Lsi11G006330 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G006330
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	nicotianamine synthase
Location	chr11: 6689014 .. 6689847 (+)
RNA-Seq Expression	Lsi11G006330
Synteny	Lsi11G006330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTGCCAAGGTCTTCTTTTGGTTCAAAAAATCTCTGAACTTTACCACAAAATCTCCACTCTTGAAAGCCTCAAGCCCTCTAAAGATGTCAACTCTCTCTTCTCTCAACTAGTCCTCATTTGCACCCCTCCCCCGTCTTCTCCGTCGTCCATCGACATCTCGACGCTCTGCTCCACTGTCCAAACCATGAGATCCCATCTCATCAAGCTTTGCGGCGAAGCCGAGGCCCTTCTCGAGCATCACTACTCCACCATTTTAGCTTCTCACCAAAACCCACTTGACCATCTCTCCCTTTTCCCTTATTACTCCAACTACCTCAAACTAACCCACCTTGAATTCTCCATTTTGAACCACCACGCGCCCCACGCGCCACCCTCCAAGGTGGCCTTTGTTGGCTCTGGTGCACTTCCATTCTCGTCGATCATCATGGCCTTGAAGTACCTCCCGAAAGCCGAGTTTCACAACTTCGACGTCGACTCGTCTGCTAATGCCAAAGCAGCGATGCTGGTAGCTGGCGACGCAGACCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTTGCCTCGAATGAGTTGAGGCAGTTTCAGGTGGTGTTTTTGGCGGCCTTGGTGGGGATGGAAAAGGAAGAGAAGGTGAAGGTGATTGAGCATTTGAGGAAGAACATGGGTGGTGGCGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGCGCGTTTCTTTATCCGGTGGTTGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACGGTGTTTCATCCCACGGATGAAGTTATTAATTCAATTGTATTGGCACGATTATAA

mRNA sequence

ATGTGTTGCCAAGGTCTTCTTTTGGTTCAAAAAATCTCTGAACTTTACCACAAAATCTCCACTCTTGAAAGCCTCAAGCCCTCTAAAGATGTCAACTCTCTCTTCTCTCAACTAGTCCTCATTTGCACCCCTCCCCCGTCTTCTCCGTCGTCCATCGACATCTCGACGCTCTGCTCCACTGTCCAAACCATGAGATCCCATCTCATCAAGCTTTGCGGCGAAGCCGAGGCCCTTCTCGAGCATCACTACTCCACCATTTTAGCTTCTCACCAAAACCCACTTGACCATCTCTCCCTTTTCCCTTATTACTCCAACTACCTCAAACTAACCCACCTTGAATTCTCCATTTTGAACCACCACGCGCCCCACGCGCCACCCTCCAAGGTGGCCTTTGTTGGCTCTGGTGCACTTCCATTCTCGTCGATCATCATGGCCTTGAAGTACCTCCCGAAAGCCGAGTTTCACAACTTCGACGTCGACTCGTCTGCTAATGCCAAAGCAGCGATGCTGGTAGCTGGCGACGCAGACCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTTGCCTCGAATGAGTTGAGGCAGTTTCAGGTGGTGTTTTTGGCGGCCTTGGTGGGGATGGAAAAGGAAGAGAAGGTGAAGGTGATTGAGCATTTGAGGAAGAACATGGGTGGTGGCGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGCGCGTTTCTTTATCCGGTGGTTGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACGGTGTTTCATCCCACGGATGAAGTTATTAATTCAATTGTATTGGCACGATTATAA

Coding sequence (CDS)

ATGTGTTGCCAAGGTCTTCTTTTGGTTCAAAAAATCTCTGAACTTTACCACAAAATCTCCACTCTTGAAAGCCTCAAGCCCTCTAAAGATGTCAACTCTCTCTTCTCTCAACTAGTCCTCATTTGCACCCCTCCCCCGTCTTCTCCGTCGTCCATCGACATCTCGACGCTCTGCTCCACTGTCCAAACCATGAGATCCCATCTCATCAAGCTTTGCGGCGAAGCCGAGGCCCTTCTCGAGCATCACTACTCCACCATTTTAGCTTCTCACCAAAACCCACTTGACCATCTCTCCCTTTTCCCTTATTACTCCAACTACCTCAAACTAACCCACCTTGAATTCTCCATTTTGAACCACCACGCGCCCCACGCGCCACCCTCCAAGGTGGCCTTTGTTGGCTCTGGTGCACTTCCATTCTCGTCGATCATCATGGCCTTGAAGTACCTCCCGAAAGCCGAGTTTCACAACTTCGACGTCGACTCGTCTGCTAATGCCAAAGCAGCGATGCTGGTAGCTGGCGACGCAGACCTGTCGAGGCGGATGGTGTTCCACACGACCGACATCATGGACGTTGCCTCGAATGAGTTGAGGCAGTTTCAGGTGGTGTTTTTGGCGGCCTTGGTGGGGATGGAAAAGGAAGAGAAGGTGAAGGTGATTGAGCATTTGAGGAAGAACATGGGTGGTGGCGCGTTGTTGATGTTGAGGAGCGCATATGGTGCACGCGCGTTTCTTTATCCGGTGGTTGAAGCTTGTGATCTTCGAGGGTTTGAGATTCTTACGGTGTTTCATCCCACGGATGAAGTTATTAATTCAATTGTATTGGCACGATTATAA

Protein sequence

MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL

Homology

[Close]

BLAST of Lsi11G006330 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 6.6e-87
Identity = 163/279 (58.42%), Postives = 219/279 (78.49%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           M CQ  L+V++I +LY +IS L+SLKPSK+V++LF QLV  C P   + ++ID++ +C  
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLP---TDTNIDVTNMCEE 60

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILAS---HQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           V+ MR++LIKLCGEAE  LE H+STIL S    QNPLDHL +FPYYSNYLKL  LEF +L
Sbjct: 61  VKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL 120

Query: 121 NHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADL 180
           + H+ H  P+K+AFVGSG +P +SI++A  +LP   FHNFD+DS AN  A+ LV+ D DL
Sbjct: 121 SQHSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDL 180

Query: 181 SRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSA 240
           S+RM+FHTTD+++ A+  L Q+ VVFLAALVGM+KE KVK IEHL K+M  GA+LMLRSA
Sbjct: 181 SKRMIFHTTDVLN-ATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           +  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 HALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Lsi11G006330 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.1e-86
Identity = 167/275 (60.73%), Postives = 210/275 (76.36%), Query Frame = 0


Query: 3   CQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCSTVQ 62
           CQ   LV KI +LY KIS LE+LKP +DV++LF QLV  C PP     +ID++ +   +Q
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPP---NPNIDVTKMSENIQ 63

Query: 63  TMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHA 122
            MRS+LIK+CGEAE  LEHH+S+IL S + NPL HL+LFPYY+NYLKL+ LEF +L  + 
Sbjct: 64  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 123

Query: 123 PHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRM 182
               P  VAF+GSG LP +S+++A  +L  + FHNFD+D SAN  AA LV+ D DLS+RM
Sbjct: 124 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 183

Query: 183 VFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYGAR 242
            FHT DIMDV +  L+ F VVFLAALVGM+K+EKVKV+EHL K+M  GALLMLRSA+G R
Sbjct: 184 FFHTVDIMDV-TESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPR 243

Query: 243 AFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           AFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 AFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Lsi11G006330 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-85
Identity = 161/278 (57.91%), Postives = 219/278 (78.78%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTL-CS 60
           M C+  L+V++I +LY++IS LESLKPSK+V++LF QLV  C P   + ++ID++ +   
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLP---TDTNIDVTEIHDE 60

Query: 61  TVQTMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILN 120
            V+ MRSHLIKLCGEAE  LE H+S IL S + NPL+HL +FPYY+NYLKL  LEF +L+
Sbjct: 61  KVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLS 120

Query: 121 HHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLS 180
            H  H  P+KVAF+GSG +P +SI++A  +LP   FHNFD+DS AN  A+ LV+ D+DLS
Sbjct: 121 QHTTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLS 180

Query: 181 RRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAY 240
           +RM+FHTTD+++ A   L Q+ VVFLAALVGM+KE KVK IEHL K+M  GA++MLRSA+
Sbjct: 181 KRMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAH 240

Query: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 GLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Lsi11G006330 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.6e-85
Identity = 165/277 (59.57%), Postives = 212/277 (76.53%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           M CQ   LVQ I +LY KIS LESLKPS+DVN LF QLV  C PP     +ID++ +C  
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPP---NPNIDVTKMCDR 60

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNH 120
           VQ +R +LIK+CG AE  LE+H+S+IL S+Q NPL HL++FPYY+NYLKL  LEF +L  
Sbjct: 61  VQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQ 120

Query: 121 HAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSR 180
           +     P  VAF+GSG LP +SI++A  +L    FHNFD+D SAN+ A++LV+ D D+S+
Sbjct: 121 NLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 181 RMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYG 240
           RM FHT DIMDV +  L+ F VVFLAALVGM KEEKVKVIEHL+K+M  GA+LMLRSA+G
Sbjct: 181 RMFFHTVDIMDV-TESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHG 240

Query: 241 ARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
            RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 PRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Lsi11G006330 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.2e-84
Identity = 158/276 (57.25%), Postives = 209/276 (75.72%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           +C     +V+K+ ELY +IS LE+L PSKDVN LF+ LV  C P    P+ ID+S LC  
Sbjct: 2   VCPNSNPVVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMP----PNPIDVSKLCQK 61

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120
           +Q +RSHLIKLCG+AE LLE H+S IL+S++NPL HL +FPY+ NY+KL+ LE++IL  +
Sbjct: 62  IQEIRSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKN 121

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRR 180
             +  P K+AF+GSG LP +S+++A K+L    FHN+D+D  AN  A+ LVA D D+S R
Sbjct: 122 TTNI-PKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSR 181

Query: 181 MVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYGA 240
           M FHT D+MDV    L+ + VVFLAALVGM+KE+KVKV++HL K M  GA LMLRSA+GA
Sbjct: 182 MTFHTADVMDVTC-ALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGA 241

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           RAFLYPV++  DLRGFE+L+V+HPTDEVINS+++AR
Sbjct: 242 RAFLYPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of Lsi11G006330 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 3.1e-132
Identity = 250/281 (88.97%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLV-LICTPPPSSPSSIDISTLCS 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLFSQLV LICTP   S SSID+STLCS
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTP---SSSSIDVSTLCS 60

Query: 61  TVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNH 120
           T+QTMRSHLIKLCGEAE+LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSILNH
Sbjct: 61  TIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNH 120

Query: 121 HAPHA---PPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDAD 180
           HAPH+   PPSKVAFVGSGALPFSSIIMALKY PK EFHNFD+D SANAKA+MLVAGD +
Sbjct: 121 HAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVE 180

Query: 181 LSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRS 240
           LSRRMVFHTTDIMDV S ELR+F+VVFLAALVGME+EEK KVIEHLRKNM GGALLMLRS
Sbjct: 181 LSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           AYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 AYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of Lsi11G006330 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.9e-131
Identity = 249/280 (88.93%), Postives = 263/280 (93.93%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLV-LICTPP--PSSPSSIDISTL 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLFSQLV LICTPP   SSPSSID+S+L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  CSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CS++QTMRS+LIKLCGEAE+LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADL 180
           NHH    PPSKVAFVGSGALPFSSIIMALKYLPK EFHNFD+D SANAKA+MLVAGD +L
Sbjct: 121 NHHIT-PPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVEL 180

Query: 181 SRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSA 240
           SRRMVFHTTDIM+V S ELR+F+VVFLAALVGME+EEK KVIEHLRKNM GGALLMLRSA
Sbjct: 181 SRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of Lsi11G006330 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 9.1e-124
Identity = 238/282 (84.40%), Postives = 251/282 (89.01%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSS---IDIS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVL CTPPP  P S   IDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  TLCSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L STVQTMRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDA 180
           IL  +   A PSKVAF+GSGALPFSSIIMALKYLP  EFHNFDVDSSANAKA MLVA D 
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLR 240
           DLSRRM FHTTDIMDV S+ LR+F+VVFLAALVGMEKEEKVKVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Lsi11G006330 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 8.5e-122
Identity = 235/282 (83.33%), Postives = 249/282 (88.30%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSS---IDIS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVL CTPPP  P S   IDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  TLCSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L STVQTMRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDA 180
           IL  +   + PSKVAF+GSGALPFSSIIMALKYLP  EFHNFDVDSSANAKA  LVA D 
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLR 240
           DLSRRM FHTTDIMDV S+ L +F+VVFLAALVGMEKEEKVKVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Lsi11G006330 vs. ExPASy TrEMBL
Match: A0A6J1DTD8 (probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 2.7e-120
Identity = 232/277 (83.75%), Postives = 247/277 (89.17%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           MCCQ +LLVQK+SELYHKISTL+SLKPSKDVNSLFSQLVL C PPP SP  ID+S L S 
Sbjct: 1   MCCQEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCAPPPPSPPPIDVSKLGSA 60

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120
           VQ MRSHLIKLCGEAEALLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFSIL   
Sbjct: 61  VQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFSILER- 120

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRR 180
             HA PSKVAFVGSGALPF+SIIMA KYLP  EFHNFDVD SANAKAA LVAGD DL RR
Sbjct: 121 --HAQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDRDLERR 180

Query: 181 MVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYGA 240
           MVFHT DIM+V + ELR FQVVFLAALVGME+EEKVKVIEHL KNMGGGALLMLRSAYGA
Sbjct: 181 MVFHTADIMEV-TGELRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLRSAYGA 240

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           RAFLYPVVEA DL+GFEILTVFHPTDEVINS+VLARL
Sbjct: 241 RAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of Lsi11G006330 vs. NCBI nr
Match: XP_038891237.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 519.2 bits (1336), Expect = 2.1e-143
Identity = 262/277 (94.58%), Postives = 273/277 (98.56%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLF+QLVLIC+PPPSSPSSID+STLCS 
Sbjct: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFTQLVLICSPPPSSPSSIDVSTLCSA 60

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120
           VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH
Sbjct: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNHH 120

Query: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRR 180
           APHAPPSKVAFVGSGALPFSSIIMALKYLPK E HNFD+D SANAKAAMLVAGDA+LS+R
Sbjct: 121 APHAPPSKVAFVGSGALPFSSIIMALKYLPKTELHNFDIDPSANAKAAMLVAGDAELSKR 180

Query: 181 MVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYGA 240
           MVFHTTDIMDVAS+ELR+F+VVFLAALVGME+EEKVKVIEHLRK+MGGGALLMLRSAYGA
Sbjct: 181 MVFHTTDIMDVASDELREFEVVFLAALVGMEREEKVKVIEHLRKSMGGGALLMLRSAYGA 240

Query: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 RAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 277

[Close]

BLAST of Lsi11G006330 vs. NCBI nr
Match: XP_004144812.1 (nicotianamine synthase 1 [Cucumis sativus])

HSP 1 Score: 481.1 bits (1237), Expect = 6.4e-132
Identity = 250/281 (88.97%), Postives = 263/281 (93.59%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLV-LICTPPPSSPSSIDISTLCS 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLFSQLV LICTP   S SSID+STLCS
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTP---SSSSIDVSTLCS 60

Query: 61  TVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILNH 120
           T+QTMRSHLIKLCGEAE+LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSILNH
Sbjct: 61  TIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSILNH 120

Query: 121 HAPHA---PPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDAD 180
           HAPH+   PPSKVAFVGSGALPFSSIIMALKY PK EFHNFD+D SANAKA+MLVAGD +
Sbjct: 121 HAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLVAGDVE 180

Query: 181 LSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRS 240
           LSRRMVFHTTDIMDV S ELR+F+VVFLAALVGME+EEK KVIEHLRKNM GGALLMLRS
Sbjct: 181 LSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGALLMLRS 240

Query: 241 AYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           AYGARAF+YPVVEACDLRGF+ILTVFHPTDEVINSIVLARL
Sbjct: 241 AYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of Lsi11G006330 vs. NCBI nr
Match: XP_008453494.1 (PREDICTED: nicotianamine synthase 1-like [Cucumis melo])

HSP 1 Score: 476.9 bits (1226), Expect = 1.2e-130
Identity = 249/280 (88.93%), Postives = 263/280 (93.93%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLV-LICTPP--PSSPSSIDISTL 60
           MCCQ LLLVQKISELYHKISTLESLKPSK VNSLFSQLV LICTPP   SSPSSID+S+L
Sbjct: 1   MCCQSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVSSL 60

Query: 61  CSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           CS++QTMRS+LIKLCGEAE+LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFSIL
Sbjct: 61  CSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFSIL 120

Query: 121 NHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADL 180
           NHH    PPSKVAFVGSGALPFSSIIMALKYLPK EFHNFD+D SANAKA+MLVAGD +L
Sbjct: 121 NHHIT-PPPSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDVEL 180

Query: 181 SRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSA 240
           SRRMVFHTTDIM+V S ELR+F+VVFLAALVGME+EEK KVIEHLRKNM GGALLMLRSA
Sbjct: 181 SRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL
Sbjct: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of Lsi11G006330 vs. NCBI nr
Match: XP_023538584.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454.5 bits (1168), Expect = 6.4e-124
Identity = 236/282 (83.69%), Postives = 252/282 (89.36%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSS---IDIS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVL CTPPP  P S   ID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDVS 60

Query: 61  TLCSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L STVQTMRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  NLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDA 180
           IL  +   +PPSKVAF+GSGALPFSSIIMALKYLP  EFHNFDVDSSANAKA  LVA D 
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAMALVASDV 180

Query: 181 DLSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLR 240
           DLS+RM+FHTTDIMDV SN LR+F+VVFLAALVGMEKEEKVKVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Lsi11G006330 vs. NCBI nr
Match: XP_022965879.1 (nicotianamine synthase [Cucurbita maxima])

HSP 1 Score: 453.0 bits (1164), Expect = 1.9e-123
Identity = 238/282 (84.40%), Postives = 251/282 (89.01%), Query Frame = 0


Query: 1   MCC--QGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSS---IDIS 60
           MCC  +GLLLVQK+SELYHKISTL+SL+PSK VNSLFSQLVL CTPPP  P S   IDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  TLCSTVQTMRSHLIKLCGEAEALLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L STVQTMRSHLIKLCG+AE LLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILNHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDA 180
           IL  +   A PSKVAF+GSGALPFSSIIMALKYLP  EFHNFDVDSSANAKA MLVA D 
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLR 240
           DLSRRM FHTTDIMDV S+ LR+F+VVFLAALVGMEKEEKVKVIEHLRKNM  G+LLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 278
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of Lsi11G006330 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 322.0 bits (824), Expect = 4.7e-88
Identity = 163/279 (58.42%), Postives = 219/279 (78.49%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           M CQ  L+V++I +LY +IS L+SLKPSK+V++LF QLV  C P   + ++ID++ +C  
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLP---TDTNIDVTNMCEE 60

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILAS---HQNPLDHLSLFPYYSNYLKLTHLEFSIL 120
           V+ MR++LIKLCGEAE  LE H+STIL S    QNPLDHL +FPYYSNYLKL  LEF +L
Sbjct: 61  VKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLL 120

Query: 121 NHHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADL 180
           + H+ H  P+K+AFVGSG +P +SI++A  +LP   FHNFD+DS AN  A+ LV+ D DL
Sbjct: 121 SQHSSHV-PTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDL 180

Query: 181 SRRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSA 240
           S+RM+FHTTD+++ A+  L Q+ VVFLAALVGM+KE KVK IEHL K+M  GA+LMLRSA
Sbjct: 181 SKRMIFHTTDVLN-ATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSA 240

Query: 241 YGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           +  RAFLYP+V++ DL+GF++LT++HPTD+V+NS+V+AR
Sbjct: 241 HALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of Lsi11G006330 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 321.2 bits (822), Expect = 7.9e-88
Identity = 167/275 (60.73%), Postives = 210/275 (76.36%), Query Frame = 0


Query: 3   CQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCSTVQ 62
           CQ   LV KI +LY KIS LE+LKP +DV++LF QLV  C PP     +ID++ +   +Q
Sbjct: 4   CQDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPP---NPNIDVTKMSENIQ 63

Query: 63  TMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNHHA 122
            MRS+LIK+CGEAE  LEHH+S+IL S + NPL HL+LFPYY+NYLKL+ LEF +L  + 
Sbjct: 64  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 123

Query: 123 PHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSRRM 182
               P  VAF+GSG LP +S+++A  +L  + FHNFD+D SAN  AA LV+ D DLS+RM
Sbjct: 124 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 183

Query: 183 VFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYGAR 242
            FHT DIMDV +  L+ F VVFLAALVGM+K+EKVKV+EHL K+M  GALLMLRSA+G R
Sbjct: 184 FFHTVDIMDV-TESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPR 243

Query: 243 AFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           AFLYP+VE CDL GFE+L+V+HPTDEVINSIV++R
Sbjct: 244 AFLYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of Lsi11G006330 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 165/277 (59.57%), Postives = 212/277 (76.53%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTLCST 60
           M CQ   LVQ I +LY KIS LESLKPS+DVN LF QLV  C PP     +ID++ +C  
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPP---NPNIDVTKMCDR 60

Query: 61  VQTMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILNH 120
           VQ +R +LIK+CG AE  LE+H+S+IL S+Q NPL HL++FPYY+NYLKL  LEF +L  
Sbjct: 61  VQEIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQ 120

Query: 121 HAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLSR 180
           +     P  VAF+GSG LP +SI++A  +L    FHNFD+D SAN+ A++LV+ D D+S+
Sbjct: 121 NLNGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQ 180

Query: 181 RMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAYG 240
           RM FHT DIMDV +  L+ F VVFLAALVGM KEEKVKVIEHL+K+M  GA+LMLRSA+G
Sbjct: 181 RMFFHTVDIMDV-TESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHG 240

Query: 241 ARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
            RAFLYP+VE CDL+GFE+L+++HPTD+VINS+V+++
Sbjct: 241 PRAFLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of Lsi11G006330 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 317.4 bits (812), Expect = 1.1e-86
Identity = 161/278 (57.91%), Postives = 219/278 (78.78%), Query Frame = 0


Query: 1   MCCQGLLLVQKISELYHKISTLESLKPSKDVNSLFSQLVLICTPPPSSPSSIDISTL-CS 60
           M C+  L+V++I +LY++IS LESLKPSK+V++LF QLV  C P   + ++ID++ +   
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLP---TDTNIDVTEIHDE 60

Query: 61  TVQTMRSHLIKLCGEAEALLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILN 120
            V+ MRSHLIKLCGEAE  LE H+S IL S + NPL+HL +FPYY+NYLKL  LEF +L+
Sbjct: 61  KVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLS 120

Query: 121 HHAPHAPPSKVAFVGSGALPFSSIIMALKYLPKAEFHNFDVDSSANAKAAMLVAGDADLS 180
            H  H  P+KVAF+GSG +P +SI++A  +LP   FHNFD+DS AN  A+ LV+ D+DLS
Sbjct: 121 QHTTHV-PTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLS 180

Query: 181 RRMVFHTTDIMDVASNELRQFQVVFLAALVGMEKEEKVKVIEHLRKNMGGGALLMLRSAY 240
           +RM+FHTTD+++ A   L Q+ VVFLAALVGM+KE KVK IEHL K+M  GA++MLRSA+
Sbjct: 181 KRMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAH 240

Query: 241 GARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLAR 277
           G RAFLYP+V++CDL+GFE+LT++HP+D+V+NS+V+AR
Sbjct: 241 GLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of Lsi11G006330 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 63.2 bits (152), Expect = 3.9e-10
Identity = 30/53 (56.60%), Postives = 41/53 (77.36%), Query Frame = 0


Query: 226 MGGGALLMLRSAYGARAFLYPVVEACDLRGFEILTVFHP--TDEVINSIVLAR 277
           M  GALLMLRSA G R+FLY  V+ CDL+GFE+L ++HP  +D  +NS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of Lsi11G006330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FF79	6.6e-87	58.42	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9C7X5	1.1e-86	60.73	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
Q9FKT9	1.6e-85	57.91	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]
O80483	1.6e-85	59.57	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9XGI7	5.2e-84	57.25	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]

BLAST of Lsi11G006330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJ09	3.1e-132	88.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A1S3BXJ6	5.9e-131	88.93	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A6J1HS54	9.1e-124	84.40	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A6J1FHL8	8.5e-122	83.33	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]
A0A6J1DTD8	2.7e-120	83.75	probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309...	[more]

BLAST of Lsi11G006330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891237.1	2.1e-143	94.58	probable nicotianamine synthase 4 [Benincasa hispida]	[more]
XP_004144812.1	6.4e-132	88.97	nicotianamine synthase 1 [Cucumis sativus]	[more]
XP_008453494.1	1.2e-130	88.93	PREDICTED: nicotianamine synthase 1-like [Cucumis melo]	[more]
XP_023538584.1	6.4e-124	83.69	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022965879.1	1.9e-123	84.40	nicotianamine synthase [Cucurbita maxima]	[more]

BLAST of Lsi11G006330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04950.1	4.7e-88	58.42	nicotianamine synthase 1	[more]
AT1G56430.1	7.9e-88	60.73	nicotianamine synthase 4	[more]
AT1G09240.1	1.1e-86	59.57	nicotianamine synthase 3	[more]
AT5G56080.1	1.1e-86	57.91	nicotianamine synthase 2	[more]
AT4G26483.1	3.9e-10	56.60	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 4..277 e-value: 4.0E-61 score: 208.5
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 1..276
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 7..276 e-value: 9.4E-112 score: 372.8
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 1..276
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 1..277 score: 52.595741
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 100..237

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G006330.1	Lsi11G006330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity