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFN 420

Query: 424 YYSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEE 483
           Y S P DL  CVSG++K+G  L T  ++  K  DL G   F  LG PLPEN  +D++ E+
Sbjct: 421 YCSDPVDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEK 480

Query: 484 YCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           +C  TV +YWHYHGG +VGKV+DGN++V GI  LR+
Sbjct: 481 FCRDTVASYWHYHGGAIVGKVIDGNFRVTGINALRV 513

BLAST of Lsi11G007410 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.7e-139
Identity = 251/515 (48.74%), Postives = 343/515 (66.60%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPN---QDFSYMKFVHNASDLPAKEEYDYIIIG 63
           ME S  ++++  L + +  L     H++ N    DFSY+KFV+NA+D   +  YDYI+IG
Sbjct: 1   MEKSTMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIG 60

Query: 64  GGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSED 123
           GGT+GCPLA TLS  + VLLLERG+   +YP+ L   G        DDGK P +RF SED
Sbjct: 61  GGTSGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSED 120

Query: 124 GVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNA 183
           G++N+R R+LGG +++NAG Y+R +  F+   G++WD++LV K YEWVE+ +  +P   +
Sbjct: 121 GIDNVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQS 180

Query: 184 WQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAI 243
           WQS      LEAG+LPDNGF L H  GT+  GS FD+ G RH A ELLNK  P N+ VA+
Sbjct: 181 WQSVIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAV 240

Query: 244 EATVQRILF----SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVG 303
           +A+V++ILF    S L+A GV+Y+DS G  H+AF+R  GE+IVSAG IG+PQLLLLSGVG
Sbjct: 241 QASVEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVG 300

Query: 304 PKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFA 363
           P+SYLSSL + VV   P++G+F+ +NPR   N   P  +  S V V+GI  D  Y Q   
Sbjct: 301 PESYLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSL 360

Query: 364 SPSPILVPPIFSLLPPQSTSIIPS-LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNY 423
           S  P   PP FSL P  S  +  S  A  V +     S GS+ LNSS++V+ +P ++FNY
Sbjct: 361 SSLPFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNY 420

Query: 424 YSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEY 483
           YS+  DLA CVSG++K+GDLL+T+ +E  K +D+ G   F +LG+PLPEN  +D+S E +
Sbjct: 421 YSNSTDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETF 480

Query: 484 CHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           C   V +YWHYHGG LVGKV+D +++V+GIK LR+
Sbjct: 481 CLDNVASYWHYHGGSLVGKVLDDSFRVMGIKALRV 512

BLAST of Lsi11G007410 vs. ExPASy TrEMBL
Match: A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.8e-264
Identity = 453/507 (89.35%), Postives = 484/507 (95.46%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEHSR TILILSL+ISIFQL + SSHAIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRF SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIRGRVLGGGSMVNAGFYSRGHR+FF +AGVDWDMELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRS+LLE GV+PD+GFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A+GVLYSDSKGKLHRA IR+KGEIIVSAGAIGSPQLLLLSG+GPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++G+ MSDNPRFGTNII+PF +LPSSVKVVGIL+DNIYIQS ASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           P IFSLLPPQ+TSIIP+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL 
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRKMGDLLKT+TMEKIKT++LEGNK FEFLG+PLPENL NDSSVEEYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGCLVGKVVDGNYKVIGIKNLR+
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRV 507

BLAST of Lsi11G007410 vs. ExPASy TrEMBL
Match: A0A5A7VNU1 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001020 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 9.5e-255
Identity = 441/507 (86.98%), Postives = 473/507 (93.29%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEH R TILILSL+ISIFQL + SS AIPNQD  YMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIR RVLGGGSMVNAGFYSRGH++FF +AGVDW+MELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++G+ MSDNPRFGTNIILPF +LPSSVKVVGIL+DNIYIQS ASP PILV
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           PPIFS L  Q TSI P+ A+FVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRK+GDLLKT+TMEKIKTQ+LEGNK FEFLG+PLPENL N S+VEEYC KT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGC+VGKVVDGNYKVIGIKNLRI
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRI 506

BLAST of Lsi11G007410 vs. ExPASy TrEMBL
Match: A0A1S3BSB5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 9.5e-255
Identity = 441/507 (86.98%), Postives = 473/507 (93.29%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEH R TILILSL+ISIFQL + SS AIPNQD  YMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIR RVLGGGSMVNAGFYSRGH++FF +AGVDW+MELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++G+ MSDNPRFGTNIILPF +LPSSVKVVGIL+DNIYIQS ASP PILV
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           PPIFS L  Q TSI P+ A+FVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRK+GDLLKT+TMEKIKTQ+LEGNK FEFLG+PLPENL N S+VEEYC KT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGC+VGKVVDGNYKVIGIKNLRI
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRI 506

BLAST of Lsi11G007410 vs. ExPASy TrEMBL
Match: A0A5A7VGR6 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G001190 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.2e-250
Identity = 431/507 (85.01%), Postives = 467/507 (92.11%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEHSR TILILSL+ISIFQL + SSHAIPNQD SYMKFVHNA+DL  KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLSTKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FSVL+LERGSDPNKYP VLNE GL NVF AGDDGKNP QRF SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLVLERGSDPNKYPFVLNELGLFNVFTAGDDGKNPSQRFISEDGVE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIRGRVLGGGSMVNAGFYSRGHR+FFE+AGVDWDMELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
            FRS+LLE+GV+PDNGFDLRHLVGTKTGGSIFD+ GNRHGAVE LNKA  TNIKVAIEAT
Sbjct: 181 TFRSSLLESGVVPDNGFDLRHLVGTKTGGSIFDNIGNRHGAVEFLNKANTTNIKVAIEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR+KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRKKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLP+VLHQP++G+ MSDNPRFGT  ILPF +LPSS K VGIL+DNIYIQS AS  PIL+
Sbjct: 301 LKLPLVLHQPYVGQSMSDNPRFGTYFILPFPVLPSSAKAVGILQDNIYIQSIASLLPILI 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           PPIFS LPP +TSI PSL + +GKFSEVHSEGSLRLNSST+VK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFLPPGATSIDPSLVVLIGKFSEVHSEGSLRLNSSTDVKRSPIVRFNYYSHPDDLG 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRK+GDLLKT+TMEKIKTQ+LE NK FEFLG+PLPENL N S+VEEYC KTV+TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEDNKGFEFLGVPLPENLGNVSAVEEYCKKTVSTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGC+VGKVVDGNYKVIGIKNLRI
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRI 507

BLAST of Lsi11G007410 vs. ExPASy TrEMBL
Match: A0A1S3BTS5 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 6.2e-246
Identity = 426/508 (83.86%), Postives = 462/508 (90.94%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           ME  + TILILSL ISIF L +LSS AIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MERCKVTILILSLTISIFPLQLLSSEAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FS+L+LERGSDPNKYPSVLN QG+ N FAAGDDGKNPFQ F SEDGVE
Sbjct: 61  AGCPLATTLSSKFSILVLERGSDPNKYPSVLNAQGINNAFAAGDDGKNPFQYFVSEDGVE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPIL-NAWQ 183
           NIRGRVLGGGSMVNAGFYSRGHR+FFE AGVDWDMELVEKAYEWVEETV S+PIL NAWQ
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFENAGVDWDMELVEKAYEWVEETVVSQPILNNAWQ 180

Query: 184 SAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEA 243
           +AFRSALLE GV+PDNGFDLRHLVGTK GGSIFD+KGNRHGAVELLNKA PTNIKVAI+A
Sbjct: 181 AAFRSALLEGGVVPDNGFDLRHLVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVAIQA 240

Query: 244 TVQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLS 303
           TVQ+ILFSGL+A+GV YSDSKGKLH A I + GEIIVSAGAIGSPQLLLLSG+GPKS+LS
Sbjct: 241 TVQKILFSGLSATGVSYSDSKGKLHTASIHKNGEIIVSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 304 SLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPIL 363
           SLKLPVVLHQPH+G+FM+DNPRFGT I+LPF+LLPS+ KVVGIL++NIYIQSFA PS  L
Sbjct: 301 SLKLPVVLHQPHVGQFMTDNPRFGTTIVLPFQLLPSAGKVVGILQENIYIQSFAGPSTFL 360

Query: 364 VPPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDL 423
           VPP FSLLPPQSTSI P+LA FVGKFS+V+S+G L LNSS +VKKSPIVRFNYYSH DDL
Sbjct: 361 VPPAFSLLPPQSTSINPTLASFVGKFSDVNSKGWLHLNSSLDVKKSPIVRFNYYSHHDDL 420

Query: 424 ARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTT 483
            RCV GVRK+GDLLKTQT+EKIKTQDLEG K F FLGLPL ENL NDSSV+EYC KTVTT
Sbjct: 421 DRCVRGVRKVGDLLKTQTIEKIKTQDLEGEKAFMFLGLPLTENLWNDSSVKEYCKKTVTT 480

Query: 484 YWHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           YWHYHGGCLVGKVVDGNYKVIGI+NLR+
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIENLRV 508

BLAST of Lsi11G007410 vs. NCBI nr
Match: XP_038889314.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 927.5 bits (2396), Expect = 5.0e-266
Identity = 459/507 (90.53%), Postives = 481/507 (94.87%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEHS+ TILILSL+ISI QL VL+ +AIP +D  YMKFVH ASDLPAKEEYDYIIIGGGT
Sbjct: 1   MEHSKATILILSLMISISQLGVLTYNAIPIKDVRYMKFVHKASDLPAKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLA TLSS FSVL+LERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDGVE
Sbjct: 61  AGCPLAATLSSKFSVLVLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFISEDGVE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIR RVLGGGSMVNAGFYSRGH+QFFETAGVDWDMELVEKAY+WVEETV SRPILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVASRPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRSALLEAGV+PDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKA+PTNIKVAIEAT
Sbjct: 181 AFRSALLEAGVVPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAEPTNIKVAIEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQ+ILFSGL+A GVLYSDSKGKLHRAFIR+KGEIIVSAGAIGSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQKILFSGLSARGVLYSDSKGKLHRAFIRKKGEIIVSAGAIGSPQLLLLSGVGPKSHLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++GEFMSDNPRFGTNIILPF+L+PSSVKVVGIL+DNIYIQSFASPSP   
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           PP FSLLPPQ  SI+PSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRKMGDLLKTQTMEKIKT DLEGNK F+FLGLPLPENL NDSSV EYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVGEYCQKTVTTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGCLVGKVVDGNYKVIGIKNLR+
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRV 507

BLAST of Lsi11G007410 vs. NCBI nr
Match: XP_011650116.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 922.2 bits (2382), Expect = 2.1e-264
Identity = 454/507 (89.55%), Postives = 484/507 (95.46%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEHSR TILILSL+ISIFQL + SSHAIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FSVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRF SEDGVE
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIRGRVLGGGSMVNAGFYSRGHR+FF +AGVDWDMELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVAIEAT
Sbjct: 181 AFRSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A+GVLYSDSKGKLHRA IR+KGEIIVSAGAIGSPQLLLLSG+GPKS+LSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++G+ MSDNPRFGTNII+PF +LPSSVKVVGIL+DNIYIQS ASP PIL+
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILI 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           P IFSLLPPQ+TSIIP+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL 
Sbjct: 361 PQIFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLG 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRKMGDLLKT+TMEKIKT++LEGNK FEFLG+PLPENL NDSSVEEYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGCLVGKVVDGNYKVIGIKNLR+
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIKNLRV 507

BLAST of Lsi11G007410 vs. NCBI nr
Match: XP_038889315.1 ((R)-mandelonitrile lyase 1-like [Benincasa hispida])

HSP 1 Score: 921.0 bits (2379), Expect = 4.7e-264
Identity = 452/507 (89.15%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEHSR TILILSL+ISIF L +LSSHAIPNQD  YMKFVH+A+DLPAKEEYDYIIIGGGT
Sbjct: 1   MEHSRSTILILSLMISIFHLGLLSSHAIPNQDVGYMKFVHSATDLPAKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPL++TLSS FSVLLLERGSDPNKYPSVLNEQGLLN FAA DDGKNPFQ F SEDGVE
Sbjct: 61  AGCPLSSTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNAFAAEDDGKNPFQHFISEDGVE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIRGRVLGGGSMVN GFYSRGH+QFFETAGVDWDMELVEKAY+WVEETV S+PILNAWQS
Sbjct: 121 NIRGRVLGGGSMVNVGFYSRGHQQFFETAGVDWDMELVEKAYQWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRSA L+AGV+PDNGFDLRH VGTK GGSIFD+KGNRHGAVELLNKA PTNIKV IEAT
Sbjct: 181 AFRSASLDAGVVPDNGFDLRHRVGTKIGGSIFDNKGNRHGAVELLNKANPTNIKVGIEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A GVLYSDSKGKLHRAFIR++GEIIVSAGA+GSPQLLLLSGVGPKS+LSS
Sbjct: 241 VQRILFSGLSARGVLYSDSKGKLHRAFIRKEGEIIVSAGAVGSPQLLLLSGVGPKSHLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++GEFMSDNPRFGTNIILPF+L+PSSVKVVGIL+DNIYIQSFASPSP   
Sbjct: 301 LKLPVVLHQPYVGEFMSDNPRFGTNIILPFQLVPSSVKVVGILQDNIYIQSFASPSPFFA 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           PP FSLLPPQ  SI+PSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA
Sbjct: 361 PPSFSLLPPQFNSIVPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRKMGDLLKTQTMEKIKT DLEGNK F+FLGLPLPENL NDSSVEEYC KTVTTY
Sbjct: 421 RCVKGVRKMGDLLKTQTMEKIKTHDLEGNKGFQFLGLPLPENLSNDSSVEEYCQKTVTTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGCLVGKVVDGNYKVIGI+NLR+
Sbjct: 481 WHYHGGCLVGKVVDGNYKVIGIENLRV 507

BLAST of Lsi11G007410 vs. NCBI nr
Match: KAE8651660.1 (hypothetical protein Csa_021193 [Cucumis sativus])

HSP 1 Score: 902.5 bits (2331), Expect = 1.7e-258
Identity = 442/494 (89.47%), Postives = 472/494 (95.55%), Query Frame = 0

Query: 17  IISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNF 76
           +ISIFQL + SSHAIPNQD SYMKFVHNA+DLP KEEYDYIIIGGGTAGCPLATTLSS F
Sbjct: 1   MISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAGCPLATTLSSKF 60

Query: 77  SVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMV 136
           SVLLLERGSDPNKYPSVL+EQGLLNVFAAGDDG+NPFQRF SEDGVENIRGRVLGGGSMV
Sbjct: 61  SVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENIRGRVLGGGSMV 120

Query: 137 NAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLP 196
           NAGFYSRGHR+FF +AGVDWDMELVEKAYEWVEETV S+PILNAWQSAFRS+LLE GV+P
Sbjct: 121 NAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAFRSSLLEGGVVP 180

Query: 197 DNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILFSGLTASG 256
           DNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVAIEATVQRILFSGL+A+G
Sbjct: 181 DNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQRILFSGLSANG 240

Query: 257 VLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKLPVVLHQPHIG 316
           VLYSDSKGKLHRA IR+KGEIIVSAGAIGSPQLLLLSG+GPKS+LSSLKLPVVLHQP++G
Sbjct: 241 VLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVG 300

Query: 317 EFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILVPPIFSLLPPQSTS 376
           + MSDNPRFGTNII+PF +LPSSVKVVGIL+DNIYIQS ASP PIL+P IFSLLPPQ+TS
Sbjct: 301 QSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQIFSLLPPQATS 360

Query: 377 IIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARCVSGVRKMGDLL 436
           IIP+LAMFVGKFSEVHSEGSLRLNSSTNVKKSPIV FNYYSHPDDL RCV GVRKMGDLL
Sbjct: 361 IIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRCVKGVRKMGDLL 420

Query: 437 KTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTYWHYHGGCLVGKVV 496
           KT+TMEKIKT++LEGNK FEFLG+PLPENL NDSSVEEYC KTVTTYWHYHGGCLVGKVV
Sbjct: 421 KTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWHYHGGCLVGKVV 480

Query: 497 DGNYKVIGIKNLRI 511
           DGNYKVIGIKNLR+
Sbjct: 481 DGNYKVIGIKNLRV 494

BLAST of Lsi11G007410 vs. NCBI nr
Match: XP_008452041.1 (PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo])

HSP 1 Score: 889.0 bits (2296), Expect = 2.0e-254
Identity = 441/507 (86.98%), Postives = 473/507 (93.29%), Query Frame = 0

Query: 4   MEHSRGTILILSLIISIFQLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGT 63
           MEH R TILILSL+ISIFQL + SS AIPNQD  YMKFVHNA+DLP KEEYDYIIIGGGT
Sbjct: 1   MEHFRVTILILSLMISIFQLQLHSSLAIPNQDVHYMKFVHNATDLPTKEEYDYIIIGGGT 60

Query: 64  AGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVE 123
           AGCPLATTLSS FSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRF SEDG+E
Sbjct: 61  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFVSEDGIE 120

Query: 124 NIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQS 183
           NIR RVLGGGSMVNAGFYSRGH++FF +AGVDW+MELVEKAYEWVEETV S+PILNAWQS
Sbjct: 121 NIRARVLGGGSMVNAGFYSRGHQEFFASAGVDWNMELVEKAYEWVEETVVSQPILNAWQS 180

Query: 184 AFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEAT 243
           AFRS+LLE GV+PDNGFDLRHLVGTKTGGSIFD+KGNRHGAVELLNKA PTNIKVA EAT
Sbjct: 181 AFRSSLLEGGVIPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVATEAT 240

Query: 244 VQRILFSGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKSYLSS 303
           VQRILFSGL+A+GVLYSDSKGKLH+AFIR KGEII+SAGAIGSPQLLLLSG+GPKSYLSS
Sbjct: 241 VQRILFSGLSANGVLYSDSKGKLHKAFIRRKGEIILSAGAIGSPQLLLLSGIGPKSYLSS 300

Query: 304 LKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPSPILV 363
           LKLPVVLHQP++G+ MSDNPRFGTNIILPF +LPSSVKVVGIL+DNIYIQS ASP PILV
Sbjct: 301 LKLPVVLHQPYVGQSMSDNPRFGTNIILPFPVLPSSVKVVGILQDNIYIQSIASPFPILV 360

Query: 364 PPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLA 423
           PPIFS L  Q TSI P+ A+FVGKFSEVHSEGSLRLNSSTNVK+SPIVRFNYYSHPDDL 
Sbjct: 361 PPIFSFL-TQVTSITPTFAIFVGKFSEVHSEGSLRLNSSTNVKRSPIVRFNYYSHPDDLG 420

Query: 424 RCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKTVTTY 483
           RCV GVRK+GDLLKT+TMEKIKTQ+LEGNK FEFLG+PLPENL N S+VEEYC KT +TY
Sbjct: 421 RCVKGVRKIGDLLKTKTMEKIKTQNLEGNKGFEFLGVPLPENLGNVSAVEEYCKKTESTY 480

Query: 484 WHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           WHYHGGC+VGKVVDGNYKVIGIKNLRI
Sbjct: 481 WHYHGGCVVGKVVDGNYKVIGIKNLRI 506

BLAST of Lsi11G007410 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.6e-135
Identity = 238/511 (46.58%), Postives = 342/511 (66.93%), Query Frame = 0

Query: 10  TILILSLIISIFQLAVLSSHAIP--NQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCP 69
           ++L  +L++ +    V  S+A P  N+   +M+F+ NA+D  +++ YDYII+GGGTAGCP
Sbjct: 8   SLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCP 67

Query: 70  LATTLSSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRG 129
           LA TLS +F VLLLERG  P   P+V++  G L      ++  +P Q F SE+GV N RG
Sbjct: 68  LAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARG 127

Query: 130 RVLGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRS 189
           RVLGG S +NAGFYSR  +QFFE +G+ WD+  V ++YEWVE  +  RP L  WQ+A R 
Sbjct: 128 RVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRD 187

Query: 190 ALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRI 249
           ALLE GV P NGF L H VGTK GGS FD  G RH + +LL  A+ +NI+VA+ ATV+R+
Sbjct: 188 ALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVERV 247

Query: 250 LF--------SGLTASGVLYSDSKGKLHRAFIREKGEIIVSAGAIGSPQLLLLSGVGPKS 309
           L         S ++A GV+Y D  G+ H A IR++GE+I+SAGA+GSPQLL LSG+GP+S
Sbjct: 248 LLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRS 307

Query: 310 YLSSLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQSFASPS 369
           YLS+  +PV L QPH+G+F+ DNPR G +I+ P  +  S ++VVG+ ED  ++++ ++  
Sbjct: 308 YLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVI 367

Query: 370 PILVPPIFSLLPPQSTSIIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHP 429
           P   P     +   ++ +   +   + K     S G LRL +ST+V+ +P+VRFNY+S P
Sbjct: 368 PFASPLHSVFIRAPASPLYVPVTTIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDP 427

Query: 430 DDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENLLNDSSVEEYCHKT 489
            DL RCV+G RK+G++L+++ M+    ++  GN+ F F+G PLP +  ND  + ++C +T
Sbjct: 428 QDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRT 487

Query: 490 VTTYWHYHGGCLVGKVVDGNYKVIGIKNLRI 511
           V+T WHYHGG +VGKVVD + KVIG+ +LR+
Sbjct: 488 VSTIWHYHGGAVVGKVVDSDLKVIGVNSLRL 517

BLAST of Lsi11G007410 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 381.7 bits (979), Expect = 9.5e-106
Identity = 234/542 (43.17%), Postives = 307/542 (56.64%), Query Frame = 0

Query: 14  LSLIISIF-QLAVLSSHAIPNQDFSYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTL 73
           L + +S+F    + SS   PN       F+ +A+  P    YDYIIIGGGTAGCPLA TL
Sbjct: 10  LFVALSLFLHSPICSSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATL 69

Query: 74  SSNFSVLLLERGSDPNKYPSVLNEQGLLNVFAAGDDG---KNPFQRFTSEDGVENIRGRV 133
           S N SVLLLERG  P   P++      L+ F A        +P QRF SEDGV N R RV
Sbjct: 70  SQNASVLLLERGDSPYNNPNITR----LSAFGAALSDLSESSPSQRFVSEDGVINARARV 129

Query: 134 LGGGSMVNAGFYSRGHRQFFETAGVDWDMELVEKAYEWVEETVTSRPILNAWQSAFRSAL 193
           LGGGS +NAGFY+R   ++    G  WD  L  ++Y+WVE  V  +P +  WQ+A R  L
Sbjct: 130 LGGGSALNAGFYTRAGTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGL 189

Query: 194 LEAGVLPDNGFDLRHLVGTKTGGSIFDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF 253
           LEAG++P+NGF   H+ GTK GG+IFD  GNRH A +LL  A P  I V + ATV RILF
Sbjct: 190 LEAGIVPNNGFTYDHINGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILF 249

Query: 254 --SGLT---ASGVLYSDSKGKLHRAFIRE--KGEIIVSAGAIGSPQLLLLSGVGPKSYLS 313
              G T   A+GV+Y D  G+ HRA+++E    EII+SAG +GSPQLL+LSGVGP + L 
Sbjct: 250 RTRGTTKPIANGVVYRDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQ 309

Query: 314 SLKLPVVLHQPHIGEFMSDNPRFGTNIILPFKLLPSSVKVVGILEDNIYIQ--------- 373
           +  + VV+ QPH+G+ M DNP     +  P  +  S ++VVGI  +  Y++         
Sbjct: 310 AQNITVVMDQPHVGQGMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGG 369

Query: 374 ----SFASPSP----ILVPPIFSLLPPQSTSIIPSLAMFVG-----KFSEVHSEGSLRLN 433
               S  S S      +  P  +LL   S + + S   F G     K     S G L L 
Sbjct: 370 GGGGSSGSSSTRDYYAMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL- 429

Query: 434 SSTNVKKSPIVRFNYYSHPDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEF--- 493
            + N K +PIV FNY+ HPDDL RCV G++ +  +++++   + K  D+     FE+   
Sbjct: 430 KTRNPKDNPIVTFNYFQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADVS----FEYLLN 489

Query: 494 LGLPLPENLLN---------DSSVEEYCHKTVTTYWHYHGGCLVGKVVDGNYKVIGIKNL 511
           L    P NL             S EE+C  TVTT WHYHGGC+VG+VVDG+YKVIGI  L
Sbjct: 490 LTASTPVNLRPPRSGPGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRL 535

BLAST of Lsi11G007410 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 352.1 bits (902), Expect = 8.0e-97
Identity = 218/519 (42.00%), Postives = 292/519 (56.26%), Query Frame = 0

Query: 37  SYMKFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDP--NKYPSVL 96
           S   F  N  D      YDYI+IGGGTAGCPLA TLS NFSVL+LERG  P  N   S L
Sbjct: 50  SSSSFSSNGQD----SSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFL 109

Query: 97  NEQGLLNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGV 156
                 ++  A     +  Q F S DGV N R RVLGGGS +NAGFYSR    F + AG 
Sbjct: 110 RN---FHIGLADISASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG- 169

Query: 157 DWDMELVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSI 216
            WD +LV+++Y WVE  +  +P L  WQ A R +LLE GV P NGF   H+ GTK GG+I
Sbjct: 170 -WDPKLVKESYPWVEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTI 229

Query: 217 FDDKGNRHGAVELLNKAKPTNIKVAIEATVQRILF--SGL--TASGVLYSDSKGKLHRAF 276
           FD  G RH A ELL  A P  ++V I ATVQ+I+F  SG     +GV++ D KG  H+A 
Sbjct: 230 FDRFGRRHTAAELLAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQAL 289

Query: 277 I--REKGEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKLPVVLHQPHIGEFMSDNPRFGTN 336
           +  R+  E+I+S+GAIGSPQ+L+LSG+GPK  L  LK+PVVL   H+G+ M+DNP     
Sbjct: 290 LSNRKGSEVILSSGAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NT 349

Query: 337 IILPFK--LLPSSVKVVGILEDNIYIQSFA----SPSPILV--------PPIFSLLP--- 396
           I++P K  +  S ++ VGI +  +Y+++      SP  I            +FS +P   
Sbjct: 350 ILVPSKAPIEQSLIQTVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQ 409

Query: 397 --PQSTSIIPSL-----------AMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSH 456
             P++T    +            +  + K +   S G L L  +TNV  +P V FNY+ H
Sbjct: 410 RRPEATQAYITRNKYQLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKH 469

Query: 457 PDDLARCVSGVRKMGDLLKTQTMEKIKTQDLEGNKVFEFLGLPLPENL------LNDS-S 511
           P DL RCV  +R +  ++ +     +     +   V + L L +  N+      LND+ S
Sbjct: 470 PVDLQRCVEAIRLVSKVVTSNRF--LNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKS 529

BLAST of Lsi11G007410 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 345.9 bits (886), Expect = 5.8e-95
Identity = 205/504 (40.67%), Postives = 288/504 (57.14%), Query Frame = 0

Query: 41  FVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGLL 100
           F+ +A+  P    +DYIIIGGGTAGC LA TLS N SVL+LERG  P + P+  +    +
Sbjct: 60  FMKDATLAPKNASFDYIIIGGGTAGCALAATLSQNASVLVLERGGSPYENPTATDMGNSV 119

Query: 101 NVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDMEL 160
           N     +   +  Q F SEDGV N R RVLGGGS++N GFYSR    + E A  +W+ME 
Sbjct: 120 NTL-LNNTPNSWSQLFISEDGVYNTRPRVLGGGSVINGGFYSRAGNDYVEEA--EWEMEE 179

Query: 161 VEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKGN 220
           VE AYEWVE+ +   P +  WQ AF+  LLEAG  PDNGF   H+ GTK GG+IFD  G+
Sbjct: 180 VEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEAGESPDNGFTYDHIYGTKIGGTIFDRAGH 239

Query: 221 RHGAVELLNKAKPTNIKVAIEATVQRILFSGLTASGVLYSDSKGKLHRAFIREK--GEII 280
           RH A  LL  A P  I V + A+V ++LF+   A  VL+ D+ G  H+A +  K   E+I
Sbjct: 240 RHTAANLLEYANPNRIVVYLHASVHKVLFT-TEAYEVLFEDANGVFHKANLANKATNEVI 299

Query: 281 VSAGAIGSPQLLLLSGVGPKSYLSSLKL-PVVLHQPHIGEFMSDNPRFGTNIILPFKLLP 340
           +SAGA+GSPQLL+LSGVGP  +L +  + P+VL QP +G+ M+DNP     I  P  +  
Sbjct: 300 LSAGALGSPQLLMLSGVGPAVHLEAHGVNPLVLDQPMVGQGMADNPMNFVAIPSPQPVEL 359

Query: 341 SSVKVVGILEDNIYIQSFASPS------PILVPPIFSLLPPQSTS-----IIPSLAMFVG 400
           S ++ VGI + + YI+  +  S            + +LL   S +     +  S+A+ + 
Sbjct: 360 SLIQAVGITKFDSYIEGLSGLSLSFDITRRFFDGVLNLLNETSHTTSRKILTQSIAVLLK 419

Query: 401 KFS---EVHSEGSLRLNS-------------STNVKKSPIVRFNYYSHPDDLARCVSGVR 460
            F    EV   G L                 +TN + +P V FNYY  P+DL +CV G+ 
Sbjct: 420 SFDVKLEVRMNGGLIFQKVDGPASKGHMKLRNTNPRDNPSVTFNYYQEPEDLNKCVKGLN 479

Query: 461 KMGDLLKTQTMEKIKTQDLEGNKVFE-FLGLPL---PENLLNDSSVEEYCHKTVTTYWHY 511
            +  ++ ++   K K   +   ++    L LP+   P ++ +  +++++C  TVT+ WHY
Sbjct: 480 TIIRMINSKAFSKYKYPGVTARELLNLMLALPINLRPRHVTSAFNLKQFCIDTVTSVWHY 539

BLAST of Lsi11G007410 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 339.7 bits (870), Expect = 4.1e-93
Identity = 211/507 (41.62%), Postives = 284/507 (56.02%), Query Frame = 0

Query: 40  KFVHNASDLPAKEEYDYIIIGGGTAGCPLATTLSSNFSVLLLERGSDPNKYPSVLNEQGL 99
           +F+ +A+  P    +DYIIIGGGTAGC LA TLS N +VL+LERG  P   P+  +    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATDIGNF 91

Query: 100 LNVFAAGDDGKNPFQRFTSEDGVENIRGRVLGGGSMVNAGFYSRGHRQFFETAGVDWDME 159
            N         +  Q F SEDGV N R RVLGGG+++NAGFYSR    F   AG  W+ +
Sbjct: 92  ANTL-LNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--WERD 151

Query: 160 LVEKAYEWVEETVTSRPILNAWQSAFRSALLEAGVLPDNGFDLRHLVGTKTGGSIFDDKG 219
            VE AYEWVE+ V   P +N WQSAFR  LLEAGV P NGF   H+VGTK GG+IFD  G
Sbjct: 152 EVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFDRDG 211

Query: 220 NRHGAVELLNKAKPTNIKVAIEATVQRILFS-----GLTASGVLYSDSKGKLHRAFIREK 279
           +RH A  LL  A P  I V + A+V +ILF+        A GV++ D+ G  ++A +  +
Sbjct: 212 HRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAELATQ 271

Query: 280 ----GEIIVSAGAIGSPQLLLLSGVGPKSYLSSLKL-PVVLHQPHIGEFMSDNPRFGTNI 339
                E+I+SAGAI SPQLL+LSGVGP ++L++ ++ PV++ QP +G+ M DNP     I
Sbjct: 272 DSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNPVFI 331

Query: 340 ILPFKLLPSSVKVVGILEDNIYIQ--SFASPSPILVPPIF---------SLLPPQSTS-- 399
             P  +  S V+ VGI +   YI+  S  S S  L    F         + LP QS S  
Sbjct: 332 PSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSISKF 391

Query: 400 ---------IIPSLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVRFNYYSHPDDLARCVS 459
                    +     + + K +   S G L L  +TN   +P V FNY+  P+DL +CV 
Sbjct: 392 FKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NTNPDDNPSVTFNYFKDPEDLNKCVE 451

Query: 460 GVRKMGDLLKTQTMEKIKTQDLEGNKVFE-FLGLPL---PENLLNDSSVEEYCHKTVTTY 511
           G+  +  ++ ++   K K        +    L LP    P ++ +   +E+YC  TV T 
Sbjct: 452 GLSTIIKVIDSKGYSKYKYPLASARGLLNLILALPTNLRPRHITSTFDLEQYCIDTVMTI 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527061.3e-14751.68(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K23.5e-14550.00(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527071.4e-14149.41(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O827844.0e-14149.42(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
O242431.7e-13948.74(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LK763.8e-26489.35Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1[more]
A0A5A7VNU19.5e-25586.98(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BSB59.5e-25586.98(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493164 PE=3 SV=... [more]
A0A5A7VGR63.2e-25085.01(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S3BTS56.2e-24683.86(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493179 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
XP_038889314.15.0e-26690.53(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
XP_011650116.22.1e-26489.55(R)-mandelonitrile lyase 1 [Cucumis sativus][more]
XP_038889315.14.7e-26489.15(R)-mandelonitrile lyase 1-like [Benincasa hispida][more]
KAE8651660.11.7e-25889.47hypothetical protein Csa_021193 [Cucumis sativus][more]
XP_008452041.12.0e-25486.98PREDICTED: (R)-mandelonitrile lyase 1-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT1G73050.12.6e-13546.58Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.19.5e-10643.17Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.18.0e-9742.00Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51930.15.8e-9540.67Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.14.1e-9341.62Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.410.40coord: 174..482
e-value: 6.4E-156
score: 522.1
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 8..510
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 8..510
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 392..489
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 54..322
e-value: 1.2E-31
score: 110.2
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 127..150
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 282..296
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 54..510
e-value: 6.4E-156
score: 522.1
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 34..355
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 367..511
e-value: 1.0E-18
score: 65.4
coord: 34..340
e-value: 1.1E-54
score: 184.1
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 392..511
e-value: 6.2E-18
score: 65.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G007410.1Lsi11G007410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors