Lsi11G006340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G006340
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	nicotianamine synthase-like
Location	chr11: 6700569 .. 6701024 (+)
RNA-Seq Expression	Lsi11G006340
Synteny	Lsi11G006340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTTGAAGAAGCCCCTCTCTTGCAAAAAGTTTGTGCTCTTTACCATGAAATCTCAAGCCTCACTAGCCTTAAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCGCCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGACTCATCTCATTCGTCTTTGTGCTAAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCTTCTTTTCATAATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTGTCGTCGTATTCCTGCTACCGTCGCGTTCGTTGGCTCCGGTCCCCTCCCGCTCTCCTCCATTGTCATGGCGTCCATTCATCTTAAGGGTAACCGTTGTTGA

mRNA sequence

ATGGAATTTGAAGAAGCCCCTCTCTTGCAAAAAGTTTGTGCTCTTTACCATGAAATCTCAAGCCTCACTAGCCTTAAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCGCCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGACTCATCTCATTCGTCTTTGTGCTAAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCTTCTTTTCATAATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTGTCGTCGTATTCCTGCTACCGTCGCGTTCGTTGGCTCCGGTCCCCTCCCGCTCTCCTCCATTGTCATGGCGTCCATTCATCTTAAGGGTAACCGTTGTTGA

Coding sequence (CDS)

ATGGAATTTGAAGAAGCCCCTCTCTTGCAAAAAGTTTGTGCTCTTTACCATGAAATCTCAAGCCTCACTAGCCTTAAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCGCCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGACTCATCTCATTCGTCTTTGTGCTAAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCTTCTTTTCATAATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTTTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTGTCGTCGTATTCCTGCTACCGTCGCGTTCGTTGGCTCCGGTCCCCTCCCGCTCTCCTCCATTGTCATGGCGTCCATTCATCTTAAGGGTAACCGTTGTTGA

Protein sequence

MEFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPATVAFVGSGPLPLSSIVMASIHLKGNRC

Homology

[Close]

BLAST of Lsi11G006340 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.6e-39
Identity = 80/145 (55.17%), Postives = 110/145 (75.86%), Query Frame = 0


Query: 4   EEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRT 63
           ++  L+ K+C LY +IS L +LKP ++VDTLF QLV TC PP P +D+  +S+ +Q MR+
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRS 64

Query: 64  HLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRR-I 123
           +LI++C +AE  LE HFSSIL S   NP+ +L++FPYY+NYLKLS LEFD+L  +    +
Sbjct: 65  NLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV 124

Query: 124 PATVAFVGSGPLPLSSIVMASIHLK 148
           P TVAF+GSGPLPL+S+V+AS HLK
Sbjct: 125 PRTVAFIGSGPLPLTSVVLASSHLK 149

[Close]

BLAST of Lsi11G006340 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 8.1e-39
Identity = 80/141 (56.74%), Postives = 109/141 (77.30%), Query Frame = 0


Query: 7   PLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTHLI 66
           P+++KVC LY +IS L +L PSK+V+ LFT LV TC PP P +D++ L Q +Q +R+HLI
Sbjct: 8   PVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-IDVSKLCQKIQEIRSHLI 67

Query: 67  RLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPATV 126
           +LC +AE LLE HFS IL SS+ NP+ +L IFPY+ NY+KLSLLE++IL  +   IP  +
Sbjct: 68  KLCGQAEGLLESHFSKIL-SSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKI 127

Query: 127 AFVGSGPLPLSSIVMASIHLK 148
           AF+GSGPLPL+S+V+A+ HLK
Sbjct: 128 AFIGSGPLPLTSLVLATKHLK 146

[Close]

BLAST of Lsi11G006340 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 5.8e-37
Identity = 78/147 (53.06%), Postives = 106/147 (72.11%), Query Frame = 0


Query: 1   MEFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASL-SQPLQ 60
           M  E   +++++  LY++IS+L SLKPSKNVDTLF QLV TC P    +D+  +  + ++
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVK 60

Query: 61  AMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHC 120
            MR+HLI+LC +AE  LE HFS+IL S   NP+++L IFPYY+NYLKL  LEFD+L  H 
Sbjct: 61  DMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHT 120

Query: 121 RRIPATVAFVGSGPLPLSSIVMASIHL 147
             +P  VAF+GSGP+PL+SIV+A  HL
Sbjct: 121 THVPTKVAFIGSGPMPLTSIVLAKFHL 147

[Close]

BLAST of Lsi11G006340 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 9.9e-37
Identity = 79/148 (53.38%), Postives = 106/148 (71.62%), Query Frame = 0


Query: 1   MEFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  ++  L+Q +C LY +IS L SLKPS++V+ LF QLV TC PP P +D+  +   +Q 
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQE 60

Query: 61  MRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCR 120
           +R +LI++C  AE  LE HFSSIL S   NP+ +L+IFPYY+NYLKL  LEFD+L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 120

Query: 121 R-IPATVAFVGSGPLPLSSIVMASIHLK 148
             +P +VAF+GSGPLPL+SIV+AS HLK
Sbjct: 121 GFVPKSVAFIGSGPLPLTSIVLASFHLK 148

[Close]

BLAST of Lsi11G006340 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 8.3e-36
Identity = 76/141 (53.90%), Postives = 101/141 (71.63%), Query Frame = 0


Query: 8   LLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTHLIR 67
           +++++  LY +IS L SLKPSKNVDTLF QLV TC P    +D+ ++ + ++ MR +LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 68  LCAKAEALLELHFSSILAS--SFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPAT 127
           LC +AE  LE HFS+IL S     NP+ +L IFPYYSNYLKL  LEFD+L  H   +P  
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 128 VAFVGSGPLPLSSIVMASIHL 147
           +AFVGSGP+PL+SIV+A  HL
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHL 148

[Close]

BLAST of Lsi11G006340 vs. ExPASy TrEMBL
Match: A0A1S3BWE4 (probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.6e-61
Identity = 130/153 (84.97%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 2   EFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLSQP 61
           E +EA LLQKVCALY++ISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASLSQP
Sbjct: 9   EQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQP 68

Query: 62  LQAMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRS 121
           LQAMR HLI+LCAKAEALLELHFSS+LASSFH+PISNLSIFPYYSNYLKLSLLEFDILRS
Sbjct: 69  LQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRS 128

Query: 122 HCRRIPATVAFVGSGPLPLSSIVMASIHLKGNR 151
           H RR+P  VAFVGSGPLPLSSIV+ASIHLKGNR
Sbjct: 129 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNR 161

[Close]

BLAST of Lsi11G006340 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 6.0e-61
Identity = 126/144 (87.50%), Postives = 133/144 (92.36%), Query Frame = 0


Query: 5   EAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTH 64
           E PLL++VCALY +ISSL SLKPSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMR H
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPA 124
           LIRLCA AEALLE+HFSSILA SF NPI NLSIFPYYSNYLKLSLLEFDIL SHCRR+P+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFVGSGPLPLSSIVMASIHLKG 149
           +VAFVGSGPLPLSSIVMAS HLKG
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKG 144

[Close]

BLAST of Lsi11G006340 vs. ExPASy TrEMBL
Match: A0A6J1KIA7 (nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.3e-60
Identity = 125/144 (86.81%), Postives = 133/144 (92.36%), Query Frame = 0


Query: 5   EAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTH 64
           E PLL++VCALY +ISSL SLKPSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMR H
Sbjct: 32  EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 91

Query: 65  LIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPA 124
           LIRLCA AEALLE+HFSSILA SF NPI NLSIFPYYSNYLKLSLLEFDIL SHCRR+P+
Sbjct: 92  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 151

Query: 125 TVAFVGSGPLPLSSIVMASIHLKG 149
           ++AFVGSGPLPLSSIVMAS HLKG
Sbjct: 152 SIAFVGSGPLPLSSIVMASTHLKG 174

[Close]

BLAST of Lsi11G006340 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.1e-59
Identity = 128/151 (84.77%), Postives = 138/151 (91.39%), Query Frame = 0


Query: 2   EFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLSQP 61
           E +EA LLQKVCALY++ISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASLSQP
Sbjct: 9   EQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQP 68

Query: 62  LQAMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRS 121
           LQAMR HLI+LCAKAEALLELHFSS+LASSFH+PISNLSIFPYYSNYLKLSLLEFDILRS
Sbjct: 69  LQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRS 128

Query: 122 HCRRIPATVAFVGSGPLPLSSIVMASIHLKG 149
           H RR+P  VAFVGSGPLPLSSIV+ASIHLKG
Sbjct: 129 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKG 159

[Close]

BLAST of Lsi11G006340 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.1e-59
Identity = 129/154 (83.77%), Postives = 139/154 (90.26%), Query Frame = 0


Query: 1   MEFE---EAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCS---PPAPGLDIASL 60
           MEFE   EA LL+KVCALY++ISSL+SLKP KNVDTLFTQLVLTCS   PP  G DIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLQAMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPL+AMR HLI+LCA+AEALLELHFSS+LASSFH+PISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHCRRIPATVAFVGSGPLPLSSIVMASIHLKG 149
           LRSH RRIP  VAFVGSGPLPLSSIV+ASIHLKG
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKG 154

[Close]

BLAST of Lsi11G006340 vs. NCBI nr
Match: XP_038890564.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 1.4e-67
Identity = 137/149 (91.95%), Postives = 143/149 (95.97%), Query Frame = 0


Query: 1   MEFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAP-GLDIASLSQPLQ 60
           MEFEEAPLLQK+ ALYH+ISSLTSL PSKNVDTLFTQLVLTCSPPAP G DIASLSQPLQ
Sbjct: 1   MEFEEAPLLQKISALYHQISSLTSLNPSKNVDTLFTQLVLTCSPPAPAGFDIASLSQPLQ 60

Query: 61  AMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHC 120
           AMR HLIRLCAKAEALLELHFSSILASSF NPISNLSIFPYYSNYLKLSLLEFDILRSHC
Sbjct: 61  AMRAHLIRLCAKAEALLELHFSSILASSFRNPISNLSIFPYYSNYLKLSLLEFDILRSHC 120

Query: 121 RRIPATVAFVGSGPLPLSSIVMASIHLKG 149
           RR+PA++AF+GSGPLPLSSIVMASIHLKG
Sbjct: 121 RRVPASIAFLGSGPLPLSSIVMASIHLKG 149

[Close]

BLAST of Lsi11G006340 vs. NCBI nr
Match: XP_008453473.1 (PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo])

HSP 1 Score: 243.8 bits (621), Expect = 9.4e-61
Identity = 130/153 (84.97%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 2   EFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLSQP 61
           E +EA LLQKVCALY++ISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASLSQP
Sbjct: 9   EQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQP 68

Query: 62  LQAMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRS 121
           LQAMR HLI+LCAKAEALLELHFSS+LASSFH+PISNLSIFPYYSNYLKLSLLEFDILRS
Sbjct: 69  LQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRS 128

Query: 122 HCRRIPATVAFVGSGPLPLSSIVMASIHLKGNR 151
           H RR+P  VAFVGSGPLPLSSIV+ASIHLKGNR
Sbjct: 129 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNR 161

[Close]

BLAST of Lsi11G006340 vs. NCBI nr
Match: XP_022946411.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 243.4 bits (620), Expect = 1.2e-60
Identity = 126/144 (87.50%), Postives = 133/144 (92.36%), Query Frame = 0


Query: 5   EAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTH 64
           E PLL++VCALY +ISSL SLKPSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMR H
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPA 124
           LIRLCA AEALLE+HFSSILA SF NPI NLSIFPYYSNYLKLSLLEFDIL SHCRR+P+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFVGSGPLPLSSIVMASIHLKG 149
           +VAFVGSGPLPLSSIVMAS HLKG
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKG 144

[Close]

BLAST of Lsi11G006340 vs. NCBI nr
Match: KAG6599185.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 243.4 bits (620), Expect = 1.2e-60
Identity = 126/144 (87.50%), Postives = 133/144 (92.36%), Query Frame = 0


Query: 5   EAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTH 64
           E PLL++VCALY +ISSL SLKPSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMR H
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPA 124
           LIRLCA AEALLE+HFSSILA SF NPI NLSIFPYYSNYLKLSLLEFDIL SHCRR+P+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFVGSGPLPLSSIVMASIHLKG 149
           +VAFVGSGPLPLSSIVMAS HLKG
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKG 144

[Close]

BLAST of Lsi11G006340 vs. NCBI nr
Match: XP_023545298.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 243.4 bits (620), Expect = 1.2e-60
Identity = 126/144 (87.50%), Postives = 133/144 (92.36%), Query Frame = 0


Query: 5   EAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTH 64
           E PLL++VCALY +ISSL SLKPSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMR H
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPA 124
           LIRLCA AEALLE+HFSSILA SF NPI NLSIFPYYSNYLKLSLLEFDIL SHCRR+P+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFVGSGPLPLSSIVMASIHLKG 149
           +VAFVGSGPLPLSSIVMAS HLKG
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKG 144

[Close]

BLAST of Lsi11G006340 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 163.7 bits (413), Expect = 1.2e-40
Identity = 80/145 (55.17%), Postives = 110/145 (75.86%), Query Frame = 0


Query: 4   EEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRT 63
           ++  L+ K+C LY +IS L +LKP ++VDTLF QLV TC PP P +D+  +S+ +Q MR+
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRS 64

Query: 64  HLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRR-I 123
           +LI++C +AE  LE HFSSIL S   NP+ +L++FPYY+NYLKLS LEFD+L  +    +
Sbjct: 65  NLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV 124

Query: 124 PATVAFVGSGPLPLSSIVMASIHLK 148
           P TVAF+GSGPLPL+S+V+AS HLK
Sbjct: 125 PRTVAFIGSGPLPLTSVVLASSHLK 149

[Close]

BLAST of Lsi11G006340 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 155.2 bits (391), Expect = 4.1e-38
Identity = 78/147 (53.06%), Postives = 106/147 (72.11%), Query Frame = 0


Query: 1   MEFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASL-SQPLQ 60
           M  E   +++++  LY++IS+L SLKPSKNVDTLF QLV TC P    +D+  +  + ++
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVK 60

Query: 61  AMRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHC 120
            MR+HLI+LC +AE  LE HFS+IL S   NP+++L IFPYY+NYLKL  LEFD+L  H 
Sbjct: 61  DMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHT 120

Query: 121 RRIPATVAFVGSGPLPLSSIVMASIHL 147
             +P  VAF+GSGP+PL+SIV+A  HL
Sbjct: 121 THVPTKVAFIGSGPMPLTSIVLAKFHL 147

[Close]

BLAST of Lsi11G006340 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 154.5 bits (389), Expect = 7.0e-38
Identity = 79/148 (53.38%), Postives = 106/148 (71.62%), Query Frame = 0


Query: 1   MEFEEAPLLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  ++  L+Q +C LY +IS L SLKPS++V+ LF QLV TC PP P +D+  +   +Q 
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQE 60

Query: 61  MRTHLIRLCAKAEALLELHFSSILASSFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCR 120
           +R +LI++C  AE  LE HFSSIL S   NP+ +L+IFPYY+NYLKL  LEFD+L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 120

Query: 121 R-IPATVAFVGSGPLPLSSIVMASIHLK 148
             +P +VAF+GSGPLPL+SIV+AS HLK
Sbjct: 121 GFVPKSVAFIGSGPLPLTSIVLASFHLK 148

[Close]

BLAST of Lsi11G006340 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 151.4 bits (381), Expect = 5.9e-37
Identity = 76/141 (53.90%), Postives = 101/141 (71.63%), Query Frame = 0


Query: 8   LLQKVCALYHEISSLTSLKPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRTHLIR 67
           +++++  LY +IS L SLKPSKNVDTLF QLV TC P    +D+ ++ + ++ MR +LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 68  LCAKAEALLELHFSSILAS--SFHNPISNLSIFPYYSNYLKLSLLEFDILRSHCRRIPAT 127
           LC +AE  LE HFS+IL S     NP+ +L IFPYYSNYLKL  LEFD+L  H   +P  
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 128 VAFVGSGPLPLSSIVMASIHL 147
           +AFVGSGP+PL+SIV+A  HL
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHL 148

The following BLAST results are available for this feature:

BLAST of Lsi11G006340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7X5	1.6e-39	55.17	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9XGI7	8.1e-39	56.74	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9FKT9	5.8e-37	53.06	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
O80483	9.9e-37	53.38	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	8.3e-36	53.90	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]

BLAST of Lsi11G006340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BWE4	4.6e-61	84.97	probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1G3L4	6.0e-61	87.50	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S...	[more]
A0A6J1KIA7	1.3e-60	86.81	nicotianamine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111493502 PE=3 SV=...	[more]
A0A5D3BBH0	1.1e-59	84.77	Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194...	[more]
A0A0A0LGI4	1.1e-59	83.77	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1	[more]

BLAST of Lsi11G006340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890564.1	1.4e-67	91.95	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_008453473.1	9.4e-61	84.97	PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo]	[more]
XP_022946411.1	1.2e-60	87.50	nicotianamine synthase-like [Cucurbita moschata]	[more]
KAG6599185.1	1.2e-60	87.50	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023545298.1	1.2e-60	87.50	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lsi11G006340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	1.2e-40	55.17	nicotianamine synthase 4	[more]
AT5G56080.1	4.1e-38	53.06	nicotianamine synthase 2	[more]
AT1G09240.1	7.0e-38	53.38	nicotianamine synthase 3	[more]
AT5G04950.1	5.9e-37	53.90	nicotianamine synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 9..151 e-value: 7.0E-21 score: 76.5
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 5..147 e-value: 1.0E-54 score: 185.7
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 4..148
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..151 score: 28.906841

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G006340.1	Lsi11G006340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity