Lsi11G006010 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G006010
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGDSL esterase/lipase
Locationchr11: 6361909 .. 6363365 (-)
RNA-Seq ExpressionLsi11G006010
SyntenyLsi11G006010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCGACGCTATTTACCAACTTGGTGACTCAATCTCCGACACTGGAAATCTCATTCGTGAAAATCCCAACACTCCATTTTCTCATCTTCCTTATGGACAATCTTTCTTCAACAATCCCACTGGTCGATGCTCCAACGGTCTACTTATGCTCGATTACTTTGGTAACCATTTTTTTAAACCTTATATGTTCTTATTTTTCTTTCATTAATTCGTTCTATAGCTCCATGATGATGCATATCTAATAACAATATATAATGGTTACTTCTATTTGCATGCAGCTTTAGATGCCGGACTTCCTTTCGTGAGTCCTTATTTAAATAAAGATGCCTTGATGGATCATGGAGTCAACTTTGCGGTGGCTGGTTCTACAGCTTTGCCTTCTCAATATCTTTCTACGAAGAAAATTTTATCTCCAGTTACTAACTCTTCTCTTGACCATCAACTTGATTGGATGTTCTCTCATTTCAATTCCATTTGTCGCAATCAAAGAGGTAATTAATTAACCTTCTACTTTTAATTTTATACGTATACTCACATTTTTTTCCAATATTTTTCTACATTCTACATTTGATGTTTGACAATTTCATACATATTTAATTACTATATAGATTGCAACGAGAAATTAGGGAATTCTTTGTTCTTGGTGGGTGAGATTGGTGGCAATGACTATAACTATGCACTATTCCAAGGAAAAACTATTCAAGAGGCAAAAGATATGGTGCCTGATGTTGTTCGAACCATAAAGAGTGTTGTTGAGGTAATAATTTTTGCCTAGAATAGATCATATTCCATATTTTTTTGTTACTGATCATATGAATGAGAGGTTTATTTACTTACTTTTCTTTTGTGTGTAATGTTTGTAGAAAGTAATAAGCTATGGTGCCACTCGAGTTATTGTTCCTGGAAATTTTCCTATTGGATGTTTCCCCATCTATCTCACTGGATTCCAAACCAATGATACATCTGCTTATGATGAACTTCATTGTTTAAAAGATTTGAACAGTTTTGCTAGCTATCACAACCATAAAATCAAGCAAGTCATTGAAGTACTCAAGAAGGAGAATCCACATACTGTTATTGTATATGGTGACTATTACAATGCATTCCTATGGATTCTTTGTCATGCTTTCGTACTAGGTAATTTTTCCCTCCCTACAAAATAATATTATATCTTAATTTAAAAAATAATTTCTCTCAAATCTTTAATGCCTCTACCTACTTTATCATACATGAACAGGATTTGATGAAGGTTCTTTGCAAAAATCATGTTGTGGGATTGGAGGAAATTACAACTTTAACCTCATGCAAATGTGTGGACTTCCTGGAGTGCCCGTTTGTCCAAACCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAAAAGGCTTACAAATTTATGGCACATTGGCTCATCCATGACATCTTCCCAAAATTACATTGCATTGTTTAA

mRNA sequence

ATGTTCGACGCTATTTACCAACTTGGTGACTCAATCTCCGACACTGGAAATCTCATTCGTGAAAATCCCAACACTCCATTTTCTCATCTTCCTTATGGACAATCTTTCTTCAACAATCCCACTGGTCGATGCTCCAACGGTCTACTTATGCTCGATTACTTTGCTTTAGATGCCGGACTTCCTTTCGTGAGTCCTTATTTAAATAAAGATGCCTTGATGGATCATGGAGTCAACTTTGCGGTGGCTGGTTCTACAGCTTTGCCTTCTCAATATCTTTCTACGAAGAAAATTTTATCTCCAGTTACTAACTCTTCTCTTGACCATCAACTTGATTGGATGTTCTCTCATTTCAATTCCATTTGTCGCAATCAAAGAGATTGCAACGAGAAATTAGGGAATTCTTTGTTCTTGGTGGGTGAGATTGGTGGCAATGACTATAACTATGCACTATTCCAAGGAAAAACTATTCAAGAGGCAAAAGATATGGTGCCTGATGTTGTTCGAACCATAAAGAGTGTTGTTGAGAAAGTAATAAGCTATGGTGCCACTCGAGTTATTGTTCCTGGAAATTTTCCTATTGGATGTTTCCCCATCTATCTCACTGGATTCCAAACCAATGATACATCTGCTTATGATGAACTTCATTGTTTAAAAGATTTGAACAGTTTTGCTAGCTATCACAACCATAAAATCAAGCAAGTCATTGAAGTACTCAAGAAGGAGAATCCACATACTGTTATTGTATATGGTGACTATTACAATGCATTCCTATGGATTCTTTGTCATGCTTTCGTACTAGGATTTGATGAAGGTTCTTTGCAAAAATCATGTTGTGGGATTGGAGGAAATTACAACTTTAACCTCATGCAAATGTGTGGACTTCCTGGAGTGCCCGTTTGTCCAAACCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAAAAGGCTTACAAATTTATGGCACATTGGCTCATCCATGACATCTTCCCAAAATTACATTGCATTGTTTAA

Coding sequence (CDS)

ATGTTCGACGCTATTTACCAACTTGGTGACTCAATCTCCGACACTGGAAATCTCATTCGTGAAAATCCCAACACTCCATTTTCTCATCTTCCTTATGGACAATCTTTCTTCAACAATCCCACTGGTCGATGCTCCAACGGTCTACTTATGCTCGATTACTTTGCTTTAGATGCCGGACTTCCTTTCGTGAGTCCTTATTTAAATAAAGATGCCTTGATGGATCATGGAGTCAACTTTGCGGTGGCTGGTTCTACAGCTTTGCCTTCTCAATATCTTTCTACGAAGAAAATTTTATCTCCAGTTACTAACTCTTCTCTTGACCATCAACTTGATTGGATGTTCTCTCATTTCAATTCCATTTGTCGCAATCAAAGAGATTGCAACGAGAAATTAGGGAATTCTTTGTTCTTGGTGGGTGAGATTGGTGGCAATGACTATAACTATGCACTATTCCAAGGAAAAACTATTCAAGAGGCAAAAGATATGGTGCCTGATGTTGTTCGAACCATAAAGAGTGTTGTTGAGAAAGTAATAAGCTATGGTGCCACTCGAGTTATTGTTCCTGGAAATTTTCCTATTGGATGTTTCCCCATCTATCTCACTGGATTCCAAACCAATGATACATCTGCTTATGATGAACTTCATTGTTTAAAAGATTTGAACAGTTTTGCTAGCTATCACAACCATAAAATCAAGCAAGTCATTGAAGTACTCAAGAAGGAGAATCCACATACTGTTATTGTATATGGTGACTATTACAATGCATTCCTATGGATTCTTTGTCATGCTTTCGTACTAGGATTTGATGAAGGTTCTTTGCAAAAATCATGTTGTGGGATTGGAGGAAATTACAACTTTAACCTCATGCAAATGTGTGGACTTCCTGGAGTGCCCGTTTGTCCAAACCCTAATGAACATATCAGTTGGGATGGAGTCCATTTGACACAAAAGGCTTACAAATTTATGGCACATTGGCTCATCCATGACATCTTCCCAAAATTACATTGCATTGTTTAA

Protein sequence

MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGLPFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHFNSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPVCPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV
Homology
BLAST of Lsi11G006010 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 1.6e-82
Identity = 150/338 (44.38%), Postives = 208/338 (61.54%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLIRENPNTP---FSHLPYGQSFFNNPTGRCSNGLLMLDYFALDA 61
           FD+IYQLGDS SDTGNLIR  P+ P    +H PYG++F   PTGRCS+G L++D+ A   
Sbjct: 27  FDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGETFPGTPTGRCSDGRLIIDFIATAL 86

Query: 62  GLPFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHFN 121
            LP ++PYL ++    HGVNFAVAG+TAL   +L+ + +     +S L  QL+W  ++  
Sbjct: 87  NLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAARGVQVSDIHSHLSAQLNWFRTYLG 146

Query: 122 SICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVI 181
           SIC   ++C+ KL N+LF++G IG ND NYA F  +TI+E +  VP +   + +   ++I
Sbjct: 147 SICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNRTIEEIRAYVPFITEAVANATREII 206

Query: 182 SYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVL 241
             G +RVIVPG FPIGC    L           D+L CL  LN+ + Y N   ++ +  L
Sbjct: 207 RLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDLGCLSSLNNLSIYFNSLFQRALASL 266

Query: 242 KKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVP 301
             E P  VI+Y DYYNA+ ++  +   LG +  SL K CCGIGG YN++  + CG  GVP
Sbjct: 267 SIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLLKCCCGIGGPYNYDPDRECGSRGVP 326

Query: 302 VCPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHC 337
           VCPNP ++I WDG H TQ AY+ +A ++I  I   L C
Sbjct: 327 VCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKALKC 363

BLAST of Lsi11G006010 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.6e-77
Identity = 146/334 (43.71%), Postives = 210/334 (62.87%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLI-----RENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFAL 61
           F +I   GDSI+DTGNL+      + P T F   PYG++FF++PTGR  +G +++D+ A 
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGETFFHHPTGRSCDGRIIMDFIAE 88

Query: 62  DAGLPFVSPYL-NKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFS 121
             GLP+V PY  +K+   D GVNFAVAG+TAL S +L  K+ + P TN SL  QL     
Sbjct: 89  FVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLK-KRGIQPHTNVSLGVQLKSFKK 148

Query: 122 HFNSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVE 181
              ++C +  DC + +GN+L L+GEIGGNDYN+  F  K ++E +++VP V+ +I S + 
Sbjct: 149 SLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTIT 208

Query: 182 KVISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYD-ELHCLKDLNSFASYHNHKIKQV 241
           ++I  G    +VPG FPIGC  +YLT ++T++   YD    CLK LN F  YH+ K+K  
Sbjct: 209 ELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVE 268

Query: 242 IEVLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGL 301
           +  L+K  PH  I+Y DYYN+ L I       GF E     +CCGIGG YNFN  + CG 
Sbjct: 269 LNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCGS 328

Query: 302 PGVPVCPNPNEHISWDGVHLTQKAYKFMAHWLIH 329
            GV  C +P++++ WDGVH+T+ AYK++A  +++
Sbjct: 329 VGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

BLAST of Lsi11G006010 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 7.0e-75
Identity = 151/335 (45.07%), Postives = 192/335 (57.31%), Query Frame = 0

Query: 3   DAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGLPF 62
           ++IYQ GDSISDTGNLIR  P +  +  P  Q   N                        
Sbjct: 28  NSIYQFGDSISDTGNLIRNGPASSPTPKPLPQREHN------------------------ 87

Query: 63  VSPYLNKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHFNSICR 122
                         VNF V+GSTAL S + S + +  P TN+ L  QL W   H  S C 
Sbjct: 88  ------------VFVNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCH 147

Query: 123 -NQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVISYG 182
            +  DC   L +SLF+VGEIGGNDYNY  FQGK ++E +  +P VV  I +   +VI  G
Sbjct: 148 GSSSDC---LKHSLFMVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAG 207

Query: 183 ATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLKKE 242
           A  V+VPGNFP+GCFPIYLT F   DT  YD+  CL  LN FA  HN+++++ I  L+KE
Sbjct: 208 AVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKE 267

Query: 243 NPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPVCP 302
            P   IVYGDYYNAF ++L       FD+    KSCCG GG YN++  +  G  GVPVC 
Sbjct: 268 FPDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQ 320

Query: 303 NPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHC 337
           NP++ ISWDGVHLTQKAY+FM+ +L + I  ++ C
Sbjct: 328 NPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

BLAST of Lsi11G006010 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 282.0 bits (720), Expect = 9.2e-75
Identity = 142/331 (42.90%), Postives = 212/331 (64.05%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLI-RENPN-TPFSHL-PYGQSFFNNPTGRCSNGLLMLDYFALDA 61
           F +I   GDSI+DTGNL+   +PN  P S   PYG++FF++PTGR S+G L++D+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  GLPFVSPYLN-KDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHF 121
           G P V P+   ++A    GVNFAVAG+TAL   +L  + I S +TN SL  QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 122 NSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKV 181
            ++C +  DC + + N+L L+GEIGGNDYN+ALFQ K ++E +++VP V+ TI S + ++
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 182 ISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDEL-HCLKDLNSFASYHNHKIKQVIE 241
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK LN F+ Y+N ++++ + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 242 VLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPG 301
            L+K  PH  I+Y DYYNA L +       GF    L  +CCG+GG+YNFN  + CG  G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 302 VPVCPNPNEHISWDGVHLTQKAYKFMAHWLI 328
           V  C +P++++++DG+H+T+ AY+ ++  L+
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of Lsi11G006010 vs. ExPASy Swiss-Prot
Match: Q9SHP6 (GDSL esterase/lipase At1g28610 OS=Arabidopsis thaliana OX=3702 GN=At1g28610 PE=2 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 2.0e-74
Identity = 143/332 (43.07%), Postives = 207/332 (62.35%), Query Frame = 0

Query: 3   DAIYQLGDSISDTGNLI-----RENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALD 62
           ++I   GDSI+DTGNL+        P T F  LPYG++FF++PTGR  NG +++D+ A  
Sbjct: 30  ESIISFGDSITDTGNLVGLSDRNHLPVTAF--LPYGETFFHHPTGRSCNGRIIIDFIAEF 89

Query: 63  AGLPFVSP-YLNKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSH 122
            GLP V P Y +K+   + GVNFAVAG+TAL +  L  + I  P +N SL  QL      
Sbjct: 90  LGLPHVPPFYGSKNGNFEKGVNFAVAGATALETSILEKRGIYYPHSNISLGIQLKTFKES 149

Query: 123 FNSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEK 182
             ++C +  DC + +GN+  ++GEIGGND+N+A F  KT  E K++VP V+  I S + +
Sbjct: 150 LPNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKT-SEVKELVPLVITKISSAIVE 209

Query: 183 VISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDEL-HCLKDLNSFASYHNHKIKQVI 242
           ++  G    +VPGNFP+GC   YLT +QT++   YD L  CL  LN F+ Y+N K++  +
Sbjct: 210 LVDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAEL 269

Query: 243 EVLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLP 302
             L K  PH  I+YGDY+NA L +       GF +  L  +CCG+GG YNF L + CG  
Sbjct: 270 NRLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPL-PACCGLGGPYNFTLSKKCGSV 329

Query: 303 GVPVCPNPNEHISWDGVHLTQKAYKFMAHWLI 328
           GV  C +P+++++WDGVH+T+ AYK++A  L+
Sbjct: 330 GVKYCSDPSKYVNWDGVHMTEAAYKWIADGLL 357

BLAST of Lsi11G006010 vs. ExPASy TrEMBL
Match: A0A1S4E629 (acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.3e-185
Identity = 308/339 (90.86%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTAL SQYLSTK KILSPVTNSSLDHQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LF VGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN FA+YHN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HA VLGFDE SLQKSCCGIGG+YNFNLMQMCG+ GV  
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. ExPASy TrEMBL
Match: A0A5D3CNR6 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold745G00130 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 8.6e-185
Identity = 307/339 (90.56%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTAL SQYLSTK KILSPVTNSSLDHQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LF VGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN FA++HN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATHHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HA VLGFDE SLQKSCCGIGG+YNFNLMQMCG+ GV  
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. ExPASy TrEMBL
Match: A0A5A7SSW2 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold219G001390 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.9e-184
Identity = 307/339 (90.56%), Postives = 318/339 (93.81%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTAL SQYLSTK KILSPVTNSSLDHQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LF VGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN FA+YHN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HA VLGFDE SLQKSCCGIGG+YNFNLMQMCG+ GV  
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWD VHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDRVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. ExPASy TrEMBL
Match: A0A5A7TWR0 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001010 PE=3 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 2.5e-184
Identity = 306/339 (90.27%), Postives = 318/339 (93.81%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 60

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD  MDHGVNFAVAGSTALPSQYL T  KILSPVTNSSLDHQLDWMFSHFNS
Sbjct: 61  PLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSLDHQLDWMFSHFNS 120

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LFLVGEIGGNDYNYALFQGKTI+EAKDMVPDVV+TIKS VEKVIS
Sbjct: 121 ICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPDVVQTIKSAVEKVIS 180

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN  A+Y+N++IKQ IEVLK
Sbjct: 181 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLATYYNNQIKQTIEVLK 240

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HAFVLGFDEGSLQKSCCGIGG Y FNLMQMCG+ GV V
Sbjct: 241 KENPHTVIVYGDYYNAFLWIIRHAFVLGFDEGSLQKSCCGIGGEYRFNLMQMCGVEGVEV 300

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQ  YKFM HWLIHDIFPKLHCIV
Sbjct: 301 CPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

BLAST of Lsi11G006010 vs. ExPASy TrEMBL
Match: A0A5A7VJA5 (Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold488G00480 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 3.6e-183
Identity = 305/339 (89.97%), Postives = 316/339 (93.22%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 60

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
             VSPYLNKD  MDHGVNFAVAGSTALPSQYL T  KILSPVTNSSLDHQLDWMFSHFNS
Sbjct: 61  SLVSPYLNKDGSMDHGVNFAVAGSTALPSQYLFTNYKILSPVTNSSLDHQLDWMFSHFNS 120

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LFLVGEIGGNDYNYALFQGKTI+EAKDMVPDVV+TIKS VEKVIS
Sbjct: 121 ICHNQRDCNEKLRSALFLVGEIGGNDYNYALFQGKTIEEAKDMVPDVVQTIKSAVEKVIS 180

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN  A+YHN++IKQ IEVLK
Sbjct: 181 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGLATYHNNQIKQTIEVLK 240

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HAFVLGFDE SLQKSCCGIGG Y FNLMQMCG+ GV V
Sbjct: 241 KENPHTVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGEYRFNLMQMCGVEGVEV 300

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQ  YKFM HWLIHDIFPKLHCIV
Sbjct: 301 CPNPNEHISWDGVHLTQSTYKFMTHWLIHDIFPKLHCIV 339

BLAST of Lsi11G006010 vs. NCBI nr
Match: XP_016903430.1 (PREDICTED: acetylajmalan esterase-like [Cucumis melo])

HSP 1 Score: 657.9 bits (1696), Expect = 4.7e-185
Identity = 308/339 (90.86%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTAL SQYLSTK KILSPVTNSSLDHQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LF VGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN FA+YHN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HA VLGFDE SLQKSCCGIGG+YNFNLMQMCG+ GV  
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. NCBI nr
Match: TYK13517.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 656.0 bits (1691), Expect = 1.8e-184
Identity = 307/339 (90.56%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTAL SQYLSTK KILSPVTNSSLDHQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LF VGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN FA++HN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATHHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HA VLGFDE SLQKSCCGIGG+YNFNLMQMCG+ GV  
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. NCBI nr
Match: XP_011659746.1 (acetylajmalan esterase-like [Cucumis sativus])

HSP 1 Score: 655.2 bits (1689), Expect = 3.0e-184
Identity = 306/339 (90.27%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+ ALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTALPSQYLS+  KI+SPVTNSSLDHQLDWMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQR+CNEKL ++LFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLNSFA+YHN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPH VIVYGDYYNAFLWI+ HAFVLGFDE SLQKSCCGIGG+Y FNLMQMCG+ GV  
Sbjct: 272 KENPHAVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. NCBI nr
Match: KAE8646130.1 (hypothetical protein Csa_016115 [Cucumis sativus])

HSP 1 Score: 655.2 bits (1689), Expect = 3.0e-184
Identity = 306/339 (90.27%), Postives = 319/339 (94.10%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+ ALDAGL
Sbjct: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFLALDAGL 60

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTALPSQYLS+  KI+SPVTNSSLDHQLDWMFSHFNS
Sbjct: 61  PLVSPYLNKDGLMDHGVNFAVAGSTALPSQYLSSSYKIISPVTNSSLDHQLDWMFSHFNS 120

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQR+CNEKL ++LFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 121 ICHNQRECNEKLRSALFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 180

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLNSFA+YHN +IKQ IEVLK
Sbjct: 181 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNSFATYHNDQIKQAIEVLK 240

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPH VIVYGDYYNAFLWI+ HAFVLGFDE SLQKSCCGIGG+Y FNLMQMCG+ GV  
Sbjct: 241 KENPHAVIVYGDYYNAFLWIIRHAFVLGFDEESLQKSCCGIGGDYKFNLMQMCGVAGVEA 300

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWDGVHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 301 CPNPNEHISWDGVHLTQKTYKFMTHWLIHDIFPKLHCIV 339

BLAST of Lsi11G006010 vs. NCBI nr
Match: KAA0032295.1 (acetylajmalan esterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 654.8 bits (1688), Expect = 4.0e-184
Identity = 307/339 (90.56%), Postives = 318/339 (93.81%), Query Frame = 0

Query: 1   MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGL 60
           MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLD+FALDAGL
Sbjct: 32  MFDAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDFFALDAGL 91

Query: 61  PFVSPYLNKDALMDHGVNFAVAGSTALPSQYLSTK-KILSPVTNSSLDHQLDWMFSHFNS 120
           P VSPYLNKD LMDHGVNFAVAGSTAL SQYLSTK KILSPVTNSSLDHQL+WMFSHFNS
Sbjct: 92  PLVSPYLNKDGLMDHGVNFAVAGSTALSSQYLSTKYKILSPVTNSSLDHQLEWMFSHFNS 151

Query: 121 ICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVIS 180
           IC NQRDCNEKL ++LF VGEIGGNDYNYALFQGKTIQEAKDMVPDVV+TIKS VEKVIS
Sbjct: 152 ICHNQRDCNEKLRSALFFVGEIGGNDYNYALFQGKTIQEAKDMVPDVVQTIKSAVEKVIS 211

Query: 181 YGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLK 240
           YGATRV+VPGNFPIGCFPIYLTGF TNDTSAYDELHCLKDLN FA+YHN +IKQ IEVLK
Sbjct: 212 YGATRVVVPGNFPIGCFPIYLTGFHTNDTSAYDELHCLKDLNGFATYHNDQIKQTIEVLK 271

Query: 241 KENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPV 300
           KENPHTVIVYGDYYNAFLWI+ HA VLGFDE SLQKSCCGIGG+YNFNLMQMCG+ GV  
Sbjct: 272 KENPHTVIVYGDYYNAFLWIIRHALVLGFDEDSLQKSCCGIGGDYNFNLMQMCGVAGVEA 331

Query: 301 CPNPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHCIV 339
           CPNPNEHISWD VHLTQK YKFM HWLIHDIFPKLHCIV
Sbjct: 332 CPNPNEHISWDRVHLTQKTYKFMTHWLIHDIFPKLHCIV 370

BLAST of Lsi11G006010 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 146/334 (43.71%), Postives = 210/334 (62.87%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLI-----RENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFAL 61
           F +I   GDSI+DTGNL+      + P T F   PYG++FF++PTGR  +G +++D+ A 
Sbjct: 29  FKSIISFGDSIADTGNLVGLSDRNQLPVTAFP--PYGETFFHHPTGRSCDGRIIMDFIAE 88

Query: 62  DAGLPFVSPYL-NKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFS 121
             GLP+V PY  +K+   D GVNFAVAG+TAL S +L  K+ + P TN SL  QL     
Sbjct: 89  FVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKSSFLK-KRGIQPHTNVSLGVQLKSFKK 148

Query: 122 HFNSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVE 181
              ++C +  DC + +GN+L L+GEIGGNDYN+  F  K ++E +++VP V+ +I S + 
Sbjct: 149 SLPNLCGSPSDCRDMIGNALILMGEIGGNDYNFPFFNRKPVKEVEELVPFVIASISSTIT 208

Query: 182 KVISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYD-ELHCLKDLNSFASYHNHKIKQV 241
           ++I  G    +VPG FPIGC  +YLT ++T++   YD    CLK LN F  YH+ K+K  
Sbjct: 209 ELIGMGGKTFLVPGEFPIGCSVVYLTLYKTSNKDEYDPSTGCLKWLNKFGEYHSEKLKVE 268

Query: 242 IEVLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGL 301
           +  L+K  PH  I+Y DYYN+ L I       GF E     +CCGIGG YNFN  + CG 
Sbjct: 269 LNRLRKLYPHVNIIYADYYNSLLRIFKEPAKFGFMERPF-PACCGIGGPYNFNFTRKCGS 328

Query: 302 PGVPVCPNPNEHISWDGVHLTQKAYKFMAHWLIH 329
            GV  C +P++++ WDGVH+T+ AYK++A  +++
Sbjct: 329 VGVKSCKDPSKYVGWDGVHMTEAAYKWIADGILN 358

BLAST of Lsi11G006010 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 282.3 bits (721), Expect = 5.0e-76
Identity = 151/335 (45.07%), Postives = 192/335 (57.31%), Query Frame = 0

Query: 3   DAIYQLGDSISDTGNLIRENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALDAGLPF 62
           ++IYQ GDSISDTGNLIR  P +  +  P  Q   N                        
Sbjct: 28  NSIYQFGDSISDTGNLIRNGPASSPTPKPLPQREHN------------------------ 87

Query: 63  VSPYLNKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHFNSICR 122
                         VNF V+GSTAL S + S + +  P TN+ L  QL W   H  S C 
Sbjct: 88  ------------VFVNFGVSGSTALNSSFFSERNLHVPATNTPLSMQLAWFKGHLRSTCH 147

Query: 123 -NQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKVISYG 182
            +  DC   L +SLF+VGEIGGNDYNY  FQGK ++E +  +P VV  I +   +VI  G
Sbjct: 148 GSSSDC---LKHSLFMVGEIGGNDYNYGFFQGKPMEEIRSYIPHVVGAITAAAREVIRAG 207

Query: 183 ATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDELHCLKDLNSFASYHNHKIKQVIEVLKKE 242
           A  V+VPGNFP+GCFPIYLT F   DT  YD+  CL  LN FA  HN+++++ I  L+KE
Sbjct: 208 AVNVVVPGNFPVGCFPIYLTSFPVKDTKDYDDNGCLTHLNEFAMDHNNQLQEAIASLRKE 267

Query: 243 NPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPGVPVCP 302
            P   IVYGDYYNAF ++L       FD+    KSCCG GG YN++  +  G  GVPVC 
Sbjct: 268 FPDVAIVYGDYYNAFQYVLRSE---RFDKSVALKSCCGTGGAYNYDGKRPYGAVGVPVCQ 320

Query: 303 NPNEHISWDGVHLTQKAYKFMAHWLIHDIFPKLHC 337
           NP++ ISWDGVHLTQKAY+FM+ +L + I  ++ C
Sbjct: 328 NPHKFISWDGVHLTQKAYRFMSKFLNNQILSQIKC 320

BLAST of Lsi11G006010 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 6.5e-76
Identity = 142/331 (42.90%), Postives = 212/331 (64.05%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNLI-RENPN-TPFSHL-PYGQSFFNNPTGRCSNGLLMLDYFALDA 61
           F +I   GDSI+DTGNL+   +PN  P S   PYG++FF++PTGR S+G L++D+ A   
Sbjct: 34  FKSIISFGDSIADTGNLLGLSDPNDLPASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFL 93

Query: 62  GLPFVSPYLN-KDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHF 121
           G P V P+   ++A    GVNFAVAG+TAL   +L  + I S +TN SL  QL       
Sbjct: 94  GFPLVPPFYGCQNANFKKGVNFAVAGATALEPSFLEERGIHSTITNVSLSVQLRSFTESL 153

Query: 122 NSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKV 181
            ++C +  DC + + N+L L+GEIGGNDYN+ALFQ K ++E +++VP V+ TI S + ++
Sbjct: 154 PNLCGSPSDCRDMIENALILMGEIGGNDYNFALFQRKPVKEVEELVPFVIATISSAITEL 213

Query: 182 ISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDEL-HCLKDLNSFASYHNHKIKQVIE 241
           +  G    +VPGNFPIG    YLT ++T++   YD L  CLK LN F+ Y+N ++++ + 
Sbjct: 214 VCMGGRTFLVPGNFPIGYSASYLTLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELN 273

Query: 242 VLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPG 301
            L+K  PH  I+Y DYYNA L +       GF    L  +CCG+GG+YNFN  + CG  G
Sbjct: 274 GLRKLYPHVNIIYADYYNALLRLFQEPAKFGFMNRPL-PACCGVGGSYNFNFSRRCGSVG 333

Query: 302 VPVCPNPNEHISWDGVHLTQKAYKFMAHWLI 328
           V  C +P++++++DG+H+T+ AY+ ++  L+
Sbjct: 334 VEYCDDPSQYVNYDGIHMTEAAYRLISEGLL 363

BLAST of Lsi11G006010 vs. TAIR 10
Match: AT1G28610.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-75
Identity = 143/332 (43.07%), Postives = 207/332 (62.35%), Query Frame = 0

Query: 3   DAIYQLGDSISDTGNLI-----RENPNTPFSHLPYGQSFFNNPTGRCSNGLLMLDYFALD 62
           ++I   GDSI+DTGNL+        P T F  LPYG++FF++PTGR  NG +++D+ A  
Sbjct: 30  ESIISFGDSITDTGNLVGLSDRNHLPVTAF--LPYGETFFHHPTGRSCNGRIIIDFIAEF 89

Query: 63  AGLPFVSP-YLNKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSH 122
            GLP V P Y +K+   + GVNFAVAG+TAL +  L  + I  P +N SL  QL      
Sbjct: 90  LGLPHVPPFYGSKNGNFEKGVNFAVAGATALETSILEKRGIYYPHSNISLGIQLKTFKES 149

Query: 123 FNSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEK 182
             ++C +  DC + +GN+  ++GEIGGND+N+A F  KT  E K++VP V+  I S + +
Sbjct: 150 LPNLCGSPTDCRDMIGNAFIIMGEIGGNDFNFAFFVNKT-SEVKELVPLVITKISSAIVE 209

Query: 183 VISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDEL-HCLKDLNSFASYHNHKIKQVI 242
           ++  G    +VPGNFP+GC   YLT +QT++   YD L  CL  LN F+ Y+N K++  +
Sbjct: 210 LVDMGGRTFLVPGNFPLGCSATYLTLYQTSNKEEYDPLTGCLTWLNDFSEYYNEKLQAEL 269

Query: 243 EVLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLP 302
             L K  PH  I+YGDY+NA L +       GF +  L  +CCG+GG YNF L + CG  
Sbjct: 270 NRLSKLYPHVNIIYGDYFNALLRLYQEPSKFGFMDRPL-PACCGLGGPYNFTLSKKCGSV 329

Query: 303 GVPVCPNPNEHISWDGVHLTQKAYKFMAHWLI 328
           GV  C +P+++++WDGVH+T+ AYK++A  L+
Sbjct: 330 GVKYCSDPSKYVNWDGVHMTEAAYKWIADGLL 357

BLAST of Lsi11G006010 vs. TAIR 10
Match: AT1G31550.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 1.5e-75
Identity = 144/331 (43.50%), Postives = 207/331 (62.54%), Query Frame = 0

Query: 2   FDAIYQLGDSISDTGNL--IRENPNTPFSHL-PYGQSFFNNPTGRCSNGLLMLDYFALDA 61
           F++I   GDSI+DTGNL  + ++ N P S   PYG++FF++PTGR S+G L++D+ A   
Sbjct: 34  FESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGETFFHHPTGRFSDGRLIIDFIAEFL 93

Query: 62  GLPFVSPYL-NKDALMDHGVNFAVAGSTALPSQYLSTKKILSPVTNSSLDHQLDWMFSHF 121
           GLP+V PY  + +   + GVNFAVA +TAL S +L  K    P  N SL  QL       
Sbjct: 94  GLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEEKGYHCP-HNFSLGVQLKIFKQSL 153

Query: 122 NSICRNQRDCNEKLGNSLFLVGEIGGNDYNYALFQGKTIQEAKDMVPDVVRTIKSVVEKV 181
            ++C    DC + +GN+L L+GEIG NDYN+  FQ + + E K++VP V+ TI S + ++
Sbjct: 154 PNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLRPLDEVKELVPLVISTISSAITEL 213

Query: 182 ISYGATRVIVPGNFPIGCFPIYLTGFQTNDTSAYDEL-HCLKDLNSFASYHNHKIKQVIE 241
           I  G    +VPG FP+GC   +LT  QT++   YD L  CLK LN F  YH+ ++++ + 
Sbjct: 214 IGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPLTGCLKWLNKFGEYHSEQLQEELN 273

Query: 242 VLKKENPHTVIVYGDYYNAFLWILCHAFVLGFDEGSLQKSCCGIGGNYNFNLMQMCGLPG 301
            L+K NPH  I+Y DYYNA L +       GF    L  +CCG+GG YNFNL + CG  G
Sbjct: 274 RLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL-SACCGVGGPYNFNLSRSCGSVG 333

Query: 302 VPVCPNPNEHISWDGVHLTQKAYKFMAHWLI 328
           V  C +P+++++WDG+H+T+ A+K MA  L+
Sbjct: 334 VEACSDPSKYVAWDGLHMTEAAHKSMADGLV 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3MKY21.6e-8244.38Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1[more]
Q94F402.6e-7743.71GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2... [more]
Q9LZB27.0e-7545.07GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2... [more]
Q8RXT99.2e-7542.90GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2... [more]
Q9SHP62.0e-7443.07GDSL esterase/lipase At1g28610 OS=Arabidopsis thaliana OX=3702 GN=At1g28610 PE=2... [more]
Match NameE-valueIdentityDescription
A0A1S4E6292.3e-18590.86acetylajmalan esterase-like OS=Cucumis melo OX=3656 GN=LOC103503259 PE=3 SV=1[more]
A0A5D3CNR68.6e-18590.56Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A5A7SSW21.9e-18490.56Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A5A7TWR02.5e-18490.27Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A5A7VJA53.6e-18389.97Acetylajmalan esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
XP_016903430.14.7e-18590.86PREDICTED: acetylajmalan esterase-like [Cucumis melo][more]
TYK13517.11.8e-18490.56acetylajmalan esterase-like [Cucumis melo var. makuwa][more]
XP_011659746.13.0e-18490.27acetylajmalan esterase-like [Cucumis sativus][more]
KAE8646130.13.0e-18490.27hypothetical protein Csa_016115 [Cucumis sativus][more]
KAA0032295.14.0e-18490.56acetylajmalan esterase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G28600.11.8e-7843.71GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03980.15.0e-7645.07SGNH hydrolase-type esterase superfamily protein [more]
AT1G28590.16.5e-7642.90GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G28610.21.5e-7543.07GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G31550.21.5e-7543.50GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 5..326
e-value: 7.2E-32
score: 111.6
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 1..330
e-value: 5.3E-66
score: 225.2
NoneNo IPR availablePANTHERPTHR22835:SF581ACETYLAJMALAN ESTERASE-LIKEcoord: 2..336
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 2..336
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 5..328
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 4..330
e-value: 2.72449E-109
score: 319.561

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G006010.1Lsi11G006010.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds